Gchil5632.t1 (polypeptide) Gracilaria chilensis NLEC103_M9 male

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A2V3J3X5_9FLOR (Pullulanase n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J3X5_9FLOR)

HSP 1 Score: 1948 bits (5046), Expect = 0.000e+0
Identity = 945/1495 (63.21%), Postives = 1146/1495 (76.66%), Query Frame = 0

Query:    5 APAHLVVHYHRPYHDYYNWCLTLGDNVYAQCIPDAFTAYGALFRISLPSQSQLLLQPTHPALIDSTPRRVDLSSLHSTGRDKHVFLLCDHPHAVSSPLLFLTGQFSRFVFVTADWGEEWLVLYAGNTKRDLFPATCHYRVPAHPSLFVLDTSALPKEISFTLQKTHTYTAPSHSQPSFPSFEAPFPHHIPLSSTPFR---TPFDPLSVLVEQTSRSAYLSLIVSTMAPVTRPNPSPVIAPSKHLILFYYRLDRSTPIVSIMLTAATNLQSVSLPSPYHTARAFSIDITQLLQLKNSKSKSSQLSIVPIRTSVSSTIQEPPLTWKPSLGLRIIYAQSYPRLLTPSDVDISIVYHRYDPRWTDWELNIWTNATEKYPALSTKLTPRRPAVPGQIVFHLIGHIFPFKTQVYAEPVRMQLHPAQATIDQFGHTVMTEPHRDACRRDVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFMAKRWFRVRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPVSVTKDWTDFIVDRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLE-EEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSECYVSQFEMRLASIADVHCSFHGDDPLSRYAVGLEPMPTSEEQAFKNQSIFQRIQF 1495
             P HLVVHYHRP H Y++W LTL +  YAQ + +A T+YGALFRI  P  + + L PTHP L+D+ PR+ DL+SL +  R+ HVFLL  HP AV SP LF T    R   ++      WL++YA +TKRD+FPA+ HYR   HP+LF+LDTS L ++I  T+Q  +   AP+ +Q S+P+F AP+P H+ LS+ P +   T  D  + +  Q  +  +LS  V    P    +P       KHL+LFYYRLDRSTPIVS+ ++A+  L++V LPS YHT++AF+IDI+ L +   S S  SQLSI P+R++    I E  LTW+PSLGLR+IYAQS+P LLTP+  D+++VYHRYD  W+DWEL++W+   + YPA+S KLTP RP  PGQ+VFHL+G++FP++ QV+ EPVRM LHP  AT D+FG TV+ EP+RDACRRDV+R+WTSA+ P+Q++H VQGD +I   PP+P  F A+R FR+RYRRF PSDY GWDLWTWD TDP SHR+ L PV+ T+DW DF+VDRA YGS K I +LPRRGG AWLE+D   R W+ S+LE       K  E      D H  D+ PTF ITQ+T  VLR LD+ + MLDAYVDS++S+VVS PVPL W+ P +    Q  +++ IK+   PVQ +   G  + + + GKSLR  K++Q++  KT  +F+ SV HF+EDFLVENV+V V  F +V LHWE H+NED+Y YEGSLGW YS+ +C+FRCFAP A  VSVV+Y + TG  GR VVPMRRIPQGCWK  + +DLKG YYKLLAEG  KRLFPG+EVIDPYSRCNT H GRGL+YG ++T V+ RP+++ +  I+YELHIRD+TI+  SGIR  GKFLGL ERGT + P      + K LT W QE M GV  +F +L+KFS+GLDHI QMGVTA+QIMPIQDFDN+E D+ +YRWGYMPVHFNSPDGWYASSTHT ARV EFK LVSA HKAGL+VIMDVVYNHTAEDSNELNL+ARFSFNGLAPRYYYRTC NTP+AH G+ TCG R   +P CGECYSNGSGCGNEFRSESPMGRKFI+DSLKFWA EYGVDGFRFDLLGLID+ETI +A  ELK+I P I+VYGEPWIGGLSP+K TDKGSQRSKGF VFNDTFRNA+RGSPFDTEETFIMDGGRL EVKGGIIGSVD F DSPLES+NYVECHDNRTLWDHL +Y RSRTD IKF++ED+RRMH+LAAVLVLTSQG+PFIQAGQEMCRTKF DENSYES DEINMIRW TK+ EWSTVQYYRGLILLRR+HPEIFC+E ADEIH+K+ FYEDCGL VP++CI YRIKGNPE+L R+LQEE   DV  ++D   EE  RWSEVV+L NATPL V FELPG++ D +WMQ V +SAAGTR + GP +GSV VSGRSAAVLRRAS +E  V Q EMRL+SI+D + SFHGDDPL+RY VGL+P PT E+ + +N  I QR++F
Sbjct:    7 TPTHLVVHYHRPNHHYHSWTLTLPNATYAQPVQNALTSYGALFRIPFPLPASVTLHPTHPKLVDAPPRQCDLTSLTAVHRELHVFLLEAHPQAVRSPSLFQTHPNPRLFAISVPDPHAWLIVYARDTKRDVFPASAHYRQRNHPALFLLDTSTLARQIRLTIQHHYHRPAPATAQLSYPAFRAPYPKHVMLSAHPLKAQQTDQDLFAAITTQALQCRFLSRQVQ--GPTVLDSPLIRTPKYKHLVLFYYRLDRSTPIVSLTVSASVKLEAVPLPSSYHTSKAFTIDISALPE---SHSSHSQLSITPVRSNSGVVIHENKLTWRPSLGLRVIYAQSFPHLLTPAQTDLTLVYHRYDSEWSDWELHVWSKPQDGYPAVSAKLTPTRPVKPGQVVFHLVGYVFPYQAQVFVEPVRMALHPGHATTDEFGITVVGEPYRDACRRDVIRKWTSAQPPSQVIHLVQGDSRIYAHPPDPDTFNAQRRFRLRYRRFLPSDYEGWDLWTWDQTDPESHRIPLTPVAETRDWADFVVDRADYGSGKIISILPRRGGDAWLEQDEFVRQWSLSMLESLDTSIVKHHEPLKQRADDH--DDLPTFLITQETKLVLRHLDDARTMLDAYVDSDNSIVVSAPVPLQWLMPPQLTQSQPNEDMVIKIAQRPVQGLLDVGKEA-DDIEGKSLRFLKAKQVTPIKTGYVFDKSVAHFEEDFLVENVVVSVPGFVSVALHWETHENEDKYLYEGSLGWEYSSAKCIFRCFAPIASLVSVVLYDEPTGDAGRTVVPMRRIPQGCWKVTIIEDLKGRYYKLLAEGGNKRLFPGVEVIDPYSRCNTGHTGRGLVYGIDSTEVYPRPNVKPSAAIVYELHIRDITIDKCSGIRQRGKFLGLTERGTGL-PNKVSKSESKVLTQWPQERMRGVNDEFSLLDKFSTGLDHIVQMGVTAVQIMPIQDFDNDESDDGSYRWGYMPVHFNSPDGWYASSTHTVARVVEFKHLVSALHKAGLKVIMDVVYNHTAEDSNELNLDARFSFNGLAPRYYYRTCGNTPIAHTGDRTCGVREHPKPHCGECYSNGSGCGNEFRSESPMGRKFIIDSLKFWAMEYGVDGFRFDLLGLIDLETIHRAATELKSIHPHILVYGEPWIGGLSPIKITDKGSQRSKGFGVFNDTFRNAVRGSPFDTEETFIMDGGRLPEVKGGIIGSVDLFTDSPLESLNYVECHDNRTLWDHLTYYTRSRTDNIKFNEEDLRRMHRLAAVLVLTSQGVPFIQAGQEMCRTKFDDENSYESSDEINMIRWMTKLNEWSTVQYYRGLILLRRSHPEIFCLETADEIHKKVAFYEDCGLCVPERCIAYRIKGNPERLFRQLQEEEP-DV--DRDFHREESLRWSEVVVLLNATPLHVTFELPGRKTDTLWMQTVDSSAAGTRIIQGPHVGSVTVSGRSAAVLRRASPTEQEVGQLEMRLSSISDAYSSFHGDDPLTRYTVGLDPRPTVEDVSQRNDLISQRLEF 1489          

