mRNA_Ecto-sp8_S_contig106727.1043.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_Ecto-sp8_S_contig106727.1043.1
Unique Name	mRNA_Ecto-sp8_S_contig106727.1043.1
Type	mRNA
Organism	Ectocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)

Homology

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A6EYB7_9GAMM (D-amino-acid dehydrogenase n=9 Tax=Gammaproteobacteria TaxID=1236 RepID=A6EYB7_9GAMM)

HSP 1 Score: 316 bits (809), Expect = 3.220e-105
Identity = 156/166 (93.98%), Postives = 162/166 (97.59%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            AVTP+EM AIEPTLAG+YYGGF+TESDSTGDIHKFTNGLA+AILRLGVKT YGHSVTELSADGSHAWVTSHDGE+QTRDTFDGVVICAGVGSR LAAKLGDRVNIYPVKGYSITVELDDQASQ+AAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSG NRDIRDD
Sbjct:  165 AVTPEEMRAIEPTLAGTYYGGFYTESDSTGDIHKFTNGLAEAILRLGVKTCYGHSVTELSADGSHAWVTSHDGEEQTRDTFDGVVICAGVGSRELAAKLGDRVNIYPVKGYSITVELDDQASQEAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGYNRDIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A0A7G2RW01_9GAMM (D-amino acid dehydrogenase n=7 Tax=Marinobacter TaxID=2742 RepID=A0A7G2RW01_9GAMM)

HSP 1 Score: 305 bits (781), Expect = 5.600e-101
Identity = 152/166 (91.57%), Postives = 159/166 (95.78%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            AVTP+EM +IEPTLAG+YYGGF+TESDSTGDIHKFTNGLA+AILRLGVKT Y HSVTELSAD SHAWVTS DGE+QTRDTFDGVVICAGVGSR LAAKLGDRVNIYPVKGYSITVELDDQASQ+AAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSG NRDIRDD
Sbjct:  165 AVTPEEMRSIEPTLAGTYYGGFYTESDSTGDIHKFTNGLAEAILRLGVKTCYEHSVTELSADQSHAWVTSFDGEEQTRDTFDGVVICAGVGSRELAAKLGDRVNIYPVKGYSITVELDDQASQEAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGYNRDIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A0A1E7PZF1_9GAMM (D-amino acid dehydrogenase n=7 Tax=root TaxID=1 RepID=A0A1E7PZF1_9GAMM)

HSP 1 Score: 300 bits (768), Expect = 5.200e-99
Identity = 148/166 (89.16%), Postives = 159/166 (95.78%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            AVTPDEM AIEPTLAGSYYGGF+TESDSTGDIHKFTNGLA+AILRLGVKT YGH+VTEL+AD SHAWVTS DGEQQTR+T+DGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVEL+D+ASQ AAPTVSLLDDATK+VTSRLGD RFRVAGTAEF+G NR+IRDD
Sbjct:  165 AVTPDEMRAIEPTLAGSYYGGFYTESDSTGDIHKFTNGLAEAILRLGVKTCYGHTVTELNADQSHAWVTSFDGEQQTRETYDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELEDEASQQAAPTVSLLDDATKLVTSRLGDTRFRVAGTAEFNGYNRNIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: R8B0C9_9GAMM (D-amino acid dehydrogenase small subunit n=3 Tax=Marinobacter TaxID=2742 RepID=R8B0C9_9GAMM)

HSP 1 Score: 298 bits (762), Expect = 3.610e-98
Identity = 147/166 (88.55%), Postives = 157/166 (94.58%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            AVTPDEM AIEPTLAG+YYGGF+TESDSTGDIHKFTNGLADAILRLGV+T YGH+VTELSAD +HAWVT+ DGEQQ RDTFDGVVICAGVGSR+LAAKLGDRVNIYPVKGYSITVELDD+ S+ +APTVSLLDDATKIVTSRLGD RFRVAGTAEFSG NRDIRDD
Sbjct:  165 AVTPDEMRAIEPTLAGTYYGGFYTESDSTGDIHKFTNGLADAILRLGVRTCYGHTVTELSADQNHAWVTADDGEQQNRDTFDGVVICAGVGSRSLAAKLGDRVNIYPVKGYSITVELDDEDSRKSAPTVSLLDDATKIVTSRLGDGRFRVAGTAEFSGYNRDIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: UPI0019D492A1 (D-amino acid dehydrogenase n=1 Tax=Marinobacter sp. LPB0319 TaxID=2769486 RepID=UPI0019D492A1)

HSP 1 Score: 296 bits (758), Expect = 1.250e-97
Identity = 145/165 (87.88%), Postives = 154/165 (93.33%), Query Frame = 1

