Gcaud9918.t1 (polypeptide) Gracilaria caudata M_176_S67 male

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A2V3J2P7_9FLOR (Mitotic spindle assembly checkpoint protein MAD1 n=1 Tax=Gracilariopsis chorda TaxID=448386 RepID=A0A2V3J2P7_9FLOR)

HSP 1 Score: 771 bits (1991), Expect = 5.130e-270
Identity = 436/743 (58.68%), Postives = 569/743 (76.58%), Query Frame = 0

Query:    1 MDREDETPAAPSDNAWLRVSNTIRSLQSEIRVLQDPAVRSALQTPPSAPRTPAELNAKIVSLRTELSHREQKLREEKEATLAARQRAARAEGELQALHRQNEETSTLHERAVSDATSRIEELETRLAEHEASVRMHRVRQRDERERGRISDTSFASEREMLTTQAKVAEAKAKRLERELVAEKDRNVAEQKELQDKYDWAYGYAKRLQKEIDHLSNTRSSFGEFRDANTSDLRKKNVSLESEVFRMRSQLRDESVNRTRMGARLAEMGAENDRLRELCRNAQQNEEQSKYMNEEITLLRIQASEEEQAVRNASALQSEKEELARLISALSPTGDAQEGIQILRSLANGAGGNKSSDIAQRIAAQKYEVQIAEIEEQHSIKLLRLQDELDDCRRIKSEMRNENIRLKEDKEAASAQQKRTELGRRILQREIDHLRAALREIEEVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIEELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVEKKHKEREHLDKDVDMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGAKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTTLMNI 743
            +D ++E+    +D  WLRV +TIRSLQS++RVLQDPAVR+ALQTP + PR+PAELNA IVSLRTELS RE+KLREEK+ TL ARQRAARAEGE+ A  R+  ETSTLHE AV  A  RI +LE +LA++E SV M RVR+RD+R+R R+ D SFA+EREM  TQ ++A+AK +RLEREL +  +R   + K+LQD+Y+ A   A RL+KE + +S +R SF E RD N + L++ N SLE+EV+++R+QLRD++  R R   +L EM +  DRLRE CR A+  EE S++++EEITLLRIQASEEE+A RNAS LQSEKEEL RLI ALSPTGDAQEGI+ LRSLA     + S D+AQR+A Q+YEV++A IE+Q+SIK +R+QD+LDDC+R   EM  EN ++K D  AA  +++R E GRRILQRE++HLRAALREIEEVEI GT   DDGR RRR+KA ED+ +E +SA+ ++EKEL++K+KQI +LRS+  G F    SSD +  ++  +E +L SE  RR + EK+++ L++ L   + ++E+  A +A L PD S  D+DPT+VK +HL DNPL +A+ D+AM   ++K   R+  D DVDM D  NSE+  L+  +++L++EN  L+K+SKVGLRTKEIAMKKI EVR AVYNL GWSM++AGAKYT+ SIYAESPNE+LEFG NE+GTFALM+NHYTNQL EEIEQYV+KM+S+P LLA ITMEN+EKTTLMN+
Sbjct:    3 VDDDNESHHPSTDEVWLRVDDTIRSLQSDVRVLQDPAVRTALQTPTATPRSPAELNAAIVSLRTELSLREKKLREEKQTTLVARQRAARAEGEILAFRRERAETSTLHENAVQQAAERITQLEKQLADYEHSVNMQRVRERDQRQRDRMHDNSFAAEREMYNTQIQMADAKIRRLERELTSLGERFQVQNKQLQDRYNLALTRADRLEKEKEQISTSRISFSEVRDRNITSLQENNRSLEAEVYKLRTQLRDQTQLRARTVEKLVEMESAVDRLREQCREAESKEENSRFLSEEITLLRIQASEEERAQRNASTLQSEKEELTRLICALSPTGDAQEGIRTLRSLALTQADSGSVDMAQRVAMQQYEVRLAAIEQQNSIKAMRIQDQLDDCKRRLVEMDAENKQVKSDWAAALDEKRRYERGRRILQREVEHLRAALREIEEVEIEGTPNVDDGRWRRRVKAAEDMLAECRSAMGNMEKELQSKQKQIVQLRSKGQGGFGSQASSDQQRVEMHNMERKLASEVSRRMQTEKQLETLSLRLKEAETSLEQTLAVNAALPPDNSPLDYDPTKVKVVHLADNPLTQAILDHAMGKSKEKTSSRQVDDGDVDMSDGANSEILQLKKHINELEKENANLTKNSKVGLRTKEIAMKKIGEVRSAVYNLLGWSMKMAGAKYTLGSIYAESPNEILEFGINENGTFALMQNHYTNQLTEEIEQYVRKMDSVPSLLAQITMENIEKTTLMNV 745          

