mRNA_Ecto-sp8_S_contig9134.30456.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig9134.30456.1
Unique NamemRNA_Ecto-sp8_S_contig9134.30456.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A6H5JE18_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JE18_9PHAE)

HSP 1 Score: 196 bits (499), Expect = 3.190e-58
Identity = 101/115 (87.83%), Postives = 104/115 (90.43%), Query Frame = 1 
Query:    1 GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSA 345
            GADA+RVDPAEKCGPLHVAAAGGHCSLV DLLIGGAHPNARDLL RTPL+RAAYM QDMVVAVLTDMPSTDKDILD  EL+PLMVA  RGHLST KTLLNAGADVSIR  H NSA
Sbjct:  113 GADADRVDPAEKCGPLHVAAAGGHCSLVGDLLIGGAHPNARDLLGRTPLYRAAYMRQDMVVAVLTDMPSTDKDILDYRELTPLMVALQRGHLSTNKTLLNAGADVSIRPRHENSA 227
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: D8LBP9_ECTSI (Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBP9_ECTSI)

HSP 1 Score: 144 bits (363), Expect = 2.070e-37
Identity = 80/114 (70.18%), Postives = 81/114 (71.05%), Query Frame = 1 
Query:    1 GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNS 342
            GADANRVDPA                           PNARDLL RTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKS LSPL VAS RGHLSTVKTLLNAGADVS+RT H NS
Sbjct:  126 GADANRVDPAXXXXXXXXXXXXXXXXXXXXXXXXXXXPNARDLLGRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSGLSPLTVASQRGHLSTVKTLLNAGADVSMRTGHRNS 239
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A6H5JY41_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JY41_9PHAE)

HSP 1 Score: 104 bits (259), Expect = 8.720e-23
Identity = 58/120 (48.33%), Postives = 71/120 (59.17%), Query Frame = 1 
Query:    1 GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSALHWAA 360
            GAD +RVDP  +  P HVAA GGH  LV + L GGA PN+R  + RTPLH A  +G +  V+ L    +T  D LD    SPLM+AS  GH STV+ LL AGAD + R +      H AA
Sbjct:  128 GADNHRVDPVNRYTPFHVAAEGGHDDLVDNYLTGGACPNSRSRVGRTPLHLADGLGNNRDVSALLGDANTHMDALDNDGFSPLMLASRNGHRSTVEVLLRAGADTTRRATDSGDFSHMAA 247
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: D7FLL7_ECTSI (Ankyrin repeat protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FLL7_ECTSI)

HSP 1 Score: 81.3 bits (199), Expect = 1.170e-14
Identity = 51/110 (46.36%), Postives = 64/110 (58.18%), Query Frame = 1 
Query:    1 GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDI-LDKSEL-SPLMVASLRGHLSTVKTLLNAGADVSIR 324
            G+D N  DP  KC  L  A  G H   V DLLIGGA PN RD     PL  AA   ++ VV+ L  +   D ++ L K+   +PLM+A  RGHL+ V+TLL AGAD S+R
Sbjct:  128 GSDVNFRDPVAKCSVLSQAIQGSHTQFVDDLLIGGARPNIRDDCGMAPLQAAALFAEEGVVSALL-LAKADVNVGLGKTMYETPLMLAVRRGHLAIVETLLEAGADCSLR 236
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A3Q1GZA5_ANATE (Ankyrin repeat domain 16 n=5 Tax=Anabas testudineus TaxID=64144 RepID=A0A3Q1GZA5_ANATE)

HSP 1 Score: 62.4 bits (150), Expect = 3.410e-8
Identity = 45/120 (37.50%), Postives = 61/120 (50.83%), Query Frame = 1 
Query:   22 DPAEKCG--PLHVAAAGGHCSLVSDLLIGG-AHPNARDLLRRTPLHRAAYMGQDMVVAVLT-----DMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSALHWA 357
            D  + CG  PL  A   GH S+   LL    A P A D+L   P+H+ A  GQD  +  L      D+     DI    +L+ L  A+  GH ST+K+LL  GAD+ +R + G +ALH A
Sbjct:  195 DGTDSCGVTPLMDAVRNGHISVARLLLDKHQASPTAADILGSQPVHQVAVTGQDEALRFLVQDLGVDVNQRATDI----QLTALHYAAKEGHTSTIKSLLELGADLHVRDNKGRTALHMA 310
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A6H5KNQ1_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNQ1_9PHAE)

