mRNA_Ecto-sp8_S_contig103445.559.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig103445.559.1
Unique NamemRNA_Ecto-sp8_S_contig103445.559.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A1B6DZ47_9HEMI (Myosin_tail_1 domain-containing protein (Fragment) n=1 Tax=Clastoptera arizonana TaxID=38151 RepID=A0A1B6DZ47_9HEMI)

HSP 1 Score: 153 bits (387), Expect = 3.910e-44
Identity = 82/161 (50.93%), Postives = 116/161 (72.05%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGA 483
            +TQLEDTK LAD E+++RA LL KF+ +  DL+NLRE+++ EA  K+D  + LSKA AE QLW+++YE+EG+ R EELE A+ KLQA++ E EE ++ L  KV   EKTK RL ++++D+ +E +R  A A   EK+AK  DK++GEW  K  D+AAE+ A
Sbjct:   32 TTQLEDTKKLADEESRERATLLGKFRNLEHDLDNLREQVDEEAEGKADIQRQLSKANAEAQLWRTKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKVIALEKTKQRLSTEVEDLQLEVDRATAIANAAEKKAKAIDKIIGEWKLKVDDLAAELDA 192
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BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: B0W187_CULQU (Myosin-2 heavy chain n=7 Tax=Culex TaxID=53527 RepID=B0W187_CULQU)

HSP 1 Score: 164 bits (415), Expect = 1.110e-43
Identity = 90/174 (51.72%), Postives = 121/174 (69.54%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            S QLEDTK LAD E+++RA LL KF+ +  DL+NLR+++E EA  K D  + LSK  AE QLW+SRYETEG+ R EELE  + KLQA++ E EE ++ L  KV   EKTK RL ++++D+ +E  R    A   EK+ ++FDK++GEW  K  D+A E+ ASQ E RNYS+ELF
Sbjct: 1316 SQQLEDTKRLADEESRERATLLGKFRNLEHDLDNLRDQVEEEAEGKGDIRRQLSKTYAEAQLWRSRYETEGVSRAEELEEGKRKLQARLAEAEETIESLTQKVIALEKTKQRLSTEIEDLQLEVGRSTVIANSAEKKHRSFDKIIGEWKLKVDDLAGELDASQKECRNYSTELF 1489
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BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A8D8AKM5_CULPI (Myosin heavy chain, muscle (Fragment) n=4 Tax=Culex pipiens TaxID=7175 RepID=A0A8D8AKM5_CULPI)

HSP 1 Score: 162 bits (411), Expect = 2.830e-43
Identity = 90/174 (51.72%), Postives = 120/174 (68.97%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            S QLEDTK LAD E+++RA LL KF+ +  DL+NLR+++E EA  K D  + LSK  AE QLW+SRYETEG+ R EELE  + KLQA++ E EE ++ L  KV   EKTK RL ++++D+ +E  R    A   EK+ + FDK++GEW  K  D+A E+ ASQ E RNYS+ELF
Sbjct:  387 SQQLEDTKRLADEESRERATLLGKFRNLEHDLDNLRDQVEEEAEGKGDIRRQLSKTYAEAQLWRSRYETEGVSRAEELEEGKRKLQARLAEAEETIESLTQKVIALEKTKQRLSTEIEDLQLEVGRSTVIANSAEKKHRLFDKIIGEWKLKVDDLAGELDASQKECRNYSTELF 560
[Close]
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: UPI001954DA61 (uncharacterized protein LOC120424957 n=1 Tax=Culex pipiens pallens TaxID=42434 RepID=UPI001954DA61)

