EsuBft124_3a-0001 (mRNA) Ectocarpus subulatus male Bft15b

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	EsuBft124_3a-0001
Unique Name	EsuBft124_3a-0001
Type	mRNA
Organism	Ectocarpus subulatus male Bft15b (Ectocarpus subulatus male Bft15b)

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Scaffold_245	contig	Scaffold_245:438592..443997 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
OGS1.0 of Ectocarpus subulatus male Bft15b	2020-06-19

Properties


Property Name	Value
ProteomeId	EsuBft124_3
Protein domains	TMhelix
Protein domains	CYTOPLASMIC_DOMAIN
Protein domains	NON_CYTOPLASMIC_DOMAIN
Protein domains	TRANSMEMBRANE
Owner	apollo_admin@sb-roscoff.fr
Nb exon	7
Length	231
Hectar predicted targeting category	signal peptide
Ec32 ortholog description	conserved unknown protein
Ec32 ortholog	Ec-15_000830.1
Date last modified	2016-06-14
Date creation	2016-06-14

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a	EsuBft124_3a	Ectocarpus subulatus male Bft15b	gene	Scaffold_245 438592..443997 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a-0001	EsuBft124_3	Ectocarpus subulatus male Bft15b	polypeptide	Scaffold_245 438719..442543 +

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a-0001-exon	agat-exon-94809	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 438592..438759 +
EsuBft124_3a-0001-exon	EsuBft124_3a-0001-exon	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 438973..439017 +
EsuBft124_3a-0001-exon	agat-exon-94808	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 439275..439368 +
EsuBft124_3a-0001-exon	agat-exon-94810	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 440333..440415 +
EsuBft124_3a-0001-exon	agat-exon-94811	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 441546..441711 +
EsuBft124_3a-0001-exon	agat-exon-94807	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 441888..442064 +
EsuBft124_3a-0001-exon	agat-exon-94806	Ectocarpus subulatus male Bft15b	exon	Scaffold_245 442418..443997 +

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a-0001-exon	agat-five_prime_utr-1576	Ectocarpus subulatus male Bft15b	five_prime_UTR	Scaffold_245 438592..438718 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a-0001-cds	EsuBft124_3a-0001-cds	Ectocarpus subulatus male Bft15b	CDS	Scaffold_245 438719..438759 +

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
EsuBft124_3a-0001-exon	agat-three_prime_utr-2371	Ectocarpus subulatus male Bft15b	three_prime_UTR	Scaffold_245 442544..443997 +

Sequences

The following sequences are available for this feature:

mRNA - spliced transcript sequence
protein sequence
mRNA from alignment at Scaffold_245:438592..443997+
coding sequence from alignment at Scaffold_245:438592..443997+

