EsuBft1813_3 (polypeptide) Ectocarpus subulatus male Bft15b

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NameEsuBft1813_3a-0001
Unique NameEsuBft1813_3
Typepolypeptide
OrganismEctocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)
Sequence length727
Homology
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 1487 bits (3849), Expect = 0.000e+0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 0
Query:    1 MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727
            MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW
Sbjct:    1 MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1105 bits (2858), Expect = 0.000e+0
Identity = 578/753 (76.76%), Postives = 605/753 (80.35%), Query Frame = 0
Query:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEG------------------------------------------VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
            V ALPSSATAVIRAPEDFDLLS  PLL SLKLATSLLDHGPDTSPRLGAAD+ DLSDHSAPLRSTYGEG                                          VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGM +LLPGKRFPSETRTACYMGLLSQLAAKPEVL VSPLQSA LHNAVANA +QSAT       P    G           DSGLD+TSCFFADDDGLQVEHG+LFDGVRVEDDGNVST LGNYSFPFDMSRRK                                                 IVQ IQLFKTP QGSTSSGQDSFT+ SD++GDP+DYDDSEYISY DYSGEYYSDTWSEDSFCAG LDDLSYH SYL+DDTSY+S STSPSFTGGFH+DT+GGHGT T+GSAAGAIS+QS L+EEACYGDELPGCAGGCISASDVDAMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGGVAPGAQISVFD SYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YDTFMYENPEHLLIF+AGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNL  EE+G+TDYSPEGYPWICL P LG P ASTEPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSCKL
Sbjct:   99 VTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSAT---TTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCKL 798          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 936 bits (2420), Expect = 0.000e+0
Identity = 502/717 (70.01%), Postives = 550/717 (76.71%), Query Frame = 0
Query:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFI----GDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYA--SPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
            V ALPSSATA+I APEDF LLS  P L SLKLAT LLDHGPDTSP +GAAD+ +LSD SAPLRSTYGE               DRS+YDVTRVREWSRAAAVVD LASK A+T GEICGFGKL LRHVADDL+LVEGM +LLPGK+ PSE RTACYMGLLSQLAAKPEVL VSPLQSA+L+NAVANA +QSAT       P    G           D+GLD+TSCFFADDDGLQVEHG+LF GVRVE DGNVST LGNYSFPFDMSRRK                                                 IVQ IQ+FKTP QGSTSSGQ+SFT+ SD+I    GD FD DDSEY SY DYSGEY SDTWSE+SFCA  LDDLSYH S L+DDTSY+  S S+S S TGGF +D +GGHGT T+G AAGAIS+QSSL+EEACYGDELPGCAGGCISASDVDAMLD+ +F L VFCPMY CDGN DIPSSECLSDDPVE+LHQHGGVAPGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG   LCQLTE EY+YD+FMYENPEHLLIF+AGN GG +DIPN E CT+GSPALGKNILAVGATSSGP  GTRTG+DGNL  EE G+TDYSPEGYPWICL P LG P AS EPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSC+L
Sbjct:  109 VTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE---------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSAT---TTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCRL 757          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 699 bits (1803), Expect = 2.930e-245
Identity = 358/484 (73.97%), Postives = 374/484 (77.27%), Query Frame = 0
Query:  328 KTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727
            KTP QGSTSSGQDSFT+FSD+ GDP+DYDDSEYIS+ DYSGEY+SDT                                                 W                                   SE+    GGLDDL+YHLSYLYDDTSYAS STSPSFTGGFH+DTIGGHGT T+GSAAGAISSQSSL+EEACYGDELP CAGGCISASDV+AMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGG APGAQISVFD SYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYD FMYENPEHLLIFSAGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNLF EEVG+TDYSPEGYPWICLMP LG P ASTEPAGVDTV YFSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKLAGPP PAPW PVPLPW
Sbjct:   37 KTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 286 bits (731), Expect = 2.710e-80
Identity = 247/760 (32.50%), Postives = 336/760 (44.21%), Query Frame = 0
Query:   10 SSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDH-------GPDTS--PRLGAAD------------NLDLSDHSAPLRSTYGEG-------------VNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFP--------SETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVV-----FCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYA--SWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
