prot_F-serratus_M_contig872.20087.1 (polypeptide) Fucus serratus male

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 909 bits (2349), Expect = 4.990e-316
Identity = 515/1017 (50.64%), Postives = 671/1017 (65.98%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGS-GKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP---GVSSVGPLLPSLKLAPSLLDHGFDQG--LGDISDVPGTLNLAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADPEVVHNDGRSTRLSREWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPITSISADGEETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEV---VDSGLDETSCFFSDDDGLEIDHGHLYHGLRSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLE--GDWWEYLSSST-WSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETNGLFNIDTFCPAYDCDGTSE-NSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDI-GFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAAIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            F+     +  AS W++ RL    + G   PY+ CA    GR A  SLE   + SA+H +S+T+ DG+CF+ +  P+   +V+RAP    + S  PLLPSLKLA SLLDHG D    LG   D   + + A PL++ +G  +   +  GLS+R+ PGVL   G  S A+  +R+W A+L S +V++   S+W+D +   ++   TR+ REW RAA VV  LA+    T GE CGF ++++ H  DDL+L EG++HLLP     +   ET+ AC+MGLLSQLAAK EVLRV+P   A + NAVA+A+ QS+T +ETPL DAG+DGTGEV   VDSGLDETSCFF+DDDGL+++HG L+ G+R + DG++ T  GNY       FP++M+RRK+VQY+++F T +  +  S    D  T  S+Y   LG  Y   D+          D    +   YS  Y   +W S    C   L+     + YL   T +SS  TS   +GGFHKD   GHGT  AG+A G+ S  S    + C G    P CAGGC+S+S+ + +  +  F++D FCP Y CDG ++  SS CL +D ++ L  HGGVAPGA+I+ FD S+ G  + + +AGN LW S++ TGAK+H++SWG  T CQL E E+ YDTFMYENPE+LLIFAAGN GG +DI   E CTV SPALGKN L+VGATS+G      TG DG   YEE GL+DYSPEG PWICL PFLG PS +++PA  DTV+FFSS GPT DGRIKP++VAPG++V S SSDG++ HSC+L++  GTS + P+VAGAAALVRQYF+DPSF+ KD+ +RG C E SSS   C+ F+P+AATVKAM+INSA+ MGGS+ P+  RGFGRVHLEAGMP++G     L V EAAI A+ E    V +D  AG+ELRATL W+D PT ALS KQLQHDLDL V+AP+G  YTMW +G  D VNV ERV++P ES+ESG +SV VS   LLTD   YSLVVT AI
Sbjct:   29 FARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSA-PLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRV-REWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPS---ETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNY------SFPFDMSRRKIVQYIQLFKTPDQGSTSSGQ--DSFTYVSDY---LGDPY---DYD---------DSEYISYDDYSGEYYSDTW-SEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGD-ELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSC-QCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAI-ADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 1013          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 851 bits (2198), Expect = 7.110e-294
Identity = 491/1018 (48.23%), Postives = 632/1018 (62.08%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP---GVSSVGPLLPSLKLAPSLLDHGFDQG--LGDISDVPGTLNLAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADPEVVHNDGRSTRLSREWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPITSISADGEETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEV---VDSGLDETSCFFSDDDGLEIDHGHLYHGLRSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLS-----SSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETNGLFNIDTFCPAYDCDGTSE-NSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDI-GFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAAIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            F+     +  AS W +KRL     G   PY+ CA    GR A  SLE   + SA+H +S+T+ DGSCF+ +  P+   +++ AP    + S  P LPSLKLA  LLDHG D    +G   D     +L+ PL++ +G   +Q                                                            TR+ REW RAA VV  LA+    