mRNA_Ecto-sp8_S_contig101533.248.1 (mRNA) Ectocarpus species8 EcLAC_412

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp8_S_contig101533.248.1
Unique NamemRNA_Ecto-sp8_S_contig101533.248.1
TypemRNA
OrganismEctocarpus species8 EcLAC_412 (Ectocarpus species8 EcLAC_412)
Homology
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: UPI001592E9EB (MOSC domain-containing protein n=1 Tax=Parasphingorhabdus flavimaris TaxID=266812 RepID=UPI001592E9EB)

HSP 1 Score: 316 bits (809), Expect = 6.780e-108
Identity = 141/173 (81.50%), Postives = 154/173 (89.02%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            FGDDGE SAIAK  V   LMLGFEGL+GNQVGDPL HGGRDKA+HLYP+EHY FW+EKYPDV  L++ GAFGENFSC+GL+EDQLCLGD+FRVG ALIECSHARQPCWKLNHRFGK DVLKTVVKT KSGSYFRVLEEGRV+AGD+ +QQ RPYP+WPLD LF MIIGG HKG
Sbjct:   17 FGDDGELSAIAKDPVDRSLMLGFEGLEGNQVGDPLRHGGRDKAVHLYPIEHYDFWREKYPDVSELNETGAFGENFSCTGLTEDQLCLGDIFRVGSALIECSHARQPCWKLNHRFGKGDVLKTVVKTGKSGSYFRVLEEGRVKAGDDFVQQARPYPDWPLDWLFGMIIGGGHKG 189
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A2M7LCR3_9SPHN (MOSC domain-containing protein n=1 Tax=Sphingomonadales bacterium CG_4_10_14_3_um_filter_58_15 TaxID=1974093 RepID=A0A2M7LCR3_9SPHN)

HSP 1 Score: 310 bits (794), Expect = 1.310e-105
Identity = 134/173 (77.46%), Postives = 154/173 (89.02%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            FGDDGE SAIAK A    LMLGFEGL+GNQV DP+HHGGRDKA+HLYP EHY+FW++KYPD+ +LD  GAFGENFSC G++E  LCLGD+FRVGEALIECSHARQPCWKLNHRFGK DVLKTVVKT KSGSYFRVLEEGRV+AGD ++Q +RP+P+WPLDRLF +I+GG+HKG
Sbjct:   17 FGDDGEMSAIAKLATDQALMLGFEGLEGNQVADPVHHGGRDKAVHLYPAEHYSFWRKKYPDLDLLDSPGAFGENFSCEGMTESVLCLGDIFRVGEALIECSHARQPCWKLNHRFGKPDVLKTVVKTGKSGSYFRVLEEGRVRAGDSVVQVERPFPDWPLDRLFGLIVGGQHKG 189
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A2G2D8X2_9SPHN (MOSC domain-containing protein n=1 Tax=Sphingopyxis sp. TaxID=1908224 RepID=A0A2G2D8X2_9SPHN)

HSP 1 Score: 303 bits (776), Expect = 7.210e-103
Identity = 133/175 (76.00%), Postives = 152/175 (86.86%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKGHK 525
            FG +GE SAIAK      L LGFEGL+GNQV DPLHHGGRDKA+HLYP +HY FW+EKYPDV VLDQ GAFGENFSC+G++E QLCLGDVFR+G+A+IECSHARQPCWKLNHRFGK DVLKTV+KT KSGSYFRVLEEG+++AGD  IQ DRP P+WPLDRLF +IIGG+HKG +
Sbjct:   17 FGPEGEVSAIAKIPTDQSLFLGFEGLEGNQVADPLHHGGRDKAVHLYPDDHYPFWREKYPDVSVLDQPGAFGENFSCTGMTETQLCLGDVFRIGDAVIECSHARQPCWKLNHRFGKPDVLKTVIKTGKSGSYFRVLEEGQIKAGDIFIQLDRPLPDWPLDRLFGLIIGGQHKGQE 191
[Close]
BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A127MFG0_9SPHN (Molybdenum cofactor biosysynthesis protein n=1 Tax=Sphingorhabdus sp. M41 TaxID=1806885 RepID=A0A127MFG0_9SPHN)

