mRNA_Ecto-sp6_S_contig98.18776.1 (mRNA) Ectocarpus species6 EcLAC_371

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp6_S_contig98.18776.1
Unique NamemRNA_Ecto-sp6_S_contig98.18776.1
TypemRNA
OrganismEctocarpus species6 EcLAC_371 (Ectocarpus species6 EcLAC_371)
Homology
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BLAST of mRNA_Ecto-sp6_S_contig98.18776.1 vs. uniprot
Match: D7G0U8_ECTSI (Phosphatidylinositol-4-phosphate 5-kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0U8_ECTSI)

HSP 1 Score: 267 bits (683), Expect = 2.500e-81
Identity = 293/359 (81.62%), Postives = 299/359 (83.29%), Query Frame = 1 
Query:    1 MIVKYEGAIHCGTMEGQGRAEYDDGEA------------------RMWKNGMRHGTGTLTFTNGSTYSGSWDKDXXXXXXXXXXXXXXXXXXXXXXXXXXXKDGMMSXXXXXXXXXXXXXXXXXXXXXXXXXXRQTFASGGGGGGRGDEPEKVAATGGENEWYDGQWKAGSFHGQGEYHFADSGDVYVXXXXXXXXXXXXXXXXXXXXXXXXXDLWCDLRHGHGIHSGPDSTXXXXXXXXXXXXXXKLSDKDGNVFEGFFREXXXXXXXXXXXXXXXXXXXXXXXXXXXKGRRSGEGTMFAAKTXXXXXXXXXXXXXXXXXRLRLPCGASVEGLWILGNLVGNSKFSFAPDSEWNDPSY 1023
            MIVKYEGAI+ GTMEGQGRAEYDDGE                                                XXXXXXXXXXXXXXXXXXXXXXXXXXX      XXXXXXXXXXXXXXXXXXXXXXXXXXRQTFASGGGGGGRGDEPEKVAATGGENEWYDGQWK+G+FHGQGEYHFA+SGDVYVXXXXXXXXXXXXXXXXXXXXXXXXX LWCDLRHGHG+HSGPDST   XXXXXXXXXXXKLSDKDGNVFEGFFREXXXXXXXXXXXXXXXXXXXXXXXXXX +GRRSGEGTMFAAKTXXXXXXXXXXXXXXXXXRLRLPCGASVEGLWILGNLVGNSKFSFAPDSEWNDPSY
Sbjct:    1 MIVKYEGAIYHGTMEGQGRAEYDDGEVYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXRQTFASGGGGGGRGDEPEKVAATGGENEWYDGQWKSGAFHGQGEYHFANSGDVYVXXXXXXXXXXXXXXXXXXXXXXXXXXLWCDLRHGHGVHSGPDSTYDGXXXXXXXXXXXKLSDKDGNVFEGFFREXXXXXXXXXXXXXXXXXXXXXXXXXXERGRRSGEGTMFAAKTXXXXXXXXXXXXXXXXXRLRLPCGASVEGLWILGNLVGNSKFSFAPDSEWNDPSY 359
[Close]
BLAST of mRNA_Ecto-sp6_S_contig98.18776.1 vs. uniprot
Match: D7G0U9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0U9_ECTSI)

HSP 1 Score: 56.6 bits (135), Expect = 1.280e-5
Identity = 26/36 (72.22%), Postives = 28/36 (77.78%), Query Frame = 1 
Query:  403 VAATGGENEWYDGQWKAGSFHGQGEYHFADSGDVYV 510
             AATGGENEWYD QWKAG+ HGQG   FA SGD+ V
Sbjct:   41 AAATGGENEWYDKQWKAGACHGQGGIRFAGSGDMCV 76
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp6_S_contig98.18776.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D7G0U8_ECTSI2.500e-8181.62Phosphatidylinositol-4-phosphate 5-kinase n=1 Tax=... [more]
D7G0U9_ECTSI1.280e-572.22Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp6_S_contig98contigEcto-sp6_S_contig98:343320..348531 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species6 EcLAC_371 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score266.2
