mRNA_F-serratus_M_contig1528.3624.1 (mRNA) Fucus serratus male

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: D7FNH7_ECTSI (Tetracycline-efflux transporter n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNH7_ECTSI)

HSP 1 Score: 463 bits (1191), Expect = 2.280e-148
Identity = 261/476 (54.83%), Postives = 315/476 (66.18%), Query Frame = 2

Query:  134 RRLTLCLFLSAITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGLGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALLSLFVLGWEETAPKRVAAS---------EEDDAESDA-------------------------------ETTGSVDG----SDCEQQRGTEGKIRP---KISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFSAGCTVRFEQAPFFLAAIFSFVSLTITWWPLREMDKRQRTL 1420
            R+LT C+ L  I  S+++QVYPKIILL   GDD +AS YRGWLA  VSV QL+FVP +   SDVVGR+AV+  A VLH  +   L+    SL WAT+  L T +CIV +P+SQAIMID++   GKGATHGLGIA+GA AL  S GDIIGGSL+E +R  ACLV+   S A+LLSL + GW ETAP  V            EE +   DA                               E T SVD      D +Q  G  G+ R    K  NP+S+  VFLESR LL+IA SYFLF+LSLNVFA GYNY+D+RF W+P EISYFFAT+N LMAL  G  IRFIVP+ L+EE GALFG  +Q  + +  GLC+RGWML+PAL FGALQNITEPCLQAIMATFV  + QGSLQGAV SLR++GEGVAAP+F+  FSAG +V FE+APFFLA   S VSL + WWPLR++DK +  +
Sbjct:   74 RKLTACILLYTIGASMMTQVYPKIILLSCGGDDGEASKYRGWLAAGVSVIQLIFVPAISGWSDVVGRKAVVCFAQVLHAGSVLALAAMADSLAWATVCRLATSVCIVILPVSQAIMIDLSPDRGKGATHGLGIAFGASALGFSAGDIIGGSLTEHHRGAACLVSAAFSAASLLSLALFGWRETAPPLVGEGWQRWLWWRVEEQERIDDAVVGMNDDDDVGANRGICGRDVAAVVQGGDAVEATASVDSFVWDDDGKQGAGGRGRARRSGRKQLNPLSVLKVFLESRALLRIACSYFLFVLSLNVFATGYNYVDYRFGWTPSEISYFFATYNILMALAGGWVIRFIVPKRLTEEKGALFGISVQVFSVMVSGLCFRGWMLYPALVFGALQNITEPCLQAIMATFVGPELQGSLQGAVMSLRVVGEGVAAPVFTQVFSAGASVGFEEAPFFLATAISLVSLAVAWWPLRKLDKTKNIV 549          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: D7FNH6_ECTSI (Major facilitator transporter n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FNH6_ECTSI)

HSP 1 Score: 338 bits (866), Expect = 7.600e-102
Identity = 198/448 (44.20%), Postives = 273/448 (60.94%), Query Frame = 2

Query:   89 AVNVPQQLIAAAVVRRRLTLCLFLSAITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDI----AAGGGKGATHGLGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFSAGCTVRFEQA--------PFFLAAIFSFVSLTITWWPLRE 1396
            A +VP+ L     VRRRL++CLFL  +  +I+SQVYPK++L HF+ D+ +AS + GWL     + QL+ +P LC  SDV+GRRA++AGA  L   ++F L   PSS+   ++  +V+ +    +P+SQA++ID+    AA GG   THGLG+   A  LA+SVG ++GGSL+E +   AC ++  +S+  +LSL   GWEETAP   A      A ++A   G   G      RG+  +I     NP S+  VFLES LL+Q+A+    F LSLNVF+V YNY DFR+HWSPL+IS FF+TF  L+AL SGL IRF+VP+ LS   G L G  +Q  +   +GL  +GWM +  L    LQ ITEPC+Q +MA  V  DRQGSLQGAV  LR + +G+A  ++   FS G + R E A        P F A+     +LTI W+ LR+
Sbjct:   36 ATSVPRGLTKGDRVRRRLSVCLFLHIVGVAIISQVYPKVVLEHFDHDNGEASRFTGWLTLVSCIVQLIAIPTLCGASDVIGRRAILAGALALTAVSSFSLGLAPSSIAVVSVCQVVSGVANAILPISQAVVIDLSHWGAAAGGGEVTHGLGLIGAAFGLAVSVGPVLGGSLTEDHAGTACWLSASMSVLGVLSLKFFGWEETAP--AATQGAGGATANASAGG---GWKKLCARGSSSRI----VNPFSVLKVFLESSLLMQLAVVLACFSLSLNVFSVYYNYFDFRYHWSPLDISLFFSTFGILLALTSGLLIRFLVPKRLSVGRGVLLGLALQSSSTTLFGLASQGWMCYVVLVATVLQYITEPCIQGVMANHVGADRQGSLQGAVQCLRNVSQGLAGVLYGQIFSLGVSTRVENALGFVLPGLPLFCASGCGATALTIAWYALRK 474          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A6H5L7V6_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L7V6_9PHAE)

