mRNA_F-distichus_contig103745.334.1 (mRNA) Fucus distichus monoicous

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_F-distichus_contig103745.334.1
Unique NamemRNA_F-distichus_contig103745.334.1
TypemRNA
OrganismFucus distichus monoicous (Fucus distichus monoicous)
Homology
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: D7G161_ECTSI (Alpha-1,3-glucosyltransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G161_ECTSI)

HSP 1 Score: 187 bits (474), Expect = 3.020e-52
Identity = 117/205 (57.07%), Postives = 132/205 (64.39%), Query Frame = 1 
Query:    4 GALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYCCDMTRVTNASSSSSSGGGRISNESDGRGETSQGSGTNPYQLPGAPPPSG----VFVAERSESARRPEEASRPCGIGGKRVVLFFRRLAALGSVVLVVFGAALGPLCVSEGWTGDACARQLGQLAVRLFPFGR 606
            G LVLLNSGL +VDHVHFQYNGFLLG+LLLSMG IRQGRVL G A FA LLMLKHLFL+LAPLYFV+LLR YCC  +    +     SG  R   ES   GE SQ            PP  G    +  A  S S  + +  SR          L ++RLA+LGS VL VFG+ LGPLCVS+GWT DAC +QLGQL VRLFPFGR
Sbjct:  140 GPLVLLNSGLFLVDHVHFQYNGFLLGLLLLSMGLIRQGRVLSGGATFAGLLMLKHLFLALAPLYFVYLLRSYCCGASAQQLSKGVGGSGDVRRGGESS-VGEASQ------------PPERGDRKDLQRAVPSVSHEQVQAVSRE---DAPLARLSWKRLASLGSAVLFVFGSVLGPLCVSDGWTKDACLKQLGQLGVRLFPFGR 328
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: W7UBL8_9STRA (Alpha-1,3-glucosyltransferase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7UBL8_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 1.450e-19
Identity = 48/74 (64.86%), Postives = 59/74 (79.73%), Query Frame = 1 
Query:    1 LGALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            L  + L ++GL+MVDH+HFQYNG LLG+L LSM  +R+G +L GA  FASLLM KHLFLSLAP+YFV+LL  YC
Sbjct:  137 LAMMTLASAGLLMVDHMHFQYNGMLLGILFLSMAALRKGWILAGAGLFASLLMFKHLFLSLAPVYFVYLLSSYC 210
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A0G4ENQ5_VITBC (Alpha-1,3-glucosyltransferase n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4ENQ5_VITBC)

HSP 1 Score: 96.3 bits (238), Expect = 1.620e-19
Identity = 50/76 (65.79%), Postives = 64/76 (84.21%), Query Frame = 1 
Query:    1 LGALV--LLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            +G L+  +LN GL++VDHVHFQYNG +LGVLLLSM  I +GRVL+G+A FA LL++KH+FL +AP+YFVFLLR YC
Sbjct:  184 IGGLISCVLNPGLLIVDHVHFQYNGMMLGVLLLSMAQIMRGRVLVGSALFALLLIMKHIFLYIAPVYFVFLLRTYC 259
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A7S3YXA4_9EUKA (Alpha-1,3-glucosyltransferase (Fragment) n=1 Tax=Lotharella globosa TaxID=91324 RepID=A0A7S3YXA4_9EUKA)

HSP 1 Score: 91.7 bits (226), Expect = 4.410e-19
Identity = 45/70 (64.29%), Postives = 57/70 (81.43%), Query Frame = 1 
Query:   13 VLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            V+LN GL++VDH+HFQYNGFL+G+LLLS+  IR+G  LLG   FA LL  KH+FL +APLYF++LLR YC
Sbjct:   18 VVLNPGLLLVDHIHFQYNGFLMGILLLSLAAIRRGEDLLGGFLFAVLLNFKHIFLYVAPLYFIYLLRRYC 87
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A1B6M1M6_9HEMI (Alpha-1,3-glucosyltransferase n=4 Tax=Cicadellinae TaxID=33370 RepID=A0A1B6M1M6_9HEMI)