Seed ortholog evalue2.2e-68
Seed eggNOG ortholog2880.D7G0U8
Model size1641
Hectar predicted targeting categoryother localisation
Exons9
EggNOG free text desc.Translation initiation factor IF-2, N-terminal region
EggNOG OGsCOG5253@1,KOG0229@2759
Ec32 ortholog descriptionMORN motif
Ec32 orthologEc-23_002230.1
Cds size1026
COG Functional cat.T
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp6_S_contig98.18776.1prot_Ecto-sp6_S_contig98.18776.1Ectocarpus species6 EcLAC_371polypeptideEcto-sp6_S_contig98 343320..347916 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682350605.0336454-CDS-Ecto-sp6_S_contig98:343319..3434011682350605.0336454-CDS-Ecto-sp6_S_contig98:343319..343401Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 343320..343401 +
1682350605.0457637-CDS-Ecto-sp6_S_contig98:344019..3440511682350605.0457637-CDS-Ecto-sp6_S_contig98:344019..344051Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 344020..344051 +
1682350605.056222-CDS-Ecto-sp6_S_contig98:344778..3448481682350605.056222-CDS-Ecto-sp6_S_contig98:344778..344848Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 344779..344848 +
1682350605.0670464-CDS-Ecto-sp6_S_contig98:345644..3457331682350605.0670464-CDS-Ecto-sp6_S_contig98:345644..345733Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 345645..345733 +
1682350605.0773277-CDS-Ecto-sp6_S_contig98:345877..3460761682350605.0773277-CDS-Ecto-sp6_S_contig98:345877..346076Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 345878..346076 +
1682350605.0877025-CDS-Ecto-sp6_S_contig98:346383..3464941682350605.0877025-CDS-Ecto-sp6_S_contig98:346383..346494Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 346384..346494 +
1682350605.0976622-CDS-Ecto-sp6_S_contig98:346653..3468031682350605.0976622-CDS-Ecto-sp6_S_contig98:346653..346803Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 346654..346803 +
1682350605.108706-CDS-Ecto-sp6_S_contig98:347416..3475431682350605.108706-CDS-Ecto-sp6_S_contig98:347416..347543Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 347417..347543 +
1682350605.118895-CDS-Ecto-sp6_S_contig98:347750..3479161682350605.118895-CDS-Ecto-sp6_S_contig98:347750..347916Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig98 347751..347916 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682350605.1291037-UTR-Ecto-sp6_S_contig98:347916..3485311682350605.1291037-UTR-Ecto-sp6_S_contig98:347916..348531Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig98 347917..348531 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp6_S_contig98.18776.1