HSP 1 Score: 162 bits (411), Expect = 3.960e-43
Identity = 89/174 (51.15%), Postives = 121/174 (69.54%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            S QLEDTK LAD E+++RA LL KF+ +  DL+NLR+++E EA  K D  + LSK  AE QLW+SRYE+EG+ R EELE  + KLQA++ E EE ++ L  KV   EKTK RL ++++D+ +E  R    A   EK+ ++FDK++GEW  K  D+A E+ ASQ E RNYS+ELF
Sbjct: 3286 SQQLEDTKRLADEESRERATLLGKFRNLEHDLDNLRDQVEEEAEGKGDIRRQLSKTYAEAQLWRSRYESEGVSRAEELEEGKRKLQARLAEAEETIESLTQKVIALEKTKQRLSTEIEDLQLEVGRSTVIANSAEKKHRSFDKIIGEWKLKVDDLAGELDASQKECRNYSTELF 3459
[Close]
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: UPI000C7659AC (myosin heavy chain, muscle-like n=1 Tax=Eurytemora affinis TaxID=88015 RepID=UPI000C7659AC)

HSP 1 Score: 162 bits (409), Expect = 7.140e-43
Identity = 82/141 (58.16%), Postives = 113/141 (80.14%), Query Frame = 1 
Query:   67 TKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQ 489
            TKFK +ST++ENLR ++E EA RK+D L+ALSKAQAEIQLW+S++ETE +GRI+ELE ++AKL  ++ E EE +D L TKV+     K R+ ++L+++ MEYER HAAA+ITEKR +NFDKV+GEW +KA D+ AE+ AS+
Sbjct: 1329 TKFKGLSTEVENLRMRIEEEAERKNDVLRALSKAQAEIQLWRSKFETEALGRIDELESSKAKLNVRVQEAEETIDSLNTKVSATXXXKHRIEAELEEMQMEYERTHAAAVITEKRGRNFDKVVGEWKAKADDLLAELEASR 1469
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BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A553PK51_TIGCA (Uncharacterized protein n=1 Tax=Tigriopus californicus TaxID=6832 RepID=A0A553PK51_TIGCA)

HSP 1 Score: 156 bits (395), Expect = 5.440e-41
Identity = 86/174 (49.43%), Postives = 118/174 (67.82%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            +TQLED+K LA+AE ++R  LL K K +  DLE +R++LE E   K +  + LSKA A+I LWK+RYET+G+ R EE+E  + K+ A++ E EE +  LQ K+   EK+K RL  +L+DIS +YER +  A I EKR +NFDKV+ EW  K  D+  E+ ASQ E RN+SSE F
Sbjct: 1276 TTQLEDSKRLAEAETRERINLLGKMKNLEHDLEIMRDQLEEEYEAKQEIERQLSKALADINLWKTRYETDGLARTEEIERDKGKVAARLAEAEETISALQEKIGVLEKSKVRLTGELEDISTDYERFNTNAAILEKRGRNFDKVITEWKCKGDDLQGEIEASQSECRNFSSEFF 1449
[Close]
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A226DJW9_FOLCA (Myosin heavy chain, muscle n=1 Tax=Folsomia candida TaxID=158441 RepID=A0A226DJW9_FOLCA)

HSP 1 Score: 151 bits (382), Expect = 3.050e-39
Identity = 82/167 (49.10%), Postives = 116/167 (69.46%), Query Frame = 1 
Query:   22 KALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            K +AD E+++RA LL KF+ +  D + LRE+L+ EA  K+D ++ LSKA AE QLW+++YE+EG+ R EELE  + KLQA++ E EE ++ L  KV   EK+K RL ++L+D+ +E ER  + A   EKRAK FDK + EW  +  D+AAE+  SQ E RNYS+ELF
Sbjct: 1320 KRVADEESRERAVLLAKFRTVEHDQDGLREQLDGEAEGKADLVRQLSKANAEAQLWRAKYESEGVARAEELEETKRKLQARLTEAEETIESLNQKVIALEKSKQRLATELEDMQLEVERAKSLANAMEKRAKAFDKTVSEWKQRVDDLAAELDNSQKECRNYSTELF 1486
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BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A0K2T980_LEPSM (Myosin heavy chain 1 [Tribolium castaneum] n=3 Tax=Lepeophtheirus salmonis TaxID=72036 RepID=A0A0K2T980_LEPSM)

