mRNA_Ecto-sp8_S_contig9534.31142.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig9534.31142.1
Unique NamemRNA_Ecto-sp8_S_contig9534.31142.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: D8LF73_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LF73_ECTSI)

HSP 1 Score: 201 bits (510), Expect = 1.510e-55
Identity = 102/120 (85.00%), Postives = 105/120 (87.50%), Query Frame = 1 
Query:  172 GPDPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRL-------------SDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPSVLAR 492
            GPDPFSMSEKARARYQAIFCKVDATGTGSL+GKQVIGLF KSGLDK ALAVIWRL             SDLDEDGSL+A+EFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPSVLAR
Sbjct:  269 GPDPFSMSEKARARYQAIFCKVDATGTGSLSGKQVIGLFAKSGLDKAALAVIWRLPNLVLCLWSDGRLSDLDEDGSLAAEEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPSVLAR 388
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A6H5L7C1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L7C1_9PHAE)

HSP 1 Score: 190 bits (483), Expect = 1.430e-52
Identity = 92/101 (91.09%), Postives = 97/101 (96.04%), Query Frame = 1 
Query:  190 MSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPSVLAR 492
            MSEKARARYQAIFCK+DATGTGSL+GKQVIGLF KSGLDKTALAVIWRLSDLDEDGSL+A+EFGIAFHLIFCATQRKLNVP+ELP SLWPAGY PPS LAR
Sbjct:    1 MSEKARARYQAIFCKIDATGTGSLSGKQVIGLFAKSGLDKTALAVIWRLSDLDEDGSLTAEEFGIAFHLIFCATQRKLNVPNELPHSLWPAGYEPPSFLAR 101
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A7S2W1I6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2W1I6_9STRA)

HSP 1 Score: 78.6 bits (192), Expect = 5.340e-13
Identity = 39/92 (42.39%), Postives = 56/92 (60.87%), Query Frame = 1 
Query:  178 DPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSL 453
            DP+ M    ++RY+ +F +  +   G ++GK+ +GLF+KSGLDK  L  IW + D   D  LS  EF +A HLI C +++ L VP  LP SL
Sbjct:  179 DPYIMQPGEQSRYETLFPQYMSKEDGFVHGKEAVGLFSKSGLDKGTLRDIWNMVDDPIDNRLSKLEFAMAMHLIVCISKKGLPVPPSLPMSL 270
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A507CYQ2_9FUNG (Uncharacterized protein n=2 Tax=Synchytrium endobioticum TaxID=286115 RepID=A0A507CYQ2_9FUNG)

HSP 1 Score: 73.2 bits (178), Expect = 3.660e-11
Identity = 38/84 (45.24%), Postives = 54/84 (64.29%), Query Frame = 1 
Query:  202 ARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSL 453
            AR RY+ +F + DA   G L+G +V  ++ +SGLD   L  IW L+D+DEDGSL+ +EF I   LI     R +++PD+LPQ L
Sbjct:  494 ARVRYEDVFNRYDANHEGFLSGDEVRTIWMRSGLDPPTLGEIWALADVDEDGSLTKEEFCIGMFLIDDRL-RGIHMPDQLPQGL 576
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A151Z5X1_9MYCE (EF-hand domain-containing protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z5X1_9MYCE)

HSP 1 Score: 72.0 bits (175), Expect = 8.310e-11
Identity = 35/90 (38.89%), Postives = 55/90 (61.11%), Query Frame = 1 
Query:  190 MSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWP 459
            MS ++RA+Y  ++ K+     G ++G     +  KS L    LA +WRLSD+D DGSL  +EF +A HLI+ A +  +++P +LP+ L P
Sbjct:  307 MSNESRAQYYNVYYKIGGNDGGKVSGSTAKPILMKSQLPTEKLAEVWRLSDIDSDGSLDREEFALAMHLIY-ALKSGISLPTQLPKHLIP 395
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A6F9DQR3_9ASCI (RalBP1-associated Eps domain-containing protein 1-like n=1 Tax=Phallusia mammillata TaxID=59560 RepID=A0A6F9DQR3_9ASCI)