HSP 1 Score: 61.2 bits (147), Expect = 7.410e-8
Identity = 45/120 (37.50%), Postives = 54/120 (45.00%), Query Frame = 1 
Query:    1 GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSALHWAA 360
            GAD N  DP  KC  L  A  G H  LV+DL IGGA PN RD                            D  +   + ++  M+A  RGHL+ V+TLL AGAD S+R     S L  AA
Sbjct:  128 GADVNFRDPVAKCSVLSQAIQGSHTQLVNDLPIGGARPNIRD----------------------------DCGLGKTTYVTTFMLAVRRGHLAIVETLLEAGADCSLRQWADYSPLDVAA 219
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A8H4CEI9_COLGL (Ankyrin repeat domain-containing protein 46 n=1 Tax=Colletotrichum gloeosporioides TaxID=474922 RepID=A0A8H4CEI9_COLGL)

HSP 1 Score: 57.8 bits (138), Expect = 2.200e-7
Identity = 33/90 (36.67%), Postives = 50/90 (55.56%), Query Frame = 1 
Query:   46 LHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLNAGADV 315
            +H AA GG   ++  L   GA+ NA D L+RTPLH AA+ G   VV  L +   +D ++ D    + L +A L GH+ T   L++ G ++
Sbjct:   59 IHCAAIGGSLKILRILEESGANINALDALQRTPLHWAAFRGNQAVVEYLWER--SDTELRDSDGRTALHLAKLSGHVQTANWLISRGGNI 146
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: Q0D1C3_ASPTN (Uncharacterized protein n=2 Tax=Aspergillus terreus TaxID=33178 RepID=Q0D1C3_ASPTN)

HSP 1 Score: 58.5 bits (140), Expect = 4.140e-7
Identity = 42/111 (37.84%), Postives = 61/111 (54.95%), Query Frame = 1 
Query:   43 PLHVAAAGGHCSLVSDLLIGGAHP---NARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHL---STVKTLLNAGADVSIRTSHGNSALHWA 357
            PLH AA  G   LV  LL   A+P   N +D   R  +H AA   +  VV +L    + D D+ D S  +PLM+A+   +    + ++ LL  GADVS+++S G +ALH+A
Sbjct:   10 PLHEAAREGKTQLVESLL--SANPKLANVKDDDDRLAIHWAAAFNRLPVVELLVSTKNFDPDVEDGSGWTPLMIAASLKNAEGDAIIELLLRKGADVSVKSSSGQNALHFA 118
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: A0A662XDT4_9STRA (Uncharacterized protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XDT4_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 4.930e-7
Identity = 46/127 (36.22%), Postives = 63/127 (49.61%), Query Frame = 1 
Query:   25 PAEKCGP-LHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMP--STDK---------------DILDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSALH 351
            PA K G  L +A    H  LV +L+  G +PNA D    + LH AA  GQ  VV +L +M   STD                D  D    +PLM A+  GH++ ++ LL  GA++S+ +  G SALH
Sbjct:  458 PAYKYGQELRLACGRNHLDLVRELISRGCNPNAGDGSGWSALHYAADYGQLAVVDLLAEMTVASTDGEGGSDGKISDQTLEIDARDAHGWTPLMCAAANGHVAVLQRLLALGANLSVASIEGRSALH 584
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BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Match: W9X0H0_9EURO (Uncharacterized protein n=2 Tax=Cladophialophora TaxID=82105 RepID=W9X0H0_9EURO)

