Ggra5145.t1 (mRNA) Gracilaria gracilis GNS1m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	Ggra5145.t1
Unique Name	Ggra5145.t1
Type	mRNA
Organism	Gracilaria gracilis GNS1m male (Gracilaria gracilis GNS1m male (Slender Wart Weed))
Sequence length	820

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
tig00000069_pilon	contig	tig00000069_pilon:592171..594630 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Gracilaria gracilis GNS1m male OGS1.0	2022-05-09

Properties


Property Name	Value
Seed ortholog	2850.Phatr48253
PFAMs	BACK,BTB,Kelch_1,Kelch_4,Kelch_6
Max annot lvl	2759\|Eukaryota
KEGG ko	ko:K10442,ko:K20285,ko:K21912
Evalue	1.91e-22
EggNOG OGs	KOG4441@1\|root,KOG4441@2759\|Eukaryota
Description	protein ubiquitination
COG category	KLT
BRITE	ko00000,ko04121,ko04131

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
Ggra5145	Ggra5145	Gracilaria gracilis GNS1m male	gene	tig00000069_pilon 592171..594630 -

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra5145.t1.stop1	Ggra5145.t1.stop1	Gracilaria gracilis GNS1m male	stop_codon	tig00000069_pilon 592171..592173 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra5145.t1.CDS1	Ggra5145.t1.CDS1	Gracilaria gracilis GNS1m male	CDS	tig00000069_pilon 592171..594630 -

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra5145.t1.exon1	Ggra5145.t1.exon1	Gracilaria gracilis GNS1m male	exon	tig00000069_pilon 592171..594630 -

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra5145.t1.start1	Ggra5145.t1.start1	Gracilaria gracilis GNS1m male	start_codon	tig00000069_pilon 594628..594630 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra5145.t1	Ggra5145.t1	Gracilaria gracilis GNS1m male	polypeptide	tig00000069_pilon 592171..594630 -

Sequences

The following sequences are available for this feature:

mRNA sequence
mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at tig00000069_pilon:592171..594630-
coding sequence from alignment at tig00000069_pilon:592171..594630-

mRNA sequence

>Ggra5145.t1 ID=Ggra5145.t1|Name=Ggra5145.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=820bp
MSCVKPFWLACLACLLLCVAAETPLRQLSFLPKGPGRPAFVAPQIQLPTL
PSSSTSDAPPAAPAPAPASAPAPAPAPALADAPPAQAEADTDAAQDAVDA
DVVDTDAIDTDAVNVDVDVAQVDSVESSETQQSPAAVPSASPSASPPPSV
SPVALAPTVSPAPSAAPVADAVLAPVVQPPPSVLTPIPTAAGNGQGYFPC
HPNKATKQTTVTCTHVQMPPAMCSRCALKPVTAQGMFKRCDDIFELETPP
CQQALDEYVALNGACDPVRTSHVAAWKAGDAAAATELDYFLYSVCELCCD
CVPMGTAVDEYASLKAAHTPQNPTLWAVDRGNCPAHAEYDLCNVLPNVQA
VIKPGEPVIAKPSACPPLSVWLNSDRSTNWQVNPDASIDASTRDFLNSAL
NALECDGESVWRTCYHMEDLQNHIDIPQDVASVPVQDDTAAAQLTTPAVV
TDPTAAADPVTPAAVPVSPATPATAAPAAPVPAAPVPAAPEPAAPEPAAP
EPAAPEPAAPEPAAAAPSAPTPVAPAPAAPAPAAPAPAAPAPAAPASPAT
ATDKDSATADAPASTAPITPIAATTTTDTASTSTTSAPDDTTTALPASDG
ETTDGASPMTVGGQENGIYGSPSPSPASDGGSCFPASATVELHSGLHIPM
HQLRIGDRVRVSADQFSEVYMFSHRDVKASDPSYVRIFTMEASVTMTAGH
YLPVVKNVTRTGNIFVQAESVEIGDRLQLASGQWSFVTDVLPTEAIGLFN
PHTLAGTICVDGVVASTFTRSVSADAAQALLSPIRALYWLGIAGERLAGN
WFVQGVDRSLTSFAQTIRL*

