mRNA_Ecto-sp13_S_contig1331.2652.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_Ecto-sp13_S_contig1331.2652.1
Unique Name	mRNA_Ecto-sp13_S_contig1331.2652.1
Type	mRNA
Organism	Ectocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)

Homology

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BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot
Match: D8LHU4_ECTSI (Rap2 interacting protein x isoform 3 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHU4_ECTSI)

HSP 1 Score: 439 bits (1128), Expect = 5.540e-138
Identity = 231/246 (93.90%), Postives = 234/246 (95.12%), Query Frame = 1

Query:  115 MSWLSSIISGPQDSRESGKQVEARQRAATRARLVKNLRNAAQALAALQAKQQQEGVVPPQQAGGAMGMASETSGIPPRQHPPLIRGDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLTTGGGGAGRPGPHERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLEPCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVVIDTDQSDLDTPPRWPILEE 852
            MSWLSSIISGPQDSR+SGKQVEARQRAATRARLVKNLRNAAQALAALQAKQQQEGV PPQQAGGAMGM SET GIP RQ PPLI GDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLT GGGGAGR GP ERRLASVRNTVGAVASLS+VSTPLGRARAWIRQCLVCKCLE CVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDV IDTDQS L+TPPRWPILEE
Sbjct:    1 MSWLSSIISGPQDSRDSGKQVEARQRAATRARLVKNLRNAAQALAALQAKQQQEGVAPPQQAGGAMGMVSETGGIPRRQQPPLIGGDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLTIGGGGAGRQGPRERRLASVRNTVGAVASLSHVSTPLGRARAWIRQCLVCKCLESCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVAIDTDQSALNTPPRWPILEE 246

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BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot
Match: A0A6H5KE74_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KE74_9PHAE)

HSP 1 Score: 416 bits (1068), Expect = 1.140e-129
Identity = 217/230 (94.35%), Postives = 218/230 (94.78%), Query Frame = 1

Query:  187 QRAATRARLVKNLRNAAQALAALQAKQQQEGVVPPQQAGGAMGMASETSGIPPRQHPPLIRGDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLTTGGGGAGRPGPHERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLEPCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVVIDTDQSDLDTPPRWPILEEEEEWDRRA 876
            QRAATRA+LVKNLRNAAQALAALQAKQQQEGV PPQQAGGAMGM SET GIPPRQ PPLI GDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLT G GGAGRPGP ERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLE CVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDV IDTDQS LDTPPRWPILEEEEEWDR A
Sbjct:   53 QRAATRAKLVKNLRNAAQALAALQAKQQQEGVAPPQQAGGAMGMVSETGGIPPRQQPPLIGGDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLTMGEGGAGRPGPRERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLESCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVAIDTDQSALDTPPRWPILEEEEEWDRLA 282

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BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot
Match: A0A7S4M8N6_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4M8N6_9EUKA)

HSP 1 Score: 60.8 bits (146), Expect = 1.650e-7
Identity = 39/162 (24.07%), Postives = 70/162 (43.21%), Query Frame = 1

Query:  361 LIRGDTAEAVALCVAIEQCLFHRIRVKDFGVIPFWAFLERVERLPLTTGGGGAGRPGPHERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLEPCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVVIDTDQSDLDTPPRWPIL 846
            ++  D      LC AIE  L H ++   FG   +W +LE + +                   +   ++ +  +     +S+ LGR RAW+R  L  + L P + +LL E+ L   +Y P AL R  +   ++L L  +L      +  +   L++P  + +L
Sbjct:   35 VLYADNTHVTDLCYAIESLLKHGLQQGFFGTTTYWNYLENIAKC------------------MPDGQSVLQRIRQTPEISSDLGRGRAWVRVALNDQSLGPYLKSLLWEEELTNKYYHPKALMREEDDIALVLMLLESLSHLKFSLTLNDERLNSPDCFELL 178

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BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot
Match: A0A0L0DDL3_THETB (TBC1D2 protein n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0DDL3_THETB)

HSP 1 Score: 57.4 bits (137), Expect = 1.720e-5
Identity = 43/148 (29.05%), Postives = 66/148 (44.59%), Query Frame = 1

