EsuBft1254_7a-0001 (mRNA) Ectocarpus subulatus male Bft15b

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	EsuBft1254_7a-0001
Unique Name	EsuBft1254_7a-0001
Type	mRNA
Organism	Ectocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Scaffold_195	contig	Scaffold_195:652100..656506 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
OGS1.0 of Ectocarpus subulatus male Bft15b	2020-06-19

Properties


Property Name	Value
Seed ortholog	2880.D8LPZ1
ProteomeId	EsuBft1254_7
Protein domains	CYTOPLASMIC_DOMAIN
Protein domains	TMhelix
Protein domains	Coil
Protein domains	TRANSMEMBRANE
Protein domains	NON_CYTOPLASMIC_DOMAIN
Owner	apollo_admin@sb-roscoff.fr
Nb exon	5
Max annot lvl	2759\|Eukaryota
Length	195
Hectar predicted targeting category	other localisation
Evalue	1.99e-99
EggNOG OGs	KOG3676@1\|root,KOG3676@2759\|Eukaryota
Date last modified	2016-06-14
Date creation	2016-06-14
Description	calcium ion transmembrane transport
COG category	U

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
EsuBft1254_7a	EsuBft1254_7a	Ectocarpus subulatus male Bft15b	gene	Scaffold_195 652100..656506 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft1254_7a-0001	EsuBft1254_7	Ectocarpus subulatus male Bft15b	polypeptide	Scaffold_195 652100..656506 +

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft1254_7a-0001-exon	agat-exon-9734	Ectocarpus subulatus male Bft15b	exon	Scaffold_195 652100..652107 +
EsuBft1254_7a-0001-exon	agat-exon-9735	Ectocarpus subulatus male Bft15b	exon	Scaffold_195 653617..654553 +
EsuBft1254_7a-0001-exon	agat-exon-9736	Ectocarpus subulatus male Bft15b	exon	Scaffold_195 655403..655580 +
EsuBft1254_7a-0001-exon	EsuBft1254_7a-0001-exon	Ectocarpus subulatus male Bft15b	exon	Scaffold_195 655638..656147 +
EsuBft1254_7a-0001-exon	agat-exon-9733	Ectocarpus subulatus male Bft15b	exon	Scaffold_195 656190..656506 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft1254_7a-0001-cds	EsuBft1254_7a-0001-cds	Ectocarpus subulatus male Bft15b	CDS	Scaffold_195 652100..652107 +

Sequences

The following sequences are available for this feature:

mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at Scaffold_195:652100..656506+
coding sequence from alignment at Scaffold_195:652100..656506+

