Ggra2418.t1 (mRNA) Gracilaria gracilis GNS1m male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	Ggra2418.t1
Unique Name	Ggra2418.t1
Type	mRNA
Organism	Gracilaria gracilis GNS1m male (Gracilaria gracilis GNS1m male (Slender Wart Weed))
Sequence length	744

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
tig00000005_pilon	contig	tig00000005_pilon:223546..225777 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Gracilaria gracilis GNS1m male OGS1.0	2022-05-09

Properties


Property Name	Value
Seed ortholog	130081.XP_005702872.1
Preferred name	LSS
PFAMs	SQHop_cyclase_C,SQHop_cyclase_N
Max annot lvl	2759\|Eukaryota
KEGG rclass	RC00874,RC01582,RC02604
KEGG ko	ko:K01852,ko:K01853,ko:K15811
KEGG Reaction	R03199,R03200,R09689
KEGG Pathway	ko00100,ko00909,ko01100,ko01110,ko01130,map00100,map00909,map01100,map01110,map01130
KEGG Module	M00101
GOs	GO:0000250,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0005811,GO:0006066,GO:0006629,GO:0006694,GO:0006695,GO:0006696,GO:0006720,GO:0006721,GO:0006722,GO:0008150,GO:0008152,GO:0008202,GO:0008203,GO:0008204,GO:0008299,GO:0008610,GO:0009058,GO:0009889,GO:0009987,GO:0010685,GO:0010686,GO:0012505,GO:0016020,GO:0016104,GO:0016114,GO:0016125,GO:0016126,GO:0016128,GO:0016129,GO:0016853,GO:0016866,GO:0019216,GO:0019218,GO:0019222,GO:0019748,GO:0031559,GO:0031647,GO:0031984,GO:0042175,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0044107,GO:0044108,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044281,GO:0044283,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0044550,GO:0045540,GO:0046165,GO:0046890,GO:0050789,GO:0050810,GO:0062012,GO:0065007,GO:0065008,GO:0071704,GO:0080090,GO:0090181,GO:0097384,GO:0098827,GO:0106118,GO:1900579,GO:1900581,GO:1901360,GO:1901362,GO:1901576,GO:1901615,GO:1901617,GO:1902652,GO:1902653,GO:1902930
Evalue	1.19e-282
EggNOG OGs	COG1657@1\|root,KOG0497@2759\|Eukaryota
EC	5.4.99.32,5.4.99.7,5.4.99.8
Description	oxidosqualene cyclase activity
COG category	I
BiGG Reaction	iMM904.YHR072W,iND750.YHR072W
BRITE	ko00000,ko00001,ko00002,ko01000

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
Ggra2418	Ggra2418	Gracilaria gracilis GNS1m male	gene	tig00000005_pilon 223546..225777 -

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra2418.t1.stop1	Ggra2418.t1.stop1	Gracilaria gracilis GNS1m male	stop_codon	tig00000005_pilon 223546..223548 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra2418.t1.CDS1	Ggra2418.t1.CDS1	Gracilaria gracilis GNS1m male	CDS	tig00000005_pilon 223546..225777 -

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra2418.t1.exon1	Ggra2418.t1.exon1	Gracilaria gracilis GNS1m male	exon	tig00000005_pilon 223546..225777 -

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra2418.t1.start1	Ggra2418.t1.start1	Gracilaria gracilis GNS1m male	start_codon	tig00000005_pilon 225775..225777 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
Ggra2418.t1	Ggra2418.t1	Gracilaria gracilis GNS1m male	polypeptide	tig00000005_pilon 223546..225777 -