Query:  397 CVAIEQCLFHRIRVKDFGVIP-----FWAFLERVERLPLTTGGGGAGRPGPHERRLASVRNTVGAVASLSNVSTPLGRARAWIRQCLVCKCLEPCVAALLEEDRLVKVFYEPTALARCREGSIILLRLCAALEAFDVVIDTDQSDLDT 825
            C A E  + H  + +  G+       FW ++E+V +              PH         T+ ++A++S + +PLGRARAWIR  LV   LE  + ALL    L K +Y   A+ R     + LL   A L + +  +     DLD+
Sbjct:  480 CQAFEAVMRHGFKDRSGGLFSRTPREFWEYVEKVRKYH------------PH------AAPTIKSIAAMSELRSPLGRARAWIRAALVEARLEEYLVALLNHTNLTKEWYADHAVLRSHVDVLALLAQLARLSSINFALCARDVDLDS 609

The following BLAST results are available for this feature:

BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot

BLAST of mRNA_Ecto-sp13_S_contig1331.2652.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 4


Match Name	E-value	Identity	Description
D8LHU4_ECTSI	5.540e-138	93.90	Rap2 interacting protein x isoform 3 n=1 Tax=Ectoc...	[more]
A0A6H5KE74_9PHAE	1.140e-129	94.35	Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA...	[more]
A0A7S4M8N6_9EUKA	1.650e-7	24.07	Hypothetical protein (Fragment) n=1 Tax=Vannella s...	[more]
A0A0L0DDL3_THETB	1.720e-5	29.05	TBC1D2 protein n=1 Tax=Thecamonas trahens ATCC 500...	[more]

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
Ecto-sp13_S_contig1331	contig	Ecto-sp13_S_contig1331:12603..15642 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 vs UniRef90	2022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.0	2022-07-08

Properties


Property Name	Value
Taxonomic scope	Mammalia
Stop	0
Start	0
Seed ortholog score	62.0
Seed ortholog evalue	5.5e-07
Seed eggNOG ortholog	10160.XP_004646598.1
Preferred name	RUFY3
Model size	910
Hectar predicted targeting category	signal anchor
GOs	GO:0001558,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006996,GO:0007010,GO:0007015,GO:0007275,GO:0007399,GO:0008150,GO:0008361,GO:0009987,GO:0010720,GO:0010721,GO:0010769,GO:0010770,GO:0010771,GO:0010975,GO:0010976,GO:0010977,GO:0016043,GO:0022008,GO:0022603,GO:0022604,GO:0030027,GO:0030029,GO:0030036,GO:0030154,GO:0030175,GO:0030307,GO:0030334,GO:0030335,GO:0030424,GO:0030425,GO:0030426,GO:0030427,GO:0030516,GO:0031252,GO:0031344,GO:0031345,GO:0031346,GO:0032386,GO:0032388,GO:0032501,GO:0032502,GO:0032535,GO:0032878,GO:0032879,GO:0032880,GO:0033157,GO:0033267,GO:0036477,GO:0040008,GO:0040012,GO:0040017,GO:0042995,GO:0043005,GO:0043025,GO:0043204,GO:0044297,GO:0044424,GO:0044444,GO:0044463,GO:0044464,GO:0045595,GO:0045596,GO:0045597,GO:0045664,GO:0045665,GO:0045666,GO:0045773,GO:0045927,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048638,GO:0048639,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050768,GO:0050769,GO:0050770,GO:0050771,GO:0050772,GO:0050789,GO:0050793,GO:0050794,GO:0051049,GO:0051050,GO:0051093,GO:0051094,GO:0051128,GO:0051129,GO:0051130,GO:0051222,GO:0051223,GO:0051239,GO:0051240,GO:0051241,GO:0051270,GO:0051272,GO:0051960,GO:0051961,GO:0051962,GO:0060284,GO:0060341,GO:0061387,GO:0065007,GO:0065008,GO:0070201,GO:0071437,GO:0071840,GO:0090066,GO:0090087,GO:0090316,GO:0097435,GO:0097447,GO:0097458,GO:0098858,GO:0120025,GO:0120035,GO:0120038,GO:0150034,GO:1903827,GO:1903829,GO:1904951,GO:2000026,GO:2000114,GO:2000145,GO:2000147
Exons	4
EggNOG free text desc.	RUN
EggNOG OGs	35M3G@314146,38BTR@33154,3B966@33208,3CRQ7@33213,3J8UH@40674,4821C@7711,48Z0T@7742,4Q7XZ@9989,KOG4381@1,KOG4381@2759
Cds size	909
COG Functional cat.	Z
Best tax level	Rodentia
Best eggNOG OG	NA\|NA\|NA

