mRNA_Ecto-sp8_S_contig105807.913.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig105807.913.1
Unique NamemRNA_Ecto-sp8_S_contig105807.913.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A6A4VHN1_AMPAM (Eukaryotic peptide chain release factor subunit 1 n=3 Tax=Thoracicalcarea TaxID=2899756 RepID=A0A6A4VHN1_AMPAM)

HSP 1 Score: 288 bits (738), Expect = 3.040e-97
Identity = 139/170 (81.76%), Postives = 156/170 (91.76%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIELSAECL+NVKFIQEKKLIG+YFDEISQDT KYCFGV+DTL ALE+GAVETLICWENLDIQR+ LKNH SGEEK L L   +EKD++HF D ++GVEMEL +++PLLEWLANNYK FGA+LEIITDRSQEGSQ+VRGFGG+GGMLRYTV+FQ
Sbjct:   28 IKLVDVSYGGENGFNQAIELSAECLANVKFIQEKKLIGRYFDEISQDTGKYCFGVKDTLAALELGAVETLICWENLDIQRIVLKNHASGEEKILHLTPEEEKDRSHFTDSDSGVEMELVDSMPLLEWLANNYKSFGASLEIITDRSQEGSQFVRGFGGVGGMLRYTVNFQ 197
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: C1BTM2_LEPSM (Eukaryotic peptide chain release factor subunit 1 n=3 Tax=Neocopepoda TaxID=116569 RepID=C1BTM2_LEPSM)

HSP 1 Score: 292 bits (748), Expect = 1.260e-95
Identity = 143/170 (84.12%), Postives = 154/170 (90.59%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L+NVKFIQEKKLIG+YFD+ISQDT KYCFGVEDTLKALEMGAVETLICWENLDIQR  LKNHG+GEEK L L S QEKD THF D+ETGVE+ELE+N P LEWLANNYK +GA LEIITD+SQEGSQ+VRGFGGIGGMLRY VDFQ
Sbjct:  250 IKLVDVSYGGENGFNQAIELAAESLANVKFIQEKKLIGRYFDQISQDTGKYCFGVEDTLKALEMGAVETLICWENLDIQRYVLKNHGAGEEKVLHLTSEQEKDMTHFTDKETGVELELEDNTPFLEWLANNYKSYGATLEIITDKSQEGSQFVRGFGGIGGMLRYKVDFQ 419
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A553NXS9_TIGCA (Eukaryotic peptide chain release factor subunit 1 n=1 Tax=Tigriopus californicus TaxID=6832 RepID=A0A553NXS9_TIGCA)

HSP 1 Score: 291 bits (744), Expect = 5.360e-95
Identity = 143/170 (84.12%), Postives = 154/170 (90.59%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L+NVKFIQEKKLIGKYFD+ISQ+T KYCFGVEDTL+ LEMGAVETLICWENLDIQR TLKNH +GEEK L L+S QEKD THF D++TGVE+ELEENIP LEWLANNYK FGA LEIITDRSQEGSQ+VRGFGGIGGMLRY VD Q
Sbjct:  252 IKLVDVSYGGENGFNQAIELAAESLANVKFIQEKKLIGKYFDQISQETGKYCFGVEDTLRCLEMGAVETLICWENLDIQRFTLKNHSTGEEKILHLSSEQEKDMTHFTDKDTGVELELEENIPFLEWLANNYKNFGATLEIITDRSQEGSQFVRGFGGIGGMLRYKVDLQ 421
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A8D9ENT7_9HEMI (Eukaryotic peptide chain release factor subunit 1 n=2 Tax=Cacopsylla melanoneura TaxID=428564 RepID=A0A8D9ENT7_9HEMI)