HSP 1 Score: 149 bits (375), Expect = 2.660e-38
Identity = 80/174 (45.98%), Postives = 121/174 (69.54%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            +TQLED K LADAE ++R  LL K + +  +L  ++E L+ E   K +  + LSKA A++QLWK+R+ETEG+ RIEE+E  R+K+ +++ E E+ +  LQ K+A  EK+K R+ ++ +D++ E +R +  A I EKR +NFDKV+ EW  KA D+++E+ ASQ E RN+SSE F
Sbjct: 1339 TTQLEDAKRLADAETRERINLLGKMRNLEHELIIIKEHLDQEFDSKQEVERQLSKAFADVQLWKTRFETEGVARIEEIERDRSKISSRLREAEDTISALQEKIAVLEKSKSRMSTESEDLTSECDRQNTNASIIEKRGRNFDKVINEWKCKAEDLSSEITASQSECRNFSSEYF 1512
[Close]
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: A0A310SIQ5_9HYME (Myosin heavy chain, muscle n=1 Tax=Eufriesea mexicana TaxID=516756 RepID=A0A310SIQ5_9HYME)

HSP 1 Score: 148 bits (374), Expect = 3.500e-38
Identity = 80/162 (49.38%), Postives = 114/162 (70.37%), Query Frame = 1 
Query:    1 STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDALKALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGAS 486
            +TQLEDTK LAD E+++RA LL KF+ +  DL+N+RE++      K+D  + LSKA AE QLW+++YE+EG+ R EELE A+ KLQA++ E EE ++ L  KV   EKTK RL ++++D+ +E +R  A A   EK+ K FDK++GEW  K  D+AAE+ AS
Sbjct:  548 TTQLEDTKRLADEESRERATLLGKFRNLEHDLDNIREQVXXXXEGKADLQRQLSKANAEAQLWRTKYESEGVARAEELEEAKRKLQARLAEAEETIESLNQKVIALEKTKQRLSTEVEDLQIEVDRATAIANAAEKKQKAFDKIIGEWKLKVDDLAAELDAS 709
[Close]
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Match: UPI000C788FA4 (myosin heavy chain, muscle-like n=1 Tax=Eurytemora affinis TaxID=88015 RepID=UPI000C788FA4)