spliced messenger RNA

>EsuBft1254_7a-0001 ID=EsuBft1254_7a-0001|Name=EsuBft1254_7a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=1950bp|location=Sequence derived from alignment at Scaffold_195:652100..656506+ (Ectocarpus subulatus male Bft15b)|Notes=Excludes all bases but those of type(s): exon.  
ATGACCAGCGCTCAATTTTCAATATGGGCTAGTGCTGGACTTGGCAAGTG
GGACGGTATCGCACTGCTTCTGAAGAATTATGCATGTCCGTCTCCTGAAG
ACCTCATTGATCTCTCTCGCAAGCACCCTTCTGACGAGCGGCTGCACGAC
TGCTGTTTGAGAGTTGTAGCCGCGTCAGACAACCTGTTCATTTCCGGCAT
GACTTTGTCTGTGGCACGCGTGGATGTGACGAAGAAAGCTCCAGAGGGAG
AGAGGGGTACCCTTGAGGCCATCCAGGGCCATGTACATGCCTTACTGCTT
GAAATTCTCGAACGCTTGCCTCAAACAGTCAATGGTTTCAACGGAGGAAT
GGCCGACTGCTCAGCCGTGTTAGGGCCAGAGTTCGACAGCAAAACGGGAG
AATCTACGGGCTTCAAGCCACCGCTGGCCCTGCTCCTCCTGGCGAACAGT
ATTCAGACGGAAACGTTTTGCTCGACGCCCTTAATCATGGACTACTTGTC
ACGGAAATTCACGCATGGTCTGCCCGACGTGGGCGACAAGGACGGCGTGC
TTCGGAATGGCACAGAACATGATGTGCTGAGCGGCAGTGGGCCAGGCATG
ATGTCCCTGGTCATTGGTGGTGGCGATGACGAAGAACTGGACGAAAGAGG
TTATCACGAATTCCGACACAAGGATCTTCTGGTCAAGAATGGCCTAAAGG
CCAGCTTTGAATCCCCTCGTGCGTTGCTTCAGGGAGCGAATGATAGGCTT
GCCAGCCTGACGTTTCTTCCTGGGGCTCAATTTGCGATCGCAGGATTTCT
GGCGAAGCCTAACAACTACTATCGAATCCCCGCTGTGAGGATGGTGCTAG
ACGTTGTGGTGTACCTCTTGGTGATCATTGCGTTGAGCATCCTGGTCTTC
CGCGACAGCGGGTCGCTGACACGGGGAGAGGGCATCGCCGCGGCTGTTAT
TTTCGCGATGACGGCTCAGCTCGTGAGGTTCGGGATTGTGATGTTGGTTG
TCATGGTCGGATTCGCCATGTCCCTGCACGTGCTGATTCGCGACTCCAAA
AGCTTTGGTGAAACGTTTGTCGCACTATTCAAAGCGACGCTTGGTGACGT
GGACTACTTCACGGCGTCCTCTGTGCTCAACCTGCTGGTTGCGATATTGA
CCGTATCGCACTCCAAGGTACAGGAGAACGTTCAACGGGAGTTCAAGGTT
TCGAAGGCGCGCATGATCCAGCGATGCCGATGGACTGTAGAAAACGACTG
GCTTCCTGCCCCGTTCAACGTGGCACAGCTACTTTTCTCGGGGCAATACG
CGTTGGTGATGCCCAGCTTTCGGCGGAAACGGTATGATGAAGCAAAGCAA
GCCATTGGTCAGGCAGTGTCCTGGTTTGTGCTCGGTCTAATGGCTTTTGT
GGGGGGATCGATTCTCTGGATATTGTCGGTTGTGTACGCACTCTTTGTGT
GGCATGAGCACTACTACGTAGGGCTAGACACGCATACTGCTCACGTTATA
ATACTTCAAACTGAGCCTGGGAGCAACGATACTGCGGTATATCCTTATCC
CTGTGTGGTGCGTCTTGGATGCTCCGTTGTGTCGTTTCCTGCTCGGGGTC
AAGACGCTTCTACTCGGGCAAGTAGCGGGGGAGATGGCATGCTTAAGAGT
GCCGATCAGGGAGTAGGAATGGCAAAATTGCGCGAATTCCTCGCCGACCC
AATGAACAACAGAGATGTTCGTAAGGCCGGGACGACAAGGAGCACCACCG
TGGAACACCTTAAACTTCTTAGGGACCGCCTAGTGAAAGACATTGTAGGT
AGGCTCTGCAGACTTTTGAGAAAAGGGAAGGCATGGCCCAAACACTTGAT
TGAGGCTTTCGATCAGCGCTTGCAACAACTCGAAGCAAAGGTTGCCGAGA
CCAATGATTACCTAATTGAAATTTTGAAGTTCGTCAAGAACCCACGGTGA

