mRNA_Ecto-sp8_S_contig10382.623.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_Ecto-sp8_S_contig10382.623.1
Unique Name	mRNA_Ecto-sp8_S_contig10382.623.1
Type	mRNA
Organism	Ectocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)

Homology

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5JRJ6_9PHAE (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JRJ6_9PHAE)

HSP 1 Score: 159 bits (401), Expect = 5.460e-41
Identity = 90/164 (54.88%), Postives = 104/164 (63.41%), Query Frame = 3

Query:    6 YAVGKHKDHLPDCRSERRWPS-----RLLQQS*GLSPRTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGG-AWSFYDDEALCVRVDESSDVSGRTPLSHLAV 479
            +A+GK+K  L D R  R         R L        RTLLA + ASP LA S+LLEFN RWNLRMM+KNRGLPAA GGHYN+YL + IQR TADW+SS  V P WPAAID V TGER    RS W GE  G  W   D++A C   D  SDV+GRTP+S L +
Sbjct:  638 FAIGKNKHGLTDYRCVRGTNDVEGYHRYL--------RTLLASYCASPSLAQSVLLEFNYRWNLRMMIKNRGLPAALGGHYNQYLVESIQRDTADWDSSHPVFPEWPAAIDSVDTGERFGLERSLWGGERDGEVWGIADNDAFCTD-DGDSDVNGRTPMSDLTL 792

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5K4M0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K4M0_9PHAE)

HSP 1 Score: 113 bits (282), Expect = 4.680e-25
Identity = 71/158 (44.94%), Postives = 81/158 (51.27%), Query Frame = 3

Query:    6 YAVGKHKDHLPDCRSERRWPSRLLQQS*GLSPRTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGGAWSFYDDEALCVRVDESSDVSGRTPLSHLAV 479
            YA+GK  D L   R  R           G      LA +SASP LA S+LLEFN RWNL+MMVKNR                       DW+ S  V P W AA+D V TGERS   RS W GE   AW+    EA C  VD+  DV+GRTPLSHLA+
Sbjct:  930 YAIGKTTDGLVRYRCVRG-----TNDVEGYH----LASYSASPALAQSVLLEFNFRWNLKMMVKNR-----------------------DWDPSFPVFPQWQAAVDSVDTGERSGLGRSLWGGENAKAWNGAGHEASCAEVDDVGDVNGRTPLSHLAL 1055

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: D7FWS6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FWS6_ECTSI)

HSP 1 Score: 92.8 bits (229), Expect = 3.300e-21
Identity = 42/59 (71.19%), Postives = 50/59 (84.75%), Query Frame = 3

Query:  186 MMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRS 362
            M+VKNRGLPAA GGHYN+YL +L+QR TADWE+SC VPP +PAA+D  GTGERS  +RS
Sbjct:    1 MLVKNRGLPAALGGHYNEYLVELVQRATADWEASCPVPPQYPAAMDPEGTGERSGLARS 59

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5K9U3_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K9U3_9PHAE)

HSP 1 Score: 99.0 bits (245), Expect = 1.420e-20
Identity = 55/124 (44.35%), Postives = 71/124 (57.26%), Query Frame = 3

Query:  102 RTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGGAWSFYDDEALCVRVDESSDVSGRTPLSHL 473
            R LL+ + ASP LAHS+LLEFN RWN+RM VKNRGLP   G  +++Y  ++IQR TA W     + P W +A+D   TGERS  S+S W    GG       +A    VDE +      P+  L
Sbjct:   74 RRLLSSYCASPALAHSVLLEFNFRWNVRMAVKNRGLPREVGEFFDQYETEIIQRDTASWYPDNPLFPEWVSALDVADTGERSGLSQSMWGPASGG-------DAPGDLVDEQASPQHAIPMPKL 190

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5JPK9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPK9_9PHAE)

HSP 1 Score: 94.4 bits (233), Expect = 1.110e-18
Identity = 47/95 (49.47%), Postives = 61/95 (64.21%), Query Frame = 3