            + A+A++   E F L S  P  S+LKLA  L+DH       G  +S   RLGA              N+ L+  + P RS   E              V++   +FWSD      E+        R R+WS+AA+VV  L+  +  + G+IC + ++ L    DD +LV G+ +LL   R          +E   AC+MGL+S  A +PEVL VSP  +  + N  A   +QSA+  D    P    G           D+GLD+TSCFF DD G +V+HGY F+ + V   G  S+ L  Y                     +   GD T     T+ R                    +++Q I+L                              DS+Y+++    G  Y                      +L  D               F QD   GHGT T+GSAAGA  +  + +   C GDE  GC GGCI  S+    L      + +      CPMY CDG+ D     CLSD+  E L ++GG+A GA++S+FD  +   EV+      GN +W   +E G K+H+ S GG TLC   E E +YD FMYENPE+LLIF+AGNDG  +    R  CT+ SPA+GKN+LAVGA++SG         D  +   E G                           AG+D V  FSS G T DG+IKP VVAPG+ V  SA+SDGTD HSC+L
Sbjct:  123 AQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATD---TPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTG--SSQLIYYP--------------------VFEAGDFT----YTEERR-------------------KVIQYIELVNP---------------------------DSDYVAFSTTQGGVYP---------------------FLPPDE--------------FGQDDSAGHGTHTAGSAAGATLNDPA-ETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVY-SAASDGTDSHSCRL 723          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 255 bits (652), Expect = 7.600e-69
Identity = 209/647 (32.30%), Postives = 295/647 (45.60%), Query Frame = 0
Query:   97 VREWSRAAAVVDGLA-SKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLP------GKRFPS--ETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQ-VEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKE-----EACYGDELPGCAGGCISASDVDAMLDDST-------------FHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
             REW+RAA V+ GL  ++   T+G++C +  L + H  DDL+ ++GM +LLP      G   P   E + AC+M LLS LA++PEVL VSP ++  L NA A  ++QSA+  +   +P    G           D+GLD  SCFF D+DG + V HGY F+ + VE     +++ G++   +D + +          +  + TG                   F+F      G++             H    S                     S   + GD         WS ++      +  +Y  S+     + A           F +D  GGHGT T+GSAAGA+ S  S  +     E C       C GGCI   D     D  T               L   CP   CD   D+P   CL DD  E L QHGGVA GAQ+++FD     T V    AGN +W  AME G+K+ +NSWG    C     + ++D FMY N + LL+F+AGNDG  DD      CT+G+PAL KN+LAVGA++SGP   + T +DG+    +  V D                          +D + YFSS GPTLD +IKP VVAPG+ + ++A S G   ++C+L
Sbjct:  307 AREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTE---SPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVE-----ASSFGSWFTSWDFNVQ----------IARVFTG-----------------GDFNFDLSRRKGLVVDAA---------HPSCDSPNI------------------SRRFALGDVRA------WSGENPACTANNSTNYGESWA----TSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDG--DDW---SGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGS-GDGNYTCRL 846          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 254 bits (648), Expect = 1.610e-68
Identity = 234/759 (30.83%), Postives = 328/759 (43.21%), Query Frame = 0
Query:   10 SSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDL--SDHSAPLRSTYGEG--------------------------------VNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFP---------SETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP-DSGLDKTSCFFA-DDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGA-ISSQSSLKEEACYGDELPGCAGGCIS---ASDVDAMLDDST--FHLVVFCPMYTCDGNIDIPSSE-CLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
            + A+A+I   E F L S  P  S+LKLA  L+DH             LD   + H A +R +  EG                                V++   +FWSD +    E         R R+WS+AA+VV  L+  +  + G+IC + ++ + H   D++LV G+ +LL   R           +E   AC+MGL+S LA+KPEVL V+P QS  L NA A A++QSAT  D         G        DSG+D++SCFFA D+ G  V HG+ F+   +    N      ++S P       +G   A        T D  T        E      FD   F+      +I+Q I L K+    +  S  DSFT                                            L     +L  D               F  D   GHGT  +GS AGA +++ + L    C   E   C G C+    A+DVD +   +     L   CP+++ D + D+  +E CLSDD  E L  +GG+A GA++++FD  Y    + + + GN LW    E   KIH+NS+GG   C L   + +YD +M  NPE+LLIF+AGNDG       R  CT+ SPA+ KN+LAVGA++SG    TR                              L A P      G+DTV  FSS GPT D +IKP V+APG+ + ++AS    D HSC+L