BLAST of Gchil5632.t1 vs. uniprot
Match: R7Q3N6_CHOCR (Pullulanase, Pul1 n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7Q3N6_CHOCR)

HSP 1 Score: 1424 bits (3685), Expect = 0.000e+0
Identity = 751/1535 (48.93%), Postives = 969/1535 (63.13%), Query Frame = 0

Query:    9 LVVHYHRPYHDYYNWCLTLGDNVYAQCIP---------DAFTAYGALFRISLPSQSQLLLQ--PTHPALIDSTPRRVDLSSLHSTGRDKHVFLLCDHPHAVSSPLLFLTGQFSRFVFVTADWGEE----WLVLYAGNTKRDLFPATCHYRVPAHPSLFVLDTSALP-KEISFTLQKTHT----YTAPS--HSQPSFPSFEAP-----------FPHHIPLSSTPFRTPFDPLSVLVEQTSRSAYLSLIVSTMAPVTRPNPSPVIAP--SKHLILFYYRLDRSTPIVSIMLTAATNLQSVSLPSPYHTAR----AFSIDITQLLQLKNSKSKSSQLSIVPIRTSVSSTIQEPPLTWKPSLGLRIIYAQSYPRLLTPSDVDISIVYHRYDP--RWTDWELNIWTNATEKYPALSTKLTPRRPAVPGQIVFHLIGHIFPFKTQVYAEPVRMQLHPAQATIDQFGHTVMTEPHRDACRRDVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFMAKRWFRVRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPV--SVTKDWTDFIVDRASYGSAKNICLLPRRGGKAWLERDGVERVW------------NASLLEDAHGMEAKGT---ESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTT----TKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSECYVSQFEMRLASIADVHCSFHGDDPLSRYAVGLEPMPTSEE 1481
            LV+HYHR   D++ W L L  +  +   P            T++G  FRI L + S   +   PTH  L    P R+ L+   +T  D H+F++         P  F     +   FV     +     W+VL  G TK +LFP +  +R P  P L++LD + L    +S      H+       PS  H+    P   A               H  L++TP      PL +L  Q  R   LS+I S  AP T P P  V  P   K L ++  +L R+   V + L    + +   LP    T       F +++ +L   K SK     L IVP+      T       W P +GL ++ A+S+  L+    V++S++YHR+     W DWEL++WT A +++PA ST L P R  +PG   F+  G++FP    V AEPVRM L P +   ++ G     EP+ DA RRDVVREW S  APA +LHF+QG  ++    P        R FR+RYRR+ P DY+ WDLWTWD +DP ++R+A+AP+  S+T+ W DF +DRA YG+  +I L+PR+  + W ERD   RVW            + +L+ DA   E  G    + DF           P FTI Q ++ +LR L+EVK M+ AYVDSE S+++++PVP+ W+ P  +   Q   +  +++C               +  TG  L  +K  +++ T+ RL F    V F+EDFL+ENV V V  F +V L W  H++ D+Y Y G+LGW Y   +C F+CFAP AD + V++Y    G EGR  VPMRRIP+GCWKAIV +DLKG YYKL A G+  RLFPG+EVIDPYSRCNT+H GRGLI+G E T +   P + A+E IIYELH+RD+TI+ +S +   GKFLG+ ERGTKM   +    T     KPLTPW+QE++P ++     L+KFSS LDHI QMGV  IQI+PIQDFDN+E+D+ +Y WGYMPVHFNSPDGWYASST + ARV EFK+LV A HKAGL+VIMDVVYNHTAEDSNE NL+ARFSFNGLAPRYYYRTC NTPVA+ G+STC  R P EPRCG CYSNGSGCGNE RSESPMGRKFI+DSLK+W +EY VDGFRFDLLGLIDV+T++ A  EL+ I+P++++YGEPW GGLSP++ T KG QRS+GF VFN+TFR+AIRGSPF TEETF+MDG RL E+KGGIIGSVD F D P E++NYVECHDN TLWDH   YIRSRTD I F++ D+RRMH+LAA +V TSQGIPF+Q GQEMCRTKF  ENSYESPD+INM+RW  K  EW+TVQYYRGLILLRRAHPE+FC   ADEIHE +VFYED GL VP++CIGYRI GN  KL +RL     +     + L EE  +W+EVV+L N TP +V+FELP  E D +W+++V A+ +GTR L  P IG++EV GRSA VLRRAS  E    Q  +RL SI+D + SFHGDDP+++YAVGL    + +E
Sbjct:    9 LVIHYHRTQGDHHRWDLFLSASQSSPFTPIKVISPVCESGVTSFGTAFRIPLSALSSCSISVLPTHAILGTDNPPRL-LTVPTTTSSDLHIFIVQGDSAVYGDPFAFELPSIASPRFVGLSLSDSSSNPWIVLLNGGTKCELFPVSSPFRDPQAPPLYLLDQAVLAGSAVSIATVVEHSDAVCIAGPSNKHATLDLPVTSASRIQLRRDDNGNLQLHPDLTTTPL-----PL-LLAAQADRCPSLSIIRS--APSTIPPPECVHVPPKGKPLRVYLKQLGRNVYNVKVDLLVTDSSRMTLLPQRLETLDDGRPIFDVNLVKLSPEKISKG----LRIVPVVGGKIGTA----FLWTPEMGLDVVLAESFSELIKIEHVNLSVMYHRFGDFEDWNDWELHVWTEANDRHPAYSTMLKPERSILPGIARFNFDGYLFPCGAVVRAEPVRMALFPGRMLTNEHGEQYQGEPYTDALRRDVVREWVSGSAPAAVLHFLQGHSEVLTDAPLAADMRYGRKFRLRYRRYLPEDYDEWDLWTWDESDPENNRIAVAPMPDSITRAWVDFEIDRARYGAGASISLVPRKNREMWSERDEPTRVWRNDMCNRTSRTLDVALVTDAPSTEIGGECPFQEDF---------SVPMFTIVQGSSLILRKLEEVKSMMTAYVDSEESIIIASPVPVAWVSPPNRGRSQAISDTVVRVCSAATNGFRSADGHIEDDTTGIVLPIRKFHEVAPTQFRLTFNEKAVRFEEDFLIENVSVTVPGFDSVRLTWRIHEDWDKYHYTGTLGWEYEVTKCSFKCFAPTADNMYVILYNSPHGKEGRRAVPMRRIPEGCWKAIVEEDLKGKYYKLRAGGENPRLFPGVEVIDPYSRCNTSHTGRGLIFGFEKTAIQPGPCIPASETIIYELHVRDLTIDENSSVIKRGKFLGVTERGTKMKAVSSGSGTGDTSAKPLTPWKQELIPTLEVGMQSLDKFSSALDHIVQMGVNTIQILPIQDFDNDENDDSSYGWGYMPVHFNSPDGWYASSTSSVARVTEFKQLVDAVHKAGLKVIMDVVYNHTAEDSNEYNLDARFSFNGLAPRYYYRTCGNTPVAYTGDSTCAMRKPDEPRCGACYSNGSGCGNELRSESPMGRKFIIDSLKYWVTEYKVDGFRFDLLGLIDVDTLEMAGSELRQINPNVMIYGEPWAGGLSPIRITAKGCQRSRGFGVFNNTFRDAIRGSPFGTEETFVMDGSRLTEIKGGIIGSVDDFCDFPTETVNYVECHDNYTLWDHFRFYIRSRTDDIVFTENDMRRMHRLAAAIVFTSQGIPFMQLGQEMCRTKFDVENSYESPDKINMVRWEAKQSEWTTVQYYRGLILLRRAHPELFCKSTADEIHEGLVFYEDLGLAVPERCIGYRILGNSAKLYQRLLTTNPD--ADYEALREESMKWTEVVVLLNPTPSQVVFELPDHERDAIWVEVVDATHSGTRGLRMPMIGAIEVQGRSACVLRRASEREEVDLQLRLRLNSISDCYSSFHGDDPITKYAVGLSSQRSEKE 1515          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A7S3E8R2_9RHOD (Pullulanase n=4 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E8R2_9RHOD)

