mRNA_F-serratus_M_contig999.21263.1 (mRNA) Fucus serratus male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-serratus_M_contig999.21263.1
Unique NamemRNA_F-serratus_M_contig999.21263.1
TypemRNA
OrganismFucus serratus male (Fucus serratus male (Toothed wrack or serrated wrack))
Homology
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5KP87_9PHAE (Endo/exonuclease/phosphatase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KP87_9PHAE)

HSP 1 Score: 145 bits (367), Expect = 1.090e-40
Identity = 85/171 (49.71%), Postives = 109/171 (63.74%), Query Frame = 1
Query:    7 CDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVR----NSITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFA 489
            CD+IGLQETRR+GQ A +  GY++  SG R G  D K+G  GVG A++    +S+    R  E IS RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR  G     D+KI+GAYGRD +N+NGE LL  A
Sbjct:   12 CDVIGLQETRRAGQGAITHDGYVIIWSGARAGTKD-KRGVHGVGIAIKETMWDSVGEGGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETARNTAGGDVNAKDSFWTALDAEIREVHSRDHLVVLMDANARTGRRRDGCC---DAKIMGAYGRDIMNNNGERLLGLA 177          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5L660_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L660_9PHAE)

HSP 1 Score: 148 bits (374), Expect = 2.810e-40
Identity = 85/174 (48.85%), Postives = 108/174 (62.07%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQNA------NKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E I  RL+KV L + GR   +TFVV YAPT+          N K +FWT+LD V+ EV  H+ L VLM+ANARTGRR  G     D+KI+G YGRD +N+NGE LL  A+N
Sbjct:  181 GCDVIGLQETRREGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKETMWESVGEEGRKVECIGPRLMKVRLRI-GRTCGITFVVRYAPTETVRTTAGGDINAKDSFWTALDAVIREVHSHDHLVVLMDANARTGRRRDGCC---DAKIMGVYGRDIMNNNGERLLGLASN 349          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5JMM5_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMM5_9PHAE)

HSP 1 Score: 142 bits (358), Expect = 2.270e-39
Identity = 83/173 (47.98%), Postives = 109/173 (63.01%), Query Frame = 1
Query:    7 CDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            CD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E IS RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR   +VG  D K++GAYGRD + +NGE LL  A++
Sbjct:   12 CDVIGLQETRREGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGRAIKEAMWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTTAGGDVNAKDSFWTALDAAIREVHSRDHLVVLMDANARTGRR---RVGCCDGKMMGAYGRDIMTNNGERLLGLASD 179          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5KBZ6_9PHAE (Endo/exonuclease/phosphatase domain-containing protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KBZ6_9PHAE)

HSP 1 Score: 145 bits (366), Expect = 3.260e-39
Identity = 84/174 (48.28%), Postives = 109/174 (62.64%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E IS RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR  G     D+K+LGAYGRD +N+NGE L   A++
Sbjct:   11 GCDVIGLQETRREGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKEAMWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTTAGGDVNAKDSFWTALDAAIREVHSRDHLVVLMDANARTGRRRGGCC---DAKVLGAYGRDIMNNNGERLFGLASD 179          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5KTK0_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KTK0_9PHAE)

HSP 1 Score: 147 bits (371), Expect = 5.300e-39
Identity = 85/174 (48.85%), Postives = 110/174 (63.22%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQNANK------KHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E IS RL+KV L++ GR   VTFVV YAPT+    N       K +FWT+LD V+ EV  H+ L V M+ANARTGR+  G     D+KI+GAYG DT+N+NGE LL  A++
Sbjct:   11 GCDVIGLQETRREGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKEAMWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTNAGGDVHAKESFWTALDAVMREVHSHDHLVVPMDANARTGRKRDGCC---DAKIMGAYGHDTMNNNGERLLGLASD 179          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5K3Q1_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K3Q1_9PHAE)

HSP 1 Score: 148 bits (374), Expect = 1.040e-38
Identity = 85/174 (48.85%), Postives = 112/174 (64.37%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR+GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E IS RL+KV L++ GR   VTF+V YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR KG     D+KI+GAYGRD +N+NGE LL  A++
Sbjct:  208 GCDVIGLQETRRAGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKEAMWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFLVGYAPTETVRTTEGCDVNAKDSFWTALDAAIREVHSRDHLVVLMDANARTGRRRKGCC---DAKIMGAYGRDIMNNNGERLLGLASD 376          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5K259_9PHAE (Endo/exonuclease/phosphatase domain-containing protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K259_9PHAE)

HSP 1 Score: 143 bits (361), Expect = 2.460e-38
Identity = 84/173 (48.55%), Postives = 109/173 (63.01%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFAN 492
            GCD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E I+ RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR  G     D+KI+GAYGRD +N+NGE LL  A+
Sbjct:   11 GCDVIGLQETRREGQGSLAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKEAMWESVGEKGRTVECINPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTTAGGDVNAKDSFWTALDGEIREVHSRDHLVVLMDANARTGRRRDGCC---DAKIMGAYGRDIMNNNGERLLGLAS 178          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5KZE6_9PHAE (Endo/exonuclease/phosphatase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KZE6_9PHAE)