HSP 1 Score: 203 bits (516), Expect = 6.710e-55
Identity = 111/194 (57.22%), Postives = 138/194 (71.13%), Query Frame = 2

Query:  116 AAAVVRRRLTLCLFLSAITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGLGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALLSLFVLGWEETAPKRV 697
            AAA V R+LT C+ L  I  S+++QVYPKIILL   GDD +AS YRGWLA  VSV QL+FVP +   SDVVGR+ V+  A VLH ++   L+    SL+WAT+  L T +CIV +P+SQAIMID++   GKGATHGLGIA+GA AL  S GDIIGGSL+E +R  ACLV+   + A+LLSL + GW ETAP  V
Sbjct:   28 AAAAVHRKLTACILLYTIGASMMTQVYPKIILLSSGGDDGEASKYRGWLAAGVSVIQLLFVPAISGSSDVVGRKVVVCFAQVLHASSVLALAAMADSLVWATVCRLATSVCIVILPVSQAIMIDLSPDRGKGATHGLGIAFGACALGFSAGDIIGGSLAEHHRGAACLVSAAFATASLLSLMLFGWRETAPPLV 221          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A835YXD6_9STRA (Major facilitator superfamily domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YXD6_9STRA)

HSP 1 Score: 155 bits (393), Expect = 1.420e-35
Identity = 133/492 (27.03%), Postives = 208/492 (42.28%), Query Frame = 2

Query:  176 SILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGLGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSM-----------AALLSLFVL---GWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEG-KIRPKISNPVSIFTVFLESRLLLQI--------------------------------------------ALSYFLFILSLNVFAVGYNYLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFSAGCT---------VRFEQAPFFLAAIFSFVSLT--ITWWPLREMDKRQRTLDDGASAD 1441
            +I +QVYPK++L ++ GDD K +++ GW++   S+ Q V  P L +  DV GRR ++  A        F+   +P+++  A  + ++T +C V IP+SQAI+ D+    G  A HG G+  G   L L +G ++GG L+  +R  AC  A G ++            ALL+L V    GWEET     A                 D S    +R     +   ++ NPV+I    L  R   ++                                            A  YF    ++  F   Y  LD+RF W P++I  F A F   +  V  L IR +VPR +++      G  IQ      +G+  +GW L+      AL +          A      R GSLQGA+ SLRI+ +G+A P++   F+ G +          R     F  AA+ S   L   ++  P+  + ++  + DD A A+
Sbjct:  202 AIQAQVYPKVVLSYYGGDDGKTASFLGWVSGGSSMLQFVLSPSLTASCDVRGRRGLLLAAAGAQALICFIWGFSPNNIGLAAAVAVITGMCGVVIPVSQAIISDVTRHDGAAAAHGFGVIAGTFGLGLCIGPLLGGWLTNVHRPAACFTAAGAALLAHCWWRWWAGTALLALLVATVWGWEETYVPDAA-----------------DASRAALRRSMSSTRYHWQVVNPVAIIGTVLHKRRTRRLPSAAQAARAQTVVSGPLSAAGCTRXXXXXXXXXXXXXXXXXXXXACVYFCACTAIGSFNSWYTLLDYRFGWGPVQIGAFCAAFGLGITAVQSLVIRAVVPRLMTQARAVPVGMAIQALGMGLFGIANKGWELYAITPISALSS----------AAATDASRLGSLQGALFSLRILSQGLAGPLYGALFNLGISDALRDALGGTRLPGLAFLAAALASLAGLAFALSVPPVAMLSEKALSGDDAALAE 666          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: UPI001BE1A699 (TCR/Tet family MFS transporter n=1 Tax=Dyadobacter sp. CECT 9275 TaxID=2822344 RepID=UPI001BE1A699)