HSP 1 Score: 914 bits (2362), Expect = 1.150e-305
Identity = 500/1133 (44.13%), Postives = 652/1133 (57.55%), Query Frame = 0

Query:  324 WKPSLGLRIIYAQSYPRLLTPSDVDISIVYHRYD-PRWTDWELNIWTNATEKYPALSTKLTPRRPAV----PGQIVFHLIGHIFPFKTQVYAEPVRMQLHPAQATIDQFGHTVMTEPHRDACRRDVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFMAKRWFRVRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPVSVTKDWTD-----FIVDRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEG---RVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAH--AGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGN-PEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSE 1440
            W+P  G  +   +     +T       I YHR   P  + W+L +  +  +K    S  + P  PA+    PG  +F L    FP  +++   PV+      +  ++  G           C R+V  +    E     +H  QG   +    P+      +R+F V YRR+   D  GWD+WTWD  D  S  VA  PV    DW +     F VDR+ YGS + I L+ R+GG+ W  +D  +R+W A           +G   +F+            +   Q    ++R L++    L AYV+++ ++ + + + L W  P                    +    K+ +L      GK+++  K E+ S+    +  +      DEDF VE+  +    F    L W R+ N D YFYEG LG  +   +C FR FAP AD VSVVIY  A G  G   R V PMRRIPQGCWKA +  +LKG YYKLLAEG  KRL+PG+EVIDPYSRCNTAH   GRGLIY  + T + DRP    +  +IYELH+RD TI+ +SG+   GKF+GL ERGT               T W+++    V        K S+ L+HI +MGV  +QIMPIQDFDN+E+ +  YRWGYMPVHFNSPDGWYASST   +RV EFKKLV   H  G++V+MDVVYNHT+ED NE NLEARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFI+DSLKFW  EY +DGFRFDL+GLID ET+ QA  ELK ID  I++YGEPW GG SP+  T KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD  +HY   RTD+I F+D D  RM KLAA+++ TSQG+PFIQ GQEMCRTK G ENSYESPD +NM++W  K E    V+YY+GLI +R AH  +F M  A +I + ++F+E+ GL  P++CI +RI  + PE   +                     RWS+VV+L N TPL+  F LP  E D +W  +    AAGT T+  P    + V GRS  VLRR S  E
Sbjct:  315 WQPEFGYHVFLLEGMEGPVTFEPRPRQITYHRLGVPVDSSWQLEVTCDEWKKLAGSSQAIVPV-PALSEKQPG--LFELSDFWFPPSSKIMIRPVK-----TREDLETDGPESTKLYDMTDCWREVPEDRLEGE-----MHLSQGYLGLFQDEPDQKVLNRERYFYVEYRRYD-DDGAGWDIWTWDNDDDFSDPVAAQPV----DWENAAYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKA-----------EGRRPNFVDP----------YIFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWFHPN-------------------IDDSSKKSSLIIKSANGKAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGTST-------------TVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTKGGVENSYESPDSVNMVQWKWKQERRRCVEYYKGLIKMRLAHQHLFAMTYASDIRKNLIFFEELGLQPPERCIAFRISSDHPEGPSKA--------------------RWSQVVVLLNPTPLEQRFWLPN-EPDFLWGPVADDVAAGTDTIGAPVQDQISVRGRSGCVLRRISRVE 1347          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A5J4YVH4_PORPP (Pullulanase n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YVH4_PORPP)

