EsuBft1017_6 (polypeptide) Ectocarpus subulatus male Bft15b

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NameEsuBft1017_6a-0001
Unique NameEsuBft1017_6
Typepolypeptide
OrganismEctocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)
Sequence length842
Homology
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A6H5JBC7_9PHAE (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBC7_9PHAE)

HSP 1 Score: 1593 bits (4126), Expect = 0.000e+0
Identity = 842/842 (100.00%), Postives = 842/842 (100.00%), Query Frame = 0
Query:    1 MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 842
            MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW
Sbjct:    1 MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 842          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: D7G5M6_ECTSI (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5M6_ECTSI)

HSP 1 Score: 1183 bits (3060), Expect = 0.000e+0
Identity = 619/663 (93.36%), Postives = 622/663 (93.82%), Query Frame = 0
Query:  180 MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 842
            MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLT+DKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIK                                    VNYPSACNAAETILIHKALLEEGAGTC+QVVRALHS GVTVLGGPRAVDLGVVGGDKISPGFKTEYG  DLTVSLEIVDSVEAALDHIQ FGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGE+AHTVGKKDPY LKPMDLEAGAW
Sbjct:    1 MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTKDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIK------------------------------------VNYPSACNAAETILIHKALLEEGAGTCSQVVRALHSAGVTVLGGPRAVDLGVVGGDKISPGFKTEYG--DLTVSLEIVDSVEAALDHIQRFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGETAHTVGKKDPYSLKPMDLEAGAW 625          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A835ZBW4_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBW4_9STRA)

HSP 1 Score: 884 bits (2283), Expect = 3.010e-312
Identity = 477/728 (65.52%), Postives = 565/728 (77.61%), Query Frame = 0
Query:  107 SRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGR-------RNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLY--EDSDDEEDADDMIVR-QAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV 824
            SR  ++ A RIV+KAGTS+VS+ RGYP L+R+GA+VEQCAWL+KMGKEV+LVSSGAVGVGRQLLRKQQLL++S+G VLDGH     G G         +Y+SACAAAGQLGLMSLY+TLF+QCD ATSQ+L+T  DF TPER R+LRYT +TML+LGV+PI+NENDAVS N+GY  DG FSDNDGLAA+VA+QM A++L+LLTDV+GVY+K P   GA+I HT+ P   E+ IG+KS GGRGGM AKI AA RAV  GV SVVIA+G NP+SIE++ CGE+VGT+FC N       D  +E++A    VR  AE AR GGRALC L +EER   L  VA+AL A+  E+  AN RD++ A+   +A  LL RL+LT  KI+ L +GI SLAD  EP+ KC S LEV EGLVLKQ+T+PIGVLL+VFESRPDSLPQIASLA+RSGNGLLLKGGKEAEHSNACLH II DAV E + GRV R+ IGLV  R AV ELLKLD LIDLVIPRGSG LVN IK++TKIPV+GH+EG+CH++ID AAD  KA+ +AVDAK+NYP+ACNA ET+L+H+  +    G   +V  AL S G  VLGGPRA+  G+    + +P FKTEYGE  L  SLE+VD   AA+DHI  FGS HTE IVTED   A+ FLE VDSACVFHNASTRFSDG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKWVLRS    AHTV
Sbjct:   38 SRQKVLHAHRIVIKAGTSVVSDERGYPSLTRLGAIVEQCAWLRKMGKEVLLVSSGAVGVGRQLLRKQQLLNSSLGQVLDGH----DGNGAALLQAKSNSYNSACAAAGQLGLMSLYDTLFSQCDTATSQMLLTADDFGTPERCRNLRYTMNTMLQLGVIPIVNENDAVSANQGYTKDGTFSDNDGLAAVVAQQMGAQLLVLLTDVDGVYDKAPTLPGARIIHTYLPAT-EVQIGDKSVGGRGGMSAKISAAMRAVEAGVASVVIANGCNPFSIEQVCCGEKVGTLFCQNAAAMAARDLPEEDEALTEKVRVAAEAARAGGRALCQLSTEERTQALHAVAKALGARQAEVLAANARDMQEAERVKLAGPLLKRLQLTPAKIQTLVDGINSLADQPEPIGKCMSHLEVSEGLVLKQVTTPIGVLLVVFESRPDSLPQIASLALRSGNGLLLKGGKEAEHSNACLHGIIADAVFESTGGRVCREVIGLVVGRQAVAELLKLDALIDLVIPRGSGELVNRIKSTTKIPVLGHAEGVCHMFIDTAADEAKAIALAVDAKINYPAACNALETLLVHQDCV--ATGLAKKVADALVSAGGKVLGGPRAIAAGLA--TEPTPSFKTEYGENSL--SLEVVDDAGAAIDHIHRFGSGHTEAIVTEDAATAQSFLERVDSACVFHNASTRFSDGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS--SDAHTV 752          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A7S2WAW7_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WAW7_9STRA)

HSP 1 Score: 725 bits (1871), Expect = 3.610e-250
Identity = 395/714 (55.32%), Postives = 505/714 (70.73%), Query Frame = 0
Query:  107 SRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTF-DPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDD---EEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS 816
            SR  L +A RIV+KAGTS+V+N  G+  L+R+  +VEQ + L   G EV+L+SSGAVG GRQ+LRKQ  L  +    L       + TG   YDSACA+AGQLGLMSLY+TLF QCD+  SQ LVT +DF    R++HL    S +++ G+VPI+NENDAVSGN+GY  + +FSDND LA++VA  MNA +LILLTDV G++NKHP E GAKI +T+   +     IG KS  GRGGMGAK+ +A R +  GV ++VIASG +  SI  +  G  VGT+F   PG   D  D     D    +   A  AR   RAL AL +EER  +++ +A+AL   S EI  AN +D+  A  N++AP L+ RLKLTE K++ LA GI ++++ +EP+     R E+ +GL+L ++T PIGVLLI+FESRPDSLPQIA+LAIRSGNGLLLKGG+EAEHSNA LHRIIV AV   + G+V +D + LVTSR  V  LL LD  IDLV+PRGS +LV HIK  T+IPV+GH++GICHV++D++AD  KAV+IA+D+K +YP+ACNAAETILIH+A  E   GT   ++  L   GVT+ GG RAV LG++     +    TEYG  DLT  +E+V  ++ A+DHI   GSSHTE IVTED + AK+FL  VD+ACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTK++L S
Sbjct:   23 SRLDLRNARRIVIKAGTSVVTNPDGHFSLTRLSGIVEQISELIHQGFEVVLISSGAVGCGRQVLRKQAFLSGNFKQQL--RTSEDRSTG---YDSACASAGQLGLMSLYDTLFGQCDICASQFLVTSYDFTDDSRKQHLVECMSRIIEHGMVPIVNENDAVSGNKGYTTENVFSDNDALASIVAANMNADLLILLTDVPGLFNKHPSEPGAKIINTYYSGDASNFTIGAKSTQGRGGMGAKVDSAVRGLDRGVKAIVIASGYHQRSIFDVCLGYPVGTLFL--PGSSPDPKDALCSADGPPSLNSMAVEARKAARALQALSTEERVRVMKAIAQALRDNSDEILEANAKDLALAVTNDVAPPLVQRLKLTEQKLETLAAGIEAISEADEPIGHVEGRTELADGLILSKVTVPIGVLLIIFESRPDSLPQIAALAIRSGNGLLLKGGREAEHSNATLHRIIVTAVQTATAGKVSQDLVSLVTSREQVSSLLALDDCIDLVVPRGSKALVEHIKQHTRIPVLGHADGICHVFVDESADLEKAVDIAIDSKTDYPAACNAAETILIHEAAAETMGGT---ILGGLRRAGVTIKGGARAVALGMIPHSDAAESMSTEYG--DLTCLVEVVSGIDEAIDHIHSHGSSHTECIVTEDAEQAKVFLARVDAACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKYILES 724          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: W7U194_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U194_9STRA)