HSP 1 Score: 102 bits (255), Expect = 1.590e-19
Identity = 107/413 (25.91%), Postives = 177/413 (42.86%), Query Frame = 2

Query:  152 LFLSAITTSILSQVYPKIILLHFEGDD-RKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGL-GIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTE-GKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYN-YLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFS----AGCTVRFEQAPFFLAAIFSFVS 1366
            L +  I   I+  V PK+I     GD   +AS   GWL  A S TQ +  P++  +SD  GRR V+  +    G     L+  P+ L W  +  ++  L   +     A + DI+    + A  GL G A+G   L   +G ++GG L +       + A GLS+   L  + +  E   P                          E +R  E G+     +NP+          ++  +  S  L  ++L+     ++ Y+  +F W+  +I Y       L A + G  IR I+P+ L ++ G   G  +       Y     GW ++  +    L +I  P LQ I++T VA++ QG LQGA+TSL+ +   +  P+ ++ F+    AG  + F  AP  +AA  + +S
Sbjct:   16 LLIDIIGLGIIIPVMPKLIA-ELTGDSISEASRDGGWLLFAYSFTQFICAPIIGGLSDRFGRRPVLLASLFGFGIDYIFLAFAPT-LAWLYVGRVIAGLMGASFTTGAAYIADISTPEKRAANFGLLGAAFG---LGFIIGPVMGGLLGQYGSRVPFMAAAGLSLLNFLYGYFILPESLLP--------------------------ENRRKFEWGR-----ANPIGSLQRLKNYPVIYGLIASLTLLYIALHAVQSNWSFYVIEKFGWNEAQIGYSLGAVGLLSAFIQGYLIRLILPK-LGQKRGVYLGLLLYAVGFGLYAFASEGWTMYVFMIPYILGSIAGPSLQGIISTQVARNEQGELQGALTSLQSLTSIIGPPLMTNLFAVFTKAGTQLYFPGAPMLVAAFLTLIS 391          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A3D1ELS5_9GAMM (Tetracycline resistance MFS efflux pump n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D1ELS5_9GAMM)

HSP 1 Score: 98.6 bits (244), Expect = 4.140e-18
Identity = 112/438 (25.57%), Postives = 181/438 (41.32%), Query Frame = 2

Query:  143 TLCLFLSAITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLV-TCLCIVNIPLSQAIMIDIAAGGGKGATHGL-GIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMA-ALLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLDF-RFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGAL-FGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLR----IIGEGVAAPIFSHTFSAGCTVRFEQAPFFLAAIFSFVSLTITWWPLREMDKRQRTLDDG 1429
            T+ LFL  +   ++  ++P +++    GD   +S   G+L T  +V QL+F P+L S+SD  GRR V+  +         +L    S  +W   L  V T +       + A++ D++    +    GL GIA+G   L    G  +GG L           A GL++   +  LF L  +ET PK                          +QR T      K +NPV           L  +    FL+ +  +VF   +N+    RF WSPL++    A    +   V G  +R+++P+  +E       G  I    GVA         L+  +A  +L  +T P +Q+IM   + ++ QG LQG + S+     I+G       F++  S    +    A F  AA  + +SL I    +R+  +  R L  G
Sbjct:   11 TITLFLDTVGFGLIIPIFPTLLVEITGGDMASSSAMSGYLTTVFAVAQLLFSPLLGSLSDRFGRRPVLITSLFFLSINFLILGLADS--LWLLFLGRVLTGITAATYATASALIADVSPSSERAQNFGLMGIAFG---LGFICGPALGGVLGSWDVRAPFFAAAGLTLLNTMYGLFFL--QETLPK--------------------------EQRRT---FAWKRANPVGAMRQISRYPALSGLIACMFLYAIGHHVFPSNWNFYTIERFAWSPLDVGLSLAFVGVMAVAVQGGLLRWVIPKFGAERVACFGIGATILAYLGVASAASVT--TLYIWIAISSLGGLTGPAVQSIMTNQLPQNEQGELQGVIASVNSLAMILGPLAITQSFTYFTSPTTPIYLPSAAFLFAAAITVISLGIF---VRQASRLNRLLHSG 407          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A3D5S9N2_9BACT (Tetracycline resistance MFS efflux pump n=3 Tax=Bacteria TaxID=2 RepID=A0A3D5S9N2_9BACT)

HSP 1 Score: 98.2 bits (243), Expect = 5.250e-18
Identity = 104/415 (25.06%), Postives = 174/415 (41.93%), Query Frame = 2

