mRNA_Ecto-sp6_S_contig95.18568.1 (mRNA) Ectocarpus species6 EcLAC_371

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 1830 bits (4740), Expect = 0.000e+0
Identity = 935/979 (95.51%), Postives = 951/979 (97.14%), Query Frame = 1

Query:    1 MGLRADHVAAILLVASPSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHGPDTSPYVGATDDFNLSDLSAPLRSTYGEGMTLDNYDVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLLPGKKASSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 2937
            MGLRADHVAAILLVASPSAV+GVDLGKIQTITASSAFDDFARSQSAYSAAS+WHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLET FT+SAVHR+SNTETDGSCFIVTALPSSATAMI APEDFALLSAAP LPSLKLAT LLDHGPDTSP+VGA DDFNLSDLSAPLRSTYGE  +   YDVTRV EWSRAAAVVDSLASKRAQTFGE+CGFGKL LRHVADDLLLV+GMNHLLPGKKA SEARTACYMGLLSQLAAKPEVLR+SPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQ+EHGFLF GVR EGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQ+SFTYASDYIRDYLGD FDDDDSEYNSYD      SSDTWSEESFCAQ LDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSL+EEACYGDELPGCAGGCISASDVD MLDNGSFDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYD LCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCT+GSPALGKNILAVGATSSGPSRGTRTG+DGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELV VDGDAGAELRATLAWLDPPTTALSAKQLQHDLDL+VIAPSGAKYTMWESGADDTVNVVERVIVPVE MESG+WSVVVSAKGLLTDEQSYSLVVTGAIYSSK
Sbjct:    1 MGLRADHVAAILLVASPSAVIGVDLGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGEDRS--QYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 977          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1607 bits (4160), Expect = 0.000e+0
Identity = 843/1024 (82.32%), Postives = 885/1024 (86.43%), Query Frame = 1

Query:   34 LLVASPSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASEWHTKRLAH-APEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHGPDTSPYVGATDDFNLSDLSAPLRSTYGEGMTLDN-------------------------------------------------------YDVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLLPGKKASSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 2937
            +LVAS S VLGVDLGKIQ ITASSAFDDFARSQSAYSAAS W+  RL++ A EGS APY+ACADYKQGRHA+TSLET FTKSAVHR+SNTE DG+CFIVTALPSSATA+IRAPEDF LLSAAPLLPSLKLATSLLDHGPDTSP +GA DDF+LSD SAPLRSTYGEGMTLDN                                                       YDVTRV EWSRAAAVVD LASK A+T GE+CGFGKL+LRHVADDL+LV+GMNHLLPGK+  SE RTACYMGLLSQLAAKPEVLR+SPLQSA L+NAVANALVQSATTTETPLLDAGLDGTGEVIQVVD+GLDETSCFFADDDGLQ+EHGFLFDGVR E DGNVSTVLGNYSFPFDMSRRKIVQYIQ+FKTPDQGSTSSGQDSFTY SDY    LGDP+D DDSEY SYD       SDTWSE+SFCA  LDDLSYHFS LFDDTSYS SS S S   TGGF KD VGGHGTWTAG AAGAISAQS LEEEACYGDELPGCAGGCISASDVD MLD+ +FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIS+ D SY G++ +A FAGN LWYSAMETGAKIH+NSWG  TLCQLTE+EYLYD+FMYENPEHLLIFAAGN GG EDIPN E CTVGSPALGKN LAVGATSSGP  GTRTGDDGNLVYEE G+TDYSPEGYPWICL+P LGTPSAS EPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSC+L+A+MGTSASCPLVAGAAALVRQYFKDPSFFAKDV SRGGCAEHSSS QCSEFAPSAATVKAMIINSAHLMGGSSEPNG+RGFGRVHLEAGMPMDGVGETGL VVEA I +D EITELV VDGDAG ELRATLAWLDPPTTALSAKQLQHDLDL+VIAPSGAKYTMWESG DDTVNVVERVIVPVE MESG+WSVVVSAK LLTDEQ YSLVVTGAIYSSK
Sbjct:    1 MLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDY----LGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSF--TGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAI-ADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1017          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 916 bits (2367), Expect = 0.000e+0
Identity = 495/717 (69.04%), Postives = 547/717 (76.29%), Query Frame = 1