spliced messenger RNA

>Ggra5145.t1 ID=Ggra5145.t1|Name=Ggra5145.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=2460bp|location=Sequence derived from alignment at tig00000069_pilon:592171..594630- (Gracilaria gracilis GNS1m male)|Notes=Excludes all bases but those of type(s): exon.  
ATGAGCTGTGTCAAGCCCTTCTGGCTCGCTTGCCTTGCTTGCTTGCTGCT
TTGCGTCGCCGCCGAGACGCCGCTGCGACAGCTCTCTTTTTTGCCAAAGG
GACCGGGGCGTCCTGCCTTTGTTGCGCCTCAAATTCAGCTTCCCACTCTG
CCGTCATCGTCCACATCCGACGCGCCGCCTGCTGCACCAGCTCCCGCTCC
CGCTTCCGCTCCTGCGCCTGCGCCTGCGCCTGCTCTTGCTGACGCCCCGC
CGGCTCAAGCAGAGGCTGATACCGACGCTGCTCAAGATGCCGTCGATGCC
GATGTCGTCGATACCGATGCCATCGATACTGATGCCGTCAATGTCGATGT
CGATGTCGCCCAAGTCGACTCCGTTGAATCGTCCGAAACTCAGCAGTCAC
CAGCTGCTGTACCATCTGCCTCACCATCAGCGTCGCCACCGCCTTCCGTA
TCTCCAGTTGCATTAGCACCCACCGTATCGCCTGCACCTTCTGCTGCTCC
TGTTGCTGATGCTGTTCTCGCTCCTGTCGTACAGCCACCGCCGTCTGTGC
TTACCCCAATTCCCACCGCCGCTGGCAATGGCCAGGGTTACTTCCCGTGC
CATCCGAACAAGGCCACCAAGCAGACCACCGTCACCTGTACGCATGTTCA
AATGCCCCCAGCCATGTGTTCGCGATGCGCTTTGAAGCCGGTTACCGCTC
AAGGCATGTTCAAGCGTTGCGACGATATCTTTGAGCTTGAAACACCGCCT
TGTCAACAAGCGCTTGACGAATATGTCGCTCTTAATGGCGCTTGTGACCC
TGTTAGAACGAGCCATGTAGCCGCGTGGAAGGCCGGAGACGCCGCCGCTG
CCACAGAGCTTGATTACTTTTTGTACTCTGTTTGTGAGCTTTGCTGCGAT
TGTGTTCCGATGGGTACTGCTGTCGATGAGTATGCGTCGTTGAAGGCCGC
TCACACGCCGCAAAACCCAACGCTCTGGGCTGTTGATAGGGGAAATTGTC
CGGCTCATGCCGAGTATGACCTTTGCAACGTGCTCCCCAATGTGCAAGCT
GTTATCAAGCCTGGCGAGCCTGTAATCGCAAAACCGTCAGCTTGTCCGCC
TCTGAGCGTTTGGCTCAATTCGGACCGCTCTACAAACTGGCAAGTCAATC
CAGATGCCTCAATCGACGCTTCCACCAGGGATTTCTTGAACAGCGCTCTC
AATGCATTGGAATGCGATGGCGAGAGCGTTTGGCGCACTTGCTATCATAT
GGAGGATTTGCAAAACCACATTGACATTCCCCAAGATGTTGCTTCTGTTC
CTGTGCAGGATGATACTGCCGCCGCACAGCTTACTACACCTGCTGTCGTT
ACCGACCCTACCGCCGCTGCAGATCCTGTCACCCCTGCAGCGGTTCCTGT
TAGCCCTGCAACCCCTGCCACTGCTGCGCCTGCCGCTCCCGTGCCTGCCG
CTCCCGTGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCCGCTCCC
GAGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCAGCTGCTGCGCC
GTCGGCTCCTACGCCTGTGGCTCCTGCGCCTGCAGCTCCTGCTCCCGCAG
CTCCCGCTCCCGCAGCTCCCGCTCCTGCGGCGCCTGCGTCACCTGCGACA
GCTACTGACAAAGACTCCGCTACAGCTGATGCCCCCGCTTCTACAGCACC
TATCACTCCCATTGCTGCTACCACGACTACTGACACTGCAAGTACATCAA
CTACTTCTGCACCAGATGATACCACTACTGCTCTTCCGGCATCTGACGGC
GAGACCACTGATGGGGCGTCGCCGATGACTGTTGGTGGCCAAGAGAATGG
AATATATGGTTCTCCGTCTCCTTCTCCTGCCAGCGATGGAGGCTCCTGTT
TCCCTGCCAGCGCTACAGTTGAGCTCCACTCTGGATTACACATTCCGATG
CACCAACTCAGAATTGGTGATCGTGTTCGCGTGTCTGCTGATCAGTTTTC
AGAGGTTTACATGTTTTCACACCGCGATGTCAAAGCTTCAGATCCGTCAT
ACGTACGCATCTTCACGATGGAGGCTTCTGTAACAATGACAGCAGGACAC
TACCTGCCAGTCGTCAAGAACGTCACAAGAACAGGCAACATATTTGTACA
GGCAGAGTCCGTTGAGATTGGAGATCGTTTGCAGCTTGCTTCTGGGCAAT
GGTCGTTTGTAACTGATGTTCTGCCGACTGAAGCAATTGGTTTGTTCAAC
CCCCATACTTTGGCGGGTACTATCTGTGTGGATGGTGTGGTGGCATCGAC
TTTCACTCGTTCCGTGTCTGCAGATGCTGCGCAGGCCTTGTTATCGCCGA
TTAGAGCCTTGTATTGGTTGGGAATTGCTGGTGAGAGACTCGCAGGGAAC
TGGTTTGTGCAAGGTGTTGATCGAAGCTTGACATCCTTTGCTCAAACAAT
TCGACTGTGA