spliced messenger RNA

>EsuBft124_3a-0001 ID=EsuBft124_3a-0001|Name=EsuBft124_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=2313bp|location=Sequence derived from alignment at Scaffold_245:438592..443997+ (Ectocarpus subulatus male Bft15b)|Notes=Excludes all bases but those of type(s): exon.  
TAAGCGACCATCGGAAGTGCAAGGGAAACCACCACCACCATCACCCAGGC
AGCAACCACCGCCGCCGAAGCCCGTTCAGGAAAAGCCGAAGTAACCAGCG
CCTAGGGAGAGCAAGAGTTACTGCACCATGGGGGGTTACGAAGGGCAGAA
CAAGCGCGCGCTCATCTGGGCTGCTTTGTACTTCTTGGCATGGATTCTGC
AAAGATTGTGGTGCCACCTGGGCCTGCCCGACCACTGCGACCTCGCGATG
CGGCCATTGGACGTGCTGACGGATTTGGTGGTGCTCTTCACCTTCTCCCT
CGCACAGGCATACTGGTGTGAGATCTTGCTAGACGACCGTTTCGAGGAGC
TCAAGAAGGCTCCTTGGATTTTCGGCGTCATTCTCCTCACGCCAATTATC
ATCTCGGCCCTTGGGCGTATCGATGGACTCGAGAGGTGGGACAAGGACAA
CGAGGGCCGAGACTTCGAGTTCTCCGCCCTCCGATCGACCATCTTCGTCT
TCGGCCTTATTGCCGCCGTGCTCATTGTAGCGTGGCACTTCGCACGGGCG
TGGCTGACTCTTCCCGGCATGCAGCTGGTGTCGGGAGGCGTTAAGGTCGA
GCCGCCTAAGGAAGACCTTCACGGCATCTACGCCTCCAGCCGCATCGTCA
TCATCGCGTGGTGCATCCTCTTCACTTCGTTCATCATCTCCGAGGACGAC
GGCTTCGATTACAGAGGCGCGCTACTCGCATGGATGCTGTCCCTGATTGC
GGTATTCCCGCACCCCGTGAGTTACGCCTGGCTCGGTGTCAACACGGGTA
TCTTCTTGCAAGGGATCGGTGCCAACCGTCTCCGGTTCCTGGGGTGCAAG
GAATGGTGAACGCGTGATAATCGTCTTACGACTGTTGTTCTTTTGTGTGC
GGGGGAGACAAAGAAGTGCATCGCGGAGCGGACTTAGTCGCCCGCGATGT
CGTCTGAGGCTTCGCGATATCAGTGGCGTACTGTGAAATTTTCTGCTGGC
TAGTGTGAGCTAGGGTCATGATGATGGTGTGGGGAAGGTTACGCTTGCGC
AGCTGCTACACTTTTCGTTTGGTCGAGAGTTTTTGGCTGAGGGATCATGG
GGCCCATAACAGGTTGGCGTTACACCGATACCCGCTCACACGACGAATTG
TGTGAACTGACAGTTTCGTTGTGAGTCGGTAGGGTTTCGGCGGTTTGTTT
GGGCGCTACCTTGTTGGATTATTCCAACTGTTCGGTGCCACATATTTTCT
TTGATTTCGGTTCTTCGTTGAGGTACCCACTCCGAATCTTCACCGATATG
GTCGGGTCCGATTTATTCATGATTTTCGCTTGATTGTCGTTTTTGTGTCT
GTATTCGATTTGACAGTGAACGGGCAGGCAGCGTGCGCTTCGTTGGACTT
GGGTTGAGGGAGGTTGGGCCTCTGCGTTGTTTGTTCGTGAGAAAGCACCA
AGCATCCATGAACGAATCATGCCGTAGGGGCGTTGGCGGGTTTTACTGAT
ACCACGCCACACATCATCGTGAGTGTGAGCCGATGGTCCGCTCCTCACGA
TGAGAAACGCCTCCCAGGAAAAGAATAATATCTCACGCGTCGTAGCTACA
ATCAGTCCGAATTTCAGACCGCCGAGAGCGTCTCGGAGTTTATATGTTGA
TTTTGGCGGGGAGGATATTACAAAGAGGATCTGAGAGTACAGCAGTACCA
ACGTTGGTTGCGCGGGCTTGGGGGTGGGGTGAATGAAGTGAAAGTGTCTT
GTTTCTGGGAACAATAATAGTAGTGTTGTGGTGTCTGTCCCAGAATTCAA
GGAAGTTGAAGCGTGGTAAGATGTTAATTTGACTTCTTGGTGCTTGTATT
TATAATTTCGTTCCGGGGAGGACCAGACGGGTTCTCTGCTCAATCAAGGT
GCTGCCATTTATTTGCAGGCGCGAAGACGTGACCGACAGCTAGCTGTAGA
TGTCACGGCCTCTTTCGACATCTTTCTCCGGTGTGATCAGTGTAGTCTGG
CGTGTGGTACCATAGAACAACGGTTACGCTAGTACGCACGCGGTGGCGAC
GCAGCATGGCACGGTATGCGGATGTGGCGGTTAGATGACCTGACTCTGGC
GATTCGAGCCATCCTTTTGAAAAGGATGGCCATTTTATTACACGTGCATG
CAAGTTAATCTACTTGCATCGTTGCGTTCCCTGTCGGTGCCGCCTTCTTT
CGTGCTGTTTTTCCGCTTTTGTTTCTTGAGAGGGAAATTCTTTCTTATCT
GAAGGAGGTTGTAGGGGTCTTATCATGGATGTGGCGTTGCATCAAGGTGC
TGCCATTTATTTG

