mRNA_Ecto-sp13_S_contig1258.2133.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 1906 bits (4937), Expect = 0.000e+0
Identity = 979/1100 (89.00%), Postives = 998/1100 (90.73%), Query Frame = 2

Query:  173 SARTWAGASAGWSSPGKSLPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQDETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEE---GRDRLSARHGVRMRMSNVEGLSIELTPGTLASRSAEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEGGDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSS-FSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDGFAETADTSETGLSTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDGDTEAPVDAESGQSGAPTAMSAWSCLCVSLVYGVLAPLSAIALATLS 3460
            +ARTWAGAS GW  PGKSLPYPSLELVENALSTTAK DFNGHARAFEAAATWNGERLRH QDETRAPHLCC +YGHGRETFSRLKQFLSPEAVRVVSHSE  GACFFASASHAQAS I+EDQEQFGLESFSPFPS LKLAPGLVDHSQ HDKD+E   G DRLSARHG RMRMSNVEGLSIELTPGTLASRS EAEAFISDTLGDLM ASVDLHSTNFWS Q + GG+QLSFPGGAVRARDWSKAASVVHELSE+AGTSPG+ICSWNRVSVHHAEGDVLLVSGLDHLL SGRGAGGRSDG E+AELHIACFMGLVSFLASKPEVLRVAPRQSQALLNA+ARANIQSATVT+TPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSS FS+PTSDDDDS GWP AT DH TGSYYQIAEEFDGGDFTI+PDRRKIIQYINLIKSDDTEGSHGD FTTSLGQQYPWLPAD FEYDYEAGHGTHVAGSVAGATLNTPAELISC DTETMSCVGACVDDDEANDVDDLATP VEI DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYD+FGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGE RRSTCTM SPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAY GTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSS TLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPL GEGSL LFVSDSNYTSIPERTRQEYNFDVNATA LDLRVTLSWLDPPATTFSAVQLVHDLDL+VISPNGTRYTMW SGVTDS+NVNERVIVDAGDVETGTWSVWI ADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDG  ET    ETGLST                                                 L  DTEAPVDAESGQ+GA TA+SAWS LCV L+YGVLAPLSAIALATLS
Sbjct:    3 TARTWAGASVGW--PGKSLPYPSLELVENALSTTAKDDFNGHARAFEAAATWNGERLRHRQDETRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDRLSARHGARMRMSNVEGLSIELTPGTLASRSVEAEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLPADRFEYDYEAGHGTHVAGSVAGATLNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERRRSTCTMSSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDGVLETPPRWETGLSTSDSDTADTYTEPEEITSSD------------------------------LYWDTEAPVDAESGQNGARTAVSAWSSLCVWLMYGVLAPLSAIALATLS 1070          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: D8LNG4_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG4_ECTSI)

HSP 1 Score: 1321 bits (3420), Expect = 0.000e+0
Identity = 690/790 (87.34%), Postives = 703/790 (88.99%), Query Frame = 2

Query: 1097 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSS-FSAPTSDDDDSWGW-PWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDGFAETADTSETGLSTXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLDGDTEAPVDAESGQSGAPTAMSAWSCLCVSLVYGVLAPLSAIALATLS 3460
            MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVT+TPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINP LTSS FS+P           PWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGD FTTSLGQQYPWL ADHFEYD EAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCD LET+KCLSDDVAETLTNNGGMAQGAKLAIFDAFYD+FGLSPFVG GLWEPCA+ADCKIHSNSYGGDFECSLG NDVLYD WMVSNPENLLIFAAGNDGELRRSTCT+GSPAIAKNVLAVGASTSGDTRLSATPG GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGS+D+HSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSS TLKALMINSANLMG SSEP+GSRGFGRIHLEAGMPLGGEGSL LFVSDSNYTSIPE TRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDL+VISPNGTRYTMW SGVTDSSNVNERVIVDA DVETGTWSVWI ADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDG AETA   ETGLST                                                 L  DTEAPV+AESG SGAPTA+SAWS LCVSL+ GVLAPLSAIALA LS
Sbjct:    1 MGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPSLTSSSFSSPXXXXXXXXXXXPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDSFTTSLGQQYPWLSADHFEYDDEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDGLETDKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDTFGLSPFVGTGLWEPCADADCKIHSNSYGGDFECSLGSNDVLYDEWMVSNPENLLIFAAGNDGELRRSTCTIGSPAIAKNVLAVGASTSGDTRLSATPGSGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSVDTHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSATLKALMINSANLMGESSEPNGSRGFGRIHLEAGMPLGGEGSLALFVSDSNYTSIPELTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSSNVNERVIVDADDVETGTWSVWIWADNLTTDVQSYSLVVNGAISPGTGVGAIERSSSTSSTSSDGLAETAPPWETGLSTSDSDTADTYTAPEEISSSD------------------------------LYWDTEAPVEAESG-SGAPTAVSAWSSLCVSLMSGVLAPLSAIALAALS 759          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 1204 bits (3115), Expect = 0.000e+0
Identity = 641/978 (65.54%), Postives = 749/978 (76.58%), Query Frame = 2

