mRNA_F-serratus_M_contig88.20165.1 (mRNA) Fucus serratus male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-serratus_M_contig88.20165.1
Unique NamemRNA_F-serratus_M_contig88.20165.1
TypemRNA
OrganismFucus serratus male (Fucus serratus male (Toothed wrack or serrated wrack))
Homology
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BLAST of mRNA_F-serratus_M_contig88.20165.1 vs. uniprot
Match: D7FV45_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FV45_ECTSI)

HSP 1 Score: 104 bits (259), Expect = 7.190e-24
Identity = 65/99 (65.66%), Postives = 79/99 (79.80%), Query Frame = 3 
Query:    3 MAFISPSTPMTCRGVARASARSRNSERG-LLMASRPNAKKEKRQRNRDNMRKFTKKGTSRKKLMRVQRQERAAEAESDFMAQVFHTGMDDWEEDDIFAE 296
            +AF++P T  T R  A  SARS  S RG  LM+ R NAKKEKR RNR NMRKF K+GTSRKK++RV+RQERAAE E++F+A+VFH+GMDDWEE DIF+E
Sbjct:   17 LAFVTPMT--TLRAPATTSARSTASARGGALMSCRVNAKKEKRTRNRLNMRKFNKRGTSRKKILRVERQERAAEDEAEFLAKVFHSGMDDWEEVDIFSE 113
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BLAST of mRNA_F-serratus_M_contig88.20165.1 vs. uniprot
Match: A0A835YKP5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YKP5_9STRA)

HSP 1 Score: 60.1 bits (144), Expect = 8.050e-8
Identity = 35/59 (59.32%), Postives = 48/59 (81.36%), Query Frame = 3 
Query:   75 SERGLLMASRPNAKKEKRQRNRDNMRKFTKKGTSRKKLMRVQRQERAAEAESDFMAQVF 251
            SE  L MA R NAKK+K +RN+DNMRKF K+GTS+KK++R +RQ+ A E E+DFMA+++
Sbjct:   37 SESRLSMACRNNAKKQKAKRNQDNMRKFRKRGTSKKKIVRGKRQQTAREKEADFMAKLY 95
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BLAST of mRNA_F-serratus_M_contig88.20165.1 vs. uniprot
Match: A0A835ZDX5_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZDX5_9STRA)

HSP 1 Score: 57.8 bits (138), Expect = 7.690e-7
Identity = 33/54 (61.11%), Postives = 45/54 (83.33%), Query Frame = 3 
Query:   87 LLMASRPNAKKEKRQRNRDNMRKFTKKGTSRKKLMRVQRQERAAEAESDFMAQV 248
            L MA R NAKK+K +RN+DNMRKF K+GTS+KK++R +RQ+ A E E+DFMA++
Sbjct:   42 LTMACRNNAKKQKAKRNQDNMRKFRKRGTSKKKIVRGKRQQTAREKEADFMAKL 95
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BLAST of mRNA_F-serratus_M_contig88.20165.1 vs. uniprot
Match: A0A7R9YX41_9STRA (Hypothetical protein n=1 Tax=Pseudictyota dubia TaxID=2749911 RepID=A0A7R9YX41_9STRA)

HSP 1 Score: 52.8 bits (125), Expect = 5.110e-5
Identity = 32/54 (59.26%), Postives = 42/54 (77.78%), Query Frame = 3 
