Gchil8251.t1 (mRNA) Gracilaria chilensis NLEC103_M9 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	Gchil8251.t1
Unique Name	Gchil8251.t1
Type	mRNA
Organism	Gracilaria chilensis NLEC103_M9 male (Gracilaria chilensis NLEC103_M9 male)
Sequence length	728

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
tig00004384_pilon	contig	tig00004384_pilon:877706..879889 -

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Gracilaria chilensis NLEC103_M9 male OGS1.0	2022-05-09

Properties


Property Name	Value
Seed ortholog	130081.XP_005707443.1
Preferred name	TAF5
PFAMs	LisH,TFIID_NTD2,WD40
Max annot lvl	2759\|Eukaryota
KEGG ko	ko:K03130
KEGG Pathway	ko03022,ko05168,map03022,map05168
GOs	GO:0000123,GO:0000124,GO:0000428,GO:0000988,GO:0000990,GO:0000991,GO:0003674,GO:0003682,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005667,GO:0005669,GO:0006139,GO:0006325,GO:0006338,GO:0006351,GO:0006352,GO:0006355,GO:0006357,GO:0006366,GO:0006367,GO:0006464,GO:0006473,GO:0006475,GO:0006725,GO:0006807,GO:0006996,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009889,GO:0009987,GO:0010467,GO:0010468,GO:0010556,GO:0016043,GO:0016070,GO:0016569,GO:0016570,GO:0016573,GO:0016591,GO:0018130,GO:0018193,GO:0018205,GO:0018393,GO:0018394,GO:0019219,GO:0019222,GO:0019438,GO:0019538,GO:0022607,GO:0030880,GO:0031248,GO:0031323,GO:0031326,GO:0031974,GO:0031981,GO:0032182,GO:0032774,GO:0032947,GO:0032991,GO:0034622,GO:0034641,GO:0034645,GO:0034654,GO:0036211,GO:0043130,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043543,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044451,GO:0044464,GO:0044798,GO:0046483,GO:0046695,GO:0050789,GO:0050794,GO:0051123,GO:0051171,GO:0051252,GO:0051276,GO:0055029,GO:0060255,GO:0061695,GO:0065003,GO:0065004,GO:0065007,GO:0070013,GO:0070461,GO:0070897,GO:0071704,GO:0071824,GO:0071840,GO:0080090,GO:0090304,GO:0090575,GO:0097659,GO:0140110,GO:1901360,GO:1901362,GO:1901564,GO:1901576,GO:1902493,GO:1902494,GO:1903506,GO:1905368,GO:1990234,GO:2000112,GO:2001141
Evalue	5.23e-48
EggNOG OGs	COG2319@1\|root,KOG0263@2759\|Eukaryota
Description	transcription initiation from RNA polymerase II promoter
COG category	Q
BRITE	ko00000,ko00001,ko03021,ko03036

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
Gchil8251	Gchil8251	Gracilaria chilensis NLEC103_M9 male	gene	tig00004384_pilon 877706..879889 -

The following stop_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gchil8251.t1.stop1	Gchil8251.t1.stop1	Gracilaria chilensis NLEC103_M9 male	stop_codon	tig00004384_pilon 877706..877708 -

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gchil8251.t1.CDS1	Gchil8251.t1.CDS1	Gracilaria chilensis NLEC103_M9 male	CDS	tig00004384_pilon 877706..879889 -

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gchil8251.t1.exon1	Gchil8251.t1.exon1	Gracilaria chilensis NLEC103_M9 male	exon	tig00004384_pilon 877706..879889 -

The following start_codon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gchil8251.t1.start1	Gchil8251.t1.start1	Gracilaria chilensis NLEC103_M9 male	start_codon	tig00004384_pilon 879887..879889 -

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
Gchil8251.t1	Gchil8251.t1	Gracilaria chilensis NLEC103_M9 male	polypeptide	tig00004384_pilon 877706..879889 -