BLAST of Gcaud9918.t1 vs. uniprot
Match: R7QTF9_CHOCR (Uncharacterized protein n=1 Tax=Chondrus crispus TaxID=2769 RepID=R7QTF9_CHOCR)

HSP 1 Score: 273 bits (697), Expect = 4.140e-77
Identity = 240/753 (31.87%), Postives = 391/753 (51.93%), Query Frame = 0

Query:   15 AWLRVSNTIRSLQSEIRVLQDPAVRSALQTPPSAPR------TPAELNAKIVSLRTELSHREQKLREEKEATLAARQRAARAEGELQALHRQNEETSTLHERAVSDATSRIEELETRLAEHEASVRMHRVRQ---RDERERGRISDTSFASEREMLTTQAKVAEAKAKRLERELVAEKDRNVAEQKELQDKYDWAYGYAKRLQKEIDHLSNTRSSFGEF-RDANTSDLRKKNVSLESEVFRMRSQLRDESVNRTRMGARLAEMGAENDRLRELCRNAQQNEEQSKYMNEEITLLRIQASEEEQAVRNASALQSEKEELARLISALSPTGDAQEGIQILR-SLANGAGGNKSSDIAQRIAAQKY-EVQIAEIEEQHSI-----KLLRLQDELDDCRRIKSEMRNENIRLKEDKEAASAQQKRTELGRRILQREIDHLRAALREIE-EVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIE-------ELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVEKKHKEREHLDK-DVDMCDEG--NSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGAKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTT 739
            A L     +R L  ++ V +DP V+  L  P    +      +PAEL+A I++LRTEL+   ++ +  KE  L AR+RAARAEG++  L +Q  +    H+  V  A++RI+ L   + EH +++   R +    ++  E+  +   S  +E++ L  Q K AE+K   + RE+ A ++R+  E K      + A+     LQ E++ L  +      F ++  TS    K + +E+    ++S+L  +           A   A  D  +E  +N Q    +   + EE   LR    +      + SAL  EKEEL  +I  LSPT D QEG++IL+ S  +G    K +   + + +QK  E++    + Q+ +     ++ +L+  L+D     ++MR     L  D   +S   KR E  R I++ E    + AL +IE + +   ++     RLR+R +  E    +YKS L  +E  L   R+  E       +  S   G+ + +T ++ R  ++ +   +++S    + +  KK +        ++  +++L+      +   ++ D+DP   K +H+  NPL +A++    +   K  K+R  LD  D+    +G  +S +  L+ ++ +L+ +NE L+K SK+G+R  E+A KKIEEVR AVYNLFGWSM + GA Y I+SIYAE P ++LEFG NE+GT  LME  YT++L EEIEQYVQKMNS+P LLA IT+EN EKTT
Sbjct:    9 AVLEAREALRKLSGDLAVYRDPEVQRHLLNPALGSKRHLDSSSPAELHALIINLRTELNQAAEERQRTKEDILNARKRAARAEGQIDELQQQLTQKEVEHDEIVEQASTRIDAL---VKEHRSALLAARDKAFLLQETLEKDHLGHKSIIAEKDRLRIQCKEAESKLDGMRREIEATEERHAIEMKATVGDMERAWRKVDALQNEVERLRKSHGHTSGFSKEVATSRTLAKKLQIENAS--LKSELASQXXXXXXXXXXXARAKAAADDTKERIQNTQSERAELLALREEKRSLRDLRRKSANFEDHLSALAREKEELTGMIRDLSPTCDVQEGLEILKASKFDGLAKGKMTIAERNVLSQKNRELENTAKQSQNDLARQAKQIAQLKKSLEDAAAKLTQMR-----LARDNSLSSG--KRHERMRGIIEYEKSFFKQALEKIETDFDKPQSTDQYAERLRKRAEMYEKSCEKYKSELKELETALNEHRRNAEGTAKGLIKASSEWPGDGKVVTDAEGRGPEITKALQKMKSTIDAQEKVIKKSEMFEKRAKELEAEVQRLRK-----VQTETEFDYDPAVAKVVHMKSNPLEQAVKRAQEEEQIKAGKKRMRLDVGDLSPGRDGALDSRILGLQKEIRELESKNEDLAKKSKLGIRLGEVAKKKIEEVRAAVYNLFGWSMNVYGANYRISSIYAEGPRDLLEFGMNETGTMTLMETEYTSRLAEEIEQYVQKMNSVPALLASITIENFEKTT 744          