Query:  167 ITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGL-GIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAA-LLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRP---KISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLDF-RFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLR----IIGEGVAAPIFSHTFSAGCTVRFEQAPFFLAAIFSFVSLTITW 1381
            I   I+  V P +I     G    AS + GWL  A ++ Q V  P++ ++SD  GRR V+  + +  G   ++L+    +L W  +  +V  +   +   + A + DI+A   +    G+ G A+G   L   VG +IGG L E         + GL +   L   FVL    +   R                                  RP   + +NP        +  +++ + +S  L  ++ +     + Y    +F WS  E+ Y  A    ++ALV G+ +R I+ + + +  G   G        +   +   GWML+  L   A   I  P LQ +M+  V  + QG LQG +TSL     ++G  +   IF +   A   V    APF+LAA+FSF ++ +++
Sbjct:   20 IGVGIIIPVVPALITELTGGSISDASIWGGWLMFAYALMQFVCAPIVGALSDQYGRRPVLLLSLLGFGLD-YILAGFAPTLGWLFLARIVAGITGASFTTANAYIADISAPEKRSQNFGMVGAAFG---LGFIVGPLIGGVLGEYGSRVPFFASAGLVLLNWLYGYFVLPESLSKENR----------------------------------RPFSWRRANPWGTLMQLKKYPMVIGLLVSLVLVYIAAHATQSTWTYFTIEKFDWSEAEVGYSLAFVGFMIALVQGVAVRPIINK-IGQARGVYVGLAFNCLGFILIAMSGTGWMLYLVLIPYAFGGIAGPSLQGLMSNSVPANEQGELQGGLTSLMSLTTVVGPPLMTNIFGYFTDASREVYIPSAPFYLAAVFSFTAMLMSY 395          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A4YT38_BRASO (Putative tetracycline-efflux transporter n=2 Tax=unclassified Bradyrhizobium TaxID=2631580 RepID=A4YT38_BRASO)

HSP 1 Score: 97.1 bits (240), Expect = 1.590e-17
Identity = 115/427 (26.93%), Postives = 180/427 (42.15%), Query Frame = 2

Query:  152 LFLSAITTSILSQVYPKIILLHFEGDDRKASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIA-GAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHG-LGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMA-ALLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYN-YLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFS----AGCTVRFEQAPFFLAAIFSFVSLTITWWPLREMDKR 1408
            + L  +   ++  + PK+I      D  +A+   G   TA ++ QLVF PVL S+SD  GRR VI    F L  AA +VL     SL W  +  L++ +   +I  + A + D+     + A  G +G A+GA  +   +G   GG L +         A GLS A AL  L VL     A +R A                                R + +NP+    +    R L  ++L  F+  L+  V    +  Y  +R+ W    +    A       +V G  + FIV R L E    + G        + +GL   G + +  +   AL  ++   +QA+M   V  DRQG LQGA +S++ + +     +F+ TF+    AG  +    APF LA+    V+L I    LR  +++
Sbjct:   35 ILLDMLALGLIMPILPKLIEGFVGNDTAQAARIFGLFGTAWALMQLVFSPVLGSLSDRFGRRPVILLSNFGL--AADYVLMALAPSLAWLFLGRLISGVTSASISTAFAYISDLTPPDRRAAVFGRIGAAFGAGFV---LGPATGGLLGDIDPRLPFWAAAGLSFANALYGLLVLPESLPAERRSA-------------------------------FRWRAANPLGALRLLRSERTLAGLSLVNFITQLAHVVLPSTFVLYATYRYGWDTRTVGLTLAIVGVCAMVVQGGAVGFIV-RRLGERGALMLGLSAGTVGFLIFGLAPTGMLSWTGIPAMALWGVSGAAIQALMTRLVPPDRQGQLQGATSSVQSMAQLAGPFLFTLTFAYFIGAGAPLHLPGAPFLLASALLIVALVIAVRALRAEERK 424          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A3D9AML1_9FLAO (Tetracycline resistance MFS efflux pump n=1 Tax=Chryseobacterium pennipullorum TaxID=2258963 RepID=A0A3D9AML1_9FLAO)

HSP 1 Score: 94.4 bits (233), Expect = 1.170e-16
Identity = 95/372 (25.54%), Postives = 160/372 (43.01%), Query Frame = 2