Query:    4 VTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            VTPDEM AIEPTLAG+YYGGF+TESDSTGDIHKFTNGLA+AI RLGVKT YGHSV E+SAD  HAW+T+HDG+ QTRDTFDGVVICAGVGSR+LAAKLGDRVNIYPVKGYSITVELDD+ASQ AAPTVSLLDD TKIVTSRLGD RFRVAGTAEFSG NRDIRDD
Sbjct:  166 VTPDEMRAIEPTLAGTYYGGFYTESDSTGDIHKFTNGLAEAIKRLGVKTNYGHSVIEVSADDQHAWITAHDGDDQTRDTFDGVVICAGVGSRSLAAKLGDRVNIYPVKGYSITVELDDEASQKAAPTVSLLDDETKIVTSRLGDGRFRVAGTAEFSGYNRDIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A0A0K1UEV6_9GAMM (Amino acid dehydrogenase n=33 Tax=Marinobacter TaxID=2742 RepID=A0A0K1UEV6_9GAMM)

HSP 1 Score: 292 bits (748), Expect = 4.340e-96
Identity = 144/166 (86.75%), Postives = 155/166 (93.37%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            +VTP+EM +IEPTLAG+YYGGFFTESDSTGDIHKFTNGLADAI RLGVKT YGH+VTELSAD  +AWVT+HDG +Q+RDTFDGVVICAGVGSR LA KLGDRVNIYPVKGYSITVELDD+ASQ AAPTVSLLDDATKIVTSRLGD RFRVAGTAEFSG NRDI+DD
Sbjct:  165 SVTPEEMKSIEPTLAGNYYGGFFTESDSTGDIHKFTNGLADAIKRLGVKTCYGHTVTELSADERNAWVTAHDGTEQSRDTFDGVVICAGVGSRGLAKKLGDRVNIYPVKGYSITVELDDEASQKAAPTVSLLDDATKIVTSRLGDGRFRVAGTAEFSGYNRDIKDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A0A0P7Z533_9GAMM (Glycine oxidase (Deaminating) n=1 Tax=Marinobacter sp. HL-58 TaxID=1479237 RepID=A0A0P7Z533_9GAMM)

HSP 1 Score: 292 bits (748), Expect = 6.830e-96
Identity = 146/165 (88.48%), Postives = 154/165 (93.33%), Query Frame = 1

Query:    4 VTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            VTP EM  IEPTLAG+YYGGFFT+SDSTGDIHKFTNGLADAI RLGVKT YGHSVTELSAD SHAWVTS +  +QTRDTFDGVVICAGVGSR LAAKLGDRVNIYPVKGYSITV+LDDQAS+++APTVSLLDDATKIVTSRLGDDRFRVAGTAEFSG NRDIRDD
Sbjct:  173 VTPAEMRDIEPTLAGTYYGGFFTDSDSTGDIHKFTNGLADAIERLGVKTCYGHSVTELSADQSHAWVTSEEDGEQTRDTFDGVVICAGVGSRELAAKLGDRVNIYPVKGYSITVQLDDQASRESAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGFNRDIRDD 337

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: UPI001F015C16 (D-amino acid dehydrogenase n=1 Tax=Marinobacter similis TaxID=1420916 RepID=UPI001F015C16)

HSP 1 Score: 291 bits (746), Expect = 1.590e-95
Identity = 143/165 (86.67%), Postives = 156/165 (94.55%), Query Frame = 1

Query:    4 VTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            VTP+EM +IEPTLAG+YYGGF+TESDSTGDIHKFTNGLADAI R GVKT YGH+VTELSAD S AWVTS DG++QTR+T+DGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDD+ASQ AAPTVSLLDDATK+VTSRLGDDRFRVAGTAEF+G NR+IRDD
Sbjct:  183 VTPEEMKSIEPTLAGTYYGGFYTESDSTGDIHKFTNGLADAIQRRGVKTNYGHTVTELSADASSAWVTSFDGKEQTRETYDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDEASQQAAPTVSLLDDATKLVTSRLGDDRFRVAGTAEFNGYNRNIRDD 347

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: A0A0H4I2E3_9GAMM (Amino acid dehydrogenase n=19 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A0H4I2E3_9GAMM)