protein sequence of EsuBft1254_7a-0001

>EsuBft1254_7 ID=EsuBft1254_7|Name=EsuBft1254_7a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=649bp
MTSAQFSIWASAGLGKWDGIALLLKNYACPSPEDLIDLSRKHPSDERLHD
CCLRVVAASDNLFISGMTLSVARVDVTKKAPEGERGTLEAIQGHVHALLL
EILERLPQTVNGFNGGMADCSAVLGPEFDSKTGESTGFKPPLALLLLANS
IQTETFCSTPLIMDYLSRKFTHGLPDVGDKDGVLRNGTEHDVLSGSGPGM
MSLVIGGGDDEELDERGYHEFRHKDLLVKNGLKASFESPRALLQGANDRL
ASLTFLPGAQFAIAGFLAKPNNYYRIPAVRMVLDVVVYLLVIIALSILVF
RDSGSLTRGEGIAAAVIFAMTAQLVRFGIVMLVVMVGFAMSLHVLIRDSK
SFGETFVALFKATLGDVDYFTASSVLNLLVAILTVSHSKVQENVQREFKV
SKARMIQRCRWTVENDWLPAPFNVAQLLFSGQYALVMPSFRRKRYDEAKQ
AIGQAVSWFVLGLMAFVGGSILWILSVVYALFVWHEHYYVGLDTHTAHVI
ILQTEPGSNDTAVYPYPCVVRLGCSVVSFPARGQDASTRASSGGDGMLKS
ADQGVGMAKLREFLADPMNNRDVRKAGTTRSTTVEHLKLLRDRLVKDIVG
RLCRLLRKGKAWPKHLIEAFDQRLQQLEAKVAETNDYLIEILKFVKNPR

