mRNA_Ecto-sp9_F_contig9341.17501.1 (mRNA) Ectocarpus species9 EcSCA_722f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp9_F_contig9341.17501.1
Unique NamemRNA_Ecto-sp9_F_contig9341.17501.1
TypemRNA
OrganismEctocarpus species9 EcSCA_722f female (Ectocarpus species9 EcSCA_722f female)
Homology
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0R1_ECTSI)

HSP 1 Score: 344 bits (883), Expect = 1.310e-109
Identity = 206/219 (94.06%), Postives = 207/219 (94.52%), Query Frame = 1 
Query:    1 MHRAGEPEEWGTATCGTGSGGAGSAARGPSVFRGWRSSSKAASMSSSXXXXXXXDPGAGDASSPSPFSRGRPFRGSFLPSSATGASPSSTGLGAAVGGDAVSSSAXXXXXXXXXXXXXXXXXXXAPWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            MH+AGEPEEWGTATCGTGSGGAGSAARGPSVFRGWRSSSKAASMSSS       DPGA DASSPSPFSRGRPFRGSFLPSSATGASPSSTGLGAAVGGDA SSSA  XXXXXXXXXXXXXXXX APWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV
Sbjct:    1 MHQAGEPEEWGTATCGTGSGGAGSAARGPSVFRGWRSSSKAASMSSSTAGAGGGDPGAEDASSPSPFSRGRPFRGSFLPSSATGASPSSTGLGAAVGGDAASSSAETXXXXXXXXXXXXXXXXGAPWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 219
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JCY0_9PHAE)

HSP 1 Score: 311 bits (798), Expect = 4.580e-95
Identity = 196/209 (93.78%), Postives = 197/209 (94.26%), Query Frame = 1 
Query:   31 GTATCGTGSGGAGSAARGPSVFRGWRSSSKAASMSSSXXXXXXXDPGAGDASSPSPFSRGRPFRGSFLPSSATGASPSSTGLGAAVGGDAVSSSAXXXXXXXXXXXXXXXXXXXAPWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            GTATCGTGSGG GSAARGPSVFRGWRSS KAASMSSS       DPGAGDASSPS FSRGRPFRGSFLPSSATGA+PSSTGLGAAVGGDA SSS XXXXXXXXXXXXXXXXXXXAPWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV
Sbjct:  106 GTATCGTGSGGVGSAARGPSVFRGWRSS-KAASMSSSTAGAGGGDPGAGDASSPSLFSRGRPFRGSFLPSSATGATPSSTGLGAAVGGDAASSSXXXXXXXXXXXXXXXXXXXXAPWLARGLKALVRGGRGGETGTQLPSLGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 313
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Stromal (processing peptidase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C760_9STRA)

HSP 1 Score: 68.6 bits (166), Expect = 8.290e-10
Identity = 31/41 (75.61%), Postives = 35/41 (85.37%), Query Frame = 1 
Query:  535 VMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            V+ + LP+HP MR G+LPNGLRYIIL N SPPERFEAHLEV
Sbjct:   12 VLRAALPSHPLMRSGVLPNGLRYIILPNASPPERFEAHLEV 52
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=7 Tax=Phaseoleae TaxID=163735 RepID=V7CFZ1_PHAVU)

HSP 1 Score: 67.8 bits (164), Expect = 1.550e-9
Identity = 31/56 (55.36%), Postives = 41/56 (73.21%), Query Frame = 1 
Query:  490 QDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            QD  V      +++G +SSELP+HP++  G L NGLRY+IL N+ PP+RFEAHLEV
Sbjct:  157 QDSSVYDNELERIEGFLSSELPSHPKLHRGQLKNGLRYLILPNKVPPKRFEAHLEV 212
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Putative (zinc protease PqqL n=1 Tax=Morella rubra TaxID=262757 RepID=A0A6A1UWN4_9ROSI)

