mRNA_Ecto-sp13_S_contig148.3660.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig148.3660.1
Unique NamemRNA_Ecto-sp13_S_contig148.3660.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig148.3660.1 vs. uniprot
Match: D7FNM3_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FNM3_ECTSI)

HSP 1 Score: 549 bits (1415), Expect = 7.790e-189
Identity = 282/326 (86.50%), Postives = 294/326 (90.18%), Query Frame = 1 
Query:    1 MVKSSDVNHQDHKPRTKYFLTTGGRVVRVNHDPDAGNRLIVPTEDELKTILYDQTDARNRGNGHRPRDNIDFVALHANRRPRRKGSKKGVGNNQRTYEDNCSPVVVNGEEFCLSDRSDIRRERGVKSDRCLRLKVNRPSPKHHDFGEEDDVSLFLDSFSASEMPGQHPAAAVETAVTRQSSPTSLCPDDGVLEGQARKAEAVVLDTTQTTLEMSRPLAKVDLYSILAKAPPAEPRSTAFHRPVGGTGRSVDPTMSNKAGMKRHKREQGSLRRLARSVVVGGDERPAYETALRLTPREEVGGYLKRDPLGDEVAELNSKLQESLRRV 978
            M+KS DVNHQDHKPRTKYFLT GGRVVRVNHDPDAGNR IVPTEDELKTILYDQTDARNRGNGHR RDNIDFVALHANRRPRRKGSK GV NNQRTYE++ SPV VNGE     DR  IRRERG+KSDRCLR K NRPSPKHHD GEE+DVSLFLDSFSASEMPGQHPAAAV+TAVT QS PTSLC DDGVLEGQARKAEAVV DTTQTT+EMS+PLAK+DLYSILAKAPP  PRSTA HRPV G G SVDPTMSNKAGMKRHKREQGSLRRLA SVVVGGDERPA+ETALRL PREEVGGYLKRDPLGDEVAEL +KLQESLR +
Sbjct:    1 MLKSFDVNHQDHKPRTKYFLTVGGRVVRVNHDPDAGNRQIVPTEDELKTILYDQTDARNRGNGHRRRDNIDFVALHANRRPRRKGSKNGVENNQRTYENSNSPVGVNGE-----DRPHIRRERGIKSDRCLRPKANRPSPKHHDSGEEEDVSLFLDSFSASEMPGQHPAAAVQTAVTLQSPPTSLCLDDGVLEGQARKAEAVVADTTQTTMEMSKPLAKIDLYSILAKAPPVAPRSTASHRPVDGIGCSVDPTMSNKAGMKRHKREQGSLRRLASSVVVGGDERPAFETALRLNPREEVGGYLKRDPLGDEVAELTAKLQESLRSI 321
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig148.3660.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 1
Match NameE-valueIdentityDescription
D7FNM3_ECTSI7.790e-18986.50Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig148contigEcto-sp13_S_contig148:17092..21702 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Model size2196
Hectar predicted targeting categoryother localisation
Exons7
Cds size2196
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig148.3660.1prot_Ecto-sp13_S_contig148.3660.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig148 17092..21702 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462789.4367282-CDS-Ecto-sp13_S_contig148:17091..172531681462789.4367282-CDS-Ecto-sp13_S_contig148:17091..17253Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 17092..17253 +
1681462789.4619532-CDS-Ecto-sp13_S_contig148:17839..183341681462789.4619532-CDS-Ecto-sp13_S_contig148:17839..18334Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 17840..18334 +