>prot_Ecto-sp6_S_contig98.18776.1 ID=prot_Ecto-sp6_S_contig98.18776.1|Name=mRNA_Ecto-sp6_S_contig98.18776.1|organism=Ectocarpus species6 EcLAC_371|type=polypeptide|length=342bp
MIVKYEGAIHCGTMEGQGRAEYDDGEARMWKNGMRHGTGTLTFTNGSTYS
GSWDKDARQGQDGQACGAGTQTFGNGDRYDGDWKDGMMSGKGTYVSANGD
RYEGEWTDDEKNGKGRQTFASGGGGGGRGDEPEKVAATGGENEWYDGQWK
AGSFHGQGEYHFADSGDVYVGSWVGNQMHGKGKIVYGGDGGEYQGDLWCD
LRHGHGIHSGPDSTYDGAWSSDRRHGHGKLSDKDGNVFEGFFREGGWDNG
QLTGHATVQYKNGDRYTGGFEKGRRSGEGTMFAAKTGYEFTGTFEKGVKN
GSGRLRLPCGASVEGLWILGNLVGNSKFSFAPDSEWNDPSY*
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mRNA from alignment at Ecto-sp6_S_contig98:343320..348531+

Legend: polypeptideCDSUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp6_S_contig98.18776.1 ID=mRNA_Ecto-sp6_S_contig98.18776.1|Name=mRNA_Ecto-sp6_S_contig98.18776.1|organism=Ectocarpus species6 EcLAC_371|type=mRNA|length=5212bp|location=Sequence derived from alignment at Ecto-sp6_S_contig98:343320..348531+ (Ectocarpus species6 EcLAC_371)
ATGATCGTCAAATACGAAGGAGCCATCCACTGCGGCACCATGGAAGGCCA
AGGGCGGGCCGAGTACGACGACGGCGAGGCAAGTTTCCTCACCGCTCTTC
GCGAGTGATCTGCCGTCAACTACTGAAACGGGTGTTTCATTCCTAGAGTG
GGACAGTCAAGAGGAGAATGGTTGCTGGTCTCTCCGAGCTAACCAAGCAA
GAGCACTGACCATGTGCCCTGGTTATCACCGAATGAACGGTACTCAATAG
AGGATACTGTCGTGATTGATATTCCTTTGTGGAAAAACTTACCTCTTCAA
GAAACGATGGGCTCAGACTCTGTTGGCTCAGCCGTAGGAGCGGCCGATGC
ACCCCCTTTGCTATTTTGAGCATATTTTTTCCACGTGATGCACTTCACTG
CTTGGATCCGTGGTGCTTCATCCCTGGATTAAGGTAGGTGCTCGCCAATG
GATATTCACTGCATTTAGTTATCGTTGTGCGTTCGGGCTGGCTTGGTCAC
AGCTGTGGTTTTCCTGAACAATGCGGCCGCACTTGCAGCGAACAGCATGT
ACTGCAATCATCGTCCGAAACCTCGGATTTTCTCACCCTACCGGGGTGCT
GTTCCACGCCGCCCCGTTCAAGTTTCCCCTCCTCTCCTCTTCCCTGATCC
CTCTACGCTGCCTTGCTTCTTGGATCCTATCTCGCATCAGGCGTATGAAG
GAATGTGGAAGAATGGCATGCGTCATGGCACGGTGAGATGGGAGAAATGT
CCAGCACTCACAGGAGTGTGCGTCTCTGCTGTGATGTAGAAATACACTGA
TCTTTGCTTCGTAGGTCATCATCTCGTCTCACTTCGAGTTAAACGCGATA
CTCGTGCTACGGACATGGCTACAAACAGTCAAGCAAGAGCCAGTCGGCTT
CTACTGTAGTCAGGTAGTCCTTGAACGTTGGCTGGGCATGCTGCTGCCAT
CACTATATGCTGGGCCGAAATGAAATCATAAGCAACAGACCCATGCAATT
CTTCAAATCACGTTCTGTCCGCGGAGCTGCCAGCAAGTATTGCACACGCT
GGCTCTCGGAGTTCAAAGATTTGGTTCTACTCACCGCAGGCGTATCGAGG
TCTTGCGAATGACTTCAATGAAAATGGAACTTTCTACTCACTTCTTTCCG
TCCCATTCGTCTTGGATTGCTGTGGATGACCAACATCTGAGAATGGGCGA
TAACCTGGCATATTTTGTTCAAATGTGATTGATTCATCCACCAGTGTCTG
GAGCAACGGACAGTATACGTCGTGTAACCCGTTTCCGTGAAGCTTTCATT
TCCAGCCGAGACGTTTCTGGCGACATCGATTAACCACCTATCCCAGTAAA
GTCACAGTTGGGCCAACGTCGCTCACGCGTCTCACGATCGCCAAACCGTC
AACCATTTCCTGATGTAATGAGGCAACCCGTACTTCACTCCCGACTAATT
TGTTGGAAGGGTACACTCACGTTCACGAACGGTTCGACCTACAGCGGCTC
CTGGGATAAAGATGCTAGACAAGGCCAAGGTATGCCGTGGAAATGTTGGG
ATATGAGGATGTACACTTCATTCCGCAATGTCGAGGGCATTTCAGGGAGA