protein sequence of Ggra5145.t1

>Ggra5145.t1 ID=Ggra5145.t1|Name=Ggra5145.t1|organism=Gracilaria gracilis GNS1m male|type=polypeptide|length=820bp
MSCVKPFWLACLACLLLCVAAETPLRQLSFLPKGPGRPAFVAPQIQLPTL
PSSSTSDAPPAAPAPAPASAPAPAPAPALADAPPAQAEADTDAAQDAVDA
DVVDTDAIDTDAVNVDVDVAQVDSVESSETQQSPAAVPSASPSASPPPSV
SPVALAPTVSPAPSAAPVADAVLAPVVQPPPSVLTPIPTAAGNGQGYFPC
HPNKATKQTTVTCTHVQMPPAMCSRCALKPVTAQGMFKRCDDIFELETPP
CQQALDEYVALNGACDPVRTSHVAAWKAGDAAAATELDYFLYSVCELCCD
CVPMGTAVDEYASLKAAHTPQNPTLWAVDRGNCPAHAEYDLCNVLPNVQA
VIKPGEPVIAKPSACPPLSVWLNSDRSTNWQVNPDASIDASTRDFLNSAL
NALECDGESVWRTCYHMEDLQNHIDIPQDVASVPVQDDTAAAQLTTPAVV
TDPTAAADPVTPAAVPVSPATPATAAPAAPVPAAPVPAAPEPAAPEPAAP
EPAAPEPAAPEPAAAAPSAPTPVAPAPAAPAPAAPAPAAPAPAAPASPAT
ATDKDSATADAPASTAPITPIAATTTTDTASTSTTSAPDDTTTALPASDG
ETTDGASPMTVGGQENGIYGSPSPSPASDGGSCFPASATVELHSGLHIPM
HQLRIGDRVRVSADQFSEVYMFSHRDVKASDPSYVRIFTMEASVTMTAGH
YLPVVKNVTRTGNIFVQAESVEIGDRLQLASGQWSFVTDVLPTEAIGLFN
PHTLAGTICVDGVVASTFTRSVSADAAQALLSPIRALYWLGIAGERLAGN
WFVQGVDRSLTSFAQTIRL*

mRNA from alignment at tig00000069_pilon:592171..594630-

Legend: polypeptideCDSexonstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

