mRNA_Ecto-sp8_S_contig9211.30590.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig9211.30590.1
Unique NamemRNA_Ecto-sp8_S_contig9211.30590.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D8LN45_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LN45_ECTSI)

HSP 1 Score: 290 bits (742), Expect = 2.110e-98
Identity = 150/158 (94.94%), Postives = 154/158 (97.47%), Query Frame = 1 
Query:    1 MKACGSRRNGTDSLYALALCRTTGAFFLASHVSSVPSPEGDFCLALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            MKACGSRRNGTDS YALALCRTTGAF LAS VSSVPSPEG+FCLALDLQ+NEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGH QSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLC+TE+
Sbjct:    1 MKACGSRRNGTDSPYALALCRTTGAFILASQVSSVPSPEGNFCLALDLQENEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHIQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCVTEM 158
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: A0A6H5K249_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K249_9PHAE)

HSP 1 Score: 234 bits (598), Expect = 4.000e-76
Identity = 122/137 (89.05%), Postives = 128/137 (93.43%), Query Frame = 1 
Query:   64 TTGAFFLASHVSSVPSPEGDFCLALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            TTGAFFLA+  SSVPS EG FCL LDLQ++EDVTFSD LGFV NNGIL+IQF LLLA+FVKPLMIPQGHTQSGGAVYRRLVMRNVACTI ITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTE+
Sbjct:   52 TTGAFFLAAQASSVPSLEGTFCLTLDLQEDEDVTFSDALGFVINNGILIIQFALLLAIFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIAITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEL 188
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: A0A6H5JPA3_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPA3_9PHAE)

HSP 1 Score: 235 bits (599), Expect = 2.520e-74
Identity = 122/137 (89.05%), Postives = 128/137 (93.43%), Query Frame = 1 
Query:   64 TTGAFFLASHVSSVPSPEGDFCLALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            TTGA FLA+  SSVPS EG FCL LDL+++EDVTFSDTLGFV NNGIL+IQF LLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTI ITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTE+
Sbjct:  153 TTGALFLAAQASSVPSLEGTFCLTLDLKEDEDVTFSDTLGFVINNGILIIQFALLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIAITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEI 289
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D8LN53_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LN53_ECTSI)

HSP 1 Score: 120 bits (301), Expect = 4.240e-32
Identity = 59/99 (59.60%), Postives = 77/99 (77.78%), Query Frame = 1 
Query:  178 LGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            +GF  NN +L +QFVLLLAMF+KP+++PQG TQSGGAVYRR+V+RN+ CT+GI   Y +TTV+VVL  ++T   + KAA L  + P+ NI VTLCLTEV
Sbjct:    2 IGFASNNALLTVQFVLLLAMFIKPMLLPQGGTQSGGAVYRRVVVRNIVCTVGIACAYVITTVVVVLAHVSTPSDTNKAAVLGHITPSINICVTLCLTEV 100
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: A0A6H5JPA0_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JPA0_9PHAE)

HSP 1 Score: 124 bits (312), Expect = 6.580e-32
Identity = 63/120 (52.50%), Postives = 85/120 (70.83%), Query Frame = 1 
Query:  115 EGDFCLALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            EG  C     ++ E     D  GF  NN +L +Q+VLLLAMF+KP+++  G+TQSGGAVYRR+V+RN+ CTIGI   Y +TTV+VVL  L++ P + KAA + D++P+ NI VTLCLTEV
Sbjct:  124 EGTACFNSYGEETETREVGDVAGFAGNNVLLTVQYVLLLAMFIKPMLVSHGNTQSGGAVYRRVVIRNIVCTIGIACAYVITTVVVVLALLSSRPDTDKAAAVGDIVPSINICVTLCLTEV 243
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D7G2C9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G2C9_ECTSI)

HSP 1 Score: 102 bits (253), Expect = 7.590e-24
Identity = 58/136 (42.65%), Postives = 83/136 (61.03%), Query Frame = 1 
Query:   73 AFFLASHVSSVPSPEGDFCL--ALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            A  L +       P G FC    +    +E  T ++   F  NN +LV+QF+LLL +FV+P+++     QSGGAVYRR+V+RNV CT GI  +YA+TT++VV+  L  +  S K+AQ+ D+   AN   TLCLTE+
Sbjct:   25 ALMLGTRFPGGTDPAGTFCYNGIVPTGASELTTSNNAQSFGINNVLLVVQFILLLGLFVQPMLVRVEGAQSGGAVYRRVVLRNVFCTAGIVISYAITTLVVVIALLRESVESNKSAQIRDVALAANSLGTLCLTEI 160
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: A0A6H5JY97_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JY97_9PHAE)