Sequences

The following sequences are available for this feature:

mRNA sequence
mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at tig00004384_pilon:877706..879889-
coding sequence from alignment at tig00004384_pilon:877706..879889-

mRNA sequence

>Gchil8251.t1 ID=Gchil8251.t1|Name=Gchil8251.t1|organism=Gracilaria chilensis NLEC103_M9 male|type=mRNA|length=728bp
MRVNAAEKVSSDYLRKHGYVSETSVAGLGTAQRVSPQQASPPDLGIEHAE
VDDEHRNTLMMLQRPELVAAQDTRRFEDSYCELRDWVDGSLDIYKAEFHA
LLYPLLVHCFLQMIKLKATAEARAFLSRCSAEFREGTAAQRRELTSLSGI
ATPQHLEENEVSNLFLKNRYEVHLSQYAFELLMAFLGDDPRRVVLVRILN
MHLAVRMDEGIEASRTGGFIVKAKEDRAVESGFVPSKDRDALLRTDVLWG
RLRPEHYVIPDEDDAAVKNQKSKPKPTGEKAKQSNETKTKADNPKKDIAE
EEEEPAVREDGTLTKSRIPLKKYRVGSKGIEAPLDRKNRAKLFDAVTMQS
SCDELAILCYTFTNTLEDGLNCSASSEDGSLVVAGFGDSSLRIWDAKSSS
TESGRGGLTGDGTDRLIGHSGPVYAVDWTKCGRFVLSGSEDGSLRLWSAD
YQRDVCSYRGHNYPVWSAAFSPLDHYFVSGGHDRTARVWSTDCLIPLRVF
AGHLADVDCVRWHPNCNYVATGSSDRTARLWDVRDGKCVRVFGTHRGTIH
SLAFSPDGRTLACGGDADTIDIWDVASGKRMRRLSGHSSTVWSLDYSRKG
AILASGGADCKVCVWRATDWSSIIHSTEEDMWQNGDVANEQESNGVDADG
DVRMEGEANGKGDDEESRDKDVDDAEEDRKEAVKKRAEQQRARGSSLLES
FETKDTPIHSVKFTRKNVLVAIGSFRG*