T GE CGF ++ + H  DDL+L EG++HLLP     +   E + AC+MGLLSQLAAK EVLRV+P   AS+ NAVA+A+ QS+T +ETPL DAG+DGTGEV   VD+GLDETSCFF+DDDGL+++HG L+ G+R + DG++ T  GNY       FP++M+RRK+VQY++MF T +  +  S    +  T  S+Y        F  D           D    +   YS  YS  +W S    C   L+   + + S     S + SS  +S  S+GGF KD   GHGT  AG A G+ S  S    + C G    P CAGGC+S+S+ + +  NG F++D FCP Y+CDG ++  SS CL +D ++ L  HGGVAPGA+I+  DAS+ G    + +AGN LW S++ TGAK+HS+SWG +  CQL E E+ YD+FMYENPE+LLIFAAGN GG +DI   E CT+ SPALGKN+L+VGATS+G SR   TG DG   YEE+G++DYSPEG PWICL P LG PS + +PA  DTV+FFSS GPT DGRIKP++VAPG++V S SSDG++ HSCRLL+  GTS + P+VAGAAALVRQYF+DPSF+ KD+ +RG C E SSSS  C+ F+P+AATVKAM+INSA+ MGGS+ P+  RGFGRVHLEAGMP++G     L V EA I ++ E    V +D  AG ELRATL W+D PT ALS KQLQHDLDL V+AP+G  YTMW +G  D VNV ERV++P ES+ESG +SV VS   LLTD  SYSLVVT AI
Sbjct:   40 FARSQSAYSAASKWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDF-NLSDLSAPLRSTYGEDRSQYDV---------------------------------------------------------TRV-REWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPS---EARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNY------SFPFDMSRRKIVQYIQMFKTPDQGSTSSGQ--NSFTYASDYIRDYLGDAFDDD-----------DSEYNSYDDYSGEYSSDTW-SEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGD-ELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSS-QCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAI 973          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 655 bits (1690), Expect = 2.520e-217
Identity = 417/1030 (40.49%), Postives = 566/1030 (54.95%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP---GVSSVGPLLPSLKLAPSLLDHGFDQGLGDISDVPGTLNLAKP-LQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADP-----EVVHNDGRSTRLSREWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPITSISADGE------ETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEV---VDSGLDETSCFFSDDDGLEIDHGHLYHGLRSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPD-DITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETN-----GLFNIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEA---AIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            F+G    FE A+ W  +RL+    G   P+L CA  G GRE F+ L++ LS  A+  +SH++  G+CF+A+   A  ++++      G+ S  P   +LKLAP L+DH   +      +V G  +  +  L    G ++   +  GL++++ P  LP    G  A+  I +   +L+S +VDLH+ ++W+DP     E + + G + R +R+W +AA+VVH+L+ A   +PG+ C +D + +I A DD +L  GLDHLL  +   A G       E  +ACFMGL+S  A + EVLRV+PRH   + N  A  I QS++ ++TPL DAG+DGTGEV   VD+GLDETSCFF DD G E+DHG+ +  L     GS       Y + +A  F Y   RRKV+QY+E+ +            PD D   FS                                             +T      FL  D                      F +D   GHGTH AG+A G+T    P  T  C G      C GGC+  SE  +   +      L ++D  CP Y CDG+++    CL ++  +TL  +GG+A GAK++ FD  F    LI +  GN +W   L  G KVHS S G +T C  GE E  YD FMYENPENLLIFAAGN G  +      CT+ SPA+GKNVL+VGA+++G +R  +  T+G A             G+                     D V+ FSSYG TTDGRIKPE+VAPG+ V+S +SDG+++HSCRL +Y+GTSM+ P+VAGAAA+VRQYF D +FY  D+ ARG C +G    F C  FSP+AATVKA+LINSAN MG S++PD  RGFGR+HLE GMPL G   L LFVA+A   +I  +    Y  D+D  AGLELRATL WID P  + S  QL +DLDL VV+P+G  Y MWG+   DN NVNERV++ A+ +E+G ++V V    L+TD  SYSLVV  AI
