mRNA_Ecto-sp8_S_contig10168.277.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig10168.277.1
Unique NamemRNA_Ecto-sp8_S_contig10168.277.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: D7FXL1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXL1_ECTSI)

HSP 1 Score: 265 bits (677), Expect = 5.580e-89
Identity = 136/139 (97.84%), Postives = 137/139 (98.56%), Query Frame = 1 
Query:  100 MSSNGEQSAGVSAPTLPTKRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKVTAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGFSEYHSPVLAK 516
            MSSNGEQSAGV+APTLPTKRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKVTAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYG SEY SPVLAK
Sbjct:    1 MSSNGEQSAGVAAPTLPTKRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKVTAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGLSEYRSPVLAK 139
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: D7FQI6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQI6_ECTSI)

HSP 1 Score: 64.3 bits (155), Expect = 2.010e-9
Identity = 30/102 (29.41%), Postives = 55/102 (53.92%), Query Frame = 1 
Query:  208 IGQGVLLTADVTGAELVVIRGCFEGKVTAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGFSEYHSPVLA 513
            +G+GV+LTA+VT  + VV+ G F+G +      + +G ++ G + C    + G      ++S +L +   A V+G ++Y  L+V  G +L G   ++S   A
Sbjct:  100 VGKGVVLTANVTSCDKVVVEGNFQGNIKTGTFVLSEGGQMEGEVRCSDGCIGGNMKATIIASKTLLIGKAAVVTGDMVYDSLQVLDGGRLQGKLTHYSSTTA 201
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: A0A6H5JYZ3_9PHAE (Reverse transcriptase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JYZ3_9PHAE)

HSP 1 Score: 64.3 bits (155), Expect = 5.340e-9
Identity = 29/102 (28.43%), Postives = 56/102 (54.90%), Query Frame = 1 
Query:  208 IGQGVLLTADVTGAELVVIRGCFEGKVTAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGFSEYHSPVLA 513
            +G+GV+LTA+VT  + VV+ G F+G +      + +G ++ G + C    + G      ++S +L +   A V+G ++Y  L+V +G ++ G   ++S   A
Sbjct:  226 VGKGVVLTANVTSCDKVVVEGNFQGNIETGTFVLSEGGQMEGEVRCSEGCIGGNMKATIIASETLLIGKAAVVTGDMVYDSLQVLEGGRIQGKLTHYSSSTA 327
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: A0A1L3J6P3_9FLAO (Uncharacterized protein n=1 Tax=Gramella salexigens TaxID=1913577 RepID=A0A1L3J6P3_9FLAO)

HSP 1 Score: 60.8 bits (146), Expect = 9.110e-9
Identity = 34/101 (33.66%), Postives = 52/101 (51.49%), Query Frame = 1 
Query:  208 IGQGVLLTADVTGAELVVIRGCFEGKV-TAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGFSEYHSPV 507
            I  G L+T +++G     I G  EG + T   + I +G  V+GT+EC+ A + GKF+G      +L L + AN+ G +I  +L V  GA      E +  V
Sbjct:   20 IAAGTLITGEISGKGCFRIEGTLEGSLKTPGKVVISEGGMVKGTLECDNADIEGKFNGKLFVKDTLSLRAPANIEGEVIAGKLAVEPGAVFNATCEMNGGV 120
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: UPI001F4DE4F3 (polymer-forming cytoskeletal protein n=2 Tax=Gramella TaxID=292691 RepID=UPI001F4DE4F3)

HSP 1 Score: 58.5 bits (140), Expect = 6.810e-8
Identity = 33/101 (32.67%), Postives = 49/101 (48.51%), Query Frame = 1 
Query:  208 IGQGVLLTADVTGAELVVIRGCFEGKV-TAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQLYGFSEYHSPV 507
            I  G L+T D+ G     + G  EG + T   + I +G  + GT+ECE A + GKF G  +    L L + AN+ G ++  +L V  GA      E +  V
Sbjct:   20 IAAGTLITGDIQGKGCFRVEGTLEGTLKTPGKVVISEGGLINGTLECENADIEGKFQGKLLIKGILTLRNTANIEGEVVTGKLAVEPGAVFNATCEMNGGV 120
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: UPI001F2A341E (polymer-forming cytoskeletal protein n=1 Tax=Aequorivita sp. F64183 TaxID=2874476 RepID=UPI001F2A341E)

