mRNA_Ecto-sp13_S_contig13872.3028.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig13872.3028.1
Unique NamemRNA_Ecto-sp13_S_contig13872.3028.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FTZ5_ECTSI (Signal transducer, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ5_ECTSI)

HSP 1 Score: 253 bits (646), Expect = 5.180e-79
Identity = 148/203 (72.91%), Postives = 154/203 (75.86%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSPNDTEVPSPSSPCGVLGPVRRLSLVGRMLLSSSLGPARASLSSAAPPDASTGGGERGRGLGDNNGSRSTQDEEKGRDEDGEMAVVKTGGGNXXXXXXXXGGTS 609
            +F  QAATMRIVDQ+IREGAPDQVNISGECRD+ILSTH TA+DIFDRARAEVLDVMETNFQTDFVETEGFRRIV+AAELEQRQIRLLQAEGFLLPNTGTSP DTEV SPSSP GVLGP RRLSLVGRML SSS+GPAR SLSSAAP DA TG                             MAV KTGGGN        GGTS
Sbjct:  290 YFARQAATMRIVDQYIREGAPDQVNISGECRDEILSTHVTAYDIFDRARAEVLDVMETNFQTDFVETEGFRRIVNAAELEQRQIRLLQAEGFLLPNTGTSPTDTEVLSPSSPDGVLGPFRRLSLVGRMLFSSSVGPARTSLSSAAPQDAGTGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXMAVAKTGGGNVPGGGGEGGGTS 492
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FTZ7_ECTSI (Similar to regulator of G-protein signaling 16 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ7_ECTSI)

HSP 1 Score: 166 bits (421), Expect = 6.700e-46
Identity = 97/154 (62.99%), Postives = 118/154 (76.62%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSPNDTEVP-SPSSPCG-VLGPVRRLSLVGRMLLSSSLGPARASLSSAAPPDASTG 456
            +F  QAATMRIV+Q+IREG+PDQVNISGECR++ILST  TA+DIFDRARAEVL VMETNFQ DF+ETEGFRRIVDA+ELEQRQ+RLL+AEG L+P+  T+P     P S  SP G +L   RRL LVGR+   SS      ++S ++ P A +G
Sbjct:  287 YFGRQAATMRIVEQYIREGSPDQVNISGECREEILSTDVTAYDIFDRARAEVLAVMETNFQRDFIETEGFRRIVDASELEQRQMRLLRAEGVLVPSASTTPVRLAAPPSIFSPRGRLLDSCRRLPLVGRLFSFSSPSAVAHAVSRSSTPGAGSG 440
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: A0A6H5K0N5_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K0N5_9PHAE)

HSP 1 Score: 163 bits (413), Expect = 3.730e-45
Identity = 92/135 (68.15%), Postives = 109/135 (80.74%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSPNDTEVP-SPSSPCG-VLGPVRRLSLVGRMLLSSS 399
            +F  QAATMRIV+Q+IREG PDQVNISGECR++ILST  TA+DIFDRARAEVL VMETNFQ DFVETEGFRRIVDA+ELEQR++RLL+AEG L+P+  T+P     P S  SP G ++  +R L LVGR+  SSS
Sbjct:  236 YFARQAATMRIVEQYIREGGPDQVNISGECREEILSTDVTAYDIFDRARAEVLAVMETNFQRDFVETEGFRRIVDASELEQREMRLLRAEGVLVPSASTTPVRLAAPRSIFSPRGRLVDSLRCLPLVGRLFSSSS 370
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FU02_ECTSI (Signal transducer, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU02_ECTSI)

HSP 1 Score: 148 bits (374), Expect = 6.020e-42
Identity = 77/101 (76.24%), Postives = 86/101 (85.15%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSP 303
            +F  QA TMRIV Q+IREGAPDQVNISGECR++ILST  TA+DIFDRAR EVL VMETNFQ +FVETEGFRRIVDAAELEQR+ RLL+A GFL P+   SP
Sbjct:   72 YFTRQAVTMRIVAQYIREGAPDQVNISGECREEILSTDVTAYDIFDRARVEVLTVMETNFQREFVETEGFRRIVDAAELEQRENRLLRAGGFLPPDAPPSP 172
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FU07_ECTSI (Signal transducer, putative n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FU07_ECTSI)