HSP 1 Score: 868 bits (2244), Expect = 5.170e-283
Identity = 489/1119 (43.70%), Postives = 629/1119 (56.21%), Query Frame = 0

Query:  391 GQIVFHLIGHIFPFKTQVYAEPVRMQLHPAQATIDQFGHTVMTEPHRDACRRDVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFMAKRWFRVRYRRFSPSDY---NGWDLWTWDPTDPASHRVALAPVS-------------------VTKDWTDFIV---DRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCK--RLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKM----------------VPTTTKHPQR--------------KPLTPWEQEIMPGVQKDFFVLEKF---------SSGLDHIAQMGVTAIQIMPIQDFDNNED--DEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGT-RTLSGPFIGSVEVSGRSAAVLRRASSSE 1440
            G+ +F      FP    +Y +PV + L      +                  D V+ W        I H VQG   +          +A     + Y R   S       WDLW WD  D     +   P                     V  D   F+V    RA  G    I LLPR GG AW +R      W+ +     H  +    E             FP          V    D V  M  + +D ++ V +ST +P  W  P +  A               V+ + K  +  +  V   +        +     R  FE   V FDEDF VE   V V   H   L W  ++NED Y+Y+G+LGW YS  + VFRCFAP+A ++SVV+Y+  +G +GR +V MRRI  G WKA +  DLKG YYKLLA       R FPG+EVIDPYSRCNT+H GRGLI+G +TTPV DRP++     IIYE+H+RDMTI+  SGI   GKFLGL ERG+ +                V T T+  ++              K     E  +  G Q D   L            S+ LDHI +MGVTA+QI+P+QDFDN+E   D   Y WGYMPVHFNSPDGWYA+ T +AARV EFK+LVSA H  GL+VIMDVVYNHTAED+NE NLEARFSFNGLAPRYYYR C NTP++    +TC  R  HE RCG C+SNGSGCGNEFRSE+PMGRKF++DSL++W SEY VDGFRFDLL L+D ET+     E++ IDP+I++YGEPW GG++P+  T+KG+QR +GF+VFNDTFR+A+RGS +  +ETF+MDGGR+ EVK GI+GS+D FADSPLESINY+ECHDNRTL+D   HY+ +R D I ++D D  RM +L A++VLTSQG+PFI AGQEM RTK G ENSY SPD+INM+RW+ K E    V+Y++ LI LRR HP++F M NA E+ E+I F+ED  L VP++CI Y ++G  E         R + +  E+    E Q+WS+VV+L N TP+   F LPG      W+ +V    A   R +  P +  V V GRS  +LR A+  +
Sbjct:  722 GRYLFDTSVAHFPQGRAIYVQPVLLDLDTGTPLV-----------------ADCVKLWPERTHGPDISHAVQGARDVXXXXXXXXDALATPNVGIMYLRHGVSTRAHARQWDLWVWDAEDETVAALLPDPADKEPSSSSDGFGFGQPHMDVVNIDGLSFVVFSIPRACLGCGNRIKLLPRHGGAAWTDRGAHTVTWDCTR----HPRDRMLLEG------------FPEL--------VAELFDAVGDMTASVID-DNIVEISTSLPASWFAPEQDPAN--------------VKAVFKGASSGHQNVLDLNKVLLSMTSVDAWTMRATFEPRAVRFDEDFPVELYSVSVLNMHDARLRWPVYENEDAYYYDGTLGWEYSPSETVFRCFAPSAAELSVVLYSAPSGQQGRELVSMRRIMGGVWKARIQGDLKGRYYKLLALSTPDDTRRFPGVEVIDPYSRCNTSHTGRGLIFGRDTTPVTDRPNVPPQNAIIYEIHVRDMTIDQQSGILMRGKFLGLTERGSMLQEGEQEPSAGQNCETKVGTVTEQARKLGVADSVAARASPSKRAQKAENGVSQGEQLDHLPLPDIIDDVEITPLSTALDHIIEMGVTAVQILPVQDFDNDESSTDGNQYWWGYMPVHFNSPDGWYATDTTSAARVSEFKQLVSALHDVGLKVIMDVVYNHTAEDANEFNLEARFSFNGLAPRYYYRNCGNTPLSVGDHNTCANRGKHEKRCGTCFSNGSGCGNEFRSEAPMGRKFLLDSLRYWVSEYKVDGFRFDLLALVDTETVAMLAREMQKIDPNIMLYGEPWTGGMTPIAPTEKGTQRGRGFSVFNDTFRDALRGSTWSVDETFLMDGGRIDEVKRGILGSIDVFADSPLESINYIECHDNRTLYDQFQHYVANRQDDITYTDADYERMARLGALIVLTSQGVPFIHAGQEMMRTKQGVENSYCSPDDINMVRWAWKRERLMCVRYHQHLIRLRRTHPQLFSMTNAAEVRERITFFEDLDLIVPERCIAYHMQGYKEGEYAHGY--RIDSLSSEERRRIE-QQWSQVVVLLNPTPMGTDFGLPGAAHQQAWVCMVDDEFASCERAVRAPVMIQVHVPGRSGMLLRAAAPDD 1781          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A7S3E758_9RHOD (Hypothetical protein n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E758_9RHOD)

HSP 1 Score: 791 bits (2042), Expect = 2.660e-264
Identity = 430/958 (44.89%), Postives = 557/958 (58.14%), Query Frame = 0

