mRNA_Ecto-sp6_S_contig87.17811.1 (mRNA) Ectocarpus species6 EcLAC_371

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp6_S_contig87.17811.1
Unique NamemRNA_Ecto-sp6_S_contig87.17811.1
TypemRNA
OrganismEctocarpus species6 EcLAC_371 (Ectocarpus species6 EcLAC_371)
Homology
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: D7FZB4_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FZB4_ECTSI)

HSP 1 Score: 358 bits (920), Expect = 5.550e-120
Identity = 187/190 (98.42%), Postives = 187/190 (98.42%), Query Frame = 1 
Query:    1 MLRPAVSTLSGRSVAAAVSRSSFPGVCAAGDRLERSRTAGSGGVVGCTRGMMMAAKPGKIKKERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKKDYGLSDDEDADAQLNRFRDGY 570
            MLRPAVSTLSGRSVAAAVSR SFPGVCAAGDRLERSRTAGSGGVVGC RGMMMAAKPGKIKKERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYN KMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKKDYGLSDDEDADAQLNRFRDGY
Sbjct:    1 MLRPAVSTLSGRSVAAAVSRRSFPGVCAAGDRLERSRTAGSGGVVGCRRGMMMAAKPGKIKKERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYNSKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKKDYGLSDDEDADAQLNRFRDGY 190
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A7S1DZY1_HEMAN (Hypothetical protein n=2 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1DZY1_HEMAN)

HSP 1 Score: 63.2 bits (152), Expect = 2.180e-8
Identity = 42/106 (39.62%), Postives = 57/106 (53.77%), Query Frame = 1 
Query:  184 KERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFD 501
            K+   K LD K +   K+L+AR E     S E+   + +I + + R+    H E+   LT KI+LRD AIA LPE LQ   +  D    PL+R + T  PPI GFD
Sbjct:   18 KKGKSKGLDGKFLVHAKVLEARGEERYVRSEEQMGLDTEIAKEFARQSMKRHIEQQKWLTRKIRLRDQAIAALPEELQAEGKSMDEESFPLDRHIWTDFPPIKGFD 123
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A7S1SH10_9CHLO (Hypothetical protein n=1 Tax=Tetraselmis chuii TaxID=63592 RepID=A0A7S1SH10_9CHLO)

HSP 1 Score: 62.4 bits (150), Expect = 1.040e-7
Identity = 39/115 (33.91%), Postives = 62/115 (53.91%), Query Frame = 1 
Query:  181 KKERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKKDYGLSD 525
            K E+   +L  K   ++K+L+ +  P  +LSA + + +   G+ ++RK    H      L+ K++L+  AI  LP  L+  A   D  P PLNR VAT TPPI G+ +   G++D
Sbjct:   61 KDEQQSSSLGGKVQQMMKVLEPQKVPQAKLSAGDLEEHKQRGKEFSRKKMAEHRAWQKALSEKLRLKLAAIEALPVALREHAASDDFEPFPLNRWVATDTPPIEGYYESKSGVND 175
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A445ACG8_ARAHY (Uncharacterized protein n=3 Tax=Arachis TaxID=3817 RepID=A0A445ACG8_ARAHY)

HSP 1 Score: 62.8 bits (151), Expect = 2.010e-7
Identity = 38/93 (40.86%), Postives = 56/93 (60.22%), Query Frame = 1 
Query:  229 LKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKK 507
            L L+D    P  EL+ E+ ++   + + Y+R +   H ER A  TT ++L+ +AI  LPE L+ AA  PD  P P+NR +AT TPPI G+ +K
Sbjct:  138 LGLVDEEKIPKYELTVEDGRK---LAKEYSRVLMRKHRERQAKETTLLRLKKEAIEALPEGLREAALVPDLTPFPVNRFMATLTPPIEGYMEK 227
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A834YQV3_9MAGN (Uncharacterized protein n=1 Tax=Tetracentron sinense TaxID=13715 RepID=A0A834YQV3_9MAGN)

