mRNA_Ecto-sp8_S_contig103217.526.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig103217.526.1
Unique NamemRNA_Ecto-sp8_S_contig103217.526.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A455U9V9_9GAMM (MscS_porin domain-containing protein n=1 Tax=Halomonas sulfidaeris TaxID=115553 RepID=A0A455U9V9_9GAMM)

HSP 1 Score: 263 bits (672), Expect = 3.070e-85
Identity = 154/175 (88.00%), Postives = 166/175 (94.86%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE+LEG LTAQQTSDKEALEA+LDAYERL ++ ERL+AL+ RVEQAPEL++RLQRELNEAE+ASQQLSV +LSD PLDELETLQT AVVELQ+LQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALND RLI+ RLERALADREVSFYQRELS
Sbjct:   37 LETLEGSLTAQQTSDKEALEASLDAYERLTSLEERLAALDTRVEQAPELLARLQRELNEAEDASQQLSVADLSDLPLDELETLQTGAVVELQELQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDPRLIQLRLERALADREVSFYQRELS 211
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A7Z0S010_9GAMM (Mechanosensitive channel MscK n=34 Tax=Halomonadaceae TaxID=28256 RepID=A0A7Z0S010_9GAMM)

HSP 1 Score: 268 bits (684), Expect = 1.570e-80
Identity = 158/175 (90.29%), Postives = 169/175 (96.57%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE+LEG LTAQQTSDKEALEAALDAYERLA+V+ERL+AL+ RVEQAPEL++RLQREL+EAEEASQQLSV  LSDR LDELETLQT+AVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSAL+DARLI+ RLERALADREVSFYQRELS
Sbjct:   49 LEALEGALTAQQTSDKEALEAALDAYERLASVDERLAALDTRVEQAPELLTRLQRELSEAEEASQQLSVAGLSDRRLDELETLQTEAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALSDARLIQLRLERALADREVSFYQRELS 223
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A6I7MZR6_HALSX (Mechanosensitive channel MscK n=2 Tax=root TaxID=1 RepID=A0A6I7MZR6_HALSX)

HSP 1 Score: 262 bits (670), Expect = 1.480e-78
Identity = 150/175 (85.71%), Postives = 167/175 (95.43%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE++E  LT QQ SDK+ALEAAL+AYERL++VNERL+ALE+RVE+APEL+SRLQRELNEAEEA+QQLSV NLSDRPLDELET+Q +AVVELQ+LQSQLAEVNSQLL AQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALND RLI+WRLERALA+REVSF+QRELS
Sbjct:   47 LEAIESELTTQQASDKDALEAALNAYERLSSVNERLAALESRVEEAPELLSRLQRELNEAEEAAQQLSVANLSDRPLDELETMQNEAVVELQRLQSQLAEVNSQLLTAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDPRLIQWRLERALAEREVSFFQRELS 221
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A101D3I9_9GAMM (Mechanosensitive ion channel MscS n=1 Tax=Halomonas sp. 54_146 TaxID=1635257 RepID=A0A101D3I9_9GAMM)

HSP 1 Score: 254 bits (648), Expect = 1.810e-75
Identity = 149/175 (85.14%), Postives = 165/175 (94.29%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE++EG LTAQQTS+KEALEAAL+ YERL  V+ERL+AL+ RVEQAPEL+ RL+R+L+EAEEASQQ SVT+LSDRPLDELETLQT AVVELQQLQSQLA+VNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALL DRQLS+L+DARLI+ RLERALADREVSFYQRELS
Sbjct:   52 LEAIEGELTAQQTSEKEALEAALETYERLGLVDERLAALDTRVEQAPELLVRLKRDLSEAEEASQQRSVTDLSDRPLDELETLQTTAVVELQQLQSQLADVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLTDRQLSSLSDARLIQLRLERALADREVSFYQRELS 226
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A3T1DU07_9GAMM (MscS_porin domain-containing protein n=1 Tax=Halomonas olivaria TaxID=390919 RepID=A0A3T1DU07_9GAMM)