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A7S1XEL5_9RHOD (Hypothetical protein n=1 Tax=Compsopogon caeruleus TaxID=31354 RepID=A0A7S1XEL5_9RHOD)

HSP 1 Score: 112 bits (279), Expect = 2.820e-22
Identity = 96/330 (29.09%), Postives = 164/330 (49.70%), Query Frame = 0

Query:  425 ILQREIDHLRAALREIEEVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIEELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEK--LQAEHATL-LPDTSDP--------DFDPTEVKALHLVDNPLAKA----LRDYAMKTVEKKHKEREHLDKDVDMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGAKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTT 739
            ++++E++ LR  +    E E  G S  +   L  R+  +  +   YK  +  +E E+E+K  +   L S+ + E E L  +  +     ++   L+  A    E  +  +K+N    +    +E   L+ E A + L D +D         +F+P   K LHL   P   +     +    ++  +   E+  +   V +     +      T++S+LQ +   L++  K  LRT+E+A  KI+E+R A YNLFGW M ++GA Y++ SIYAE  +E L FG +  G   LM+  Y  +L  EI++++ +M SIP LLAH+T EN +K+T
Sbjct:  179 LMKKELEGLRTIISSFAESE--GVSAEESLALLSRVDRSVSLEG-YKQIINELESEVESKDLEGARL-SKRISELEQLLDNTEKTMDREKILSALKCVAKLTEENAELRNKINSQTTDKFAEVENSILRLEQANIELKDLNDRLARRLDAGEFNPQTTKVLHLKSRPPTSSGNLSRKRARSESAPESRAEQTPISNAVGLPPVVVAPAAGSVTELSELQTK---LAEAEKKALRTREVAKMKIDELRLACYNLFGWKMNISGATYSLISIYAERESETLTFGLSSRGGMELMDTPYCRELEPEIQRFLHQMRSIPSLLAHVTTENFQKST 501          

BLAST of Gcaud9918.t1 vs. uniprot
Match: UPI0018CE2D7E (mitotic spindle assembly checkpoint protein MAD1 isoform X1 n=2 Tax=Teleopsis dalmanni TaxID=139649 RepID=UPI0018CE2D7E)

HSP 1 Score: 90.5 bits (223), Expect = 4.890e-15
Identity = 88/312 (28.21%), Postives = 146/312 (46.79%), Query Frame = 0