HSP 1 Score: 722 bits (1863), Expect = 9.390e-249
Identity = 407/756 (53.84%), Postives = 516/756 (68.25%), Query Frame = 0
Query:  108 RAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTF----DPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDD---------------------------EEDADDMIVRQAE--------MARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV 824
            R  L  A R+V+KAGTS+VS   GYP L R+  +VEQ   L K G++V+LV+SGAVG+G+QLL KQQ +  S+  V+ GH  + K  G   YDSACAAAGQLGLMSLYETLF+   +ATSQ+LVT +DF   +RR H++Y  +++L+LG+VPI+NENDAVSGNEGY    +FSDND LA+LVA++  A +LILLTDV GVY+K P +E AK    +     P      IGEKSA GRGGM AKI AA+ A  GGV  VVIASG     I +I  GE  GT+FC    L+   +D                           +E  +D++  +AE          +   RAL +L   ER+ IL  VA+ALLA +                  +A  LLNRL LT++K+  LA+GI +LA+ EEP+ +  SR+EV  GL L Q T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSN  LHR+I DA+   S+GRVP + IGLVT+R+ V  LL+LD +IDLVIPRGS +LV  IK+ST+IPV+GH+EG+CH+Y+DK AD  KA  I VDAK +YP+ACNA ET+L+H++ + +GA   ++++RAL S GVTVLGGP AV  G+V     +   + EYG  DLT+++E+V  +E A+ HI  FGSSHT+ I+TED   A+ FL +VDSACVFHNAS+RF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW+LRS G  +HTV
Sbjct:    6 RLRLRDAQRVVIKAGTSVVSTPEGYPSLVRLAHIVEQICLLIKEGRDVLLVTSGAVGIGQQLLAKQQRMSLSVRDVVSGHPPIKKPNG---YDSACAAAGQLGLMSLYETLFSTRQIATSQILVTGYDFTCSDRREHVQYCVNSLLQLGIVPIINENDAVSGNEGYTESTVFSDNDSLASLVAQETQADLLILLTDVCGVYDKPPSDEDAKQLGLYLRDCSPG-----IGEKSAQGRGGMQAKIDAATAAAKGGVEHVVIASGHEAEVIGKIARGENTGTLFC-RAALFPPEEDMSASTGSLVGLNGNGDGPGTVCSSGPPDELLEDILALEAEGGIRGQALATKEAQRALASLTGNERSQILLAVADALLANTAXXXXXXXXXXXXXXXXXLAEPLLNRLGLTKEKLMTLADGIRALANMEEPVGRLVSRMEVAAGLELTQTTVPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNQVLHRVITDAMTTASEGRVPAEAIGLVTTRSEVSALLELDDVIDLVIPRGSSALVRSIKSSTRIPVLGHAEGVCHIYVDKGADLRKAARIVVDAKTDYPAACNAVETLLLHRSFVADGAIGASKLLRALMSAGVTVLGGPEAVKEGLVSPGHAAKDLRQEYG--DLTITVEVVADMEEAIAHIHKFGSSHTDAIITEDPVCAEEFLRSVDSACVFHNASSRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRSSG--SHTV 748          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A1W0A8G2_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A8G2_9STRA)

HSP 1 Score: 706 bits (1821), Expect = 4.920e-243
Identity = 394/730 (53.97%), Postives = 522/730 (71.51%), Query Frame = 0
Query:  111 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRH-ELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGK----KDPYLLKPM 835
              +A RIV+K GTS+VS S G   L R+ ++ EQ + LK+ GKEV+LV+SG++GVGR+ L+KQ LL +S+   + G++ + +   ++    A AAAGQ+GLMSLYETLF+  DVA SQ+LVTE DF+T E R ++R T   +L+L V+PI+NENDAVS     E + +F+DND LAALVA ++ A +LILLTDV+G+YNK P E  AKI   F P +  + +IGEKS+ GRGGMG+KI++A  A+  GV +VVIASG     ++ I+ G  +GT+F  +P L  ++  +  A+DM +     AR G RAL  L S++RA ILRK+A +L+  S  I  AN+ D+  A+A+++  AL  RLKLT+ K++ LA+GI S+AD  EP+ +  S++EV  GLVL+Q+T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNACLH++I DA+   ++GRVPR  IGLVT+R  V ELLKLDH+IDL IPRGS SLV +IK +T+IPV+GH+EG+CH+YI   A+  KA  + VDAK +YP+ACNA ET+L+ K  ++ GA     ++ ALH  GV +L GP A  LGV+   + +P    EYG   LT+ + I   VE A+ HI    S HTE+IVTED++VA+ FL+ +DSACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKW LRS+  S HTV       + Y  KP+
Sbjct:   12 FQNAKRIVIKMGTSVVS-SNGEAALGRVASLAEQISMLKRQGKEVLLVTSGSIGVGRKKLQKQILLSSSLRMHVQGNSNMSRLLEKKK--GAMAAAGQVGLMSLYETLFSLYDVACSQVLVTESDFKTKEHRANIRDTLLHLLELDVIPIVNENDAVSAGVIREGETVFTDNDSLAALVAGEIKANLLILLTDVDGLYNKPPSEPNAKIISVFVPEQPGQFVIGEKSSVGRGGMGSKIQSAQGAIDNGVNAVVIASGFKYGVLDAIMKGHNIGTLFVQHPELLVEN--KSSAEDMAIS----AREGSRALLQLTSDDRARILRKIAASLIENSDTILYANQLDM--AEASSVDAALRARLKLTKPKLETLAKGIRSIADQVEPIGQTVSKMEVSSGLVLEQLTCPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEASHSNACLHKLIGDAIESETQGRVPRGVIGLVTTRDDVSELLKLDHVIDLCIPRGSNSLVTYIKKNTRIPVLGHAEGVCHMYIHSGANMEKAKSLVVDAKTDYPAACNALETLLVDKVHVDSGA--IQSIIDALHDAGVKILVGPTAASLGVLPEAEAAPSLSHEYGAMALTLEV-ISGGVEKAIAHINAHSSGHTESIVTEDKNVAEQFLDGIDSACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLRSN--SVHTVASFTNGHNKYTHKPL 725          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A7S2BH10_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2BH10_9STRA)