HSP 1 Score: 277 bits (709), Expect = 5.090e-93
Identity = 136/167 (81.44%), Postives = 149/167 (89.22%), Query Frame = 1 
Query:   10 VDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIGKYFDEISQDT KYCFGV+DTL+ALE+G+VETLICWENLDIQR  LKNH +GEE+ L L   QEKDK+HF D+ETGVE+EL E  PLLEWLANNYK FGA LEIITD+SQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:   17 VDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGKYFDEISQDTGKYCFGVDDTLRALELGSVETLICWENLDIQRYVLKNHSTGEERVLHLTPEQEKDKSHFTDKETGVELELVECQPLLEWLANNYKSFGATLEIITDKSQEGSQFVRGFGGIGGILRYKVDFQ 183
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A1D2MEI3_ORCCI (Eukaryotic peptide chain release factor subunit 1 n=4 Tax=Hexapoda TaxID=6960 RepID=A0A1D2MEI3_ORCCI)

HSP 1 Score: 286 bits (731), Expect = 5.540e-93
Identity = 141/170 (82.94%), Postives = 151/170 (88.82%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YFDEISQDT KYCFGVEDTLKALE+GAVETLICWENLDIQR  LKNH +GEE+ L L + QEKDKTHF D+ETGVE+EL E  P LEW+ANNYK FGA LEIITDRSQEGSQ+VRGFGGIGGMLRY VDFQ
Sbjct:  257 IKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALELGAVETLICWENLDIQRYVLKNHATGEERVLHLTAEQEKDKTHFTDKETGVELELVECTPFLEWIANNYKTFGATLEIITDRSQEGSQFVRGFGGIGGMLRYKVDFQ 426
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A7F5RC64_AGRPL (eukaryotic peptide chain release factor subunit 1-like n=1 Tax=Agrilus planipennis TaxID=224129 RepID=A0A7F5RC64_AGRPL)

HSP 1 Score: 276 bits (706), Expect = 1.980e-92
Identity = 138/170 (81.18%), Postives = 147/170 (86.47%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YFDEISQDT KYCFGVEDTLK LE+GAVETLICWENLDIQR  LKNH  G E  L L   QEKDK+HF D+ETGVE+EL E  PLLEWLANNYK FGA LEIITD+SQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:   21 IKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKGLELGAVETLICWENLDIQRYVLKNHTVGTETVLHLTPEQEKDKSHFTDKETGVELELVECQPLLEWLANNYKNFGATLEIITDKSQEGSQFVRGFGGIGGLLRYKVDFQ 190
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A1B6DYM6_9HEMI (Eukaryotic peptide chain release factor subunit 1 n=6 Tax=Neoptera TaxID=33340 RepID=A0A1B6DYM6_9HEMI)

HSP 1 Score: 280 bits (717), Expect = 7.190e-91
Identity = 138/170 (81.18%), Postives = 151/170 (88.82%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YFDEISQDT KYCFGVEDTL+ALE+G+VETLICWENLDIQR  LKNH + EEK L L   QEKDKTHF D+E+GVE+EL E+ PLLEWLANNYK FGA LEIITD+SQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:  255 IKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHTNSEEKVLHLTPEQEKDKTHFTDKESGVELELVESQPLLEWLANNYKSFGATLEIITDKSQEGSQFVRGFGGIGGLLRYKVDFQ 424
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A7R9A2P6_9CRUS (Eukaryotic peptide chain release factor subunit 1 n=1 Tax=Darwinula stevensoni TaxID=69355 RepID=A0A7R9A2P6_9CRUS)

HSP 1 Score: 280 bits (716), Expect = 9.070e-91
Identity = 139/170 (81.76%), Postives = 151/170 (88.82%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VD+SYGGENGFNQAIEL++  L+NVKFIQEKKLIG+YFDEISQDT KYCFGVEDTLKALE+GAVETLICWENLDI R  LKNH +GEEK L LN  QEKDKTHF D+ETGVE+EL E + LLEWLANNYK FGA LEIITDRSQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:  253 IKIVDISYGGENGFNQAIELASGSLANVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLKALELGAVETLICWENLDITRFVLKNHQTGEEKVLHLNPEQEKDKTHFTDKETGVELELIECMALLEWLANNYKNFGATLEIITDRSQEGSQFVRGFGGIGGILRYKVDFQ 422
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A0A9Y8A5_LYGHE (Eukaryotic peptide chain release factor subunit 1 n=185 Tax=Hexapoda TaxID=6960 RepID=A0A0A9Y8A5_LYGHE)