Query:  455 RLRRRLKATEDVSSEYKSALASIEKELEAK-------------RKQIEELRSRSVGEFEPLTSSDPRLSKVREL---EHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAE--HATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVEKKHKEREHL---DKDVDMCDEGN----SELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGAK--YTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTT 739
            R++R+L         YK  L + +K+L                RK++E L     G  E  +  +  LS  ++L   E QLES        +K++D L +D   ++R  E+L+ E  H  L       DF+    K LHL +NP ++A + YA   +EK   E E L   +K ++   EG     +E  ++   + +L Q   +L  ++    + KE       E R  VY +FG+ +   G    YT++S+YAESPN+ L F  +++    L+E  Y+  L + IE  +    S+P  L+ +T+E  ++TT
Sbjct:  428 RIQRKLLLVSKERDCYKQLLENFDKDLTMSNTCVEVTNLETQLRKRVEMLEKTLAGYKEEFSKLETELSNAKKLPQSEQQLESGDAGYEYMKKELDNLRLDNEKLRRRKEELELELEHRCL-----KGDFNVDRYKVLHLENNPASEAYK-YADNLIEKLQAEIERLKRRNKKLEDDSEGTQSRLNETNSMTMNIKELNQLRAELESNNHKMKKLKEQYKAVNSEFREVVYMMFGYRVDRIGNNGCYTVSSMYAESPNDYLNFIFDDTSNLKLLETPYSITLKDSIETLLIGNKSLPLFLSSLTLELYDRTT 733          

BLAST of Gcaud9918.t1 vs. uniprot
Match: F6HK02_VITVI (Uncharacterized protein n=2 Tax=Vitis TaxID=3603 RepID=F6HK02_VITVI)

HSP 1 Score: 79.0 bits (193), Expect = 1.910e-11
Identity = 101/407 (24.82%), Postives = 191/407 (46.93%), Query Frame = 0

Query:  354 SSDIAQRIAAQKYEV--QIAEIEEQHSIKLLRLQDELDDCRRIKSEMRNENIRLKEDKEAASAQQKRTELGRRILQREIDHLRAALREIEEVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIEELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLV-----DNPLAKALRDYAMKTVEKKHKEREHLDKDV--DMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAG---------AKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQ--LLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            S D+  + AA + EV   + ++ E ++ +L +++  LD     K     E    KE  E + ++ KR EL   ++  E D LR A+ E+++              ++ ++A ++  +   + L   E  L  K   I+EL +    + E    ++ R ++++ L  +L +EA R              + +++R  ++L++E + L       DF  T  K L +V     DN   + +   A++T  +K KE+    +++     D G    + +  K+ Q +++   L K  +   R K +   +I   R A   LFG+ + +            ++T+ SIYA+S +E LEF   ESG   ++ N YT+Q  + +++E ++QK+NSIP   A++T+E+  K TL
Sbjct:  350 SDDVPMKFAALQKEVIEGMMKLGEANA-RLKQMEVSLDAAEHAKQNAETEAASAKESSEVSKSEVKRIELMLGLVTEERDQLRNAINELKK--------------QKNVEAGDETKNG--TLLQEFELSLAKKENCIKELENNLCEQKEV---NNRRFNEIKLLNEKLNNEARR--------------IKSLEREGDRLRSEISLLESKLGHGDFSATNTKVLRMVNTLAVDNEAKQTIE--ALQTELQKAKEKLEAIEELKTQSADSGKLVDSYVAGKIVQFKEQIATLEKREE---RYKTVFADRISVFRRACCELFGYKIVMDDHQRPNGIPVTRFTLQSIYAQSDDEKLEF-EYESGNTNILANAYTSQPEISQQVEIFIQKLNSIPAFTANLTVESFNKRTL 716          

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A0B7A767_9EUPU (Uncharacterized protein n=1 Tax=Arion vulgaris TaxID=1028688 RepID=A0A0B7A767_9EUPU)