1681462789.4773448-CDS-Ecto-sp13_S_contig148:18690..190071681462789.4773448-CDS-Ecto-sp13_S_contig148:18690..19007Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 18691..19007 +
1681462789.4880419-CDS-Ecto-sp13_S_contig148:19433..204181681462789.4880419-CDS-Ecto-sp13_S_contig148:19433..20418Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 19434..20418 +
1681462789.5001056-CDS-Ecto-sp13_S_contig148:20768..208641681462789.5001056-CDS-Ecto-sp13_S_contig148:20768..20864Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 20769..20864 +
1681462789.5098932-CDS-Ecto-sp13_S_contig148:21167..212471681462789.5098932-CDS-Ecto-sp13_S_contig148:21167..21247Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 21168..21247 +
1681462789.5221164-CDS-Ecto-sp13_S_contig148:21641..217021681462789.5221164-CDS-Ecto-sp13_S_contig148:21641..21702Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig148 21642..21702 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig148.3660.1

>prot_Ecto-sp13_S_contig148.3660.1 ID=prot_Ecto-sp13_S_contig148.3660.1|Name=mRNA_Ecto-sp13_S_contig148.3660.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=732bp
MVKSSDVNHQDHKPRTKYFLTTGGRVVRVNHDPDAGNRLIVPTEDELKTI
LYDQTDARNRGNGHRPRDNIDFVALHANRRPRRKGSKKGVGNNQRTYEDN
CSPVVVNGEEFCLSDRSDIRRERGVKSDRCLRLKVNRPSPKHHDFGEEDD
VSLFLDSFSASEMPGQHPAAAVETAVTRQSSPTSLCPDDGVLEGQARKAE
AVVLDTTQTTLEMSRPLAKVDLYSILAKAPPAEPRSTAFHRPVGGTGRSV
DPTMSNKAGMKRHKREQGSLRRLARSVVVGGDERPAYETALRLTPREEVG
GYLKRDPLGDEVAELNSKLQESLRRVTQDTQRHKRRQQVGVARHYAAILG
DRTRPLNIAKNMPTHGRDEDNIDDNNDTDATSSHDSRRYRATTDDRDNGR
AADVFQRSCRRNRGRIEDFAKSNRTCSSTRISTRHSSGKQADSETSPTRG
GRDSTSGAGLEVGRAGVRWLPDDEAELHSPVTGQPIGCRGKHDRQAPQQE
QPQDDVLDSGTTDSDYGEEPFESANGAPESVSGSVAAAPSVVTTEGGVLG
MNLDVAMESKEHVGRKNGDGVDSEREDYYLGHEATGEDRVLLSKDGAPPS
SAAAAASTAAAAAAALRIEACWRGFVGRCFAKRLLRSGLLDALRSLGGGK
VSKVVSLTDVEREDRRGLNRALRLATQSRRQLPPQAHIFECVRFVKSLQR
RRARVVEARKRRWIAERRQRRESAKNVPSGLL
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mRNA from alignment at Ecto-sp13_S_contig148:17092..21702+

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig148.3660.1 ID=mRNA_Ecto-sp13_S_contig148.3660.1|Name=mRNA_Ecto-sp13_S_contig148.3660.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4611bp|location=Sequence derived from alignment at Ecto-sp13_S_contig148:17092..21702+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGTGAAATCATCCGACGTCAACCACCAGGACCACAAGCCCCGGACGAA