Query:   90 LMASRPNAKKEKRQRNRDNMRKFTKKGTSRKKLMRVQRQERAAEAESDFMAQVF 251
            L A R NAKKEKRQRNR+NMRKF K+G SRKK+M+     +A +AE++F+A+ F
Sbjct:   62 LEACRVNAKKEKRQRNRENMRKFQKRGLSRKKVMKKAFSAQARQAENEFLAKCF 115
The following BLAST results are available for this feature:
BLAST of mRNA_F-serratus_M_contig88.20165.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef90)
Total hits: 4
Match NameE-valueIdentityDescription
D7FV45_ECTSI7.190e-2465.66Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A835YKP5_9STRA8.050e-859.32Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A835ZDX5_9STRA7.690e-761.11Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A7R9YX41_9STRA5.110e-559.26Hypothetical protein n=1 Tax=Pseudictyota dubia Ta... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-serratus_M_contig88contigF-serratus_M_contig88:520858..526900 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef902022-09-19
OGS1.0 of Fucus serratus male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score108.6
Seed ortholog evalue1.7e-21
Seed eggNOG ortholog2880.D7FV45
Hectar predicted targeting categoryother localisation
EggNOG OGs2E66M@1,2SCXT@2759
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-12_005120.1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons4
Model size1092
Cds size300
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-serratus_M_contig88.20165.1prot_F-serratus_M_contig88.20165.1Fucus serratus malepolypeptideF-serratus_M_contig88 521648..526898 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622932564.5006666-UTR-F-serratus_M_contig88:520857..5216471622932564.5006666-UTR-F-serratus_M_contig88:520857..521647Fucus serratus maleUTRF-serratus_M_contig88 520858..521647 -
1690964368.0794737-UTR-F-serratus_M_contig88:520857..5216471690964368.0794737-UTR-F-serratus_M_contig88:520857..521647Fucus serratus maleUTRF-serratus_M_contig88 520858..521647 -
1622932564.6359646-UTR-F-serratus_M_contig88:526898..5269001622932564.6359646-UTR-F-serratus_M_contig88:526898..526900Fucus serratus maleUTRF-serratus_M_contig88 526899..526900 -
1690964368.1337702-UTR-F-serratus_M_contig88:526898..5269001690964368.1337702-UTR-F-serratus_M_contig88:526898..526900Fucus serratus maleUTRF-serratus_M_contig88 526899..526900 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622932564.5291703-CDS-F-serratus_M_contig88:521647..5217821622932564.5291703-CDS-F-serratus_M_contig88:521647..521782Fucus serratus maleCDSF-serratus_M_contig88 521648..521782 -