HSP 1 Score: 142 bits (357), Expect = 2.750e-38
Identity = 84/174 (48.28%), Postives = 109/174 (62.64%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRNSITRAA----RPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQNA------NKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR GQ + +  GY++  SG R G  D K+G  GVG A++ +I  +     R  E IS RL+KV L++ GR   VTFVV YAPT+   +      N K +FWT+LD  + EV   + L VLM ANARTGRR  G     D+KI+GA GRD +N+NGE LL  A++
Sbjct:   42 GCDVIGLQETRREGQGSIAHDGYVIIWSGARAGTKD-KRGGHGVGIAIKEAIWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRSTAGGDVNAKDSFWTALDAAIREVHSRDHLVVLMGANARTGRRRDGCC---DAKIMGANGRDIMNNNGERLLELASD 210          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5JMB4_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JMB4_9PHAE)

HSP 1 Score: 147 bits (371), Expect = 3.990e-38
Identity = 85/174 (48.85%), Postives = 111/174 (63.79%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR+GQ + +  GY++  SG R G  D K+G  GVG A++     S+    R  E IS RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR  G     D+KI+GAYGRD +N+NGE LL  A++
Sbjct:  119 GCDVIGLQETRRAGQGSIAHDGYVIIWSGARAGTKD-KRGVHGVGIAIKEAMWESVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTTAGGDVNAKDSFWTALDAAIREVHSRDHLVVLMDANARTGRRRDGCC---DAKIMGAYGRDIMNNNGERLLGLASD 287          
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Match: A0A6H5KQ63_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KQ63_9PHAE)

HSP 1 Score: 143 bits (360), Expect = 7.770e-38
Identity = 85/174 (48.85%), Postives = 108/174 (62.07%), Query Frame = 1
Query:    4 GCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVRN----SITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQ------NANKKHAFWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRDTLNDNGELLLSFANN 495
            GCD+IGLQETRR GQ + +  GY+   SG R G   GK G  GVG A++     S+    R  E IS RL+KV L++ GR   VTFVV YAPT+        + N K +FWT+LD  + EV   + L VLM+ANARTGRR  G     D+KI+GAYGRD +N+NGE LL  A++
Sbjct:   11 GCDVIGLQETRREGQGSIAHDGYVFIWSGARAGTK-GKWGVHGVGIAIKEAMWQSVGEEGRTVECISPRLMKVRLQI-GRTCGVTFVVGYAPTETVRTTAGGDVNAKDSFWTALDAAIREVHSRDHLVVLMDANARTGRRRDGCC---DAKIMGAYGRDIMNNNGERLLGLASD 179          
The following BLAST results are available for this feature:
BLAST of mRNA_F-serratus_M_contig999.21263.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KP87_9PHAE1.090e-4049.71Endo/exonuclease/phosphatase domain-containing pro... [more]
A0A6H5L660_9PHAE2.810e-4048.85Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JMM5_9PHAE2.270e-3947.98Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
A0A6H5KBZ6_9PHAE3.260e-3948.28Endo/exonuclease/phosphatase domain-containing pro... [more]
A0A6H5KTK0_9PHAE5.300e-3948.85Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
A0A6H5K3Q1_9PHAE1.040e-3848.85Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
A0A6H5K259_9PHAE2.460e-3848.55Endo/exonuclease/phosphatase domain-containing pro... [more]
A0A6H5KZE6_9PHAE2.750e-3848.28Endo/exonuclease/phosphatase domain-containing pro... [more]
A0A6H5JMB4_9PHAE3.990e-3848.85Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
A0A6H5KQ63_9PHAE7.770e-3848.85Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-serratus_M_contig999contigF-serratus_M_contig999:160584..161105 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Fucus serratus MALE vs UniRef902022-09-19
OGS1.0 of Fucus serratus male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score85.1
Seed ortholog evalue3.5e-14
Seed eggNOG ortholog7668.SPU_020204-tr
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Ribonuclease H protein
EggNOG OGs39TZY@33154,3BHR3@33208,3CUIP@33213,KOG1075@1,KOG1075@2759
COG Functional cat.S
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
Exons1
Model size522
Cds size522
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622932798.0831783-CDS-F-serratus_M_contig999:160583..1611051622932798.0831783-CDS-F-serratus_M_contig999:160583..161105Fucus serratus maleCDSF-serratus_M_contig999 160584..161105 -
1690964479.7805161-CDS-F-serratus_M_contig999:160583..1611051690964479.7805161-CDS-F-serratus_M_contig999:160583..161105Fucus serratus maleCDSF-serratus_M_contig999 160584..161105 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-serratus_M_contig999.21263.1prot_F-serratus_M_contig999.21263.1Fucus serratus malepolypeptideF-serratus_M_contig999 160584..161105 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-serratus_M_contig999.21263.1