GTATTTTCTAACGACTGGTGGGCGGGTGGTGAGAGTCAACCATGACCCTG
ACGCTGGCAATAGGCTAATCGTGCCTACCGAGGACGAGCTAAAGACAATT
CTCTATGACCAGGTGAGTAATGGACGGCATACGCAACAGATCATTTCAAG
ACATATTTCAATCGTCTGTCTCCGTATCCTCCATAGTCCAGCCTGCACCT
CACGATGCATGCAGCACTTCAAAAGTGGGCGGTTGCCGTCTTCACAAATG
TGCTACCCACCCCCCAAACAACTCGGTACCTGTAACGCGTGCACCAACGG
AGCCCGAGCCATGTATCTATGGCTCTGTCGTAAGTATCATTTGCTCTACC
AGGCTCTACTGTTGCTTTTCTGACATCTCAAACGGGCTGAATTACATAGA
ACCTCGGGATCAGAACGGTTGTTACGTGAAGACAACGTCAACCACAACCA
CAACCACAACCACAACAACAACAACAACAACGACGACGATGATGACAAGT
TATCGTCTTTCGTTGACGTCGTGATTCAGAAGTCCATGTCCAAACGAAAC
AAGAGGAATTCTAGAGAGTAGATGCGCAACTCGAACGAATCACCAACCGT
GAGTGCATGTAAAATCTTGGAGATACACCACAACAATGTAAAGCGAACAT
CTCCTTGTGATGTGATGTGTGCCCTCTTCGTTGGCCTTTTCCCCTCAGAC
GGATGCTAGGAATAGGGGGAATGGACACCGACCACGTGACAACATTGACT
TTGTGGCCCTTCATGCTAACCGCAGACCAAGACGAAAAGGCAGCAAAAAG
GGGGTCGGAAACAACCAGCGCACATACGAGGACAACTGCAGTCCGGTTGT
TGTAAATGGTGAAGAGTTTTGTTTGTCAGACCGATCCGACATCCGGCGGG
AAAGGGGCGTAAAGTCCGACCGATGTCTCCGCCTGAAAGTGAACAGACCA
AGTCCGAAGCACCACGATTTTGGAGAAGAGGACGATGTCTCCTTATTCCT
GGACTCGTTCTCCGCTTCCGAGATGCCAGGACAGCATCCCGCAGCGGCAG
TAGAGACGGCAGTTACTCGCCAATCGTCCCCGACGTCGCTCTGTCCGGAC
GACGGTGTGCTCGAGGGGCAGGCGCGTAAAGCTGAAGCTGTTGTCCTCGA
CACCACGCAAACCACGCTGGAGATGAGTAGGCCACTCGCAAAGGTAAGAG
GCAAGCATGCTTTCATTAAATGCCAAGGGTGGAGACCAAAAGCTTCACCC
AGTGATCTTGTGCAATGGAATCAGACTGCCGTTAGATCTTGGCGGGTTGG
CTGTTACTTCGTTGCCATTCGTGTTAGTGAAAGACCTCTGGCTCCTCCGC
AACTGGTCCTCGAGCTCTTTGTATATGCATCTATCTCAAGGAAGAGCTTC
CTGGCACTGTGGGGCAATAAGGACGTCACGGCTCAAATCTACCTCCCTGC
CGAATAACGTGGTTCCTATCGTGTCCAATGAACCGTATTTCAACAAATGC
TCAGTCCATGGCTGACCCCCCTCCCCCCCCCTACGGCTGCTACTCCCAGG
TTGATCTGTACAGCATCCTAGCCAAGGCTCCTCCTGCCGAGCCACGTAGC
ACAGCATTCCACCGTCCGGTCGGCGGGACCGGCCGCAGCGTTGACCCAAC
CATGTCGAACAAGGCCGGAATGAAGCGACACAAGCGAGAGCAAGGTTCCT
TGCGCAGACTCGCGAGGAGTGTCGTCGTCGGGGGAGACGAACGACCAGCG
TATGAAACAGCACTGCGGTTGACCCCGCGAGAGGAGGTGGGCGGATACCT
CAAGAGAGACCCGCTGGGAGACGAAGTCGCCGAGTTAAACTCCAAGCTTC
AGGAAAGCCTTCGAAGGTGAGGAGTGGGGAAAACCGAGAGCAGGTGCGGT
CGTGGCGAGCATGTTTTGTTGGGTATCTCTAGCAGCCGCCGTCGTCGGTG
TATCGAACGCGGGCGGTTGGATTACCCAGATGATGTCGATAAATGTGCTT
GGTTTTCGGTATTTCTTGGAAGTACAATGCTCAATGGCCGTGCCATGCTA
TGACCCACGGCATCAAGCGCCTCATTGAGTGCGTTTGGTTTGATGTTGCT
ATCTACGTTTGTCGTCGTAGTTCTGGTAGAGCGCCCCAGTCAAGCCTCGC
GCTAAGCCCGGTGGGAGAAGCAATTGTTCGGTCTGCGTCGGTATGTGTTT
GTGCCGACCTTTCTCGGTTGGTTCAACGAAGAAAAGTAACACAGTTTCTT
TCGCTTTCCGCCTACATGTCCCAATCCCCCCCGTCCCGTCAGGGTCACAC
AAGACACGCAGAGACATAAACGTAGGCAACAAGTCGGCGTAGCCCGGCAT
TACGCGGCCATCCTTGGGGACAGGACCCGACCGCTTAACATAGCCAAGAA