HSP 1 Score: 72.0 bits (175), Expect = 8.970e-11
Identity = 38/94 (40.43%), Postives = 55/94 (58.51%), Query Frame = 1 
Query:  178 DPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWP 459
            DP+ ++ + R  Y   F  + A   G +NG+     FTKS L    L+ IW LSD+D+DG L+ DEF  AFHL+  A +   ++P +LPQ+L P
Sbjct:  222 DPWCITPEQREYYTKQFATMQADLEGKINGQSARNFFTKSKLPILELSHIWELSDMDQDGHLTLDEFCTAFHLVV-ARKNGYDLPSQLPQALVP 314
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A6F9DPZ5_9ASCI (RalBP1-associated Eps domain-containing protein 1-like n=1 Tax=Phallusia mammillata TaxID=59560 RepID=A0A6F9DPZ5_9ASCI)

HSP 1 Score: 72.0 bits (175), Expect = 9.680e-11
Identity = 38/94 (40.43%), Postives = 55/94 (58.51%), Query Frame = 1 
Query:  178 DPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWP 459
            DP+ ++ + R  Y   F  + A   G +NG+     FTKS L    L+ IW LSD+D+DG L+ DEF  AFHL+  A +   ++P +LPQ+L P
Sbjct:  179 DPWCITPEQREYYTKQFATMQADLEGKINGQSARNFFTKSKLPILELSHIWELSDMDQDGHLTLDEFCTAFHLVV-ARKNGYDLPSQLPQALVP 271
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A3G2S4U8_9BASI (EH domain-containing and endocytosis protein 1 n=2 Tax=Malassezia restricta TaxID=76775 RepID=A0A3G2S4U8_9BASI)

HSP 1 Score: 71.6 bits (174), Expect = 1.400e-10
Identity = 39/102 (38.24%), Postives = 60/102 (58.82%), Query Frame = 1 
Query:  178 DPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPSV 483
            D ++++   + RY   F  +D    G ++G  V+  F +SGLD++ LA +W L+D+++ G+L+ DEF +A HLI      K     ELPQ L PA  VPPS+
Sbjct:  287 DGWAITPADKTRYDGFFDSLDTERAGYVDGSVVVPFFLQSGLDESTLAHVWDLTDMNQSGTLTRDEFAVAMHLINDRISGK-----ELPQEL-PASLVPPSM 382
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: UPI0005213699 (intersectin-1-like n=2 Tax=Neognathae TaxID=8825 RepID=UPI0005213699)

HSP 1 Score: 70.5 bits (171), Expect = 2.650e-10
Identity = 38/99 (38.38%), Postives = 58/99 (58.59%), Query Frame = 1 
Query:  184 FSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPS 480
            +++ + +R +Y+ +F   D T +G L G Q   +  +S L +T LA IW LSD+D+DG L+A+EF +A HLI  A   +   P  LP+      Y+PPS
Sbjct:  154 WAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQTQLATIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLLPE------YIPPS 246
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BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Match: A0A5F8HDG6_MONDO (Intersectin 1 n=1 Tax=Monodelphis domestica TaxID=13616 RepID=A0A5F8HDG6_MONDO)

