mRNA_Ecto-sp6_S_contig103.904.1 (mRNA) Ectocarpus species6 EcLAC_371

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp6_S_contig103.904.1
Unique NamemRNA_Ecto-sp6_S_contig103.904.1
TypemRNA
OrganismEctocarpus species6 EcLAC_371 (Ectocarpus species6 EcLAC_371)
Homology
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BLAST of mRNA_Ecto-sp6_S_contig103.904.1 vs. uniprot
Match: D7G0U1_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0U1_ECTSI)

HSP 1 Score: 392 bits (1006), Expect = 4.340e-136
Identity = 237/252 (94.05%), Postives = 243/252 (96.43%), Query Frame = 1 
Query:    1 MDQDGVFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQCVKPDQAITIYKLHDHETNEDYLEFDEEVIFQTACRMCLNIKSCLREGHKLIDWRPTFAGGRVAGSSHPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPGRAPRSARGRSASTSVVDDEMEVVEDYGDGGGSGRKVFPAGGRGRKGRTXXXXXXXXXGVAPQESTATRRGPHKRKSPASSAYPRSASSHQLVQLKVCYC 756
            MDQDG+FLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQCVKPDQAITIYKLHDHETNEDYLEFDE++IFQTACRMCLNIKSCLREGHKLIDWRPTFAGGRVAGSSHPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  PGRAPRSARGRSASTSVVDDEMEVVEDYGDGGG+GRKVFPAGGRGR+G  XXXXXXX     PQESTATRRGPHKRKSPASSAYPRSASSHQL+QLKVCYC
Sbjct:    1 MDQDGLFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQCVKPDQAITIYKLHDHETNEDYLEFDEKIIFQTACRMCLNIKSCLREGHKLIDWRPTFAGGRVAGSSHPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--PGRAPRSARGRSASTSVVDDEMEVVEDYGDGGGTGRKVFPAGGRGRRGHAXXXXXXX---XXPQESTATRRGPHKRKSPASSAYPRSASSHQLMQLKVCYC 247
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BLAST of mRNA_Ecto-sp6_S_contig103.904.1 vs. uniprot
Match: A0A6H5LER2_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LER2_9PHAE)

HSP 1 Score: 202 bits (515), Expect = 5.820e-58
Identity = 99/115 (86.09%), Postives = 103/115 (89.57%), Query Frame = 1 
Query:    1 MDQDGVFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQCVKPDQAITIYKLHDHETNEDYLEFDEEVIFQTACRMCLNIKSCLREGHKLIDWRPTFA-GGRVAG 342
            MDQDG+FLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELT SHRQCVKPDQAITIYKLHDHETNEDYLEFDEE+IFQTACRMCLNIKSCLR G     +  TF  GG+ AG
Sbjct:    1 MDQDGLFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTNSHRQCVKPDQAITIYKLHDHETNEDYLEFDEEIIFQTACRMCLNIKSCLRLGKV---FSATFGKGGKSAG 112
[Close]
BLAST of mRNA_Ecto-sp6_S_contig103.904.1 vs. uniprot
Match: D7G0T9_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7G0T9_ECTSI)

HSP 1 Score: 118 bits (295), Expect = 1.370e-26
Identity = 51/113 (45.13%), Postives = 73/113 (64.60%), Query Frame = 1 
Query:    1 MDQDGVFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQCVKPDQAITIYKLHDHETNEDYLEFDEEVIFQTACRMCLNIKSCLREGHKLIDWRPTFAGGRVA 339