HSP 1 Score: 97.1 bits (240), Expect = 7.290e-21
Identity = 53/118 (44.92%), Postives = 76/118 (64.41%), Query Frame = 1 
Query:  124 FCL-ALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIFVTLCLTEV 474
            FC+    + D+ D    + LG+  N G LVIQFVLLL +FV+PL++    +QSG A+YR +V++N+ CTI +   YAVTTV+V+   +  +  S  A+  AD+ P  N FVTLCLTE+
Sbjct:  193 FCVNGYHMDDDTDEVNDNVLGYGSNMGSLVIQFVLLLILFVRPLLVRLEGSQSGEAMYRMVVIKNITCTIAVVTAYAVTTVVVIAALIRQSNDSNTASLFADITPLVNQFVTLCLTEI 310
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D8LN54_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LN54_ECTSI)

HSP 1 Score: 90.1 bits (222), Expect = 3.450e-19
Identity = 45/96 (46.88%), Postives = 61/96 (63.54%), Query Frame = 1 
Query:   73 AFFLASHVSSVPSPEGDFCLALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTT 360
            A FL        + EG  C     ++ +     D  GF  NN +L +Q+VLLLAMF+KP+++ QG+TQSGGAVYRR+V+RN+ CTIGI   Y +TT
Sbjct:  100 ATFLGLTFPGEVNSEGTACFNSYREETDTAEVGDVAGFASNNVLLTVQYVLLLAMFIKPMLVSQGNTQSGGAVYRRVVIRNIVCTIGIACAYVITT 195
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D7G4H2_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G4H2_ECTSI)

HSP 1 Score: 78.2 bits (191), Expect = 8.780e-16
Identity = 44/80 (55.00%), Postives = 55/80 (68.75%), Query Frame = 1 
Query:  178 LGFVFNNGI-LVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAA 414
            +GF+ NN + LVIQ  LLLAMFVK +MI QG TQSG AVYRRLV R++ CT+ +   Y  TT +VVL  L   PG+ + A
Sbjct:    1 MGFMTNNNMMLVIQLTLLLAMFVKLIMISQGSTQSGRAVYRRLVARSIVCTVAVVLGYTTTTAVVVLAVLAAFPGNNQIA 80
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BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Match: D8LG96_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LG96_ECTSI)

HSP 1 Score: 75.9 bits (185), Expect = 2.850e-13
Identity = 40/91 (43.96%), Postives = 60/91 (65.93%), Query Frame = 1 
Query:  115 EGDFCL-ALDLQDNEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRRLVMRNVACTIGITFTYAVTTVIVVLGFL 384
            E  FC+   ++ D+ D    +  G+  N G L+IQFVLLL +FV+PL++    +QSG A+YRR+V++N+ CTI +   YAVTTV+V+   L
Sbjct:  187 ERTFCINGYNMDDDADEVHENIRGYGSNMGSLIIQFVLLLILFVRPLLVRLEGSQSGEAIYRRVVIKNITCTIAVVTAYAVTTVVVIAALL 277
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig9211.30590.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 13
Match NameE-valueIdentityDescription
D8LN45_ECTSI2.110e-9894.94Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5K249_9PHAE4.000e-7689.05Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JPA3_9PHAE2.520e-7489.05Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LN53_ECTSI4.240e-3259.60Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JPA0_9PHAE6.580e-3252.50Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G2C9_ECTSI7.590e-2442.65Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JY97_9PHAE7.290e-2144.92Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LN54_ECTSI3.450e-1946.88Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G4H2_ECTSI8.780e-1655.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D8LG96_ECTSI2.850e-1343.96Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig9211contigEcto-sp8_S_contig9211:21..2554 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Model size480
Hectar predicted targeting categoryother localisation
Exons4
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-05_003440.1
Cds size480
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145273.2105007-CDS-Ecto-sp8_S_contig9211:20..921681145273.2105007-CDS-Ecto-sp8_S_contig9211:20..92Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9211 21..92 -
1681145273.2221432-CDS-Ecto-sp8_S_contig9211:1004..11961681145273.2221432-CDS-Ecto-sp8_S_contig9211:1004..1196Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9211 1005..1196 -
1681145273.2327535-CDS-Ecto-sp8_S_contig9211:1528..16271681145273.2327535-CDS-Ecto-sp8_S_contig9211:1528..1627Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9211 1529..1627 -
1681145273.2443466-CDS-Ecto-sp8_S_contig9211:2437..25541681145273.2443466-CDS-Ecto-sp8_S_contig9211:2437..2554Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9211 2438..2554 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig9211.30590.1prot_Ecto-sp8_S_contig9211.30590.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig9211 21..2554 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig9211.30590.1