Query:  102 RTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGG 386
            R LL+ + ASP LAHS+LLEFN RWN+RM VKNRGLP   G  ++++  ++IQR TA W     + P W +A+D   TGE+S  S S W    GG
Sbjct:  347 RRLLSTYCASPSLAHSVLLEFNYRWNVRMAVKNRGLPREVGEFFDQFEIEIIQRETASWYPDSPLFPEWVSALDAADTGEQSGLSESLWGPASGG 441

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5KLP1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLP1_9PHAE)

HSP 1 Score: 94.4 bits (233), Expect = 1.350e-18
Identity = 47/95 (49.47%), Postives = 61/95 (64.21%), Query Frame = 3

Query:  102 RTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGG 386
            R LL+ + ASP LAHS+LLEFN RWN+RM VKNRGLP   G  ++++  ++IQR TA W     + P W +A+D   TGE+S  S S W    GG
Sbjct:  344 RRLLSTYCASPSLAHSVLLEFNYRWNVRMAVKNRGLPREVGEFFDQFEIEIIQRETASWYPDSPLFPEWVSALDAADTGEQSGLSESLWGPASGG 438

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5JEJ9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JEJ9_9PHAE)

HSP 1 Score: 82.4 bits (202), Expect = 3.380e-17
Identity = 41/52 (78.85%), Postives = 44/52 (84.62%), Query Frame = 2

Query:  239 VSWEADPADHGGLGVKLLRSPGV-AGRHRLSRHGREELALQELMGGRGRGGM 391
            +SWE DP  H GLG++L R PGV AGRHRLSRHG EELALQELMGGRGRGGM
Sbjct:   15 LSWETDPTGHDGLGIELPRPPGVVAGRHRLSRHGLEELALQELMGGRGRGGM 66

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: D7G3Q9_ECTSI (3'-5' exonuclease domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3Q9_ECTSI)

HSP 1 Score: 86.7 bits (213), Expect = 6.200e-16
Identity = 50/93 (53.76%), Postives = 58/93 (62.37%), Query Frame = 3

Query:    6 YAVGKHKDHLPDCRSERRWPS-----RLLQQS*GLSPRTLLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTT 269
            YAVGK  D L + R  R         R L        RTLLA + ASP LA S+LLEFN RWNL+MMVKNRGLPA  G HYN++L ++IQR T
Sbjct:  682 YAVGKTTDGLVNYRCVRGTNDVEGYHRYL--------RTLLASYCASPALAQSVLLEFNFRWNLKMMVKNRGLPAELGEHYNQHLVEIIQRAT 766

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5L7E8_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L7E8_9PHAE)

HSP 1 Score: 74.3 bits (181), Expect = 3.710e-14
Identity = 34/38 (89.47%), Postives = 34/38 (89.47%), Query Frame = 3

Query:  150 MLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQR 263
            MLLEFNL WNLRMMVKNRGLPAA GGHYN Y GKLIQR
Sbjct:    1 MLLEFNLGWNLRMMVKNRGLPAALGGHYNNYRGKLIQR 38

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BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Match: A0A6H5KP32_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KP32_9PHAE)

HSP 1 Score: 78.6 bits (192), Expect = 3.890e-14
Identity = 47/125 (37.60%), Postives = 65/125 (52.00%), Query Frame = 3

Query:  108 LLACFSASPPLAHSMLLEFNLRWNLRMMVKNRGLPAAPGGHYNKYLGKLIQRTTADWESSCSVPPAWPAAID*VGTGERSWRSRSSWEGEVGGAWSFYDDEALCVRVDESSDVSGRTPLSHLAVP 482
            LL+ + ASP LAHS+LLEFN RWN++M VKNR LP   GG ++++  ++IQR T  W+    V     +A+D   TG+RS  ++S   G   G              D   D  G   +   AVP
Sbjct:   84 LLSTYCASPALAHSILLEFNHRWNIKMAVKNRNLPKEIGGCFDQFDIEIIQRETTSWDLDSPVFSERVSALDAADTGQRSGLAQSMCGGADAG--------------DSGVDALGEQEMVQNAVP 194