CCCATTGGCGTTTGAGTCGAAATGAAAGGTGCTCCGAATGGAAGTGAAAT
GGAAGCCTCGAAGCCAGAGGATGGGTCGTGGCGGGGTGATTCTTGCCAAA
TGGGATAAGTATTTCATTTCTGACCTCGACAACATTTGGTGCATGTGGAC
TCTCTGTGGGCATAAAAAGCAAGGCTTTTGACTACACTCAAATCTTCAGT
TGTGATCCAGATGAAGATGGTCCACATCTAGTGTATCAATGGTTGAGGGA
TGATGCCACGCATTAAATACGGTTGAACTCGACGTTGCTATCGGTCTCCC
TGACGTTCATACCCACACGGCGAAAAGAGGTATCGAGATCACGGACCGGC
GCATCCGTACATTCCCACCACAAAGCCGGCCTATCGTGGCCACGTGAGAG
TCGTTCGCTTTCGAATCGAGACAACCACGAATCATACCGACCCTCTCCAG
GTTGGCGTATGCTGCGGGTGATTGTGGTACTCCTCAATCCGCCCATTAGT
GAATATTCCTCAGGACCCGGTATTCGCAGGGGACGCGTTCTCAGCACATG
GCTCAAACGCGCGCTTTTCTCGCTGACGACCAAGTCTACAGATGGAATTC
CTGCATGCCTTTTGTGAAATTTCCCGAGGGAGTGAATCGATCAAGAATCG
CGTGAAAGTGTTTGTGCATGCCTCCAGGAACATGCCACTACACCAACGGT
AATACCTACACGGGTTCATGGAAAGATGGACAAGCGTGTGGTGCCGGGAC
ACAGACCTTCGGTAACGGTGATCGCTACGACGGAGATTGGAAAGATGGAA
TGATGAGCGGCAAGGTAGGTTCCGCCCGCATGCTACTTGACGTATGCAGC
AGTGAGTTTTCAAAGGCTTCATGCCGCCGTAGCTTCAGACGGTTCCCCTG
AAGATCCAATGCCGCTCGCCGAGTCGGATTCGGCGGTCCGCTGGGTGATG
TCCACCAGGGTACCTACGTGAGTGCGAATGGAGATAGGTACGAAGGGGAG
TGGACGGACGATGAGAAGAACGGCAAGGGGCGTCAGACATTTGCCAGCGG
TGGCGGGGGCGGCGGCAGAGGGGATGAACCCGAGAAGGTGGCAGCTACCG
GTGGTGAGAACGAGTGGTACGACGGGCAGTGGAAAGCTGGATCATTTCAC
GGTCAAGGTGCGCTTTCCTCCTACTAACGCCAACAGAGGAAGCAAGCATG
AAAAAATTCTGTAGTCACTGTGTGAAACATATCTGACCCTTGTCCACACG
TGGATGGCTTTCCGGCCCACTGCAGACAACAAGTAGATGACTACGCGTCG
TCTACAAAACGTTTTCCTATGGGTCCATTCACCCCCGTAACATATGAATT
ACCGCTTGGGAACACAATGCTGCCCTGGTGCGATCTCCGCACACTATCGT
GGCGGTCAATGCGAAATTTTTAGCTGATATATTTGCCTGCCCCAAGCGCA
CGTTTGGTACACAGGAGAATACCACTTCGCCGATAGCGGCGACGTGTATG
TCGGCTCCTGGGTGGGGAACCAAATGCACGGGAAGGGGAAGATCGTGTAT
GGCGGTGACGGTGGAGAGTACCAAGGTGGGCCTGCGTCCCAACTACAGAG
CGTTGCATAGAGCAGGGCATCTCACACCACCAACTGAACCCGAGATCAAG
TGAATGGTTGAGTGAATCGTCTGAATGTGGGGGCATCCTATCATTCGCAT
GGGGTATTGCTGACGAACCGCCCATACTCGACAGGTGACCTATGGTGTGA
TCTTCGACACGGTCACGGCATACACAGTGGCCCCGACTCTACCTATGACG
GTGCCTGGAGCAGCGACCGCAGGCACGGCCATGGAAAGCTCAGTGACAAG
GACGGGAACGTGTTCGAGGGTTTCTTCAGAGAAGGTTTGCACCGGAATCA
ACATTTGAACTGGGTATTCCGCGCGTAGAACACGAGACACTGATACTGGA
GGTAGTTGAGGATTTTGTGGGTATTAGTGCAGCTGCCGGTGGCGACACGA
TGATAGCAATCATCCCTTGTCGCGCATTGACTGCGGTACCGTAATGCTTC
ACCGCCAGCATCACCGTTCGGTGATTTTTCACGTCCCTCTCCACGCAGGA
GAAAAGCATGGCAACGGTCAACTCAAGTCCGCGGCAGGCGACGAGATTGA
AGGTGGCGACAAGGCCGTTCTCGTCTGTATTCGAAGGGCCTCCGTTTAAC
GTGCCTTCGTAGCATTCATGAGATTTTTGTCATTGCTGCCTCACGACAGA
GTGAAAAACTGCGTTGGTGGCGATGATGAGTATCTTGGCCTTATTCACAA
AAATCGCACGTGGTACCTGTGGTTGACGGTGCTCGGTCTTTGGGCATGGA
CTTGGTTACACAGGGACGGTATCGAAAACTAAGATTTTCCCAACTGGCCA
GGCGGTGAAAATTCCTCTGATGCTTGTTCTACCTTCCTGGTACCTTCCTG
GTATTACGGAACGCATAACACTTCCGTGAATTGTGTCGAGTGGAAAGGTG
GGTGGGACAACGGGCAGCTCACCGGTCATGCGACGGTTCAGTACAAGAAC