            MD DG  L++  CN C  NK   + C+ R+HN R++ V+ DEL   HRQCVKPDQ +TI+KL D  T E+ +E+  ++IFQ  C  C+++K+C+  GH+L DW P    GR +
Sbjct:    1 MDVDGTCLVVSPCNNCRINKNGVQACIMRKHNARLTAVKRDELAARHRQCVKPDQTMTIFKLVDRNTGEESVEYSPDMIFQRPCASCIHLKTCILGGHELTDWTPEGGRGRAS 113
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp6_S_contig103.904.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 3
Match NameE-valueIdentityDescription
D7G0U1_ECTSI4.340e-13694.05Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5LER2_9PHAE5.820e-5886.09Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7G0T9_ECTSI1.370e-2645.13Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp6_S_contig103contigEcto-sp6_S_contig103:225235..230819 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species6 EcLAC_371 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Model size759
Hectar predicted targeting categoryother localisation
Exons6
Ec32 ortholog descriptionhypothetical protein
Ec32 orthologEc-23_001500.1
Cds size759
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp6_S_contig103.904.1prot_Ecto-sp6_S_contig103.904.1Ectocarpus species6 EcLAC_371polypeptideEcto-sp6_S_contig103 225235..230819 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682348741.4114578-CDS-Ecto-sp6_S_contig103:225234..2252641682348741.4114578-CDS-Ecto-sp6_S_contig103:225234..225264Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 225235..225264 -
1682348741.42221-CDS-Ecto-sp6_S_contig103:225396..2254801682348741.42221-CDS-Ecto-sp6_S_contig103:225396..225480Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 225397..225480 -
1682348741.4318037-CDS-Ecto-sp6_S_contig103:225740..2261011682348741.4318037-CDS-Ecto-sp6_S_contig103:225740..226101Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 225741..226101 -
1682348741.4414732-CDS-Ecto-sp6_S_contig103:226707..2268261682348741.4414732-CDS-Ecto-sp6_S_contig103:226707..226826Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 226708..226826 -
1682348741.4501529-CDS-Ecto-sp6_S_contig103:228197..2283021682348741.4501529-CDS-Ecto-sp6_S_contig103:228197..228302Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 228198..228302 -
1682348741.459715-CDS-Ecto-sp6_S_contig103:230759..2308191682348741.459715-CDS-Ecto-sp6_S_contig103:230759..230819Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig103 230760..230819 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp6_S_contig103.904.1