HSP 1 Score: 148 bits (374), Expect = 3.630e-38
Identity = 75/104 (72.12%), Postives = 89/104 (85.58%), Query Frame = 1 
Query:  211 GMGRIEELEGARAKLQAKIVENEELVDVLQTKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLSKASDVAAEVGASQDEGRNYSSELF 522
            GMGR+EELEG  +KL+++I E EE +D LQTK+AN EK+K R+G DLD+ISMEYERVHAAALITEKR KNFDKVLGEW +KASD+A EV AS +E RNYSSEL+
Sbjct: 1377 GMGRVEELEGNNSKLRSRITEAEETIDSLQTKLANIEKSKQRIGEDLDEISMEYERVHAAALITEKRGKNFDKVLGEWQAKASDIACEVEASNNECRNYSSELY 1480
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig103445.559.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1B6DZ47_9HEMI3.910e-4450.93Myosin_tail_1 domain-containing protein (Fragment)... [more]
B0W187_CULQU1.110e-4351.72Myosin-2 heavy chain n=7 Tax=Culex TaxID=53527 Rep... [more]
A0A8D8AKM5_CULPI2.830e-4351.72Myosin heavy chain, muscle (Fragment) n=4 Tax=Cule... [more]
UPI001954DA613.960e-4351.15uncharacterized protein LOC120424957 n=1 Tax=Culex... [more]
UPI000C7659AC7.140e-4358.16myosin heavy chain, muscle-like n=1 Tax=Eurytemora... [more]
A0A553PK51_TIGCA5.440e-4149.43Uncharacterized protein n=1 Tax=Tigriopus californ... [more]
A0A226DJW9_FOLCA3.050e-3949.10Myosin heavy chain, muscle n=1 Tax=Folsomia candid... [more]
A0A0K2T980_LEPSM2.660e-3845.98Myosin heavy chain 1 [Tribolium castaneum] n=3 Tax... [more]
A0A310SIQ5_9HYME3.500e-3849.38Myosin heavy chain, muscle n=1 Tax=Eufriesea mexic... [more]
UPI000C788FA43.630e-3872.12myosin heavy chain, muscle-like n=1 Tax=Eurytemora... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig103445contigEcto-sp8_S_contig103445:5..526 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeArthropoda
Stop0
Start0
Seed ortholog score168.7
Seed ortholog evalue2.4e-39
Seed eggNOG ortholog7176.CPIJ000848-PA
Preferred nameMhc
Model size522
KEGG koko:K10352,ko:K17751
KEGG Pathwayko04260,ko04261,ko04530,ko05416,map04260,map04261,map04530,map05416
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001667,GO:0002064,GO:0002065,GO:0002066,GO:0003006,GO:0003008,GO:0003012,GO:0003674,GO:0003824,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005694,GO:0005700,GO:0005703,GO:0005737,GO:0005856,GO:0006928,GO:0006936,GO:0006996,GO:0007010,GO:0007015,GO:0007275,GO:0007276,GO:0007281,GO:0007292,GO:0007297,GO:0007298,GO:0007517,GO:0007525,GO:0007527,GO:0008150,GO:0008307,GO:0009653,GO:0009888,GO:0009987,GO:0010631,GO:0010927,GO:0014866,GO:0015629,GO:0016043,GO:0016459,GO:0016462,GO:0016477,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0019953,GO:0022412,GO:0022414,GO:0022607,GO:0030016,GO:0030017,GO:0030029,GO:0030036,GO:0030154,GO:0030239,GO:0030241,GO:0030707,GO:0030855,GO:0030898,GO:0031032,GO:0031033,GO:0031034,GO:0031647,GO:0031672,GO:0032501,GO:0032502,GO:0032504,GO:0032956,GO:0032970,GO:0032989,GO:0032991,GO:0033043,GO:0034622,GO:0040011,GO:0042623,GO:0042692,GO:0042802,GO:0042803,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043254,GO:0043292,GO:0043519,GO:0043520,GO:0043933,GO:0044085,GO:0044087,GO:0044422,GO:0044424,GO:0044427,GO:0044430,GO:0044444,GO:0044446,GO:0044449,GO:0044464,GO:0044703,GO:0045214,GO:0046983,GO:0048468,GO:0048477,GO:0048513,GO:0048609,GO:0048646,GO:0048731,GO:0048856,GO:0048869,GO:0048870,GO:0050789,GO:0050794,GO:0050821,GO:0051128,GO:0051146,GO:0051179,GO:0051493,GO:0051674,GO:0051704,GO:0055001,GO:0055002,GO:0060361,GO:0060429,GO:0061061,GO:0065003,GO:0065007,GO:0065008,GO:0070925,GO:0071688,GO:0071689,GO:0071840,GO:0090130,GO:0090132,GO:0097435,GO:0098687,GO:0099080,GO:0099081,GO:0099512
Exons1
EggNOG free text desc.Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family
EggNOG OGs38CVC@33154,3BBPR@33208,3CSM1@33213,3SH7R@50557,41XGJ@6656,44X1M@7147,45HDQ@7148,COG5022@1,KOG0161@2759
Cds size522
COG Functional cat.Z
Best tax levelNematocera
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04147,ko04812
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143133.3420136-CDS-Ecto-sp8_S_contig103445:4..5261681143133.3420136-CDS-Ecto-sp8_S_contig103445:4..526Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig103445 5..526 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig103445.559.1prot_Ecto-sp8_S_contig103445.559.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig103445 5..526 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig103445.559.1