HSP 1 Score: 94.4 bits (233), Expect = 6.360e-19
Identity = 52/83 (62.65%), Postives = 62/83 (74.70%), Query Frame = 1 
Query:    1 LGALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYCCDMTRVTNA 249
            L  L+L N+GLI+VDH+HFQYNGFL GVLLLS+  I QGR L GA  FA LL LKH+F+ +AP Y V+LLR YC D T+  NA
Sbjct:  138 LQLLLLGNAGLIIVDHIHFQYNGFLFGVLLLSIARILQGRCLEGAYWFAVLLNLKHIFVYIAPAYGVYLLRNYCFDRTKEANA 220
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: UPI00098E7427 (LOW QUALITY PROTEIN: probable dolichyl pyrophosphate Glc1Man9GlcNAc2 alpha-1,3-glucosyltransferase n=1 Tax=Asparagus officinalis TaxID=4686 RepID=UPI00098E7427)

HSP 1 Score: 93.2 bits (230), Expect = 1.300e-18
Identity = 46/72 (63.89%), Postives = 59/72 (81.94%), Query Frame = 1 
Query:    7 ALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            ALVL +  L++VDHVHFQYNGFLLG+LL+S+GF+R+GR L G  AFA L+  KHLF+  AP+YF++LLR YC
Sbjct:   87 ALVLWSPALLIVDHVHFQYNGFLLGILLISLGFLREGRDLAGGFAFAVLICSKHLFMVAAPVYFIYLLRRYC 158
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A835Z7Q6_9STRA (Dolichyl-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichol alpha-1,3-glucosyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z7Q6_9STRA)

HSP 1 Score: 93.6 bits (231), Expect = 1.460e-18
Identity = 46/71 (64.79%), Postives = 57/71 (80.28%), Query Frame = 1 
Query:   10 LVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            LV LN GL++ DH+HFQYNG LLGVLLLS+G + QGR +     FA+LLM+KHL+  LAPLYFV+LL+GYC
Sbjct:  106 LVTLNGGLLLADHMHFQYNGMLLGVLLLSLGLMIQGRQVASGLVFAALLMMKHLYACLAPLYFVYLLKGYC 176
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A182FQD7_ANOAL (Alpha-1,3-glucosyltransferase n=7 Tax=Nyssorhynchus TaxID=44543 RepID=A0A182FQD7_ANOAL)

HSP 1 Score: 93.2 bits (230), Expect = 1.540e-18
Identity = 48/73 (65.75%), Postives = 60/73 (82.19%), Query Frame = 1 
Query:    4 GALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYC 222
            GAL+L N+GL+MVDH+HFQYNGFL GVLLLS+G + +GR L  AA FA LL LKH+F+ +AP+Y V+LLR YC
Sbjct:  132 GALLLGNAGLLMVDHIHFQYNGFLFGVLLLSIGALLEGRPLQSAALFAVLLNLKHIFIYVAPVYVVYLLRFYC 204
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A8J4X2_CHLRE (Alpha-1,3-glucosyltransferase (Fragment) n=2 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A8J4X2_CHLRE)

HSP 1 Score: 92.8 bits (229), Expect = 2.160e-18
Identity = 50/87 (57.47%), Postives = 65/87 (74.71%), Query Frame = 1 
Query:    1 LGALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYCCDMTRVTNASSSS 261
            L AL+ LN+GL++VDH+HFQYNG ++GVLLLS+   R GR LL    FA LL +KHLFL  AP+YFV+LLR YC + T  +++S SS
Sbjct:  124 LFALLALNAGLLLVDHIHFQYNGAMMGVLLLSLAAARTGRHLLSGVLFAVLLNMKHLFLFAAPVYFVYLLRHYCFETTGGSSSSGSS 210
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BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Match: A0A6G1FAK9_9ORYZ (Alpha-1,3-glucosyltransferase n=1 Tax=Oryza meyeriana var. granulata TaxID=110450 RepID=A0A6G1FAK9_9ORYZ)