TATGCCAACGCATGGCCGTGATGAGGATAACATTGACGACAACAACGACA
CTGATGCTACATCATCGCATGATTCTCGTCGTTATCGAGCAACAACAGAC
GATCGTGATAACGGTCGCGCGGCCGACGTCTTTCAGCGCTCGTGCCGTCG
TAACCGAGGACGAATAGAAGACTTCGCAAAGAGTAATCGAACATGCAGCA
GCACAAGAATATCAACCAGGCATAGCAGCGGAAAGCAAGCCGATTCCGAA
ACCAGTCCAACAAGGGGGGGTCGCGATTCTACCTCTGGGGCAGGTCTTGA
GGTCGGTCGGGCTGGCGTGAGATGGCTGCCTGATGATGAGGCGGAATTGC
ATTCTCCCGTGACGGGGCAACCAATCGGCTGCAGAGGAAAACATGACCGT
CAGGCCCCGCAACAGGAACAACCCCAGGACGATGTTCTCGATTCCGGGAC
CACAGACAGCGACTATGGGGAAGAACCGTTCGAATCGGCAAACGGTGCGC
CGGAAAGCGTTTCGGGGTCTGTGGCAGCGGCCCCGAGCGTCGTCACCACA
GAAGGAGGCGTTTTAGGGATGAACTTGGATGTCGCGATGGAAAGCAAGGA
ACACGTAGGCCGAAAAAATGGTGATGGTGTCGACAGCGAGCGAGAAGACT
ATTATTTGGGCCACGAGGCCACGGGGGAGGACCGGGTACTGCTGTCCAAA
GACGGTGCTCCTCCTTCTTCTGCTGCTGCTGCTGCTTCTACTGCTGCTGC
TGCTGCTGCTGCGCTGAGGATCGAGGCTTGTTGGCGAGGTTTCGTCGGAA
GATGTTTCGCCAAACGCTTGCTACGATCTGGACTTCTCGATGCTCTACGC
AGTCTCGGCGGTGGGAAGGTTTCCAAGGTGAGACATGCAGAGTTTGCAGA
GACGTAGATCGACTGGTCACTCTGCCAAGTATCTCCTACTGAGGTACGGG
CTGTGCCCTCCCTACGCATCGAGCTTGCGCGACATTCTTCGAAGGCATGG
GTCCACGTAAAGCTCTCTGACAAACGCGGAGCCATCCGGCGACACCCGAT
GCGCTCTGTGGAGTTCAGGCGTTATGCAGACATCAAGGTTTCGCAAAAAT
GGAGGAGAGCGGAATGAGATATGATACGGCACGCTATAATATTCGTCGCT
GGCCATCCGACGGACCTTGTCCAACCGCTCGTGGTCGTTTCGTGGCTTTT
CGTGTGGTGTGAATTGGGGGAATGAAGGTCGTGTCGCTGACAGACGTTGA
AAGAGAAGATCGACGGGGCCTCAATCGTGCTCTTCGACTGGCAACCCAAT
CCCGGCGTCAACTACCGCCGCAGGTACTTGAGTGACAACGAGGGTGTGCC
TAGGTAGCTCTGGACCGAAATCTTTTCCGTTTTGTCTGCACTGACACACA
GCAGTAAGAAAAACGTATGCGTGTCCATGCCTTATATGGTGTCTTATCAG
TCTTGCAGAATATTCCAGACGGTGTAGTTGAAGTACACGACTTCTAGACT
TTTGTTCGCTCGTTGTACCCACCCAATATTTTATTTGGATTCCCCCATCT
CCGCGACACTTGTTCATGCCATACCCATATTTCTTCGTTTCTTCCTACCG
ACTGCGGCTCTCGTGTGGTTCAAAAGGCGCATATCTTCGAGTGTGTTCGC
TTCGTGAAGTCATTGCAGAGGCGGCGTGCCCGCGTTGTGGAAGCGAGGAA
GAGGAGGTGGGATCCGGAAAAGATTGAGACGGGCGTGCGTTTTTTCTCGA
GCTTCGTTTAACCGCTTCGTGAAGGGTGGCACCATTCGCGATGAAAACCA
TTTCCCGAGCTAGTAGCTCAGAATCTCCCTGGCTGCAACTACACGCAGTT
GCAGCTCGGTCCTAAAGTCAACGACAGTTCGATGCAGGCGCTTCGAAATA
TCTGTCGAGTGAAAAAGACACCTCCAAGACCTATTTCCAAGGTACACGAA
CGTAGTTGCATGCAACCATCTTGTAGATGGAGGCATGGAGCCCACTGTTG
GACAATGTGACGGAGTTCATCCCGCAGTGATTTGCCCCGCTTCCATGCCC
CTCATTGTATTCCCTCTCTTGTAATCTCTCGCGGGGCCCCTTTCTGGTAG
ATGGATAGCGGAGAGGCGTCAGAGAAGGGAGAGCGCCAAGAACGTTCCTT
CCGGGCTTCTT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig148:17092..21702+