protein sequence of EsuBft124_3a-0001

>EsuBft124_3 ID=EsuBft124_3|Name=EsuBft124_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=polypeptide|length=243bp
MGGYEGQNKRALIWAALYFLAWILQRLWCHLGLPDHCDLAMRPLDVLTDL
VVLFTFSLAQAYWCEILLDDRFEELKKAPWIFGVILLTPIIISALGRIDG
LERWDKDNEGRDFEFSALRSTIFVFGLIAAVLIVAWHFARAWLTLPGMQL
VSGGVKVEPPKEDLHGIYASSRIVIIAWCILFTSFIISEDDGFDYRGALL
AWMLSLIAVFPHPVSYAWLGVNTGIFLQGIGANRLRFLGCKEW

mRNA from alignment at Scaffold_245:438592..443997+

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

>EsuBft124_3a-0001 ID=EsuBft124_3a-0001|Name=EsuBft124_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=mRNA|length=5406bp|location=Sequence derived from alignment at Scaffold_245:438592..443997+ (Ectocarpus subulatus male Bft15b)
TAAGCGACCATCGGAAGTGCAAGGGAAACCACCACCACCATCACCCAGGC
AGCAACCACCGCCGCCGAAGCCCGTTCAGGAAAAGCCGAAGTAACCAGCG
CCTAGGGAGAGCAAGAGTTACTGCACCATGGGGGGTTACGAAGGGCAGAA
CAAGCGCGCGCTCATCTGGTGAGAAGGGGCGTGTGTTTCGCGCCGTATTC
ATTTTCCGACGCTTGTCGCCCTGGAAGGGACCATGGCGTGTGCTCCGGCC
CATGCTGCTGCCGTTGACGGCTGTGCATGCGTGCGGCAACGTTGTGTGCG
ATAGCGTTCCTTGCACCAACCCTCTCTCGTCCCTCTCTCGCACTTGCCGT
TTGTCAAAACCTCTTGGCTCTGTTGCGACAGGGCTGCTTTGTACTTCTTG
GCATGGATTCTGCAAAGATTGTGGTGGTGAGTGCTCAGCCTGCTGCGTGC
TTGGGCGCCATCCACCACTTGGGTCCTGCTCGTAGCAAGGTAGTAGGCCC
CGTAACTAGTCCTAGTATGCTCTACCCTTTTTCGCCCGAGGCGAGCGCTG
ATGCCGGACGCAGATTTTGCTGGTCGCGCAATTTTTTTCCTGTTGTGACA
AAATTGGTCAACGGCCAAAAAATGTCCTGAACGCTTGCGCCTGACACCTG
GTGCCGCTGCATCTGCGTGTCCATCTCCTACAGCCACCTGGGCCTGCCCG
ACCACTGCGACCTCGCGATGCGGCCATTGGACGTGCTGACGGATTTGGTG
GTGCTCTTCACCTTCTCCCTCGCACAGGTAGGGATGGAGGTCCATCTGCG
TGGTAGAGTACAGATGTAGTGGTTGGCAGGATCACCTCCTGACAGCATGC
ATATCGATCCGTGCACATTCTGTCTTGGGAGCAACCGTGGAACACACACG
CACGCACCCTTGTCGCGCAACGGTCAGCGATTTTTGGGATGGATAGCTGT
ACTGCTGTTGTTCATTTACCCTCGGTCTTCCGCGTCATGACAAGAGAAAC
CTTTTTGGCAGTAGCATCGGTGTTCCATCGGCACAAGGGAACAATATTAA
AGTCTAGGCAAAACAGACCGCAGTTCCCGCCCTGTTCCTGTTCCTGCGAC
TCATTGTGCTCGCCAAAAATGCGCAGCAGCGGCATCATCATCATCATCTG
CAGCTAGAAGCTGCCATTGTTCAATGGTGGCTTGGCACCTGGCAATGCCG
CTTTCTCCGGCCTTCCTTTCATGCATTCGTCGAATGGCCGTTCGTGATTC
CGACATCCGTGCTTCGCACTATTAGACTAGGTGGAAAAAGCGGGATTTCT