HSP 1 Score: 91.3 bits (225), Expect = 2.320e-18
Identity = 47/73 (64.38%), Postives = 55/73 (75.34%), Query Frame = 1 
Query:    7 ALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFASLLMLKHLFLSLAPLYFVFLLRGYCC 225
            ALVL +  L+ VDHVHFQYNGFLLG+LLLS+ F+ QGR L G   FA+LL  KHLFL   P+YFV+L R YCC
Sbjct:  138 ALVLWSPALLAVDHVHFQYNGFLLGLLLLSLHFLEQGRDLAGGVVFAALLCSKHLFLVAVPVYFVYLFRHYCC 210
The following BLAST results are available for this feature:
BLAST of mRNA_F-distichus_contig103745.334.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7G161_ECTSI3.020e-5257.07Alpha-1,3-glucosyltransferase n=2 Tax=Ectocarpus T... [more]
W7UBL8_9STRA1.450e-1964.86Alpha-1,3-glucosyltransferase n=2 Tax=Monodopsidac... [more]
A0A0G4ENQ5_VITBC1.620e-1965.79Alpha-1,3-glucosyltransferase n=3 Tax=Vitrella bra... [more]
A0A7S3YXA4_9EUKA4.410e-1964.29Alpha-1,3-glucosyltransferase (Fragment) n=1 Tax=L... [more]
A0A1B6M1M6_9HEMI6.360e-1962.65Alpha-1,3-glucosyltransferase n=4 Tax=Cicadellinae... [more]
UPI00098E74271.300e-1863.89LOW QUALITY PROTEIN: probable dolichyl pyrophospha... [more]
A0A835Z7Q6_9STRA1.460e-1864.79Dolichyl-P-Glc:Glc(1)Man(9)GlcNAc(2)-PP-dolichol a... [more]
A0A182FQD7_ANOAL1.540e-1865.75Alpha-1,3-glucosyltransferase n=7 Tax=Nyssorhynchu... [more]
A8J4X2_CHLRE2.160e-1857.47Alpha-1,3-glucosyltransferase (Fragment) n=2 Tax=C... [more]
A0A6G1FAK9_9ORYZ2.320e-1864.38Alpha-1,3-glucosyltransferase n=1 Tax=Oryza meyeri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
F-distichus_contig103745contigF-distichus_contig103745:378..1691 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score190.7
Seed ortholog evalue7e-46
Seed eggNOG ortholog2880.D7G161
Preferred nameALG8
KEGG koko:K03849
KEGG ReactionR06263
KEGG Pathwayko00510,ko01100,map00510,map01100
KEGG ModuleM00055
Hectar predicted targeting categoryother localisation
GOsGO:0000030,GO:0000033,GO:0000902,GO:0000904,GO:0001703,GO:0002064,GO:0003381,GO:0003382,GO:0003383,GO:0003384,GO:0003674,GO:0003824,GO:0004583,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0006464,GO:0006486,GO:0006487,GO:0006488,GO:0006490,GO:0006629,GO:0006807,GO:0006928,GO:0007275,GO:0007369,GO:0007377,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009653,GO:0009790,GO:0009888,GO:0009987,GO:0010004,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0016740,GO:0016757,GO:0016758,GO:0019538,GO:0030029,GO:0030048,GO:0030154,GO:0030855,GO:0031224,GO:0031984,GO:0032501,GO:0032502,GO:0032989,GO:0034645,GO:0036211,GO:0042175,GO:0042281,GO:0042283,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043413,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0046527,GO:0048468,GO:0048598,GO:0048856,GO:0048869,GO:0060429,GO:0070085,GO:0070252,GO:0071704,GO:0071840,GO:0097502,GO:0098827,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity
EggNOG OGsKOG2576@1,KOG2576@2759
Ec32 ortholog descriptionDolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl glucosyltransferase ALG8, family GT57
Ec32 orthologEc-22_000030.1
EC2.4.1.265
COG Functional cat.M
CAZyGT57
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Exons2
Model size621
Cds size621
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622928957.1577823-CDS-F-distichus_contig103745:377..4911622928957.1577823-CDS-F-distichus_contig103745:377..491Fucus distichus monoicousCDSF-distichus_contig103745 378..491 +
1690885081.6392524-CDS-F-distichus_contig103745:377..4911690885081.6392524-CDS-F-distichus_contig103745:377..491Fucus distichus monoicousCDSF-distichus_contig103745 378..491 +
1622928957.169204-CDS-F-distichus_contig103745:1184..16911622928957.169204-CDS-F-distichus_contig103745:1184..1691Fucus distichus monoicousCDSF-distichus_contig103745 1185..1691 +
1690885081.6619062-CDS-F-distichus_contig103745:1184..16911690885081.6619062-CDS-F-distichus_contig103745:1184..1691Fucus distichus monoicousCDSF-distichus_contig103745 1185..1691 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_F-distichus_contig103745.334.1prot_F-distichus_contig103745.334.1Fucus distichus monoicouspolypeptideF-distichus_contig103745 378..1691 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_F-distichus_contig103745.334.1