HSP 1 Score: 290 bits (742), Expect = 3.730e-95
Identity = 142/166 (85.54%), Postives = 156/166 (93.98%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIRDD 498
            AVTP+EM AIEPTLAG++YGGFFTESDSTGDIHKFT+GLADAI R GVKT YGH+VTELSAD SHAW+TS DGEQQ R+TFDGVV+CAGVGSR LAAKLGDRVNIYPVKGYSITVELDD+AS+ AAPTVSLLDDATK+VTSRLGDDRFR+AGTAEF+G NR+IRDD
Sbjct:  165 AVTPNEMKAIEPTLAGNFYGGFFTESDSTGDIHKFTSGLADAIQRRGVKTCYGHTVTELSADQSHAWITSFDGEQQIRNTFDGVVVCAGVGSRELAAKLGDRVNIYPVKGYSITVELDDEASRRAAPTVSLLDDATKLVTSRLGDDRFRIAGTAEFNGYNRNIRDD 330

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BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Match: UPI0011E62155 (D-amino acid dehydrogenase n=1 Tax=Marinobacter fonticola TaxID=2603215 RepID=UPI0011E62155)

HSP 1 Score: 270 bits (690), Expect = 3.230e-87
Identity = 134/164 (81.71%), Postives = 145/164 (88.41%), Query Frame = 1

Query:    1 AVTPDEMLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSVTELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIYPVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEFSGSNRDIR 492
            AVTP+EM AIEPTLAG+YYGGFFTESDSTGDIHKFT GLA A  RLGV+T YGH+VT+L AD S AWVTS DGE + R  FDG+V+CAGVGSRALAAKLGDRVNIYPVKGYSITV LD+  SQ++APTVSLLDD TKIVTSRLGDDRFRVAGTAEFSG NRDIR
Sbjct:  165 AVTPEEMRAIEPTLAGTYYGGFFTESDSTGDIHKFTQGLAAATERLGVETRYGHAVTDLGADQSRAWVTSSDGEDEQRHEFDGLVVCAGVGSRALAAKLGDRVNIYPVKGYSITVHLDEPQSQESAPTVSLLDDETKIVTSRLGDDRFRVAGTAEFSGYNRDIR 328

The following BLAST results are available for this feature:

BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot

BLAST of mRNA_Ecto-sp8_S_contig106727.1043.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A6EYB7_9GAMM	3.220e-105	93.98	D-amino-acid dehydrogenase n=9 Tax=Gammaproteobact...	[more]
A0A7G2RW01_9GAMM	5.600e-101	91.57	D-amino acid dehydrogenase n=7 Tax=Marinobacter Ta...	[more]
A0A1E7PZF1_9GAMM	5.200e-99	89.16	D-amino acid dehydrogenase n=7 Tax=root TaxID=1 Re...	[more]
R8B0C9_9GAMM	3.610e-98	88.55	D-amino acid dehydrogenase small subunit n=3 Tax=M...	[more]
UPI0019D492A1	1.250e-97	87.88	D-amino acid dehydrogenase n=1 Tax=Marinobacter sp...	[more]
A0A0K1UEV6_9GAMM	4.340e-96	86.75	Amino acid dehydrogenase n=33 Tax=Marinobacter Tax...	[more]
A0A0P7Z533_9GAMM	6.830e-96	88.48	Glycine oxidase (Deaminating) n=1 Tax=Marinobacter...	[more]
UPI001F015C16	1.590e-95	86.67	D-amino acid dehydrogenase n=1 Tax=Marinobacter si...	[more]
A0A0H4I2E3_9GAMM	3.730e-95	85.54	Amino acid dehydrogenase n=19 Tax=Gammaproteobacte...	[more]
UPI0011E62155	3.230e-87	81.71	D-amino acid dehydrogenase n=1 Tax=Marinobacter fo...	[more]

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Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Ecto-sp8_S_contig106727	contig	Ecto-sp8_S_contig106727:3..500 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
Ectocarpus species8 EcLAC_412 OGS1.0	2022-07-08

Properties


Property Name	Value
Taxonomic scope	Bacteria
Stop	0
Start	1
Seed ortholog score	303.5
Seed ortholog evalue	5.7e-80
Seed eggNOG ortholog	443152.MDG893_18322
Preferred name	dadA
Model size	498
KEGG rclass	RC00006,RC00025
KEGG ko	ko:K00285
KEGG Reaction	R01374,R09493
KEGG Pathway	ko00360,map00360
Hectar predicted targeting category	other localisation
Exons	1
EggNOG free text desc.	COG0665 Glycine D-amino acid oxidases (deaminating)
EggNOG OGs	1MVIZ@1224,1RQ50@1236,46524@72275,COG0665@1,COG0665@2
EC	1.4.5.1
Cds size	480
COG Functional cat.	C
Best tax level	Alteromonadaceae
Best eggNOG OG	NA\|NA\|NA
BRITE	ko00000,ko00001,ko01000