spliced messenger RNA

>Gchil8251.t1 ID=Gchil8251.t1|Name=Gchil8251.t1|organism=Gracilaria chilensis NLEC103_M9 male|type=mRNA|length=2184bp|location=Sequence derived from alignment at tig00004384_pilon:877706..879889- (Gracilaria chilensis NLEC103_M9 male)|Notes=Excludes all bases but those of type(s): exon.  
ATGCGTGTTAATGCAGCTGAGAAGGTTTCTTCTGACTACCTCCGCAAACA
TGGATATGTATCTGAGACTTCTGTGGCAGGCCTTGGAACAGCGCAACGTG
TCTCTCCTCAACAAGCTTCTCCACCCGACCTTGGAATTGAACACGCTGAA
GTTGATGACGAACATCGTAACACCCTCATGATGCTCCAACGCCCTGAGCT
TGTGGCCGCTCAAGATACCAGGCGCTTTGAAGATAGCTACTGCGAGCTTC
GCGATTGGGTGGACGGAAGTTTGGATATCTACAAGGCAGAGTTTCACGCG
TTGCTCTATCCGCTGCTTGTGCACTGCTTTCTTCAGATGATAAAGCTAAA
AGCTACGGCAGAAGCTAGAGCATTTCTGTCTCGTTGCTCAGCAGAGTTTC
GAGAGGGCACTGCCGCCCAGCGTCGGGAACTTACATCGTTATCTGGAATT
GCGACACCACAACATCTGGAGGAGAACGAAGTCTCAAACCTCTTTCTAAA
AAATCGCTATGAAGTGCATTTGTCACAGTATGCTTTCGAACTTTTGATGG
CGTTTCTCGGCGATGACCCAAGGCGGGTAGTTCTGGTACGCATACTCAAT
ATGCACCTTGCTGTGCGAATGGACGAAGGGATCGAGGCATCACGAACAGG
GGGGTTTATTGTAAAGGCAAAAGAAGACCGAGCTGTCGAGTCAGGCTTCG
TACCTTCCAAAGATCGAGATGCTTTATTGCGAACTGATGTGTTGTGGGGT
CGATTGCGCCCAGAGCACTATGTTATACCGGACGAAGATGATGCCGCCGT
GAAGAACCAAAAAAGCAAACCGAAGCCGACTGGAGAAAAAGCCAAGCAGT
CGAACGAGACAAAAACAAAGGCGGATAATCCCAAGAAGGATATTGCTGAG
GAAGAGGAAGAACCCGCTGTTCGTGAGGATGGAACACTGACGAAGTCAAG
AATCCCGCTAAAGAAATATAGAGTGGGTTCAAAAGGCATCGAAGCGCCTC
TTGATCGAAAGAACCGTGCAAAGCTTTTCGATGCAGTTACAATGCAAAGC
AGTTGCGATGAACTTGCTATTCTGTGTTACACCTTTACCAATACGCTTGA
GGATGGTCTTAATTGTTCAGCCTCGTCCGAGGATGGGTCTTTAGTGGTTG
CTGGATTCGGCGACTCTTCGCTGCGCATATGGGATGCCAAGAGCTCAAGT
ACGGAGAGTGGACGAGGTGGATTAACGGGGGATGGGACAGATCGACTAAT
TGGACACTCAGGACCTGTGTACGCAGTTGACTGGACAAAATGCGGCCGTT
TTGTTTTAAGTGGGTCTGAAGATGGAAGTTTGCGTTTATGGTCCGCAGAC
TATCAAAGGGATGTCTGCTCGTACAGGGGTCACAATTATCCTGTATGGAG
TGCAGCCTTTTCTCCTCTAGACCATTATTTTGTGAGTGGTGGGCACGATC
GCACAGCGCGGGTGTGGAGCACTGACTGCCTGATACCGCTCCGAGTTTTT
GCTGGTCACCTCGCTGATGTGGATTGTGTGCGATGGCATCCCAATTGCAA
CTACGTGGCGACAGGATCAAGTGACCGCACGGCGCGCTTGTGGGATGTGC
GAGATGGGAAATGTGTGCGCGTTTTCGGGACACACCGCGGTACTATTCAT
TCCTTGGCGTTTTCTCCTGACGGGCGCACATTGGCGTGTGGTGGGGATGC
TGACACCATAGACATCTGGGATGTTGCGAGTGGGAAACGCATGCGGCGTT
TGAGTGGGCACTCGTCTACAGTGTGGTCCCTTGACTATTCGCGAAAGGGA
GCGATTCTTGCATCAGGAGGGGCAGATTGCAAGGTATGCGTATGGCGAGC
GACAGACTGGTCAAGTATCATTCATTCTACAGAGGAGGACATGTGGCAGA
ATGGAGATGTGGCGAACGAGCAGGAGAGCAACGGCGTGGATGCGGACGGC
GACGTGCGAATGGAGGGGGAGGCAAATGGGAAAGGAGATGATGAAGAAAG
CAGAGACAAGGATGTAGACGACGCGGAAGAGGATAGGAAGGAGGCGGTGA
AGAAACGCGCGGAGCAGCAGAGAGCGCGCGGATCGTCTCTGCTGGAGTCA
TTCGAAACGAAGGACACGCCTATTCACTCGGTGAAGTTTACGCGAAAGAA
TGTGCTGGTGGCAATTGGCAGCTTCCGGGGCTAG