HSP 1 Score: 301 bits (770), Expect = 5.910e-102
Identity = 130/173 (75.14%), Postives = 153/173 (88.44%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F DDGE SAI K A   P+MLGFEGL+GN+V DPLHHGGRDKA+HLYP+EHY FW+ +YPD+ +LD  GAFGENFSC+GL+E++LCLGDVFR+GEALIECSHARQPCWKLNHRFG+ DVLKTVVKT KSGSYFRVL+EG V+AGD+L+Q  RP P+WPLDRLF +I+GG+HKG
Sbjct:   17 FRDDGEFSAIVKNATVRPVMLGFEGLEGNEVADPLHHGGRDKAVHLYPIEHYAFWRGRYPDLDLLDAPGAFGENFSCAGLTEERLCLGDVFRLGEALIECSHARQPCWKLNHRFGETDVLKTVVKTGKSGSYFRVLQEGMVRAGDDLVQLARPLPDWPLDRLFGLIVGGQHKG 189
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: UPI001AA0A0CB (MOSC domain-containing protein n=1 Tax=Parasphingorhabdus sp. JHSY0214 TaxID=2806553 RepID=UPI001AA0A0CB)

HSP 1 Score: 297 bits (761), Expect = 1.540e-100
Identity = 131/173 (75.72%), Postives = 153/173 (88.44%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F +DGE SAIAK+ V   ++LG  GL GNQV DPLHHGGRDKA+HLYPVEHY FW++KYPD+ +L++ GAFGENFSCSGL+ED+LCLGDVFRVGEALIECSHARQPCWKLNH FG  DVLKTV+KT KSGSYFRVLE G+V+AGD++IQQDR +P+WPLDRLF +IIGG+HKG
Sbjct:   17 FREDGEMSAIAKQLVDQAIVLGPLGLAGNQVADPLHHGGRDKAVHLYPVEHYEFWKDKYPDLDILNRPGAFGENFSCSGLTEDKLCLGDVFRVGEALIECSHARQPCWKLNHHFGHPDVLKTVIKTRKSGSYFRVLETGKVRAGDDMIQQDRHHPDWPLDRLFGLIIGGKHKG 189
[Close]
BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A2D3RFX8_9SPHN (MOSC domain-containing protein n=2 Tax=Sphingomonadaceae TaxID=41297 RepID=A0A2D3RFX8_9SPHN)

HSP 1 Score: 297 bits (760), Expect = 2.180e-100
Identity = 130/173 (75.14%), Postives = 150/173 (86.71%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F DDG  SAIAK  V   L+LG EGL GNQV DPLHHGGRDKA+HLYP EHY FW++KYPD+ +L+Q GAFGENFSC+G++ED+LCLGD+FR+GEALI+CSHAR+PCWKLNHRFGK DVLKTVVKT KSGSYFRVLE G+VQAGD   QQ+RP P+WPLDRLF +IIGG+HKG
Sbjct:   17 FRDDGAMSAIAKHPVEDALILGLEGLAGNQVADPLHHGGRDKAVHLYPAEHYGFWRDKYPDLHLLEQPGAFGENFSCTGMTEDRLCLGDIFRLGEALIQCSHARKPCWKLNHRFGKPDVLKTVVKTGKSGSYFRVLETGKVQAGDLFFQQERPLPDWPLDRLFDLIIGGQHKG 189
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: UPI001E4D9561 (MOSC domain-containing protein n=1 Tax=Parasphingorhabdus litoris TaxID=394733 RepID=UPI001E4D9561)

HSP 1 Score: 289 bits (740), Expect = 2.190e-97
Identity = 126/173 (72.83%), Postives = 150/173 (86.71%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F +DGE SAIAK  V+  + LG  G +GNQV DPLHHGGRDKA+HLYP+EHY FW+ KYPDV VL++ GAFGEN SC+G++ED+LCLGDVFR+GEALIECSHARQPCWKLN+RFG  DVLKTV+KT KSGSYFRVLE G+V+AGD +IQQDRP+PEW L+RLF +IIGG+H+G
Sbjct:   17 FREDGEMSAIAKTPVTEAIALGLFGFEGNQVADPLHHGGRDKAVHLYPIEHYPFWKGKYPDVDVLNRPGAFGENLSCTGMTEDRLCLGDVFRLGEALIECSHARQPCWKLNYRFGHPDVLKTVIKTRKSGSYFRVLEPGKVRAGDRMIQQDRPFPEWSLERLFGIIIGGKHQG 189
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BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A653JG55_9SPHN (MOSC domain-containing protein n=1 Tax=Sphingorhabdus sp. 109 TaxID=2653173 RepID=A0A653JG55_9SPHN)

HSP 1 Score: 289 bits (739), Expect = 3.110e-97
Identity = 125/173 (72.25%), Postives = 144/173 (83.24%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F  DGE SAI K  V    MLG EGL GNQV D +HHGGRDKA+HLYP +HY FWQ++YPD+ +L+  GAFGENFSC+G+SED+LCLGD+FR+GEALI+CSHARQPCWKLNH FGK DVLKT+VKT KSG YFRVLE G+V+AGD+ IQQ RP PEWPLDRLF +IIGG HKG
Sbjct:   17 FRKDGETSAIVKMPVEDARMLGLEGLSGNQVADQVHHGGRDKAVHLYPADHYAFWQDRYPDLELLEHPGAFGENFSCTGMSEDRLCLGDIFRLGEALIQCSHARQPCWKLNHHFGKADVLKTIVKTGKSGCYFRVLETGKVRAGDDFIQQARPLPEWPLDRLFGLIIGGGHKG 189
[Close]
BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: UPI000D69FB85 (MOSC domain-containing protein n=1 Tax=Sphingorhabdus sp. EL138 TaxID=2073156 RepID=UPI000D69FB85)