1690964368.0912528-CDS-F-serratus_M_contig88:521647..5217821690964368.0912528-CDS-F-serratus_M_contig88:521647..521782Fucus serratus maleCDSF-serratus_M_contig88 521648..521782 -
1622932564.5559573-CDS-F-serratus_M_contig88:522236..5222781622932564.5559573-CDS-F-serratus_M_contig88:522236..522278Fucus serratus maleCDSF-serratus_M_contig88 522237..522278 -
1690964368.1018798-CDS-F-serratus_M_contig88:522236..5222781690964368.1018798-CDS-F-serratus_M_contig88:522236..522278Fucus serratus maleCDSF-serratus_M_contig88 522237..522278 -
1622932564.577552-CDS-F-serratus_M_contig88:523138..5231851622932564.577552-CDS-F-serratus_M_contig88:523138..523185Fucus serratus maleCDSF-serratus_M_contig88 523139..523185 -
1690964368.1123264-CDS-F-serratus_M_contig88:523138..5231851690964368.1123264-CDS-F-serratus_M_contig88:523138..523185Fucus serratus maleCDSF-serratus_M_contig88 523139..523185 -
1622932564.60017-CDS-F-serratus_M_contig88:526822..5268981622932564.60017-CDS-F-serratus_M_contig88:526822..526898Fucus serratus maleCDSF-serratus_M_contig88 526823..526898 -
1690964368.1235394-CDS-F-serratus_M_contig88:526822..5268981690964368.1235394-CDS-F-serratus_M_contig88:526822..526898Fucus serratus maleCDSF-serratus_M_contig88 526823..526898 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-serratus_M_contig88.20165.1

>prot_F-serratus_M_contig88.20165.1 ID=prot_F-serratus_M_contig88.20165.1|Name=mRNA_F-serratus_M_contig88.20165.1|organism=Fucus serratus male|type=polypeptide|length=100bp
MAFISPSTPMTCRGVARASARSRNSERGLLMASRPNAKKEKRQRNRDNMR
KFTKKGTSRKKLMRVQRQERAAEAESDFMAQVFHTGMDDWEEDDIFAED*
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mRNA from alignment at F-serratus_M_contig88:520858..526900-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-serratus_M_contig88.20165.1 ID=mRNA_F-serratus_M_contig88.20165.1|Name=mRNA_F-serratus_M_contig88.20165.1|organism=Fucus serratus male|type=mRNA|length=6043bp|location=Sequence derived from alignment at F-serratus_M_contig88:520858..526900- (Fucus serratus male)
CAATGGCATTTATAAGCCCGTCGACACCCATGACTTGCCGGGGAGTCGCG
AGGGCGTCTGCACGTAGTCGCAACTCAGGTACGAATATAATGACCTCTAC
CTCCACTACCTGTACTGGCTCACCTCCTACGCTGTTCCCGAATGGCATTA
GACTAGAGCATTCGTTGAAGTATTGGTGCGACAATACAAACTGGTTTCCA