Query:  325 VTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHGPDTSPYVGATDDFNLSDLSAPLRSTYGEGMTL-------------DNYDVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLLPGKKASSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTE---TPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDGHSCRL 2277
            V ALPSSATA+IRAPEDF LLS  PLL SLKLATSLLDHGPDTSP +GA D+ +LSD SAPLRSTYGEG+ +               YDVTRV EWSRAAAVVD LASK A+T GE+CGFGKL+LRHVADDL+LV+GM +LLPGK+  SE RTACYMGLLSQLAAKPEVL +SPLQSA+L+NAVANA +QSAT  +    P    G           D+GLD+TSCFFADDDGLQ+EHG+LFDGVR E DGNVST LGNYSFPFDMSRRK                                                 IVQ IQ+FKTP QGSTSSGQDSFT+ SD+I    GDPFD DDSEY SY        SDTWSE+SFCA  LDDLSYH S L+DDTSY+  S S+S S TGGF +D +GGHGT T+G AAGAIS+QSSL+EEACYGDELPGCAGGCISASDVD MLD+ +F L VFCPMYTCDGN DIPSSECLSDDPVE+LHQHGGVAPGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG  TLCQLTE EY+YD+FMYENPEHLLIF+AGN GG +DIPN E CTVGSPALGKNILAVGATSSGP  GTRTGDDGNL  EE G+TDYSPEGYPWICL P LG P AS EPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTD HSC+L
Sbjct:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEGVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFI----GDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYA--SPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 620 bits (1598), Expect = 1.030e-201
Identity = 417/1017 (41.00%), Postives = 552/1017 (54.28%), Query Frame = 1

Query:   97 ASSAFDDFARSQSAYSAASEWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHG-------------------------------------PDTSPYVGATDDFNLSDL-------SAPLRST-------YGEGMTLDNYD-VTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLL-PGKKA-------SSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVST----VLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSF-----DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFA--GNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEA--TIGSDDEITE-LVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGAI 2925
            A++A DDF     A+ AA+ W+ +RL  + +G+RAP++ CA+Y  GR   + L+   +  AV  +S++E  G+CF+ TA  + A+A++   E F L S +P   +LKLA  L+DH                                      PDT P   A  +  + DL       S  L ST          G  L ++    R  +WS+AA+VV  L+     + G++C + ++ L    DD LLV G++HLL  G+ A       S+E   AC+MGL+S  A +PEVLR+SP  +  + N  A  ++QSA+ T+TPL DAGLDGTGEVIQVVDTGLDETSCFF DD G +++HG+ F+ +     G+       V     F +   RRK++QYI++   PD              SDY+                                      F       +  L  D                 F +D   GHGT TAG AAGA +     E   C GDE  GC GGCI  S+    L + +      DLD  CPMY CDG+TD     CLSD+  E L ++GG+A GA++S+ D  +   + F L    GN +W   +E G K+HS S G DTLC   E+E LYD FMYENPE+LLIFAAGN G  E       CT+ SPA+GKN+LAVGA++SG +R         ++  E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSCRL A  GTS SCP+VAGAAA+VRQYF D +F+  DVT+RG C++      C  F+PSAATVKA++INSA+LMG SS+P+  RGFGR+HLE GMP++G G+  L V +A  T  ++D + E L +VD DAG ELRATL+W+D P T+ SA QL +DLDL V++PSG  Y MW S   D  NV ERVIV  + +E+G W+V+V +  L+TD QSYSLVV GAI
Sbjct:   42 ATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVN-PD--------------SDYVA-------------------------------------FSTTQGGVYPFLPPDE----------------FGQDDSAGHGTHTAGSAAGA-TLNDPAETLTCSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSGDTR---------VISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 570 bits (1469), Expect = 3.770e-189
Identity = 312/495 (63.03%), Postives = 338/495 (68.28%), Query Frame = 1