Sequences

The following sequences are available for this feature:

mRNA sequence
mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at tig00000005_pilon:223546..225777-
coding sequence from alignment at tig00000005_pilon:223546..225777-

mRNA sequence

>Ggra2418.t1 ID=Ggra2418.t1|Name=Ggra2418.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=744bp
MGAVEGESDMWMLRFERGRQWWEPNGPQDESHLKDLKEAQTNFASNRQKT
SHSSDRVLRLLGAHGLIEGNPTLGRIEPVPGHIAGDPVSVAIHKGIAYFA
TLQCGDGHWAGDYAGPMFLMPGMIIACYVTKTPLHPSQKREMIRYLRNHQ
NKDGGFGLHIEHKSIMFGTGLNYVALRLLGVGPDDPDAVRARQWILDHGG
AGGIPGWGKFWLSVLGVYDWEGVDPLTPEFWLMPYAIPMHPARFWCHCRV
VYLPMSYLYGRKAVGETTNLIKDIREELYGEPYSEINWASFRGKCCEEDV
YVKRPYLQRLLWGALVIFEKTWFPFKKVLRQKALQETLELVKGEDRFSKH
IDIGPVNKVINFLCVWFDNPNSEEVSKHRDRLKDYLWLAEDGMKMQGYNG
SQLWDTAFTSQAFMTAGEEILRPFVRTLSLAHDYIRITQVREDVPNRERY
YRHPSKGAWPFSTRDHGWPIADCTGEGLAASLLLQGEEGSWIPKTQCISL
ERLADAAEMILSYQNEDGGWATYELTRGPRWLEYLNPSEVFGDIMIDYSY
VECSASAMKGLGEFRKAYPNHPLRPRIDRSVERGIKFIESIQREDGSWYG
SWGVCFLYAFWFAMDAYSSVGLTPDSSSSMRRACEFILSKQKEDGSWGES
YLSSEQLVYVQSKHGLVVSTGWALVALSMARWFNKEPLEKAAQFLIRSQE
ENGDWPQQNICGVFNKNCMISYSQYRNIFPIWALSEYRRYCTS*