HSP 1 Score: 58.9 bits (141), Expect = 6.970e-7
Identity = 49/133 (36.84%), Postives = 66/133 (49.62%), Query Frame = 1 
Query:    1 GADANRVDPAEKCG-----PLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLHRAAYMGQDMVVAVLTDMPSTDKDI-------LDKSELSPLMVASLRGHLSTVKTLLNAGADVSIRTSHGNSALHWAAR 363
            GAD N++     C      PL  AA+ G+ +++  L+  GA   ARD    T L RAA  G+D +V  L + P  D DI        D+   +PLM+A   GH STV+ LL  GA+  +    GN  LH A R
Sbjct:  296 GADPNQLSSNIDCEKPIYLPLCYAASTGNAAVMELLIKKGADIRARDSSGDTVLLRAARYGKDGLVKFLLNAPH-DADIEVRRADTTDRKGFTPLMMAVHYGHPSTVRLLLEKGANREVVDETGNPLLHVAIR 427
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig9134.30456.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5JE18_9PHAE3.190e-5887.83Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D8LBP9_ECTSI2.070e-3770.18Similar to ankyrin 2,3/unc44 n=1 Tax=Ectocarpus si... [more]
A0A6H5JY41_9PHAE8.720e-2348.33Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FLL7_ECTSI1.170e-1446.36Ankyrin repeat protein n=1 Tax=Ectocarpus siliculo... [more]
A0A3Q1GZA5_ANATE3.410e-837.50Ankyrin repeat domain 16 n=5 Tax=Anabas testudineu... [more]
A0A6H5KNQ1_9PHAE7.410e-837.50Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A8H4CEI9_COLGL2.200e-736.67Ankyrin repeat domain-containing protein 46 n=1 Ta... [more]
Q0D1C3_ASPTN4.140e-737.84Uncharacterized protein n=2 Tax=Aspergillus terreu... [more]
A0A662XDT4_9STRA4.930e-736.22Uncharacterized protein n=1 Tax=Nothophytophthora ... [more]
W9X0H0_9EURO6.970e-736.84Uncharacterized protein n=2 Tax=Cladophialophora T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig9134contigEcto-sp8_S_contig9134:6444..6997 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score145.6
Seed ortholog evalue2.3e-32
Seed eggNOG ortholog2880.D8LBP9
Model size555
KEGG koko:K21440
Hectar predicted targeting categoryother localisation
GOsGO:0001508,GO:0002027,GO:0002262,GO:0002376,GO:0002520,GO:0003008,GO:0003012,GO:0003013,GO:0003015,GO:0003205,GO:0003230,GO:0003279,GO:0003283,GO:0003674,GO:0005198,GO:0005200,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005783,GO:0005829,GO:0005856,GO:0005886,GO:0005911,GO:0005938,GO:0006725,GO:0006778,GO:0006779,GO:0006807,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0006887,GO:0006888,GO:0006928,GO:0006936,GO:0006937,GO:0006941,GO:0006942,GO:0006996,GO:0007009,GO:0007010,GO:0007154,GO:0007163,GO:0007165,GO:0007267,GO:0007275,GO:0007507,GO:0008015,GO:0008016,GO:0008092,GO:0008093,GO:0008104,GO:0008150,GO:0008152,GO:0009058,GO:0009893,GO:0009987,GO:0010256,GO:0010468,GO:0010522,GO:0010604,GO:0010628,GO:0010638,GO:0010646,GO:0010880,GO:0010881,GO:0010882,GO:0010959,GO:0012505,GO:0014704,GO:0014731,GO:0015672,GO:0016020,GO:0016043,GO:0016192,GO:0016323,GO:0016528,GO:0016529,GO:0018130,GO:0019222,GO:0019438,GO:0019722,GO:0019725,GO:0019899,GO:0019900,GO:0019901,GO:0019932,GO:0022898,GO:0023051,GO:0023052,GO:0030001,GO:0030003,GO:0030011,GO:0030016,GO:0030017,GO:0030018,GO:0030029,GO:0030048,GO:0030054,GO:0030097,GO:0030099,GO:0030154,GO:0030218,GO:0030315,GO:0030424,GO:0030507,GO:0030673,GO:0030674,GO:0030863,GO:0031252,GO:0031253,GO:0031256,GO:0031430,GO:0031647,GO:0031672,GO:0031674,GO:0032409,GO:0032411,GO:0032412,GO:0032414,GO:0032501,GO:0032502,GO:0032589,GO:0032879,GO:0032940,GO:0032970,GO:0033013,GO:0033014,GO:0033036,GO:0033043,GO:0033267,GO:0033292,GO:0033365,GO:0034101,GO:0034394,GO:0034613,GO:0034641,GO:0034762,GO:0034764,GO:0034765,GO:0034767,GO:0035088,GO:0035090,GO:0035556,GO:0035637,GO:0036309,GO:0036371,GO:0042383,GO:0042391,GO:0042592,GO:0042692,GO:0042995,GO:0043005,GO:0043034,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043266,GO:0043268,GO:0043269,GO:0043270,GO:0043292,GO:0044057,GO:0044093,GO:0044237,GO:0044249,GO:0044271,GO:0044291,GO:0044304,GO:0044325,GO:0044422,GO:0044424,GO:0044425,GO:0044444,GO:0044448,GO:0044449,GO:0044456,GO:0044459,GO:0044463,GO:0044464,GO:0045197,GO:0045199,GO:0045202,GO:0045211,GO:0046483,GO:0046903,GO:0046907,GO:0048193,GO:0048468,GO:0048513,GO:0048518,GO:0048522,GO:0048534,GO:0048731,GO:0048821,GO:0048856,GO:0048869,GO:0048872,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050821,GO:0050896,GO:0051049,GO:0051050,GO:0051117,GO:0051128,GO:0051130,GO:0051179,GO:0051186,GO:0051188,GO:0051234,GO:0051239,GO:0051270,GO:0051279,GO:0051282,GO:0051641,GO:0051649,GO:0051716,GO:0051899,GO:0051924,GO:0051928,GO:0055001,GO:0055065,GO:0055072,GO:0055074,GO:0055076,GO:0055080,GO:0055082,GO:0055117,GO:0060047,GO:0060048,GO:0060090,GO:0060255,GO:0060306,GO:0060307,GO:0060341,GO:0061024,GO:0061061,GO:0061245,GO:0061337,GO:0061515,GO:0065007,GO:0065008,GO:0065009,GO:0070252,GO:0070296,GO:0070727,GO:0070838,GO:0070972,GO:0071704,GO:0071840,GO:0071944,GO:0072359,GO:0072503,GO:0072507,GO:0072511,GO:0072657,GO:0072659,GO:0086001,GO:0086002,GO:0086003,GO:0086004,GO:0086005,GO:0086010,GO:0086012,GO:0086014,GO:0086015,GO:0086018,GO:0086019,GO:0086026,GO:0086046,GO:0086065,GO:0086066,GO:0086070,GO:0086091,GO:0090257,GO:0097060,GO:0097458,GO:0098590,GO:0098771,GO:0098794,GO:0098900,GO:0098901,GO:0098907,GO:0098910,GO:0099080,GO:0099081,GO:0099512,GO:0099568,GO:0099623,GO:0120025,GO:0120038,GO:1901016,GO:1901018,GO:1901019,GO:1901021,GO:1901360,GO:1901362,GO:1901379,GO:1901381,GO:1901564,GO:1901566,GO:1901576,GO:1903115,GO:1903169,GO:1903522,GO:1903779,GO:1904062,GO:1904064,GO:1904427,GO:1990778,GO:2001257,GO:2001259
Exons1
EggNOG free text desc.response to abiotic stimulus
EggNOG OGsCOG0666@1,KOG4177@2759
Cds size552
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681145263.174848-CDS-Ecto-sp8_S_contig9134:6443..69951681145263.174848-CDS-Ecto-sp8_S_contig9134:6443..6995Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig9134 6444..6995 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig9134.30456.1prot_Ecto-sp8_S_contig9134.30456.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig9134 6444..6995 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig9134.30456.1