>Ggra5145.t1 ID=Ggra5145.t1|Name=Ggra5145.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=2460bp|location=Sequence derived from alignment at tig00000069_pilon:592171..594630- (Gracilaria gracilis GNS1m male)
ATGAGCTGTGTCAAGCCCTTCTGGCTCGCTTGCCTTGCTTGCTTGCTGCT
TTGCGTCGCCGCCGAGACGCCGCTGCGACAGCTCTCTTTTTTGCCAAAGG
GACCGGGGCGTCCTGCCTTTGTTGCGCCTCAAATTCAGCTTCCCACTCTG
CCGTCATCGTCCACATCCGACGCGCCGCCTGCTGCACCAGCTCCCGCTCC
CGCTTCCGCTCCTGCGCCTGCGCCTGCGCCTGCTCTTGCTGACGCCCCGC
CGGCTCAAGCAGAGGCTGATACCGACGCTGCTCAAGATGCCGTCGATGCC
GATGTCGTCGATACCGATGCCATCGATACTGATGCCGTCAATGTCGATGT
CGATGTCGCCCAAGTCGACTCCGTTGAATCGTCCGAAACTCAGCAGTCAC
CAGCTGCTGTACCATCTGCCTCACCATCAGCGTCGCCACCGCCTTCCGTA
TCTCCAGTTGCATTAGCACCCACCGTATCGCCTGCACCTTCTGCTGCTCC
TGTTGCTGATGCTGTTCTCGCTCCTGTCGTACAGCCACCGCCGTCTGTGC
TTACCCCAATTCCCACCGCCGCTGGCAATGGCCAGGGTTACTTCCCGTGC
CATCCGAACAAGGCCACCAAGCAGACCACCGTCACCTGTACGCATGTTCA
AATGCCCCCAGCCATGTGTTCGCGATGCGCTTTGAAGCCGGTTACCGCTC
AAGGCATGTTCAAGCGTTGCGACGATATCTTTGAGCTTGAAACACCGCCT
TGTCAACAAGCGCTTGACGAATATGTCGCTCTTAATGGCGCTTGTGACCC
TGTTAGAACGAGCCATGTAGCCGCGTGGAAGGCCGGAGACGCCGCCGCTG
CCACAGAGCTTGATTACTTTTTGTACTCTGTTTGTGAGCTTTGCTGCGAT
TGTGTTCCGATGGGTACTGCTGTCGATGAGTATGCGTCGTTGAAGGCCGC
TCACACGCCGCAAAACCCAACGCTCTGGGCTGTTGATAGGGGAAATTGTC
CGGCTCATGCCGAGTATGACCTTTGCAACGTGCTCCCCAATGTGCAAGCT
GTTATCAAGCCTGGCGAGCCTGTAATCGCAAAACCGTCAGCTTGTCCGCC
TCTGAGCGTTTGGCTCAATTCGGACCGCTCTACAAACTGGCAAGTCAATC
CAGATGCCTCAATCGACGCTTCCACCAGGGATTTCTTGAACAGCGCTCTC
AATGCATTGGAATGCGATGGCGAGAGCGTTTGGCGCACTTGCTATCATAT
GGAGGATTTGCAAAACCACATTGACATTCCCCAAGATGTTGCTTCTGTTC
CTGTGCAGGATGATACTGCCGCCGCACAGCTTACTACACCTGCTGTCGTT
ACCGACCCTACCGCCGCTGCAGATCCTGTCACCCCTGCAGCGGTTCCTGT
TAGCCCTGCAACCCCTGCCACTGCTGCGCCTGCCGCTCCCGTGCCTGCCG
CTCCCGTGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCCGCTCCC
GAGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCAGCTGCTGCGCC
GTCGGCTCCTACGCCTGTGGCTCCTGCGCCTGCAGCTCCTGCTCCCGCAG
CTCCCGCTCCCGCAGCTCCCGCTCCTGCGGCGCCTGCGTCACCTGCGACA
GCTACTGACAAAGACTCCGCTACAGCTGATGCCCCCGCTTCTACAGCACC
TATCACTCCCATTGCTGCTACCACGACTACTGACACTGCAAGTACATCAA
CTACTTCTGCACCAGATGATACCACTACTGCTCTTCCGGCATCTGACGGC
GAGACCACTGATGGGGCGTCGCCGATGACTGTTGGTGGCCAAGAGAATGG
AATATATGGTTCTCCGTCTCCTTCTCCTGCCAGCGATGGAGGCTCCTGTT
TCCCTGCCAGCGCTACAGTTGAGCTCCACTCTGGATTACACATTCCGATG
CACCAACTCAGAATTGGTGATCGTGTTCGCGTGTCTGCTGATCAGTTTTC
AGAGGTTTACATGTTTTCACACCGCGATGTCAAAGCTTCAGATCCGTCAT
ACGTACGCATCTTCACGATGGAGGCTTCTGTAACAATGACAGCAGGACAC
TACCTGCCAGTCGTCAAGAACGTCACAAGAACAGGCAACATATTTGTACA
GGCAGAGTCCGTTGAGATTGGAGATCGTTTGCAGCTTGCTTCTGGGCAAT
GGTCGTTTGTAACTGATGTTCTGCCGACTGAAGCAATTGGTTTGTTCAAC
CCCCATACTTTGGCGGGTACTATCTGTGTGGATGGTGTGGTGGCATCGAC
TTTCACTCGTTCCGTGTCTGCAGATGCTGCGCAGGCCTTGTTATCGCCGA
TTAGAGCCTTGTATTGGTTGGGAATTGCTGGTGAGAGACTCGCAGGGAAC
TGGTTTGTGCAAGGTGTTGATCGAAGCTTGACATCCTTTGCTCAAACAAT
TCGACTGTGA

