mRNA_Ecto-sp9_F_contig8862.17045.1 (mRNA) Ectocarpus species9 EcSCA_722f female

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp9_F_contig8862.17045.1
Unique NamemRNA_Ecto-sp9_F_contig8862.17045.1
TypemRNA
OrganismEctocarpus species9 EcSCA_722f female (Ectocarpus species9 EcSCA_722f female)
Homology
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: Uncharacterized (protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LGT9_ECTSI)

HSP 1 Score: 238 bits (608), Expect = 4.980e-75
Identity = 140/165 (84.85%), Postives = 143/165 (86.67%), Query Frame = 1 
Query:    1 APVSPPTCPKAESAADENCDAGGSSDDSXXXXXSNLDLTSSDTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASA---SDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRLRASGD 486
            APVSP     AESAADENCDAG      XXXX SNLDLTSSDTVAYSSRMIAA+RALESRRRDRLFDDPYAELLAGPDAMQTVED    KAQE SS PPLEA AAA+A   SDGVPR+VQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRLRASGD
Sbjct:   37 APVSPXXXXXAESAADENCDAGXXXXXXXXXXSSNLDLTSSDTVAYSSRMIAAARALESRRRDRLFDDPYAELLAGPDAMQTVED----KAQEESSLPPLEATAAATAAAASDGVPRDVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRLRASGD 197
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: Uncharacterized (protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXP3_ECTSI)

HSP 1 Score: 146 bits (369), Expect = 4.040e-40
Identity = 79/92 (85.87%), Postives = 82/92 (89.13%), Query Frame = 1 
Query:  220 LAGPDAMQTVEDREKAKAQEGSSAPPLEA---AAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRLRASGD 486
            LAGPD +QTVEDREK KAQE  SAPPLEA   A AA+ASDGVPR+VQ RLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLR RASGD
Sbjct:   11 LAGPDPIQTVEDREKVKAQEELSAPPLEATTAATAAAASDGVPRDVQTRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRRRASGD 102
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: Hypothetical (protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UT70_HETAK)

HSP 1 Score: 81.3 bits (199), Expect = 4.340e-15
Identity = 48/118 (40.68%), Postives = 71/118 (60.17%), Query Frame = 1 
Query:  118 SSDTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            S+D VA+SS+ +AA+RA+E++RRDRLF+D  ++ LAG  A++  + + K +                    G PR   R ++IRT++ DDF E  +   GI+QIV +GAGMDTR  RL
Sbjct:   55 SADPVAFSSQRMAAARAIENKRRDRLFEDKVSQYLAGQ-ALEVAKQKMKTE-------------------KGYPRT--RNISIRTKYFDDFLEHVSVEHGIEQIVLVGAGMDTRAFRL 150
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: S-adenosyl-L-methionine-dependent (methyltransferase n=1 Tax=Oscillatoria sp. SIO1A7 TaxID=2607767 RepID=A0A7C9TUH9_9CYAN)

HSP 1 Score: 77.0 bits (188), Expect = 5.710e-14
Identity = 52/116 (44.83%), Postives = 63/116 (54.31%), Query Frame = 1 
Query:  124 DTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            D V+ SS+ +AA RALE++R DRLFDDPYAE LAG + +  V+             P L+         G     +   A+RTRF DDF    A P  I QIV LGAGMDTR  RL
Sbjct:    6 DIVSRSSQFMAAVRALETKRPDRLFDDPYAERLAGAEIIAQVK-------------PILK---------GADERGRPFAAVRTRFFDDFLFSLASP--INQIVILGAGMDTRAFRL 97
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: S-adenosyl-L-methionine-dependent (methyltransferase n=1 Tax=Thermoleophilaceae bacterium TaxID=2732252 RepID=A0A7V9S157_9ACTN)

HSP 1 Score: 76.3 bits (186), Expect = 1.560e-13
Identity = 47/116 (40.52%), Postives = 59/116 (50.86%), Query Frame = 1 
Query:  124 DTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            D +A +SR +AA+RA ES R DRLFDDP A  LAGP+    +E  E A   +G    P+                     IRTRF DDF  D     G++Q+V   AG+DTR  RL
Sbjct:    6 DALAPTSRWMAAARARESERADRLFDDPLAAALAGPEGFAWLEQMEAAAGSDGPGLYPV---------------------IRTRFFDDFLLDSCRRLGVRQVVLAAAGLDTRAFRL 100
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: SAM-dependent (methyltransferase n=2 Tax=Streptomyces sp. TaxID=1931 RepID=UPI00184D101A)