Relationships

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1681462711.395724-CDS-Ecto-sp13_S_contig1331:12602..12791	1681462711.395724-CDS-Ecto-sp13_S_contig1331:12602..12791	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig1331 12603..12791 +
1681462711.4056191-CDS-Ecto-sp13_S_contig1331:13655..13914	1681462711.4056191-CDS-Ecto-sp13_S_contig1331:13655..13914	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig1331 13656..13914 +
1681462711.4157524-CDS-Ecto-sp13_S_contig1331:14660..14914	1681462711.4157524-CDS-Ecto-sp13_S_contig1331:14660..14914	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig1331 14661..14914 +
1681462711.4262369-CDS-Ecto-sp13_S_contig1331:15435..15642	1681462711.4262369-CDS-Ecto-sp13_S_contig1331:15435..15642	Ectocarpus species13 EcNAP12_S_4_19m	CDS	Ecto-sp13_S_contig1331 15436..15642 +

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_Ecto-sp13_S_contig1331.2652.1	prot_Ecto-sp13_S_contig1331.2652.1	Ectocarpus species13 EcNAP12_S_4_19m	polypeptide	Ecto-sp13_S_contig1331 12603..15642 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at Ecto-sp13_S_contig1331:12603..15642+
coding sequence from alignment at Ecto-sp13_S_contig1331:12603..15642+

protein sequence of mRNA_Ecto-sp13_S_contig1331.2652.1

>prot_Ecto-sp13_S_contig1331.2652.1 ID=prot_Ecto-sp13_S_contig1331.2652.1|Name=mRNA_Ecto-sp13_S_contig1331.2652.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=303bp
ACTTRQLVRCPLHSVLCTLYPVFCTLWLVVVEPPAVQTMSWLSSIISGPQ
DSRESGKQVEARQRAATRARLVKNLRNAAQALAALQAKQQQEGVVPPQQA
GGAMGMASETSGIPPRQHPPLIRGDTAEAVALCVAIEQCLFHRIRVKDFG
VIPFWAFLERVERLPLTTGGGGAGRPGPHERRLASVRNTVGAVASLSNVS
TPLGRARAWIRQCLVCKCLEPCVAALLEEDRLVKVFYEPTALARCREGSI
ILLRLCAALEAFDVVIDTDQSDLDTPPRWPILEEEEEWDRRAEATYSAAA
AAD