Coding sequence (CDS) from alignment at tig00000069_pilon:592171..594630-

>Ggra5145.t1 ID=Ggra5145.t1|Name=Ggra5145.t1|organism=Gracilaria gracilis GNS1m male|type=CDS|length=2460bp|location=Sequence derived from alignment at tig00000069_pilon:592171..594630- (Gracilaria gracilis GNS1m male)
ATGAGCTGTGTCAAGCCCTTCTGGCTCGCTTGCCTTGCTTGCTTGCTGCT
TTGCGTCGCCGCCGAGACGCCGCTGCGACAGCTCTCTTTTTTGCCAAAGG
GACCGGGGCGTCCTGCCTTTGTTGCGCCTCAAATTCAGCTTCCCACTCTG
CCGTCATCGTCCACATCCGACGCGCCGCCTGCTGCACCAGCTCCCGCTCC
CGCTTCCGCTCCTGCGCCTGCGCCTGCGCCTGCTCTTGCTGACGCCCCGC
CGGCTCAAGCAGAGGCTGATACCGACGCTGCTCAAGATGCCGTCGATGCC
GATGTCGTCGATACCGATGCCATCGATACTGATGCCGTCAATGTCGATGT
CGATGTCGCCCAAGTCGACTCCGTTGAATCGTCCGAAACTCAGCAGTCAC
CAGCTGCTGTACCATCTGCCTCACCATCAGCGTCGCCACCGCCTTCCGTA
TCTCCAGTTGCATTAGCACCCACCGTATCGCCTGCACCTTCTGCTGCTCC
TGTTGCTGATGCTGTTCTCGCTCCTGTCGTACAGCCACCGCCGTCTGTGC
TTACCCCAATTCCCACCGCCGCTGGCAATGGCCAGGGTTACTTCCCGTGC
CATCCGAACAAGGCCACCAAGCAGACCACCGTCACCTGTACGCATGTTCA
AATGCCCCCAGCCATGTGTTCGCGATGCGCTTTGAAGCCGGTTACCGCTC
AAGGCATGTTCAAGCGTTGCGACGATATCTTTGAGCTTGAAACACCGCCT
TGTCAACAAGCGCTTGACGAATATGTCGCTCTTAATGGCGCTTGTGACCC
TGTTAGAACGAGCCATGTAGCCGCGTGGAAGGCCGGAGACGCCGCCGCTG
CCACAGAGCTTGATTACTTTTTGTACTCTGTTTGTGAGCTTTGCTGCGAT
TGTGTTCCGATGGGTACTGCTGTCGATGAGTATGCGTCGTTGAAGGCCGC
TCACACGCCGCAAAACCCAACGCTCTGGGCTGTTGATAGGGGAAATTGTC
CGGCTCATGCCGAGTATGACCTTTGCAACGTGCTCCCCAATGTGCAAGCT
GTTATCAAGCCTGGCGAGCCTGTAATCGCAAAACCGTCAGCTTGTCCGCC