Sbjct:   49 FNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESR------EVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG--AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVR-ARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLL-FSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIY--YPVFEAGDFTYTEERRKVIQYIELVN------------PDSDYVAFS---------------------------------------------TTQGGVYPFLPPD---------------------EFGQDDSAGHGTHTAGSAAGATLN-DPAETLTCSGDETL-GCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD---PCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLI-EVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETER---SVCTMASPAIGKNVLAVGASTSGDTR--VISTEGGA-------------GI---------------------DMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDG----FICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 617 bits (1592), Expect = 5.060e-203
Identity = 401/1032 (38.86%), Postives = 546/1032 (52.91%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP---GVSSVGPLLPSLKLAPSLLDHGFDQGLGDISDVPGTLNLAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADPEVVHNDGRSTRLS--------REWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPITSISADGE------ETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGE---VVDSGLDETSCFFSDDDGLEIDHGHLYHGLRSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEE------LETNGLFNIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEA---AIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            F G    FE A+ W  +RL+        P+L CA  G G EA + L++ LS  A+  +SH++  G+CF AS   A  A+++      G+ +  P    LKLAP L+DH       D ++     +    L    G  + + +  GLS+ + PG L  A   S AK  I +   +L+S +VDLH+ ++W+DP +   DG    LS        R+WR+AA+VVH+L+ A    PG+ C ++RI + HA DD +L  GLDHLL  +   A G       E  +ACFMGL+S LA + EVLRV+PR    + NA A  + QS++ ++TPL DAG+DGTGE   V+DSGLDETSC+F DD G E+DHG                                                                   YY+                        +G +A YSSSYS      +      F  GD+  Y                    + +K GHGTH AG+A G+T    P  T  C  T    +C G C+   +A +       +T+G  ++D  CP Y CD +++  ++CL +D  +TL ++GG+A GAK+A FD  F+GG   S   GN+LW      G K+HS+S G +  C +   +   D FMYEN ENLLIFAAGN GG++      CT+ +P +GKN L++GA+++G +R                +S     G+                     DTV+ FSS+G TTD RIKPE+VAPG+ V+S +SDG++THSCRL +YEGTSM+ P+VAGAAA++RQYF D +FY  D+ ARG C +G    F C  FSP+AATVKA+LINSAN MGGS+ PD  RGFGRVHLEAGMPL G   L+LFVA+A   +I       Y  D+D  AGL+LRATL WID P  A S  QL H+LDL VV+P+G +Y MWG+   DN NVNERV++ A  + +G ++V V   +L+TD  SYSLVV  AI
Sbjct:   44 FKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESH---DEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTL--AARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGM---DGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLL-FSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHG-------------------------------------------------------------------YYF----------------------DELGRAAVYSSSYS------SDETATIFNGGDFSYYP-------------------ERRKAGHGTHTAGSAAGATLN-DPAETITCNVTDTL-SCVGACIDLLDATDDLVSISSQTDGT-DLDRLCPMYGCDNSTD--TTCLSDDVPETLSNNGGMAQGAKLAIFDV-FYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIER---STCTINTPGIGKNSLAIGASTSGDTRV---------------ISTVGATGI---------------------DTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDG----FICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI 903          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 615 bits (1586), Expect = 7.450e-201
Identity = 402/1061 (37.89%), Postives = 565/1061 (53.25%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP---GVSSVGPLLPSLKLAPSLLDHGFDQGLGDISDVPGTLNLAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADPEVVHNDGRSTRLS--------REWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPI------TSISADGEETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEVV---DSGLDETSCFFS-DDDGLEIDHGHLYH--GLR------------SDPDGSI-------DTWKGNYFLV----DANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETNGLFNI-----DTFCPA---YDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGS-ETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEA---AIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            