HSP 1 Score: 62.8 bits (151), Expect = 2.220e-7
Identity = 39/93 (41.94%), Postives = 56/93 (60.22%), Query Frame = 1 
Query:  229 LKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKK 507
            L L+DA   P  EL+ E+ +R   + + Y+R +   H  R A  TT ++L+ +AI  LPE L+ AA  PD  P P+NR +AT TPPI G+ +K
Sbjct:  146 LGLVDAEDVPKYELTVEDGRR---LAKEYSRALMRRHRARRAAETTLLRLKKEAIEALPENLKAAALVPDLTPFPVNRFMATLTPPIEGYIEK 235
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A7S2IRI2_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2IRI2_9EUKA)

HSP 1 Score: 60.1 bits (144), Expect = 3.760e-7
Identity = 33/65 (50.77%), Postives = 39/65 (60.00%), Query Frame = 1 
Query:  313 YNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKK 507
            Y+R     H+   A LT  I+LR+ AIA LPE LQH ARQPD    P+ RR+ T T PIP F  K
Sbjct:   80 YSRAKMHEHHRVNAHLTKMIRLREAAIAALPEALQHEARQPDYTTLPIQRRIFTETAPIPDFQLK 144
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A6T7YBG7_9CRYP (Hypothetical protein n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A6T7YBG7_9CRYP)

HSP 1 Score: 59.7 bits (143), Expect = 4.220e-7
Identity = 34/97 (35.05%), Postives = 55/97 (56.70%), Query Frame = 1 
Query:  208 DKKTMTLLKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGF 498
            D +   L+K L+ +        A++ + +  I + + R+     N+ +A++  KI+LRD A+A LP  LQ   R  D  P P++R +AT+TPPIPGF
Sbjct:   38 DSRFKLLIKALEQKGPKKYVRDAKQMEIDQQIAKEFTRQSFRRENKFLANMQQKIRLRDIAVAALPAELQAEGRTNDMEPYPMDRHMATHTPPIPGF 134
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A1S4BHU9_TOBAC (uncharacterized protein LOC107808434 n=12 Tax=Solanaceae TaxID=4070 RepID=A0A1S4BHU9_TOBAC)

HSP 1 Score: 61.6 bits (148), Expect = 4.980e-7
Identity = 37/93 (39.78%), Postives = 56/93 (60.22%), Query Frame = 1 
Query:  229 LKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGFDKK 507
            L L+D    P  E++ E+ +R   + + Y+R +   H +R A  TT ++L+ +AI  LPE L+ AA  PD  P P+NR +AT TPPI G+ +K
Sbjct:  139 LGLVDVEKLPKYEMTVEDGRR---LAKEYSRVLMRKHRQRQAAETTLLRLKKEAIEALPENLKAAALVPDLTPFPVNRFMATLTPPIEGYIEK 228
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A1U8DSZ5_CAPAN (uncharacterized protein LOC107839242 n=4 Tax=Capsicum TaxID=4071 RepID=A0A1U8DSZ5_CAPAN)

HSP 1 Score: 61.2 bits (147), Expect = 6.470e-7
Identity = 37/90 (41.11%), Postives = 54/90 (60.00%), Query Frame = 1 
Query:  229 LKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGF 498
            L L+D    P  EL+ E+ +R   + + Y+R +   H +R A  TT ++L+ +AI  LPE L+ AA  PD  P P+NR +AT TPPI G+
Sbjct:  136 LGLVDVEKLPKYELTVEDGRR---LAKAYSRVLMRKHRQRQAAETTLLRLKKEAIEALPENLKTAALVPDYTPFPVNRFMATLTPPIEGY 222
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BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Match: A0A835LU59_9MAGN (Uncharacterized protein n=1 Tax=Coptis chinensis TaxID=261450 RepID=A0A835LU59_9MAGN)