Query: 1054 YSFPFDMSRRKIVQYIQMF---KTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDT-------------------------------------------------W-----------------------------------SEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRL 2277
            +S P+  +   +V Y  +    KTPDQGSTSSGQDSFTY SDY     GDP+D DDSEY S+D       SDT                                                 W                                   SEE      LDDL+YH S L+DDTSY+ SS S S   TGGF KD +GGHGT TAG AAGAIS+QSSLEEEACYGDELP CAGGCISASDV+ MLD+ +FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGG APGAQIS+ D SY G++ +A +AGN LWYSAMETGAKIH+NSWG  TLCQLTE EY+YD FMYENPEHLLIF+AGN GG EDIPN E CTVGSPALGKN LAVGATSSGP  GTRTGDDGNL YEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L
Sbjct:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYF----GDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSF--TGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 575 bits (1482), Expect = 7.610e-185
Identity = 397/1064 (37.31%), Postives = 533/1064 (50.09%), Query Frame = 1

Query:   82 IQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHGPDTSPYVGATDDFNLSDLS-------APLRSTYGEGMTLD----------------------------------NY-----------------DVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLL-PGKKA-------SSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASD-----VDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEA---TIGSDDEITELVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGAIYSSK*RMTPPCWSPSTGQLSVRFRVLRGENTALLATVVVGVG 3051
            ++    ++A DDF     A+ AA+ W+ +RL H+ E +RAP++ACA Y  G  A + L+   +  AV  +S++E  G+CF  +A  + A A++   E F L + +P    LKLA  L+DH   T  +  A ++   S L        A +R    EG++++                                  N+                    R  +W +AA+VV  L+   +   G++C + ++ + H  DD LLV G++HLL  G+ A       S+E   AC+MGL+S LA +PEVLR+SP  +  + NA A  ++QSA+ T+TPL DAGLDGTGE+IQV+D+GLDETSC+F DD G +++HG+ FD +     G  +    +YS                    D+ +T      F+Y                                                                           + +    GHGT TAG AAGA +     E   C   +   C G CI   D     V         DLD  CPMY CD +TD   + CLSDD  E L  +GG+A GA++++ D  Y G D+ +   GN LW    E G K+HSNS G D  C +   + L D FMYEN E+LLIFAAGN GG E       CT+ +P +GKN LA+GA++SG +R          V    G T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSCRL A  GTS SCP+VAGAAA++RQYF D +F+  DVT+RG C++      C  F+PSAATVKA++INSA+LMGGSSEP+G RGFGRVHLEAGMP++G G+  L V +A   +I        L +VD DAG +LRATL+W+DPP TA SA QL H+LDL V++P G  Y MW S   D  NV ERVIV    + +G W+V V A  L+TD QSYSLVV GAI            SP TG+ +         ++A  A+   G G
Sbjct:   32 VENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDH---TESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDEL-----GRAAVYSSSYS-------------------SDETATIFNGGDFSY---------------------------------------------------------------------------YPERRKAGHGTHTAGSAAGA-TLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGDTR----------VISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSD---GFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI------------SPGTGEGASGSSSFEVSSSATSASSEDGTG 933          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 572 bits (1473), Expect = 2.730e-182
Identity = 409/1054 (38.80%), Postives = 552/1054 (52.37%), Query Frame = 1

Query:   82 IQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHG-------------------------------------PDT----SPYVGATDDFNLSDL---SAPLRSTY---------GEGMTLDNYDVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLL-PGKKA--------SSEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFA-DDDGLQIEHGFLFD--GV-----------------------RAEGD---GNVSTVLGNYS---FPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCIS---ASDVDTMLDNGS--FDLDVFCPMYTCDGNTDIPSSE-CLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFAL--FAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGT-DGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITEL---VNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGAI 2925
            ++   +++A DDF     A+ AA+ W+ +RL H  + +RAP++ CA+Y  GR   + L+   +  AV  +S++E  G+CF  +A  + A+A+I   E F L S +P   +LKLA  L+DH                                      P T    S    A     L DL   S  L ST          GE ++     V R  +WS+AA+VV  L+     + G++C + ++ + H   D+LLV G++HLL  G+ A        S+E   AC+MGL+S LA+KPEVLR++P QS +L NA A A +QSAT T+TPL DAGLDGTG+V+QV+D+G+DE+SCFFA D+ G  + HG  F+  G+                       RA  D   G+   +   +    F     RRKI+QYI + K+ D  +  S  DSFT                                                     +SL     + P+           F+ D   GHGT  AG  AGA +  +  E  +C   E   C G C+    A+DVD +        DLD  CP+++ D + D+  +E CLSDD  E L  +GG+A GA++++ DA Y   D+F L  F GN LW    E   KIHSNS+G D  C L  N+ LYD +M  NPE+LLIFAAGN G          CT+ SPA+ KN+LAVGA++SG +R                           +  +P LG          +DTVA FSS GPT D RIKP+V+APGD + SA+SDG+ D HSCRL A  GTS S P+VAGA+A++RQYF + +FFA D+T+RG C E +    C  F+PS+AT+KA++INSA+LMGGSSEP+G RGFGR+HLEAGMP+ G G   L V ++   S  E T      +V+  A  +LR TL+WLDPP T  SA QL HDLDL VI+P+G +YTMW SG  D+ NV ERVIV    +E+G WSV + A  L TD QSYSLVV GAI
Sbjct:   27 VENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAV-RARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDD--TEGSHGDSFT-----------------------------------------------------TSLGQQYPWLPADR---------FEYDYEAGHGTHVAGSVAGA-TLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFY---DNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR----RSTCTMSSPAIAKNVLAVGASTSGDTR---------------------------LSATPGLG----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG---CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 545 bits (1404), Expect = 1.870e-174
Identity = 379/1006 (37.67%), Postives = 507/1006 (50.40%), Query Frame = 1