The following BLAST results are available for this feature:

BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot

BLAST of mRNA_Ecto-sp8_S_contig10382.623.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 18


Match Name	E-value	Identity	Description
A0A6H5JRJ6_9PHAE	5.460e-41	54.88	3'-5' exonuclease domain-containing protein n=1 Ta...	[more]
A0A6H5K4M0_9PHAE	4.680e-25	44.94	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
D7FWS6_ECTSI	3.300e-21	71.19	Uncharacterized protein n=1 Tax=Ectocarpus silicul...	[more]
A0A6H5K9U3_9PHAE	1.420e-20	44.35	Uncharacterized protein n=2 Tax=Ectocarpus sp. CCA...	[more]
A0A6H5JPK9_9PHAE	1.110e-18	49.47	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
A0A6H5KLP1_9PHAE	1.350e-18	49.47	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
A0A6H5JEJ9_9PHAE	3.380e-17	78.85	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
D7G3Q9_ECTSI	6.200e-16	53.76	3'-5' exonuclease domain-containing protein n=1 Ta...	[more]
A0A6H5L7E8_9PHAE	3.710e-14	89.47	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
A0A6H5KP32_9PHAE	3.890e-14	37.60	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Ecto-sp8_S_contig10382	contig	Ecto-sp8_S_contig10382:3467..6265 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
Ectocarpus species8 EcLAC_412 OGS1.0	2022-07-08

Properties


Property Name	Value
Stop	1
Start	1
Model size	686
Hectar predicted targeting category	other localisation
Exons	2
Cds size	348

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681143137.5298257-UTR-Ecto-sp8_S_contig10382:3466..3524	1681143137.5298257-UTR-Ecto-sp8_S_contig10382:3466..3524	Ectocarpus species8 EcLAC_412	UTR	Ecto-sp8_S_contig10382 3467..3524 +
1681143137.5597124-UTR-Ecto-sp8_S_contig10382:5985..6265	1681143137.5597124-UTR-Ecto-sp8_S_contig10382:5985..6265	Ectocarpus species8 EcLAC_412	UTR	Ecto-sp8_S_contig10382 5986..6265 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681143137.5402348-CDS-Ecto-sp8_S_contig10382:3524..3556	1681143137.5402348-CDS-Ecto-sp8_S_contig10382:3524..3556	Ectocarpus species8 EcLAC_412	CDS	Ecto-sp8_S_contig10382 3525..3556 +
1681143137.5496888-CDS-Ecto-sp8_S_contig10382:5669..5985	1681143137.5496888-CDS-Ecto-sp8_S_contig10382:5669..5985	Ectocarpus species8 EcLAC_412	CDS	Ecto-sp8_S_contig10382 5670..5985 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_Ecto-sp8_S_contig10382.623.1	prot_Ecto-sp8_S_contig10382.623.1	Ectocarpus species8 EcLAC_412	polypeptide	Ecto-sp8_S_contig10382 3525..5985 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at Ecto-sp8_S_contig10382:3467..6265+
coding sequence from alignment at Ecto-sp8_S_contig10382:3467..6265+

protein sequence of mRNA_Ecto-sp8_S_contig10382.623.1

>prot_Ecto-sp8_S_contig10382.623.1 ID=prot_Ecto-sp8_S_contig10382.623.1|Name=mRNA_Ecto-sp8_S_contig10382.623.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=116bp
MAFATAAAELRAITADPAGLLQCISTTSALDVAGVQPPVEPEDDGQEPWL
ARGAGRTLQQVSWEADPADHGGLGVKLLRSPGVAGRHRLSRHGREELALQ
ELMGGRGRGGMEFLR*