F+G    FE A+ W  +RL+        P+L CA  G GRE F+ L++ LS  A+  +SH++  G+CF AS   A  ++++      G+ S  P   +LKLAP L+DH          D   T  L + L    G  +   +  GLSI + PG L  A     A+  I +   +L+  +VDLH+ ++W+D     N G   +LS        R+W +AA+VVH+L+ A   +PG+ C ++R+ + HA+ D++L  GLDHLL         S   +  E  +ACFMGL+S LA+K EVLRV PR   ++ NA A A  QS+T+++TPL DAG+DGTG+VV   DSG+DE+SCFF+ D+ G  + HGH +   G+             SD D S+       D   G+Y+ +    D   F     RRK++QY+ +  + +       S  D  TT      SLG  Y                                           +L  D +EY                     D + GHGTHVAG+  G+T   +P     C+ T    +C G CV   EA +++     N+     D  CP    YDCD     +  CL +D  +TL ++GG+A GAK+A FDA F+    +S + GN LW        K+HS+S+G +  C LG  +  YD +M  NPENLLIFAAGN G  +      CT+ SPA+ KNVL+VGA+++G +R   T                           P LG           DTV+ FSSYGPT+D RIKPE++APG+ ++S +SDGS ++HSCRL +Y GTSM+TP+VAGA+A++RQYF + +F+  D+ ARG C+E +     C +FSP++AT+KA++INSAN MGGS+ PD SRGFGR+HLEAGMPL G  SL LFV+++   +I       Y  D++ +A L+LR TL W+D P    S  QL HDLDL V++PNG  YTMW +G  D+ NVNERV++ A  +E+G +SV +   +L TD  SYSLVV  AI
Sbjct:   39 FNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHD----KDKETTTGLDR-LSARHGARMRMSNVEGLSIELTPGTL--ASRSVEAEAFISDTLGDLMLASVDLHSTNFWSD----QNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDT----EGSHGDSFTT------SLGQQY------------------------------------------PWLPADRFEY---------------------DYEAGHGTHVAGSVAGATLN-TPAELISCKDTETM-SCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLE--TEKCLSDDVAETLTNNGGMAQGAKLAIFDA-FYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRR---STCTMSSPAIAKNVLAVGASTSGDTRLSAT---------------------------PGLGI----------DTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG----CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 605 bits (1559), Expect = 2.790e-199
Identity = 398/1007 (39.52%), Postives = 528/1007 (52.43%), Query Frame = 0

Query:    1 FSGKHETFEQASDWFSKRLQSHGSGKP-VPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAP--GVSSVGPLLPSLKLAPSLLDHGFDQGLGDISDVPGTLNLAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSAAKDLIRNWRAELVSRTVDLHAISYWADPEVVHNDGRSTRLS----REWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLLPITS--ISADGEETKM--ACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEVV---DSGLDETSCFFSDDDGLEIDHGHLYHGLRSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSS---SEAEELETNGL------FNIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAAIEANHETV---YTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWG-AGEKDNVNVNERVVIPAESIESGKFS 980
            F G  + FE A+ W  + L+S        P+LVC     G +    L+ LLS  A+ P++H+   G+CF  +   A+  ++  +P  G++S+ P   +LK+AP +L+HG    L   S  PG L       T  G  + + +  GL++ + PG LP       A   I     +L+S ++DLH  + W+DP   + +  +T       REW  AAT+VH LA A + +PG+ C +D I M +A +DL+L  GLDHLL   +  I +D E T++  ACFMGL+S L+ + EVLRV+PRH   + NAVA  I QS+TI+E PL DAG+DGTG+V+   D+GLDETSCFF DDDG E+ HGH Y  L     G I               P                        SSS  DD+                                                                                          EGHGTH AG+A G+T +        C GT   P C GGC+ +   S  ++L T          +ID  CP  DCD  ++    CL +D  QTL DHGG+A GAK+AFFD  F  GS  + + GN LW   L  G K+HS S G +  C + E +  YD FMY NPENL++ AAGN+G  +D G   CT+ SPA+GKN L+VGATS+G +R   T  DG        L D                    T+  AD DTV+ FSSYGPT DGRIKPE+VAPG+ ++S  SDGS+ HSCRL +  GTSMA P+VAGA+A+VRQYF D SFYV D  ARG  ++   S   C SFSP++ATVKA+LINSAN MGGS+ PD  RGFGR+HLE GMPL+G  SL LFVA+++  +  E     +  D+D +A L+ RATL WID P  + S  QL HDLDL VV+P+G ++ MW  +GE D+ NVNERV+I AE +ESG ++