HSP 1 Score: 67.8 bits (164), Expect = 1.550e-9
Identity = 28/47 (59.57%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:  517 RSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            R++++G +SSELP+HP++  G L NGLRY+IL N+ PP RFEAH+EV
Sbjct:  181 RTELEGFLSSELPSHPKLHRGQLKNGLRYLILPNKVPPNRFEAHMEV 227
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=1 Tax=Vitis vinifera TaxID=29760 RepID=A5APK5_VITVI)

HSP 1 Score: 67.4 bits (163), Expect = 2.080e-9
Identity = 28/48 (58.33%), Postives = 39/48 (81.25%), Query Frame = 1 
Query:  514 GRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            GR++++G + SELP+HP++  G L NGLRY+IL N+ PP RFEAH+EV
Sbjct:  323 GRAELEGFLCSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEV 370
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=4 Tax=Vitis TaxID=3603 RepID=D7UDI7_VITVI)

HSP 1 Score: 67.4 bits (163), Expect = 2.100e-9
Identity = 28/48 (58.33%), Postives = 39/48 (81.25%), Query Frame = 1 
Query:  514 GRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            GR++++G + SELP+HP++  G L NGLRY+IL N+ PP RFEAH+EV
Sbjct:  188 GRAELEGFLCSELPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEV 235
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=6 Tax=Prunus TaxID=3754 RepID=A0A6J5Y060_PRUAR)

HSP 1 Score: 67.4 bits (163), Expect = 2.120e-9
Identity = 27/48 (56.25%), Postives = 40/48 (83.33%), Query Frame = 1 
Query:  514 GRSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            G+++++G +SSE+P+HP++  G L NGLRY+IL N+ PP RFEAH+EV
Sbjct:  181 GKTELEGFLSSEIPSHPKLYRGQLKNGLRYLILPNKVPPNRFEAHMEV 228
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: stromal (processing peptidase, chloroplastic-like n=1 Tax=Ananas comosus TaxID=4615 RepID=A0A6P5GV35_ANACO)

HSP 1 Score: 66.2 bits (160), Expect = 2.600e-9
Identity = 28/46 (60.87%), Postives = 37/46 (80.43%), Query Frame = 1 
Query:  520 SKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            +++ G +SS LPTHP++  G L NGLRYIIL N++PP RFEAH+EV
Sbjct:  185 TELDGFLSSPLPTHPKLHRGQLKNGLRYIILPNKTPPNRFEAHMEV 230
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BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Match: Uncharacterized (protein n=1 Tax=Malus domestica TaxID=3750 RepID=A0A498IFC1_MALDO)