>prot_Ecto-sp6_S_contig103.904.1 ID=prot_Ecto-sp6_S_contig103.904.1|Name=mRNA_Ecto-sp6_S_contig103.904.1|organism=Ectocarpus species6 EcLAC_371|type=polypeptide|length=253bp
MDQDGVFLMLKMCNGCSHNKGSWKKCMSRRHNPRMSTVREDELTYSHRQC
VKPDQAITIYKLHDHETNEDYLEFDEEVIFQTACRMCLNIKSCLREGHKL
IDWRPTFAGGRVAGSSHPPPSPPASPPAPTPPPPPPPPADRGGGARRGGG
GPGRAPRSARGRSASTSVVDDEMEVVEDYGDGGGSGRKVFPAGGRGRKGR
TAAAAAAAAAGVAPQESTATRRGPHKRKSPASSAYPRSASSHQLVQLKVC
YC*
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mRNA from alignment at Ecto-sp6_S_contig103:225235..230819-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp6_S_contig103.904.1 ID=mRNA_Ecto-sp6_S_contig103.904.1|Name=mRNA_Ecto-sp6_S_contig103.904.1|organism=Ectocarpus species6 EcLAC_371|type=mRNA|length=5585bp|location=Sequence derived from alignment at Ecto-sp6_S_contig103:225235..230819- (Ectocarpus species6 EcLAC_371)
ATGGACCAGGATGGCGTGTTCCTCATGCTCAAGATGTGCAACGGCTGCAG
CCACAACAAGGTGTGTGAGAGAGGCAGGCTCACACGGTTAACCCTTGTAC
GCCCGCCAATGTTTGTCTTTTCGTCTTTCGACATCCTGTCTCACCGTGTC
TCCCCTGCTGGCACGCACCCTCGGCGCTTGTTTTCGTTGTTGTGGTGGCA
TAGGCCGTACCCACCAGCAGGGTAAGCTCAGCCTGTAGTCCTGACCGGAG
GTCGACTTCGAATGGTGTCGATGGTCAATGTTACGATGGGGTCGCGTTTA
CCTGTCGAACACACGTTTCGAGGAATGACCCTTTGGTGCCGAGGGCGAGA
ATCGGGCACACACACCAGACGGGCAGGCAGCTCGGGGAGACTGATCGATC
CCTTTCTCATGTCTCGGCACTCCTCTCTACCGACACACACCAGTAGTCTA
ATGTCCTCGCACTACTGAGGCAGAGGACAACGACAATTAGGATGCTCTCT
CGGTGCGAATGGCGAGAACGACGACACTGCCGCTGCTGCTGCTGCTCGTT
CTATAGCAGTTTATGCATATCACACTTTTTAAAGCGGACAAGCTGCCCGA
TTGTGCCGTGCTGCCTCGTCGATTGTACCCCGCCTCCGCCTCGCTTCGCT
CGACAACACACAGCAGCAGTAGGGATCACACAAACCTAGAAGAGGGGGGA
GAGCTGAGCTTCATGTGTTGCTCGCTCCTTCCTATGCCGAGAAGTCGATG
GGGAGGGTTGTCCTGTTGAGACTCGAACTGCTGCCGCCGAGTCCCTCGAT
CGACCAATAAAACACCCTCTGTTGCCTCCAATTAAACATAACGCCGGCCG
TCGCAGCCGTCGAGGTTCAACGCCAACACGGTTATCATCTCACCCCTTCC
CAACCAATCCTGTACGCGAGCCTGCCCTAGAGGTCTGGCCGGTGAAGATG
GTCGGCCTAATGCTGTTGTTGTGTGTGGACGCGATGCGCTGAGCCGGGCA
GCGGCAGCGGTCAAATTCATCGTCAACCCTCCCATCCCCCACGCACGCGC
TCGTCGGACAATCGTCCAAAAACAAAATCGAGCACGTTCATCAAGAGTAC
ATTCGGTACTCCACTCACTGAAAAGACTCCGCCACATCCCGTTCGACTGT
GGGTATATCTCGAATGGGTAGTCTTTCTGTATGTTTTTTTAATATTCCTT
AATACGGTATGTACTTGGAGTACAGTAGTTGTGTGGCCGGATTCGCGTGT
TTGCTAATGAAGCCCGGCGTTAGGAGTGCAAACCTATATCGCATGGTGTT
GGCCTATCATCACGTCGCAAGATGGCGTACTTTTGAAATGACGCTACAAG
CGAGCTGCTTCCAAAAACATCAGGTTGTAGACTAGGCCTGTAGACTACAA
TGAGTAGCACACCCGTCTCCGGTAAGGCGATGTATTCAAAAACAGCAGTC
AAGCAGCACCCTGCTTCCTATTCATGGACGAAAGAGACCACCAGCTAGGA
AGATTTTTTTTTTTTTTTCAAAGTACAATTTACGGCGGGGTGAAGGACAC
CGCCCCGGCGTTGTGTCGCGTGGACCCACCTACATAGTCTTGCCAGGGGA
GGGGTCCACACACACCCTTTTTTCCATGTGGCATGTACCATGCTGCAGTA
CCAGTGCTTTTGCTTTTGTTCAAGTCACCAGGGTCTGGTTATTCCCATCT
TAGCCTCGCTCTTTCAAAGGCAGGAGGGGAGGGTAGGGGTGACTCAGGGA