AGATGTGTACGATGCTATCGCGATTGAGTACCAGGTAGCTACCGGCTGTC
ACTTTTCTACGGACGGTTCGTGCCATTCGATCGCTACTCTGTGAGAACCA
AGTTAAGTACAGCGGCAGTAAAATTAGTCAGGCGGGCATCTGTGAACCGC
GTATGAGCATGATCTTACATGTTACCGGTACAGTACTACTATAATACAAC
AGAAATGTGTTCGAGTGAACAAAATCATGACTATTATTGTACTTCGCACG
TTGTAGTCAGTACAGTACATTATTTTCATAGACTACAGAGTAGTATCTCC
GAAGCTCCCTGCTAACCAAACGGCGTTTTCATGGACATGTTTCTTTCGGA
GTGCGTGCGTACCGTTAGACAGCAGCGTCACGAAGAAAAGTGTCCTCATA
CAAGCACATACAGAGTTAGGTATCTGATCTTAGGCCAACCTTTATGGGAA
ACGAAAAAACAGAAACGCAAAATACAGAAATTTGTCATTTCTGTGGCCAA
AAAATCCGTGGGGAAAAACTGTAGTTGGTCGTTTATGGGATAAAGGAAAA
ATACCAATTGGAAAATACAAAAAAAAACGGGGAGCATATCGGGTTAACCC
CGGCATTTTCGGCCACCCATCACCGCACAAAGACAGGTGCAAGTATGTAC
ACATGGCGAGTATGGATCAACCGGGTAAGGTTGCCAGTCCTGTTCGTGGT
CAACTGAACAGGGAAAAATAATATTTCCCTCTCCGCGTTTGTGCCCGAGA
ATTTAGTCCGGCGAGACAGGGTCGTTTAGTACTTAATTACGAGTACATCG
CAAATAGAGTGGGCAGAGCGACATTGTTTCGATGGCGAGCCGTCGGACGC
TCGTGACACGCAGCCCGTCCCTGCTTGATTGATCTCCGGCGCCACCCATT
CAACCGCCCCCGCCGCCGGCGATCAGCTTGTCCAGGCCCATCACCTCAAC
AGCTTTTATAAATTTCCTCCAGATCTGCTTGCCCGGGCCCATCACCTCAA
CAGATTCTATAAATTTCCTCCAAATCTGCTGCTCCGGCGATCATCGCACA
ACTACTGCTGCTCAGCACCTATCGTACAATTTTCTTCAAGCACTCGCGAG
CTCTTCTTTGCCGATATTTGATAGGAAGGATGCTACAGTCCCGACCTCCC
AATCGTTCTTGGAAGGCGTGAATCATTTCTGCGGCAAACTAAACTTGTCG
AGCCACATACAGAAATGAGCCTCATTTCTGTATGTGGGAAGAGAAAAATA
ACCTCCCACAGAAATTCGCCATTTCTGTAGGGCTACATTTCTGCGGCACG
GGGCCCATAGCGCCACGTTTACAAAAAATTCGCCACAGAAATTTCACATT
TCTGTATTTTCATGTCGTTTCTGTTTCCCATAAAGGTTGGCCTTAGGCCG
GATCGGTTTCAGGTCCAGCTTTTGGACTGACTTGTTCATGTATAGTCTAA
TTGTATGTAGTTTCACGCTTCTCGATGTATTTCTCACTGAGAAATTACCA
TTATGTCACTTTGTAAGGCCCTATATGGCCACACGTGTAGCAAGAGTATG
GATCAACCGTGTAAGGTTGCCAATCCTGCACGTGGTCAGCTGAACAGGGA
AAATGGATATTCCCCCGTCCGCGTTCGCGCTTGAGAATTTAGTGTCGCGA
GATGGGTTCGGCAGTCCCGTCCCGCGTCAGCCTGCTCATCTTCATACTTA
GGCTGAATCTGGTACTTACTTGCGGGATTCCTCACGAGTTCCGCGGCGGC
GTCCATTTATTCATTTAAAGCCGCCATACACCATCGGGTCAGTCCCGAGT
TTACCGGGTCACGCAATTGCGTACCAATGGCGTTCACTGCCGAGAGTCCG
CCGGCACAGGGTCAGTAACCTCAGATAGTTCCGAACGAGTGCTGCCTTGG
CAGGTCACCGTGGACGAATTAATATACGCCTCTCTTTCCCACACGCACTA
TTGGCATGAAGTGGGCATGTTGAAAGTATCGGCGACATAATATAATTGAG
ACTTCACCCCAGGGAAATCATCATCAGGGGGGAAGTCCTCATCCGCCGAT
GAAAACTTTCCGTAAGTTCTCATCGGCTAGCGATAATTTCTTTGCTTGAC
AATAATTTTCTATTTGGAGTTTGTACCCAAGTCAAAACTCGATGGAAACC
CATTTCATACGAGGTCCCTGCTGGCAAACTGGTGGGCCGTGAGTACGATG
TCAATCACCGGCAAATAGCGCTCATCACGAGTAAAAGACTTTAAAGGGGA
GGTAAACAGCTCTTAATGCTATGTATTGGTAATAATAGAGCATGCTGGTG