HSP 1 Score: 69.7 bits (169), Expect = 6.050e-10
Identity = 39/99 (39.39%), Postives = 59/99 (59.60%), Query Frame = 1 
Query:  184 FSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSGLDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQSLWPAGYVPPS 480
            +++ + +R +Y+ +F   D T +G L G Q   +  +S L +T LA IW LSD+D+DG L+A+EF +A HLI  A   +      LP +L P  Y+PPS
Sbjct:  178 WAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQTQLATIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQ-----PLPPAL-PPEYIPPS 270
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig9534.31142.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LF73_ECTSI1.510e-5585.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5L7C1_9PHAE1.430e-5291.09Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S2W1I6_9STRA5.340e-1342.39Hypothetical protein (Fragment) n=1 Tax=Eucampia a... [more]
A0A507CYQ2_9FUNG3.660e-1145.24Uncharacterized protein n=2 Tax=Synchytrium endobi... [more]
A0A151Z5X1_9MYCE8.310e-1138.89EF-hand domain-containing protein n=1 Tax=Tieghemo... [more]
A0A6F9DQR3_9ASCI8.970e-1140.43RalBP1-associated Eps domain-containing protein 1-... [more]
A0A6F9DPZ5_9ASCI9.680e-1140.43RalBP1-associated Eps domain-containing protein 1-... [more]
A0A3G2S4U8_9BASI1.400e-1038.24EH domain-containing and endocytosis protein 1 n=2... [more]
UPI00052136992.650e-1038.38intersectin-1-like n=2 Tax=Neognathae TaxID=8825 R... [more]
A0A5F8HDG6_MONDO6.050e-1039.39Intersectin 1 n=1 Tax=Monodelphis domestica TaxID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig9534contigEcto-sp8_S_contig9534:4017..6292 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score203.4
Seed ortholog evalue1.3e-49
Seed eggNOG ortholog2880.D8LF73
Preferred nameENAH
Model size748
KEGG koko:K03099,ko:K05746
KEGG Pathwayko01521,ko01522,ko04010,ko04012,ko04013,ko04014,ko04062,ko04068,ko04072,ko04150,ko04151,ko04320,ko04360,ko04510,ko04540,ko04630,ko04650,ko04660,ko04662,ko04664,ko04714,ko04722,ko04810,ko04910,ko04912,ko04915,ko04917,ko04926,ko05034,ko05160,ko05165,ko05200,ko05205,ko05206,ko05210,ko05211,ko05213,ko05214,ko05215,ko05220,ko05221,ko05223,ko05224,ko05225,ko05226,ko05231,map01521,map01522,map04010,map04012,map04013,map04014,map04062,map04068,map04072,map04150,map04151,map04320,map04360,map04510,map04540,map04630,map04650,map04660,map04662,map04664,map04714,map04722,map04810,map04910,map04912,map04915,map04917,map04926,map05034,map05160,map05165,map05200,map05205,map05206,map05210,map05211,map05213,map05214,map05215,map05220,map05221,map05223,map05224,map05225,map05226,map05231
Hectar predicted targeting categoryother localisation
Exons5
EggNOG free text desc.positive regulation of caveolin-mediated endocytosis
EggNOG OGsKOG1029@1,KOG1029@2759
Cds size747
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145314.0564303-CDS-Ecto-sp8_S_contig9534:4016..42351681145314.0564303-CDS-Ecto-sp8_S_contig9534:4016..4235Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9534 4017..4235 +
1681145314.0715227-CDS-Ecto-sp8_S_contig9534:4636..47491681145314.0715227-CDS-Ecto-sp8_S_contig9534:4636..4749Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9534 4637..4749 +
1681145314.083765-CDS-Ecto-sp8_S_contig9534:5211..53281681145314.083765-CDS-Ecto-sp8_S_contig9534:5211..5328Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9534 5212..5328 +
1681145314.0986094-CDS-Ecto-sp8_S_contig9534:5740..59491681145314.0986094-CDS-Ecto-sp8_S_contig9534:5740..5949Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9534 5741..5949 +
1681145314.1102452-CDS-Ecto-sp8_S_contig9534:6203..62921681145314.1102452-CDS-Ecto-sp8_S_contig9534:6203..6292Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9534 6204..6292 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig9534.31142.1prot_Ecto-sp8_S_contig9534.31142.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig9534 4017..6292 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig9534.31142.1