Query:  248 YRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGAFVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGLGIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALL-SLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLDFRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLR----IIGEGVAAPIFSHTFSAGCTVRFEQAPFFLAA 1348
            Y GW     ++ Q +F PVL  +SD  GRR V+  + +  G    +L+  P  LIW  +  ++  +   ++  + A + D+     +G   G+  A     + + +G +IGG L +         A GLS   LL   FVL     A KR                           R    K    I    S+    L   L+L  AL   + +   +V +    Y+  +F W    I Y       L+ +  G  I + + + + ++   +    +   + + +G+  RGWM+F  +AF AL  +  P LQA++++ V  D+QG LQG+++S+     I+G  +   +F++  +AG  V F  APF LAA
Sbjct:   49 YMGWFIAVYALMQFLFAPVLGGLSDRYGRRPVLLCSLLGLGIDYLILAMAPD-LIWLFVGRVIVGIMGSSVTTATAYISDVTEPEKRGKNFGMISAIAG--IGMIIGPVIGGVLGQYGERLPFYAAAGLSFLNLLYGFFVLPESLEATKR---------------------------RPFSWKTANPIGGMKSLNKEILAPTLILAFAL---IALAGQSVQSTWNIYVMEKFSWKEDMIGYSMGFLGLLIIVFQGGLIGYFLNK-VGQKKSVIIFLSVYCISLLLFGIANRGWMMFAVIAFYALGGMVSPILQALISSKVPDDKQGLLQGSLSSIMSITSIVGPLLMTSVFAYFTAAGSPVFFSGAPFILAA 386          

BLAST of mRNA_F-serratus_M_contig1528.3624.1 vs. uniprot
Match: A0A346YMY6_9HYPH (Tetracycline resistance MFS efflux pump n=2 Tax=Rhizobium/Agrobacterium group TaxID=227290 RepID=A0A346YMY6_9HYPH)

HSP 1 Score: 93.2 bits (230), Expect = 2.760e-16
Identity = 105/423 (24.82%), Postives = 175/423 (41.37%), Query Frame = 2

Query:  152 LFLSAITTSILSQVYPKIILLHFEGDDRK-ASTYRGWLATAVSVTQLVFVPVLCSVSDVVGRRAVIAGA--------FVLHGAAAFVLSTTPSSLIWATMLDLVTCLCIVNIPLSQAIMIDIAAGGGKGATHGL-GIAYGAHALALSVGDIIGGSLSERYRSQACLVAGGLSMAALLSLFVLGWEETAPKRVAASEEDDAESDAETTGSVDGSDCEQQRGTEGKIRPKISNPVSIFTVFLESRLLLQIALSYFLFILSLNVFAVGYNYLD-FRFHWSPLEISYFFATFNALMALVSGLCIRFIVPRHLSEENGALFGTCIQGCAGVAYGLCYRGWMLFPALAFGALQNITEPCLQAIMATFVAKDRQGSLQGAVTSLRIIGEGVAAPIFSHTFSA----GCTVRFEQAPFFLAAIFSFVSLTI 1375
            LFL  I  +I+  V P   L    GDD   A+   GWL    S  Q +F P+L ++SD  GRR ++  +         +   A +F +     +L   +     TC          A + DI+    +    GL GIA+G   +         G L E       L AG LS+A  ++ + L      P+ +                         ++        K +NP            +  + L  FL+ L+  V+   ++++  +R++WS  +I      F    A+V G  +  +VP  L E   A+ G        + Y   ++GW+++  +    ++N+ +P L++I +  V    QG LQGA+TSL  +   +   IF+  FSA    G  V F  AP+F+AAIF  +++T+
Sbjct:   18 LFLDIIGIAIIMPVLPTF-LRELTGDDVSGAAVDGGWLLLIYSAMQFLFAPLLGNLSDRFGRRPILLASVLTFAVDNLICAAATSFWMLFVGRTLAGISGGSFATC---------SAYIADISTDETRAKNFGLIGIAFG---VGXXXXXXXXGLLGELGPRAPFLAAGLLSLANFIAAWFL-----LPETL-------------------------EKANRRTFELKRANPFGALKQMRHYPGIGWVMLVMFLYWLAHAVYPSVWSFVSAYRYNWSEGQIGLSLGMFGICAAIVMGFVLPKLVP-VLGEWRTAVLGLSFSCLGLLGYAFAWQGWVVYAVILLTTIENVGDPALRSIASAKVPASAQGELQGAITSLTSLTTIIGPAIFTQLFSAFTGEGAAVEFAGAPYFMAAIFMALAITV 396