>prot_Ecto-sp8_S_contig9211.30590.1 ID=prot_Ecto-sp8_S_contig9211.30590.1|Name=mRNA_Ecto-sp8_S_contig9211.30590.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=160bp
MKACGSRRNGTDSLYALALCRTTGAFFLASHVSSVPSPEGDFCLALDLQD
NEDVTFSDTLGFVFNNGILVIQFVLLLAMFVKPLMIPQGHTQSGGAVYRR
LVMRNVACTIGITFTYAVTTVIVVLGFLTTAPGSIKAAQLADLLPTANIF
VTLCLTEVR*
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mRNA from alignment at Ecto-sp8_S_contig9211:21..2554-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig9211.30590.1 ID=mRNA_Ecto-sp8_S_contig9211.30590.1|Name=mRNA_Ecto-sp8_S_contig9211.30590.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=2534bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9211:21..2554- (Ectocarpus species8 EcLAC_412)
ATGAAGGCCTGCGGCTCACGTCGCAACGGAACCGACTCCCTCTATGCTCT
TGCTTTGTGCAGGACGACGGGTGCATTCTTTTTGGCATCCCACGTTTCTA
GCGTGCCGAGCCCAGAGGTAAGCACGTGTCAAGTCGTCGATTGCCGCAAT
ACACACCTCGCAATCCCGCTTCGGGCCGACTCAAAACTCTTTGTCCTGCT
TGCACCCCTTTTTGTGGGAGCAAAGAGTTGCGAGAAAAATTTATCTCACG
TACGCTCTTTATGGGCGCAAAACGTTTAACCTGGAAAATTTTGCTTTCGA
CAAAGATCGCGCGATCTGGAGTAAGAGTATTCAAACTATCCTCGCGACAA
ACTTGTCGAGCAAAAGGTTTTGCGGCGAGACTTCCATCAATCGGCGTCAA
GCGCCTGCCCCATCCCACACGCGCAGACCCTCTCCCGCCACACACGCGCG
CACGATCCAAACGATAGATGAACGGCCAAGTCACAATCCTTGAACGACGA
GTTGGGATTGCCTGCGATGGCCGCGGCTGTGTAGAAAACGTTCTGCGTCA
ACAATTTGTATTGGATGTGAGCATGCTTGAGATCTTCCTGGCTTCGATTG
GAAGACACAACGCCCGGCTTTGTGCCGCCAAATCATTTTCCAAGCTTTTG
TTGCACGCTCTGGCCGGTTTTGCGGTGTGGTGTGAAGCGGCGTACCCGCG
TCTCGCCGTTGCTATATAAACAAAAAGGGACCACGCTTCCGCATTCCACC
AGTCCCGTGAGTAAAACTTCATTCGCCCCTTTATCAGCGTGTATTGTAGT
ATAAATGGTTTTTGCAAAAGTTCCTGACCAAAAGTTGCCAAAAAAGGTTG
ATATTTTCTCGCAACTTTTATGCTCCCACAAGAAAGCGGTGTTACGGCTT
GAATAATGCTCCCACGTTCTTTTCCAGGGGGATTTCTGCTTGGCTCTAGA
TCTACAAGACAACGAGGACGTGACGTTTTCGGACACGCTCGGATTCGTGT
TTAACAACGGGATACTAGTCATCCAGGTGAGAACTGACAGGTCGACGACG
TAGTCGCCTTGGGTGGAACAAGACCTGCTTACATGGTACGTCTTGAACGG
ATTGTTGTCGGGGAAAGGCTGGCGCGACCGCCAAGAACGACTGCATTTCA
CTGGGGATGACGAAACGGGGCAGGGGGGTCGGGCCCAGGAATGGGTATTC
GCAGAGCTGAATTGTTCCATCTCTGCCGTAGGCCCACGTGCGTAGGTGGA
GTACGGCGTTATAGTGGGGCACAGGCTCCTACTGGCCTGTCGACAACTGA
CCGCACCAAATCGTTTTGATTGGTATTGCCTCGTTCCGCCGCACGTTCGT
CGCGTTAGTTCGTGCTACTGCTGGCGATGTTCGTGAAGCCGCTGATGATC
CCCCAAGGCCATACCCAGTCAGGCGGGGCGGTGTACCGTCGGCTGGTGAT
GCGAAACGTTGCATGCACGATCGGCATCACCTTCACCTACGCCGTCACAA
CCGTAATTGTGGTATTGGGCTTTCTTACCACTGCTCCCGGGAGCATCAAG
GTGTGGAAGAGCGGGGCGGGTGTCGTACAGTGCCTCGTGCATGCCAAGCT
ACGTACAGCGCGTGATATCGCGAGTACTCAGGCCCGCCTTTGCTATTAGC
ACTGTTGTGACGTTGCGCCCCCATCACCCCGCGTAGAGCAATCCGCGTCG
TAGTACGAGAACTAGCGATACCGGACATGCAACCCTGGGACAAGAAACAA
CGTCGGGAACGTACGGATGTTTAGATTGCTTCGAAGTCATGTGAAGTACA
CGTCGGAAATCATGCTGCCCTCTTGCTACATATGGGTGAGCAGAGCGCTG
CAACACATACAGTACCGTGGGTTCTTGATGTGAAGACCTGGTCGCTCGTG
GCAATTGTTACTCACGGACATTGAGGCCATTTGTTGTCCAGCAATGTGGC
TCTGTGGAGGTTCGTTAAGCACGCGATGTTTGAACGCACCCTCAGCTCGA
CATCCGGTTAGCACTCGGGAACATAGCTATAGCACAAATGCCGAGTCGGC
GCGAATATGAAGCGTGCGCTCTCTTGCTGGGATTTGAGAGTAAGAACCGG
TGTTTCGTTCGTCGCAGGTTGGTCAGCTGTTGATTGGACAAGCAGAAAGA
AGCACGCACTGTCTGTAAGACAAGTTGACTGTCACGAATGAGCGGTGCGT
CGAACCAATCCCTACAGAAGGTCCATTGCCGGCATGGATGGTCATCGCCT
TGCCTCTGGGTGCACCCGGAGCCCTGCCCAACGCTTATTATATCTCCCAG
AGCCCCAACACCCAGGGTTGCAGCTCTTGTCTGTGGAGGCGGTTCAAGGC
AGTGATGGTGCATGCCACCAGCGGGTTATTCTCGGTTGTGCGTGTGATAC
CCAACATGGATCTGACATACGTTTTCTGTTTTGTGCTTCCGTCGACATCA
ACCCTACGGCAGGCGGCTCAACTCGCCGACCTCCTGCCAACGGCAAATAT
TTTCGTGACCCTCTGCCTGACTGAGGTGCGTTAG
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig9211:21..2554-