HSP 1 Score: 75.1 bits (183), Expect = 1.080e-10
Identity = 52/187 (27.81%), Postives = 93/187 (49.73%), Query Frame = 0

Query:  565 DFDPTEVKALHLVDNPLAKALRDYAMKTVEKKHKEREHLDKDVDMCDEGNSELTTLRTKVSQ----------LQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGA-KYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            D+DP++ K +H   NP A A +    + ++K  +E E L K   + +E    +  L  +V +          +++   QL ++     R  E+  K  +E R   Y L G+ + +  A +Y + S+YA+ P +   F +N  G   +++  ++  + + IE Y+QK NSIP  L+HITME   + T+
Sbjct:  184 DYDPSKTKVVHFTLNPCALAQKARC-EELQKLKEENERLTKRNQILEEHGGNVEDLTVQVQEKLLHPSPSKEVEEMRAQLQREEMRNKRLMEVFKKTSQEFREVCYQLTGYKIDIPCANQYRLTSMYADCPRDHFLFQQNSQGEIQMLQTDFSLTVQDAIELYLQKHNSIPLFLSHITMELFGRQTM 369          

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A034VD87_BACDO (Mitotic Spindle assembly checkpoint protein MAD1 n=9 Tax=Tephritidae TaxID=7211 RepID=A0A034VD87_BACDO)

HSP 1 Score: 71.6 bits (174), Expect = 3.650e-9
Identity = 77/298 (25.84%), Postives = 130/298 (43.62%), Query Frame = 0

Query:  453 DGRLRRRLKATEDVSSEYKSALASIEKEL-EAKRKQIEELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKA------LRDYAMKTVEKKHKEREHLDKDVDMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAG--AKYTIASIYAESPNEVLEFGRNESGT-FALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            D +LR RL   E     YK   +++EKEL  AK+  + +    +V   E        L  +R     LE+E  RR + E                  +L+ EH  L       DF+  + K +HL  NP A+A      L +     +E+  +  + L++D ++     +E T +   + ++ Q   +L   +    + KE       E R   Y LFG+ +   G    Y ++S+YAESP++ L F  NES     ++E  Y+  L   IE  +    S+P  L+ +T+E  +++T+
Sbjct:  462 DVQLRSRLDMLEKTLVGYKDMCSNLEKELTNAKQLPVADQSIENVISTETYEHIKKELDTMR-----LENERLRRRKEEL-----------------ELELEHRCL-----KGDFNVGKFKVVHLAQNPAAEAYENSNNLIEKLQAEIERLKRRNKKLEEDNEVTQARMNETTNMTINIKEMNQLRAELEATNSKMKKMKECYKSASMEFREVCYLLFGYRVDRLGLNTNYKLSSMYAESPDDYLNFRLNESNNALDMLETPYSITLKPFIESQLVGNKSLPAFLSALTLELFQRSTM 732          

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A0K9S482_SPIOL (Uncharacterized protein n=2 Tax=Spinacia oleracea TaxID=3562 RepID=A0A0K9S482_SPIOL)

HSP 1 Score: 70.1 bits (170), Expect = 1.080e-8
Identity = 117/475 (24.63%), Postives = 213/475 (44.84%), Query Frame = 0