HSP 1 Score: 61.2 bits (147), Expect = 6.630e-7
Identity = 43/114 (37.72%), Postives = 64/114 (56.14%), Query Frame = 1 
Query:  178 IKKERSGKALDKKTMTL-------LKLLDARPEPMPELSAEEKQRNFDIGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPPPLNRRVATYTPPIPGF 498
            +K +++GK  DK  M         L L+DA   P  EL+ E+ +R   + + Y+R +   H +R A  T  +KL+ +AI  LP+ L+ AA  PD  P P+NR +AT TPPI G+
Sbjct:  115 MKLKKNGKEEDKAIMGTPGLDLISLGLVDAEKIPKYELTVEDGRR---LAKEYSRVLMRQHRKRRAAETNLLKLKKEAIQALPDNLKVAALIPDLTPFPVNRYMATLTPPIDGY 225
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp6_S_contig87.17811.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FZB4_ECTSI5.550e-12098.42Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
A0A7S1DZY1_HEMAN2.180e-839.62Hypothetical protein n=2 Tax=Hemiselmis andersenii... [more]
A0A7S1SH10_9CHLO1.040e-733.91Hypothetical protein n=1 Tax=Tetraselmis chuii Tax... [more]
A0A445ACG8_ARAHY2.010e-740.86Uncharacterized protein n=3 Tax=Arachis TaxID=3817... [more]
A0A834YQV3_9MAGN2.220e-741.94Uncharacterized protein n=1 Tax=Tetracentron sinen... [more]
A0A7S2IRI2_9EUKA3.760e-750.77Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A6T7YBG7_9CRYP4.220e-735.05Hypothetical protein n=1 Tax=Cryptomonas curvata T... [more]
A0A1S4BHU9_TOBAC4.980e-739.78uncharacterized protein LOC107808434 n=12 Tax=Sola... [more]
A0A1U8DSZ5_CAPAN6.470e-741.11uncharacterized protein LOC107839242 n=4 Tax=Capsi... [more]
A0A835LU59_9MAGN6.630e-737.72Uncharacterized protein n=1 Tax=Coptis chinensis T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp6_S_contig87contigEcto-sp6_S_contig87:334963..337481 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species6 EcLAC_371 OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score361.7
Seed ortholog evalue2.1e-97
Seed eggNOG ortholog2880.D7FZB4
Model size1511
Hectar predicted targeting categorymitochondrion
Exons2
EggNOG OGs2D19J@1,2S4VG@2759
Ec32 ortholog descriptionRibosomal protein L28/L40, mitochondrial
Ec32 orthologEc-07_001600.1
Cds size573
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682350496.673128-CDS-Ecto-sp6_S_contig87:334962..3352221682350496.673128-CDS-Ecto-sp6_S_contig87:334962..335222Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig87 334963..335222 +
1682350496.6917584-CDS-Ecto-sp6_S_contig87:336230..3365431682350496.6917584-CDS-Ecto-sp6_S_contig87:336230..336543Ectocarpus species6 EcLAC_371CDSEcto-sp6_S_contig87 336231..336543 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1682350496.7045157-UTR-Ecto-sp6_S_contig87:336543..3374811682350496.7045157-UTR-Ecto-sp6_S_contig87:336543..337481Ectocarpus species6 EcLAC_371UTREcto-sp6_S_contig87 336544..337481 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp6_S_contig87.17811.1prot_Ecto-sp6_S_contig87.17811.1Ectocarpus species6 EcLAC_371polypeptideEcto-sp6_S_contig87 334963..336543 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp6_S_contig87.17811.1

>prot_Ecto-sp6_S_contig87.17811.1 ID=prot_Ecto-sp6_S_contig87.17811.1|Name=mRNA_Ecto-sp6_S_contig87.17811.1|organism=Ectocarpus species6 EcLAC_371|type=polypeptide|length=191bp
MLRPAVSTLSGRSVAAAVSRSSFPGVCAAGDRLERSRTAGSGGVVGCTRG
MMMAAKPGKIKKERSGKALDKKTMTLLKLLDARPEPMPELSAEEKQRNFD
IGRRYNRKMATSHNERMADLTTKIKLRDDAIAHLPELLQHAARQPDRNPP
PLNRRVATYTPPIPGFDKKDYGLSDDEDADAQLNRFRDGY*
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mRNA from alignment at Ecto-sp6_S_contig87:334963..337481+