>prot_Ecto-sp8_S_contig9134.30456.1 ID=prot_Ecto-sp8_S_contig9134.30456.1|Name=mRNA_Ecto-sp8_S_contig9134.30456.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=184bp
GADANRVDPAEKCGPLHVAAAGGHCSLVSDLLIGGAHPNARDLLRRTPLH
RAAYMGQDMVVAVLTDMPSTDKDILDKSELSPLMVASLRGHLSTVKTLLN
AGADVSIRTSHGNSALHWAARQGQVEVITAILEHGADVNASNYSGYTALH
AGCATNQTGAMDVLLDAGADMDRKYYSSFSPLYM
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mRNA from alignment at Ecto-sp8_S_contig9134:6444..6997+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig9134.30456.1 ID=mRNA_Ecto-sp8_S_contig9134.30456.1|Name=mRNA_Ecto-sp8_S_contig9134.30456.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=554bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9134:6444..6997+ (Ectocarpus species8 EcLAC_412)
GGAGCTGACGCAAACCGTGTTGACCCTGCTGAAAAATGCGGTCCTCTCCA
CGTCGCCGCAGCTGGAGGGCATTGTAGTCTAGTGAGCGATTTGCTGATAG
GCGGAGCTCATCCGAATGCGCGCGACTTGTTGAGGCGTACTCCTTTGCAC
CGTGCTGCTTACATGGGCCAAGACATGGTGGTGGCAGTGCTCACCGACAT
GCCGAGTACGGACAAGGACATTTTGGACAAGAGCGAGTTATCTCCGTTGA
TGGTGGCGTCGCTGAGGGGCCACCTCTCCACCGTCAAGACTCTTTTGAAC
GCCGGTGCTGATGTCAGCATACGTACCAGTCACGGAAATTCCGCTCTTCA
TTGGGCTGCCCGACAGGGACAGGTGGAGGTCATAACAGCCATCCTGGAGC
ACGGGGCCGACGTCAACGCTTCAAACTACTCTGGCTATACCGCTTTACAT
GCCGGCTGCGCGACGAATCAAACAGGCGCCATGGACGTGCTTCTCGACGC
CGGGGCCGACATGGATAGGAAATATTATAGTTCATTTTCACCTCTCTATA
TGGC
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig9134:6444..6997+