HSP 1 Score: 73.9 bits (180), Expect = 1.080e-12
Identity = 49/114 (42.98%), Postives = 63/114 (55.26%), Query Frame = 1 
Query:  130 VAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            VA ++R  AA+RA ESRR DRLFDDP +ELLAG D  Q ++    A+A +                +G P      L IRTR+ DDF        G++QIV +GAG+DTR  RL
Sbjct:   27 VAQTARWTAAARARESRRPDRLFDDPLSELLAGEDGEQLLKYFHTARASD----------------EGNPF-----LPIRTRWFDDFLARSTAD-GVRQIVGIGAGLDTRSFRL 118
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: S-adenosyl-L-methionine-dependent (methyltransferase n=1 Tax=Sinosporangium album TaxID=504805 RepID=A0A1G8A5R7_9ACTN)

HSP 1 Score: 73.6 bits (179), Expect = 1.340e-12
Identity = 52/114 (45.61%), Postives = 61/114 (53.51%), Query Frame = 1 
Query:  130 VAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            VA ++R  AASRA ESRR DRLFDDP A LLAGP A   +     ++A                A +G P      L IRTR+ DDF    AG  G  Q+V LGAG+DTR  RL
Sbjct:   15 VAQTARWTAASRARESRRPDRLFDDPLAALLAGPHAAALLRHFHTSRA----------------ADEGNPF-----LPIRTRWFDDFLRAKAGKTG-HQVVGLGAGLDTRAHRL 106
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: SAM-dependent (methyltransferase n=2 Tax=Pseudanabaena sp. FACHB-2040 TaxID=2692859 RepID=UPI001689B4AA)

HSP 1 Score: 73.6 bits (179), Expect = 1.440e-12
Identity = 49/122 (40.16%), Postives = 67/122 (54.92%), Query Frame = 1 
Query:  106 LDLTSSDTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQT-VEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLR 468
            +   +SD ++Y++R++AA RA+E     RLF+DPYA LLAG +     V+ RE A+ Q+     PLE        D + +   R +AIRTRF DD            QIV LGAG+DTR  R
Sbjct:    1 MSSAASDPISYTARIVAAKRAIEQSHPGRLFEDPYAALLAGDEVENLLVKWREVARRQDR----PLE--------DVIVKRT-RYIAIRTRFFDDLLRSALSETDNSQIVILGAGLDTRAFR 109
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: S-adenosyl-L-methionine-dependent (methyltransferase n=1 Tax=uncultured Rubrobacteraceae bacterium TaxID=349277 RepID=A0A6J4QRY9_9ACTN)

HSP 1 Score: 72.8 bits (177), Expect = 2.460e-12
Identity = 48/117 (41.03%), Postives = 58/117 (49.57%), Query Frame = 1 
Query:  121 SDTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            S+ VA +SR +AA+RA ES R DRLFDDP A  LAGP+    +E  E A    G    P+                     IRTRF DDF        GI+Q+V   AG+DTR  RL
Sbjct:    7 SNPVAPTSRWMAAARARESERPDRLFDDPLAAALAGPEGFAWLERMEAAAQSSGPGLYPV---------------------IRTRFFDDFLLKACKESGIRQVVLAAAGLDTRAFRL 102
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BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Match: S-adenosyl-L-methionine-dependent (methyltransferase n=1 Tax=Cyanobacteria bacterium UBA11367 TaxID=2055774 RepID=A0A352XFW5_9CYAN)