mRNA from alignment at Scaffold_195:652100..656506+

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>EsuBft1254_7a-0001 ID=EsuBft1254_7a-0001|Name=EsuBft1254_7a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=4407bp|location=Sequence derived from alignment at Scaffold_195:652100..656506+ (Ectocarpus subulatus male Bft15b)
ATGACCAGGTAAGTGGTCGTGTAGGTCATCACGTGTGCTATGGTCAACTA
TCGTAATGAATGAGCGCCCGCAACTCGTGCGGATGGGTGCGGGCTCGTAG
CTCGCGTCGTTCATCATCTTATGACCAGGACACATCTAATGAGTTTGCCA
TGACCCTGGTGATCTCACCTCCGAGCACCTTGTGTTCCCGACCCAAGAGA
CTACCTTGTCTGGTTTTCGATAGGTGAGCCAGTCCATCGTGATACTCTGT
ATTCGCGTCGGCAAATGATCTTGAGCCAATCGACCTGGTTGGCGCAGCTT
GTGGTTGTTTCGAAAGTGCTCGGTCCAAAACACCCTCGCTCCGTCGATCG
CTCATGGGCACGTTTCATATCTCGTCGCCTTGGGTGAGCTTTTTTTGTCC
CTCCCGATTTGCTTGGTTCTGGCCGGTCTAGTTTACCAGTAGGTCAGTTT
CCTCCACAAGAGGTTTCTCAATCACCGGCCCGTACTGTAGTTGTTCGTCA
TAGTTTGAATGTATCGAAGGAGCCCCACAAGCGCTGGCTACAATCGTCCC
GTCGCGTGACACGTGCACTGAAGTTTGTTCCCGCTCGCCATGAGCTCCAA
GTGGTGAATGTATCCGTCAACGTTGAATGATGTGAGGGACCATTCGAGGG
TCGGAATAACCGAAAGGCAGCGTTTGCACATCGATGGAGGCCCTTCGCTA
TCCCCCGTACGCCATGCAGCTCATTGAACTGATCATGGCAAGGGAGGGTT
TCGGCATGTCACTCCTGGGTTGCACCGTCGTTACAAGACGCCGTCACTAT
GGATATTCCAAGGATACTTTATGTAAGACTGTTTTTTCTTTCTTTTTTCC
ATGATAGTCGACAGAGAGGTATCTCTACTGGCAACGTCACCGTCAGGCGG
GTGCGAAATGGTTTGCCAGATTGTTGAAACCTAACAAAGTGAAGGAGAGT
CCTCCGGTGTGGTAGGAGCGTCGGGAACATCCTTTGGACAAAGGTTTCTC
TTGTGGATACGAGTGCTTTGTTTCGTCTATGATGTTTGTGGAAAACTTGA
ATCAACGCGTCCCGGATTCTTGAACGTGTGAAAGTTACTTCGAGCACGGT
GGTTCCGCTTTATTTCTTTCAGACTTGCTGCGCCATGCGCCTTACCTTGT
ATTGTAGGATCTTCCGACTATGTCCTGGTGGACGCAGATTTGATTCAGTT
TCCTATCTATTTTGGATGGTATGAAGGTCATGTCTCAAGGTGAACAAAAA
TACAATGAACTGATGGTGACAAAAATCGTCTGCTCGCGCAATCGGGCGAA
ATATTGACTACATCGTAGTGAGGCTTTTGCTCTCGCTTTCGCATCTTCTT
ATTGTGGGGTATTGTGAATCAAGTCGCTCCATTTGGTGGCTGCGCCTTCT
CACGGAGATCGGCACGGTGCCAGGTTTTTGCTCATGACCAAGATACCATT
CCCGCACGGAGTGTCGGTCCCCTTTTCCACCTTTAGTTGTGCAACTGCAA
GTGGCAGACGAACTCAGCGCTCAATTTTCAATATGGGCTAGTGCTGGACT
TGGCAAGTGGGACGGTATCGCACTGCTTCTGAAGAATTATGCATGTCCGT
CTCCTGAAGACCTCATTGATCTCTCTCGCAAGCACCCTTCTGACGAGCGG
CTGCACGACTGCTGTTTGAGAGTTGTAGCCGCGTCAGACAACCTGTTCAT
TTCCGGCATGACTTTGTCTGTGGCACGCGTGGATGTGACGAAGAAAGCTC
CAGAGGGAGAGAGGGGTACCCTTGAGGCCATCCAGGGCCATGTACATGCC
TTACTGCTTGAAATTCTCGAACGCTTGCCTCAAACAGTCAATGGTTTCAA
CGGAGGAATGGCCGACTGCTCAGCCGTGTTAGGGCCAGAGTTCGACAGCA
AAACGGGAGAATCTACGGGCTTCAAGCCACCGCTGGCCCTGCTCCTCCTG
GCGAACAGTATTCAGACGGAAACGTTTTGCTCGACGCCCTTAATCATGGA
CTACTTGTCACGGAAATTCACGCATGGTCTGCCCGACGTGGGCGACAAGG