Relationships

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681143163.5212228-UTR-Ecto-sp8_S_contig106727:2..20	1681143163.5212228-UTR-Ecto-sp8_S_contig106727:2..20	Ectocarpus species8 EcLAC_412	UTR	Ecto-sp8_S_contig106727 3..20 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681143163.5364785-CDS-Ecto-sp8_S_contig106727:20..500	1681143163.5364785-CDS-Ecto-sp8_S_contig106727:20..500	Ectocarpus species8 EcLAC_412	CDS	Ecto-sp8_S_contig106727 21..500 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_Ecto-sp8_S_contig106727.1043.1	prot_Ecto-sp8_S_contig106727.1043.1	Ectocarpus species8 EcLAC_412	polypeptide	Ecto-sp8_S_contig106727 21..500 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at Ecto-sp8_S_contig106727:3..500+
coding sequence from alignment at Ecto-sp8_S_contig106727:3..500+

protein sequence of mRNA_Ecto-sp8_S_contig106727.1043.1

>prot_Ecto-sp8_S_contig106727.1043.1 ID=prot_Ecto-sp8_S_contig106727.1043.1|Name=mRNA_Ecto-sp8_S_contig106727.1043.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=160bp
MLAIEPTLAGSYYGGFFTESDSTGDIHKFTNGLADAILRLGVKTYYGHSV
TELSADGSHAWVTSHDGEQQTRDTFDGVVICAGVGSRALAAKLGDRVNIY
PVKGYSITVELDDQASQDAAPTVSLLDDATKIVTSRLGDDRFRVAGTAEF
SGSNRDIRDD

mRNA from alignment at Ecto-sp8_S_contig106727:3..500+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_Ecto-sp8_S_contig106727.1043.1 ID=mRNA_Ecto-sp8_S_contig106727.1043.1|Name=mRNA_Ecto-sp8_S_contig106727.1043.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=498bp|location=Sequence derived from alignment at Ecto-sp8_S_contig106727:3..500+ (Ectocarpus species8 EcLAC_412)
GCCGTTACCCCAGATGAAATGCTCGCTATCGAACCTACGCTGGCAGGGAG
TTACTACGGTGGTTTCTTCACCGAGAGTGATTCCACTGGTGACATCCACA
AGTTCACCAACGGCCTGGCAGATGCGATCCTGCGCCTGGGGGTAAAAACC
TACTATGGACACTCGGTGACCGAGCTGAGTGCAGACGGGTCCCATGCCTG
GGTAACCTCCCATGATGGCGAGCAGCAGACTCGTGACACCTTTGACGGGG
TAGTGATCTGTGCCGGTGTCGGCAGCCGGGCTTTGGCTGCCAAGCTGGGT
GATCGCGTCAACATATACCCGGTGAAGGGGTATTCCATCACCGTTGAGCT
AGACGACCAGGCTTCTCAAGACGCGGCACCGACCGTCAGTCTGCTGGACG
ACGCCACCAAGATTGTCACAAGCCGGCTGGGAGATGATCGTTTCCGGGTT
GCGGGTACGGCGGAATTCAGCGGCTCTAACCGTGATATCAGGGATGAC

Coding sequence (CDS) from alignment at Ecto-sp8_S_contig106727:3..500+

>mRNA_Ecto-sp8_S_contig106727.1043.1 ID=mRNA_Ecto-sp8_S_contig106727.1043.1|Name=mRNA_Ecto-sp8_S_contig106727.1043.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=480bp|location=Sequence derived from alignment at Ecto-sp8_S_contig106727:3..500+ (Ectocarpus species8 EcLAC_412)
ATGCTCGCTATCGAACCTACGCTGGCAGGGAGTTACTACGGTGGTTTCTT
CACCGAGAGTGATTCCACTGGTGACATCCACAAGTTCACCAACGGCCTGG
CAGATGCGATCCTGCGCCTGGGGGTAAAAACCTACTATGGACACTCGGTG
ACCGAGCTGAGTGCAGACGGGTCCCATGCCTGGGTAACCTCCCATGATGG
CGAGCAGCAGACTCGTGACACCTTTGACGGGGTAGTGATCTGTGCCGGTG
TCGGCAGCCGGGCTTTGGCTGCCAAGCTGGGTGATCGCGTCAACATATAC
CCGGTGAAGGGGTATTCCATCACCGTTGAGCTAGACGACCAGGCTTCTCA
AGACGCGGCACCGACCGTCAGTCTGCTGGACGACGCCACCAAGATTGTCA
CAAGCCGGCTGGGAGATGATCGTTTCCGGGTTGCGGGTACGGCGGAATTC
AGCGGCTCTAACCGTGATATCAGGGATGAC