Sbjct:  113 AQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGI----NPPLTSSSFSSPTSDDDDSLGWPRA--------TDDHRTGSYYQIAEE------FDGGDFTIFPDRRKIIQYINLIKS--DDTEGSHGDSFT------------------------------------------TSLGQQYPWLPADR--------------FEYDYEAGHGTHVAGSVAGATLNTPAELIS--CKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGER----RRSTCTMSSPAIAKNVLAVGASTSGD---TR------------------------------LSATPG----LGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRL 750          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 241 bits (616), Expect = 1.970e-64
Identity = 205/663 (30.92%), Postives = 282/663 (42.53%), Query Frame = 0
Query:   66 LRSTYGEGVNMRRASFWSD-----SDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPS--------ETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
            L+S     +++   + WSD      D   S     R REWSRAA +V+  +     T  ++CG+ ++ + H A+D++LV G+ +LL   R  S        E R ACYMGL+S LA++ EVL V+        NA A A++QSA+    ++ P    G           D+G+++T+CF    DG +VEHGY FD V          A  N  F      R + K                                F    FS+     +++Q I L   P  G  SS    F     F+ D                                  DDL                                GHGT  + + AG+  S  +       G E+ GCAGGCI+ S   +   D    +   CP + C G+      +CL DD  + L +HGG+A GA+I+  D  Y G   Y   AGN LW +  + G KIH+NS+G    CQLT  + +YD +MYEN EHLL+FSAGN G   D   R+ CT+ SP + KN+L VG+TSSG    TR           +  TD    GY                E + +D V  FS  G T DG+IKP VVAPG+ +  SAS +G D  SC+L
Sbjct:  208 LKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASV---DSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFV----------ASSNPLFTSSSESRSMFKAE------------------------------FTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD----------------------------------DDL--------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGV---TR-----------LSTTDLDGRGYA------------DGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIY-SASGNGGDS-SCEL 722          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 201 bits (512), Expect = 2.920e-52
Identity = 190/639 (29.73%), Postives = 275/639 (43.04%), Query Frame = 0
Query:   97 VREWSRAAAVVDGLA-SKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANAS--MQSATYHDRNAAPRCWIGRHWRSHP--DSGLDKTS-CFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKE-----EACYGDELPGCAGGCISASDV---DAMLDDSTFH----------LVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711
             REW+RAA V+ GL  ++   T+G++C +  L + H  DDL+ ++GM +LLP  R            L  Q+A    +L     +   L  + A A   + +A YH+  +A       +    P  D+GLD T       D GL V   +      V++DG    A G+Y                 E +G+  +   +         +I  +  F    F+      +IVQ I L +      T+S  ++ T + +              S+   +G+ Y   W                              ++P F   +     GGHGT T+GSAAGA+ S  S  +     E C       C GGCI   D    D ++    +           L   CP   CD   D P   CL DD  E L QHGGVA GAQ+++FD     T +    AGN +W  AME G+K+ +NSWG    C+    + +YD FMY N + LL+F+AGNDG          CT+GSPA+ KN+LAVGA++SGP   + T +DG+    +  V D                          +D + YFSS GPTLDG+IKP VVAPG+ +  SA+  G   ++C+L
Sbjct:   43 AREWTRAADVLRGLGGAEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADEL--QVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSA-------NLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFF-----VDEDGGEGVAHGHYF----------------EEIGVEASALSSWFTSWDFNLQIARV--FTGGDFNFDLSRRKIVQYINLIR--QDSETNSTANNSTNYGE--------------SWATSAGDQYP--WI-----------------------------SAPEFAENY----AGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMWEPAMEAGSKLSSNSWGIYAYCEYLSYDVLYDDFMYRNQDQLLLFAAGNDG-----EEWSGCTMGSPAVAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDGRIKPEVVAPGDMIY-SAAGGGDGNYTCRL 563          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 203 bits (516), Expect = 1.720e-51
Identity = 127/306 (41.50%), Postives = 166/306 (54.25%), Query Frame = 0
Query:  416 QDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISAS------DVDAMLDD-STFHLVV--FCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLA 712
            +D + GHGT  +GSA GA     +     C G E+PGC GGCI A       D+  ++ + S + + +   CPM  CD   D     CLSDD  + L  HGG+A GA+++ FD    G+     + GN LW   +E G KIH+ S G    C + EK+ +YD FMY NPE+L++ +AGN G  +D   R  CT+ SPA+GKN LAVGATSSG    T+T  DG+L     G  D                          VDTV  FSS GPT DG+IKP VVAPG+ +  SA SDG+D HSC+L+