protein sequence of Gchil8251.t1

>Gchil8251.t1 ID=Gchil8251.t1|Name=Gchil8251.t1|organism=Gracilaria chilensis NLEC103_M9 male|type=polypeptide|length=728bp
MRVNAAEKVSSDYLRKHGYVSETSVAGLGTAQRVSPQQASPPDLGIEHAE
VDDEHRNTLMMLQRPELVAAQDTRRFEDSYCELRDWVDGSLDIYKAEFHA
LLYPLLVHCFLQMIKLKATAEARAFLSRCSAEFREGTAAQRRELTSLSGI
ATPQHLEENEVSNLFLKNRYEVHLSQYAFELLMAFLGDDPRRVVLVRILN
MHLAVRMDEGIEASRTGGFIVKAKEDRAVESGFVPSKDRDALLRTDVLWG
RLRPEHYVIPDEDDAAVKNQKSKPKPTGEKAKQSNETKTKADNPKKDIAE
EEEEPAVREDGTLTKSRIPLKKYRVGSKGIEAPLDRKNRAKLFDAVTMQS
SCDELAILCYTFTNTLEDGLNCSASSEDGSLVVAGFGDSSLRIWDAKSSS
TESGRGGLTGDGTDRLIGHSGPVYAVDWTKCGRFVLSGSEDGSLRLWSAD
YQRDVCSYRGHNYPVWSAAFSPLDHYFVSGGHDRTARVWSTDCLIPLRVF
AGHLADVDCVRWHPNCNYVATGSSDRTARLWDVRDGKCVRVFGTHRGTIH
SLAFSPDGRTLACGGDADTIDIWDVASGKRMRRLSGHSSTVWSLDYSRKG
AILASGGADCKVCVWRATDWSSIIHSTEEDMWQNGDVANEQESNGVDADG
DVRMEGEANGKGDDEESRDKDVDDAEEDRKEAVKKRAEQQRARGSSLLES
FETKDTPIHSVKFTRKNVLVAIGSFRG*

mRNA from alignment at tig00004384_pilon:877706..879889-

Legend: polypeptideCDSexonstart_codonstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