HSP 1 Score: 67.0 bits (162), Expect = 2.830e-9
Identity = 27/47 (57.45%), Postives = 39/47 (82.98%), Query Frame = 1 
Query:  517 RSKMQGVMSSELPTHPQMREGMLPNGLRYIILNNQSPPERFEAHLEV 657
            ++K++G +SSE+P+HP++  G L NGLRY+IL N+ PP RFEAH+EV
Sbjct:  127 KTKLEGFLSSEIPSHPKLYRGQLKNGLRYVILPNKVPPNRFEAHMEV 173
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp9_F_contig9341.17501.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Uncharacterized1.310e-10994.06protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Uncharacterized4.580e-9593.78protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=... [more]
Stromal8.290e-1075.61processing peptidase n=1 Tax=Tribonema minus TaxID... [more]
Uncharacterized1.550e-955.36protein n=7 Tax=Phaseoleae TaxID=163735 RepID=V7CF... [more]
Putative1.550e-959.57zinc protease PqqL n=1 Tax=Morella rubra TaxID=262... [more]
Uncharacterized2.080e-958.33protein n=1 Tax=Vitis vinifera TaxID=29760 RepID=A... [more]
Uncharacterized2.100e-958.33protein n=4 Tax=Vitis TaxID=3603 RepID=D7UDI7_VITV... [more]
Uncharacterized2.120e-956.25protein n=6 Tax=Prunus TaxID=3754 RepID=A0A6J5Y060... [more]
stromal2.600e-960.87processing peptidase, chloroplastic-like n=1 Tax=A... [more]
Uncharacterized2.830e-957.45protein n=1 Tax=Malus domestica TaxID=3750 RepID=A... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp9_F_contig9341contigEcto-sp9_F_contig9341:3230..5086 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus species9 EcSCA_722f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score345.9
Seed ortholog evalue1.4e-92
Seed eggNOG ortholog2880.D7G0R1
GOsGO:0000003,GO:0003006,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005829,GO:0007275,GO:0008150,GO:0009507,GO:0009532,GO:0009536,GO:0009570,GO:0009790,GO:0009791,GO:0009793,GO:0010154,GO:0022414,GO:0032501,GO:0032502,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0048316,GO:0048608,GO:0048731,GO:0048856,GO:0061458
EggNOG free text desc.Belongs to the peptidase M16 family
EggNOG OGsCOG1025@1,KOG0959@2759
Ec32 ortholog descriptionPeptidase M16, C-terminal domain
Ec32 orthologEc-20_001370.5
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons3
Model size657
Cds size657
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp9_F_contig9341.17501.1prot_Ecto-sp9_F_contig9341.17501.1Ectocarpus species9 EcSCA_722f femalepolypeptideEcto-sp9_F_contig9341 3230..5086 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622931013.280483-CDS-Ecto-sp9_F_contig9341:3229..32581622931013.280483-CDS-Ecto-sp9_F_contig9341:3229..3258Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 3230..3258 +
1692278992.8503573-CDS-Ecto-sp9_F_contig9341:3229..32581692278992.8503573-CDS-Ecto-sp9_F_contig9341:3229..3258Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 3230..3258 +
1622931013.2936418-CDS-Ecto-sp9_F_contig9341:4070..43991622931013.2936418-CDS-Ecto-sp9_F_contig9341:4070..4399Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 4071..4399 +
1692278992.8642304-CDS-Ecto-sp9_F_contig9341:4070..43991692278992.8642304-CDS-Ecto-sp9_F_contig9341:4070..4399Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 4071..4399 +
1622931013.317555-CDS-Ecto-sp9_F_contig9341:4787..50861622931013.317555-CDS-Ecto-sp9_F_contig9341:4787..5086Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 4788..5086 +
1692278992.8734028-CDS-Ecto-sp9_F_contig9341:4787..50861692278992.8734028-CDS-Ecto-sp9_F_contig9341:4787..5086Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig9341 4788..5086 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp9_F_contig9341.17501.1