Sbjct:  413 RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCD---DATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSG-THFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIY-SARSDGSDNHSCRLS 685          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 1489 bits (3854), Expect = 0.000e+0
Identity = 727/727 (100.00%), Postives = 727/727 (100.00%), Query Frame = 1
Query:    1 MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 2181
            MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW
Sbjct:    1 MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 727          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1105 bits (2858), Expect = 0.000e+0
Identity = 578/753 (76.76%), Postives = 605/753 (80.35%), Query Frame = 1
Query:   13 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEG------------------------------------------VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
            V ALPSSATAVIRAPEDFDLLS  PLL SLKLATSLLDHGPDTSPRLGAAD+ DLSDHSAPLRSTYGEG                                          VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGM +LLPGKRFPSETRTACYMGLLSQLAAKPEVL VSPLQSA LHNAVANA +QSAT       P    G           DSGLD+TSCFFADDDGLQVEHG+LFDGVRVEDDGNVST LGNYSFPFDMSRRK                                                 IVQ IQLFKTP QGSTSSGQDSFT+ SD++GDP+DYDDSEYISY DYSGEYYSDTWSEDSFCAG LDDLSYH SYL+DDTSY+S STSPSFTGGFH+DT+GGHGT T+GSAAGAIS+QS L+EEACYGDELPGCAGGCISASDVDAMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGGVAPGAQISVFD SYVGTE+YAS+AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YDTFMYENPEHLLIF+AGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNL  EE+G+TDYSPEGYPWICL P LG P ASTEPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSCKL
Sbjct:   99 VTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSAT---TTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCKL 798          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 936 bits (2420), Expect = 0.000e+0
Identity = 502/717 (70.01%), Postives = 550/717 (76.71%), Query Frame = 1
Query:   13 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFI----GDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYA--SPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
            V ALPSSATA+I APEDF LLS  P L SLKLAT LLDHGPDTSP +GAAD+ +LSD SAPLRSTYGE               DRS+YDVTRVREWSRAAAVVD LASK A+T GEICGFGKL LRHVADDL+LVEGM +LLPGK+ PSE RTACYMGLLSQLAAKPEVL VSPLQSA+L+NAVANA +QSAT       P    G           D+GLD+TSCFFADDDGLQVEHG+LF GVRVE DGNVST LGNYSFPFDMSRRK                                                 IVQ IQ+FKTP QGSTSSGQ+SFT+ SD+I    GD FD DDSEY SY DYSGEY SDTWSE+SFCA  LDDLSYH S L+DDTSY+  S S+S S TGGF +D +GGHGT T+G AAGAIS+QSSL+EEACYGDELPGCAGGCISASDVDAMLD+ +F L VFCPMY CDGN DIPSSECLSDDPVE+LHQHGGVAPGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG   LCQLTE EY+YD+FMYENPEHLLIF+AGN GG +DIPN E CT+GSPALGKNILAVGATSSGP  GTRTG+DGNL  EE G+TDYSPEGYPWICL P LG P AS EPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSC+L
Sbjct:  109 VTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE---------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSAT---TTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCRL 757          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 701 bits (1808), Expect = 5.780e-245
Identity = 358/484 (73.97%), Postives = 374/484 (77.27%), Query Frame = 1
Query:  982 KTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLAGPPFPAPWSPVPLPW 2181
            KTP QGSTSSGQDSFT+FSD+ GDP+DYDDSEYIS+ DYSGEY+SDT                                                 W                                   SE+    GGLDDL+YHLSYLYDDTSYAS STSPSFTGGFH+DTIGGHGT T+GSAAGAISSQSSL+EEACYGDELP CAGGCISASDV+AMLDDSTF L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGG APGAQISVFD SYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYD FMYENPEHLLIFSAGNDGGY+DIPNREACTVGSPALGKN LAVGATSSGPFPGTRTGDDGNLF EEVG+TDYSPEGYPWICLMP LG P ASTEPAGVDTV YFSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKLAGPP PAPW PVPLPW
Sbjct:   37 KTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKLAGPPLPAPWLPVPLPW 519          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 286 bits (731), Expect = 1.190e-79
Identity = 247/760 (32.50%), Postives = 336/760 (44.21%), Query Frame = 1
Query:   28 SSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDH-------GPDTS--PRLGAAD------------NLDLSDHSAPLRSTYGEG-------------VNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFP--------SETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVV-----FCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYA--SWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