>prot_Ecto-sp8_S_contig9534.31142.1 ID=prot_Ecto-sp8_S_contig9534.31142.1|Name=mRNA_Ecto-sp8_S_contig9534.31142.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=249bp
MGPENDENGDDESGSVGGLDSTAGTSRGSKWIGSGRSQPSCASSSASSPS
GEEEEDLGPDPFSMSEKARARYQAIFCKVDATGTGSLNGKQVIGLFTKSG
LDKTALAVIWRLSDLDEDGSLSADEFGIAFHLIFCATQRKLNVPDELPQS
LWPAGYVPPSVLARRKEEAEKKRKQEEQERRRREKTVADSSGKSNNDAAA
GTTKKKKKDKSKKIGASKKGTEVAGGGIGNEKTEEDKKKEERELRAARE
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mRNA from alignment at Ecto-sp8_S_contig9534:4017..6292+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig9534.31142.1 ID=mRNA_Ecto-sp8_S_contig9534.31142.1|Name=mRNA_Ecto-sp8_S_contig9534.31142.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=2276bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9534:4017..6292+ (Ectocarpus species8 EcLAC_412)
ATGGGCCCGGAAAATGATGAGAATGGTGATGATGAATCGGGATCGGTTGG
AGGCCTCGACAGCACAGCAGGCACCAGTAGAGGAAGCAAGTGGATAGGAT
CCGGGCGGTCCCAACCCTCTTGTGCCTCTTCGTCAGCATCCTCGCCGTCA
GGCGAGGAGGAGGAGGACTTAGGCCCCGATCCTTTTTCAATGTCGGAAAA
GGCACGGGCTAGATACCAGGTGTGTTATGGAGGAGAGCTGCCTTGAGTTG
CCGGCATCAAGCGTGTTCATGCGTTCCACGCTGTTGCAATGTTTTCGGTC
TACACTTGTACAAGTACTATGAAGGTTCCTACTGTAGTTCTTGTTGGTTG
AACACCCCTTTTCGCGCACACACATTGATCCGGACGGCATAAATCCAAGA
CAATGTTTGATCCGCGCATTATACGTACGTCGAAGGAAGCTGAAGTCTAG
GATCAGGTGATAGGTGGTAAAGCCGATTCTTCAGATTAGCTTCTTTTAAA
AGTATTAGTATTTTCCCGTGCTCGACACACCTCGAGTCAGAAAAATTGCA
GCGCAGATAAATCACTTGTTGGGTCGCCGCATTTATGCTGGTTGAACAAT
ACAACTATACCTTGAAACAGGCGATATTTTGCAAAGTCGACGCCACGGGG
ACTGGATCATTGAACGGAAAGCAGGTTATAGGGTTGTTCACCAAATCAGG
GCTTGATAAGACAGCTTTGGCTGTCATTTGGAGGTGAGAAATTGCGTCAT
ATGTATGTTTGACTGCAAAGAAGTATTGCTGCATCTGATGATTAAAAAAA
AAGAGTGCTTATTTCGTTCAGTTGTTCATTCTCTCTCTGGTCGGCAACGT
GAACGTTTGACTGGCACACAAAGGGATGTGATGTAGACGCTCGGTGTGTT
GTGGTAGGTCCACGAGAACGATCGCCGCACAGCCATTTGGCAGCTCGAGG
GGAGCTCCGAGTGGAAGAGTAACCGTTCTCGAGAAACGCACGCACTCACC
ATTGTAAGCCCTTCTATTGCATAGGTTGCTACAGGGACTCTTCTCGGCCG
ACGCAAAGCAGTGGTCTACGGTCTTCCTTGGGTAAACCGTCCAATACATG
TGTCTTCCGAAGCCATTCACTTGGCCTATCCAACCGATTCAAATTCCGAA
CAAACAGGCTTCTGAATCTTGTGTTATTTTGTGGTCCGATGGAAGGTTGT
CAGATCTAGACGAAGACGGGAGCCTCTCGGCAGACGAGTTCGGGATCGCT
TTCCATCTCATCTTTTGCGCAACCCAACGCAAGCTGAACGTGCCGGATGA
GCTTCCCCAAAGGTTTGTTGGCCAAGAGCTACATGGCGATATTCTTATCT
CTACGGTGGAGTTTCTCCTTTGTCCGAATCTCGCCCGGGCGAACTGCAGT
CAGTCTTCGTCACCCAGAAACGATTGTAGGCGTGTTCATCTGCTTTAGGG
GCTGCGCGCTAGCACTGCAACTGATTCGGAGATGTGGTGCAGTTCTCTCC
GTCGCACTTTAACGCCTGCTCTAGCCTCTTCCCCAAAAGCTCTAGAACGA
TACAAGGAGAGCAGTTCAGTATTGTGGAGCACGGTTCAGTATTGTGGCGC
CCTATGAGGAAGAAACATGGAGTGTGCTTTGCATTAGAGTTATTGCTTGT
GCTCTCGAAACGTCATGCCCACTTGTCTACGGTAGGACCGCACCTTAACC
CTCTCAACCTCCTGGTTTCGGCAGCCTGTGGCCAGCTGGGTATGTACCTC
CGTCGGTCCTCGCTCGGCGTAAGGAGGAGGCGGAGAAAAAACGAAAACAA
GAGGAGCAAGAGCGAAGACGAAGGGAAAAGACAGTAGCCGACAGCAGCGG
GAAGTCAAACAACGATGCCGCTGCAGGCACCACGAAGAAGAAGAAAAAGG
ACAAGAGCAAGAAGATCGGGGCAAGCAAGAAAGGTGACGACGAGAAATGA
CCAATGGGGCGTGTCAGCAGATAAAGCAGCGATTGTCCTATCGCAGAAAG
GGAGCCGTTTTCGAAATGGTGCCAAATGCATGTGGTCTGCCCTTCTGTGG
GTTTGTTTTGCTGTAGCTGCTCAGTTCCCGGTGCTTGGTTTCATCTCCCT
GATTTCGTGTTCGTTCAACGCTGAGCGCACTTTCTTCGAGCGTGGTCCAA
CTGCCGCGCGGATAAATCTGTGACGCCTACGGAACAGGCACTGAGGTAGC
TGGCGGAGGAATCGGCAACGAGAAAACGGAGGAAGACAAAAAGAAGGAGG
AGCGTGAGCTAAGAGCCGCGAGGGAG
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig9534:4017..6292+