Query:  289 KYMNEEITLLRIQASEEEQAVRNASALQSEKEELARLISALSPTGDAQEGIQILRSLANGAGGNKSSDIAQRIAAQKYEV--QIAEIEEQHSIKLLRLQDELDDCRRIKSEMRNENIRLKEDKEAASAQQKRTELGRRILQREIDHLRAALREIEEVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIEELRSRSVGEFEPLTSSDPRLSKVRELEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVE----------KKHKEREHLDKDVDMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAG---------AKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQ--LLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            K  +E I LL+ + +EE++      A  ++ +E+ R ++ L     + +  ++++ +    G   S DI  +  A + EV   +A++ E ++ +L +L+  L+  +  +  + +E    KE +E+     K   L    +  E D LR A  E+++       +P +  + R L             +  +E  L  K   I+EL    V   E   ++D   +++R L  +L  EA R              L +++R  ++L++E A L       DF     K L +V+   A  +   A +T++          +K K  E L K     D G    + +  K+ QL+++   L K  +   R K +  +KI   R A   LFG+ + +            ++T+ SIYA+S +E LEF   ESG   ++ N Y  Q  + +++E +VQK+NSIP   A++T+E+  + TL
Sbjct:  293 KASHENIELLKEKFNEEKRRRERVEAELAKLQEIQRSMNVLEDELTSWK--KVIKDIP---GVESSDDITLKFVALQKEVIGSMAKLGEANA-RLKQLEVALEGAQLDRKNVESETALAKEKEESFKLDIKELNLKLLSVNEERDQLRTAFDELKQQT---NIEPGNEVVGRAL-------------VQGLESCLAQKDNYIKELE---VNLSEQKAANDRHHNEIRMLNEKLNDEARR--------------LKSLEREGDQLRSEIALLESKLGHGDFSAANTKVLRMVN---ALGVETEARQTIDALQCELQKANEKLKVVEELKK--QSADAGQLVDSYISGKIVQLKEQIATLEKREE---RYKTVFAEKISVFRRACCELFGYKIVMDDQQRPNGIPITRFTLQSIYAQSDDEKLEF-EYESGNTNILVNDYAKQRDISQQVEIFVQKLNSIPAFTANLTVESFNRRTL 719          

BLAST of Gcaud9918.t1 vs. uniprot
Match: A0A835FV08_9POAL (Uncharacterized protein n=8 Tax=Paniceae TaxID=147428 RepID=A0A835FV08_9POAL)

HSP 1 Score: 69.3 bits (168), Expect = 1.870e-8
Identity = 154/625 (24.64%), Postives = 276/625 (44.16%), Query Frame = 0

Query:  175 LERELVAEKDRNVAEQ----KELQDKYDWAYGYAKR-------LQKEIDHLSNTRSSFGEFRDANTSDLRKKNVSLESEVFRMRSQLRDE---SVNRTRMGARLAEMGAENDRLRELCRNAQQNEEQSKYMNEEITLLRIQASEEEQAVRNASALQSEKEELARLISALSPTGDA------QEGIQILRSLANGAGGNKSSDIAQRIAAQKY----------EVQIAEIEEQHSIKLLRLQDELDDCRRIKSEMRNENIRLKEDKEAASAQQKRTELGRRILQR--EIDHLRAALREIE-EVEIGGTSQPDDGRLRRRLKATEDVSSEYKSALASIEKELEAKRKQIEELRSRSVGEFEPLTSSDPRLSK-VRELEHQLES--EACRRHEAEKKV--DKLNVDLNNV---QRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVE------KKHKER-EHLDKDVDMCDEGNSELTTLRTKVSQLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGW---------SMRLAGAKYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQ--LLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            +E+EL A K R  A Q    KEL D  +  +   K+       L KEID      SS    +++   DL  K   L     R +  LR E         + A   +   E  RLR     A   E+++K +NE++  L+ Q  E    +R  + ++ +    + L    +PTGD       QE ++      + A   KSS     +  +K           E ++++++E  + K  +L+ EL  C  + S        + +    A   QK  E  +  L    ++  + + L+E+E  +E    S       ++R +    ++ E     A   K LE     I E R++   E  P+ S    + K +RELE  ++   E     +AE  +  ++L+++   V   +R  ++L+++ A L       D+  +  K L +V N LA  + + A +T+E      KK KER + +++     D G      +  K++QL+ +   L K  +   R K +  ++I   R A  +LFG+         S  +   ++ + S+YA+S +E LEF   ESG+  ++ N YT+Q  + ++++ ++++MNSIP   A++TME+  K ++
Sbjct:  124 VEQELAATKGRESALQERLLKELADYQERYHDQVKKINELEVQLNKEIDSRIRAESSASSAKES-IKDLEGKLQRLSESSEREKKTLRKELSFMKEDLTLSASKLKTELEKTRLR-----ADNYEDEAKLLNEQLVDLKRQLDE---CLRKKNEMELKSLNSSALPDQRAPTGDQTLIKLLQEELRNYEKEVHEAKRLKSSHTNAELLKEKLLEEQGRRERAEQELSKLQEIEA-KAHKLELELASCTALLSN-------IPDVSSYADIPQKFAEFQKEALTNLNKVGEVTSRLKELEVALEFADLS-------KQRAEGEATLAKERAENAAKEVKRLELMLAAISEERNKLRKEH-PMESDQSGMEKFIRELESTIQELKELISHKDAELNIMNERLSLEARKVKSLEREGDQLRSQMALLESKLGHGDYSSSSTKVLRMV-NTLA--VDNEAKQTIEALQAELKKTKERLQAVEELKGQADVGTVVDANIAEKLAQLKNQIATLEKREE---RYKAVFAERISVFRKACCSLFGYKIVMNDQQQSNGIPVTRFILQSVYAQSDDEKLEFDY-ESGSTNIVVNDYTSQQEIAQQVDVFIRRMNSIPAFTANLTMESFNKRSI 716          