Query:  324 WKPSLGLRIIYAQSYPRLLTPSDVDISIVYHRYD-PRWTDWELNIWTNATEKYPALSTKLTPRRPAV----PGQIVFHLIGHIFPFKTQVYAEPVRMQLHPAQATIDQFGHTVMTEPHRDACRRDVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFMAKRWFRVRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPVSVTKDWTD-----FIVDRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEG---RVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAH--AGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTK 1266
            W+P  G  +   +     +T       I YHR   P  + W+L +  +  +K    S  + P  PA+    PG  +F L    FP  +++   PV+      +  ++  G           C R+V  +    E     +H  QG   +    P+      +R+F V YRR+   D  GWD+WTWD  D  S  VA  PV    DW +     F VDR+ YGS + I L+ R+GG+ W  +D  +R+W A           +G   +F+            +   Q    ++R L++    L AYV+++ ++ + + + L W  P                    +    K+ +L      GK+++  K E+ S+    +  +      DEDF VE+  +    F    L W R+ N D YFYEG LG  +   +C FR FAP AD VSVVIY  A G  G   R V PMRRIPQGCWKA +  +LKG YYKLLAEG  KRL+PG+EVIDPYSRCNTAH   GRGLIY  + T + DRP    +  +IYELH+RD TI+ +SG+   GKF+GL ERGT               T W+++    V        K S+ L+HI +MGV  +QIMPIQDFDN+E+ +  YRWGYMPVHFNSPDGWYASST   +RV EFKKLV   H  G++V+MDVVYNHT+ED NE NLEARFSFNGLAPRYYYR C + PVA +G +TC      EP CG CYSNGSGCGNEFRS++PMGRKFI+DSLKFW  EY +DGFRFDL+GLID ET+ QA  ELK ID  I++YGEPW GG SP+  T KG QR +GF VFND+ R+A+RG  +  +ETF+MDGG   +VK GI+GS++ FA  P E+INY ECHDNRTLWD  +HY   RTD+I F+D D  RM KLAA+++ TSQG+PFIQ GQEMCRTK
Sbjct:   28 WQPEFGYHVFLLEGMEGPVTFEPRPRQITYHRLGVPVDSSWQLEVTCDEWKKLAGSSQAIVPV-PALSEKQPG--LFELSDFWFPPSSKIMIRPVK-----TREDLETDGPESTKLYDMTDCWREVPEDRLEGE-----MHLSQGYLGLFQDEPDQKVLNRERYFYVEYRRYD-DDGAGWDIWTWDNDDDFSDPVAAQPV----DWENAAYPTFRVDRSLYGSGREILLIGRQGGEHWTAKDEPQRMWKA-----------EGRRPNFVDP----------YIFLQGHPHLVRRLEDAIGNLSAYVETDGTISLHSSMDLKWFHPN-------------------IDDSSKKSSLIIKSANGKAMKIVKVERKSSVLRVIHVDIQSQLLDEDFRVEDFTISARNFSPEVLTWRRYDNFDEYFYEGDLGLEFGIAECTFRTFAPGADHVSVVIYDTAVGPNGGGGRKVHPMRRIPQGCWKANIKANLKGKYYKLLAEGSDKRLYPGVEVIDPYSRCNTAHHTEGRGLIYPMDNTRIADRPKFPTSASVIYELHLRDSTIDPASGVNKKGKFMGLTERGTST-------------TVWDEKTGQNV--------KISTALEHILEMGVNTVQIMPIQDFDNDENSQEEYRWGYMPVHFNSPDGWYASSTSNFSRVVEFKKLVDCFHSNGIKVVMDVVYNHTSEDPNEFNLEARFSFNGLAPRYYYRNCVSHPVAASGHNTCAWHGMDEPHCGTCYSNGSGCGNEFRSDAPMGRKFIIDSLKFWVEEYKIDGFRFDLMGLIDQETMTQATAELKKIDDGILIYGEPWTGGPSPIVATVKGFQRDRGFGVFNDSLRDALRGGNWGIQETFLMDGGEAEKVKRGIMGSIEDFASQPTETINYAECHDNRTLWDQFSHYRELRTDSIVFTDSDFIRMGKLAALIIFTSQGLPFIQIGQEMCRTK 906          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A1X6PAN7_PORUM (Aamy domain-containing protein n=1 Tax=Porphyra umbilicalis TaxID=2786 RepID=A0A1X6PAN7_PORUM)

HSP 1 Score: 759 bits (1961), Expect = 3.600e-249
Identity = 463/1143 (40.51%), Postives = 610/1143 (53.37%), Query Frame = 0