HSP 1 Score: 72.8 bits (177), Expect = 2.460e-12
Identity = 46/123 (37.40%), Postives = 63/123 (51.22%), Query Frame = 1 
Query:  103 NLDLTSSDTVAYSSRMIAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPLEAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGMDTRGLRL 471
            N   T  + V+ S++++AA RA E+ R DRLF DP+A  LAG  A++  ++RE                      +    +V  RLAIRTRF DDF  D      ++Q+V L AGMD R  RL
Sbjct:   12 NQSFTIENFVSLSAQLVAAVRARETARNDRLFTDPFAAELAGKQALEMAKERE----------------------ERTKDSVADRLAIRTRFFDDFLIDAVSE--VRQVVILAAGMDARAFRL 110
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp9_F_contig8862.17045.1 vs.
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
Uncharacterized4.980e-7584.85protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LGT9... [more]
Uncharacterized4.040e-4085.87protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 ... [more]
Hypothetical4.340e-1540.68protein n=2 Tax=Heterosigma akashiwo TaxID=2829 Re... [more]
S-adenosyl-L-methionine-dependent5.710e-1444.83methyltransferase n=1 Tax=Oscillatoria sp. SIO1A7 ... [more]
S-adenosyl-L-methionine-dependent1.560e-1340.52methyltransferase n=1 Tax=Thermoleophilaceae bacte... [more]
SAM-dependent1.080e-1242.98methyltransferase n=2 Tax=Streptomyces sp. TaxID=1... [more]
S-adenosyl-L-methionine-dependent1.340e-1245.61methyltransferase n=1 Tax=Sinosporangium album Tax... [more]
SAM-dependent1.440e-1240.16methyltransferase n=2 Tax=Pseudanabaena sp. FACHB-... [more]
S-adenosyl-L-methionine-dependent2.460e-1241.03methyltransferase n=1 Tax=uncultured Rubrobacterac... [more]
S-adenosyl-L-methionine-dependent2.460e-1237.40methyltransferase n=1 Tax=Cyanobacteria bacterium ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp9_F_contig8862contigEcto-sp9_F_contig8862:190..2779 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Ectocarpus species9 EcSCA_722f female vs UniRef902022-09-19
OGS1.0 of Ectocarpus species9 EcSCA_722f female2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score240.7
Seed ortholog evalue4.6e-61
Seed eggNOG ortholog2880.D8LGT9
EggNOG free text desc.Leucine carboxyl methyltransferase
EggNOG OGs2QRNU@2759,COG3315@1
COG Functional cat.Q
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Hectar predicted targeting categoryother localisation
Exons3
Model size488
Cds size486
Stop0
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp9_F_contig8862.17045.1prot_Ecto-sp9_F_contig8862.17045.1Ectocarpus species9 EcSCA_722f femalepolypeptideEcto-sp9_F_contig8862 190..2779 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622930939.5360463-CDS-Ecto-sp9_F_contig8862:189..2491622930939.5360463-CDS-Ecto-sp9_F_contig8862:189..249Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 190..249 -
1692278958.1662354-CDS-Ecto-sp9_F_contig8862:189..2491692278958.1662354-CDS-Ecto-sp9_F_contig8862:189..249Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 190..249 -
1622930939.552113-CDS-Ecto-sp9_F_contig8862:1325..15291622930939.552113-CDS-Ecto-sp9_F_contig8862:1325..1529Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 1326..1529 -
1692278958.181437-CDS-Ecto-sp9_F_contig8862:1325..15291692278958.181437-CDS-Ecto-sp9_F_contig8862:1325..1529Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 1326..1529 -
1622930939.5643623-CDS-Ecto-sp9_F_contig8862:2557..27791622930939.5643623-CDS-Ecto-sp9_F_contig8862:2557..2779Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 2558..2779 -
1692278958.190165-CDS-Ecto-sp9_F_contig8862:2557..27791692278958.190165-CDS-Ecto-sp9_F_contig8862:2557..2779Ectocarpus species9 EcSCA_722f femaleCDSEcto-sp9_F_contig8862 2558..2779 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp9_F_contig8862.17045.1