mRNA from alignment at Ecto-sp13_S_contig1331:12603..15642+

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_Ecto-sp13_S_contig1331.2652.1 ID=mRNA_Ecto-sp13_S_contig1331.2652.1|Name=mRNA_Ecto-sp13_S_contig1331.2652.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=3040bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1331:12603..15642+ (Ectocarpus species13 EcNAP12_S_4_19m)
GCTTGCACCACGAGACAGTTGGTCCGCTGCCCTCTGCACTCTGTGCTCTG
TACTCTGTACCCTGTATTCTGTACTCTGTGGCTGGTCGTGGTGGAGCCGC
CAGCGGTGCAAACGATGAGCTGGCTGTCTTCCATCATCTCTGGTCCTCAG
GACAGCCGCGAAAGCGGCAAGCAGGTGGAAGCTAGACAGGTAAGGACACG
ATTGCTGCTGTTGTTGTGCCTGGGCTCTCTTGCTTCGTCTCACGGCCTTC
TTTGACTACCGAGCCGCTGCGGTGCGCTGCGAAGTCTGCTGGCTTGCCGC
TTTTCACATCCGCACTGGCAACAATGGAAAGCGTTGGCACCAAGTTGTCT
CAGTTGGAGTACTTACGCATTTTCTGGCACGTTTCTTTCATGGACAATGA
TGCGATAGTCATTACTGTAAGGCCGGGACATCACCGCTGTAGTTAAAGAT
TTTAATATCTCCAACTGCCGTCTATCCTGTGCACTGTATTGTCGTATGCA
TACCAACCAACACGTCACGGGTTCTGCGATACCTAAGCCGACCACATGCA
GTCTCTCCGCCGGCTACTTGGGCAATACGCTTTCGCATGACAGAGTAAAC
CTTTTTGCTGAAAGGTACTACGCACATAAACCTACGACCTAAAGAGGCGC
CCTGACACAATCCCACCGCATTTGCACACTGCACCCACAACCCACAACAC
CTTCGAGACACTCTGCCCACAGCAGTCTGCATTTATGCACACCTGTACTT
GAATTGTTTCAAAACTCGCATCTTTGCAAATACTTTCGTCGGCACCAATG
GATTGTGTCGGTCGTAGCGTCTCTCGACGACTGTACATAGGTGCAAACAC
TCTATTGGACGGCATGCACAAATCAATGTCTTCCTTGTTCCTTGAATACT
GGAGAATGTAACCAACTTCAACCCCGCCGACACAACTCAACGCAAACTAT
ACCAACACGACACGCTCGACGCCCCGCTTCCCACCATACTCCCACCAAAC
TGAGTTTTCTAAAATATCGTCTCCCCCGGCACCACCGAACATTCCTTCTT
CAGCGAGCTGCCACTAGAGCGAGGCTTGTGAAGAACCTTAGAAATGCCGC
ACAAGCCTTGGCGGCGCTGCAAGCCAAGCAGCAGCAGGAAGGGGTTGTGC
CGCCGCAGCAGGCCGGCGGCGCCATGGGCATGGCCTCCGAAACGAGCGGG
ATTCCGCCGCGTCAACATCCGCCGTTGATTAGAGGAGACACTGCCGAGGC
TGTCGCGTTGTGCGTCGCCATCGAGCAGTGCCTGTTCCATCGCATCCGGG
TTAAGGACTTCGGTGAGAGAGGCACTACCACCACAGTAGAGGAAGAAGAG
GCCACCCCTAGTCTTTTGGTCGCGAGATGTTTGGCGTTACTGAAGTATGT
ACGAGAGTTTGGTTGGATCGATGGTTAATGTTGGTGTGCGGGTCTCGGTT
TTGTCGGGATCTCCCACACCTGCGACATGGAGACAAAGCCTCCGAGTGTA
GCCTTTGCACGTAAGCTACTACATTGACACTGCTTGTTTTTGTGCTAGGC
TGGCGGTCTTTTCGTGTTGATCTGTTTGTTGAATGGTATTCACTTCGTGG
CAAGGGAGATGGGGTGGGGGGAGTAGAGGATATCTCAGGGTGGTTGATCA
TCTGGCTCCCCGGAGTTGCCCCCTCGTGTCCTGCCGTTACAACACAGCGT
AGCCTATCAGGTATCTTGAAACCCCCCGCTTGTTTCACTTTCATGATTCG
ATGCCAGGTCGAGCTTCGGCGCGCCCCTCTCGTCTCTTACGTGATTTTTC
ACGTGCGTGGTGAATTTGCGTCCACCGGGTCTCACCGCGCTCGCTACATG
CTACCTCATCGCTTCCCGGGCATCGTGGGCATCGTCAACACGTTGAAAGA
GAGATACCCGTTGTAGCCTCTGCCAACGTGGCTCTCTTGCCGGCAATCCC
CCCACCCCCACGCACGAACCAATATCTAGATACCAGTGCCCCACCCACCC
CTGTACCTTGCCCCTTTTTCCAAACCGCCGTGCCCCCCACTACCTATTCC
GGTCCCAGGCGTGATACCCTTTTGGGCCTTCCTTGAGCGGGTGGAGCGGC
TCCCCCTTACGACGGGGGGGGGTGGGGCCGGCCGTCCGGGTCCGCACGAG
CGCCGTCTTGCGAGCGTCCGGAACACGGTCGGGGCCGTGGCCTCCCTGTC
GAACGTTTCGACGCCGCTGGGGAGGGCGAGGGCGTGGATCCGCCAGTGCC
TGGTGTGCAAGTGCCTGGAGCCTTGCGTGGCGGCGTTGCTGGAGGAAGAC
CGGCTCGTGAAGGTGTGTAGGTCGGTTGTCGTTGGGGAGCGTTTCTTTTT
CGGCTTGCCCTGCCCCGTAGAGCTACATTGCCTGCCCGATGCTTGGGGCT
GCGGTGTTTATGCCGGTTTGGGAAGCAGGCTTCATTGCCGACGACACAAA
GCATGCGCGTGATTGGGATCCGACTTGAACCTCTCGAAACTGCTTGCGTC
CTCCAAGAAATGCCGAACCCGGGGCAATCCCCAAGCGAATTGTTTTGCTG
GCACGCCTGCTGTGCGAGATAGACGGCTGTCCGTACGGTGTCCTCAACGC
CCCCTCCGTCCCCCCCTCTGCAGTAGCCTTCACAAGGATGATTATGTTGG
TTAAAGTAGGGGGTACGGGATTGGGTTAAGGCTGCGAACCCCCCTCTCTA
AATCATTGACTAAAAATATTACCGTTGCGAGGGCGGTCCCCCCCCCGGCT
AACCCCGGTCCAGCCGTGAGTTGTGAGTTTCTCTTCTCCCTGCACCCTCT
TCCGTCCGCGCACCCGTTCCCTGTCAAAATAAGGTGTTCTACGAGCCGAC
AGCCCTGGCACGATGCCGAGAAGGATCGATCATCCTCCTGCGCCTGTGCG
CGGCCCTCGAAGCATTCGACGTCGTAATCGACACCGACCAATCGGATCTC
GACACCCCGCCGCGCTGGCCAATCCTCGAAGAGGAAGAAGAGTGGGACCG
ACGCGCGGAAGCAACGTATTCAGCGGCAGCCGCCGCCGAC