HSP 1 Score: 148 bits (373), Expect = 3.080e-41
Identity = 87/138 (63.04%), Postives = 103/138 (74.64%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLL----PNTGTSPNDTEVPSPSSPCGVLGPVRRLSLV-GRMLLSSS 399
            +F  QAATMRIVDQ+IREGA +QVNISG CR++ILST  TA+DIFD ARAEVL +METNFQ DFVETEGFRRI DA+ LEQ++IRLL+ EGFL+    P   + P  + V  P S  G+L    R SLV GR+L SSS
Sbjct:   82 YFPRQAATMRIVDQYIREGAAEQVNISGRCREEILSTDVTAYDIFDHARAEVLALMETNFQQDFVETEGFRRIADASALEQQEIRLLRKEGFLVADASPTLNSPPPASSVTPPQS--GLLNKFTRFSLVVGRILPSSS 217
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: A0A6H5JJQ0_9PHAE (RGS domain-containing protein n=3 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JJQ0_9PHAE)

HSP 1 Score: 149 bits (375), Expect = 3.420e-39
Identity = 76/100 (76.00%), Postives = 87/100 (87.00%), Query Frame = 1 
Query:    1 HFRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTS 300
            +F  QA TMRIV Q+IREGAPDQVNISGECR++ILSTH TA+DIFDRAR EVL VMETNFQ +FVETEGFRRIV+A+ELEQR+ RLL+A GFL P+T  S
Sbjct:  355 YFARQAVTMRIVAQYIREGAPDQVNISGECREEILSTHVTAYDIFDRARVEVLTVMETNFQREFVETEGFRRIVEASELEQRENRLLRAGGFLPPDTPPS 454
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FIR7_ECTSI (RGS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIR7_ECTSI)

HSP 1 Score: 132 bits (333), Expect = 5.230e-33
Identity = 72/114 (63.16%), Postives = 88/114 (77.19%), Query Frame = 1 
Query:    4 FRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFL---LPNTGTSPNDTEVPSPSSP 336
            F  QAATMRIVDQ+I+EGA DQVN+SG CR+ IL+T  TAFDIFD ARAEVL VMETNF+ DFV TEGFRRI++A++ EQR++RLL+A G L    P+ G+S  D    S +SP
Sbjct:   73 FERQAATMRIVDQYIKEGARDQVNVSGACREKILATDVTAFDIFDEARAEVLAVMETNFRRDFVTTEGFRRILNASKQEQRELRLLRAGGMLPLGTPSPGSSSADKTPSSSASP 186
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: A0A6H5KAT5_9PHAE (RGS domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KAT5_9PHAE)

HSP 1 Score: 134 bits (338), Expect = 6.660e-33
Identity = 73/111 (65.77%), Postives = 88/111 (79.28%), Query Frame = 1 
Query:    4 FRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSPNDT-EVPSPSS 333
            F  QAATMRIVDQ+I+EGA DQVN+SG CR+ IL+T  TAFDIFD ARAEVL VMETNF+ DFV TEGFRRI++A++ EQR++RLL+A G LLP    SP  + E  +PSS
Sbjct:  318 FERQAATMRIVDQYIKEGARDQVNVSGACREKILATDVTAFDIFDEARAEVLAVMETNFRRDFVTTEGFRRILNASKQEQRELRLLRAGGMLLPLGTPSPGSSYEDKTPSS 428
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FTZ9_ECTSI (RGS domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FTZ9_ECTSI)

HSP 1 Score: 130 bits (327), Expect = 2.020e-32
Identity = 67/100 (67.00%), Postives = 83/100 (83.00%), Query Frame = 1 
Query:    4 FRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSP 303
            F  QAATMRIV ++IR+GAP+QVNIS  CR++I+ST+ TA+DIFDRARAEVL VME  F+ +FVETEGFRRI +A+ELEQR+ RLL+A GFL P+T   P
Sbjct:  329 FGRQAATMRIVARYIRDGAPEQVNISEACREEIVSTNVTAYDIFDRARAEVLAVMEAEFKAEFVETEGFRRIANASELEQREKRLLRAGGFLPPSTPPGP 428
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BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Match: D7FIS3_ECTSI (Similar to retinally abundant regulator of G-protein signaling hRGS-r n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIS3_ECTSI)