>prot_Ecto-sp9_F_contig8862.17045.1 ID=prot_Ecto-sp9_F_contig8862.17045.1|Name=mRNA_Ecto-sp9_F_contig8862.17045.1|organism=Ectocarpus species9 EcSCA_722f female|type=polypeptide|length=162bp
APVSPPTCPKAESAADENCDAGGSSDDSNDSSSSNLDLTSSDTVAYSSRM
IAASRALESRRRDRLFDDPYAELLAGPDAMQTVEDREKAKAQEGSSAPPL
EAAAAASASDGVPRNVQRRLAIRTRFCDDFFEDCAGPRGIKQIVSLGAGM
DTRGLRLRASGD
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mRNA from alignment at Ecto-sp9_F_contig8862:190..2779-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp9_F_contig8862.17045.1 ID=mRNA_Ecto-sp9_F_contig8862.17045.1|Name=mRNA_Ecto-sp9_F_contig8862.17045.1|organism=Ectocarpus species9 EcSCA_722f female|type=mRNA|length=2590bp|location=Sequence derived from alignment at Ecto-sp9_F_contig8862:190..2779- (Ectocarpus species9 EcSCA_722f female)
GCGCCCGTGAGCCCACCAACATGTCCCAAAGCAGAGTCGGCAGCAGACGA
GAATTGTGATGCAGGAGGCAGCAGCGATGACTCCAACGACAGCAGCAGCA
GCAATCTGGACTTGACCTCCTCTGACACCGTGGCCTACTCCTCGCGGATG
ATCGCGGCGTCCAGAGCGTTGGAGTCGCGACGCCGGGACCGCTTGTTCGA
CGACCCTTACGCCGAGCTGCTGGTGAGGGGACCGCCGTATCCCGAGGATA
ATGATGAGCGTTACGAAGTATGTGAGCTAGAGAGAGGTGTTTTCGTAGCC
CTAGCCCCGGACCCAACACCATATCCCCTTCTAAAACCAAGCTTAATAAC
GTCCTCGCGACACGCTACTGATTTGAGGGGCGTTCGTTGGAGCGAAGCAT
ACACAGTCGTACGTGCTTGCAACACAAGAGAACACACCCCGTCGACAATC
TGTATCTAACATTTTGGATTTTATTCATCAACTTGTAGGGTGTGTTATCG
CTAGGGGCGCACGGTACATTACTTGTGTCGCCTAGGCACTTGATTTTGAT
GTGCGCGTAGTTTTGTACTCCGGACTCGTGTTGACCCTTGACAGTATTGC
CACCCGGTGGGGAGGGTGGTGGTATCTTGTGTGAGTTGGTGTGAACTTGT
TGCGAATACGGAAGTCTGTCTGCTCCGCATGGTCGCTTTGGAAAAATCAC
CGTCGCTCAGTGAGTCTCGCGCAGTGCGGAACATGGGCCTACCGCATCCA
CTCCGCCGTAGGCATCGGATGCGATTTGCTTCTTGACAGCAGTGTCATGC
TTGCCCTCAGTGGGCCGAAAAGTTTCGATCGGGACCGCCACCACTTGCCA
TGCCTCTGGTAACGGTGGCGCTCGATATTGCTTTTGTGCGTCCGGGTCTT
TCTTCCTTGGGCCATCTAATCCCCGCCTCCCCACCCCCGAATGCCTACAG
TTGGGCAAAACCAAAAACATCGAGCCACCTTTGGCGAAGTAGGAAGGAAT
GACTGGCGATCGGTGATCGGTCCTCTGTTGGAACAATGCTCCGTCCTCTC
GAGGTCAATGGTATACCAACGGCATCGCCCCCCCCCCCCAGTGCCTCCAG
CTTCTTGGTCATTCTTGTGAAACGTAGCCTGGGCCGGCGAAACTCGGCAT
TTTGCTCCGTCCCGGCATTGTAAGGAATCGAGGTCAGTGGACCAACGACT
ACGGCATGCGTTTACGTGAGTATCCCCCCTCCGTCTACTTGTTGCTTCAG
GCCGGCCCCGATGCCATGCAGACCGTCGAGGACCGGGAGAAGGCCAAAGC
GCAAGAGGGGTCGTCAGCACCACCTCTGGAAGCCGCCGCCGCCGCCTCCG
CAAGCGACGGTGTCCCGAGAAACGTCCAGAGGCGGCTGGCCATCCGCACA
CGCTTCTGCGACGACTTCTTCGAGGACTGCGCAGGGCCGAGGGGTATCAA
GCAGGTTAGCCTAGCACATAATAGTCGGGACCAAAACAAAAATACCGGGC
CTAATTTTCGCTCTTGCAGTATGATCTTCACTCTTGTAGTATGATCTTCA
GTAGATTATATGATAGCGCGAAGGGACGGCACGGCTTTCCAACCTCGAAC
GCAAAAATCCCACTGAAAAACAACGTCGGTGGTACAAAGTTGCACCCCAC
ACACGCTGTTTCATTTGCTCCAATTTGGTCCTGACTATTGTGTGTTTCCG
AAAGGGTGGCCCCTGTATCTAGGCAGGTTCGCCTAGTTGTGGTCTTAGTT
AGAATGCCGTATGGTTCCAAGAGGGGCATATGAAGCAGGTTAGCCTAACG
TAGGGGTCCCTGTTTAAGGCCACATGGTGCCAAGGGGTTTTGGCCAGCCT
TGCGCCGGACACCCGGCGCTATTCAGAGTTTGAGACAGCTTGAATATTCA
ATGTGCTTCACACGCGATTTCACCGGCGACGGGCGTTGTGCAACGACGTC
CCCTACTGCACCTGCCTGGGGAGTGGTGGTGGATAACCAGAAACGGGCAT
CGCCCGAATTACGGGCACGTACTGGAATCGCTTTGCTCGTAGCCATATCG
ATTAATTCCTGCTTCGCTCCCTGACGCCACCCGAATCTATCTCTTCGCCC
GGACTGTCGCAGTGCGCAACTTTGCGCTTAGTTTTTTCGTAACGTAGCGT
ACGTCCCCGAGGCCCTACCCCACAGCAGCCAAGCAGCACGCGGGTTGTCC
ACTTGCGTCCTGCAAGGAAAAACGGAGCATTCGGTATGAAGTTTGATCGG
CGCAAGTGCGCGCGCGAGGGGGGGGCGGGGGGGGCGTGCAGGTGGTTCCA
TGCTGCGTGAGAGAACCTTTAAGCCTGTTGCTCGGACGCCCCAAACCCGG
TACTCGGCTTGTCGATCGTGGCCGGTCGTCTCCAAAGCCCAACACAATCC
ACGCTCGTGTCTTCTCACAGTCAACCCCTCTCCTCCACACAAATATGGCC
TCACAGACCTCTTGTGTGCTACGGCGTTCCCCGGACGCTGACCCTGCTAT
CTCTACCCGTGGCCCTTTGTACGTCTGCAGATCGTGTCGTTGGGGGCGGG
CATGGACACGAGGGGGCTACGGCTCAGGGCTTCGGGGGAC
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Coding sequence (CDS) from alignment at Ecto-sp9_F_contig8862:190..2779-