Coding sequence (CDS) from alignment at Ecto-sp13_S_contig1331:12603..15642+

>mRNA_Ecto-sp13_S_contig1331.2652.1 ID=mRNA_Ecto-sp13_S_contig1331.2652.1|Name=mRNA_Ecto-sp13_S_contig1331.2652.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=909bp|location=Sequence derived from alignment at Ecto-sp13_S_contig1331:12603..15642+ (Ectocarpus species13 EcNAP12_S_4_19m)
GCTTGCACCACGAGACAGTTGGTCCGCTGCCCTCTGCACTCTGTGCTCTG
TACTCTGTACCCTGTATTCTGTACTCTGTGGCTGGTCGTGGTGGAGCCGC
CAGCGGTGCAAACGATGAGCTGGCTGTCTTCCATCATCTCTGGTCCTCAG
GACAGCCGCGAAAGCGGCAAGCAGGTGGAAGCTAGACAGCGAGCTGCCAC
TAGAGCGAGGCTTGTGAAGAACCTTAGAAATGCCGCACAAGCCTTGGCGG
CGCTGCAAGCCAAGCAGCAGCAGGAAGGGGTTGTGCCGCCGCAGCAGGCC
GGCGGCGCCATGGGCATGGCCTCCGAAACGAGCGGGATTCCGCCGCGTCA
ACATCCGCCGTTGATTAGAGGAGACACTGCCGAGGCTGTCGCGTTGTGCG
TCGCCATCGAGCAGTGCCTGTTCCATCGCATCCGGGTTAAGGACTTCGGC
GTGATACCCTTTTGGGCCTTCCTTGAGCGGGTGGAGCGGCTCCCCCTTAC
GACGGGGGGGGGTGGGGCCGGCCGTCCGGGTCCGCACGAGCGCCGTCTTG
CGAGCGTCCGGAACACGGTCGGGGCCGTGGCCTCCCTGTCGAACGTTTCG
ACGCCGCTGGGGAGGGCGAGGGCGTGGATCCGCCAGTGCCTGGTGTGCAA
GTGCCTGGAGCCTTGCGTGGCGGCGTTGCTGGAGGAAGACCGGCTCGTGA
AGGTGTTCTACGAGCCGACAGCCCTGGCACGATGCCGAGAAGGATCGATC
ATCCTCCTGCGCCTGTGCGCGGCCCTCGAAGCATTCGACGTCGTAATCGA
CACCGACCAATCGGATCTCGACACCCCGCCGCGCTGGCCAATCCTCGAAG
AGGAAGAAGAGTGGGACCGACGCGCGGAAGCAACGTATTCAGCGGCAGCC
GCCGCCGAC