HSP 1 Score: 130 bits (327), Expect = 3.250e-32
Identity = 69/100 (69.00%), Postives = 82/100 (82.00%), Query Frame = 1 
Query:    4 FRFQAATMRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMETNFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSP 303
            F  QAATMRIV+++IRE APDQVNIS ECR+ IL+T  TA+DIFD ARAEVL VMETNFQ +FV TEGFRRI DA+E+E R+IRLL+A G +LP+   SP
Sbjct:  165 FERQAATMRIVERYIREDAPDQVNISEECREKILATDVTAYDIFDEARAEVLVVMETNFQREFVTTEGFRRIADASEVEHREIRLLRAGG-MLPSASPSP 263
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig13872.3028.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 18
Match NameE-valueIdentityDescription
D7FTZ5_ECTSI5.180e-7972.91Signal transducer, putative n=1 Tax=Ectocarpus sil... [more]
D7FTZ7_ECTSI6.700e-4662.99Similar to regulator of G-protein signaling 16 n=1... [more]
A0A6H5K0N5_9PHAE3.730e-4568.15RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FU02_ECTSI6.020e-4276.24Signal transducer, putative n=1 Tax=Ectocarpus sil... [more]
D7FU07_ECTSI3.080e-4163.04Signal transducer, putative n=1 Tax=Ectocarpus sil... [more]
A0A6H5JJQ0_9PHAE3.420e-3976.00RGS domain-containing protein n=3 Tax=Ectocarpus T... [more]
D7FIR7_ECTSI5.230e-3363.16RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
A0A6H5KAT5_9PHAE6.660e-3365.77RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FTZ9_ECTSI2.020e-3267.00RGS domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7FIS3_ECTSI3.250e-3269.00Similar to retinally abundant regulator of G-prote... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig13872contigEcto-sp13_S_contig13872:3261..4329 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score162.5
Seed ortholog evalue1.9e-37
Seed eggNOG ortholog2880.D7FTZ7
Preferred nameRGS1
Model size612
KEGG koko:K16449
Hectar predicted targeting categoryother localisation
GOsGO:0001101,GO:0003674,GO:0004888,GO:0004930,GO:0005096,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005768,GO:0005886,GO:0006950,GO:0007154,GO:0007165,GO:0007186,GO:0008047,GO:0008150,GO:0009414,GO:0009415,GO:0009628,GO:0009719,GO:0009725,GO:0009737,GO:0009743,GO:0009746,GO:0009749,GO:0009756,GO:0009987,GO:0010008,GO:0010033,GO:0010035,GO:0010182,GO:0012505,GO:0016020,GO:0023052,GO:0030234,GO:0030695,GO:0031090,GO:0031410,GO:0031982,GO:0033993,GO:0034284,GO:0038023,GO:0042127,GO:0042221,GO:0043085,GO:0043087,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043547,GO:0044093,GO:0044422,GO:0044424,GO:0044433,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051336,GO:0051345,GO:0051716,GO:0060089,GO:0060589,GO:0065007,GO:0065009,GO:0070887,GO:0071310,GO:0071322,GO:0071944,GO:0097305,GO:0097708,GO:0098588,GO:0098772,GO:0098805,GO:1901700,GO:1901701
Exons3
EggNOG free text desc.Regulator of G protein signaling domain
EggNOG OGs2CNEE@1,2QVMV@2759
Cds size588
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462740.1779914-CDS-Ecto-sp13_S_contig13872:3260..37011681462740.1779914-CDS-Ecto-sp13_S_contig13872:3260..3701Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig13872 3261..3701 -
1681462740.1875563-CDS-Ecto-sp13_S_contig13872:3962..40641681462740.1875563-CDS-Ecto-sp13_S_contig13872:3962..4064Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig13872 3963..4064 -
1681462740.19574-CDS-Ecto-sp13_S_contig13872:4260..43051681462740.19574-CDS-Ecto-sp13_S_contig13872:4260..4305Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig13872 4261..4305 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462740.2043152-UTR-Ecto-sp13_S_contig13872:4305..43291681462740.2043152-UTR-Ecto-sp13_S_contig13872:4305..4329Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig13872 4306..4329 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig13872.3028.1prot_Ecto-sp13_S_contig13872.3028.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig13872 3261..4305 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig13872.3028.1