>Gchil8251.t1 ID=Gchil8251.t1|Name=Gchil8251.t1|organism=Gracilaria chilensis NLEC103_M9 male|type=mRNA|length=2184bp|location=Sequence derived from alignment at tig00004384_pilon:877706..879889- (Gracilaria chilensis NLEC103_M9 male)
ATGCGTGTTAATGCAGCTGAGAAGGTTTCTTCTGACTACCTCCGCAAACA
TGGATATGTATCTGAGACTTCTGTGGCAGGCCTTGGAACAGCGCAACGTG
TCTCTCCTCAACAAGCTTCTCCACCCGACCTTGGAATTGAACACGCTGAA
GTTGATGACGAACATCGTAACACCCTCATGATGCTCCAACGCCCTGAGCT
TGTGGCCGCTCAAGATACCAGGCGCTTTGAAGATAGCTACTGCGAGCTTC
GCGATTGGGTGGACGGAAGTTTGGATATCTACAAGGCAGAGTTTCACGCG
TTGCTCTATCCGCTGCTTGTGCACTGCTTTCTTCAGATGATAAAGCTAAA
AGCTACGGCAGAAGCTAGAGCATTTCTGTCTCGTTGCTCAGCAGAGTTTC
GAGAGGGCACTGCCGCCCAGCGTCGGGAACTTACATCGTTATCTGGAATT
GCGACACCACAACATCTGGAGGAGAACGAAGTCTCAAACCTCTTTCTAAA
AAATCGCTATGAAGTGCATTTGTCACAGTATGCTTTCGAACTTTTGATGG
CGTTTCTCGGCGATGACCCAAGGCGGGTAGTTCTGGTACGCATACTCAAT
ATGCACCTTGCTGTGCGAATGGACGAAGGGATCGAGGCATCACGAACAGG
GGGGTTTATTGTAAAGGCAAAAGAAGACCGAGCTGTCGAGTCAGGCTTCG
TACCTTCCAAAGATCGAGATGCTTTATTGCGAACTGATGTGTTGTGGGGT
CGATTGCGCCCAGAGCACTATGTTATACCGGACGAAGATGATGCCGCCGT
GAAGAACCAAAAAAGCAAACCGAAGCCGACTGGAGAAAAAGCCAAGCAGT
CGAACGAGACAAAAACAAAGGCGGATAATCCCAAGAAGGATATTGCTGAG
GAAGAGGAAGAACCCGCTGTTCGTGAGGATGGAACACTGACGAAGTCAAG
AATCCCGCTAAAGAAATATAGAGTGGGTTCAAAAGGCATCGAAGCGCCTC
TTGATCGAAAGAACCGTGCAAAGCTTTTCGATGCAGTTACAATGCAAAGC
AGTTGCGATGAACTTGCTATTCTGTGTTACACCTTTACCAATACGCTTGA
GGATGGTCTTAATTGTTCAGCCTCGTCCGAGGATGGGTCTTTAGTGGTTG
CTGGATTCGGCGACTCTTCGCTGCGCATATGGGATGCCAAGAGCTCAAGT
ACGGAGAGTGGACGAGGTGGATTAACGGGGGATGGGACAGATCGACTAAT
TGGACACTCAGGACCTGTGTACGCAGTTGACTGGACAAAATGCGGCCGTT
TTGTTTTAAGTGGGTCTGAAGATGGAAGTTTGCGTTTATGGTCCGCAGAC
TATCAAAGGGATGTCTGCTCGTACAGGGGTCACAATTATCCTGTATGGAG
TGCAGCCTTTTCTCCTCTAGACCATTATTTTGTGAGTGGTGGGCACGATC
GCACAGCGCGGGTGTGGAGCACTGACTGCCTGATACCGCTCCGAGTTTTT
GCTGGTCACCTCGCTGATGTGGATTGTGTGCGATGGCATCCCAATTGCAA
CTACGTGGCGACAGGATCAAGTGACCGCACGGCGCGCTTGTGGGATGTGC
GAGATGGGAAATGTGTGCGCGTTTTCGGGACACACCGCGGTACTATTCAT
TCCTTGGCGTTTTCTCCTGACGGGCGCACATTGGCGTGTGGTGGGGATGC
TGACACCATAGACATCTGGGATGTTGCGAGTGGGAAACGCATGCGGCGTT
TGAGTGGGCACTCGTCTACAGTGTGGTCCCTTGACTATTCGCGAAAGGGA
GCGATTCTTGCATCAGGAGGGGCAGATTGCAAGGTATGCGTATGGCGAGC
GACAGACTGGTCAAGTATCATTCATTCTACAGAGGAGGACATGTGGCAGA
ATGGAGATGTGGCGAACGAGCAGGAGAGCAACGGCGTGGATGCGGACGGC
GACGTGCGAATGGAGGGGGAGGCAAATGGGAAAGGAGATGATGAAGAAAG
CAGAGACAAGGATGTAGACGACGCGGAAGAGGATAGGAAGGAGGCGGTGA
AGAAACGCGCGGAGCAGCAGAGAGCGCGCGGATCGTCTCTGCTGGAGTCA
TTCGAAACGAAGGACACGCCTATTCACTCGGTGAAGTTTACGCGAAAGAA
TGTGCTGGTGGCAATTGGCAGCTTCCGGGGCTAG