Query:  185 WAGASAG----WSSPGKSLPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQDETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEE---GRDRLSARHGVRMRMSNVEGLSIELTPGTLASRSAEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEGGDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDY--FTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIA--DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPG-LGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAISPGTGVGA 3082
            WA A A     WS P       SLELVEN L+TTAK DFNGHARAFEAAATWNGERLR SQD TRAPHLCC +YGHGRETFSRLKQFLSP+AVRVVSHSE HGACF A+ASHAQAS IMEDQEQFGLESFSPFPS LKLAPGLVDHS+S + + E   G+DRL ARHG  MRMSNVEGL+++LTP TL +RSA AE+FI D LGDLMSASVDLHSTNFWS   + GG+ LS  GGAVRARDWSKAASVVHELSE+AGTSPG+ICSW+ V++  A  D LLV+GLDHLL SGRGAGG  +  E+ ELH+ACFMGLVSF A +PEVLRV+PR ++ +LN AAR  IQSA+ T+TPLTDAGLDGTG+V+QV+D+G+DE+SCFF  D+ G+ V HG++FEE  +    +S                          YY +   F+ GDFT   +RRK+IQYI L+  D       DY  F+T+ G  YP+LP D F  D  AGHGTH AGS AGATLN PAE ++C+  ET+ CVG C+D+ E  D  DL +  ++IA  DLDRLCP+   Y CD   T+ CLSD+V ETLT NGGMAQGAKL+IFD F++ FGL   +GNG+WEPC EA CK+HS S GGD  C+ G +++LYD +M  NPENLLIFAAGNDGE  RS CTM SPAI KNVLAVGASTSGDTR+ +T G  GID VA FSSYG T+D RIKPEV+APGD +YSAASDG+ DSHSCRLWAY GTSMS PIVAGA+AM+RQYF++ETF+  D+TARGFC +   CE FSPS+ T+KAL+INSANLMG SS+PD  RGFGRIHLE GMPL GEG L LFV+D+  TSI E T QEY FDV+A AGL+LR TLSW+D PAT+ SA+QLV+DLDL+V+SP+GT Y MW S VTD+ NVNERVIVDA DVETGTW+V + +++L TD QSYSLVVNGAIS GTG GA
Sbjct:   18 WAPARAAAADTWSPP-------SLELVENGLATTAKDDFNGHARAFEAAATWNGERLRRSQDGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHSESREVEGEPSSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAGAESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHE-EESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFI-DDGGEEVDHGYFFEELFVGSTGSSQLI-----------------------YYPV---FEAGDFTYTEERRKVIQYIELVNPDS------DYVAFSTTQGGVYPFLPPDEFGQDDSAGHGTHTAGSAAGATLNDPAETLTCSGDETLGCVGGCIDESETTD--DLVSYAIQIALPDLDRLCPM---YGCDG-STDPCLSDEVDETLTKNGGMAQGAKLSIFDVFFEVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETERSVCTMASPAIGKNVLAVGASTSGDTRVISTEGGAGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGT-DSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAISAGTGEGA 947          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 1186 bits (3068), Expect = 0.000e+0
Identity = 633/977 (64.79%), Postives = 735/977 (75.23%), Query Frame = 2