BLAST of Gcaud9918.t1 vs. uniprot
Match: UPI001ED88B29 (mitotic spindle assembly checkpoint protein MAD1 n=1 Tax=Ischnura elegans TaxID=197161 RepID=UPI001ED88B29)

HSP 1 Score: 67.4 bits (163), Expect = 7.900e-8
Identity = 81/327 (24.77%), Postives = 150/327 (45.87%), Query Frame = 0

Query:  459 RLKATEDVSSEYKSALASIEKEL---EAKRKQIEELRSRSV-GEFEPLTSSDPRLSKVRE--------LEHQLESEACRRHEAEKKVDKLNVDLNNVQRTMEKLQAEHATLLPDTSDPDFDPTEVKALHLVDNPLAKALRDYAMKTVEKKHKEREH-------------------------LDKDVDMCDEGNS-ELT-TLRTKVS-----QLQQENEQLSKDSKVGLRTKEIAMKKIEEVRGAVYNLFGWSMRLAGA-KYTIASIYAESPNEVLEFGRNESGTFALMENHYTNQLLEEIEQYVQKMNSIPGLLAHITMENVEKTTL 740
            R++  E +  +Y+  + ++E EL   + KRK IEE  S  V G++    S +  L K++E        LE +LE     + EA    ++L+  ++ ++  +     EH  L       DF+P   K +H   NP A A   Y  +    K                               +D D+  CD+ ++ E+T  +  ++      ++++  EQL +   +  R KE    KI++ R A Y LFG+ ++L G  ++++ S+YAESP + L F  + SG  +L+E  ++  L + I  ++ + +S P  L+ +T++   + T+
Sbjct:  491 RVEELELIIEKYQKNMETLEAELIEEKKKRKAIEEANSVMVTGKY----SEEDMLRKIKEAVDDEKAKLEKELEVLWKEKEEARXXAERLSAHVDQLEGEL-----EHRAL-----KGDFNPKGTKVVHFKMNPAALASEKYVDELEAYKXXXXXXXXXXXXXXXXXXXXXXXAQGSSTIAMDGDLLPCDDSSALEVTKVVEARIQAQGSKEIEELREQLRQAKLLPERLKEAFKMKIKDFRDACYCLFGYKLKLIGVGRWSLYSMYAESPEDHLLFEISSSGLPSLLETPFSATLEDLIRIHLMRQHSFPAFLSAVTLDLFNRQTM 803