TTCGTCGCGCTCTCTATCATCCAGAATGTGGGAAGGGTTGAACGGCTGTC
CCATGCATGTTGAGAAGCTGAAGTGGACGTAGCATATGATAGGGTCACAT
CCGAAAACATGCCCATGCTAGCAGATGCCCACGCTGGCAGGCGGCTAATG
TGTAGTGCATTTCCTCGTGCCCCAAGCGGTAATCTCGTGCGTCAGTAGTG
CCAAGCCGAGACAATACATGACGTGCAAATCAGCAAATATGTTATTCCGT
CGTCCTTGAGGGTACGTATCGCTGCCTACTTGGTCGCGGTCGACATTTTT
TGTTCGAAAATATACGCAATCTCACAGGAAGACTGCCCGTTCGAATTAAA
CCCTGCTCCATCTACACGCGCATCATACCCACTGCTGTTTTCCCATGCTC
CTCGCTCGCATTTTATTTTTATGGTGGTCCTGCTGATGCAGGCATACTGG
TGTGAGATCTTGCTAGACGACCGTTTCGAGGAGCTCAAGAAGGCTCCTTG
GATTTTCGGCGTCATTCTCCTCACGTGAGTGCCTCCACACACACCATGAT
ATGTGCACCCTTTGTTGCTGTGTGTTGTGTGTGTGTGTGTGCGTGTGCTT
TGTCTTCACTTCAAATCAGCGCTCACAGCACTATTATAGTGCTGTTTTCT
CCAACCCGTGGACACACAGAAGAGCCCCTCTTTACCCTCCCCTCCTGCGG
TACTTTGCCTCCGATTATTAGTGCGAAAAGAGCACATCACTCTCTTCCCT
CTACGTACACACGACCTAGCGCCATCCACTAGGCCAGGAGCGGAAGCGAG
TGAAAAACTCTCCGAAAGCTCGCTTACGCCGGGGTTCGAACCCCTGACCT
GACCTCTGAAAGGTTTCGAGGTTACCAAGTAGACCACTGGGGCGACCGGT
GTGTTAAGTCATCTCGGAACAGCATTGTTATCGCACAGCAAGTTTCCACT
TTTTATTTCAGCCTCCTGTTTTCGAGTTAGGGCTAGCGTCAACAAACACT
ACGAAAAATACGTGCACTCTTCCTTCATGTTGGGTGGTTTCGAACTACAG
ATGTATGCTTTCCTCTATACGACTTGGGGCTCAACACACACCGTACAAGT
GCACGCTTCAATCATGTTGTCGATGCCCATAGCACGGTTAATCTTTTTGA
TTTGAGAGTTTTTCGGGATTGACGCGAGGTACGAGGGATGTTGGTATGTA
CCTACACCGACTACAGTAGTCCTACCGGCGACCCCTCGAGCTTCTAATAA
ATAGTACTAGCAGTAGTCCAGTTTGTAGATGTGCCGTCCCAGAAATACCA
CAGTAGTCGAAGCTACGGGTGTACAGGTGTCAACGCGAGGAGGGAATGGG
TTGCCACAAGCCAAGGGTCGGACTTGCTGTATATGATTGGTCTGTTGGCC
TTCATGAACAACCATAAAACAAGGAAGTCACCGTACACATTTGTGGTGTC
TGTTGCGACCCTAAAAAGGCGAGACGGAAACACACACAAAAGCACAACAA
ACCGCGAGGGGCTTCCGCTCGACGTCGTGTTCCTTGGCACTTTGCGGTGT
TCCTTGGCACCATGCGGTGACTTTCTCTCCCCATCTCATGCTCCTCTCCG
TCACTACTCTGACCTGCCACGCTTCTAAACCTCGTGATGCTCATAACAAC
GCAGGCCAATTATCATCTCGGCCCTTGGGCGTATCGATGGACTCGAGAGG
TGGGACAAGGACAACGAGGGCCGAGACTTCGAGTTCTCCGCCCTCCGATC
GACCATCTTCGTCTTCGGCCTTATTGCCGCCGTGCTCATTGTAGCGTGGC
ACTTCGCACGGGCGTGGCTGGTGAGAGAAACACACCGATATTTTTTGCCG
GACAAGCGGACATGTGTTGTACCCCAGCAACAATCGCATCGAAGCTTGCA
CGATATGTCAACCCGACCGACTCGTTACGTCTAAGCGCCCAACGTTTCCC
CTCGCTTCCTCCCCCGTGGTTCTTGTTTACTTATTTTTTTCGGAAGACTC
TTCCCGGCATGCAGCTGGTGTCGGGAGGCGTTAAGGTCGAGCCGCCTAAG