>mRNA_Ecto-sp8_S_contig9534.31142.1 ID=mRNA_Ecto-sp8_S_contig9534.31142.1|Name=mRNA_Ecto-sp8_S_contig9534.31142.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=747bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9534:4017..6292+ (Ectocarpus species8 EcLAC_412)
ATGGGCCCGGAAAATGATGAGAATGGTGATGATGAATCGGGATCGGTTGG
AGGCCTCGACAGCACAGCAGGCACCAGTAGAGGAAGCAAGTGGATAGGAT
CCGGGCGGTCCCAACCCTCTTGTGCCTCTTCGTCAGCATCCTCGCCGTCA
GGCGAGGAGGAGGAGGACTTAGGCCCCGATCCTTTTTCAATGTCGGAAAA
GGCACGGGCTAGATACCAGGCGATATTTTGCAAAGTCGACGCCACGGGGA
CTGGATCATTGAACGGAAAGCAGGTTATAGGGTTGTTCACCAAATCAGGG
CTTGATAAGACAGCTTTGGCTGTCATTTGGAGGTTGTCAGATCTAGACGA
AGACGGGAGCCTCTCGGCAGACGAGTTCGGGATCGCTTTCCATCTCATCT
TTTGCGCAACCCAACGCAAGCTGAACGTGCCGGATGAGCTTCCCCAAAGC
CTGTGGCCAGCTGGGTATGTACCTCCGTCGGTCCTCGCTCGGCGTAAGGA
GGAGGCGGAGAAAAAACGAAAACAAGAGGAGCAAGAGCGAAGACGAAGGG
AAAAGACAGTAGCCGACAGCAGCGGGAAGTCAAACAACGATGCCGCTGCA
GGCACCACGAAGAAGAAGAAAAAGGACAAGAGCAAGAAGATCGGGGCAAG
CAAGAAAGGCACTGAGGTAGCTGGCGGAGGAATCGGCAACGAGAAAACGG
AGGAAGACAAAAAGAAGGAGGAGCGTGAGCTAAGAGCCGCGAGGGAG
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