            + A+A++   E F L S  P  S+LKLA  L+DH       G  +S   RLGA              N+ L+  + P RS   E              V++   +FWSD      E+        R R+WS+AA+VV  L+  +  + G+IC + ++ L    DD +LV G+ +LL   R          +E   AC+MGL+S  A +PEVL VSP  +  + N  A   +QSA+  D    P    G           D+GLD+TSCFF DD G +V+HGY F+ + V   G  S+ L  Y                     +   GD T     T+ R                    +++Q I+L                              DS+Y+++    G  Y                      +L  D               F QD   GHGT T+GSAAGA  +  + +   C GDE  GC GGCI  S+    L      + +      CPMY CDG+ D     CLSD+  E L ++GG+A GA++S+FD  +   EV+      GN +W   +E G K+H+ S GG TLC   E E +YD FMYENPE+LLIF+AGNDG  +    R  CT+ SPA+GKN+LAVGA++SG         D  +   E G                           AG+D V  FSS G T DG+IKP VVAPG+ V  SA+SDGTD HSC+L
Sbjct:  123 AQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATD---TPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTG--SSQLIYYP--------------------VFEAGDFT----YTEERR-------------------KVIQYIELVNP---------------------------DSDYVAFSTTQGGVYP---------------------FLPPDE--------------FGQDDSAGHGTHTAGSAAGATLNDPA-ETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVY-SAASDGTDSHSCRL 723          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 255 bits (652), Expect = 2.800e-68
Identity = 209/647 (32.30%), Postives = 295/647 (45.60%), Query Frame = 1
Query:  289 VREWSRAAAVVDGLA-SKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLP------GKRFPS--ETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQ-VEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKE-----EACYGDELPGCAGGCISASDVDAMLDDST-------------FHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
             REW+RAA V+ GL  ++   T+G++C +  L + H  DDL+ ++GM +LLP      G   P   E + AC+M LLS LA++PEVL VSP ++  L NA A  ++QSA+  +   +P    G           D+GLD  SCFF D+DG + V HGY F+ + VE     +++ G++   +D + +          +  + TG                   F+F      G++             H    S                     S   + GD         WS ++      +  +Y  S+     + A           F +D  GGHGT T+GSAAGA+ S  S  +     E C       C GGCI   D     D  T               L   CP   CD   D+P   CL DD  E L QHGGVA GAQ+++FD     T V    AGN +W  AME G+K+ +NSWG    C     + ++D FMY N + LL+F+AGNDG  DD      CT+G+PAL KN+LAVGA++SGP   + T +DG+    +  V D                          +D + YFSS GPTLD +IKP VVAPG+ + ++A S G   ++C+L
Sbjct:  307 AREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTE---SPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVE-----ASSFGSWFTSWDFNVQ----------IARVFTG-----------------GDFNFDLSRRKGLVVDAA---------HPSCDSPNI------------------SRRFALGDVRA------WSGENPACTANNSTNYGESWA----TSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDG--DDW---SGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGS-GDGNYTCRL 846          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 254 bits (648), Expect = 5.830e-68
Identity = 234/759 (30.83%), Postives = 328/759 (43.21%), Query Frame = 1
Query:   28 SSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDL--SDHSAPLRSTYGEG--------------------------------VNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFP---------SETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP-DSGLDKTSCFFA-DDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGA-ISSQSSLKEEACYGDELPGCAGGCIS---ASDVDAMLDDST--FHLVVFCPMYTCDGNIDIPSSE-CLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
            + A+A+I   E F L S  P  S+LKLA  L+DH             LD   + H A +R +  EG                                V++   +FWSD +    E         R R+WS+AA+VV  L+  +  + G+IC + ++ + H   D++LV G+ +LL   R           +E   AC+MGL+S LA+KPEVL V+P QS  L NA A A++QSAT  D         G        DSG+D++SCFFA D+ G  V HG+ F+   +    N      ++S P       +G   A        T D  T        E      FD   F+      +I+Q I L K+    +  S  DSFT                                            L     +L  D               F  D   GHGT  +GS AGA +++ + L    C   E   C G C+    A+DVD +   +     L   CP+++ D + D+  +E CLSDD  E L  +GG+A GA++++FD  Y    + + + GN LW    E   KIH+NS+GG   C L   + +YD +M  NPE+LLIF+AGNDG       R  CT+ SPA+ KN+LAVGA++SG    TR                              L A P      G+DTV  FSS GPT D +IKP V+APG+ + ++AS    D HSC+L