HSP 1 Score: 703 bits (1814), Expect = 1.320e-241
Identity = 393/716 (54.89%), Postives = 498/716 (69.55%), Query Frame = 0
Query:  111 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMG------AVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFH-TFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERI-VCGERVGTMFCANP-GLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLAD-GEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS 816
            ++ A R+V+KAGTS+VSN  G   L R+ A+VEQ   L K+ KEVIL+SSGAVG GR+ LRKQ LL TS+       + LD  A+  +      Y +A A++GQL LM +YET+F+Q DVA SQ LVTE+DF    RR HL+ +   ++K  +VPI+NENDAVS N+GY  D +FSDND LAALVA+QM A  L+LLTDV+G+Y++ P E  A +    +     E   G KS  GRGGM AK+ AA  A+  GV +VV+ASG +   ++++ V GE  GT+F   P    E +           +QA  AR  GR L  L   ER+G+L+ VA+A+      I +AN RD+ AAK NN+APALL RLKL+ +K+  +A GI  LAD   +PL K   R EV EGL L Q++ PIGVLLIVFESRPDSLPQIASLA+ SGNGLLLKGGKEAEHSN  LH+++VDAVA  S G+VPR+ +GLVTSR  V +LLKLD LIDLVIPRGS +LV +IK +T+IPV+GH++GICH YID+ AD  KA  IAVD K+NYP+ACNA ET+LIH+A ++ G   CA V+R L   GV++ GGP A+  G++     +     EYG  DLT  +E+V ++E A+ HI   GSSHTE IVTED   A+ F++T D+ACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKWVLRS
Sbjct:   19 IVDAKRVVIKAGTSVVSNEDGTLSLVRVAAIVEQVYALLKLQKEVILISSGAVGCGRRKLRKQSLLGTSLRKQMHSCSSLDDLADEDES---EFYSAASASSGQLELMKVYETMFSQFDVAASQFLVTEYDFNNSSRRSHLKNSMEEIIKHCMVPIINENDAVSANQGYTDDNIFSDNDALAALVAQQMGADALVLLTDVDGLYDRAPSEPDATVIPIVYRSAGDEFSYGAKSNQGRGGMSAKVGAAMGALEAGVKAVVVASGKDTTVLQKLFVTGEGAGTLFLEQPPSTDEPAVGVSGLTPTSAQQAASARDAGRKLQGLSFSERSGVLKAVAQAIRDNEESILLANSRDMSAAKQNNLAPALLQRLKLSHEKLVTVARGIDQLADLAADPLGKVLGRTEVAEGLQLTQLSVPIGVLLIVFESRPDSLPQIASLALLSGNGLLLKGGKEAEHSNYTLHKVVVDAVAAASDGKVPREIVGLVTSRTQVSDLLKLDRLIDLVIPRGSKALVTNIKKNTRIPVLGHADGICHTYIDQHADDVKAAAIAVDGKINYPAACNATETVLIHQAKVDSGG--CATVMRELRKAGVSLKGGPNAIAQGLIDMSCAAKSLSVEYG--DLTCLVEVVANMEEAIAHIHEHGSSHTECIVTEDTATAEEFIKTTDAACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS 727          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A6A3JB98_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=24 Tax=Peronosporaceae TaxID=4777 RepID=A0A6A3JB98_9STRA)

HSP 1 Score: 699 bits (1805), Expect = 3.440e-240
Identity = 398/730 (54.52%), Postives = 513/730 (70.27%), Query Frame = 0
Query:  111 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADG-MFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQ-AEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGG-DKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV----GKKDPYLLK 833
            L  ATRIVVK GTS+VS + G P L RI ++VEQ   LK+ GKEV+LV+SGAVG+GR+ L KQ LL  S+   + G+ ++           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +     DG +F+DND LAALV  ++ A +L+LLTDVEG+YNK P + GAKI   F P   +  IGEKS+ GRGGMGAKI AA  A+S GV +VVIASG+    +  I+ G  +GT+F ANPG+       E  D M V + A  AR G   L AL SEERA IL ++A+ L   S  I  AN +D+ AA+ ++I   LL RLKL+E+K++ LA+GI S+A+ EEP+ +   R E+  GLVL Q T+ IGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNA LH+IIV+AV E + G+V +D IGLVTSR  + +LL+LD +IDL IPRGSGS+V++IK +T+IPV+GH+EG+CH+YI  AAD  KA+E+A+DAK +YP+ACNA ET+LI ++L+  G     +++ AL   G+ +   P A  LGV     + +    TEYG  DLT+  E+ +  +AA+ HI  + S HTE IVTED   A++F + +DSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW L S  ES HTV    G K+ + LK
Sbjct:   26 LKHATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLLVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQM---LALEKKKGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASP----DGTVFTDNDSLAALVGGEIEADLLMLLTDVEGLYNKPPTQPGAKIISVFRPENSDFKIGEKSSVGRGGMGAKIEAAQSAISQGVNAVVIASGMKYGVVGSIMKGSSLGTLFVANPGV-------ESPDCMSVEEMATAAREGCHQLQALSSEERANILFRIADELKKNSKTILNANRKDLLAAQKSSIDEGLLVRLKLSEEKLETLADGIRSIAEAEEPIGRMLKRTELASGLVLHQETASIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNAVLHKIIVNAVEEATNGKVKKDVIGLVTSREEISDLLRLDDVIDLCIPRGSGSMVSYIKKNTRIPVLGHAEGVCHMYIHGAADVKKAIELAIDAKTDYPAACNALETLLIDESLVTSGQDK--EILAALEEAGINLHIAPNAAKLGVATAKSRPTNSLSTEYGSKDLTI--EVANGADAAISHINQYSSGHTECIVTEDAAAAELFQQMIDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLVS--ESGHTVSQFSGAKNDHPLK 734          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A0N7L745_PLAHL (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0N7L745_PLAHL)

HSP 1 Score: 693 bits (1788), Expect = 1.100e-237
Identity = 389/721 (53.95%), Postives = 503/721 (69.76%), Query Frame = 0
Query:  108 RAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADG-MFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGG-DKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGK 826
            R  L +ATRIVVK GTS+VS + G P L RI ++VEQ   LK+ GKEV++V+SGAVG+GR+ L KQ LL  S+   + G+ ++           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +     DG +F+DND LAALV  ++ A +L+LLTDVEG+YNK P   GAK+   F P  +   IGEKS+ GRGGMGAKI+AA  A+S GV +VVIASG     +  IV G  +GT+F ANPG   +S D   A++M       AR G   L  L SEER  IL +VA+ L      I  AN +D+ AA+ ++I   L+ RLKLTE+K+  L +GI S+A+ EEP+ +   R E+  GL+L Q T+PIGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNA LHRIIV+AV E S+G+V +D IGLVTSR  + +LL +D++IDL IPRGSGS+V+HIK +T+IPV+GH+EG+CH+YI  AAD  K++E+A+DAK +YP+ACNA E++L+ +A++  G     +++  L    + +  GP A  LGV     + +    TEYG  DLT+  E+V  + AA+ HI  + S HTE IVTED   A+ F   VDSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW+L S  ES H V +
Sbjct:   19 RVDLKNATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLVVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQM---LALEKKQGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASP----DGTVFTDNDSLAALVGGEIGADLLMLLTDVEGLYNKPPTHPGAKVISVFIPENNNFRIGEKSSVGRGGMGAKIKAAQSAISQGVKAVVIASGFRYGVVASIVKGANLGTLFVANPGA--ESPDSMSAEEMAFS----ARKGSHQLQMLSSEERVHILFRVADDLKRNFSTILAANRQDLLAAQKSSIDEGLIARLKLTEEKLDTLIDGIRSIAESEEPIGRILKRTELASGLILHQETAPIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEARHSNAELHRIIVNAVEEASQGKVKKDVIGLVTSRDEISDLLCMDNVIDLCIPRGSGSMVSHIKKNTRIPVLGHAEGVCHMYIHSAADINKSIELAIDAKTDYPAACNALESLLLDEAVVSSGQDK--KILAKLEKADINLHIGPNAASLGVATAKSRPTDSLSTEYGSKDLTI--EVVHGLSAAISHINQYSSGHTECIVTEDAAAAETFQRRVDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWILAS--ESGHIVSQ 719          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: W4H1A3_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=10 Tax=Aphanomyces astaci TaxID=112090 RepID=W4H1A3_9STRA)