GAAGACCTTCACGGCATCTACGCCTCCAGCCGCATCGTCATCATCGCGTG
GTGCATCCTCTTCACTTCGTTCATCATCTCCGAGGACGACGGCTTCGATT
ACAGAGGCGCGCTACTCGCATGGGTGCGTACATGTGGCGGGAACTTTTGC
TTTTCGGTTGGGTACAATCTTGTTGGTTAGATCATGCGTGTGGCTCGGTA
TCGAGTTGGGTATGTTGGTGAGGCTTCGTATGTAGTACCTTGTTCAAGGT
GCATTGCTGTAGAACCTCGTGTTGGGCTGGCGAAAAAAGGAGGCTCTCTC
TTTGTTCTGGTGTACCTTCTATTTCCAATTAAGTACTAGTGGATGGCGGG
CGATGTACAGGAGAAGGTTGTTCTAGTAGCAGCAGCAGTGTTGATTGGTG
GACAGTGACCCACCCCCACGCTCTTACAAGCTCGAACCCCTCCTCCCTTT
TCCCCGCCGCGCCGTGCGATCCGCAGATGCTGTCCCTGATTGCGGTATTC
CCGCACCCCGTGAGTTACGCCTGGCTCGGTGTCAACACGGGTATCTTCTT
GCAAGGGATCGGTGCCAACCGTCTCCGGTTCCTGGGGTGCAAGGAATGGT
GAACGCGTGATAATCGTCTTACGACTGTTGTTCTTTTGTGTGCGGGGGAG
ACAAAGAAGTGCATCGCGGAGCGGACTTAGTCGCCCGCGATGTCGTCTGA
GGCTTCGCGATATCAGTGGCGTACTGTGAAATTTTCTGCTGGCTAGTGTG
AGCTAGGGTCATGATGATGGTGTGGGGAAGGTTACGCTTGCGCAGCTGCT
ACACTTTTCGTTTGGTCGAGAGTTTTTGGCTGAGGGATCATGGGGCCCAT
AACAGGTTGGCGTTACACCGATACCCGCTCACACGACGAATTGTGTGAAC
TGACAGTTTCGTTGTGAGTCGGTAGGGTTTCGGCGGTTTGTTTGGGCGCT
ACCTTGTTGGATTATTCCAACTGTTCGGTGCCACATATTTTCTTTGATTT
CGGTTCTTCGTTGAGGTACCCACTCCGAATCTTCACCGATATGGTCGGGT
CCGATTTATTCATGATTTTCGCTTGATTGTCGTTTTTGTGTCTGTATTCG
ATTTGACAGTGAACGGGCAGGCAGCGTGCGCTTCGTTGGACTTGGGTTGA
GGGAGGTTGGGCCTCTGCGTTGTTTGTTCGTGAGAAAGCACCAAGCATCC
ATGAACGAATCATGCCGTAGGGGCGTTGGCGGGTTTTACTGATACCACGC
CACACATCATCGTGAGTGTGAGCCGATGGTCCGCTCCTCACGATGAGAAA
CGCCTCCCAGGAAAAGAATAATATCTCACGCGTCGTAGCTACAATCAGTC
CGAATTTCAGACCGCCGAGAGCGTCTCGGAGTTTATATGTTGATTTTGGC
GGGGAGGATATTACAAAGAGGATCTGAGAGTACAGCAGTACCAACGTTGG
TTGCGCGGGCTTGGGGGTGGGGTGAATGAAGTGAAAGTGTCTTGTTTCTG
GGAACAATAATAGTAGTGTTGTGGTGTCTGTCCCAGAATTCAAGGAAGTT
GAAGCGTGGTAAGATGTTAATTTGACTTCTTGGTGCTTGTATTTATAATT
TCGTTCCGGGGAGGACCAGACGGGTTCTCTGCTCAATCAAGGTGCTGCCA
TTTATTTGCAGGCGCGAAGACGTGACCGACAGCTAGCTGTAGATGTCACG
GCCTCTTTCGACATCTTTCTCCGGTGTGATCAGTGTAGTCTGGCGTGTGG
TACCATAGAACAACGGTTACGCTAGTACGCACGCGGTGGCGACGCAGCAT
GGCACGGTATGCGGATGTGGCGGTTAGATGACCTGACTCTGGCGATTCGA
GCCATCCTTTTGAAAAGGATGGCCATTTTATTACACGTGCATGCAAGTTA
ATCTACTTGCATCGTTGCGTTCCCTGTCGGTGCCGCCTTCTTTCGTGCTG
TTTTTCCGCTTTTGTTTCTTGAGAGGGAAATTCTTTCTTATCTGAAGGAG
GTTGTAGGGGTCTTATCATGGATGTGGCGTTGCATCAAGGTGCTGCCATT
TATTTG