>prot_Ecto-sp8_S_contig103445.559.1 ID=prot_Ecto-sp8_S_contig103445.559.1|Name=mRNA_Ecto-sp8_S_contig103445.559.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=174bp
STQLEDTKALADAEAKDRAALLTKFKMMSTDLENLREKLENEAMRKSDAL
KALSKAQAEIQLWKSRYETEGMGRIEELEGARAKLQAKIVENEELVDVLQ
TKVANAEKTKGRLGSDLDDISMEYERVHAAALITEKRAKNFDKVLGEWLS
KASDVAAEVGASQDEGRNYSSELF
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mRNA from alignment at Ecto-sp8_S_contig103445:5..526+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig103445.559.1 ID=mRNA_Ecto-sp8_S_contig103445.559.1|Name=mRNA_Ecto-sp8_S_contig103445.559.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=522bp|location=Sequence derived from alignment at Ecto-sp8_S_contig103445:5..526+ (Ectocarpus species8 EcLAC_412)
AGTACGCAGCTGGAGGACACTAAGGCCCTTGCTGATGCGGAAGCTAAGGA
TCGTGCTGCACTCTTGACCAAGTTCAAGATGATGTCTACAGATCTCGAAA
ACTTGAGAGAAAAACTTGAAAATGAAGCCATGCGCAAGTCAGATGCACTG
AAGGCACTTTCCAAGGCTCAAGCAGAAATCCAACTGTGGAAATCCCGCTA
TGAGACTGAAGGTATGGGGCGCATTGAAGAACTTGAAGGTGCTCGTGCCA
AACTTCAAGCCAAAATTGTTGAGAATGAAGAGCTTGTTGATGTTCTTCAA
ACCAAAGTAGCCAATGCTGAAAAGACAAAGGGCAGACTTGGATCAGACCT
TGATGATATTTCAATGGAATATGAAAGAGTTCATGCTGCAGCCCTCATTA
CTGAGAAGCGAGCAAAGAACTTTGACAAGGTTCTTGGTGAATGGCTGTCT
AAGGCTTCAGATGTTGCTGCTGAGGTTGGTGCCTCTCAAGATGAAGGAAG
GAACTATAGCTCTGAATTGTTC
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig103445:5..526+

>mRNA_Ecto-sp8_S_contig103445.559.1 ID=mRNA_Ecto-sp8_S_contig103445.559.1|Name=mRNA_Ecto-sp8_S_contig103445.559.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=522bp|location=Sequence derived from alignment at Ecto-sp8_S_contig103445:5..526+ (Ectocarpus species8 EcLAC_412)
AGTACGCAGCTGGAGGACACTAAGGCCCTTGCTGATGCGGAAGCTAAGGA
TCGTGCTGCACTCTTGACCAAGTTCAAGATGATGTCTACAGATCTCGAAA
ACTTGAGAGAAAAACTTGAAAATGAAGCCATGCGCAAGTCAGATGCACTG
AAGGCACTTTCCAAGGCTCAAGCAGAAATCCAACTGTGGAAATCCCGCTA
TGAGACTGAAGGTATGGGGCGCATTGAAGAACTTGAAGGTGCTCGTGCCA
AACTTCAAGCCAAAATTGTTGAGAATGAAGAGCTTGTTGATGTTCTTCAA
ACCAAAGTAGCCAATGCTGAAAAGACAAAGGGCAGACTTGGATCAGACCT
TGATGATATTTCAATGGAATATGAAAGAGTTCATGCTGCAGCCCTCATTA
CTGAGAAGCGAGCAAAGAACTTTGACAAGGTTCTTGGTGAATGGCTGTCT
AAGGCTTCAGATGTTGCTGCTGAGGTTGGTGCCTCTCAAGATGAAGGAAG
GAACTATAGCTCTGAATTGTTC
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