HSP 1 Score: 56.6 bits (135), Expect = 5.680e-7
Identity = 36/111 (32.43%), Postives = 56/111 (50.45%), Query Frame = 1 
Query:  148 PTKRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKV-TAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQL 477
            P K+  +  +E     N+  I +G  L  D++ +    I G  EG V T   + + +  E++GT+ CE A + GKF+G    + +L L S A V G I+  +L V  GA L
Sbjct:    3 PEKKAKKNNVEPSTAQNR--ISEGTTLKGDISSSGFFRIDGSIEGNVNTPSKVVLGKTGEIKGTLSCENADLEGKFEGSLQVTGTLTLRSTALVEGEIVVGKLSVEPGAVL 111
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: A0A2D9QCI0_9FLAO (Cell shape determination protein CcmA n=1 Tax=Aequorivita sp. TaxID=1872390 RepID=A0A2D9QCI0_9FLAO)

HSP 1 Score: 51.2 bits (121), Expect = 5.470e-5
Identity = 36/109 (33.03%), Postives = 53/109 (48.62%), Query Frame = 1 
Query:  154 KRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKV-TAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQL 477
            K+  +  +E   T N+  I +G  L  DV+      I G  EG V T   + + +   + GT+ CE A + GKF+G    S +L L S A V G ++  +L V  GA L
Sbjct:    5 KKERKSNMEPGTTQNR--INEGTRLKGDVSSTGFFRIDGTIEGNVNTPSKVVLGKTGIIIGTLTCENADIEGKFEGNLQVSGTLSLRSTAVVEGDVVVGKLSVEPGATL 111
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BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Match: A0A6M2AS03_9FLAO (Polymer-forming cytoskeletal protein n=3 Tax=Aequorivita TaxID=153265 RepID=A0A6M2AS03_9FLAO)

HSP 1 Score: 51.2 bits (121), Expect = 5.640e-5
Identity = 32/91 (35.16%), Postives = 45/91 (49.45%), Query Frame = 1 
Query:  208 IGQGVLLTADVTGAELVVIRGCFEGKV-TAQCIDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRRLKVAKGAQL 477
            I +G  L  D++      I G  EG V T   + + +   + GT+ CE A + GKF+G    S +L L S A V G I+  +L V  GA L
Sbjct:   21 INEGTKLKGDISSTGFFRIDGTIEGNVHTPSKVVLGKTGVILGTLSCENADLEGKFEGSLQVSGTLTLRSTALVEGEIVVGKLSVEPGAVL 111
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig10168.277.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
D7FXL1_ECTSI5.580e-8997.84Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FQI6_ECTSI2.010e-929.41Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JYZ3_9PHAE5.340e-928.43Reverse transcriptase domain-containing protein n=... [more]
A0A1L3J6P3_9FLAO9.110e-933.66Uncharacterized protein n=1 Tax=Gramella salexigen... [more]
UPI001F4DE4F36.810e-832.67polymer-forming cytoskeletal protein n=2 Tax=Grame... [more]
UPI001F2A341E5.680e-732.43polymer-forming cytoskeletal protein n=1 Tax=Aequo... [more]
A0A2D9QCI0_9FLAO5.470e-533.03Cell shape determination protein CcmA n=1 Tax=Aequ... [more]
A0A6M2AS03_9FLAO5.640e-535.16Polymer-forming cytoskeletal protein n=3 Tax=Aequo... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig10168contigEcto-sp8_S_contig10168:218..3959 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Model size519
Hectar predicted targeting categoryother localisation
Exons6
Cds size519
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig10168.277.1prot_Ecto-sp8_S_contig10168.277.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig10168 218..3959 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143114.6600387-CDS-Ecto-sp8_S_contig10168:217..2881681143114.6600387-CDS-Ecto-sp8_S_contig10168:217..288Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 218..288 +
1681143114.6711314-CDS-Ecto-sp8_S_contig10168:815..8641681143114.6711314-CDS-Ecto-sp8_S_contig10168:815..864Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 816..864 +
1681143114.6867785-CDS-Ecto-sp8_S_contig10168:1764..18871681143114.6867785-CDS-Ecto-sp8_S_contig10168:1764..1887Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 1765..1887 +
1681143114.6961608-CDS-Ecto-sp8_S_contig10168:2497..25691681143114.6961608-CDS-Ecto-sp8_S_contig10168:2497..2569Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 2498..2569 +
1681143114.7038546-CDS-Ecto-sp8_S_contig10168:3179..32691681143114.7038546-CDS-Ecto-sp8_S_contig10168:3179..3269Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 3180..3269 +
1681143114.7130878-CDS-Ecto-sp8_S_contig10168:3845..39591681143114.7130878-CDS-Ecto-sp8_S_contig10168:3845..3959Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig10168 3846..3959 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig10168.277.1