Query:  443 DVVREWTSAEAPAQILHFVQGDPKIRVRPPEPHQFM------------AKRWFRVRYRRF--SPSDYNGWDLWTWDPTDPASH--RVALAPVSVTK-DWTDFIVDRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVH---FDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTE----TTPVHDRPSLRAAE-IIIYELHIRDMTIENSSGI-RNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFV-----------LEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFA-YRWGYMPVHFNSPDGWYASSTHT-AARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIR-----SRTDTIKFSDEDV----------------------------------------------------RRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSECYVSQFEMRLASIADVHCSFHGDDPLSRYAVGLEPMPTSEEQAFKNQSI 1489
            DV R WT           VQG P I     E  +F+            A RWF VRYRRF      Y GW LW  D     +    VA+ PVSV   +   F+VDR  +G    + L+PR   +A ++  G    W+    + A  + A  T  D +     +    P   + Q    +L SL      +   VDS   V V+T  PL W+  T  A      ++       P +L  + G      VT         E+ S T+  L    +V       ED+LVE V+        + L W RH N D Y+Y G++G   +A + VFR FAP AD V+VV+Y        R  VPMRRIPQGCWKAIV   L G YYKLLA G    LFPG+EVIDPYSRCNT H GRGLI   +       V  RP +   + ++I+ELH+RD T++ +SG+  + GK+LGL +R T +VP        K +     ++  G                      S+ L H+ +MG TA+Q++P+QDFDNNED+    Y WGYMPVHF SPDGWYAS + T + RV+E K+LVSA H+AGLRVI+DVV+NHTAED NE NL+ARFSFNG+APRYYYRTC NTP++ +G  TC       P CG CYSNGSGCGNE RSE+PM RKFI+D L++WA+EYG+DGFRFDL+GL+DV T+  A   LKAID  I++YGEPW GG++PM  T KG+QR +GF VFNDTFR+A+RGSPF     F++DG  + EVK GI+GSVD FAD+P E INYV CHDNRTL+D L    R     +  D     +E V                                                    RR   LA  +VLT+QG+PF+Q GQE  RTK GD NSY SPD +N +RW+ K    + V++  GL+ LRR HPEIF +  A  + E+++F+E  G+ VP  C+ YR+ G    ++ R     +          E+  RW+ V++L N       F LP  EA+ +W+ +V+   AG   +  P    + V+  S  VLRR S++E   +  + RLA I+D        D  SRYAVGLE    + E A   +++
Sbjct:   11 DVARSWTILPGVVPRRQIVQGHPTIY---REASEFLGDGGGDTTVCSAASRWFAVRYRRFVGEADAYAGWVLWGCDDDAVGASVGGVAIEPVSVDPVEGALFLVDRGRFGDGSRLRLVPRHAERA-VDDAGATVTWDVDAADAAFWIPA--TVVDALVAGGGDTPP-PALLLVQGHGSLLTSLASEPSPVVVSVDSPWDVAVTTFCPLEWLDGTAPAKPW---DLAASPSTRP-KLTLRHGQAELPWVTW--------ERPSPTEVVLRLSEAVADGRGLHEDYLVEEVVARATGIPTLALQWRRHANVDAYYYPGAMGCMCTASETVFRVFAPTADGVAVVLYDGPGDDASRREVPMRRIPQGCWKAIVPSSLGGTYYKLLASGADTLLFPGVEVIDPYSRCNTHHTGRGLILAPDDPAVVATVAPRPPVGPDDAVVIWELHLRDATVDPASGVVGHRGKYLGLTQRDTVLVPGAPFAADPKGMV----DVSAGADAADTAKPAPAANGEADAPPVSTALAHLVEMGATAVQLLPLQDFDNNEDEAVGEYAWGYMPVHFFSPDGWYASGSRTDSTRVRELKELVSAMHEAGLRVILDVVFNHTAEDVNERNLDARFSFNGVAPRYYYRTCGNTPLSASGHRTCALTLAGAPTCGGCYSNGSGCGNEMRSEAPMARKFIIDCLRYWATEYGMDGFRFDLMGLVDVPTLTAASTALKAIDDGILLYGEPWTGGMTPMAPTYKGTQRGRGFGVFNDTFRDALRGSPFHVSSCFVLDGVCVDEVKRGIMGSVDDFADAPTEVINYVACHDNRTLYDQLWEIYRGGGEDTGADVAPPEEEPVGASAVKDIGPAPDATHLSPDAATAVAXXXXXXXXXXXXXXXXXXXXXVPALLRRCAALATAIVLTAQGVPFLQLGQEFFRTKGGDHNSYISPDAVNAVRWARKAAHAAAVRFVAGLVALRRGHPEIFALPTAGLVRERLLFFETMGVPVPAGCVAYRVDGCAADVVARAAAAGASAATAN----EQAARWTAVLVLINPCRTAQAFALP--EAEQLWVPVVAGGHAGLSPVGAPVRRQLGVAPTSLTVLRRCSAAEADAAAVDDRLAVISDPGVVPADGDCTSRYAVGLERERGAAEAAVMEENV 1124          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A7V5M1D9_9BACT (Aamy domain-containing protein n=1 Tax=bacterium TaxID=1869227 RepID=A0A7V5M1D9_9BACT)

HSP 1 Score: 736 bits (1899), Expect = 1.040e-244
Identity = 400/789 (50.70%), Postives = 512/789 (64.89%), Query Frame = 0

Query:  703 DEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGI--RNGGKFLGLAERGTKMVPTTTKHP-----------QRKPLTP--WEQEIMPG-VQKDFF---VLEKF---------SSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDT-IKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVS---ASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSECYVSQFEMRLASIADVHCS 1459
            DEDF VE V+V +  F      W R++N D Y+Y G+LGW Y+     FR FAP AD V + +Y +  G  GR   PMRRIPQG WK +V  DL+G YY  LAEG  KRLFPG+EVIDPYSRCNTAH GRGLI+G + T V DRP +     IIYE+H+RD TI+  SG+  +  GK+LGLA++GT++     +              R   TP  +E+++ P  V++D +   VL +          S+ LDH+  +GV A+QIMP+QDFDN+E +   YRWGYMPVHF SPDGWYAS T  A+RV+E K+L+   H+AGL+VI+D+V NHTAED NE NLEARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L +WA+EY VDGFRFDL+GL+D ET++ A  +L A+DP+I+VYGEPW  G +P+  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SPLE INYVECHDNRTLWDHL + IR R D  I ++  DV R+ KLAAV++LTSQGIPF+ +GQEM R+KFG ENSY+S D+IN +RW  K++ +  V Y RGL+ LRRA P+IF M +A ++   I FYE  GL VP++C+ YRI      +    Q E +        +E++P  W  V +LFN TP+  +F LP +  D +   IV+   A      +L    IG   V GRSA VLR  + SE   +  E RL +I++ + S
Sbjct:   19 DEDFPVEEVMVHLKGFSPSRGAWRRYENVDEYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATPDAFEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDRSTCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRI------IADAGQSETNA-------IEDDP--WRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 777          

BLAST of Gchil5632.t1 vs. uniprot
Match: M1VKA6_CYAM1 (Pullulanase n=1 Tax=Cyanidioschyzon merolae (strain 10D) TaxID=280699 RepID=M1VKA6_CYAM1)

HSP 1 Score: 735 bits (1897), Expect = 2.280e-232
Identity = 400/789 (50.70%), Postives = 512/789 (64.89%), Query Frame = 0