Query:   49 PSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPE-GSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHG--------------------------------------PDTSPYVGATDDFNLSDLSAPLRSTYGEGMTLDNY-----------DVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLLPGKKAS-------SEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISAS------DVDTMLDNGS---FDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITE---LVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWE-SGADDTVNVVERVIVPVEPMESGMWS 2856
            P   L V  G + T TA  +FD  A+   A+  A++W  + L    E  +RAP++ C +Y +G   +  L+ + +  AV  ++++ T G+CF VTA  + A AM  +P  + L S +P   +LK+A  +L+HG                                      P  SP  G      L DL +     +G  +  D                R  EWS AA +V  LA     + G++C +  + + +  +DLLLV G++HLL  +  +       +E R AC+MGL+S L+ + EVLR+SP  +  L NAVA  ++QSAT TE PL DAGLDGTG+VIQV+DTGLDETSCFF DDDG ++ HG  ++ +                        +I+                                                                                     PSS+S+         +D V GHGT  AG A GA     +     C G E+PGC GGCI A       D+ T++   S    D+D  CPM  CD  TD     CLSDD  + L  HGG+A GA+++  D    GS S   F GN LW   +E G KIHS S G D  C + E + LYD FMY NPE+L++ AAGN G  ED      CT+ SPA+GKN LAVGATSSG +R T+T  DG+L+    G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSCRL A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG   +  S  +C  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   L V +++  S  E+     + +VD +A  + RATL+W+DPP ++ S  QL HDLDL V++PSGA + MW  SG  D+ NV ERVI+  E +ESG ++
Sbjct:   30 PYPSLSVVEGSLLT-TAQDSFDGHAK---AFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPY-YGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG-----------------------EII-------------------------------------------------------------------------------------PSSSSA---------RDDVEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGS-SGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDD--SGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 546 bits (1406), Expect = 3.200e-173
Identity = 387/1053 (36.75%), Postives = 533/1053 (50.62%), Query Frame = 1