Sbjct:   49 FDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPYYGLTSISPFPSALKVAPGVLEHGVS--LDQASQSPGRLT------TTHGHLMRKGNVEGLNMELSPGTLP--AHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEAL-----GEI--------------IP-----------------------SSSSARDDV------------------------------------------------------------------------------------------EGHGTHCAGSALGATIRTPAETPTTCSGT-EVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD--PRCLSDDVGQTLADHGGMAQGAKLAFFDV-FADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED-GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGD-------LID-------------------GTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDD---SGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 591 bits (1524), Expect = 1.820e-192
Identity = 389/1030 (37.77%), Postives = 539/1030 (52.33%), Query Frame = 0

Query:    8 FEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAFASLERLLSTSAIHPISHTDRDGSCFVASLPPADEASVLRAPGVSSVGPLLPSLKLAPSLLDHGFDQGLGDISDVPGTLN--LAKPLQTVFGVSLNQKSTRGLSIRIMPGVLPKAGGGSA-AKDLIRNWRAELVSRTVDLHAISYWADPEVVHND----GRSTRLSREWRRAATVVHDLAAAHKMTPGEACGFDRIRMIHADDDLILFEGLDHLL----PITSISAD-GEETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEVV---DSGLDETSCFFSDDDGLEIDHGHLYHGLRS-DPDGSIDTWKGNYFLVD--ANHFPYNMTRRKVVQYVEMF--DTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETNGLFNIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAA---IEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVT-----------VSVGDLLTDFHSYSLVVTAAI 1003
            F  AS W +   Q    G+  PYL C   G GR A+  LE  L   A+ P+S T   G+CF+ +  PA+ A +      ++ G    +LK+AP LLDHG   G     D   T +   A  L T  G S+   S  GL + + PG LP   GG A A + I+     L S ++DLHAI+ W+D  +   D           REW RAAT+V++ + + + TP + CG++++ + HA +D++L  GLDHLL     ++  S +   E +MAC+MGL+S LA++ EVL+V   +     NA A A  QS+++  TPL DAG+DG+GEV+   D+G++ET+CF    DG E++HG+ +  + S +P  +  +   + F  +     F Y+  RRKV+QY+++   DTG+ +   SS +P                                                            F+  D                      F  D   GHGTH A T  GST   SP     C        CAGGC++ S     E +   +I+  CPA+ C G       CL +DT  TL +HGG+A GAKIA  D  F+G     D AGN LW +    G K+HS+S+G++  CQL  ++  YD +MYEN E+LL+F+AGN+G   D   + CT+ SP + KNVL+VG+TS+G +R   T  DGR   +   LS                            D VS FS YG T DGRIKPE+VAPG+ ++S S +G ++ SC L++Y GTS +T V +GAAA+VRQYFRD SFY  D+E+RG C++G    F C+SFSP A TVKAMLINSAN MGG   PD  RGFGR+HLEAGMPL G   + LFVA+++   + ++ +  +  ++D  AG+ELRATL W D    +LS  QL +DLDL V AP+G  Y MW     D  NVNERV++PAE +   +FS T            +VGD +    S+SLVVT AI
Sbjct:   48 FAFASAWQAGDRQ----GRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWEDFTTFGAFPAALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGS---SSGMP------------------------------------------------------------FVSAD---------------------SFVSDDDLGHGTHAATTVAGSTIS-SPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSG-----DQCLPDDTADTLSEHGGMAQGAKIAAIDV-FYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDR--DTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTI--------------------------DVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQG----FTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVV--AQFSATDEWEVRVRGRDFTVGDTV----SFSLVVTGAI 943          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 541 bits (1393), Expect = 1.730e-171
Identity = 396/1117 (35.45%), Postives = 561/1117 (50.22%), Query Frame = 0