>prot_Ecto-sp13_S_contig13872.3028.1 ID=prot_Ecto-sp13_S_contig13872.3028.1|Name=mRNA_Ecto-sp13_S_contig13872.3028.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=196bp
MRIVDQFIREGAPDQVNISGECRDDILSTHATAFDIFDRARAEVLDVMET
NFQTDFVETEGFRRIVDAAELEQRQIRLLQAEGFLLPNTGTSPNDTEVPS
PSSPCGVLGPVRRLSLVGRMLLSSSLGPARASLSSAAPPDASTGGGERGR
GLGDNNGSRSTQDEEKGRDEDGEMAVVKTGGGNAPGGGGEGGGTS*
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mRNA from alignment at Ecto-sp13_S_contig13872:3261..4329-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig13872.3028.1 ID=mRNA_Ecto-sp13_S_contig13872.3028.1|Name=mRNA_Ecto-sp13_S_contig13872.3028.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1069bp|location=Sequence derived from alignment at Ecto-sp13_S_contig13872:3261..4329- (Ectocarpus species13 EcNAP12_S_4_19m)
CATTTTCGCTTTCAGGCGGCGACAATGCGCATTGTGGACCAATTCATCAG
AGAAGGAGCCCCCGACCAGGTGCGGAGAGATGTCAGGTACAATGTTTTTT
TGTGTGCTGGTTGTCTTTTTCGGTATCACGCTTCACCTATACCTGCACAC
GTTCACGAGCAACCCCCTGCTCGTCTTCAGCGTTCCTTCGTGTAATGCTA
TCAATGAAGCGCTCCCTTGCTCCTTTGTTGGGGGCGATTCTGCTGCGTGT
TTTTCGCATTCGTAGGTCAACATATCCGGGGAGTGCCGGGATGATATTTT
GTCGACGCATGCGACGGCGTTCGACATTTTCGACCGCGCCAGGGCCGAGG
TTCTGGACGTTATGGAGGTAAGGCAGACGAGAAGAGGAGTGCGTTTGTCA
AGCCACAGTCCCAAACTCCCTTTGCTCTCGGCAGGTCACAGTCTGGCATC
CCACGCTGCCACCTCACGTTTTGTTTTCAAGCGGTCTCGTGCCACTTACG
TCCACAACCGGCACCCGCCTTCGGGGCATTTCTGGCTCTCACGTCATAAG
AACATAAGAAACTCCCGTCCCCCTTCTCCACGTTCGACCCTTTCGCTGCT
CCAACCACATTGAAACCAACGAAAAAAGACCAACTTCCAGACGGATTTCG
TGGAGACGGAGGGGTTCCGTCGCATCGTCGACGCGGCCGAGCTGGAGCAG
AGACAGATCCGTCTCCTTCAGGCCGAGGGTTTCTTGCTGCCGAATACCGG
GACCTCACCGAATGACACGGAGGTTCCCTCTCCTTCCAGTCCGTGCGGCG
TGCTGGGCCCGGTAAGGCGCCTGTCCTTAGTGGGACGGATGTTGTTATCG
TCTTCATTAGGACCAGCTCGCGCTTCGTTGTCTTCGGCAGCGCCCCCAGA
TGCCAGCACCGGGGGCGGAGAGAGGGGCAGAGGTCTTGGTGACAACAACG
GGAGCAGGAGCACTCAGGATGAGGAAAAAGGGCGCGATGAGGATGGCGAA
ATGGCCGTTGTTAAGACTGGTGGCGGCAATGCACCTGGTGGCGGTGGTGA
AGGGGGAGGTACCTCGTAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig13872:3261..4329-

>mRNA_Ecto-sp13_S_contig13872.3028.1 ID=mRNA_Ecto-sp13_S_contig13872.3028.1|Name=mRNA_Ecto-sp13_S_contig13872.3028.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=588bp|location=Sequence derived from alignment at Ecto-sp13_S_contig13872:3261..4329- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCGCATTGTGGACCAATTCATCAGAGAAGGAGCCCCCGACCAGGTCAA
CATATCCGGGGAGTGCCGGGATGATATTTTGTCGACGCATGCGACGGCGT
TCGACATTTTCGACCGCGCCAGGGCCGAGGTTCTGGACGTTATGGAGACC
AACTTCCAGACGGATTTCGTGGAGACGGAGGGGTTCCGTCGCATCGTCGA
CGCGGCCGAGCTGGAGCAGAGACAGATCCGTCTCCTTCAGGCCGAGGGTT
TCTTGCTGCCGAATACCGGGACCTCACCGAATGACACGGAGGTTCCCTCT
CCTTCCAGTCCGTGCGGCGTGCTGGGCCCGGTAAGGCGCCTGTCCTTAGT
GGGACGGATGTTGTTATCGTCTTCATTAGGACCAGCTCGCGCTTCGTTGT
CTTCGGCAGCGCCCCCAGATGCCAGCACCGGGGGCGGAGAGAGGGGCAGA
GGTCTTGGTGACAACAACGGGAGCAGGAGCACTCAGGATGAGGAAAAAGG
GCGCGATGAGGATGGCGAAATGGCCGTTGTTAAGACTGGTGGCGGCAATG
CACCTGGTGGCGGTGGTGAAGGGGGAGGTACCTCGTAA
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