AAGGAACCCTTGTGTGTGACCACGGGCCGGAGCTCCTAGCTACCCGGCAG
TGATGAGAGCGAAACACAAAAACGTTCGTCAATCGTCCGGGATTGGAGAT
CTAAACATTCCGCGACGGTACATACCTGCCTATCAAGAGGGTGGCCCTTG
GCTTAGACGGGAGGCCCTTCGAAACCATACTCTTTCTTCACAAGACCCTT
TGTTTGGAGGTTTGAAACAACGAAGACCGCACGAGCGACAGCGAGGGTAC
CTCTCATTTCGCGTGTAACGCATGTTACGGGTTATAGGATTGAACCTGTA
CGAGCCATCAACGCGAAACGCACGGCATCGCTCCACTTTCTGGGCAATGA
GGGACGACTGCTGCCCGCTCCCTTCGACGGCGGTCTTTGCGTGGCGTCTG
CACAACACATGACGGGAAGGCCGGTTGTTTAACATTCCCAATCGATGGCT
TCTCAGGGTTAACTGTAAGAGTGTTGCCACCACCCCATTATGAAGGCGAT
TGTGCGTGTGCTGGTTGCCTGAACTCGTTTCGATGGGCTTATTATCTGCT
CTCATTGCTGTTTAGAAAAAGACACATGTAATCGCAGAAAGGACTTGGTA
CGGCCGTTCGTGATGTAGCCACAGCATTTTCTACGATTTCCACAGATGAT
TGTTATTTTTTCGACCGCCCCTGTTCGTCTTTCTTTCTTTCTTCTTTTTG
CTCTCGACTGCGTATTCATCTTTATTTATTTCTGTTTTCGACCACGGCGG
CGGCGGCGCCCTCGCAGGGGAGCTGGAAGAAGTGCATGTCAAGGAGGCAC
AACCCCAGGATGTCCACGGTCCGTGAGGACGAGCTGACGTATAGCCACCG
ACAGTGCGTCAAGCCGGACCAGGTTAGCAACTCGTCGTTTCATTTCCACG
TTCCATGTCCTCCTTAGCAGCTTGGTTTTGAAGCAGTTTTTTTTAGCTCA
GGGCTGGCTATTTTGCCCTAATGTAGCCGTGTTCTCTTTCAGCTTTGTCT
TGAAGCAAGAGAGTGAGCTCGTCGGGGCTGGTTTTTTGTCTTAATGTACC
GGGTTCTATTAACAGCCCTGTCTTGAAGCAGTTCCGCTCTTACACAGAGC
TAGTCTGGGCTGGATAATTTTTTGGTCTTGTCGTCCGCTTTTAGAGAGCT
AGCTGGTTGGGGCTGCGGTTCTTTGTTGTGTTGTCGTGGCTCGGCTTTTT
CCGGCTCGGTTCTCTTTCACATTTTTTTTGTTTTCTTGCAGTTAGTTCCG
TGTGGAGAGCTCGACGGGGGCGGTCATTTTGTCTCTTCGTGTGGGCCGGG
TTGTTTCACCCGCTCGTCGCCGGGTGCTCCGGCTGGGGCGTCGCGGCTCA
AGCACCCTAGACCTGTCGGCCACGAGAACCTGGGCTACTGACTGCTGTTC
GTGGAGCACGCCTACCCACCGCCCCCCCCGACATACCCTTGCATACACTC
CCCCCATCAGCACTTTGCTGCACTGCTGCTGTAGAGCAACATGGTACCAT
GGAGGGGACTGTTCGGCGCCGGTTTATTGTATTGTGCCGGTATTTTCGGC
GGGCACGCACCGAACTACCAGGTCATGCCGTGCTTTGAATAGTGCCGGTG
TCCGGCATCGTGCCGGTGTCCGGTATCGTGCCTATGTCCGGTATAGTGCC
GGTGTCCCGCATAGTGCCGGTGTCCCGCATAGTGCCGGTGTCCGGTATAG
TGCCGGTGTCCCGCATTGTGCCGGTGTCCCGCATTGTGCCGGTGTCCCGC
ATAGTGCCGGTGTCCCGCATAGTGCCGGTGTCCGGTTTTGTGCCGGGCCA
TTCTCTGGGCACATGTACCGGCTCCGAACAGCTCCCCACACTGTAGTCGT
TTTTCGAAGCTTGTCCCGGTCCCCGGACCGCAGTTCGGTCGATTTTTTTT
ATTGCCCTCCTGTCAAAATTGGTGCTGCTTGCTCGTTCTTTTCGGATCCG
TGGATCCTCGAAATGACTCCCTTGAAAATATACAGCCCAGGTGTATGGCT
TCTACGATGCCGATGTTTTGTACGTGGAAGCTTGCTGAGGCAGCTGTAAT
TTTGCCGTCCCCTGGCTTTGACGCTTAGGCTTTTCTCACGACTACACGGG
GTGTGCCAGGTGTAGGCCTTCTACGAAGCCGATTTTTTTACGTACAAGCT
TGCTGAGGCAACTTTGATTTGACGCGACTCTCGCGCCTTCTCAGCCTGCG
CTCTTTCCCGCTATGCCGTCCCCTATCCCCTACCCCACGCCAGGCCATCA
CCATCTACAAACTCCACGACCACGAGACGAACGAGGACTACCTGGAGTTC
GACGAGGAGGTTATCTTTCAGACGGCGTGCCGCATGTGTCTCAACATCAA
GTCCTGCCTCAGGTGCGAAAAAAGACATGCGCGCTGGACGCCGTGCGGAT
GTAGCCGGGGTGTCTGTGCGGTTTTAGGGTTGGGAATAGTCAAGGTAAGG
TTGGGACCGCTTAGAAGAGTCAAGGCAACAACAACAAAAACAACAAAAAC
AACAACAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAACCG
TTGTATGCAATTATCAACAAGAACTGCTGTCGGTGGGTAGATCGCTCTAG
GTTGTCCCGGCCCGATCTTGCGCAATATATCGTAGGACGATATCGGTTCG
CCAGATCGTTATGGTACTCAATCCACATTCCAAAAAGTTTTTCTCGGACA
AAGGCGACCCCTCGGATCAACCATCGTAGTCGATTTTTTTTTTCGAATTA
TTTAGCCAGGCAAATGTACCCGATTTCTCTGATCCTGCGTTGAGCCTGAT
AATCCTCCTAGTTCGTCGTCTTTGGCTCTGCTGGAAATCTTACGTTGGGC
CTGATAGCCTCCAATTTGGCTGCTTTGCCTCTTCCCTCTTTCTTTTTTTT
GTCCCTCCCCCTTTTTGTTCTTCACTCGCTGTTGTCGTGGTCGTTTTTTG
TCATTGCTTTGCTCTCAGGGAGGGACACAAGCTCATCGACTGGAGGCCAA
CCTTCGCCGGAGGCAGGGTGGCCGGCTCCTCGCACCCCCCCCCCAGCCCC
CCCGCCTCTCCCCCCGCGCCTACGCCGCCGCCGCCGCCGCCGCCGCCCGC
GGACCGTGGCGGTGGAGCGAGGAGGGGTGGCGGGGGCCCCGGCAGGGCGC
CGAGGAGCGCTAGAGGGAGGTCAGCAAGCACCTCGGTAGTGGACGACGAG
ATGGAGGTTGTCGAAGATTATGGTGATGGTGGTGGTAGTGGCCGGAAGGT
GTTCCCTGCGGGCGGAAGGGGGCGGAAGGGACGTACTGCTGCTGCTGCTG
CTGCTGCTGCTGCTGGCGTTGCTCCGCAGGTGTGTTGTTGTGCCTGTCAG
GTGTTTTGGGTGTTTGTGTTTAGGTTTCTTGACTTGGAAACCTGTGTGTG
TGTGTGTGTTTTTTTGTATCTCCTACTTTTTTCTGCTCGAACCCCCATGA
GTTGTGTTGAGTTCTCTCTCTCTCGTTCGATCTAGTCCCGTGAAACCATG
ACAGTTACCAAGACATCATTTGATCCCTGTTTTTATGCCCTGCCCCTCCC
CCCGCCGCTTTATTTTCGCTCCATGTTTGCTTGGAAAAGGAGTCGACGGC
GACGCGTAGGGGTCCTCACAAGCGCAAGTCGCCGGCCTCTTCCGCGTACC
CGCGGTCCGCGAGCTCGCATCAGGTGACCATGCCGTGCACACAACCATCG
TTTGTTTGTTTTTCTAGTCTTTCCCCTCCCCTTTTTGGAAAATTGTCATG
CTGGCCTTTGTCCTCACAATGCTCTAAACAGCGACCGGTCTGTGTTTAAC
TCTAGCTAGTGCAGCTGAAGGTGTGCTATTGTTAG
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Coding sequence (CDS) from alignment at Ecto-sp6_S_contig103:225235..230819-

>mRNA_Ecto-sp6_S_contig103.904.1 ID=mRNA_Ecto-sp6_S_contig103.904.1|Name=mRNA_Ecto-sp6_S_contig103.904.1|organism=Ectocarpus species6 EcLAC_371|type=CDS|length=759bp|location=Sequence derived from alignment at Ecto-sp6_S_contig103:225235..230819- (Ectocarpus species6 EcLAC_371)
ATGGACCAGGATGGCGTGTTCCTCATGCTCAAGATGTGCAACGGCTGCAG
CCACAACAAGGGGAGCTGGAAGAAGTGCATGTCAAGGAGGCACAACCCCA
GGATGTCCACGGTCCGTGAGGACGAGCTGACGTATAGCCACCGACAGTGC
GTCAAGCCGGACCAGGCCATCACCATCTACAAACTCCACGACCACGAGAC
GAACGAGGACTACCTGGAGTTCGACGAGGAGGTTATCTTTCAGACGGCGT
GCCGCATGTGTCTCAACATCAAGTCCTGCCTCAGGGAGGGACACAAGCTC
ATCGACTGGAGGCCAACCTTCGCCGGAGGCAGGGTGGCCGGCTCCTCGCA
CCCCCCCCCCAGCCCCCCCGCCTCTCCCCCCGCGCCTACGCCGCCGCCGC
CGCCGCCGCCGCCCGCGGACCGTGGCGGTGGAGCGAGGAGGGGTGGCGGG
GGCCCCGGCAGGGCGCCGAGGAGCGCTAGAGGGAGGTCAGCAAGCACCTC
GGTAGTGGACGACGAGATGGAGGTTGTCGAAGATTATGGTGATGGTGGTG
GTAGTGGCCGGAAGGTGTTCCCTGCGGGCGGAAGGGGGCGGAAGGGACGT
ACTGCTGCTGCTGCTGCTGCTGCTGCTGCTGGCGTTGCTCCGCAGGAGTC
GACGGCGACGCGTAGGGGTCCTCACAAGCGCAAGTCGCCGGCCTCTTCCG
CGTACCCGCGGTCCGCGAGCTCGCATCAGCTAGTGCAGCTGAAGGTGTGC
TATTGTTAG
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