HSP 1 Score: 685 bits (1767), Expect = 7.730e-235
Identity = 385/726 (53.03%), Postives = 508/726 (69.97%), Query Frame = 0
Query:  114 ATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVA-EGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS---HGESAHTVGKKDPYLLKPM 835
            A RIV+K GTS+VS + G P L R+ ++VEQ + LK+ GKEV+LV+SG++G+GR+ L KQ LL  S+   + G  E       +    A AAAGQ+GLM+LYETLF+  DVA SQ+LVTE +FRTPE R ++R T   +L + V+PI+NENDA+S     + DG+F+DND LAAL+A + +A +L+LLTDV+GV++K P E  AK+   F      ++ GEKS+ GRGGM AKIR+A RA++ GV +VVIASG     ++ I+ G  VGT+F  NP      +  ED        A  AR G RAL  L S ER+ I+ ++++ L+ +SP I  AN +D+ AA ++++ P L +RLKLT DK+  L++GI S+A  +EPL +  S +EV  GLVL+Q+T+PIGVLL+VFESRPDSLPQIA+LA+RSGNGLLLKGGKEA+HSNACLH ++ DA+  E SK  VP   +GLVT+R  VG LL LDH++DL IPRGS SLV+HIK +T+IPV+GH+EG+CHVYI   AD  KA ++AVDAK +YP+ACNA ET+LI +A++E GA     ++ AL + GVT+  GPRA  L +V G   +P    EYG   LTV + +   V+ A+ HI  + S HTE I+TED  VA+ FL  +DSACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGV+GLLTTKW LRS   H  +A   G  D Y  K +
Sbjct:   16 AKRIVIKMGTSVVSTN-GEPALGRLASLVEQVSMLKRQGKEVLLVTSGSIGIGRKKLHKQLLLSASLRMHVQGGTEQHHALDAKK--GAMAAAGQVGLMALYETLFSMYDVACSQVLVTEAEFRTPEHRANIRDTLLHLLDMDVLPIVNENDAISAGLYRDEDGVFTDNDSLAALIAGETSANLLVLLTDVDGVFDKPPTEPHAKVLSVFGAGG-PIVFGEKSSVGRGGMQAKIRSAQRALAQGVNAVVIASGFKYGILDSIMKGTVVGTLFVPNPSALTPVNSPED-------MAVAARDGSRALLELSSAERSSIVLRLSQLLVDESPRILEANRKDLDAA-SSSLDPQLRSRLKLTHDKLVTLSQGIASVAGQDEPLGRVLSLMEVSSGLVLEQVTAPIGVLLVVFESRPDSLPQIAALALRSGNGLLLKGGKEAKHSNACLHALVQDAIRLETSK--VPPSVVGLVTTRDDVGALLALDHVVDLCIPRGSNSLVSHIKKNTRIPVLGHAEGVCHVYIHDNADLVKATKVAVDAKTDYPAACNALETLLIDEAIVERGAHHA--ILDALVAAGVTLYEGPRAAKLHLVPGALAAPSLSHEYGSLGLTVEV-VAGGVDGAMAHINRYSSGHTECIITEDAAVAETFLNGIDSACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVQGLLTTKWKLRSAKVHTVAAFGQGGTDTYTHKTL 724          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A6H5JBC7_9PHAE (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JBC7_9PHAE)

HSP 1 Score: 1595 bits (4129), Expect = 0.000e+0
Identity = 842/842 (100.00%), Postives = 842/842 (100.00%), Query Frame = 2
Query:  149 MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 2674
            MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW
Sbjct:    1 MSGFPPPSNSLPPVNIGQSASTAGGGGSVXXXXXXXXXXXXXXXXTTVPVADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGKSGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 842          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: D7G5M6_ECTSI (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5M6_ECTSI)

HSP 1 Score: 1184 bits (3063), Expect = 0.000e+0
Identity = 619/663 (93.36%), Postives = 622/663 (93.82%), Query Frame = 2
Query:  686 MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW 2674
            MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLT+DKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIK                                    VNYPSACNAAETILIHKALLEEGAGTC+QVVRALHS GVTVLGGPRAVDLGVVGGDKISPGFKTEYG  DLTVSLEIVDSVEAALDHIQ FGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGE+AHTVGKKDPY LKPMDLEAGAW
Sbjct:    1 MGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTKDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIK------------------------------------VNYPSACNAAETILIHKALLEEGAGTCSQVVRALHSAGVTVLGGPRAVDLGVVGGDKISPGFKTEYG--DLTVSLEIVDSVEAALDHIQRFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGETAHTVGKKDPYSLKPMDLEAGAW 625          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A835ZBW4_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBW4_9STRA)

HSP 1 Score: 884 bits (2283), Expect = 7.110e-301
Identity = 477/728 (65.52%), Postives = 565/728 (77.61%), Query Frame = 2
Query:  467 SRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGR-------RNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLY--EDSDDEEDADDMIVR-QAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV 2620
            SR  ++ A RIV+KAGTS+VS+ RGYP L+R+GA+VEQCAWL+KMGKEV+LVSSGAVGVGRQLLRKQQLL++S+G VLDGH     G G         +Y+SACAAAGQLGLMSLY+TLF+QCD ATSQ+L+T  DF TPER R+LRYT +TML+LGV+PI+NENDAVS N+GY  DG FSDNDGLAA+VA+QM A++L+LLTDV+GVY+K P   GA+I HT+ P   E+ IG+KS GGRGGM AKI AA RAV  GV SVVIA+G NP+SIE++ CGE+VGT+FC N       D  +E++A    VR  AE AR GGRALC L +EER   L  VA+AL A+  E+  AN RD++ A+   +A  LL RL+LT  KI+ L +GI SLAD  EP+ KC S LEV EGLVLKQ+T+PIGVLL+VFESRPDSLPQIASLA+RSGNGLLLKGGKEAEHSNACLH II DAV E + GRV R+ IGLV  R AV ELLKLD LIDLVIPRGSG LVN IK++TKIPV+GH+EG+CH++ID AAD  KA+ +AVDAK+NYP+ACNA ET+L+H+  +    G   +V  AL S G  VLGGPRA+  G+    + +P FKTEYGE  L  SLE+VD   AA+DHI  FGS HTE IVTED   A+ FLE VDSACVFHNASTRFSDG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKWVLRS    AHTV
Sbjct:   38 SRQKVLHAHRIVIKAGTSVVSDERGYPSLTRLGAIVEQCAWLRKMGKEVLLVSSGAVGVGRQLLRKQQLLNSSLGQVLDGH----DGNGAALLQAKSNSYNSACAAAGQLGLMSLYDTLFSQCDTATSQMLLTADDFGTPERCRNLRYTMNTMLQLGVIPIVNENDAVSANQGYTKDGTFSDNDGLAAVVAQQMGAQLLVLLTDVDGVYDKAPTLPGARIIHTYLPAT-EVQIGDKSVGGRGGMSAKISAAMRAVEAGVASVVIANGCNPFSIEQVCCGEKVGTLFCQNAAAMAARDLPEEDEALTEKVRVAAEAARAGGRALCQLSTEERTQALHAVAKALGARQAEVLAANARDMQEAERVKLAGPLLKRLQLTPAKIQTLVDGINSLADQPEPIGKCMSHLEVSEGLVLKQVTTPIGVLLVVFESRPDSLPQIASLALRSGNGLLLKGGKEAEHSNACLHGIIADAVFESTGGRVCREVIGLVVGRQAVAELLKLDALIDLVIPRGSGELVNRIKSTTKIPVLGHAEGVCHMFIDTAADEAKAIALAVDAKINYPAACNALETLLVHQDCV--ATGLAKKVADALVSAGGKVLGGPRAIAAGLA--TEPTPSFKTEYGENSL--SLEVVDDAGAAIDHIHRFGSGHTEAIVTEDAATAQSFLERVDSACVFHNASTRFSDGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS--SDAHTV 752          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A7S2WAW7_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WAW7_9STRA)