>mRNA_Ecto-sp8_S_contig9134.30456.1 ID=mRNA_Ecto-sp8_S_contig9134.30456.1|Name=mRNA_Ecto-sp8_S_contig9134.30456.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=552bp|location=Sequence derived from alignment at Ecto-sp8_S_contig9134:6444..6997+ (Ectocarpus species8 EcLAC_412)
GGAGCTGACGCAAACCGTGTTGACCCTGCTGAAAAATGCGGTCCTCTCCA
CGTCGCCGCAGCTGGAGGGCATTGTAGTCTAGTGAGCGATTTGCTGATAG
GCGGAGCTCATCCGAATGCGCGCGACTTGTTGAGGCGTACTCCTTTGCAC
CGTGCTGCTTACATGGGCCAAGACATGGTGGTGGCAGTGCTCACCGACAT
GCCGAGTACGGACAAGGACATTTTGGACAAGAGCGAGTTATCTCCGTTGA
TGGTGGCGTCGCTGAGGGGCCACCTCTCCACCGTCAAGACTCTTTTGAAC
GCCGGTGCTGATGTCAGCATACGTACCAGTCACGGAAATTCCGCTCTTCA
TTGGGCTGCCCGACAGGGACAGGTGGAGGTCATAACAGCCATCCTGGAGC
ACGGGGCCGACGTCAACGCTTCAAACTACTCTGGCTATACCGCTTTACAT
GCCGGCTGCGCGACGAATCAAACAGGCGCCATGGACGTGCTTCTCGACGC
CGGGGCCGACATGGATAGGAAATATTATAGTTCATTTTCACCTCTCTATA
TG
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