spliced messenger RNA

>Ggra2418.t1 ID=Ggra2418.t1|Name=Ggra2418.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=2232bp|location=Sequence derived from alignment at tig00000005_pilon:223546..225777- (Gracilaria gracilis GNS1m male)|Notes=Excludes all bases but those of type(s): exon.  
ATGGGGGCTGTCGAAGGCGAATCGGATATGTGGATGCTCCGCTTCGAAAG
AGGGAGGCAGTGGTGGGAACCTAATGGACCCCAAGACGAGAGCCACCTCA
AGGACTTGAAAGAAGCGCAAACGAACTTTGCTAGCAATCGTCAGAAAACG
AGTCATTCCTCAGATCGCGTTCTTCGTTTGCTCGGTGCTCATGGTTTGAT
CGAAGGTAATCCTACCCTTGGAAGAATCGAACCAGTCCCTGGTCATATCG
CAGGCGATCCTGTGTCTGTAGCAATTCACAAAGGTATCGCATATTTTGCA
ACGCTTCAGTGTGGAGATGGACATTGGGCAGGTGACTATGCTGGACCGAT
GTTCCTCATGCCTGGTATGATTATAGCATGTTATGTGACCAAGACACCTT
TGCATCCCTCACAAAAGCGTGAAATGATCCGTTATTTACGAAACCATCAG
AACAAAGATGGTGGTTTCGGTTTGCATATCGAACATAAGTCAATAATGTT
TGGGACGGGACTCAATTATGTTGCCCTGAGGCTGCTAGGAGTAGGCCCTG
ATGACCCTGATGCGGTGCGTGCGAGACAATGGATCCTAGACCATGGTGGA
GCAGGGGGAATCCCCGGTTGGGGAAAGTTCTGGCTGTCGGTCTTGGGCGT
CTACGATTGGGAGGGCGTGGATCCGTTAACACCAGAGTTCTGGCTGATGC
CATATGCAATTCCAATGCACCCGGCTCGGTTTTGGTGTCATTGTAGAGTG
GTTTACCTTCCTATGAGCTATTTGTATGGTAGGAAAGCTGTCGGTGAGAC
GACAAACCTCATCAAAGATATTCGAGAAGAATTGTACGGAGAGCCGTATA
GCGAGATAAACTGGGCAAGTTTCAGGGGAAAGTGTTGCGAAGAAGATGTC
TACGTGAAGAGGCCGTATCTCCAAAGACTGTTGTGGGGTGCTCTCGTTAT
CTTTGAGAAAACATGGTTTCCGTTCAAGAAAGTATTGAGGCAAAAAGCGC
TGCAGGAAACACTTGAGCTTGTCAAAGGAGAAGATCGATTTTCTAAGCAC
ATCGATATCGGTCCGGTCAATAAGGTTATTAACTTCCTTTGCGTCTGGTT
TGATAACCCCAATAGTGAAGAGGTGTCAAAACACCGTGACAGACTGAAGG
ATTATCTGTGGCTTGCTGAAGATGGGATGAAAATGCAGGGATATAATGGT
TCTCAACTTTGGGACACCGCGTTCACCAGTCAAGCTTTCATGACGGCCGG
GGAAGAAATCCTACGTCCTTTTGTCAGAACATTGTCGCTAGCTCATGATT
ATATCAGAATCACGCAAGTCAGAGAGGATGTTCCCAATCGCGAAAGATAT
TACCGACATCCAAGCAAAGGGGCATGGCCTTTCAGCACAAGAGATCATGG
TTGGCCAATTGCAGATTGCACTGGAGAAGGGCTAGCAGCGTCGTTACTCT
TGCAAGGTGAGGAAGGGTCGTGGATTCCAAAAACTCAGTGCATATCGTTA
GAACGACTTGCAGACGCTGCCGAAATGATTCTCAGTTACCAAAATGAAGA
TGGTGGTTGGGCTACCTATGAGCTTACTCGTGGTCCCAGATGGCTCGAGT
ATCTAAATCCGTCTGAAGTGTTCGGAGATATCATGATTGATTACTCCTAT
GTTGAATGCTCTGCTTCAGCCATGAAAGGTTTGGGGGAGTTTCGGAAAGC
TTACCCTAACCATCCGTTGAGGCCCAGAATCGACAGATCAGTCGAAAGAG
GAATCAAGTTTATTGAGTCCATTCAAAGGGAAGATGGCTCATGGTATGGA
AGCTGGGGGGTTTGCTTTCTGTATGCGTTCTGGTTTGCAATGGACGCGTA
CTCTTCTGTTGGGCTCACACCTGACTCGAGTAGTTCTATGCGACGGGCAT
GTGAATTTATTTTGAGCAAACAGAAGGAAGACGGTAGCTGGGGAGAGAGC
TATCTCAGTTCTGAACAATTGGTCTATGTGCAGTCAAAACATGGGCTTGT
TGTTAGTACGGGTTGGGCGCTAGTGGCATTAAGCATGGCGAGATGGTTTA
ACAAGGAGCCACTGGAGAAGGCTGCGCAGTTTCTCATTCGCTCGCAAGAA
GAAAATGGAGACTGGCCACAGCAAAACATCTGTGGTGTCTTTAACAAGAA
CTGTATGATATCATATTCCCAATACCGTAACATCTTTCCAATTTGGGCTC
TTTCTGAATATCGAAGGTATTGTACATCATGA