HSP 1 Score: 725 bits (1871), Expect = 9.050e-240
Identity = 395/714 (55.32%), Postives = 505/714 (70.73%), Query Frame = 2
Query:  467 SRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTF-DPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDD---EEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS 2596
            SR  L +A RIV+KAGTS+V+N  G+  L+R+  +VEQ + L   G EV+L+SSGAVG GRQ+LRKQ  L  +    L       + TG   YDSACA+AGQLGLMSLY+TLF QCD+  SQ LVT +DF    R++HL    S +++ G+VPI+NENDAVSGN+GY  + +FSDND LA++VA  MNA +LILLTDV G++NKHP E GAKI +T+   +     IG KS  GRGGMGAK+ +A R +  GV ++VIASG +  SI  +  G  VGT+F   PG   D  D     D    +   A  AR   RAL AL +EER  +++ +A+AL   S EI  AN +D+  A  N++AP L+ RLKLTE K++ LA GI ++++ +EP+     R E+ +GL+L ++T PIGVLLI+FESRPDSLPQIA+LAIRSGNGLLLKGG+EAEHSNA LHRIIV AV   + G+V +D + LVTSR  V  LL LD  IDLV+PRGS +LV HIK  T+IPV+GH++GICHV++D++AD  KAV+IA+D+K +YP+ACNAAETILIH+A  E   GT   ++  L   GVT+ GG RAV LG++     +    TEYG  DLT  +E+V  ++ A+DHI   GSSHTE IVTED + AK+FL  VD+ACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTK++L S
Sbjct:   23 SRLDLRNARRIVIKAGTSVVTNPDGHFSLTRLSGIVEQISELIHQGFEVVLISSGAVGCGRQVLRKQAFLSGNFKQQL--RTSEDRSTG---YDSACASAGQLGLMSLYDTLFGQCDICASQFLVTSYDFTDDSRKQHLVECMSRIIEHGMVPIVNENDAVSGNKGYTTENVFSDNDALASIVAANMNADLLILLTDVPGLFNKHPSEPGAKIINTYYSGDASNFTIGAKSTQGRGGMGAKVDSAVRGLDRGVKAIVIASGYHQRSIFDVCLGYPVGTLFL--PGSSPDPKDALCSADGPPSLNSMAVEARKAARALQALSTEERVRVMKAIAQALRDNSDEILEANAKDLALAVTNDVAPPLVQRLKLTEQKLETLAAGIEAISEADEPIGHVEGRTELADGLILSKVTVPIGVLLIIFESRPDSLPQIAALAIRSGNGLLLKGGREAEHSNATLHRIIVTAVQTATAGKVSQDLVSLVTSREQVSSLLALDDCIDLVVPRGSKALVEHIKQHTRIPVLGHADGICHVFVDESADLEKAVDIAIDSKTDYPAACNAAETILIHEAAAETMGGT---ILGGLRRAGVTIKGGARAVALGMIPHSDAAESMSTEYG--DLTCLVEVVSGIDEAIDHIHSHGSSHTECIVTEDAEQAKVFLARVDAACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKYILES 724          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: W7U194_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U194_9STRA)

HSP 1 Score: 722 bits (1863), Expect = 2.230e-238
Identity = 407/756 (53.84%), Postives = 516/756 (68.25%), Query Frame = 2
Query:  470 RAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTF----DPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDD---------------------------EEDADDMIVRQAE--------MARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV 2620
            R  L  A R+V+KAGTS+VS   GYP L R+  +VEQ   L K G++V+LV+SGAVG+G+QLL KQQ +  S+  V+ GH  + K  G   YDSACAAAGQLGLMSLYETLF+   +ATSQ+LVT +DF   +RR H++Y  +++L+LG+VPI+NENDAVSGNEGY    +FSDND LA+LVA++  A +LILLTDV GVY+K P +E AK    +     P      IGEKSA GRGGM AKI AA+ A  GGV  VVIASG     I +I  GE  GT+FC    L+   +D                           +E  +D++  +AE          +   RAL +L   ER+ IL  VA+ALLA +                  +A  LLNRL LT++K+  LA+GI +LA+ EEP+ +  SR+EV  GL L Q T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSN  LHR+I DA+   S+GRVP + IGLVT+R+ V  LL+LD +IDLVIPRGS +LV  IK+ST+IPV+GH+EG+CH+Y+DK AD  KA  I VDAK +YP+ACNA ET+L+H++ + +GA   ++++RAL S GVTVLGGP AV  G+V     +   + EYG  DLT+++E+V  +E A+ HI  FGSSHT+ I+TED   A+ FL +VDSACVFHNAS+RF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW+LRS G  +HTV
Sbjct:    6 RLRLRDAQRVVIKAGTSVVSTPEGYPSLVRLAHIVEQICLLIKEGRDVLLVTSGAVGIGQQLLAKQQRMSLSVRDVVSGHPPIKKPNG---YDSACAAAGQLGLMSLYETLFSTRQIATSQILVTGYDFTCSDRREHVQYCVNSLLQLGIVPIINENDAVSGNEGYTESTVFSDNDSLASLVAQETQADLLILLTDVCGVYDKPPSDEDAKQLGLYLRDCSPG-----IGEKSAQGRGGMQAKIDAATAAAKGGVEHVVIASGHEAEVIGKIARGENTGTLFC-RAALFPPEEDMSASTGSLVGLNGNGDGPGTVCSSGPPDELLEDILALEAEGGIRGQALATKEAQRALASLTGNERSQILLAVADALLANTAXXXXXXXXXXXXXXXXXLAEPLLNRLGLTKEKLMTLADGIRALANMEEPVGRLVSRMEVAAGLELTQTTVPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNQVLHRVITDAMTTASEGRVPAEAIGLVTTRSEVSALLELDDVIDLVIPRGSSALVRSIKSSTRIPVLGHAEGVCHIYVDKGADLRKAARIVVDAKTDYPAACNAVETLLLHRSFVADGAIGASKLLRALMSAGVTVLGGPEAVKEGLVSPGHAAKDLRQEYG--DLTITVEVVADMEEAIAHIHKFGSSHTDAIITEDPVCAEEFLRSVDSACVFHNASSRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWLLRSSG--SHTV 748          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A1W0A8G2_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A8G2_9STRA)