>prot_Ecto-sp9_F_contig9341.17501.1 ID=prot_Ecto-sp9_F_contig9341.17501.1|Name=mRNA_Ecto-sp9_F_contig9341.17501.1|organism=Ectocarpus species9 EcSCA_722f female|type=polypeptide|length=219bp
MHRAGEPEEWGTATCGTGSGGAGSAARGPSVFRGWRSSSKAASMSSSTAG
AGGGDPGAGDASSPSPFSRGRPFRGSFLPSSATGASPSSTGLGAAVGGDA
VSSSAEAGAGGGGAGGLGKGIGAGAPWLARGLKALVRGGRGGETGTQLPS
LGAVMEAVDARGAQDEVVAAEGRSKMQGVMSSELPTHPQMREGMLPNGLR
YIILNNQSPPERFEAHLEV
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mRNA from alignment at Ecto-sp9_F_contig9341:3230..5086+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp9_F_contig9341.17501.1 ID=mRNA_Ecto-sp9_F_contig9341.17501.1|Name=mRNA_Ecto-sp9_F_contig9341.17501.1|organism=Ectocarpus species9 EcSCA_722f female|type=mRNA|length=1857bp|location=Sequence derived from alignment at Ecto-sp9_F_contig9341:3230..5086+ (Ectocarpus species9 EcSCA_722f female)
ATGCATCGAGCGGGGGAGCCGGAGGAATGGTAGGTTTGGCGAGTTTTTTT
CAGGGCACCGCCCGCTCCTGCCTCCGGTGCAAACCTGAAGAATGGTTTAT
CATTGTGTGCTACCTACATGTTGTAGGCATGCCGCCTACGCAAGAGGTTG
GGGAGAATTCCTCCTGTGTCGTGCGCTAATAGCAAGCCTCTCCCTATGTT
AGAGGAATACTCTCACATCGCGGTGTATGCGGATATCTCTAAAGAGCTGC
GGCAACTTGTAAAAGGTCTCGGTGGACCCAGCTCGGTGGACCCAATTATA
AGAAGGAGAGAGTCCCGTGTGTTGTGTTTTCGTTCGGGTTTACTCTCGCG
GCCTCTAACTGCTGTGTCGAGTGTGGCGTGACATCCCGCCGACGTGTCGT
GTGTCGTGCCTTTCGGACGGGATAAGACCCTAGTTTGGACGCGCATGGTT
TTTGGAGATACATAAGAGGCACTTGTGCATATGTTGCGTGTGAAAACATG
CCAACAATATAATGCGGCAACGACCACACACAGCCCCCAACAAGAACCAT
GCATGCAATCTTCGCATATCAAGAACGACGCCCGTTAAGGTTATCGTTGT
AGTCATTCTGATCCTCTTCGTATACGGATGCGTCTGCCACCCACCCACCC
ACCCCCGGGCGCGCACGCACGCCCGTCAACTCTCTCTCTACAGCAGGTGA
TCATGCGGCCAGGGCGACACCGTCGTCGGCTACCGCCTTCCAGGTGGCCG
GAAACCCTTGGCTGTCGTCCGCAACCGGAGGCCCGCGAAATCGCGCTTCT
TCCGACCCCTCTCGCGCCTGCAACAACCTTGCCGACAGCAGGGGCACCGC
GACATGCGGCACGGGGTCCGGGGGGGCGGGGTCGGCGGCGAGGGGGCCGA
GCGTGTTCCGGGGATGGCGCTCTTCTTCCAAGGCCGCCTCAATGTCGTCG
TCGACTGCTGGCGCTGGCGGCGGTGACCCTGGAGCAGGGGACGCGTCATC
GCCGTCCCCGTTTTCGCGAGGGAGACCGTTCCGGGGATCGTTCCTCCCGT
CGTCGGCGACTGGGGCTAGTCCTAGCTCCACCGGGCTGGGTGCCGCCGTT
GGCGGCGACGCCGTCAGTTCTTCCGCGGAGGCTGGGGCGGGCGGCGGCGG
GGCGGGGGGCCTAGGGAAAGGTGCTCGCTATTAGTGCTATTTAGTCTGTC
GCATCATGCCGGCAGCAGCCGATTGTTTGGTGTAGATGGATGGCATCCAT
CCCCCACTTTTGTGTCATCGTTTTTACCGAAGTGATACATGCTGGTTATC
TGCGCCGATTACGGACCAACAGCGAGGCCGGTTGGCGTGTTTTGCACGCA
GCAGTCTCCATAATGGACCGTCCATTTTCGGGAGTTTGGAGGTTGGGTAA
CGCCGCTGTAGTTGGCACTTTTGAGAGCCGTCGTTACAATGGCTGCACGA
AAGGGTTGGAGTTCCCACCCCCATGCTGCTCTGTTCGCACTCACCTTTCA
GTACTCCTACTTTTTCGAGACAAAAACTGTCGACTCTTGTTGTTATTATT
ATTTGCAGGGATCGGTGCGGGGGCGCCGTGGCTTGCGCGGGGGCTCAAGG
CCCTCGTGAGGGGGGGAAGGGGGGGAGAGACGGGGACCCAGCTCCCGTCG
CTGGGAGCCGTCATGGAAGCGGTGGACGCCCGCGGGGCCCAGGACGAGGT
GGTGGCGGCGGAGGGCAGGAGCAAGATGCAGGGGGTGATGTCCTCGGAGC
TGCCCACGCACCCGCAGATGCGCGAAGGCATGCTGCCCAACGGGCTGCGT
TACATCATCCTGAACAACCAGAGCCCTCCGGAGAGGTTCGAGGCGCATCT
GGAGGTG
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Coding sequence (CDS) from alignment at Ecto-sp9_F_contig9341:3230..5086+