mRNA from alignment at Ecto-sp8_S_contig10382:3467..6265+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_Ecto-sp8_S_contig10382.623.1 ID=mRNA_Ecto-sp8_S_contig10382.623.1|Name=mRNA_Ecto-sp8_S_contig10382.623.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=2799bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10382:3467..6265+ (Ectocarpus species8 EcLAC_412)
TGTAGTACGCCGTTGGCAAGCACAAGGATCACCTTCCCGACTGCAGGAGC
GAACGAAGATGGCCTTCGCGACTGCTGCAGCAGAGTTAAGGTGAGATTGT
GCTTCCTGATCGGAAATAAACGAGCTGGTTTGCGTGCACGAAGGCGGGGA
TACCCCTGTACGTCTGTCTCTGCTGTGTGTTGCCTTGAGGCTACATACAG
CAGCAGTCTAGAGGCTACAGCAGCAGTCTAGGTGTTTAGCGAGAAGGACG
GCGGTGCGTAGTTTAGTTGGTGATCTCGCCAGGACGTTTGTTTGCTCTTC
GTCTCTTCTGGGTCAGTCGACTGGTTTATGTGTCCGCGCACCTGAGACCG
AACAACAACTGAACATCATCAATCATCAGACAACATCATCACACGGTTTG
CTGGTTCAGGCAGGATCCAAACACATTCGACGCATGATCTGTACCGCAAA
CACACATGGCCTGGAGGGGTTGAAGGCAAAGGGCGGTACTGGTATAGACT
ATCGACTCGAAAGCAGTAGCCGTATTTTGTGTCCATGTTGTCTACATAAT
TTACAAGGCATCTACAGCATCAGTAGTAGTGTAGACCACGGCGGTACAAC
AGCAGTATACAGAGTAACGGTGTGCGGTAGCCCAGTCCACCCTCCGTCGC
CACCGTGACGTTAACCGCTCCTGTCGTCGCGGCTCCCATCAACGGTGCCG
CCGCTCCGTCCGTGAGTTCACACCCGGCCGGCCGCTGCCGCGGGGGAAAC
ATGGCCGCTGGCGGTTCCCCCTCTTGCCGCCGGTGCTGCTGCGGATGCTG
CTGCTCTATCTAGCCGCGAGTGCCTGCGCCCCGGCCGTCTGCCCCTCCGC
CGCCGCGAGGAAAACTCTGCCGCCGGGGGTCGCCATTCTGTCAACGCTCA
TACACTAGTTGCTCCACTACAATTCTCGCCGCCGGTTCTGCTACTGATGG
TACCGCTATATCCTCGAGTGCCTGCACCCCGGCCGCCCCCACGGCCGGCA
CCGCGGGGAAAACTCTGCTGCCGGGGCTCACCCCTCTCGCCGACGGTGCT
GCCACCGATGGTACCGCTCTAGCCGCGAGTGTCTGCACCCCGGCCGCCCC
CCCGGCCGGCGTCGCGGGGAAAACATGACTGCCGGGGGTCACCCCTTCGC
CGGTGATGCTGCTGCCGATGGTGCCGCTCCCTCCGCGAGTGCCTGCGCCC
GGCCGCCTTTCCCGCCGCCGCCGCCGCCGCCGGGGGGCACCCCCCTCACC
GCCGGTGCCGCTGCGACCCAAACGACCAACATACTTAAGGCCCTGTCAGA
CCATCGGGGAGAACTGCTTGGAGGAGTATTTTCATGCTATTTCTTTCGTA
TATCTTCTTCTATTGTGTATCCAGCGCAGTAGTAACTACAATAGCGGGCA
CATCCTATCTGCAACATGAACTGCTGTAGATACCTTTTTCGGCGTCGGAG
TCCCAACCTGAGATGCTACAATATCAATTAAGGACCATGCGTTAACTCTG
TAGCGACGTATGTGCTGGCGCGCCCACAGGTCTGTCACTGGGGTATGTAA