GGTGATCGGTACACCGGCGGCTTCGAAAAGGGCCGGAGGAGCGGCGAGGG
AACTATGTTCGCCGCAAAAACGGGGTGAGGCAACACTGGAGAATCGTCAT
TGTTGAAATGCATGGGATTGTTCAGTACGACCTCTGGAATGAATCTCCGC
GATGTTGTGGTTCTGGGAGTAAAGGCGCTGTCGCCGTCTGGGGGGTTATT
GAGGATTGTCATCTTCAGATCGAAACACCCTCGAACCGACCGAAAGCTCT
CGCTGCCGTTGCTCTCTTGAAATCGTGAAAGCTACGAGTTCACCGGCACC
TTCGAGAAGGGCGTGAAGAACGGTTCGGGACGTTTGCGCCTTCCGTGCGG
CGCGAGCGTAGAAGGCCTGTGGATATTGGGCAATCTCGTCGGGAACTCGA
AATTTTCGTTTGCACCTGACTCCGAGTGGAACGACCCGAGTTACTGATGC
TTGGCGATCCTTTTGCGTTCATGGTCTCGACAATCACGGAGGTCTGCGTA
GTCGCGCCGTCGCGTTTCCTTGGCAAGAAGACTGCGCCGATGCGTTGTCC
CGAAAATGCAGTGCCGAAAATGCAATGAGTCAACCAAGTTTGGGCCGGTA
CATTACCCTAGGTGATTCCGTAGTGACAATCATTTTTTGCCATCGATAAA
GTCTCTACGTAGGTGCGGAGGGTTTCGTTGATGAAGATGTATCGCCCGGA
GTCGGACATGGTGATTCCCCCGGGGCTCCCATTCATTCCTCTTTTTTTAT
GTGCGCCCTCCATTTCTACTCAAGCAAGTACTTTTAAAATTAGCCATCCC
CCCTCGCATGTTCGAGTTGACGGGCTGTTGTTTTCACGAAGAGGGGGGCA
GTGATCAGGATCTGTGAACCCCGTATGGCAATGTCAAGGTGTTGTCAAAG
CACGATCATCAGTAGAGCGCGTGCTCTCAAGTGGGACAAGTGCTAAGGGA
ACGAGGTCATCAGCTTGTGAGATGTCTGTAGGGGGGGCGTGCCCTTTACG
TTTTACATGCCTTAGCGGTTAGACCATTCGCCACGCGTCCTCAAGATGAC
GGAGCATTGTTC
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Coding sequence (CDS) from alignment at Ecto-sp6_S_contig98:343320..348531+

>mRNA_Ecto-sp6_S_contig98.18776.1 ID=mRNA_Ecto-sp6_S_contig98.18776.1|Name=mRNA_Ecto-sp6_S_contig98.18776.1|organism=Ectocarpus species6 EcLAC_371|type=CDS|length=1026bp|location=Sequence derived from alignment at Ecto-sp6_S_contig98:343320..348531+ (Ectocarpus species6 EcLAC_371)
ATGATCGTCAAATACGAAGGAGCCATCCACTGCGGCACCATGGAAGGCCA
AGGGCGGGCCGAGTACGACGACGGCGAGGCAAGAATGTGGAAGAATGGCA
TGCGTCATGGCACGGGTACACTCACGTTCACGAACGGTTCGACCTACAGC
GGCTCCTGGGATAAAGATGCTAGACAAGGCCAAGATGGACAAGCGTGTGG
TGCCGGGACACAGACCTTCGGTAACGGTGATCGCTACGACGGAGATTGGA
AAGATGGAATGATGAGCGGCAAGGGTACCTACGTGAGTGCGAATGGAGAT
AGGTACGAAGGGGAGTGGACGGACGATGAGAAGAACGGCAAGGGGCGTCA
GACATTTGCCAGCGGTGGCGGGGGCGGCGGCAGAGGGGATGAACCCGAGA
AGGTGGCAGCTACCGGTGGTGAGAACGAGTGGTACGACGGGCAGTGGAAA
GCTGGATCATTTCACGGTCAAGGAGAATACCACTTCGCCGATAGCGGCGA
CGTGTATGTCGGCTCCTGGGTGGGGAACCAAATGCACGGGAAGGGGAAGA
TCGTGTATGGCGGTGACGGTGGAGAGTACCAAGGTGACCTATGGTGTGAT
CTTCGACACGGTCACGGCATACACAGTGGCCCCGACTCTACCTATGACGG
TGCCTGGAGCAGCGACCGCAGGCACGGCCATGGAAAGCTCAGTGACAAGG
ACGGGAACGTGTTCGAGGGTTTCTTCAGAGAAGGTGGGTGGGACAACGGG
CAGCTCACCGGTCATGCGACGGTTCAGTACAAGAACGGTGATCGGTACAC
CGGCGGCTTCGAAAAGGGCCGGAGGAGCGGCGAGGGAACTATGTTCGCCG
CAAAAACGGGCTACGAGTTCACCGGCACCTTCGAGAAGGGCGTGAAGAAC
GGTTCGGGACGTTTGCGCCTTCCGTGCGGCGCGAGCGTAGAAGGCCTGTG
GATATTGGGCAATCTCGTCGGGAACTCGAAATTTTCGTTTGCACCTGACT
CCGAGTGGAACGACCCGAGTTACTGA
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