HSP 1 Score: 280 bits (715), Expect = 1.400e-90
Identity = 137/170 (80.59%), Postives = 151/170 (88.82%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YFDEISQDT KYCFGVEDTL+ALE+G+VETLICWENLDIQR  LKNH + EEK L L   QEKDK+HF D+E+GVE+EL E+ PLLEWLANNYK FGA LEIITD+SQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:  255 IKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGKYCFGVEDTLRALELGSVETLICWENLDIQRYVLKNHSNAEEKVLHLTPEQEKDKSHFTDKESGVELELVESQPLLEWLANNYKSFGATLEIITDKSQEGSQFVRGFGGIGGLLRYKVDFQ 424
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BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Match: A0A1D2NDZ5_ORCCI (Eukaryotic peptide chain release factor subunit 1 n=2 Tax=Entomobryomorpha TaxID=730330 RepID=A0A1D2NDZ5_ORCCI)

HSP 1 Score: 280 bits (715), Expect = 1.530e-90
Identity = 137/170 (80.59%), Postives = 150/170 (88.24%), Query Frame = 1 
Query:    1 IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQKYCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQEKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGSQYVRGFGGIGGMLRYTVDFQ 510
            IK VDVSYGGENGFNQAIEL+AE L NVKFIQEKKLIG+YFDEISQDT +YCFGVEDTLKALE+GAVETLICWENLDIQR  LKNHG+ EEK L L   QEKDKTHF D+E+GVE+EL +  P LEW+ANNYK FGA LEIITD+SQEGSQ+VRGFGGIGG+LRY VDFQ
Sbjct:  259 IKLVDVSYGGENGFNQAIELAAESLQNVKFIQEKKLIGRYFDEISQDTGRYCFGVEDTLKALELGAVETLICWENLDIQRYVLKNHGNAEEKILHLTPDQEKDKTHFTDKESGVELELVDCTPFLEWIANNYKTFGATLEIITDKSQEGSQFVRGFGGIGGLLRYKVDFQ 428
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig105807.913.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6A4VHN1_AMPAM3.040e-9781.76Eukaryotic peptide chain release factor subunit 1 ... [more]
C1BTM2_LEPSM1.260e-9584.12Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A553NXS9_TIGCA5.360e-9584.12Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A8D9ENT7_9HEMI5.090e-9381.44Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A1D2MEI3_ORCCI5.540e-9382.94Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A7F5RC64_AGRPL1.980e-9281.18eukaryotic peptide chain release factor subunit 1-... [more]
A0A1B6DYM6_9HEMI7.190e-9181.18Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A7R9A2P6_9CRUS9.070e-9181.76Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A0A9Y8A5_LYGHE1.400e-9080.59Eukaryotic peptide chain release factor subunit 1 ... [more]
A0A1D2NDZ5_ORCCI1.530e-9080.59Eukaryotic peptide chain release factor subunit 1 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig105807contigEcto-sp8_S_contig105807:1..510 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeArthropoda
Stop0
Start0
Seed ortholog score283.9
Seed ortholog evalue5e-74
Seed eggNOG ortholog13249.RPRC006509-PA
Model size510
KEGG koko:K03265
KEGG Pathwayko03015,map03015
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003676,GO:0003723,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006412,GO:0006415,GO:0006518,GO:0006807,GO:0007154,GO:0007165,GO:0007166,GO:0007224,GO:0008079,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0016043,GO:0019538,GO:0022411,GO:0023052,GO:0032984,GO:0034641,GO:0034645,GO:0043043,GO:0043170,GO:0043603,GO:0043604,GO:0043624,GO:0043933,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0065007,GO:0071704,GO:0071840,GO:0097159,GO:1901363,GO:1901564,GO:1901566,GO:1901576
Exons1
EggNOG free text desc.eRF1_1
EggNOG OGs38E1W@33154,3BCAN@33208,3D1R5@33213,3EA07@33342,3SJCQ@50557,41X8R@6656,COG1503@1,KOG0688@2759
Cds size510
COG Functional cat.J
Best tax levelParaneoptera
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03012,ko03019
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143156.1121852-CDS-Ecto-sp8_S_contig105807:0..5101681143156.1121852-CDS-Ecto-sp8_S_contig105807:0..510Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig105807 1..510 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig105807.913.1prot_Ecto-sp8_S_contig105807.913.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig105807 1..510 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig105807.913.1