GGTTCACTCGATGGTAGCGAAAAGACTGCGAGGTCGAAGCAACCCCGAGT
TGAGCACAGATAAGTGGCATGTCGCCCACCCCGAACTACTAAAAATATGG
TGTAAACTCATTGGCGCGTAGAGAGTAGTTTCAAGCTCGAGGATTCCGTG
TCCGTTTTTCTGTTTTCTCAGGAGATCGTACAAGTCAAATACAACACCTG
ATGAGAGACAGATCCATTTGCAAAAATATGTGCCGTACCTGGATGGATCC
AAAGAGCTCGGAATTGAAGGATTGAAGGCTTACAACACTTTCCCGGTGCT
GCAGTATGTCGGGTCGGGGGCGCTGTGCGTCTGTGCTCGGTTTGGTAGAC
CAATAGACCCTACTGCTGCATCGCCGGTACCGCTACTGTTTGTGAGCAAT
TCTCCCTGCCGTAAACGATAGCATTGGTACTGCTGTGTGCGCTATAAAGT
CGCAAATATTAGTATAAAATTCCCTTTCTTTTCATGGGATGGAATCTCCC
GTTGCTATGACAGTCAGTTTATACTGTACGAGTAATGATTTTGTATCCAA
AAACGGAGCGTTGACGGTATAAGTAGAAGGACGATAGCAAAGTGTTTTGA
GTTAACAGTTGAAAACAGGGTAATCATCTGACATAAGTCGGTGGACTCGG
TGTGAGCTATGGGAAGACGCAGCGGTCACCTGTGGCGCAGGGCATGTCTG
CGAACACACACCCTCAGCAGCACACTGCCGCTGCACGGAATTGCTCGTAG
GCGGCAAGTTTTCGGGGGGACCGTAGGTTGTCTGCATCATAGACTTGAGA
GACCGCCTGGCATGACGAGGAATGGAGGGCTCTGATACCGTAAAAACAGC
AAATGACTCCTCTCCCTGCCTCTGGGTCGCTAATATTTGATATCGTCCGC
GTGTTTTGTGTTAAAATTACTCACTACTTCGGAGTATTATTAAGACGCGC
TAAAACCTACCTGATTCAATCCCAAGGAGAGTTTTTGACCTTGTAGCCAA
TGTTCCTATCTAAACATCTTGATAATTCCCCCCCCTGACTAGACGAATGC
TCAGAAGGTTTCCATGCGTTTTGTTTTTTCTTGAAATTAAACCTTGTAGA
GTCCTGGCTCCTACTATCCCGTCCTCTTTTTCTGCCAACGTTTCTCTCGT
AACTTACCGGTGCCGTGCTGGCGGGGATGGGGTATCGTGGCGCGGCTTTG
GGGGGGGTGTCACAGAGCGCGGCTTGCTCATGGCTTCCAGGCCTAACGCG
AAGAAGGAAAAGGTATACCAGGGTATCCCGCGAACGTTCTGTACCTAAAT
AAACCTCTTGGGTCGTAACTTTTTAGCCCTCGAAAGAATCGACCAGATAC
GTCACGAAGCTAAGTCCCGCGTGTACCTAGTGGGTATGTCCATGTAGTTA
TTCATCGTTGAAGTGCATTCATTATAATAGCAACAGCGCCTATGCGCGCG
TTATCAGGTGACTTTTTGTGTTTAAAGAAAATTTGTTTTGTCAAGGTTAG
CAGAAGTTGTGAGCTGTTTTTGAAAACGGATTGAGCTGCGGCAACGTTTT
CGAACGTCGGCATCACGGTCCGACCTGAGAACCGATTTGACAAGAAAATA
TTTGTGTTACGTCGTTGTCGTGTGAATTAACCCACACCACGGGCTACTTT
GAAATATCTAGGATGTGGTGTGAGACGTTTCCCTAGCACTTGTCTATGTG
TTGCCTTGTCGCTTGATTATGTTTATATGCCTCGGTCAATCACGGTCCTC
GAACGTCTTTCTTGGCGAAAGTTCCGTAAGCAGTGAAAACATTTTTCAAT
ACTTGCTGTTGAGTTTTGGTCGCTGGAAGGACATGTTAGCGAGTTTTGCC
ATGGGGTACGAGGACATCGGTCATCCTCAGGGCGCAGTCAAAGCAGCCCG
ATTGTGCGTCGAGACAACCATGTCGTGTGTATAACATTCGACCCGGGGCT
CGCATATCAAAAAGAGTTGTGTAGGGCCTGGACACACAAAAAAATAGCCC
TTTCTAGCTTTTGATATTCGCGTATTCCTCTCTCTCCACTCTCCACTCTC
CACTCCCTCCGCGTCGGTGGTTATGTGATCGTGATCGTCTGTCTATGTGA
TCGTGATCGTCTGTCGACGCAGAGGCAACGCAACCGCGACAACATGCGCA
AGTTCACCAAGAAGGTGAGTCAACCGCACACTGTCCATACTGCAATAGAT
CCAAGTCGCCTCTTTCTTACCGAAGATAACAGAGCTCTAGGGCTGCGCGG
GGCCAATTTATTCCGTTCCGGAGTCTATCGTTTCCCAATGTTTGAGACAA