Legend: CDSpolypeptideUTR
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp6_S_contig87.17811.1 ID=mRNA_Ecto-sp6_S_contig87.17811.1|Name=mRNA_Ecto-sp6_S_contig87.17811.1|organism=Ectocarpus species6 EcLAC_371|type=mRNA|length=2519bp|location=Sequence derived from alignment at Ecto-sp6_S_contig87:334963..337481+ (Ectocarpus species6 EcLAC_371)
ATGTTGCGACCCGCTGTCAGCACCCTATCGGGAAGGAGTGTTGCTGCTGC
CGTTTCCCGAAGTTCGTTTCCTGGAGTATGCGCTGCTGGTGATCGGTTAG
AGAGGAGTAGGACCGCGGGGAGTGGAGGGGTTGTTGGCTGCACACGCGGG
ATGATGATGGCAGCCAAGCCAGGCAAGATCAAGAAGGAGCGGTCGGGGAA
GGCCCTGGACAAGAAGACCATGACTCTCCTGAAGCTGCTTGATGCACGAC
CTGAGCCAATGTGAGAGGTTCTACACGTGGGTAGAGTAGGTGTGTGGTTT
GGACGAGCAAAAGTTTGTTCCAGGAGAGAAGGACGGACAGCACTCCCACA
TACTCTTTGCATCAACTTGGCCTCTTCACCTTTCACAATGGCGCTCACAC
CCTTCACGCGCCGTCTGTACGCACCAGGGCTCCATCGGTTCATGTGTACA
TTGTACTGCTGTCTCCCTGCCCTCCCCTAACGTCTCATACTTGTGTCTTG
GGAAATGGCTTGTCCGTGCATTGTGTGCACCTGGTTGCCGCACCCCGAAA
CATGTGGATACCGCCGGGAAGGGCTGCTGGGGCAAGCAAGCCACAGCCGT
CGGGCAGGCTCAAGTTCTTGCTGTTGTTGCTAGATGTGACCTCCTTGCCG
CTGAGAATCTCTGATTGTGTTGCAGTCGGCTCCCAATTCATCGTAAACCG
GCGGTGTCGTTTTGTATGTTCGATAAGGCGCAAACTGGAGCGTGCGGTGG
CAACATTGAGCACTCAACACCTCCAAAACCTGTGGTACCTCTGAGTCTAC
ACAGCAATAGCAGCAGCAGCTTTGTGGCAGCAGCTCCGGTGCTGTGTCTA
TCAGAGGACCATATTGCTCGCTTGTTGCGTTTGAGTGTCGGCGGAGGAAC
GGTGCGTGCGTATTGTATCAATCAACACAGCAGCAGTCACTCTGTTGAAC
ACGATTGATTCCTTTGTCCTGGACACCGAGAAAGTTTTCATTTCACGCAA
ACAGCGGCAACGCTCGAGCGAGACTGATGTAACACAGTCTATGTAGCAAG
CATGCTTTTGGTCTCTTGTACAAAACAGTAACTTGCAGGTCCCGTAGGTG
GGCCCCTTACAAACGTGCAGCGTTCGCCGACGGTAGGGTAGCGCATCCCC
CCCCTCCAAGATAGACAACTCAACGAACGCCCGAAACAAACCGCCGCCGT
TGCTGCAACTCCCAACCCCCCCAACCCCCCCCCCGCGCGCGCTGGCACTT
GGGCCTCGCATATACCAGGCCGGAACTCTCGGCGGAAGAAAAGCAGCGAA
ATTTCGACATCGGGCGGCGATACAATAGGAAGATGGCCACGAGCCACAAC
GAGAGGATGGCTGACCTAACAACCAAGATCAAGCTGAGGGACGACGCGAT
CGCGCACCTGCCGGAGCTACTGCAGCACGCGGCGAGACAGCCGGACAGGA
ACCCGCCGCCGCTGAACCGGAGGGTGGCCACCTACACCCCGCCCATCCCC
GGGTTCGACAAGAAGGACTACGGCCTCTCGGACGACGAGGACGCGGATGC
GCAGCTGAATCGCTTCCGGGATGGTTACTGAGTGTTTTGGCCTTTTTGTT
CATTTCTGTCCCTCCGTGTACTGTAGTCGCTGCTTGCTTTTTGGGTAGAG
AGAAGTCTACAGCAGTAGTCGCTCGGATTGGCTTTGGCTCGGCGCAGCCA
CTGGATGGATTCCCACCATAGATGAGCCAAAGAGCGTCTACCCTGGAGGG
AAAATGATGCGGAATTAGAGGCACACGTGGGACCGGCTCTCAGGCAGGAG
CATCGCATTTTGCAGGCGCTTCGGGAACGGTGCGGCATTGCTTGCAAGAG
TGCGTTATCCGCTCTCCTGGGATTTGAGGAAACGGATTCGCTTTTCTAAG
CCGGGGGTTCGTCGCCCGCCGCAAGTGTCTTGAGTTGTGCTGCCTTGTTG
CTGTTGTGGGACGACGTCGTGTTCGGACAACAAAATCTCCAGCGCCCGAC
GTATGGCTGCTGTGATGGACAAGCGGATGGGCATTCTGTGCGTGCCCCTT
TTGAGCGGGCTTTTTAAGCTTGGCGTCGCGAGAAAGTGCCGCCCACAATC
GAACCTCAAGTTTCGAGTGAAGTGCACATACTTTCGACGGATTGGTACAT
CGCCGTTCGTTTTTCCCTGCTCAGGGGAATGTTGGTGTAGAATAAAGTTT
GTATGCCCCGCCTCTCGCTTTTTTCTTGCCTATTGTGAGTACGTTTGCAT
CGAAAAAAGAAGATATATACATGAAGTATGCATTCTTTGGAGGGCCCTAC
TGCTGTGCTCTCGATGACCCTTGCGAGGGAAGACAAACACGACAGCATGC
GGGATTCTTGCGCGGACACCGACTTCTCCACAAGGTCTACGGACAGCTGA
GGTTTACCCGCAGGGAGGCCTCAGCTGCCGGTGGACCTCGTGGAACATGG
GCTGCTATGTACATATCGGCGCCATGCTGTCATCCGGGGTCCAATCACTT
AAGTGGGAGATTTGTGACG
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Coding sequence (CDS) from alignment at Ecto-sp6_S_contig87:334963..337481+