>prot_Ecto-sp8_S_contig10168.277.1 ID=prot_Ecto-sp8_S_contig10168.277.1|Name=mRNA_Ecto-sp8_S_contig10168.277.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=173bp
MSSSENCTAALGGGSTKAREQGILASTDSTRSEMSSNGEQSAGVSAPTLP
TKRTVRQLLETRNTLNKMEIGQGVLLTADVTGAELVVIRGCFEGKVTAQC
IDIEQGAEVRGTIECEAARVNGKFDGVFMSSHSLHLASHANVSGRIIYRR
LKVAKGAQLYGFSEYHSPVLAK*
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mRNA from alignment at Ecto-sp8_S_contig10168:218..3959+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig10168.277.1 ID=mRNA_Ecto-sp8_S_contig10168.277.1|Name=mRNA_Ecto-sp8_S_contig10168.277.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=3742bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10168:218..3959+ (Ectocarpus species8 EcLAC_412)
ATGTCTTCGTCCGAGAATTGCACCGCCGCGCTTGGGGGGGGATCTACGAA
GGCAAGAGAACAGGGCATTTTGTTCCGTGATGATTTCCGATTGGCATGTA
GGACTCTCTATTGCTAGCATGGACCGGCTGACTAATGAACAGGTGTCCGT
GTCAACGCGGCTGAAATCAGGAAGTGCTTCTAAAGGCTACGTGATCCGGG
ACCCCCGTGAGGGGGACGCTAAGCTTCTCTCCAGTGTTGATGTTGGTGGT
TGATACATGTTGGTGCCTGGGTGGGATTGGCGTGATGATGTTACAGCTGA
CGTATTGATATTAATATTGAGGTTGACACGGTGGTGTTGCGGTTGATATG
ATGCTATTGTGTTTTTAGTGCGCTCTCGAGGGAGGTTAACGAAAGCAAGG
CGAGACACCAGGTGCACTCGTTTGTTCTTGCATCAGACTCCGTTATTCTA
CTCGGCGATTTGTTCAATCCACATAGTATACAACCTACCCCGTTTGTCCG
CGTCGTTGGGGGTACCGCCGAAGTAACCTCTGTCCCTACCTACCCCTATG
CCGTTGCTACATACACCACCGGTGCTTCCAGGCTGTCGAGCCGAAGAGAG
CTAGCACTGACAGCACTCGATCTGAGATGTCTTCGAACGGCGAACAGGTG
CGTGGATTCCTGTAGTAGGGCTTCGGATATTATTTTCGGAGGATTGTCTC
GATGAAAATTGGTACGCAACAAACGCCGTTCATTTTTTATTGACTCAAAT
ACTGATCCCAAAAGGATGCTTCTTGTCTGGGAATATTTTACGGCCTGTTA
TAGCATGGACAGCCCGTATCATGATTTGTTGCTTCCAATGTTGACTTCAA
AGCAAGGATTTTGTTTTTTGGGAACCTTGACGCCTACTCAGCATGGACAG
CCCGTAATGATTCCAAAAAAAGGAACGATTCCCCAAGAAAGGAAGCGTTT
TCTTTGGGAACGTTGGCGACATATTTTTGACTATAAGATGTGCAGCGCAG
CAGTCCCCGCGGTACGACGAAGCAGGGCCCCTTAGCGGCAGACACACCGC
CTTTTGTTGGAGTCGATATTTTTTTGGTATTAGGTTGCGGTAATTTTCGT
TGTGGGTATGGATGGGCAAGGGTAGTACTGTACAAAGCCGTATGCTCTCT
TCCTTGGTTGCAGTGGCATCACCGCCCTGTCCCGCCACCACTACCACCAC
CTGTCAACACCTTTTTTTCGCAGCAGCAGGCGTTTGTAGTAGTGGCGCAA
TTCACCCATTCGTAGTTGATACCCTCCTCAATCAAGTGAACACATCCCTC
CTTCCCTCTCTCTCACACATTCCTCGTATTTTCTCTTTTTTGCTTGCTCC
CTGTCTGATATGTTCCTCTCGCTTTCTCTCTTATATTTTCCTCTCGTTCT
CTCTCTGACATGTTCCTCTCGCTCTCTCATTCAAGGCAAAACAACGAAAA
ACAACAGATTTCTCGCATTTTCTCTTTTTTTGCGTGCTCCCTCTCTGACA
TGTTCCTCTCGCTCTCTCATTCAACGCAAATCAACGAACAACAATAGAGC