AAGTGCCTACTTTGAACTATCAAAGTGGAGATTCGTAGAGATAAATACGC
GGAACTCGGCTTGAACGAGCGATGGCAATCCCCCCCTGACATCGTGCGTC
TTTTGAGCATCCCTCGCGGTGACTCCGAAGGGCCTTTACGATATTGAGAC
ATCAGCGGTAGCACGCAAGACTGCAAACAGAGGGGATGCGAGGAAACGAT
CTATGCCTTGAAAGCTGCTTGCGCGAAGGATCACGAGTCAGCGTTCGCTT
TGCCTCATGTACTGCGCGCTCAGACCTTGGGATGCGCTACCCTGCATTGT
ATTTTATTGCCACCTCAGGGAACGTCGCGAAAGAAACTGATGCGCGTGCA
GCGCCAGGAGCGTGCCGCAGAAGCGGAGTCCGACTTCATGGCCCAGGTGT
TTCACACCGGGATGGACGACTGGGAGGAAGATGACATCTTCGCCGAGGAT
TGAGTGTTCTCGAACTCTTTGTTTCAATCCACGTTGCGTGTGGTATCCAT
ATGCTTCCACGCCAGGAGAGTACGAGGGGACTTTTACGATATTCTTGGCT
TGCGTTTCTATTAATAGAAGGCAATGATGGGATTCAGGGGGCCATGGACC
GGCGTATTCTCGGGCGAATGGGATTATCGCACTAAAAAGCTTGGATTGTT
GACGTCCGCGGCATGTTAAATGTGGCAGTTTGACAATTTAGCTCCCAACA
ATAGGAGATGGCGAAGTATTGAGGATTGGCTGTGATTGTGAAAATGTTAG
AAGGACGAAAATCGAAATTGGCCTGTGTACATCTGAACGCCGAGAGTTCA
TGTTGAGTCAGAATCTGATGCTCCACGCTGCTAGCAGGTCACATGCGACA
ACACGGTGATGGAGCAAATGCGACAGACAGGTTCTAATACACTCGACATT
TTCCGAAGTGATAGATAAGCTAAGGCGAAATATGTAGCACCAACATTCGG
GGAATGTAAATGTCGCGATGTCAAAATGCACGTTCTGCTTTTCGCCAAAG
TAGCCTTTGGGTTGAGAGAGTACCGGTAAAATTCCCTGCCGCAGCTCGGA
CGTCGGCGTAAAATGTTCAAAAACAAGAAAAACCTTTACCCTTTGAATTA
TAAATCAATGCACAGTGCAAAACAAGTACTGGTAGACAGTATCAGCTATG
TCGTGTTCTTTGTGAGCGCGAGCGCCGTCTGCGCCGGAAACCTTCACAAC
AAGATAATAAGATAAATTGCGCGAATACGAACTATTAAAAGTA
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Coding sequence (CDS) from alignment at F-serratus_M_contig88:520858..526900-

>mRNA_F-serratus_M_contig88.20165.1 ID=mRNA_F-serratus_M_contig88.20165.1|Name=mRNA_F-serratus_M_contig88.20165.1|organism=Fucus serratus male|type=CDS|length=600bp|location=Sequence derived from alignment at F-serratus_M_contig88:520858..526900- (Fucus serratus male)
ATGGCATTTATAAGCCCGTCGACACCCATGACTTGCCGGGGAGTCGCGAG
GGCGTCTGCACGTAGTCGCAACTCAGATGGCATTTATAAGCCCGTCGACA
CCCATGACTTGCCGGGGAGTCGCGAGGGCGTCTGCACGTAGTCGCAACTC
AGAGCGCGGCTTGCTCATGGCTTCCAGGCCTAACGCGAAGAAGGAAAAGA
GCGCGGCTTGCTCATGGCTTCCAGGCCTAACGCGAAGAAGGAAAAGAGGC
AACGCAACCGCGACAACATGCGCAAGTTCACCAAGAAGAGGCAACGCAAC
CGCGACAACATGCGCAAGTTCACCAAGAAGGGAACGTCGCGAAAGAAACT
GATGCGCGTGCAGCGCCAGGAGCGTGCCGCAGAAGCGGAGTCCGACTTCA
TGGCCCAGGTGTTTCACACCGGGATGGACGACTGGGAGGAAGATGACATC
TTCGCCGAGGATTGAGGAACGTCGCGAAAGAAACTGATGCGCGTGCAGCG
CCAGGAGCGTGCCGCAGAAGCGGAGTCCGACTTCATGGCCCAGGTGTTTC
ACACCGGGATGGACGACTGGGAGGAAGATGACATCTTCGCCGAGGATTGA
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