Query:   31 ILLVASPSAVLGV-DLGKIQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPEGSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHGPDTSPYVGATDDFNLSDLSAP-LRSTYGEGM-----------------------------------------TLDNYDVT--------------------RVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLL-PGKKAS-------SEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRA---------EGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISASDVDTMLDNGSFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEAT---IGSDDEITELVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWESGADDTVNVVERVIVPVEPME----SGMWSVVVSAKGLLT-DEQSYSLVVTGAI 2925
            +LL+++ S    V  LG  +    ++A  DF  S++A++ AS W         +G  +PY++C ++ +GRHA   LE      AV  +S+T T G+CF+VTA P+ A A + + EDF    A P   +LK+A  LLDHG    P          SD SA  L +T+G  M                                         +LD + +T                    R  EWSRAA +V+  +    +T  +VCG+ ++ + H A+D+LLV G++HLL  G++ S       +E R ACYMGL+S LA++ EVL+++        NA A A +QSA+   TPL DAGLDG+GEVIQ++D G++ET+CF    DG ++EHG+ FD V +         E             F +D  RRK++QYI +   PD G  SS    F  A  ++ D                                      DDL                                  GHGT  A   AG+  +  +       G E+ GCAGGCI+ S   +   +   D++  CP + C G+      +CL DD  + L +HGG+A GA+I+ +D  Y G  S+   AGN LW +  + G KIHSNS+G D  CQLT  + LYD +MYEN EHLL+F+AGN G   D    + CT+ SP + KN+L VG+TSSG +R                 TD    GY            +   E + +D V+ FS  G T DGRIKP+VVAPGD + SAS +G D  SC L+A  GTS S  + +GAAA+VRQYF+D SF++ DV SRG C +  +   C  F+P A TVKAM+INSA++MGG +EP+  RGFGR+HLEAGMP+ G G+  L V +++   + SD+++    NVD DAG ELRATL+W DP  ++LS  QL +DLDL V AP G +Y MW+    DT NV ERVIVP E +     +  W V V  +     D  S+SLVVTGAI
Sbjct:   12 VLLISTVSGASQVPSLGFFEQTLLTTAGADF-DSEAAFAFASAWQAGDR----QGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEA-AQLSSWEDFTTFGAFPA--ALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD--------------------------------------DDL----------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSGVTR--------------LSTTDLDGRGY------------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGD-SSCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFT---CDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 943          

BLAST of mRNA_Ecto-sp6_S_contig95.18568.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 526 bits (1355), Expect = 1.780e-166
Identity = 382/1038 (36.80%), Postives = 510/1038 (49.13%), Query Frame = 1

Query:   49 PSAVLGVDLGKIQTITASSAFDDFARSQSAYSAASEWHTKRLAHAPE-GSRAPYIACADYKQGRHAMTSLETVFTKSAVHRISNTETDGSCFIVTALPSSATAMIRAPEDFALLSAAPLLPSLKLATSLLDHG--------------------------------------PDTSPYVGATDDFNLSDLSAPLRSTYGEGMTLDNY-----------DVTRVLEWSRAAAVVDSLASKRAQTFGEVCGFGKLRLRHVADDLLLVQGMNHLLPGKKAS-------SEARTACYMGLLSQLAAKPEVLRISPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQIEHGFLFDGVRAEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKT-----PDQGSTSSGQDSFTYASDYIRDYLGDPFDDDDSEYNSYDXXXXXXSSDTWSEESFCAQFLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLEEEACYGDELPGCAGGCISAS------DVDTML-DNGSF--DLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDTLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTVGSPALGKNILAVGATSSGPSRGTRTGDDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITE---LVNVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLYVIAPSGAKYTMWE-SGADDTVNVVERVIVPVEPMESGMWSVVVSAKGLLTDEQSYSLVVTGA-IYSS 2934
            P   LGV  G + T TA  +FD  A+    +  A++W    +    E  +RAP++ C +Y +G   ++ L+ + +  AV  ++++ T G+CF VTA  + A AM   P  + L S +P   +LK+A  +L+HG                                      P  SP  G      L DL +     +G  +  D                R  EWS AA +V  LA     + G++C +  + + H  +DLLLV G++HLL  +  +       +E R AC+MGL+S L+++ EV R+SP  +  L NAVA  ++QSAT T+ PL  AGLDGTG+VIQV+                                                       QYI + K      P   +TS G   +  + D                                                                       GF  D V  HGT TAG A GA     +     C G E+PGC GGCI A       D+ T++ +N S+  D+D  CPM  CD   +     CLSD   + L  HGG+A GA++S  D  Y G+  F  F GN LW   +E G KIHS S+G D  C + + + LYD FMY NPE+L++ AAGN G  ED      CT+ SPA+GKN LAVGATSSG +R   T  DG+      G +D                          VDTVA FSS GPT DGRIKP+VVAPGD V SA SDG+D HSCRL A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG C +  S  QC  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   L V +++  S  E++    + +VD +A  + RATL+W+DPP ++ S  QL HDLDL V++PSGA + MW  SG  D+ NV ERVI+  E +ESG ++V VS   LLTD Q+YSLVV GA +YSS
Sbjct:   30 PYPSLGVVEGSLLT-TAHESFDGHAKG---FETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPY-YGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-------------------------------------------------------QYINLIKADSVSGPASSTTSGGNRVWWVSPD-----------------------------------------------------------------------GFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSD--------------------------VDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDD--SGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAMVYSS 902