>mRNA_Ecto-sp8_S_contig9211.30590.1 ID=mRNA_Ecto-sp8_S_contig9211.30590.1|Name=mRNA_Ecto-sp8_S_contig9211.30590.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=480bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9211:21..2554- (Ectocarpus species8 EcLAC_412)
ATGAAGGCCTGCGGCTCACGTCGCAACGGAACCGACTCCCTCTATGCTCT
TGCTTTGTGCAGGACGACGGGTGCATTCTTTTTGGCATCCCACGTTTCTA
GCGTGCCGAGCCCAGAGGGGGATTTCTGCTTGGCTCTAGATCTACAAGAC
AACGAGGACGTGACGTTTTCGGACACGCTCGGATTCGTGTTTAACAACGG
GATACTAGTCATCCAGTTCGTGCTACTGCTGGCGATGTTCGTGAAGCCGC
TGATGATCCCCCAAGGCCATACCCAGTCAGGCGGGGCGGTGTACCGTCGG
CTGGTGATGCGAAACGTTGCATGCACGATCGGCATCACCTTCACCTACGC
CGTCACAACCGTAATTGTGGTATTGGGCTTTCTTACCACTGCTCCCGGGA
GCATCAAGGCGGCTCAACTCGCCGACCTCCTGCCAACGGCAAATATTTTC
GTGACCCTCTGCCTGACTGAGGTGCGTTAG
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