>mRNA_Ecto-sp9_F_contig9341.17501.1 ID=mRNA_Ecto-sp9_F_contig9341.17501.1|Name=mRNA_Ecto-sp9_F_contig9341.17501.1|organism=Ectocarpus species9 EcSCA_722f female|type=CDS|length=1314bp|location=Sequence derived from alignment at Ecto-sp9_F_contig9341:3230..5086+ (Ectocarpus species9 EcSCA_722f female)
ATGCATCGAGCGGGGGAGCCGGAGGAATGATGCATCGAGCGGGGGAGCCG
GAGGAATGGGGCACCGCGACATGCGGCACGGGGTCCGGGGGGGCGGGGTC
GGCGGCGAGGGGGCCGAGCGTGTTCCGGGGATGGCGCTCTTCTTCCAAGG
CCGCCTCAATGTCGTCGTCGACTGCTGGCGCTGGCGGCGGTGACCCTGGA
GCAGGGGACGCGTCATCGCCGTCCCCGTTTTCGCGAGGGAGACCGTTCCG
GGGATCGTTCCTCCCGTCGTCGGCGACTGGGGCTAGTCCTAGCTCCACCG
GGCTGGGTGCCGCCGTTGGCGGCGACGCCGTCAGTTCTTCCGCGGAGGCT
GGGGCGGGCGGCGGCGGGGCGGGGGGCCTAGGGAAAGGGGCACCGCGACA
TGCGGCACGGGGTCCGGGGGGGCGGGGTCGGCGGCGAGGGGGCCGAGCGT
GTTCCGGGGATGGCGCTCTTCTTCCAAGGCCGCCTCAATGTCGTCGTCGA
CTGCTGGCGCTGGCGGCGGTGACCCTGGAGCAGGGGACGCGTCATCGCCG
TCCCCGTTTTCGCGAGGGAGACCGTTCCGGGGATCGTTCCTCCCGTCGTC
GGCGACTGGGGCTAGTCCTAGCTCCACCGGGCTGGGTGCCGCCGTTGGCG
GCGACGCCGTCAGTTCTTCCGCGGAGGCTGGGGCGGGCGGCGGCGGGGCG
GGGGGCCTAGGGAAAGGGATCGGTGCGGGGGCGCCGTGGCTTGCGCGGGG
GCTCAAGGCCCTCGTGAGGGGGGGAAGGGGGGGAGAGACGGGGACCCAGC
TCCCGTCGCTGGGAGCCGTCATGGAAGCGGTGGACGCCCGCGGGGCCCAG
GACGAGGTGGTGGCGGCGGAGGGCAGGAGCAAGATGCAGGGGGTGATGTC
CTCGGAGCTGCCCACGCACCCGCAGATGCGCGAAGGCATGCTGCCCAACG
GGCTGCGTTACATCATCCTGAACAACCAGAGCCCTCCGGAGAGGTTCGAG
GCGCATCTGGAGGTGGGATCGGTGCGGGGGCGCCGTGGCTTGCGCGGGGG
CTCAAGGCCCTCGTGAGGGGGGGAAGGGGGGGAGAGACGGGGACCCAGCT
CCCGTCGCTGGGAGCCGTCATGGAAGCGGTGGACGCCCGCGGGGCCCAGG
ACGAGGTGGTGGCGGCGGAGGGCAGGAGCAAGATGCAGGGGGTGATGTCC
TCGGAGCTGCCCACGCACCCGCAGATGCGCGAAGGCATGCTGCCCAACGG
GCTGCGTTACATCATCCTGAACAACCAGAGCCCTCCGGAGAGGTTCGAGG
CGCATCTGGAGGTG
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