>prot_F-distichus_contig103745.334.1 ID=prot_F-distichus_contig103745.334.1|Name=mRNA_F-distichus_contig103745.334.1|organism=Fucus distichus monoicous|type=polypeptide|length=207bp
LGALVLLNSGLIMVDHVHFQYNGFLLGVLLLSMGFIRQGRVLLGAAAFAS
LLMLKHLFLSLAPLYFVFLLRGYCCDMTRVTNASSSSSSGGGRISNESDG
RGETSQGSGTNPYQLPGAPPPSGVFVAERSESARRPEEASRPCGIGGKRV
VLFFRRLAALGSVVLVVFGAALGPLCVSEGWTGDACARQLGQLAVRLFPF
GRRGGK*
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mRNA from alignment at F-distichus_contig103745:378..1691+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_F-distichus_contig103745.334.1 ID=mRNA_F-distichus_contig103745.334.1|Name=mRNA_F-distichus_contig103745.334.1|organism=Fucus distichus monoicous|type=mRNA|length=1314bp|location=Sequence derived from alignment at F-distichus_contig103745:378..1691+ (Fucus distichus monoicous)
CTGGGGGCGCTCGTGCTTCTGAACTCCGGCCTGATTATGGTGGACCACGT
GCACTTCCAGTACAACGGGTTTCTACTGGGCGTGCTGCTGCTTTCGATGG
GTTTCATCCGACAGGTCCGACCTCGATCGATCCAAACCTGTATAGGCTTC
GACAACACCGATGGCCTCCACCCACCGTGCTGTGCTGTAACAAACGCACA
CATACAGCCGTTTAACGAAAGGGGCGTGGAAGATTTCTAAGGGTTTCATG
ATCCCGTGCCCAATTTCATGGGGGGGGGGGCGTCACCAAACAGCCCCTCC
TGGGGATGGGACTGAAAGTTTCTATAGACCTGATAGGCAAGCTTGCCCCA
AACTCAACTCATTTTTACTCGGGGTTTGGTGAAATCTGTACTCGATAATT
TTTTGCGCCTCCCCCCCTGAACTCGGGGGACAGTTCTGCGACCCCCCTCC
ATCCCCCCATACTCCTTTCGTTGACCGATTCCATACAGAGTGATATGTTT
TTTTTGTGCTTCAATATTATAAATACAAGACGTGTCGTGTTGTCGTGGCG
CGGGTAGGATCTTAGCCGTTCTCGTGGGTGAGGCGCAGCGGGTTAGCACG
CCTCAATCATCGCGGGTGGTCCTCGCTGGCTTCAAGCGAAGGCCTCACTG
AACGCGTCAGACTCAACCTGACTCATAAAAGTAAAGAAAATAGCTGTTCG
TCGTCTATCTCTCGAGAGCGACAGTCCACCTTCAAACGGTCCACTCCTTC
CTTCCCCCCTCCCCTCTCTGGCGCGCCCTCTTCGTTTTCATTTCTCGATG
CGACCAGGGCAGGGTTTTGCTAGGGGCCGCGGCGTTCGCGAGCCTGCTCA
TGCTCAAGCACCTCTTCTTGAGCCTCGCGCCCCTGTACTTTGTGTTCCTC
TTGAGAGGCTACTGCTGCGACATGACGCGCGTCACCAATGCCAGCAGCAG
CAGCAGCAGCGGCGGCGGTAGAATCAGCAACGAAAGTGATGGGCGTGGTG
AAACTTCGCAGGGCTCGGGGACAAACCCATATCAGCTTCCAGGAGCTCCC
CCCCCCAGCGGAGTCTTCGTGGCCGAGCGCTCCGAGTCTGCCCGTCGTCC
GGAGGAAGCGTCACGACCTTGCGGGATCGGAGGGAAACGCGTCGTCCTCT
TCTTCCGTCGGCTCGCCGCCCTGGGCTCGGTGGTTTTGGTGGTCTTCGGT
GCGGCGCTTGGGCCGCTGTGCGTCTCCGAGGGCTGGACCGGGGACGCGTG
CGCGAGGCAACTCGGCCAGCTCGCGGTCAGGCTCTTCCCATTCGGCAGGC
GAGGGGGAAAATAA
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Coding sequence (CDS) from alignment at F-distichus_contig103745:378..1691+