Coding sequence (CDS) from alignment at tig00004384_pilon:877706..879889-

>Gchil8251.t1 ID=Gchil8251.t1|Name=Gchil8251.t1|organism=Gracilaria chilensis NLEC103_M9 male|type=CDS|length=2184bp|location=Sequence derived from alignment at tig00004384_pilon:877706..879889- (Gracilaria chilensis NLEC103_M9 male)
ATGCGTGTTAATGCAGCTGAGAAGGTTTCTTCTGACTACCTCCGCAAACA
TGGATATGTATCTGAGACTTCTGTGGCAGGCCTTGGAACAGCGCAACGTG
TCTCTCCTCAACAAGCTTCTCCACCCGACCTTGGAATTGAACACGCTGAA
GTTGATGACGAACATCGTAACACCCTCATGATGCTCCAACGCCCTGAGCT
TGTGGCCGCTCAAGATACCAGGCGCTTTGAAGATAGCTACTGCGAGCTTC
GCGATTGGGTGGACGGAAGTTTGGATATCTACAAGGCAGAGTTTCACGCG
TTGCTCTATCCGCTGCTTGTGCACTGCTTTCTTCAGATGATAAAGCTAAA
AGCTACGGCAGAAGCTAGAGCATTTCTGTCTCGTTGCTCAGCAGAGTTTC
GAGAGGGCACTGCCGCCCAGCGTCGGGAACTTACATCGTTATCTGGAATT
GCGACACCACAACATCTGGAGGAGAACGAAGTCTCAAACCTCTTTCTAAA
AAATCGCTATGAAGTGCATTTGTCACAGTATGCTTTCGAACTTTTGATGG
CGTTTCTCGGCGATGACCCAAGGCGGGTAGTTCTGGTACGCATACTCAAT
ATGCACCTTGCTGTGCGAATGGACGAAGGGATCGAGGCATCACGAACAGG
GGGGTTTATTGTAAAGGCAAAAGAAGACCGAGCTGTCGAGTCAGGCTTCG
TACCTTCCAAAGATCGAGATGCTTTATTGCGAACTGATGTGTTGTGGGGT
CGATTGCGCCCAGAGCACTATGTTATACCGGACGAAGATGATGCCGCCGT
GAAGAACCAAAAAAGCAAACCGAAGCCGACTGGAGAAAAAGCCAAGCAGT
CGAACGAGACAAAAACAAAGGCGGATAATCCCAAGAAGGATATTGCTGAG
GAAGAGGAAGAACCCGCTGTTCGTGAGGATGGAACACTGACGAAGTCAAG
AATCCCGCTAAAGAAATATAGAGTGGGTTCAAAAGGCATCGAAGCGCCTC
TTGATCGAAAGAACCGTGCAAAGCTTTTCGATGCAGTTACAATGCAAAGC
AGTTGCGATGAACTTGCTATTCTGTGTTACACCTTTACCAATACGCTTGA
GGATGGTCTTAATTGTTCAGCCTCGTCCGAGGATGGGTCTTTAGTGGTTG
CTGGATTCGGCGACTCTTCGCTGCGCATATGGGATGCCAAGAGCTCAAGT
ACGGAGAGTGGACGAGGTGGATTAACGGGGGATGGGACAGATCGACTAAT
TGGACACTCAGGACCTGTGTACGCAGTTGACTGGACAAAATGCGGCCGTT
TTGTTTTAAGTGGGTCTGAAGATGGAAGTTTGCGTTTATGGTCCGCAGAC
TATCAAAGGGATGTCTGCTCGTACAGGGGTCACAATTATCCTGTATGGAG
TGCAGCCTTTTCTCCTCTAGACCATTATTTTGTGAGTGGTGGGCACGATC
GCACAGCGCGGGTGTGGAGCACTGACTGCCTGATACCGCTCCGAGTTTTT
GCTGGTCACCTCGCTGATGTGGATTGTGTGCGATGGCATCCCAATTGCAA
CTACGTGGCGACAGGATCAAGTGACCGCACGGCGCGCTTGTGGGATGTGC
GAGATGGGAAATGTGTGCGCGTTTTCGGGACACACCGCGGTACTATTCAT
TCCTTGGCGTTTTCTCCTGACGGGCGCACATTGGCGTGTGGTGGGGATGC
TGACACCATAGACATCTGGGATGTTGCGAGTGGGAAACGCATGCGGCGTT
TGAGTGGGCACTCGTCTACAGTGTGGTCCCTTGACTATTCGCGAAAGGGA
GCGATTCTTGCATCAGGAGGGGCAGATTGCAAGGTATGCGTATGGCGAGC
GACAGACTGGTCAAGTATCATTCATTCTACAGAGGAGGACATGTGGCAGA
ATGGAGATGTGGCGAACGAGCAGGAGAGCAACGGCGTGGATGCGGACGGC
GACGTGCGAATGGAGGGGGAGGCAAATGGGAAAGGAGATGATGAAGAAAG
CAGAGACAAGGATGTAGACGACGCGGAAGAGGATAGGAAGGAGGCGGTGA
AGAAACGCGCGGAGCAGCAGAGAGCGCGCGGATCGTCTCTGCTGGAGTCA
TTCGAAACGAAGGACACGCCTATTCACTCGGTGAAGTTTACGCGAAAGAA
TGTGCTGGTGGCAATTGGCAGCTTCCGGGGCTAG