TCTGAGCGTTTGGCTCAATTCGGACCGCTCTACAAACTGGCAAGTCAATC
CAGATGCCTCAATCGACGCTTCCACCAGGGATTTCTTGAACAGCGCTCTC
AATGCATTGGAATGCGATGGCGAGAGCGTTTGGCGCACTTGCTATCATAT
GGAGGATTTGCAAAACCACATTGACATTCCCCAAGATGTTGCTTCTGTTC
CTGTGCAGGATGATACTGCCGCCGCACAGCTTACTACACCTGCTGTCGTT
ACCGACCCTACCGCCGCTGCAGATCCTGTCACCCCTGCAGCGGTTCCTGT
TAGCCCTGCAACCCCTGCCACTGCTGCGCCTGCCGCTCCCGTGCCTGCCG
CTCCCGTGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCCGCTCCC
GAGCCTGCCGCTCCCGAGCCTGCCGCTCCCGAGCCTGCAGCTGCTGCGCC
GTCGGCTCCTACGCCTGTGGCTCCTGCGCCTGCAGCTCCTGCTCCCGCAG
CTCCCGCTCCCGCAGCTCCCGCTCCTGCGGCGCCTGCGTCACCTGCGACA
GCTACTGACAAAGACTCCGCTACAGCTGATGCCCCCGCTTCTACAGCACC
TATCACTCCCATTGCTGCTACCACGACTACTGACACTGCAAGTACATCAA
CTACTTCTGCACCAGATGATACCACTACTGCTCTTCCGGCATCTGACGGC
GAGACCACTGATGGGGCGTCGCCGATGACTGTTGGTGGCCAAGAGAATGG
AATATATGGTTCTCCGTCTCCTTCTCCTGCCAGCGATGGAGGCTCCTGTT
TCCCTGCCAGCGCTACAGTTGAGCTCCACTCTGGATTACACATTCCGATG
CACCAACTCAGAATTGGTGATCGTGTTCGCGTGTCTGCTGATCAGTTTTC
AGAGGTTTACATGTTTTCACACCGCGATGTCAAAGCTTCAGATCCGTCAT
ACGTACGCATCTTCACGATGGAGGCTTCTGTAACAATGACAGCAGGACAC
TACCTGCCAGTCGTCAAGAACGTCACAAGAACAGGCAACATATTTGTACA
GGCAGAGTCCGTTGAGATTGGAGATCGTTTGCAGCTTGCTTCTGGGCAAT
GGTCGTTTGTAACTGATGTTCTGCCGACTGAAGCAATTGGTTTGTTCAAC
CCCCATACTTTGGCGGGTACTATCTGTGTGGATGGTGTGGTGGCATCGAC
TTTCACTCGTTCCGTGTCTGCAGATGCTGCGCAGGCCTTGTTATCGCCGA
TTAGAGCCTTGTATTGGTTGGGAATTGCTGGTGAGAGACTCGCAGGGAAC
TGGTTTGTGCAAGGTGTTGATCGAAGCTTGACATCCTTTGCTCAAACAAT
TCGACTGTGA