>mRNA_Ecto-sp13_S_contig148.3660.1 ID=mRNA_Ecto-sp13_S_contig148.3660.1|Name=mRNA_Ecto-sp13_S_contig148.3660.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=2196bp|location=Sequence derived from alignment at Ecto-sp13_S_contig148:17092..21702+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGTGAAATCATCCGACGTCAACCACCAGGACCACAAGCCCCGGACGAA
GTATTTTCTAACGACTGGTGGGCGGGTGGTGAGAGTCAACCATGACCCTG
ACGCTGGCAATAGGCTAATCGTGCCTACCGAGGACGAGCTAAAGACAATT
CTCTATGACCAGACGGATGCTAGGAATAGGGGGAATGGACACCGACCACG
TGACAACATTGACTTTGTGGCCCTTCATGCTAACCGCAGACCAAGACGAA
AAGGCAGCAAAAAGGGGGTCGGAAACAACCAGCGCACATACGAGGACAAC
TGCAGTCCGGTTGTTGTAAATGGTGAAGAGTTTTGTTTGTCAGACCGATC
CGACATCCGGCGGGAAAGGGGCGTAAAGTCCGACCGATGTCTCCGCCTGA
AAGTGAACAGACCAAGTCCGAAGCACCACGATTTTGGAGAAGAGGACGAT
GTCTCCTTATTCCTGGACTCGTTCTCCGCTTCCGAGATGCCAGGACAGCA
TCCCGCAGCGGCAGTAGAGACGGCAGTTACTCGCCAATCGTCCCCGACGT
CGCTCTGTCCGGACGACGGTGTGCTCGAGGGGCAGGCGCGTAAAGCTGAA
GCTGTTGTCCTCGACACCACGCAAACCACGCTGGAGATGAGTAGGCCACT
CGCAAAGGTTGATCTGTACAGCATCCTAGCCAAGGCTCCTCCTGCCGAGC
CACGTAGCACAGCATTCCACCGTCCGGTCGGCGGGACCGGCCGCAGCGTT
GACCCAACCATGTCGAACAAGGCCGGAATGAAGCGACACAAGCGAGAGCA
AGGTTCCTTGCGCAGACTCGCGAGGAGTGTCGTCGTCGGGGGAGACGAAC
GACCAGCGTATGAAACAGCACTGCGGTTGACCCCGCGAGAGGAGGTGGGC
GGATACCTCAAGAGAGACCCGCTGGGAGACGAAGTCGCCGAGTTAAACTC
CAAGCTTCAGGAAAGCCTTCGAAGGGTCACACAAGACACGCAGAGACATA
AACGTAGGCAACAAGTCGGCGTAGCCCGGCATTACGCGGCCATCCTTGGG
GACAGGACCCGACCGCTTAACATAGCCAAGAATATGCCAACGCATGGCCG
TGATGAGGATAACATTGACGACAACAACGACACTGATGCTACATCATCGC
ATGATTCTCGTCGTTATCGAGCAACAACAGACGATCGTGATAACGGTCGC
GCGGCCGACGTCTTTCAGCGCTCGTGCCGTCGTAACCGAGGACGAATAGA
AGACTTCGCAAAGAGTAATCGAACATGCAGCAGCACAAGAATATCAACCA
GGCATAGCAGCGGAAAGCAAGCCGATTCCGAAACCAGTCCAACAAGGGGG
GGTCGCGATTCTACCTCTGGGGCAGGTCTTGAGGTCGGTCGGGCTGGCGT
GAGATGGCTGCCTGATGATGAGGCGGAATTGCATTCTCCCGTGACGGGGC
AACCAATCGGCTGCAGAGGAAAACATGACCGTCAGGCCCCGCAACAGGAA
CAACCCCAGGACGATGTTCTCGATTCCGGGACCACAGACAGCGACTATGG
GGAAGAACCGTTCGAATCGGCAAACGGTGCGCCGGAAAGCGTTTCGGGGT
CTGTGGCAGCGGCCCCGAGCGTCGTCACCACAGAAGGAGGCGTTTTAGGG
ATGAACTTGGATGTCGCGATGGAAAGCAAGGAACACGTAGGCCGAAAAAA
TGGTGATGGTGTCGACAGCGAGCGAGAAGACTATTATTTGGGCCACGAGG
CCACGGGGGAGGACCGGGTACTGCTGTCCAAAGACGGTGCTCCTCCTTCT
TCTGCTGCTGCTGCTGCTTCTACTGCTGCTGCTGCTGCTGCTGCGCTGAG
GATCGAGGCTTGTTGGCGAGGTTTCGTCGGAAGATGTTTCGCCAAACGCT
TGCTACGATCTGGACTTCTCGATGCTCTACGCAGTCTCGGCGGTGGGAAG
GTTTCCAAGGTCGTGTCGCTGACAGACGTTGAAAGAGAAGATCGACGGGG
CCTCAATCGTGCTCTTCGACTGGCAACCCAATCCCGGCGTCAACTACCGC
CGCAGGCGCATATCTTCGAGTGTGTTCGCTTCGTGAAGTCATTGCAGAGG
CGGCGTGCCCGCGTTGTGGAAGCGAGGAAGAGGAGATGGATAGCGGAGAG
GCGTCAGAGAAGGGAGAGCGCCAAGAACGTTCCTTCCGGGCTTCTT
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