Query:  227 LPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQDETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEE-----GRDRLSARHGVRMRMSNVEGLSIELTPGTLASRSAEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEG-GDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGL-GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAISPGTGVGA-----IERSSSTSSTSSD 3121
            L  P+LELVENAL TTAK DF GH  AFEAAATWNGERLRHSQ+ETRAPHL C +YG G E  SRLKQFLSPEAVRVVSHSE HGACFFASASHAQA+ I+EDQEQFGLE+FSPFPSPLKLAPGLVDH++SHD+ EE     G DRLSARHG RMR  NVEGLS+ELTPGTLA+RS+EA++FI+  LGDLMSASVDLHSTNFWS   ++G G+ LS P GAVRARDW KAASVVHELSE+A T PG+ICSWNR+ VHHA  D LLVSGLDHLL SGRGAGG  +  E+ ELH+ACFMGLVSFLA +PEVLRV+PR ++ +LNAAAR  IQSA+ T+TPLTDAGLDGTG+++QVIDSG+DE+SC+F  D+SG+ V HG+YF+E G     +SS+S+                        + A  F+GGDF+ YP+RRK                                          AGHGTH AGS AGATLN PAE I+CN T+T+SCVGAC+D  +A D     +   +  DLDRLCP+   Y CD      CLSDDV ETL+NNGGMAQGAKLAIFD FY  +  S  +GN LWE C EA CK+HSNS G DFECS+   D+L D +M  N ENLLIFAAGN+G + RSTCT+ +P I KN LA+GASTSGDTR+ +T G  GIDTVA FSS+G T+D RIKPEV+APGD++YSAASDG+ D+HSCRLWAY GTSMS PIVAGA+AMIRQYF++ETF+  D+TARGFC +   CE FSPS+ T+KAL+INSANLMGGSSEPDG RGFGR+HLEAGMPL GEG LVLFV+D+  TSI   T +EY FDV+A AGLDLR TLSW+DPPAT  SAVQLVH+LDL+V+SP+GT Y MW S  TD+ NVNERVIVDA DV TGTW+VW+ A+NL TD QSYSLVVNGAISPGTG GA      E SSS +S SS+
Sbjct:   24 LNSPALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEETRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDRLSARHGARMRKPNVEGLSVELTPGTLAARSSEAKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHE-EESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELGRAAVYSSSYSSD-----------------------ETATIFNGGDFSYYPERRK------------------------------------------AGHGTHTAGSAAGATLNDPAETITCNVTDTLSCVGACIDLLDATDDLVSISSQTDGTDLDRLCPM---YGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFCSDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAISPGTGEGASGSSSFEVSSSATSASSE 929          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 934 bits (2414), Expect = 0.000e+0
Identity = 516/955 (54.03%), Postives = 629/955 (65.86%), Query Frame = 2

Query:  188 AGASAGWSSPGKSLPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQ-DETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEEGRDRLSARHGVRMRMSNVEGLSIELTPGTLASRSAEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEGGDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAEL-ISCNDTETMSCVGACVDDDEANDVDDLATPYVEIA----DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELR--RSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG-CEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWV-SGVTDSSNVNERVIVDAGDVETGTWS 2989
            A AS  W     +LPYPSL +VE +L TTA+  F+GHA+AFE AA W GE LR  + D  RAPHL CT+Y  G +T  RL++ LSP AVR V+HS  HGACFF +ASHA+A   M D   +GL S SPFPS LK+APG+++H  S D+  +   RL+  HG  MR  NVEGL++EL+PGTL + S EA  FI++ L DLMS S+DLH  N WS      G+ L+ P GAVR R+WS AA++VH+L+ +  TSPG+ICSW+ +S+H+A  D+LLVSGLDHLL    GA G  +  EA EL +ACFMGLVSFL+++ EVLRV+PR +  LLNA AR  IQSAT+T  PLTDAGLDGTGQV+QVID+G+DE+SCFF  D+ G+ VPHGHY+E  G   P +SS                                                        DD EG                             HGTH AGS  GAT+ TPAE   +C+ TE   CVG C+D D  +  DDL T   E +    D+DRLCP+    DCD     +CLSDDV +TL ++GGMAQGAKLA FD F D    + FVGNGLWEPC EA CKIHS S G DFECS+   DVLYD +M +NPENL++ AAGN G+    R+ CTM SPAI KN LAVGA++SG+TR++ T   G           +DTVA FSSYGPT D RIKPEV+APGDAIYSA SDGS D+HSCRL A  GTSM+ PIVAGASAM+RQYFM+E+F+  D  ARG+ D+    CE+FSPSS T+KAL+INSANLMGGSSEPDG RGFGRIHLE GMPL GEGSL LFV+DS+YTS+PE   Q++ FDV+A A LD R TLSW+DPPA++ S VQLVHDLDL V+SP+G  + MW  SG  DS NVNERVI+ A DVE+GT++
Sbjct:   16 AAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEA-RAMSDSPYYGLTSISPFPSALKVAPGVLEHGVSLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPEAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDE--EATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFI-DDDGEEVPHGHYYEALGEIIPSSSS------------------------------------------------------ARDDVEG-----------------------------HGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMI---DCDDATDPRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFVGNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTEDGRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGS-DNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGWSDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 930 bits (2403), Expect = 0.000e+0
Identity = 512/976 (52.46%), Postives = 632/976 (64.75%), Query Frame = 2

Query:  194 ASAGWSSPGKSLPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQ-DETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEEGRDRLSARHGVRMRMSNVEGLSIELTPGTLASRSAEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEGGDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAEL-ISCNDTETMSCVGACVDDDEANDVDDLATPYVE----IADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELR--RSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG-CEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWV-SGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAI 3058
            AS  W     SLPYPSL +VE +L TTA   F+GHA+ FE AA W G+ +R  + D  RAPHL CT+Y  G ET SRL++ LSPEAVR V+HS  HGACFF +ASHA+A   M D   +GL S SPFPS LK+APG+++H  S D+  +   RL+  HG  MR  NVEGL++EL+PGTL + S +A  FI++ L DLMS S+DLH  N WS      G+ L+ P GAVR R+WS AA++VH+L+ +  TSPG+ICSW+ +S+HHA  D+LLVSGLDHLL    GA G  +  EA EL +ACFMGLVSFL+S+ EV RV+PR +  LLNA AR  IQSAT+T  PLT AGLDGTGQV+QVI                                                                                      QYINLIK+D   G      TTS G +  W+  D F+ D    HGTH AGS  GAT+ TPAE   +C+ TE   CVG C+D D  +  DDL T   E    +AD+DR+CP+     CD      CLSD V +TL ++GGMAQGAKL+ FD +  +     F+GNGLWEPC EA CKIHS S+G D EC++   D+LYD +M +NPENL++ AAGN GE+   R+ CTM SPAI KN LAVGA++SG+TR+  T   G           +DTVAVFSSYGPT D RIKPEV+APGDA+YSA SDGS D HSCRL A  GTSM+ PIVAGASAM+RQYFM+E+F+  D  ARG+CD+    CE+FSPSS T+KAL+INSANLMGGSSEPDG RGFGRIHLE GMPL GEGSL LFV+DS+YTS+PE + Q++ FDV+A A LD R TLSW+DPPA++ S VQLVHDLDL V+SP+G  + MW  SG  DS NVNERVI+ A +VE+GT++V +  ++L TD Q+YSLVVNGA+
Sbjct:   18 ASTPWGGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEA-RAMSDNPYYGLTSISPFPSALKVAPGVLEHGVSRDQASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQAGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDE--EATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI--------------------------------------------------------------------------------------QYINLIKADSVSGPASS--TTSGGNRVWWVSPDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLG---CDEENEPWCLSDGVEQTLADHGGMAQGAKLSFFDIYDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIEDGRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSDVDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGS-DVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTSLPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAM 898          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 803 bits (2074), Expect = 1.170e-267
Identity = 469/976 (48.05%), Postives = 613/976 (62.81%), Query Frame = 2