ACGGCGTGCTTCGGAATGGCACAGAACATGATGTGCTGAGCGGCAGTGGG
CCAGGCATGATGTCCCTGGTCATTGGTGGTGGCGATGACGAAGAACTGGA
CGAAAGAGGTTATCACGAATTCCGACACAAGGATCTTCTGGTCAAGAATG
GCCTAAAGGCCAGCTTTGAATCCCCTCGTGCGTTGCTTCAGGGAGCGAAT
GATAGGCTTGCCAGCCTGACGTTTCTTCCTGGGGCTCAATTTGCGATCGC
AGGATTTCTGGCGAAGCCTAACAACTACTATCGAATCCCCGCTGTGAGGA
TGGTGCTAGACGTTGTGGTGTACCTCTTGGTGATCATTGCGTTGAGCATC
CTGGTCTTCCGCGACAGCGGGTCGCTGACACGGGGAGAGGGCATCGCCGC
GGCTGTTTCATAGCGGTGAGTTGAGGTCGCGGGCGGGCGGTCAACCACTG
TTGTTGCCAGCGCACTCTCCAGGTTCGATGTGTGGGCACTTCGCCGACTC
CTTTATTCGCGAAACCCGTTGAGGTCGGTTTTTGTTTCGATTCTTTTTTG
ACTGCAGCCAAATATTCGTGCGGCTGCGTCCGAGCCTCGGTCATGGCCTC
GTTGTGCTTGGGAAATTAAACCGCAAACTACCAGATTGTTTGTTTCGCGG
CTCATCGGCTTATTCTCGCTTCCGTGCGTACCTCTCCAAAAATCTTGCCG
GGTCGGCCGTTTCAGACAAGAACTTTGAGAGAGAAGCGCGAGCTTCACAA
CTGCGTCAAGGAACCTGGTGCCTTGTGGACCGGTGTAGGGGTATTCTTCC
AAGACCCATGGAACGCTGTTGACGTGATGGGGTGGCAGCTCCAAGTGCCG
GCTTGGTCGTCCGCGGCTTAGATGGTGCCAGTTCAATGGGGCAAGGGTTG
TACGCCCTAAGTGCTCCGCTGTTCGTTGCACGCGTCCTCTTTTTCGCGCA
GATCCTCCCTTCCCAAAGACCAATGATTCAGGCAAGCGGCTATCGTATAC
CTGTCTGTGTTTCTCTCGGACCGCCTTCGTTGTTGATGTTGTTCAAAGTG
GATCAGACCTGAAATTCCGTCGCCTTTATGCGTTTGGAGGACGTGCGGCC
TCCGATCACTTCCCATGGCGGCAAAGGCAGCAAGGGGAAAAGGCACAACG
GTCCATTGGTTTTCGTAGCAGTAGCAGTTATGATGTCTTGGAATGTATGA
GCATCTCGCAATCCAATGGCCACCACGATGTCCCCTTCGCACTCTTGGCG
CAGGTTATTTTCGCGATGACGGCTCAGCTCGTGAGGTTCGGGATTGTGAT
GTTGGTTGTCATGGTCGGATTCGCCATGTCCCTGCACGTGCTGATTCGCG
ACTCCAAAAGCTTTGGTGAAACGTTTGTCGCACTATTCAAAGCGACGCTT
GGTGACGTGGACTACTTCACGGCGTCCTCTGGTGGCCGGCACGAACGGTG
GGGACAATTCTTATCGTGACATCTGGTGGTGGTCACAGTGCTCAACCTGC
TGGTTGCGATATTGACCGTATCGCACTCCAAGGTACAGGAGAACGTTCAA
CGGGAGTTCAAGGTTTCGAAGGCGCGCATGATCCAGCGATGCCGATGGAC
TGTAGAAAACGACTGGCTTCCTGCCCCGTTCAACGTGGCACAGCTACTTT
TCTCGGGGCAATACGCGTTGGTGATGCCCAGCTTTCGGCGGAAACGGTAT
GATGAAGCAAAGCAAGCCATTGGTCAGGCAGTGTCCTGGTTTGTGCTCGG
TCTAATGGCTTTTGTGGGGGGATCGATTCTCTGGATATTGTCGGTTGTGT
ACGCACTCTTTGTGTGGCATGAGCACTACTACGTAGGGCTAGACACGCAT
ACTGCTCACGTTATAATACTTCAAACTGAGCCTGGGAGCAACGATACTGC
GGTATATCCTTATCCCTGTGTGGTGCGTCTTGGATGCTCCGTTGTGTCGT
TTCCTGCTCGGGGTCAAGACGCTTCTACTCGGGCAAGTAGCGGGGGAGGT
ACACCCTGCCGAACCGGGTAGTCTATACCGGTAACAGTAGATGGCATGCT
TAAGAGTGCCGATCAGGGAGTAGGAATGGCAAAATTGCGCGAATTCCTCG
CCGACCCAATGAACAACAGAGATGTTCGTAAGGCCGGGACGACAAGGAGC
ACCACCGTGGAACACCTTAAACTTCTTAGGGACCGCCTAGTGAAAGACAT
TGTAGGTAGGCTCTGCAGACTTTTGAGAAAAGGGAAGGCATGGCCCAAAC
ACTTGATTGAGGCTTTCGATCAGCGCTTGCAACAACTCGAAGCAAAGGTT
GCCGAGACCAATGATTACCTAATTGAAATTTTGAAGTTCGTCAAGAACCC
ACGGTGA