GGGTATCGAAGAACTTACTGTAGCATACATACCTGCTGTACCAAATCGTC
CGTAAACGAAAACGTTTGACACTGACTGCTGTACTGTAGTCGCGACCACA
TGCCCAAGGAACAGCCATGCATTCTGTGGCATGAGCTGCTGCTGCTGCTG
CTGCTGCTGCTGTAGTAGACTAGAGCAGTAGACCCGCACTTCAGGAGGCT
GTGTTTGTGACGACGAGCGCTGTTTCGAAAGATGATTCCGCTTCGCAGGC
TTTTGGAACGCAGTTGAAGCCTTCAACAGTACAGCCCTAAGATCAGCACA
TCTGAGCATGCCTGCATTGCCGTGTCGAAACTCCATGATGTCCGTCACTG
ACGTAGCATTTCAGGTTGGGAACCCGATAGAACGAAGCCCCTGTAACCCT
TGTCCGACAAGTCGCCTGCGGCGTCTGGCTGGTATCTGGGACCATGATTT
TTTTTAGCTTCTGTGATAGCTTTTGTGTTGATGTTTCTCGGCCTTTCATT
TGCTCTTCAGGTTTTCAAATTGCATCCTTGGTAGGGCCTGTGCCCACGGA
AGGCATCTCCGTCAGTTGCACCAGTAAGGATGTTAGAAGGTTGTAAATTC
ACGGCTTCCACCCCTCTTGTCGCGTCCCAACCGCTGTTCACTTCTTACAA
CAGGGCTATCACCGCGGACCCTGCTGGCCTGCTTCAGTGCATCTCCACCA
CTAGCGCACTCGATGTTGCTGGAGTTCAACCTCCGGTGGAACCTGAGGAT
GATGGTCAAGAACCGTGGCTTGCCCGCGGCGCCGGGAGGACACTACAACA
AGTATCTTGGGAAGCTGATCCAGCGGACCACGGCGGACTGGGAGTCAAGT
TGCTCCGTTCCCCCGGCGTGGCCGGCCGCCATCGACTAAGTAGGCACGGG
CGAGAGGAGTTGGCGCTCCAGGAGCTCATGGGAGGGAGAGGTCGGGGGGG
CATGGAGTTTCTACGATGATGAGGCGTTGTGTGTTCGTGTGGACGAAAGC
AGCGATGTTAGCGGCCGAACGCCGTTGTCGCACCTAGCGGTTCCTCCAAG
CCCATGCTTTCCTCGGAGCGATTCACGGCAAGGATGAGCGAAAAAATGCC
AGAATCGAGGGTCCACACCAGGGAGGAGAAGAAACGCAACTTCAGAGACG
ACCTGATGCTGCTGGTGGCAGGGGAACAACATGGGAGGACTCCAGCGGGA
ACAACCGCTCTCCGTTTCGACCCTTAACAAAACGGGGGCGGGGCTGCGT

Coding sequence (CDS) from alignment at Ecto-sp8_S_contig10382:3467..6265+

>mRNA_Ecto-sp8_S_contig10382.623.1 ID=mRNA_Ecto-sp8_S_contig10382.623.1|Name=mRNA_Ecto-sp8_S_contig10382.623.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=348bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10382:3467..6265+ (Ectocarpus species8 EcLAC_412)
ATGGCCTTCGCGACTGCTGCAGCAGAGTTAAGGGCTATCACCGCGGACCC
TGCTGGCCTGCTTCAGTGCATCTCCACCACTAGCGCACTCGATGTTGCTG
GAGTTCAACCTCCGGTGGAACCTGAGGATGATGGTCAAGAACCGTGGCTT
GCCCGCGGCGCCGGGAGGACACTACAACAAGTATCTTGGGAAGCTGATCC
AGCGGACCACGGCGGACTGGGAGTCAAGTTGCTCCGTTCCCCCGGCGTGG
CCGGCCGCCATCGACTAAGTAGGCACGGGCGAGAGGAGTTGGCGCTCCAG
GAGCTCATGGGAGGGAGAGGTCGGGGGGGCATGGAGTTTCTACGATGA