Query:  703 DEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGI--RNGGKFLGLAERGTKMVPTTTKHP-----------QRKPLTP--WEQEIMPG-VQKDFF---VLEKF---------SSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDT-IKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYEDCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVS---ASAAGTRTLSGPFIGSVEVSGRSAAVLRRASSSECYVSQFEMRLASIADVHCS 1459
            DEDF VE V+V +  F      W R++N D Y+Y G+LGW Y+     FR FAP AD V + +Y +  G  GR   PMRRIPQG WK +V  DL+G YY  LAEG  KRLFPG+EVIDPYSRCNTAH GRGLI+G + T V DRP +     IIYE+H+RD TI+  SG+  +  GK+LGLA++GT++     +              R   TP  +E+++ P  V++D +   VL +          S+ LDH+  +GV A+QIMP+QDFDN+E +   YRWGYMPVHF SPDGWYAS T  A+RV+E K+L+   H+AGL+VI+D+V NHTAED NE NLEARFSFNGLAPRYYYR     P    G S              CY+NGSGCGNEFRSESPMGRKFI+D L +WA+EY VDGFRFDL+GL+D ET++ A  +L A+DP+I+VYGEPW  G +P+  T KG+QR  GF VFNDTFRNA+RGS    EE F++DGG +  VK GI+GS+D FA SPLE INYVECHDNRTLWDHL + IR R D  I ++  DV R+ KLAAV++LTSQGIPF+ +GQEM R+KFG ENSY+S D+IN +RW  K++ +  V Y RGL+ LRRA P+IF M +A ++   I FYE  GL VP++C+ YRI      +    Q E +        +E++P  W  V +LFN TP+  +F LP +  D +   IV+   A      +L    IG   V GRSA VLR  + SE   +  E RL +I++ + S
Sbjct: 1003 DEDFPVEEVMVHLKGFSPSRGAWRRYENVDDYYYSGALGWHYTKSATTFRVFAPTADLVVLCLYREPVGTVGRQAFPMRRIPQGVWKIVVHGDLQGLYYTYLAEGVNKRLFPGVEVIDPYSRCNTAHNGRGLIFGEDHTEVADRPCIPPEHTIIYEVHLRDFTIDECSGVPEKLRGKYLGLAQQGTQLPEHWVRSRIVSSAGRAALFLRDRATPDAFEEDVCPSTVEEDVYTAPVLTEVAAQVDWTDRSTCLDHVRFLGVNALQIMPVQDFDNDESNPLEYRWGYMPVHFFSPDGWYASCTSDASRVRELKQLIDTLHRAGLKVILDMVLNHTAEDPNEFNLEARFSFNGLAPRYYYRFW---PDGSWGVSEA------------CYANGSGCGNEFRSESPMGRKFILDMLWYWATEYKVDGFRFDLMGLMDRETLRIAAAKLHALDPNILVYGEPWSAGETPIIITGKGAQRHLGFGVFNDTFRNALRGSNQGIEENFLLDGGCIEAVKQGILGSIDDFAASPLEVINYVECHDNRTLWDHLWYVIRERADANITYTAADVLRVCKLAAVILLTSQGIPFLHSGQEMARSKFGVENSYQSGDDINRLRWERKIDFYGLVVYVRGLVQLRRARPDIFSMSDAQQVRRCIAFYEALGLPVPERCLAYRI------IADAGQSETNA-------IEDDP--WRCVALLFNPTPVAQVFPLPPEAKDELMTVIVNDLEAPRDAGASLGEYHIGWASVPGRSAMVLRLCTESERERALIETRLNAISEPYSS 1761          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A1Y1RKU5_9BACT (Pullulanase n=1 Tax=Candidatus Cloacimonetes bacterium 4572_55 TaxID=1971726 RepID=A0A1Y1RKU5_9BACT)

HSP 1 Score: 621 bits (1601), Expect = 4.920e-200
Identity = 369/957 (38.56%), Postives = 504/957 (52.66%), Query Frame = 0

Query:  484 RVRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPVSVTKDW-TDFIVDRASYGSAKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEHSVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVH--FDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDT---EETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYE-DCGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGSVEVSGRSAAVL 1433
            RV Y R+   +Y GW+LWTWD       R A+ P+     +   F++D+  YGS + I LLPR+G   WLE+D  +R+W  +L          G E+  +  D   + E P               D    +  A++D+   V V  P+ + +     +  + +         G P+ +     +++  ++   +L              L+  TS     FD    +    V V  F   GL +    +   Y  +  LG  Y   Q +FR FAP A +V++ IY +     G+   P+     G W+  + ++LK  YY    +G      P  E+IDPYSRCNTAH GRG+I   + T + DRP     + IIYELH+RD +I+  SGI   G +LG+ E+G                            KD     +  +GLDH+ +MGV  +QIMPIQDFDNNE  E  Y WGYMPVHFNSPDGWYA+    A RV+EFKKLV A H  G+RVIMDVVYNHTAE     N   ++SFNG+AP YYYR                       R    + NGSGCGNEFRSE+PM RKFI+DS+ +W +EY VDGFRFDL+GLID+ET+ Q  E L  IDP+I+VYGEPW  G +P+  T KG QR  GF VFND FR+A++GSPFD     ETF++ G R+ E+K GI GS+ +FA  PLES+NY+ECHDNRTLWD L++  + +TD       D+ +M KL AV++ TSQG+PFIQ GQEM RTK   ENSY +PD +N I W  K+     V+Y++GLI LR+AH ++F   +   I + + F + D G+ VPD+CI Y+                         LE+E   W  V++L N  P+ V+F +P       W  +V  + +G   +         V+ RSAAVL
Sbjct:   28 RVHYHRYD-GNYEGWNLWTWDKVSNDYSR-AIQPLKKRDHYGLTFVLDKTHYGSGEMIGLLPRKG--EWLEKDDPDRIWTPNL----------GNEAWILQNDPDLFVEPP---------------DTYPQISGAFIDAPRRVTVCLPISIRFDPEQAEHVRIIDDQ------GQPIPI----QSVAPGEIGNAAL--------------LLINTSDSFRAFDLAMPIHKYRVEVLGFKPKGLSFRMLMDRPEYVSDVELGAIYKPDQTLFRLFAPTATRVTLTIYDQPDRDIGQAY-PLESQASGVWEIDIKQNLKNKYYTYKVDGNSPLFHPDQELIDPYSRCNTAHNGRGMIID-DGTRIADRPDFPPEDAIIYELHVRDFSIDTDSGINQKGLYLGMTEQG-------------------------ATYKD---QGEVKTGLDHLVEMGVNTVQIMPIQDFDNNESSE-EYNWGYMPVHFNSPDGWYATEQRNAKRVEEFKKLVDALHTRGIRVIMDVVYNHTAET----NPYVQYSFNGIAPGYYYRM----------------------RDSGSFWNGSGCGNEFRSEAPMVRKFILDSVAYWVNEYKVDGFRFDLMGLIDLETMTQITETLHEIDPNILVYGEPWASGETPISITSKGKQRGLGFGVFNDNFRDALKGSPFDRFDISETFLIHGSRISEIKKGIEGSIHSFAQRPLESVNYMECHDNRTLWDQLHYLTQGQTD---IDARDIVQMVKLGAVIIFTSQGVPFIQMGQEMLRTKHDVENSYNAPDSVNQIEWERKIRYRDVVEYHKGLIQLRKAH-QMFRQTDPQAIEKNLKFLDNDLGMTVPDKCIAYQ-------------------------LEDERDEWERVLVLLNPNPISVIFPIP----PGTWTSVVDENQSGVEPIQIGIEREKRVAPRSAAVL 841          