>mRNA_Ecto-sp9_F_contig8862.17045.1 ID=mRNA_Ecto-sp9_F_contig8862.17045.1|Name=mRNA_Ecto-sp9_F_contig8862.17045.1|organism=Ectocarpus species9 EcSCA_722f female|type=CDS|length=972bp|location=Sequence derived from alignment at Ecto-sp9_F_contig8862:190..2779- (Ectocarpus species9 EcSCA_722f female)
GCGCCCGTGAGCCCACCAACATGTCCCAAAGCAGAGTCGGCAGCAGACGA
GAATTGTGATGCAGGAGGCAGCAGCGATGACTCCAACGACAGCAGCAGCA
GCAATCTGGACTTGACCTCCTCTGACACCGTGGCCTACTCCTCGCGGATG
ATCGCGGCGTCCAGAGCGTTGGAGTCGCGACGCCGGGACCGCTTGTTCGA
CGACCCTTACGCCGAGCTGCTGGCGCCCGTGAGCCCACCAACATGTCCCA
AAGCAGAGTCGGCAGCAGACGAGAATTGTGATGCAGGAGGCAGCAGCGAT
GACTCCAACGACAGCAGCAGCAGCAATCTGGACTTGACCTCCTCTGACAC
CGTGGCCTACTCCTCGCGGATGATCGCGGCGTCCAGAGCGTTGGAGTCGC
GACGCCGGGACCGCTTGTTCGACGACCCTTACGCCGAGCTGCTGGCCGGC
CCCGATGCCATGCAGACCGTCGAGGACCGGGAGAAGGCCAAAGCGCAAGA
GGGGTCGTCAGCACCACCTCTGGAAGCCGCCGCCGCCGCCTCCGCAAGCG
ACGGTGTCCCGAGAAACGTCCAGAGGCGGCTGGCCATCCGCACACGCTTC
TGCGACGACTTCTTCGAGGACTGCGCAGGGCCGAGGGGTATCAAGCAGGC
CGGCCCCGATGCCATGCAGACCGTCGAGGACCGGGAGAAGGCCAAAGCGC
AAGAGGGGTCGTCAGCACCACCTCTGGAAGCCGCCGCCGCCGCCTCCGCA
AGCGACGGTGTCCCGAGAAACGTCCAGAGGCGGCTGGCCATCCGCACACG
CTTCTGCGACGACTTCTTCGAGGACTGCGCAGGGCCGAGGGGTATCAAGC
AGATCGTGTCGTTGGGGGCGGGCATGGACACGAGGGGGCTACGGCTCAGG
GCTTCGGGGGACATCGTGTCGTTGGGGGCGGGCATGGACACGAGGGGGCT
ACGGCTCAGGGCTTCGGGGGAC
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