>mRNA_Ecto-sp6_S_contig87.17811.1 ID=mRNA_Ecto-sp6_S_contig87.17811.1|Name=mRNA_Ecto-sp6_S_contig87.17811.1|organism=Ectocarpus species6 EcLAC_371|type=CDS|length=573bp|location=Sequence derived from alignment at Ecto-sp6_S_contig87:334963..337481+ (Ectocarpus species6 EcLAC_371)
ATGTTGCGACCCGCTGTCAGCACCCTATCGGGAAGGAGTGTTGCTGCTGC
CGTTTCCCGAAGTTCGTTTCCTGGAGTATGCGCTGCTGGTGATCGGTTAG
AGAGGAGTAGGACCGCGGGGAGTGGAGGGGTTGTTGGCTGCACACGCGGG
ATGATGATGGCAGCCAAGCCAGGCAAGATCAAGAAGGAGCGGTCGGGGAA
GGCCCTGGACAAGAAGACCATGACTCTCCTGAAGCTGCTTGATGCACGAC
CTGAGCCAATGCCGGAACTCTCGGCGGAAGAAAAGCAGCGAAATTTCGAC
ATCGGGCGGCGATACAATAGGAAGATGGCCACGAGCCACAACGAGAGGAT
GGCTGACCTAACAACCAAGATCAAGCTGAGGGACGACGCGATCGCGCACC
TGCCGGAGCTACTGCAGCACGCGGCGAGACAGCCGGACAGGAACCCGCCG
CCGCTGAACCGGAGGGTGGCCACCTACACCCCGCCCATCCCCGGGTTCGA
CAAGAAGGACTACGGCCTCTCGGACGACGAGGACGCGGATGCGCAGCTGA
ATCGCTTCCGGGATGGTTACTGA
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