Coding sequence (CDS) from alignment at Scaffold_195:652100..656506+

>EsuBft1254_7a-0001 ID=EsuBft1254_7a-0001|Name=EsuBft1254_7a-0001|organism=Ectocarpus subulatus male Bft15b|type=CDS|length=1950bp|location=Sequence derived from alignment at Scaffold_195:652100..656506+ (Ectocarpus subulatus male Bft15b)
ATGACCAGCGCTCAATTTTCAATATGGGCTAGTGCTGGACTTGGCAAGTG
GGACGGTATCGCACTGCTTCTGAAGAATTATGCATGTCCGTCTCCTGAAG
ACCTCATTGATCTCTCTCGCAAGCACCCTTCTGACGAGCGGCTGCACGAC
TGCTGTTTGAGAGTTGTAGCCGCGTCAGACAACCTGTTCATTTCCGGCAT
GACTTTGTCTGTGGCACGCGTGGATGTGACGAAGAAAGCTCCAGAGGGAG
AGAGGGGTACCCTTGAGGCCATCCAGGGCCATGTACATGCCTTACTGCTT
GAAATTCTCGAACGCTTGCCTCAAACAGTCAATGGTTTCAACGGAGGAAT
GGCCGACTGCTCAGCCGTGTTAGGGCCAGAGTTCGACAGCAAAACGGGAG
AATCTACGGGCTTCAAGCCACCGCTGGCCCTGCTCCTCCTGGCGAACAGT
ATTCAGACGGAAACGTTTTGCTCGACGCCCTTAATCATGGACTACTTGTC
ACGGAAATTCACGCATGGTCTGCCCGACGTGGGCGACAAGGACGGCGTGC
TTCGGAATGGCACAGAACATGATGTGCTGAGCGGCAGTGGGCCAGGCATG
ATGTCCCTGGTCATTGGTGGTGGCGATGACGAAGAACTGGACGAAAGAGG
TTATCACGAATTCCGACACAAGGATCTTCTGGTCAAGAATGGCCTAAAGG
CCAGCTTTGAATCCCCTCGTGCGTTGCTTCAGGGAGCGAATGATAGGCTT
GCCAGCCTGACGTTTCTTCCTGGGGCTCAATTTGCGATCGCAGGATTTCT
GGCGAAGCCTAACAACTACTATCGAATCCCCGCTGTGAGGATGGTGCTAG
ACGTTGTGGTGTACCTCTTGGTGATCATTGCGTTGAGCATCCTGGTCTTC
CGCGACAGCGGGTCGCTGACACGGGGAGAGGGCATCGCCGCGGCTGTTAT
TTTCGCGATGACGGCTCAGCTCGTGAGGTTCGGGATTGTGATGTTGGTTG
TCATGGTCGGATTCGCCATGTCCCTGCACGTGCTGATTCGCGACTCCAAA
AGCTTTGGTGAAACGTTTGTCGCACTATTCAAAGCGACGCTTGGTGACGT
GGACTACTTCACGGCGTCCTCTGTGCTCAACCTGCTGGTTGCGATATTGA
CCGTATCGCACTCCAAGGTACAGGAGAACGTTCAACGGGAGTTCAAGGTT
TCGAAGGCGCGCATGATCCAGCGATGCCGATGGACTGTAGAAAACGACTG
GCTTCCTGCCCCGTTCAACGTGGCACAGCTACTTTTCTCGGGGCAATACG
CGTTGGTGATGCCCAGCTTTCGGCGGAAACGGTATGATGAAGCAAAGCAA
GCCATTGGTCAGGCAGTGTCCTGGTTTGTGCTCGGTCTAATGGCTTTTGT
GGGGGGATCGATTCTCTGGATATTGTCGGTTGTGTACGCACTCTTTGTGT
GGCATGAGCACTACTACGTAGGGCTAGACACGCATACTGCTCACGTTATA
ATACTTCAAACTGAGCCTGGGAGCAACGATACTGCGGTATATCCTTATCC
CTGTGTGGTGCGTCTTGGATGCTCCGTTGTGTCGTTTCCTGCTCGGGGTC
AAGACGCTTCTACTCGGGCAAGTAGCGGGGGAGATGGCATGCTTAAGAGT
GCCGATCAGGGAGTAGGAATGGCAAAATTGCGCGAATTCCTCGCCGACCC
AATGAACAACAGAGATGTTCGTAAGGCCGGGACGACAAGGAGCACCACCG
TGGAACACCTTAAACTTCTTAGGGACCGCCTAGTGAAAGACATTGTAGGT
AGGCTCTGCAGACTTTTGAGAAAAGGGAAGGCATGGCCCAAACACTTGAT
TGAGGCTTTCGATCAGCGCTTGCAACAACTCGAAGCAAAGGTTGCCGAGA
CCAATGATTACCTAATTGAAATTTTGAAGTTCGTCAAGAACCCACGGTGA