HSP 1 Score: 240 bits (612), Expect = 5.400e-75
Identity = 143/175 (81.71%), Postives = 160/175 (91.43%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE+LE  LT QQ+ DKEALEAA++AYERLA+V+E        VEQAPE+++RLQREL+EAEEASQQL+V +LSDRPLDELETLQT+AVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAE+LRRQHDEREALLADRQLSAL+DARLI+ RLERALA+REV FYQRELS
Sbjct:   18 LEALEEPLTDQQSRDKEALEAAIEAYERLASVDEXXXXXXXXVEQAPEMLARLQRELSEAEEASQQLTVADLSDRPLDELETLQTNAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAENLRRQHDEREALLADRQLSALSDARLIQLRLERALAEREVRFYQRELS 192
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A2A3XEJ2_9GAMM (Mechanosensitive channel MscK n=3 Tax=Halomonas TaxID=2745 RepID=A0A2A3XEJ2_9GAMM)

HSP 1 Score: 249 bits (636), Expect = 8.590e-74
Identity = 147/175 (84.00%), Postives = 163/175 (93.14%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LES EG LT QQ SDK+ALEAALDAYERL +V+ERL+AL++RVEQAPEL++ LQR+L++A+EASQQLSV NLSDRPLDELET+QT AVV+LQQLQSQLAEVNSQLLAAQTLPERAQQ+IAETLQRAESLRRQHDEREALLADRQLSALNDA LI+ RLER LADREVSF QRELS
Sbjct:   51 LESNEGALTNQQASDKKALEAALDAYERLVSVDERLNALDSRVEQAPELLASLQRDLSDAQEASQQLSVANLSDRPLDELETMQTQAVVKLQQLQSQLAEVNSQLLAAQTLPERAQQNIAETLQRAESLRRQHDEREALLADRQLSALNDAALIQLRLERILADREVSFNQRELS 225
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: UPI00178884E9 (mechanosensitive channel MscK n=3 Tax=Halomonas TaxID=2745 RepID=UPI00178884E9)

HSP 1 Score: 216 bits (551), Expect = 4.470e-62
Identity = 132/175 (75.43%), Postives = 149/175 (85.14%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            L++ EG LT QQ  DK+AL AAL AYERL+AV ERLSAL  RVEQAPE    L+R+LN+  + SQQLSVT+L+D PLD LE  QTDAVVELQQLQSQLAEVNSQLL+AQTLPERAQQSI+E LQR E LRRQHDEREALLADRQLSALNDA+LI+ RLERALA+REV++ QRELS
Sbjct:   52 LDAAEGELTPQQGRDKDALAAALQAYERLSAVEERLSALTQRVEQAPEQRQLLERQLNDINDDSQQLSVTDLTDTPLDTLEAQQTDAVVELQQLQSQLAEVNSQLLSAQTLPERAQQSISEALQRIEVLRRQHDEREALLADRQLSALNDAQLIQLRLERALAEREVTYNQRELS 226
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A511US66_9GAMM (Potassium transporter KefA n=2 Tax=Halovibrio variabilis TaxID=31910 RepID=A0A511US66_9GAMM)

HSP 1 Score: 215 bits (548), Expect = 1.080e-61
Identity = 134/176 (76.14%), Postives = 148/176 (84.09%), Query Frame = 1 
Query:    1 LESLEG-GLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            LE++E   LTA+Q SDKEALEAALDAY RLA+V ERL+ALEAR++Q                  SQQLSV +LSDRPLDELETLQT AVVELQQLQSQLAE +SQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALL+DRQLSAL+DARLI+ RLERA+A+REVSFYQRELS
Sbjct:   36 LEAIEADALTAEQASDKEALEAALDAYARLASVTERLAALEARIDQXXXXXXXXXXXXXXXXXESQQLSVADLSDRPLDELETLQTQAVVELQQLQSQLAEASSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLSDRQLSALSDARLIQLRLERAVAEREVSFYQRELS 211
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A455VF85_9GAMM (MscS_porin domain-containing protein n=2 Tax=Halomonas TaxID=2745 RepID=A0A455VF85_9GAMM)