>prot_Ecto-sp8_S_contig105807.913.1 ID=prot_Ecto-sp8_S_contig105807.913.1|Name=mRNA_Ecto-sp8_S_contig105807.913.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=170bp
IKTVDVSYGGENGFNQAIELSAECLSNVKFIQEKKLIGKYFDEISQDTQK
YCFGVEDTLKALEMGAVETLICWENLDIQRVTLKNHGSGEEKTLFLNSSQ
EKDKTHFMDQETGVEMELEENIPLLEWLANNYKKFGAALEIITDRSQEGS
QYVRGFGGIGGMLRYTVDFQ
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mRNA from alignment at Ecto-sp8_S_contig105807:1..510+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig105807.913.1 ID=mRNA_Ecto-sp8_S_contig105807.913.1|Name=mRNA_Ecto-sp8_S_contig105807.913.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=510bp|location=Sequence derived from alignment at Ecto-sp8_S_contig105807:1..510+ (Ectocarpus species8 EcLAC_412)
ATCAAGACGGTGGATGTCAGCTACGGCGGGGAGAACGGCTTCAATCAGGC
CATTGAGCTGTCTGCAGAGTGCCTCTCCAATGTTAAGTTCATCCAGGAGA
AGAAGTTGATTGGGAAATACTTTGACGAGATCTCCCAGGATACGCAGAAG
TACTGCTTTGGTGTCGAAGACACCCTCAAGGCTTTGGAGATGGGGGCGGT
AGAGACTCTGATCTGCTGGGAAAATTTGGATATTCAGCGTGTTACTCTGA
AAAACCACGGATCTGGGGAAGAGAAGACCTTGTTCTTGAATTCGTCGCAG
GAGAAGGACAAGACCCACTTCATGGACCAAGAGACTGGAGTCGAGATGGA
GTTGGAGGAGAACATTCCTCTGCTGGAGTGGTTGGCCAACAACTACAAGA
AGTTTGGAGCTGCTCTAGAAATTATTACTGATAGGAGTCAAGAAGGTAGC
CAGTACGTTAGGGGGTTCGGTGGGATTGGGGGAATGCTACGATACACAGT
GGATTTTCAG
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig105807:1..510+

>mRNA_Ecto-sp8_S_contig105807.913.1 ID=mRNA_Ecto-sp8_S_contig105807.913.1|Name=mRNA_Ecto-sp8_S_contig105807.913.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=510bp|location=Sequence derived from alignment at Ecto-sp8_S_contig105807:1..510+ (Ectocarpus species8 EcLAC_412)
ATCAAGACGGTGGATGTCAGCTACGGCGGGGAGAACGGCTTCAATCAGGC
CATTGAGCTGTCTGCAGAGTGCCTCTCCAATGTTAAGTTCATCCAGGAGA
AGAAGTTGATTGGGAAATACTTTGACGAGATCTCCCAGGATACGCAGAAG
TACTGCTTTGGTGTCGAAGACACCCTCAAGGCTTTGGAGATGGGGGCGGT
AGAGACTCTGATCTGCTGGGAAAATTTGGATATTCAGCGTGTTACTCTGA
AAAACCACGGATCTGGGGAAGAGAAGACCTTGTTCTTGAATTCGTCGCAG
GAGAAGGACAAGACCCACTTCATGGACCAAGAGACTGGAGTCGAGATGGA
GTTGGAGGAGAACATTCCTCTGCTGGAGTGGTTGGCCAACAACTACAAGA
AGTTTGGAGCTGCTCTAGAAATTATTACTGATAGGAGTCAAGAAGGTAGC
CAGTACGTTAGGGGGTTCGGTGGGATTGGGGGAATGCTACGATACACAGT
GGATTTTCAG
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