Sbjct:  113 AQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGI----NPPLTSSSFSSPTSDDDDSLGWPRA--------TDDHRTGSYYQIAEE------FDGGDFTIFPDRRKIIQYINLIKS--DDTEGSHGDSFT------------------------------------------TSLGQQYPWLPADR--------------FEYDYEAGHGTHVAGSVAGATLNTPAELIS--CKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGL-SPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGER----RRSTCTMSSPAIAKNVLAVGASTSGD---TR------------------------------LSATPG----LGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRL 750          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 241 bits (616), Expect = 6.550e-64
Identity = 205/663 (30.92%), Postives = 282/663 (42.53%), Query Frame = 1
Query:  196 LRSTYGEGVNMRRASFWSD-----SDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPS--------ETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
            L+S     +++   + WSD      D   S     R REWSRAA +V+  +     T  ++CG+ ++ + H A+D++LV G+ +LL   R  S        E R ACYMGL+S LA++ EVL V+        NA A A++QSA+    ++ P    G           D+G+++T+CF    DG +VEHGY FD V          A  N  F      R + K                                F    FS+     +++Q I L   P  G  SS    F     F+ D                                  DDL                                GHGT  + + AG+  S  +       G E+ GCAGGCI+ S   +   D    +   CP + C G+      +CL DD  + L +HGG+A GA+I+  D  Y G   Y   AGN LW +  + G KIH+NS+G    CQLT  + +YD +MYEN EHLL+FSAGN G   D   R+ CT+ SP + KN+L VG+TSSG    TR           +  TD    GY                E + +D V  FS  G T DG+IKP VVAPG+ +  SAS +G D  SC+L
Sbjct:  208 LKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASV---DSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFV----------ASSNPLFTSSSESRSMFKAE------------------------------FTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD----------------------------------DDL--------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGV---TR-----------LSTTDLDGRGYA------------DGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIY-SASGNGGDS-SCEL 722          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: D8LBZ2_ECTSI (Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBZ2_ECTSI)

HSP 1 Score: 201 bits (512), Expect = 6.890e-52
Identity = 190/639 (29.73%), Postives = 275/639 (43.04%), Query Frame = 1
Query:  289 VREWSRAAAVVDGLA-SKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANAS--MQSATYHDRNAAPRCWIGRHWRSHP--DSGLDKTS-CFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKE-----EACYGDELPGCAGGCISASDV---DAMLDDSTFH----------LVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 2133
             REW+RAA V+ GL  ++   T+G++C +  L + H  DDL+ ++GM +LLP  R            L  Q+A    +L     +   L  + A A   + +A YH+  +A       +    P  D+GLD T       D GL V   +      V++DG    A G+Y                 E +G+  +   +         +I  +  F    F+      +IVQ I L +      T+S  ++ T + +              S+   +G+ Y   W                              ++P F   +     GGHGT T+GSAAGA+ S  S  +     E C       C GGCI   D    D ++    +           L   CP   CD   D P   CL DD  E L QHGGVA GAQ+++FD     T +    AGN +W  AME G+K+ +NSWG    C+    + +YD FMY N + LL+F+AGNDG          CT+GSPA+ KN+LAVGA++SGP   + T +DG+    +  V D                          +D + YFSS GPTLDG+IKP VVAPG+ +  SA+  G   ++C+L
Sbjct:   43 AREWTRAADVLRGLGGAEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGNEGPEADEL--QVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSA-------NLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFF-----VDEDGGEGVAHGHYF----------------EEIGVEASALSSWFTSWDFNLQIARV--FTGGDFNFDLSRRKIVQYINLIR--QDSETNSTANNSTNYGE--------------SWATSAGDQYP--WI-----------------------------SAPEFAENY----AGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDTFGGDDLITTWDYQYFWAGYEAIDLDRLCPRLDCD---DRPEEYCLGDDEAETLQQHGGVARGAQLAIFDILDDYTGIGIEMAGNGMWEPAMEAGSKLSSNSWGIYAYCEYLSYDVLYDDFMYRNQDQLLLFAAGNDG-----EEWSGCTMGSPAVAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQD--------------------------IDAMAYFSSFGPTLDGRIKPEVVAPGDMIY-SAAGGGDGNYTCRL 563          
BLAST of EsuBft1813_3a-0001 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 203 bits (516), Expect = 4.190e-51
Identity = 127/306 (41.50%), Postives = 166/306 (54.25%), Query Frame = 1
Query: 1246 QDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISAS------DVDAMLDD-STFHLVV--FCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKLA 2136