protein sequence of Ggra2418.t1

>Ggra2418.t1 ID=Ggra2418.t1|Name=Ggra2418.t1|organism=Gracilaria gracilis GNS1m male|type=polypeptide|length=744bp
MGAVEGESDMWMLRFERGRQWWEPNGPQDESHLKDLKEAQTNFASNRQKT
SHSSDRVLRLLGAHGLIEGNPTLGRIEPVPGHIAGDPVSVAIHKGIAYFA
TLQCGDGHWAGDYAGPMFLMPGMIIACYVTKTPLHPSQKREMIRYLRNHQ
NKDGGFGLHIEHKSIMFGTGLNYVALRLLGVGPDDPDAVRARQWILDHGG
AGGIPGWGKFWLSVLGVYDWEGVDPLTPEFWLMPYAIPMHPARFWCHCRV
VYLPMSYLYGRKAVGETTNLIKDIREELYGEPYSEINWASFRGKCCEEDV
YVKRPYLQRLLWGALVIFEKTWFPFKKVLRQKALQETLELVKGEDRFSKH
IDIGPVNKVINFLCVWFDNPNSEEVSKHRDRLKDYLWLAEDGMKMQGYNG
SQLWDTAFTSQAFMTAGEEILRPFVRTLSLAHDYIRITQVREDVPNRERY
YRHPSKGAWPFSTRDHGWPIADCTGEGLAASLLLQGEEGSWIPKTQCISL
ERLADAAEMILSYQNEDGGWATYELTRGPRWLEYLNPSEVFGDIMIDYSY
VECSASAMKGLGEFRKAYPNHPLRPRIDRSVERGIKFIESIQREDGSWYG
SWGVCFLYAFWFAMDAYSSVGLTPDSSSSMRRACEFILSKQKEDGSWGES
YLSSEQLVYVQSKHGLVVSTGWALVALSMARWFNKEPLEKAAQFLIRSQE
ENGDWPQQNICGVFNKNCMISYSQYRNIFPIWALSEYRRYCTS*

mRNA from alignment at tig00000005_pilon:223546..225777-

Legend: polypeptideCDSexonstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

>Ggra2418.t1 ID=Ggra2418.t1|Name=Ggra2418.t1|organism=Gracilaria gracilis GNS1m male|type=mRNA|length=2232bp|location=Sequence derived from alignment at tig00000005_pilon:223546..225777- (Gracilaria gracilis GNS1m male)
ATGGGGGCTGTCGAAGGCGAATCGGATATGTGGATGCTCCGCTTCGAAAG
AGGGAGGCAGTGGTGGGAACCTAATGGACCCCAAGACGAGAGCCACCTCA
AGGACTTGAAAGAAGCGCAAACGAACTTTGCTAGCAATCGTCAGAAAACG
AGTCATTCCTCAGATCGCGTTCTTCGTTTGCTCGGTGCTCATGGTTTGAT
CGAAGGTAATCCTACCCTTGGAAGAATCGAACCAGTCCCTGGTCATATCG
CAGGCGATCCTGTGTCTGTAGCAATTCACAAAGGTATCGCATATTTTGCA
ACGCTTCAGTGTGGAGATGGACATTGGGCAGGTGACTATGCTGGACCGAT
GTTCCTCATGCCTGGTATGATTATAGCATGTTATGTGACCAAGACACCTT
TGCATCCCTCACAAAAGCGTGAAATGATCCGTTATTTACGAAACCATCAG
AACAAAGATGGTGGTTTCGGTTTGCATATCGAACATAAGTCAATAATGTT