HSP 1 Score: 287 bits (735), Expect = 1.550e-96
Identity = 122/173 (70.52%), Postives = 148/173 (85.55%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKG 519
            F +DGE SAIAK+ V+ P+ LG  GL+GNQV DPLHHGGRDKA+HLYPVEHY FW +KYP + +L + GAFGEN SC+G++ED+LCLGD+FR+G+ALIECSHARQPCWKLNH FG  DVLKTV+KT KSGSYFRVLE G++ AGD +IQQ+R +P+WPL RLF +IIGG+HKG
Sbjct:   20 FREDGEMSAIAKQLVAYPVQLGLLGLEGNQVADPLHHGGRDKAVHLYPVEHYAFWADKYPGLEILTRPGAFGENLSCTGMTEDKLCLGDIFRIGDALIECSHARQPCWKLNHHFGHPDVLKTVIKTTKSGSYFRVLEAGKISAGDTIIQQERIHPDWPLQRLFGLIIGGKHKG 192
[Close]
BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Match: A0A220W3R8_9SPHN (6-N-hydroxylaminopurine resistance protein n=1 Tax=Sphingorhabdus sp. SMR4y TaxID=2584094 RepID=A0A220W3R8_9SPHN)

HSP 1 Score: 283 bits (725), Expect = 4.200e-95
Identity = 124/175 (70.86%), Postives = 145/175 (82.86%), Query Frame = 1 
Query:    1 FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVEHYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIECSHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQDRPYPEWPLDRLFAMIIGGRHKGHK 525
            F DDG  SAIAK      LMLG EGL GNQVGDP+ HGG DKA+HLYP EHY FWQ +YPD+ +L+   AFGENFSCSG++E++LCLGD+FR+G ALI+CSHARQPCWKLNHRFG+ DV+KTV+KT KSGSYFRVLE G V+AGD  +QQ+RP PEWPLDRLF +IIGG HKGH+
Sbjct:   17 FRDDGIMSAIAKMPADGGLMLGREGLAGNQVGDPVRHGGLDKAVHLYPAEHYAFWQARYPDLALLEHPEAFGENFSCSGMTEERLCLGDIFRLGGALIQCSHARQPCWKLNHRFGRPDVMKTVMKTGKSGSYFRVLETGMVRAGDSFVQQERPQPEWPLDRLFNLIIGGGHKGHR 191
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp8_S_contig101533.248.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI001592E9EB6.780e-10881.50MOSC domain-containing protein n=1 Tax=Parasphingo... [more]
A0A2M7LCR3_9SPHN1.310e-10577.46MOSC domain-containing protein n=1 Tax=Sphingomona... [more]
A0A2G2D8X2_9SPHN7.210e-10376.00MOSC domain-containing protein n=1 Tax=Sphingopyxi... [more]
A0A127MFG0_9SPHN5.910e-10275.14Molybdenum cofactor biosysynthesis protein n=1 Tax... [more]
UPI001AA0A0CB1.540e-10075.72MOSC domain-containing protein n=1 Tax=Parasphingo... [more]
A0A2D3RFX8_9SPHN2.180e-10075.14MOSC domain-containing protein n=2 Tax=Sphingomona... [more]
UPI001E4D95612.190e-9772.83MOSC domain-containing protein n=1 Tax=Parasphingo... [more]
A0A653JG55_9SPHN3.110e-9772.25MOSC domain-containing protein n=1 Tax=Sphingorhab... [more]
UPI000D69FB851.550e-9670.52MOSC domain-containing protein n=1 Tax=Sphingorhab... [more]
A0A220W3R8_9SPHN4.200e-9570.866-N-hydroxylaminopurine resistance protein n=1 Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp8_S_contig101533contigEcto-sp8_S_contig101533:15..539 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species8 EcLAC_412 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeBacteria
Stop0
Start0
Seed ortholog score221.5
Seed ortholog evalue3.1e-55
Seed eggNOG ortholog1522072.IL54_0942
Preferred nameyiiM
Model size525
Hectar predicted targeting categoryother localisation
Exons1
EggNOG free text desc.protein conserved in bacteria
EggNOG OGs1RAPM@1224,2K3XH@204457,2U5IG@28211,COG2258@1,COG2258@2
Cds size525
COG Functional cat.S
Best tax levelSphingomonadales
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681143112.9438539-CDS-Ecto-sp8_S_contig101533:14..5391681143112.9438539-CDS-Ecto-sp8_S_contig101533:14..539Ectocarpus species8 EcLAC_412CDSEcto-sp8_S_contig101533 15..539 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp8_S_contig101533.248.1prot_Ecto-sp8_S_contig101533.248.1Ectocarpus species8 EcLAC_412polypeptideEcto-sp8_S_contig101533 15..539 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp8_S_contig101533.248.1