HSP 1 Score: 199 bits (507), Expect = 4.350e-61
Identity = 122/175 (69.71%), Postives = 141/175 (80.57%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            +ESLE   TA Q  D+ AL AAL AYERL A+ ER  ALE RV QAPE + RL+RELN A+E S QLSV NLSD PL+ LE    DAV+ELQQLQSQ+AEVNSQLLAAQTLPERAQQ+I+E LQRAE+LRR+HD R ALLADRQLSA  DA+LI+WRLERA+A++EV+  QRELS
Sbjct:   46 IESLETEPTADQQRDQAALTAALQAYERLQAIEERQQALEQRVSQAPEQLLRLERELNAAQEESLQLSVDNLSDMPLEALEAELADAVIELQQLQSQMAEVNSQLLAAQTLPERAQQAISEALQRAETLRREHDTRAALLADRQLSAREDAQLIQWRLERAVAEQEVTLNQRELS 220
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BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Match: A0A356QKY5_HALSX (Mechanosensitive channel MscK (Fragment) n=1 Tax=Halomonas sp. TaxID=1486246 RepID=A0A356QKY5_HALSX)

HSP 1 Score: 201 bits (510), Expect = 4.540e-60
Identity = 123/175 (70.29%), Postives = 141/175 (80.57%), Query Frame = 1 
Query:    1 LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELISRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAEVNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALNDARLIRWRLERALADREVSFYQRELS 525
            +ESLE   TA Q  D+ AL AAL AYERL A+ ER  ALE RV QAPE + RL+RELN A+E S QLSV NLSD PL+ LE    DAV+ELQQLQSQ+AEVNSQLLAAQTLPERAQQ+I++ LQRAE+LRR+HD R ALLADRQLSA  DA+LI+WRLERALA++EVS  QRELS
Sbjct:   46 IESLETEPTADQQRDQAALTAALQAYERLQAIEERQQALEQRVSQAPEQLLRLERELNAAQEESLQLSVDNLSDMPLEALEAELADAVIELQQLQSQMAEVNSQLLAAQTLPERAQQAISDALQRAETLRREHDTRAALLADRQLSAREDAQLIQWRLERALAEQEVSLNQRELS 220
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig103217.526.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A455U9V9_9GAMM3.070e-8588.00MscS_porin domain-containing protein n=1 Tax=Halom... [more]
A0A7Z0S010_9GAMM1.570e-8090.29Mechanosensitive channel MscK n=34 Tax=Halomonadac... [more]
A0A6I7MZR6_HALSX1.480e-7885.71Mechanosensitive channel MscK n=2 Tax=root TaxID=1... [more]
A0A101D3I9_9GAMM1.810e-7585.14Mechanosensitive ion channel MscS n=1 Tax=Halomona... [more]
A0A3T1DU07_9GAMM5.400e-7581.71MscS_porin domain-containing protein n=1 Tax=Halom... [more]
A0A2A3XEJ2_9GAMM8.590e-7484.00Mechanosensitive channel MscK n=3 Tax=Halomonas Ta... [more]
UPI00178884E94.470e-6275.43mechanosensitive channel MscK n=3 Tax=Halomonas Ta... [more]
A0A511US66_9GAMM1.080e-6176.14Potassium transporter KefA n=2 Tax=Halovibrio vari... [more]
A0A455VF85_9GAMM4.350e-6169.71MscS_porin domain-containing protein n=2 Tax=Halom... [more]
A0A356QKY5_HALSX4.540e-6070.29Mechanosensitive channel MscK (Fragment) n=1 Tax=H... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig103217contigEcto-sp8_S_contig103217:2..526 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeBacteria
Stop0
Start0
Seed ortholog score267.7
Seed ortholog evalue3.8e-69
Seed eggNOG ortholog1504981.KO116_4210
Preferred namekefA
Model size525
KEGG koko:K05802,ko:K06994,ko:K15771,ko:K22051
KEGG TC1.A.23.1.1,1.A.23.1.2,1.A.23.1.3,3.A.1.1.16,3.A.1.1.2
KEGG Pathwayko02010,map02010
KEGG ModuleM00491
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005623,GO:0005886,GO:0006884,GO:0008150,GO:0008361,GO:0009987,GO:0009992,GO:0016020,GO:0016043,GO:0019725,GO:0030104,GO:0032535,GO:0042592,GO:0044464,GO:0048878,GO:0055082,GO:0065007,GO:0065008,GO:0071840,GO:0071944,GO:0090066
Exons1
EggNOG free text desc.COG3264 Small-conductance mechanosensitive channel
EggNOG OGs1MWSA@1224,1RMYY@1236,1XHS8@135619,COG1196@1,COG1196@2,COG3264@1,COG3264@2
Cds size525
COG Functional cat.DM
Best tax levelOceanospirillales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143130.8465953-CDS-Ecto-sp8_S_contig103217:1..5261681143130.8465953-CDS-Ecto-sp8_S_contig103217:1..526Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig103217 2..526 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig103217.526.1prot_Ecto-sp8_S_contig103217.526.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig103217 2..526 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig103217.526.1