HSP 1 Score: 707 bits (1824), Expect = 3.160e-233
Identity = 394/730 (53.97%), Postives = 522/730 (71.51%), Query Frame = 2
Query:  479 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRH-ELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGK----KDPYLLKPM 2653
              +A RIV+K GTS+VS S G   L R+ ++ EQ + LK+ GKEV+LV+SG++GVGR+ L+KQ LL +S+   + G++ + +   ++    A AAAGQ+GLMSLYETLF+  DVA SQ+LVTE DF+T E R ++R T   +L+L V+PI+NENDAVS     E + +F+DND LAALVA ++ A +LILLTDV+G+YNK P E  AKI   F P +  + +IGEKS+ GRGGMG+KI++A  A+  GV +VVIASG     ++ I+ G  +GT+F  +P L  ++  +  A+DM +     AR G RAL  L S++RA ILRK+A +L+  S  I  AN+ D+  A+A+++  AL  RLKLT+ K++ LA+GI S+AD  EP+ +  S++EV  GLVL+Q+T PIGVLLIVFESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNACLH++I DA+   ++GRVPR  IGLVT+R  V ELLKLDH+IDL IPRGS SLV +IK +T+IPV+GH+EG+CH+YI   A+  KA  + VDAK +YP+ACNA ET+L+ K  ++ GA     ++ ALH  GV +L GP A  LGV+   + +P    EYG   LT+ + I   VE A+ HI    S HTE+IVTED++VA+ FL+ +DSACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKW LRS+  S HTV       + Y  KP+
Sbjct:   12 FQNAKRIVIKMGTSVVS-SNGEAALGRVASLAEQISMLKRQGKEVLLVTSGSIGVGRKKLQKQILLSSSLRMHVQGNSNMSRLLEKKK--GAMAAAGQVGLMSLYETLFSLYDVACSQVLVTESDFKTKEHRANIRDTLLHLLELDVIPIVNENDAVSAGVIREGETVFTDNDSLAALVAGEIKANLLILLTDVDGLYNKPPSEPNAKIISVFVPEQPGQFVIGEKSSVGRGGMGSKIQSAQGAIDNGVNAVVIASGFKYGVLDAIMKGHNIGTLFVQHPELLVEN--KSSAEDMAIS----AREGSRALLQLTSDDRARILRKIAASLIENSDTILYANQLDM--AEASSVDAALRARLKLTKPKLETLAKGIRSIADQVEPIGQTVSKMEVSSGLVLEQLTCPIGVLLIVFESRPDSLPQIAALALRSGNGLLLKGGKEASHSNACLHKLIGDAIESETQGRVPRGVIGLVTTRDDVSELLKLDHVIDLCIPRGSNSLVTYIKKNTRIPVLGHAEGVCHMYIHSGANMEKAKSLVVDAKTDYPAACNALETLLVDKVHVDSGA--IQSIIDALHDAGVKILVGPTAASLGVLPEAEAAPSLSHEYGAMALTLEV-ISGGVEKAIAHINAHSSGHTESIVTEDKNVAEQFLDGIDSACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLRSN--SVHTVASFTNGHNKYTHKPL 725          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A7S2BH10_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2BH10_9STRA)

HSP 1 Score: 703 bits (1814), Expect = 2.240e-231
Identity = 393/716 (54.89%), Postives = 498/716 (69.55%), Query Frame = 2
Query:  479 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMG------AVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFH-TFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERI-VCGERVGTMFCANP-GLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLAD-GEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS 2596
            ++ A R+V+KAGTS+VSN  G   L R+ A+VEQ   L K+ KEVIL+SSGAVG GR+ LRKQ LL TS+       + LD  A+  +      Y +A A++GQL LM +YET+F+Q DVA SQ LVTE+DF    RR HL+ +   ++K  +VPI+NENDAVS N+GY  D +FSDND LAALVA+QM A  L+LLTDV+G+Y++ P E  A +    +     E   G KS  GRGGM AK+ AA  A+  GV +VV+ASG +   ++++ V GE  GT+F   P    E +           +QA  AR  GR L  L   ER+G+L+ VA+A+      I +AN RD+ AAK NN+APALL RLKL+ +K+  +A GI  LAD   +PL K   R EV EGL L Q++ PIGVLLIVFESRPDSLPQIASLA+ SGNGLLLKGGKEAEHSN  LH+++VDAVA  S G+VPR+ +GLVTSR  V +LLKLD LIDLVIPRGS +LV +IK +T+IPV+GH++GICH YID+ AD  KA  IAVD K+NYP+ACNA ET+LIH+A ++ G   CA V+R L   GV++ GGP A+  G++     +     EYG  DLT  +E+V ++E A+ HI   GSSHTE IVTED   A+ F++T D+ACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGVEGLLTTKWVLRS
Sbjct:   19 IVDAKRVVIKAGTSVVSNEDGTLSLVRVAAIVEQVYALLKLQKEVILISSGAVGCGRRKLRKQSLLGTSLRKQMHSCSSLDDLADEDES---EFYSAASASSGQLELMKVYETMFSQFDVAASQFLVTEYDFNNSSRRSHLKNSMEEIIKHCMVPIINENDAVSANQGYTDDNIFSDNDALAALVAQQMGADALVLLTDVDGLYDRAPSEPDATVIPIVYRSAGDEFSYGAKSNQGRGGMSAKVGAAMGALEAGVKAVVVASGKDTTVLQKLFVTGEGAGTLFLEQPPSTDEPAVGVSGLTPTSAQQAASARDAGRKLQGLSFSERSGVLKAVAQAIRDNEESILLANSRDMSAAKQNNLAPALLQRLKLSHEKLVTVARGIDQLADLAADPLGKVLGRTEVAEGLQLTQLSVPIGVLLIVFESRPDSLPQIASLALLSGNGLLLKGGKEAEHSNYTLHKVVVDAVAAASDGKVPREIVGLVTSRTQVSDLLKLDRLIDLVIPRGSKALVTNIKKNTRIPVLGHADGICHTYIDQHADDVKAAAIAVDGKINYPAACNATETVLIHQAKVDSGG--CATVMRELRKAGVSLKGGPNAIAQGLIDMSCAAKSLSVEYG--DLTCLVEVVANMEEAIAHIHEHGSSHTECIVTEDTATAEEFIKTTDAACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVEGLLTTKWVLRS 727          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A6A3JB98_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=24 Tax=Peronosporaceae TaxID=4777 RepID=A0A6A3JB98_9STRA)

HSP 1 Score: 701 bits (1808), Expect = 1.980e-230
Identity = 398/730 (54.52%), Postives = 513/730 (70.27%), Query Frame = 2
Query:  479 LMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADG-MFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQ-AEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGG-DKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTV----GKKDPYLLK 2647
            L  ATRIVVK GTS+VS + G P L RI ++VEQ   LK+ GKEV+LV+SGAVG+GR+ L KQ LL  S+   + G+ ++           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +     DG +F+DND LAALV  ++ A +L+LLTDVEG+YNK P + GAKI   F P   +  IGEKS+ GRGGMGAKI AA  A+S GV +VVIASG+    +  I+ G  +GT+F ANPG+       E  D M V + A  AR G   L AL SEERA IL ++A+ L   S  I  AN +D+ AA+ ++I   LL RLKL+E+K++ LA+GI S+A+ EEP+ +   R E+  GLVL Q T+ IGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNA LH+IIV+AV E + G+V +D IGLVTSR  + +LL+LD +IDL IPRGSGS+V++IK +T+IPV+GH+EG+CH+YI  AAD  KA+E+A+DAK +YP+ACNA ET+LI ++L+  G     +++ AL   G+ +   P A  LGV     + +    TEYG  DLT+  E+ +  +AA+ HI  + S HTE IVTED   A++F + +DSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW L S  ES HTV    G K+ + LK
Sbjct:   26 LKHATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLLVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQM---LALEKKKGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASP----DGTVFTDNDSLAALVGGEIEADLLMLLTDVEGLYNKPPTQPGAKIISVFRPENSDFKIGEKSSVGRGGMGAKIEAAQSAISQGVNAVVIASGMKYGVVGSIMKGSSLGTLFVANPGV-------ESPDCMSVEEMATAAREGCHQLQALSSEERANILFRIADELKKNSKTILNANRKDLLAAQKSSIDEGLLVRLKLSEEKLETLADGIRSIAEAEEPIGRMLKRTELASGLVLHQETASIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEALHSNAVLHKIIVNAVEEATNGKVKKDVIGLVTSREEISDLLRLDDVIDLCIPRGSGSMVSYIKKNTRIPVLGHAEGVCHMYIHGAADVKKAIELAIDAKTDYPAACNALETLLIDESLVTSGQDK--EILAALEEAGINLHIAPNAAKLGVATAKSRPTNSLSTEYGSKDLTI--EVANGADAAISHINQYSSGHTECIVTEDAAAAELFQQMIDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWKLVS--ESGHTVSQFSGAKNDHPLK 734          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: A0A0N7L745_PLAHL (Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0N7L745_PLAHL)