TGGGACGGGACTCAATTATGTTGCCCTGAGGCTGCTAGGAGTAGGCCCTG
ATGACCCTGATGCGGTGCGTGCGAGACAATGGATCCTAGACCATGGTGGA
GCAGGGGGAATCCCCGGTTGGGGAAAGTTCTGGCTGTCGGTCTTGGGCGT
CTACGATTGGGAGGGCGTGGATCCGTTAACACCAGAGTTCTGGCTGATGC
CATATGCAATTCCAATGCACCCGGCTCGGTTTTGGTGTCATTGTAGAGTG
GTTTACCTTCCTATGAGCTATTTGTATGGTAGGAAAGCTGTCGGTGAGAC
GACAAACCTCATCAAAGATATTCGAGAAGAATTGTACGGAGAGCCGTATA
GCGAGATAAACTGGGCAAGTTTCAGGGGAAAGTGTTGCGAAGAAGATGTC
TACGTGAAGAGGCCGTATCTCCAAAGACTGTTGTGGGGTGCTCTCGTTAT
CTTTGAGAAAACATGGTTTCCGTTCAAGAAAGTATTGAGGCAAAAAGCGC
TGCAGGAAACACTTGAGCTTGTCAAAGGAGAAGATCGATTTTCTAAGCAC
ATCGATATCGGTCCGGTCAATAAGGTTATTAACTTCCTTTGCGTCTGGTT
TGATAACCCCAATAGTGAAGAGGTGTCAAAACACCGTGACAGACTGAAGG
ATTATCTGTGGCTTGCTGAAGATGGGATGAAAATGCAGGGATATAATGGT
TCTCAACTTTGGGACACCGCGTTCACCAGTCAAGCTTTCATGACGGCCGG
GGAAGAAATCCTACGTCCTTTTGTCAGAACATTGTCGCTAGCTCATGATT
ATATCAGAATCACGCAAGTCAGAGAGGATGTTCCCAATCGCGAAAGATAT
TACCGACATCCAAGCAAAGGGGCATGGCCTTTCAGCACAAGAGATCATGG
TTGGCCAATTGCAGATTGCACTGGAGAAGGGCTAGCAGCGTCGTTACTCT
TGCAAGGTGAGGAAGGGTCGTGGATTCCAAAAACTCAGTGCATATCGTTA
GAACGACTTGCAGACGCTGCCGAAATGATTCTCAGTTACCAAAATGAAGA
TGGTGGTTGGGCTACCTATGAGCTTACTCGTGGTCCCAGATGGCTCGAGT
ATCTAAATCCGTCTGAAGTGTTCGGAGATATCATGATTGATTACTCCTAT
GTTGAATGCTCTGCTTCAGCCATGAAAGGTTTGGGGGAGTTTCGGAAAGC
TTACCCTAACCATCCGTTGAGGCCCAGAATCGACAGATCAGTCGAAAGAG
GAATCAAGTTTATTGAGTCCATTCAAAGGGAAGATGGCTCATGGTATGGA
AGCTGGGGGGTTTGCTTTCTGTATGCGTTCTGGTTTGCAATGGACGCGTA
CTCTTCTGTTGGGCTCACACCTGACTCGAGTAGTTCTATGCGACGGGCAT
GTGAATTTATTTTGAGCAAACAGAAGGAAGACGGTAGCTGGGGAGAGAGC
TATCTCAGTTCTGAACAATTGGTCTATGTGCAGTCAAAACATGGGCTTGT
TGTTAGTACGGGTTGGGCGCTAGTGGCATTAAGCATGGCGAGATGGTTTA
ACAAGGAGCCACTGGAGAAGGCTGCGCAGTTTCTCATTCGCTCGCAAGAA
GAAAATGGAGACTGGCCACAGCAAAACATCTGTGGTGTCTTTAACAAGAA
CTGTATGATATCATATTCCCAATACCGTAACATCTTTCCAATTTGGGCTC
TTTCTGAATATCGAAGGTATTGTACATCATGA