GCCGGCGTGTCGGCCCCTACACTGCCCACCAAGCGCACCGTACGGCAGCT
GCTGGAAACGAGGAACACGCTCAACAAGATGGAGATCGGGCAGGGGGTCC
TACTCACTGCTGACGTCACGGTAGGCCGCTCAGACGCTAGCCCTTGAGAT
CTTCCATCCTACCATTCTCGTGAATATGTGGTGTATCGATTCCAAGTGGT
TACCGGGAAAACTTGCTGGCGAATCAATCCATGGGTGTGCGATCGATTTT
TTTTAGGACCGATCGCTCCACGACGACCAGGAGGCTGATACCAAACCCTA
CGTTCACGCGCGAGGAAATTTCTCGGAAACATATTTCCGAAATGGCGCTT
TTTGGGCATGATAGACAGTCTTACTTATGTTGTGGTGCATTCGAGCTTTG
ATGGCACACACACCTGTGTGGTCGCCCTCAGCGATAGTTTTTTATTGTCA
GCACAAAGGTGAAAATTGTGTCGCGTTTATGTTTTTTTGCCGCAGCGGGG
GTGAGGTCTGTCTTCCTGAAATGTGTTTTCTTCTTTTTTTCTTGCTGCTG
AGGTGTCTGCTGCTCTGCGGAGTGTGTTTGTGCTCTATTTGGGTATGCGC
TTCCAGTTTTTGTTCGCGCGCGTCTGTGGGAGGCATGGTTATCGGGTGGT
GGCTCATCATGTCGGCTCTCCCTCCCTCAGCCTCCCCCCAATGTTGTTCC
ACGGACGTGCGCAACGTCGTGAAACGACAGGGAGCCGAGTTGGTGGTAAT
CAGGGGTTGTTTCGAAGGCAAGGTGACGGCACAGTGCATCGACATCGAGC
AGGTATGCGCATGCAGCTTGAAGCGTGACCACATGAAGCGTGCCCGAAAT
GAAAAACGGTGTGTCCCAGGTTGCCGAGAACATGATGAGCTGATGTCCTG
TAGCGCTGCAGAGGTTTTATTTTATCCCGGTCTCTTTTAACCGTGGAAGA
GATTAGTTCGTTTGTGTTTTACAGTCATTTTACTAGAAAACGGTCTCCCC
GGACGGCCGTCGACCATGCCAGCAGACGCAACGTTAACGATGAACCCCCT
CTCACATCACCAGCGTCCTTGTGCCATGTTGATTACCGGCAGGCATTCTC
ATCGCCCACGACAAGAGCACCATCACAAACAACAACAACAACAACAACAA
CAACAACAACAACAACAACAACAACAACAACAACAACAACAACAGCGAAC
CCTACCTTCTCTTCTACGTCTACGTCGCGGTAGCCTAGCCTACCTCCTCT
CCTCTTGCGCACCGAAACAATGGGCATCGGCACTTCACACCAACATCACA
ACAACAGCGACTCCTGCCTGTTCCTATACACCGAGCGCTTTGATAGCACC
CGCGTAGCCGATCTACTGTACCTATAACCTTGTGTCCTCCCGCGCAACAT
ATGCCTCCGCAGGGGGCGGAGGTGAGGGGGACGATCGAGTGCGAGGCGGC
ACGGGTCAACGGAAAATTCGACGGGGTGTTCATGTCTTCCCACTCCCTGC
ACGTGAGTATCTCGACTTTTTCTTTTTTTTTTCCCCAAGGGGCCGTTTCG
GTTGGTGGCCGCGGCTATGAAGGCGGTAGTATTTATATTGATAGCACGGC
AGATCATGTGTCCGTGCAAGGAAGGACCTCGCGATCTCTTCAATCGATAG
CATTGTTTTTCATCTTTGGAAGAGCCATTGAAACGTTAAGCAGCGTTGCT
TGCCCCTCCCCCGCCCGACCTCTGCCCCAACGATATTTGTACCGTCCCCC
CGCGCCTTCTTCGCTAGCAACATAAGTACCCGCCCCCTCCCCGTGACTCG
TTCTTCCTCACAGAGGCTCGCACCCACGCAAATTGTTGCGAACCGAAAGG
TTAGCTACCGTTTAGGCGATGTCCCGGCTCTTGCATTTCTGTGCTCGATT
TCCACCCCCCAGAGCGCGTACAACTCTTGAGGACGACTGGCTTAACACAC
AAAACGCCCCCCCAACCGTAGATTTTTTCACCACCCGTGTACTGTACGTA
CGATGCCCCCCGATGGCCGTGTGACTCTCTCCCTCCACTGCTGCCACCAC
CCCCGTGTCTGCTTTTCGCAATTCTCAGCTTGCCTCCCACGCCAACGTGT
CGGGACGCATCATCTACCGCCGCTTGAAGGTGGCGAAGGGCGCGCAACTC
TACGGCTTTTCGGAGTACCATTCGCCGGTGCTTGCGAAGTAG
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig10168:218..3959+