HSP 1 Score: 693 bits (1788), Expect = 1.560e-227
Identity = 389/721 (53.95%), Postives = 503/721 (69.76%), Query Frame = 2
Query:  470 RAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADG-MFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGG-DKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRSHGESAHTVGK 2626
            R  L +ATRIVVK GTS+VS + G P L RI ++VEQ   LK+ GKEV++V+SGAVG+GR+ L KQ LL  S+   + G+ ++           A AAAGQ+GLMSLYETLF+  DVA SQ+LVT  DF+T + R ++R T   +L+L VVPI+NENDAVS +     DG +F+DND LAALV  ++ A +L+LLTDVEG+YNK P   GAK+   F P  +   IGEKS+ GRGGMGAKI+AA  A+S GV +VVIASG     +  IV G  +GT+F ANPG   +S D   A++M       AR G   L  L SEER  IL +VA+ L      I  AN +D+ AA+ ++I   L+ RLKLTE+K+  L +GI S+A+ EEP+ +   R E+  GL+L Q T+PIGVLL++FESRPDSLPQIA+LA+RSGNGLLLKGGKEA HSNA LHRIIV+AV E S+G+V +D IGLVTSR  + +LL +D++IDL IPRGSGS+V+HIK +T+IPV+GH+EG+CH+YI  AAD  K++E+A+DAK +YP+ACNA E++L+ +A++  G     +++  L    + +  GP A  LGV     + +    TEYG  DLT+  E+V  + AA+ HI  + S HTE IVTED   A+ F   VDSACVFHNASTRF+DGYRFGLGAEVG+STGRIHARGPVGVEGLLTTKW+L S  ES H V +
Sbjct:   19 RVDLKNATRIVVKMGTSVVSTN-GEPALGRIASIVEQVCMLKRQGKEVLVVTSGAVGIGRKRLNKQILLSASLRTHVQGNQQM---LALEKKQGAMAAAGQIGLMSLYETLFSLYDVACSQVLVTASDFKTAQNRANMRDTMLNLLELDVVPIVNENDAVSASP----DGTVFTDNDSLAALVGGEIGADLLMLLTDVEGLYNKPPTHPGAKVISVFIPENNNFRIGEKSSVGRGGMGAKIKAAQSAISQGVKAVVIASGFRYGVVASIVKGANLGTLFVANPGA--ESPDSMSAEEMAFS----ARKGSHQLQMLSSEERVHILFRVADDLKRNFSTILAANRQDLLAAQKSSIDEGLIARLKLTEEKLDTLIDGIRSIAESEEPIGRILKRTELASGLILHQETAPIGVLLVIFESRPDSLPQIAALALRSGNGLLLKGGKEARHSNAELHRIIVNAVEEASQGKVKKDVIGLVTSRDEISDLLCMDNVIDLCIPRGSGSMVSHIKKNTRIPVLGHAEGVCHMYIHSAADINKSIELAIDAKTDYPAACNALESLLLDEAVVSSGQDK--KILAKLEKADINLHIGPNAASLGVATAKSRPTDSLSTEYGSKDLTI--EVVHGLSAAISHINQYSSGHTECIVTEDAAAAETFQRRVDSACVFHNASTRFADGYRFGLGAEVGISTGRIHARGPVGVEGLLTTKWILAS--ESGHIVSQ 719          
BLAST of EsuBft1017_6a-0001 vs. uniprot
Match: W4H1A3_9STRA (Delta-1-pyrroline-5-carboxylate synthase n=10 Tax=Aphanomyces astaci TaxID=112090 RepID=W4H1A3_9STRA)

HSP 1 Score: 686 bits (1770), Expect = 3.370e-225
Identity = 385/726 (53.03%), Postives = 508/726 (69.97%), Query Frame = 2
Query:  488 ATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKKMGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYDSACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTSTMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLILLTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAASRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEEDADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANERDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRLEVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKEAEHSNACLHRIIVDAVA-EGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDLVIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAKVNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDLGVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIVTEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARGPVGVEGLLTTKWVLRS---HGESAHTVGKKDPYLLKPM 2653
            A RIV+K GTS+VS + G P L R+ ++VEQ + LK+ GKEV+LV+SG++G+GR+ L KQ LL  S+   + G  E       +    A AAAGQ+GLM+LYETLF+  DVA SQ+LVTE +FRTPE R ++R T   +L + V+PI+NENDA+S     + DG+F+DND LAAL+A + +A +L+LLTDV+GV++K P E  AK+   F      ++ GEKS+ GRGGM AKIR+A RA++ GV +VVIASG     ++ I+ G  VGT+F  NP      +  ED        A  AR G RAL  L S ER+ I+ ++++ L+ +SP I  AN +D+ AA ++++ P L +RLKLT DK+  L++GI S+A  +EPL +  S +EV  GLVL+Q+T+PIGVLL+VFESRPDSLPQIA+LA+RSGNGLLLKGGKEA+HSNACLH ++ DA+  E SK  VP   +GLVT+R  VG LL LDH++DL IPRGS SLV+HIK +T+IPV+GH+EG+CHVYI   AD  KA ++AVDAK +YP+ACNA ET+LI +A++E GA     ++ AL + GVT+  GPRA  L +V G   +P    EYG   LTV + +   V+ A+ HI  + S HTE I+TED  VA+ FL  +DSACVFHNASTRF+DG+RFGLGAEVG+STGRIHARGPVGV+GLLTTKW LRS   H  +A   G  D Y  K +
Sbjct:   16 AKRIVIKMGTSVVSTN-GEPALGRLASLVEQVSMLKRQGKEVLLVTSGSIGIGRKKLHKQLLLSASLRMHVQGGTEQHHALDAKK--GAMAAAGQVGLMALYETLFSMYDVACSQVLVTEAEFRTPEHRANIRDTLLHLLDMDVLPIVNENDAISAGLYRDEDGVFTDNDSLAALIAGETSANLLVLLTDVDGVFDKPPTEPHAKVLSVFGAGG-PIVFGEKSSVGRGGMQAKIRSAQRALAQGVNAVVIASGFKYGILDSIMKGTVVGTLFVPNPSALTPVNSPED-------MAVAARDGSRALLELSSAERSSIVLRLSQLLVDESPRILEANRKDLDAA-SSSLDPQLRSRLKLTHDKLVTLSQGIASVAGQDEPLGRVLSLMEVSSGLVLEQVTAPIGVLLVVFESRPDSLPQIAALALRSGNGLLLKGGKEAKHSNACLHALVQDAIRLETSK--VPPSVVGLVTTRDDVGALLALDHVVDLCIPRGSNSLVSHIKKNTRIPVLGHAEGVCHVYIHDNADLVKATKVAVDAKTDYPAACNALETLLIDEAIVERGAHHA--ILDALVAAGVTLYEGPRAAKLHLVPGALAAPSLSHEYGSLGLTVEV-VAGGVDGAMAHINRYSSGHTECIITEDAAVAETFLNGIDSACVFHNASTRFADGFRFGLGAEVGISTGRIHARGPVGVQGLLTTKWKLRSAKVHTVAAFGQGGTDTYTHKTL 724          
The following BLAST results are available for this feature:
BLAST of EsuBft1017_6a-0001 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JBC7_9PHAE0.000e+0100.00Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=E... [more]
D7G5M6_ECTSI0.000e+093.36Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=E... [more]
A0A835ZBW4_9STRA3.010e-31265.52Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=T... [more]
A0A7S2WAW7_9STRA3.610e-25055.32Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=R... [more]
W7U194_9STRA9.390e-24953.84Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=M... [more]
A0A1W0A8G2_9STRA4.920e-24353.97Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=T... [more]
A0A7S2BH10_9STRA1.320e-24154.89Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=D... [more]
A0A6A3JB98_9STRA3.440e-24054.52Delta-1-pyrroline-5-carboxylate synthase n=24 Tax=... [more]
A0A0N7L745_PLAHL1.100e-23753.95Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=P... [more]
W4H1A3_9STRA7.730e-23553.03Delta-1-pyrroline-5-carboxylate synthase n=10 Tax=... [more]