>prot_Ecto-sp8_S_contig101533.248.1 ID=prot_Ecto-sp8_S_contig101533.248.1|Name=mRNA_Ecto-sp8_S_contig101533.248.1|organism=Ectocarpus species8 EcLAC_412|type=polypeptide|length=175bp
FGDDGEKSAIAKKAVSSPLMLGFEGLDGNQVGDPLHHGGRDKAIHLYPVE
HYTFWQEKYPDVRVLDQAGAFGENFSCSGLSEDQLCLGDVFRVGEALIEC
SHARQPCWKLNHRFGKRDVLKTVVKTAKSGSYFRVLEEGRVQAGDELIQQ
DRPYPEWPLDRLFAMIIGGRHKGHK
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mRNA from alignment at Ecto-sp8_S_contig101533:15..539-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp8_S_contig101533.248.1 ID=mRNA_Ecto-sp8_S_contig101533.248.1|Name=mRNA_Ecto-sp8_S_contig101533.248.1|organism=Ectocarpus species8 EcLAC_412|type=mRNA|length=525bp|location=Sequence derived from alignment at Ecto-sp8_S_contig101533:15..539- (Ectocarpus species8 EcLAC_412)
TTTGGCGATGATGGTGAAAAAAGCGCGATTGCGAAGAAAGCGGTATCAAG
CCCGCTGATGCTCGGGTTTGAAGGACTGGACGGTAACCAGGTCGGTGATC
CGCTGCATCATGGTGGACGGGACAAGGCAATCCATCTCTATCCGGTCGAA
CATTATACCTTTTGGCAAGAGAAATATCCCGATGTGCGGGTGCTTGACCA
AGCTGGAGCATTTGGCGAGAATTTTAGCTGCTCGGGATTGAGCGAGGATC
AATTGTGTCTTGGTGATGTCTTTCGGGTCGGGGAAGCGTTGATCGAGTGC
AGCCATGCTCGGCAACCATGTTGGAAGCTAAACCATCGGTTTGGCAAACG
TGATGTGCTGAAGACGGTAGTGAAAACTGCGAAATCCGGCAGCTATTTTC
GCGTTTTGGAAGAAGGCAGGGTGCAAGCCGGAGACGAACTGATCCAGCAA
GATCGCCCCTATCCCGAATGGCCGCTGGACCGGCTATTTGCCATGATTAT
TGGCGGTCGGCACAAGGGCCACAAA
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Coding sequence (CDS) from alignment at Ecto-sp8_S_contig101533:15..539-

>mRNA_Ecto-sp8_S_contig101533.248.1 ID=mRNA_Ecto-sp8_S_contig101533.248.1|Name=mRNA_Ecto-sp8_S_contig101533.248.1|organism=Ectocarpus species8 EcLAC_412|type=CDS|length=525bp|location=Sequence derived from alignment at Ecto-sp8_S_contig101533:15..539- (Ectocarpus species8 EcLAC_412)
TTTGGCGATGATGGTGAAAAAAGCGCGATTGCGAAGAAAGCGGTATCAAG
CCCGCTGATGCTCGGGTTTGAAGGACTGGACGGTAACCAGGTCGGTGATC
CGCTGCATCATGGTGGACGGGACAAGGCAATCCATCTCTATCCGGTCGAA
CATTATACCTTTTGGCAAGAGAAATATCCCGATGTGCGGGTGCTTGACCA
AGCTGGAGCATTTGGCGAGAATTTTAGCTGCTCGGGATTGAGCGAGGATC
AATTGTGTCTTGGTGATGTCTTTCGGGTCGGGGAAGCGTTGATCGAGTGC
AGCCATGCTCGGCAACCATGTTGGAAGCTAAACCATCGGTTTGGCAAACG
TGATGTGCTGAAGACGGTAGTGAAAACTGCGAAATCCGGCAGCTATTTTC
GCGTTTTGGAAGAAGGCAGGGTGCAAGCCGGAGACGAACTGATCCAGCAA
GATCGCCCCTATCCCGAATGGCCGCTGGACCGGCTATTTGCCATGATTAT
TGGCGGTCGGCACAAGGGCCACAAA
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