>prot_Ecto-sp8_S_contig103217.526.1 ID=prot_Ecto-sp8_S_contig103217.526.1|Name=mRNA_Ecto-sp8_S_contig103217.526.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=175bp
LESLEGGLTAQQTSDKEALEAALDAYERLAAVNERLSALEARVEQAPELI
SRLQRELNEAEEASQQLSVTNLSDRPLDELETLQTDAVVELQQLQSQLAE
VNSQLLAAQTLPERAQQSIAETLQRAESLRRQHDEREALLADRQLSALND
ARLIRWRLERALADREVSFYQRELS
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mRNA from alignment at Ecto-sp8_S_contig103217:2..526-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig103217.526.1 ID=mRNA_Ecto-sp8_S_contig103217.526.1|Name=mRNA_Ecto-sp8_S_contig103217.526.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=525bp|location=Sequence derived from alignment at Ecto-sp8_S_contig103217:2..526- (Ectocarpus species8 EcLAC_412)
CTAGAATCGCTCGAGGGTGGATTAACAGCCCAGCAAACCAGCGACAAGGA
AGCTTTAGAGGCGGCACTGGATGCTTATGAGAGATTAGCTGCCGTCAACG
AGCGATTATCTGCTCTGGAAGCCCGTGTTGAGCAGGCGCCTGAGCTGATA
TCGCGCTTACAGCGAGAGCTAAACGAAGCAGAAGAGGCTTCGCAACAGCT
TTCAGTAACTAATTTAAGCGATCGCCCACTCGATGAGTTAGAAACATTAC
AAACCGATGCCGTGGTCGAACTTCAGCAATTGCAAAGCCAACTGGCGGAA
GTGAACTCCCAACTGCTTGCCGCGCAGACACTCCCTGAGCGTGCTCAGCA
ATCCATCGCAGAGACGTTACAACGAGCAGAGAGTTTACGACGACAACACG
ATGAGCGCGAGGCTTTGTTGGCAGATAGGCAGCTTTCCGCTCTTAATGAT
GCACGTCTAATTCGATGGCGTCTTGAGAGGGCGCTAGCCGATCGCGAAGT
AAGCTTCTATCAGCGCGAATTGAGT
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig103217:2..526-

>mRNA_Ecto-sp8_S_contig103217.526.1 ID=mRNA_Ecto-sp8_S_contig103217.526.1|Name=mRNA_Ecto-sp8_S_contig103217.526.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=525bp|location=Sequence derived from alignment at Ecto-sp8_S_contig103217:2..526- (Ectocarpus species8 EcLAC_412)
CTAGAATCGCTCGAGGGTGGATTAACAGCCCAGCAAACCAGCGACAAGGA
AGCTTTAGAGGCGGCACTGGATGCTTATGAGAGATTAGCTGCCGTCAACG
AGCGATTATCTGCTCTGGAAGCCCGTGTTGAGCAGGCGCCTGAGCTGATA
TCGCGCTTACAGCGAGAGCTAAACGAAGCAGAAGAGGCTTCGCAACAGCT
TTCAGTAACTAATTTAAGCGATCGCCCACTCGATGAGTTAGAAACATTAC
AAACCGATGCCGTGGTCGAACTTCAGCAATTGCAAAGCCAACTGGCGGAA
GTGAACTCCCAACTGCTTGCCGCGCAGACACTCCCTGAGCGTGCTCAGCA
ATCCATCGCAGAGACGTTACAACGAGCAGAGAGTTTACGACGACAACACG
ATGAGCGCGAGGCTTTGTTGGCAGATAGGCAGCTTTCCGCTCTTAATGAT
GCACGTCTAATTCGATGGCGTCTTGAGAGGGCGCTAGCCGATCGCGAAGT
AAGCTTCTATCAGCGCGAATTGAGT
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