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BLAST of EsuBft1017_6a-0001 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JBC7_9PHAE0.000e+0100.00Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=E... [more]
D7G5M6_ECTSI0.000e+093.36Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=E... [more]
A0A835ZBW4_9STRA7.110e-30165.52Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=T... [more]
A0A7S2WAW7_9STRA9.050e-24055.32Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=R... [more]
W7U194_9STRA2.230e-23853.84Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=M... [more]
A0A1W0A8G2_9STRA3.160e-23353.97Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=T... [more]
A0A7S2BH10_9STRA2.240e-23154.89Delta-1-pyrroline-5-carboxylate synthase n=2 Tax=D... [more]
A0A6A3JB98_9STRA1.980e-23054.52Delta-1-pyrroline-5-carboxylate synthase n=24 Tax=... [more]
A0A0N7L745_PLAHL1.560e-22753.95Delta-1-pyrroline-5-carboxylate synthase n=1 Tax=P... [more]
W4H1A3_9STRA3.370e-22553.03Delta-1-pyrroline-5-carboxylate synthase n=10 Tax=... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001057Glutamate/acetylglutamate kinasePRINTSPR00474GLU5KINASEcoord: 338..358
score: 45.33
coord: 244..265
score: 46.13
coord: 281..308
score: 53.44
coord: 204..232
score: 48.15
coord: 152..166
score: 66.42
IPR001048Aspartate/glutamate/uridylate kinasePFAMPF00696AA_kinasecoord: 116..364
e-value: 3.4E-35
score: 121.8
IPR005766Delta l-pyrroline-5-carboxylate synthetaseTIGRFAMTIGR01092TIGR01092coord: 108..822
e-value: 1.1E-247
score: 821.8
IPR005766Delta l-pyrroline-5-carboxylate synthetasePIRSFPIRSF036429P5C_synthetasecoord: 81..841
e-value: 1.2E-266
score: 884.3
IPR016162Aldehyde dehydrogenase, N-terminalGENE3D3.40.605.10Aldehyde Dehydrogenase; Chain A, domain 1coord: 412..818
e-value: 8.1E-143
score: 478.0
IPR015590Aldehyde dehydrogenase domainPFAMPF00171Aldedhcoord: 406..684
e-value: 3.8E-8
score: 32.3
IPR016163Aldehyde dehydrogenase, C-terminalGENE3D3.40.309.10Aldehyde Dehydrogenase; Chain A, domain 2coord: 626..779
e-value: 8.1E-143
score: 478.0
IPR000965GPR domainTIGRFAMTIGR00407TIGR00407coord: 413..811
e-value: 1.6E-125
score: 417.0
IPR000965GPR domainHAMAPMF_00412ProAcoord: 404..821
score: 32.510391
IPR000965GPR domainCDDcd07079ALDH_F18-19_ProA-GPRcoord: 406..815
e-value: 0.0
score: 534.706
IPR036393Acetylglutamate kinase-like superfamilyGENE3D3.40.1160.10coord: 104..390
e-value: 3.4E-73
score: 248.4
IPR036393Acetylglutamate kinase-like superfamilySUPERFAMILY53633Carbamate kinase-likecoord: 116..385
NoneNo IPR availablePIRSRPIRSR016496-1PIRSR016496-1coord: 250..383
e-value: 4.7E-12
score: 43.2
coord: 117..172
e-value: 33.0
score: 1.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 13..46
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 78..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..46
NoneNo IPR availablePANTHERPTHR11063GLUTAMATE SEMIALDEHYDE DEHYDROGENASEcoord: 107..831
IPR019797Glutamate 5-kinase, conserved sitePROSITEPS00902GLUTAMATE_5_KINASEcoord: 336..353
IPR020593Gamma-glutamyl phosphate reductase GPR, conserved sitePROSITEPS01223PROAcoord: 728..749
IPR016161Aldehyde/histidinol dehydrogenaseSUPERFAMILY53720ALDH-likecoord: 405..818

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Scaffold_189contigScaffold_189:518905..538951 -
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.02022-09-29
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Diamond blastp: OGS1.0 vs UniRef902022-09-16
OGS1.0 of Ectocarpus subulatus male Bft15b2020-06-19
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
EsuBft1017_6a-0001EsuBft1017_6a-0001Ectocarpus subulatus male Bft15bmRNAScaffold_189 516605..539627 -


Sequences
The following sequences are available for this feature:

polypeptide sequence

>EsuBft1017_6 ID=EsuBft1017_6|Name=EsuBft1017_6a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=842bp
MSGFPPPSNSLPPVNIGQSASTAGGGGSVDGSSTAGSTTTAGTSTTTVPV
ADMSSIVSGGGELYGNMMVGSLPAMGGGLGESAPPSPELGPVPSANRSGK
SGPVHHSRAALMSATRIVVKAGTSIVSNSRGYPCLSRIGAVVEQCAWLKK
MGKEVILVSSGAVGVGRQLLRKQQLLHTSMGAVLDGHAEVPKGTGRRNYD
SACAAAGQLGLMSLYETLFAQCDVATSQLLVTEFDFRTPERRRHLRYTTS
TMLKLGVVPILNENDAVSGNEGYEADGMFSDNDGLAALVAEQMNAKMLIL
LTDVEGVYNKHPDEEGAKIFHTFDPNRHELMIGEKSAGGRGGMGAKIRAA
SRAVSGGVPSVVIASGLNPYSIERIVCGERVGTMFCANPGLYEDSDDEED
ADDMIVRQAEMARVGGRALCALDSEERAGILRKVAEALLAKSPEITVANE
RDIKAAKANNIAPALLNRLKLTEDKIKVLAEGITSLADGEEPLSKCNSRL
EVQEGLVLKQITSPIGVLLIVFESRPDSLPQIASLAIRSGNGLLLKGGKE
AEHSNACLHRIIVDAVAEGSKGRVPRDCIGLVTSRAAVGELLKLDHLIDL
VIPRGSGSLVNHIKASTKIPVMGHSEGICHVYIDKAADPTKAVEIAVDAK
VNYPSACNAAETILIHKALLEEGAGTCAQVVRALHSTGVTVLGGPRAVDL
GVVGGDKISPGFKTEYGECDLTVSLEIVDSVEAALDHIQMFGSSHTETIV
TEDQDVAKMFLETVDSACVFHNASTRFSDGYRFGLGAEVGVSTGRIHARG
PVGVEGLLTTKWVLRSHGESAHTVGKKDPYLLKPMDLEAGAW
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR001057Glu/AcGlu_kinase
IPR001048Asp/Glu/Uridylate_kinase
IPR005766P5_carboxy_syn
IPR016162Ald_DH_N
IPR015590Aldehyde_DH_dom
IPR016163Ald_DH_C
IPR000965GPR_dom
IPR036393AceGlu_kinase-like_sf
IPR019797Glutamate_5-kinase_CS
IPR020593G-glutamylP_reductase_CS
IPR016161Ald_DH/histidinol_DH