BLAST of Gchil5632.t1 vs. uniprot
Match: A0A3M2EUP4_9BACT (Pullulanase n=1 Tax=Candidatus Hydrogenedentes bacterium TaxID=2030809 RepID=A0A3M2EUP4_9BACT)

HSP 1 Score: 613 bits (1581), Expect = 2.400e-195
Identity = 381/953 (39.98%), Postives = 498/953 (52.26%), Query Frame = 0

Query:  485 VRYRRFSPSDYNGWDLWTWDPTDPASHRVALAPVSVTKDWTDFIVDRASYGS-AKNICLLPRRGGKAWLERDGVERVWNASLLEDAHGMEAKGTESDFISTDTHNYDEFPTFTITQDTTFVLRSLDEVKCMLDAYVDSEH--SVVVSTPVPLGWICPTKKAAKQLGQNVCIKLCGHPVQLICKEGNLSYNQVTGKSLRHKKSEQLSTTKTRLMFETSVVHFDEDFLVENVIVCVHPFHAVGLHWERHQNEDRYFYEGSLGWSYSARQCVFRCFAPAADKVSVVIYAKATGAEGRVVVPMRRIPQGCWKAIVSKDLKGAYYKLLAEGKCKRLFPGLEVIDPYSRCNTAHAGRGLIYGTETTPVHDRPSLRAAEIIIYELHIRDMTIENSSGIRNGGKFLGLAERGTKMVPTTTKHPQRKPLTPWEQEIMPGVQKDFFVLEKFSSGLDHIAQMGVTAIQIMPIQDFDNNEDDEFAYRWGYMPVHFNSPDGWYASSTHTAARVKEFKKLVSAAHKAGLRVIMDVVYNHTAEDSNELNLEARFSFNGLAPRYYYRTCANTPVAHNGESTCGRRAPHEPRCGECYSNGSGCGNEFRSESPMGRKFIVDSLKFWASEYGVDGFRFDLLGLIDVETIQQAVEELKAIDPDIVVYGEPWIGGLSPMKTTDKGSQRSKGFAVFNDTFRNAIRGSPFDTEETFIMDGGRLMEVKGGIIGSVDTFADSPLESINYVECHDNRTLWDHLNHYIRSRTDTIKFSDEDVRRMHKLAAVLVLTSQGIPFIQAGQEMCRTKFGDENSYESPDEINMIRWSTKVEEWSTVQYYRGLILLRRAHPEIFCMENADEIHEKIVFYED-CGLHVPDQCIGYRIKGNPEKLLRRLQEERSEDVPGEQDLEEEPQRWSEVVILFNATPLKVLFELPGKEADVMWMQIVSASAAGTRTLSGPFIGS-VEVSGRSAAV 1432
            V Y R    DY GW LWTW+P    S  +   P         F +D+  YGS    I LLP+ G   W  +D  +R W  SL          G     +S D + Y   P  T  +    +L  ++EV+ +L   +D  +     VS  +P     P  +A    GQ + ++       L+     +    V G                      S   +    L+   +V                  D Y Y+G LG  YS  + +FR F+P +  VSVVIY   TG   R VVPM+RI QG W+AIV  +L   YYK     + K      EVIDPYS CNTAH GRG+I   + TPV   P    A+ +IYE+HIRD TI+  SGI++ GK+LGL E GT++                  E  P +          S+GLDHI ++GV  +QIMP QDFDN+E  E AY WGYMP HFNSPDGWYA+    A+RV+E KK++ A H+ G++V+MDVVYNHTAE     N E R SFNGLAP YYYR      V  +G                 Y NGSG GNEFR+E+PM RKFIVDS+K+W  +Y VDGFRFDL+GLID+ET++  V EL+ I P+I++YGEPW GG +P+K T KG QR KGFAVFND FR+A++G  F+TE  FI DG  + +VK GI+GS+D F D+P E+INYV  HDN TLWD +    R+ T   K   +D  +M KLAAV +LT+QGIPF+  GQ++ RTK G+ENSY  PD INMI W  K +     +YYRGLI LRRAHP +F    AD +   + F +D   L VP + IG+ +                          E    W  V++L NA+     FE+P       W+ + +   AG   L  P   S V +  RS+A+
Sbjct:   79 VHYHR-PEGDYTGWTLWTWNPASGTSREIT--PAGTDGYGLVFRLDKNDYGSNVDRIGLLPKYGD--WKAKDDPDRYWTPSL----------GRNVFILSADKNLYSS-PPDTRPRILRALLDKVNEVRVVLSKSIDRSYVRKGTVSVRLPDRSAVPVVEAVPAPGQGLKVRTI-----LVRLGQEIDPESVAG-------------------IVVSFGAYRPAPLIPGAVV------------------DSYRYDGELGALYSPEKTIFRTFSPTSAGVSVVIYEGPTGPP-RDVVPMKRIGQGIWEAIVEGNLLDKYYKY----RVKYPDAEYEVIDPYSHCNTAHNGRGMIID-DNTPVSPAPRFGIADAVIYEIHIRDFTIDPKSGIQHRGKYLGLTEEGTRL------------------EGNPEI----------STGLDHIKELGVNTVQIMPFQDFDNDEASE-AYNWGYMPFHFNSPDGWYATKRDDASRVREAKKMIDAFHRNGIKVVMDVVYNHTAEG----NPEVRMSFNGLAPNYYYR------VKDDGS----------------YWNGSGTGNEFRTEAPMARKFIVDSMKYWVRKYDVDGFRFDLMGLIDLETLETIVRELRKIKPEIMIYGEPWAGGSTPVKVTSKGDQRGKGFAVFNDHFRDALKGGTFNTEPGFIQDGRDVEKVKKGILGSIDDFTDAPTETINYVAAHDNHTLWDRILLTTRNWTQVTK---KDREQMDKLAAVAILTAQGIPFLHGGQDLLRTKDGEENSYNKPDRINMIHWEWKKKHREVFEYYRGLIALRRAHP-LFRKTTADAVRRGVFFLDDDLELRVPPRAIGFVVNRG-----------------------EAQDEWENVLVLMNASATSARFEIPASH----WIPVANGEKAGVTPLGPPVTRSEVILPPRSSAI 881