Coding sequence (CDS) from alignment at Scaffold_245:438592..443997+

>EsuBft124_3a-0001 ID=EsuBft124_3a-0001|Name=EsuBft124_3a-0001|organism=Ectocarpus subulatus male Bft15b|type=CDS|length=732bp|location=Sequence derived from alignment at Scaffold_245:438592..443997+ (Ectocarpus subulatus male Bft15b)
ATGGGGGGTTACGAAGGGCAGAACAAGCGCGCGCTCATCTGGGCTGCTTT
GTACTTCTTGGCATGGATTCTGCAAAGATTGTGGTGCCACCTGGGCCTGC
CCGACCACTGCGACCTCGCGATGCGGCCATTGGACGTGCTGACGGATTTG
GTGGTGCTCTTCACCTTCTCCCTCGCACAGGCATACTGGTGTGAGATCTT
GCTAGACGACCGTTTCGAGGAGCTCAAGAAGGCTCCTTGGATTTTCGGCG
TCATTCTCCTCACGCCAATTATCATCTCGGCCCTTGGGCGTATCGATGGA
CTCGAGAGGTGGGACAAGGACAACGAGGGCCGAGACTTCGAGTTCTCCGC
CCTCCGATCGACCATCTTCGTCTTCGGCCTTATTGCCGCCGTGCTCATTG
TAGCGTGGCACTTCGCACGGGCGTGGCTGACTCTTCCCGGCATGCAGCTG
GTGTCGGGAGGCGTTAAGGTCGAGCCGCCTAAGGAAGACCTTCACGGCAT
CTACGCCTCCAGCCGCATCGTCATCATCGCGTGGTGCATCCTCTTCACTT
CGTTCATCATCTCCGAGGACGACGGCTTCGATTACAGAGGCGCGCTACTC
GCATGGATGCTGTCCCTGATTGCGGTATTCCCGCACCCCGTGAGTTACGC
CTGGCTCGGTGTCAACACGGGTATCTTCTTGCAAGGGATCGGTGCCAACC
GTCTCCGGTTCCTGGGGTGCAAGGAATGGTGA