>prot_F-serratus_M_contig999.21263.1 ID=prot_F-serratus_M_contig999.21263.1|Name=mRNA_F-serratus_M_contig999.21263.1|organism=Fucus serratus male|type=polypeptide|length=174bp
LGCDIIGLQETRRSGQSAFSQAGYLVYCSGERGGENDGKKGQGGVGCAVR
NSITRAARPPEFISDRLLKVTLELRGRAKAVTFVVAYAPTKPQNANKKHA
FWTSLDIVVEEVPKHEQLFVLMEANARTGRREKGQVGSKDSKILGAYGRD
TLNDNGELLLSFANNCCGRARLS*
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mRNA from alignment at F-serratus_M_contig999:160584..161105-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-serratus_M_contig999.21263.1 ID=mRNA_F-serratus_M_contig999.21263.1|Name=mRNA_F-serratus_M_contig999.21263.1|organism=Fucus serratus male|type=mRNA|length=522bp|location=Sequence derived from alignment at F-serratus_M_contig999:160584..161105- (Fucus serratus male)
CTGGGGTGCGACATCATCGGTTTGCAGGAAACTCGCCGTAGCGGACAGTC AGCCTTCAGTCAGGCTGGCTACCTGGTCTACTGCAGTGGTGAGCGCGGTG GCGAAAATGATGGGAAGAAAGGGCAAGGTGGAGTAGGGTGTGCTGTAAGA AACTCCATCACACGTGCTGCACGCCCACCGGAGTTTATCAGTGATCGCTT GTTAAAGGTTACTCTTGAACTACGGGGCCGAGCCAAGGCCGTTACGTTTG TCGTGGCATATGCCCCAACTAAACCACAGAATGCAAATAAAAAACATGCA TTCTGGACGAGCCTAGACATAGTTGTGGAAGAAGTACCTAAACATGAACA GCTGTTCGTGTTGATGGAGGCCAACGCCCGCACGGGAAGGAGGGAAAAGG GACAGGTGGGGAGCAAGGATAGCAAAATTCTCGGTGCCTACGGCCGAGAT ACCCTCAACGACAACGGAGAACTACTGCTGTCCTTCGCTAACAACTGTTG CGGCAGAGCAAGGCTGTCATAG
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Coding sequence (CDS) from alignment at F-serratus_M_contig999:160584..161105-

>mRNA_F-serratus_M_contig999.21263.1 ID=mRNA_F-serratus_M_contig999.21263.1|Name=mRNA_F-serratus_M_contig999.21263.1|organism=Fucus serratus male|type=CDS|length=1044bp|location=Sequence derived from alignment at F-serratus_M_contig999:160584..161105- (Fucus serratus male)
CTGGGGTGCGACATCATCGGTTTGCAGGAAACTCGCCGTAGCGGACAGTC
AGCCTTCAGTCAGGCTGGCTACCTGGTCTACTGCAGTGGTGAGCGCGGTG
GCGAAAATGATGGGAAGAAAGGGCAAGGTGGAGTAGGGTGTGCTGTAAGA
AACTCCATCACACGTGCTGCACGCCCACCGGAGTTTATCAGTGATCGCTT
GTTAAAGGTTACTCTTGAACTACGGGGCCGAGCCAAGGCCGTTACGTTTG
TCGTGGCATATGCCCCAACTAAACCACAGAATGCAAATAAAAAACATGCA
TTCTGGACGAGCCTAGACATAGTTGTGGAAGAAGTACCTAAACATGAACA
GCTGTTCGTGTTGATGGAGGCCAACGCCCGCACGGGAAGGAGGGAAAAGG
GACAGGTGGGGAGCAAGGATAGCAAAATTCTCGGTGCCTACGGCCGAGAT
ACCCTCAACGACAACGGAGAACTACTGCTGTCCTTCGCTAACAACTGTTG
CGGCAGAGCAAGGCTGTCATAGCTGGGGTGCGACATCATCGGTTTGCAGG
AAACTCGCCGTAGCGGACAGTCAGCCTTCAGTCAGGCTGGCTACCTGGTC
TACTGCAGTGGTGAGCGCGGTGGCGAAAATGATGGGAAGAAAGGGCAAGG
TGGAGTAGGGTGTGCTGTAAGAAACTCCATCACACGTGCTGCACGCCCAC
CGGAGTTTATCAGTGATCGCTTGTTAAAGGTTACTCTTGAACTACGGGGC
CGAGCCAAGGCCGTTACGTTTGTCGTGGCATATGCCCCAACTAAACCACA
GAATGCAAATAAAAAACATGCATTCTGGACGAGCCTAGACATAGTTGTGG
AAGAAGTACCTAAACATGAACAGCTGTTCGTGTTGATGGAGGCCAACGCC
CGCACGGGAAGGAGGGAAAAGGGACAGGTGGGGAGCAAGGATAGCAAAAT
TCTCGGTGCCTACGGCCGAGATACCCTCAACGACAACGGAGAACTACTGC
TGTCCTTCGCTAACAACTGTTGCGGCAGAGCAAGGCTGTCATAG
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