Query:    8 FEQASDWFSKRLQSHGSGKPVPYLVCAGKGSGREAF-ASLERLLSTSAI--HPISHTDRDGSCFVASLPPADEASVLRAPGVSSVGPLLPS-LKLAPSLLDHGFDQGLGDISDVPGTLN------LAKPLQT----------------------------------VFGVSLNQKSTRGLSIRIMPGVLP------KAGGG--SAAKDLIRNWRAELVSRTVDLHAISYWA--------DPEVVHNDGRSTR------LSREWRRAATVVHDLAAAHK-MTPGEACGFDRIRMIHADDDLILFEGLDHLLPIT-----SISADGEETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEV---VDSGLDETSCFFSDDDGLE-IDHGHLYH--GLRSDPDGS-IDTWKGNYFLV---DANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACG------STSQGSPFVTQDCRGTGAFPACAGGCVSSSEA---EELETNGLF----------NIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSN-TSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAAIEANHE---TVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESI---------------ESGKFSVTVSVGDLL-TDFHSYSLVVTAAI 1003
            F+ A +W   RL      +P PYL CA   S R    A+L  +L   AI    +  +   G C VA+  P    S+ +   V S     PS LKLAP LLDH  D   G   D  G L       L +P                                        +        GL++ + PGVL        +GGG  +++ +L   WR EL+S+++D+H  S+W+        D       G S+       L+REW RAA V+  L       T G+ C +D + ++H DDDL+  +G+DHLLP       +   + +E K+ACFM LLS LA++ EVLRV+P     + NA A    QS++++E+P+ DAG+DGTGEV   VD+GLD  SCFF D+DG E + HG+ +   G+ +   GS   +W  N  +        F ++++RRK +       + ++ N+       D+                  WSG         +N   +A+ S++Y   SW ++         G+ + ++S++              F +D   GHGTH AG+A G      S SQ   F T+ C   G   +C GGC+   +    ++L T   +          ++D  CP  DCD   E    CLG+D  +TL  HGGVA GA++A FD       +  D AGN +W  ++  G+K+ S+SWG+  +C    ++  +D FMY N + LL+FAAGN G      +  CT+ +PAL KNVL+VGA+++G  R   T  D                           G P++  S+  D D +++FSS+GPT D RIKPE+VAPG+ ++S +  G   ++CRL  Y GTSM+TP+VAGAAALVRQYF++ +FY  D+E RG C+     SF C  F+P+AATVKAMLINSAN MGGS  PD  RGFGRVHLEAGMPL G     LFVA++A+ +  E   T Y+  +D +AG++LRATL WID P    S+ QL HDLDL V +P G+ Y+MW  G  D++N NERV++PA +I                 G ++V V    L   +  SYSLVVT AI
Sbjct:   53 FKAAVEWHEARLDD---ARPAPYLACASPDSDRGGSGATLSHVLGAGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFPSALKLAPGLLDHPHDDRDGGGGD--GRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVEASSFGSWFTSWDFNVQIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRA----------------WSG---------ENPACTANNSTNYG-ESWATSA--------GEQYPWISAAE-------------FAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDF-TEVCE-EGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCDDLPEEY--CLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD----DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDED---------------------------GDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGDGNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCD-----SFGCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAI 1077          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 476 bits (1225), Expect = 1.390e-151
Identity = 313/779 (40.18%), Postives = 426/779 (54.69%), Query Frame = 0

Query:  245 GLDHLLPITSISADGE------ETKMACFMGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGE---VVDSGLDETSCFFSDDDGLEIDHGHLYHGL-RSDPDGSIDTWKGNYFLVDANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGST--YFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEE-----LETNGLFNIDTFCPAYDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGSETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEA---AIEANHETVYTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            GLDHLL  +   A G       E  +ACFMGL+S LA + EVLRV+PR    + NA A  I QS++ ++TPL DAG+DGTGE   V+DSGLDETSC+F DD G E+DHG+ +  L ++    S+ +      + +   F Y   RRK++QY+++ +            PD  + +S +Y S GS   YF                                     P  + F E D                          KK+     V  +A G+T   +P  T  C  T    +C G C+   +  +         G  ++D  CP Y CD +++  ++CL +D  +TL ++GG+A GAK+A FD  F+G    S   GN+LW      G K+HS+S G +  C +   +   D FMYEN E+LLIFAAGN GG +      CT+ +P +GKN L++GA+++G +R                +S     G+                     +TV+ FSS+G TTD RIKPE+VAPG+ V+S +SDG++THSCRL +YEGTSM+ P+VAGAAA++RQYF D +FY+ D+ ARG C +     F C  FSP+AATVKA+LINSAN MGGS+ PD  RGFGRVHLEAGMPL G  +L+LFVA+A   +I       Y  D+D  AGL+LRATL WID P+ A S  QL H+LDL VV+P+G +Y MWG+   DN NVNERV++ A  + +G ++V V    L+TD  SYSLVV  AI
Sbjct:    5 GLDHLL-FSGRGAGGSHEEESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDELAQAAVYSSLYSSDETATIFNGGDFSYYPERRKIIQYIDLVN------------PD--SPYSSFYTSQGSYIGYF------------------------------------LPPDQ-FGEDD-------------------EARVALRKKDPSQARVGRSAAGATLN-NPAETITCNVTDTL-SCVGACIDLLDVTDDLVSISHQTGGTDLDRLCPMYGCDNSTD--TTCLSDDVPETLSNNGGMAQGAKLAIFDV-FYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEER---STCTINTPGIGKNSLAIGASTSGDTRV---------------ISTVGATGI---------------------NTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDV----FICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAI 664          

BLAST of mRNA_F-serratus_M_contig872.20087.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 476 bits (1226), Expect = 2.340e-151
Identity = 304/778 (39.07%), Postives = 421/778 (54.11%), Query Frame = 0

Query:  268 MGLLSQLAAKQEVLRVTPRHGASISNAVASAITQSSTISETPLHDAGIDGTGEVV---DSGLDETSCFFS-DDDGLEIDHGHLYHGLRSDPD----------------------GSIDTWKGNYFLV----DANHFPYNMTRRKVVQYVEMFDTGENNNVGSSSIPDDITTFSEYYYSLGSTYFSSDWSGSSSTTSSGDQNMGASASYSSSYSYSSWPSTTPACRDFLEGDWWEYLSSSTWSSMFTSSFSSGGFHKDKKEGHGTHVAGTACGSTSQGSPFVTQDCRGTGAFPACAGGCVSSSEAEELETNG-----LFNIDTFCPA---YDCDGTSENSSSCLGEDTLQTLVDHGGVAPGAKIAFFDASFHGGSLISDWAGNSLWNSSLATGAKVHSSSWGMETFCQLGEMEFNYDTFMYENPENLLIFAAGNSGGLKDIGFEPCTVISPALGKNVLSVGATSAGASRAPLTGTDGRARYEEYGLSDYSPEGVPWICLYPFLGQPSNTSKPADKDTVSFFSSYGPTTDGRIKPELVAPGERVFSVSSDGS-ETHSCRLLSYEGTSMATPVVAGAAALVRQYFRDPSFYVKDLEARGLCEEGSSSSFPCNSFSPTAATVKAMLINSANAMGGSTRPDNSRGFGRVHLEAGMPLNGNNSLILFVAEAAIEANHETV---YTVDIDDSAGLELRATLCWIDTPTIALSTKQLQHDLDLFVVAPNGVSYTMWGAGEKDNVNVNERVVIPAESIESGKFSVTVSVGDLLTDFHSYSLVVTAAI 1003
            MGL+S LA+K EVLRV PR   ++ NA A A  QS+T+++TPL DAG+DGTG+VV   DSG+DE+SCFF+ D+ G  + HGH +     +P                        + D   G+Y+ +    D   F     RRK++QY+ +  + +       S  D  TT      SLG  Y                                           +L  D +EY                     D + GHGTHVAG+  G+T   +P     C  T    +C G CV   EA +++        + ++D  CP    YDCDG    +  CL +D  +TL ++GG+A GAK+A FDA F+    +S + G  LW        K+HS+S+G +  C LG  +  YD +M  NPENLLIFAAGN G L+      CT+ SPA+ KNVL+VGA+++G +R  L+ T G               G+                     DTV+ FSSYGPT+D RIKPE++APG+ ++S +SDGS +THSCRL +Y GTSM+TP+VAGA+A++RQYF + +F+  D+ ARG C+E +     C +FSP++AT+KA++INSAN MG S+ P+ SRGFGR+HLEAGMPL G  SL LFV+++   +  E     Y  D++ +AGL+LR TL W+D P    S  QL HDLDL V++PNG  YTMW +G  D+ NVNERV++ A+ +E+G +SV +   +L TD  SYSLVV  AI
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDT----EGSHGDSFTT------SLGQQY------------------------------------------PWLSADHFEY---------------------DDEAGHGTHVAGSVAGATLN-TPAELISCNDTETM-SCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLE--TDKCLSDDVAETLTNNGGMAQGAKLAIFDA-FYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRR---STCTIGSPAIAKNVLAVGASTSGDTR--LSATPG--------------SGI---------------------DTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG----CEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 656