Coding sequence (CDS) from alignment at tig00000005_pilon:223546..225777-

>Ggra2418.t1 ID=Ggra2418.t1|Name=Ggra2418.t1|organism=Gracilaria gracilis GNS1m male|type=CDS|length=2232bp|location=Sequence derived from alignment at tig00000005_pilon:223546..225777- (Gracilaria gracilis GNS1m male)
ATGGGGGCTGTCGAAGGCGAATCGGATATGTGGATGCTCCGCTTCGAAAG
AGGGAGGCAGTGGTGGGAACCTAATGGACCCCAAGACGAGAGCCACCTCA
AGGACTTGAAAGAAGCGCAAACGAACTTTGCTAGCAATCGTCAGAAAACG
AGTCATTCCTCAGATCGCGTTCTTCGTTTGCTCGGTGCTCATGGTTTGAT
CGAAGGTAATCCTACCCTTGGAAGAATCGAACCAGTCCCTGGTCATATCG
CAGGCGATCCTGTGTCTGTAGCAATTCACAAAGGTATCGCATATTTTGCA
ACGCTTCAGTGTGGAGATGGACATTGGGCAGGTGACTATGCTGGACCGAT
GTTCCTCATGCCTGGTATGATTATAGCATGTTATGTGACCAAGACACCTT
TGCATCCCTCACAAAAGCGTGAAATGATCCGTTATTTACGAAACCATCAG
AACAAAGATGGTGGTTTCGGTTTGCATATCGAACATAAGTCAATAATGTT
TGGGACGGGACTCAATTATGTTGCCCTGAGGCTGCTAGGAGTAGGCCCTG
ATGACCCTGATGCGGTGCGTGCGAGACAATGGATCCTAGACCATGGTGGA
GCAGGGGGAATCCCCGGTTGGGGAAAGTTCTGGCTGTCGGTCTTGGGCGT
CTACGATTGGGAGGGCGTGGATCCGTTAACACCAGAGTTCTGGCTGATGC
CATATGCAATTCCAATGCACCCGGCTCGGTTTTGGTGTCATTGTAGAGTG
GTTTACCTTCCTATGAGCTATTTGTATGGTAGGAAAGCTGTCGGTGAGAC
GACAAACCTCATCAAAGATATTCGAGAAGAATTGTACGGAGAGCCGTATA
GCGAGATAAACTGGGCAAGTTTCAGGGGAAAGTGTTGCGAAGAAGATGTC
TACGTGAAGAGGCCGTATCTCCAAAGACTGTTGTGGGGTGCTCTCGTTAT
CTTTGAGAAAACATGGTTTCCGTTCAAGAAAGTATTGAGGCAAAAAGCGC
TGCAGGAAACACTTGAGCTTGTCAAAGGAGAAGATCGATTTTCTAAGCAC
ATCGATATCGGTCCGGTCAATAAGGTTATTAACTTCCTTTGCGTCTGGTT
TGATAACCCCAATAGTGAAGAGGTGTCAAAACACCGTGACAGACTGAAGG
ATTATCTGTGGCTTGCTGAAGATGGGATGAAAATGCAGGGATATAATGGT
TCTCAACTTTGGGACACCGCGTTCACCAGTCAAGCTTTCATGACGGCCGG
GGAAGAAATCCTACGTCCTTTTGTCAGAACATTGTCGCTAGCTCATGATT
ATATCAGAATCACGCAAGTCAGAGAGGATGTTCCCAATCGCGAAAGATAT
TACCGACATCCAAGCAAAGGGGCATGGCCTTTCAGCACAAGAGATCATGG
TTGGCCAATTGCAGATTGCACTGGAGAAGGGCTAGCAGCGTCGTTACTCT
TGCAAGGTGAGGAAGGGTCGTGGATTCCAAAAACTCAGTGCATATCGTTA
GAACGACTTGCAGACGCTGCCGAAATGATTCTCAGTTACCAAAATGAAGA
TGGTGGTTGGGCTACCTATGAGCTTACTCGTGGTCCCAGATGGCTCGAGT
ATCTAAATCCGTCTGAAGTGTTCGGAGATATCATGATTGATTACTCCTAT
GTTGAATGCTCTGCTTCAGCCATGAAAGGTTTGGGGGAGTTTCGGAAAGC
TTACCCTAACCATCCGTTGAGGCCCAGAATCGACAGATCAGTCGAAAGAG
GAATCAAGTTTATTGAGTCCATTCAAAGGGAAGATGGCTCATGGTATGGA
AGCTGGGGGGTTTGCTTTCTGTATGCGTTCTGGTTTGCAATGGACGCGTA
CTCTTCTGTTGGGCTCACACCTGACTCGAGTAGTTCTATGCGACGGGCAT
GTGAATTTATTTTGAGCAAACAGAAGGAAGACGGTAGCTGGGGAGAGAGC
TATCTCAGTTCTGAACAATTGGTCTATGTGCAGTCAAAACATGGGCTTGT
TGTTAGTACGGGTTGGGCGCTAGTGGCATTAAGCATGGCGAGATGGTTTA
ACAAGGAGCCACTGGAGAAGGCTGCGCAGTTTCTCATTCGCTCGCAAGAA
GAAAATGGAGACTGGCCACAGCAAAACATCTGTGGTGTCTTTAACAAGAA
CTGTATGATATCATATTCCCAATACCGTAACATCTTTCCAATTTGGGCTC
TTTCTGAATATCGAAGGTATTGTACATCATGA