>mRNA_Ecto-sp8_S_contig10168.277.1 ID=mRNA_Ecto-sp8_S_contig10168.277.1|Name=mRNA_Ecto-sp8_S_contig10168.277.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=519bp|location=Sequence derived from alignment at Ecto-sp8_S_contig10168:218..3959+ (Ectocarpus species8 EcLAC_412)
ATGTCTTCGTCCGAGAATTGCACCGCCGCGCTTGGGGGGGGATCTACGAA
GGCAAGAGAACAGGGCATTTTAGCTAGCACTGACAGCACTCGATCTGAGA
TGTCTTCGAACGGCGAACAGAGCGCCGGCGTGTCGGCCCCTACACTGCCC
ACCAAGCGCACCGTACGGCAGCTGCTGGAAACGAGGAACACGCTCAACAA
GATGGAGATCGGGCAGGGGGTCCTACTCACTGCTGACGTCACGGGAGCCG
AGTTGGTGGTAATCAGGGGTTGTTTCGAAGGCAAGGTGACGGCACAGTGC
ATCGACATCGAGCAGGGGGCGGAGGTGAGGGGGACGATCGAGTGCGAGGC
GGCACGGGTCAACGGAAAATTCGACGGGGTGTTCATGTCTTCCCACTCCC
TGCACCTTGCCTCCCACGCCAACGTGTCGGGACGCATCATCTACCGCCGC
TTGAAGGTGGCGAAGGGCGCGCAACTCTACGGCTTTTCGGAGTACCATTC
GCCGGTGCTTGCGAAGTAG
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