>mRNA_F-distichus_contig103745.334.1 ID=mRNA_F-distichus_contig103745.334.1|Name=mRNA_F-distichus_contig103745.334.1|organism=Fucus distichus monoicous|type=CDS|length=1242bp|location=Sequence derived from alignment at F-distichus_contig103745:378..1691+ (Fucus distichus monoicous)
CTGGGGGCGCTCGTGCTTCTGAACTCCGGCCTGATTATGGTGGACCACGT
GCACTTCCAGTACAACGGGTTTCTACTGGGCGTGCTGCTGCTTTCGATGG
GTTTCATCCGACAGCTGGGGGCGCTCGTGCTTCTGAACTCCGGCCTGATT
ATGGTGGACCACGTGCACTTCCAGTACAACGGGTTTCTACTGGGCGTGCT
GCTGCTTTCGATGGGTTTCATCCGACAGGGCAGGGTTTTGCTAGGGGCCG
CGGCGTTCGCGAGCCTGCTCATGCTCAAGCACCTCTTCTTGAGCCTCGCG
CCCCTGTACTTTGTGTTCCTCTTGAGAGGCTACTGCTGCGACATGACGCG
CGTCACCAATGCCAGCAGCAGCAGCAGCAGCGGCGGCGGTAGAATCAGCA
ACGAAAGTGATGGGCGTGGTGAAACTTCGCAGGGCTCGGGGACAAACCCA
TATCAGCTTCCAGGAGCTCCCCCCCCCAGCGGAGTCTTCGTGGCCGAGCG
CTCCGAGTCTGCCCGTCGTCCGGAGGAAGCGTCACGACCTTGCGGGATCG
GAGGGAAACGCGTCGTCCTCTTCTTCCGTCGGCTCGCCGCCCTGGGCTCG
GTGGTTTTGGTGGTCTTCGGTGCGGCGCTTGGGCCGCTGTGCGTCTCCGA
GGGCTGGACCGGGGACGCGTGCGCGAGGCAACTCGGCCAGCTCGCGGTCA
GGCTCTTCCCATTCGGCAGGCGAGGGGGAAAATAAGGCAGGGTTTTGCTA
GGGGCCGCGGCGTTCGCGAGCCTGCTCATGCTCAAGCACCTCTTCTTGAG
CCTCGCGCCCCTGTACTTTGTGTTCCTCTTGAGAGGCTACTGCTGCGACA
TGACGCGCGTCACCAATGCCAGCAGCAGCAGCAGCAGCGGCGGCGGTAGA
ATCAGCAACGAAAGTGATGGGCGTGGTGAAACTTCGCAGGGCTCGGGGAC
AAACCCATATCAGCTTCCAGGAGCTCCCCCCCCCAGCGGAGTCTTCGTGG
CCGAGCGCTCCGAGTCTGCCCGTCGTCCGGAGGAAGCGTCACGACCTTGC
GGGATCGGAGGGAAACGCGTCGTCCTCTTCTTCCGTCGGCTCGCCGCCCT
GGGCTCGGTGGTTTTGGTGGTCTTCGGTGCGGCGCTTGGGCCGCTGTGCG
TCTCCGAGGGCTGGACCGGGGACGCGTGCGCGAGGCAACTCGGCCAGCTC
GCGGTCAGGCTCTTCCCATTCGGCAGGCGAGGGGGAAAATAA
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