            +D + GHGT  +GSA GA     +     C G E+PGC GGCI A       D+  ++ + S + + +   CPM  CD   D     CLSDD  + L  HGG+A GA+++ FD    G+     + GN LW   +E G KIH+ S G    C + EK+ +YD FMY NPE+L++ +AGN G  +D   R  CT+ SPA+GKN LAVGATSSG    T+T  DG+L     G  D                          VDTV  FSS GPT DG+IKP VVAPG+ +  SA SDG+D HSC+L+
Sbjct:  413 RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCD---DATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSG-THFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIY-SARSDGSDNHSCRLS 685          
The following BLAST results are available for this feature:
BLAST of EsuBft1813_3a-0001 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JQ52_9PHAE0.000e+0100.00Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LSW0_ECTSI0.000e+076.76Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LU20_ECTSI0.000e+070.01Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5KDF8_9PHAE2.930e-24573.97Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5K229_9PHAE2.710e-8032.50Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A... [more]
A0A6H5JKA8_9PHAE7.600e-6932.30Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5JQT4_9PHAE1.610e-6830.83Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LQI1_ECTSI1.970e-6430.92Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LBZ2_ECTSI2.920e-5229.73Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculos... [more]
A0A2I6U1R2_SACJA1.720e-5141.50Subtilisin (Fragment) n=1 Tax=Saccharina japonica ... [more]

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BLAST of EsuBft1813_3a-0001 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JQ52_9PHAE0.000e+0100.00Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LSW0_ECTSI0.000e+076.76Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LU20_ECTSI0.000e+070.01Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A6H5KDF8_9PHAE5.780e-24573.97Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5K229_9PHAE1.190e-7932.50Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A... [more]
A0A6H5JKA8_9PHAE2.800e-6832.30Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
A0A6H5JQT4_9PHAE5.830e-6830.83Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 Tax... [more]
D8LQI1_ECTSI6.550e-6430.92Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
D8LBZ2_ECTSI6.890e-5229.73Subtilisin (Fragment) n=1 Tax=Ectocarpus siliculos... [more]
A0A2I6U1R2_SACJA4.190e-5141.50Subtilisin (Fragment) n=1 Tax=Saccharina japonica ... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 505..699
e-value: 1.9E-19
score: 70.0
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 372..715
e-value: 9.2E-25
score: 89.4
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 278..702
NoneNo IPR availablePANTHERPTHR43399:SF1PROPROTEIN CONVERTASE, PUTATIVE-RELATEDcoord: 393..617
coord: 657..706
NoneNo IPR availablePANTHERPTHR43399SUBTILISIN-RELATEDcoord: 393..617
coord: 657..706

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Scaffold_647contigScaffold_647:167760..177647 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Diamond blastp: OGS1.0 vs UniRef902022-09-16
OGS1.0 of Ectocarpus subulatus male Bft15b2020-06-19
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
EsuBft1813_3a-0001EsuBft1813_3a-0001Ectocarpus subulatus male Bft15bmRNAScaffold_647 167760..177852 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>EsuBft1813_3 ID=EsuBft1813_3|Name=EsuBft1813_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=727bp
MVERVAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPR
LGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREW
SRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKR
FPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHD
RNAAPRCWIGRHWRSHPDSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDD
GNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREI
LEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIG
DPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTS
YASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDEL
PGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPV
EDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTN
SWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTV
GSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWIC
LMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASAS
SDGTDEHSCKLAGPPFPAPWSPVPLPW
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR000209Peptidase_S8/S53_dom
IPR036852Peptidase_S8/S53_dom_sf