Query:  236 PSLELVENALSTTAKGDFNGHARAFEAAATWN-GERLRHSQDETRAPHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEEGRD-----------RLSARHGVRMRMSNVEGLSIELTPGTLASRS---AEAEAFISDTLGDLMSASVDLHSTNFWSYQKVEGGDQLSFPGGAVRARDWSKAASVVHELSESAGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELISCND-TETMSCVGACVDDDEANDVDDLATPYVEIADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDG-ELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATP--GLG---------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVE----TGTWSVWILADNLTT-DVQSYSLVVNGAISP 3064
            PSL   E  L TTA  DF+  A AF  A+ W  G+R   S     +P+L C ++G GR  + +L+ FL   AV+ VS +  HGACF  +AS A+A+ +   ++     +F  FP+ LK+APGL+DH  S D     RD           RLS  HG  M++ +  GL +EL+PGTL S S   A+A  FI + L  L S S+DLH+   WS   ++GGD L    GA+R R+WS+AA++V+E SES   +P ++C W +V VHHA  DVLLV+GLDHLL  GR   G S+  EAAEL +AC+MGLVSFLAS+ EVL+VA    +  LNAAARA IQSA+V +TPLTDAGLDG+G+V+Q+ID+GV+E++CF  H + G  V HG+YF+    + PL +S S   S                         EF GGDF+   DRRK+IQYI+LI  D  +GS       S G   P++ AD F  D + GHGTH A +VAG+T+++PAEL+SC D +E + C G C+ +      +     Y E  D++RLCP F          ++CL DD A+TL+ +GGMAQGAK+A  D FY  +      GNGLWE CA+A CKIHSNSYG D  C L   DVLYD +M  N E+LL+F+AGN G    R TCT+ SP IAKNVL VG+++SG TRLS T   G G         ID V+ FS YG T D RIKPEV+APGD IYSA+ +G  DS SC L AY GTS ST + +GA+AM+RQYF + +F++ D+ +RGFCD+   C++FSP + T+KA++INSAN+MGG +EPD  RGFGRIHLEAGMPLGG G + LFV+DS+   +    + ++ F+V+  AG++LR TLSW DP A++ S  QLV+DLDL V +P+GTRY MW     D+SNVNERVIV A  V     T  W V +   + T  D  S+SLVV GAISP
Sbjct:   25 PSLGFFEQTLLTTAGADFDSEA-AFAFASAWQAGDRQGRS-----SPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEAAQLSSWED---FTTFGAFPAALKIAPGLLDHG-SCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASE-EEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGD-GTEVEHGYYFDFVASSNPLFTSSSESRS---------------------MFKAEFTGGDFSYDSDRRKVIQYIDLINPDTGDGS-------SSGM--PFVSADSFVSDDDLGHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSE---ADYGE--DINRLCPAFG------CSGDQCLPDDTADTLSEHGGMAQGAKIAAIDVFYGDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTDRDTCTIDSPGIAKNVLTVGSTSSGVTRLSTTDLDGRGYADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGG-DS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQGFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAISP 945          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A6H5K365_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K365_9PHAE)

HSP 1 Score: 788 bits (2034), Expect = 1.030e-265
Identity = 436/729 (59.81%), Postives = 526/729 (72.15%), Query Frame = 2

Query:  998 LLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPW-LPADHFEYDYEAGHG--------THVAGSVAGATLNTPAELISCNDTETMSCVGACVDDDEANDVDDLATPYVEIA--DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGL-GIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAISPGTGVGAIERSS------STSSTSSDGFA 3130
            +  +GLDHLL SGRGAGG  +  E+ ELH+ACFMGLVS LA +PEVLRV+PR ++ +LNAAAR  +QSA+ T+TPLTDAGLDGTG+++QVIDSG+DE+SC+F  D+SG+ V HG+YF+E       +S +S+                        + A  F+GGDF+ YP+RRKIIQYI+L+  D    S    F TS G    + LP D F  D EA             V  S AGATLN PAE I+CN T+T+SCVGAC+D    +  DDL +   +    DLDRLCP+   Y CD      CLSDDV ETL+NNGGMAQGAKLAIFD FY  +  S  +GN LWE C EA CK+HSNS G DFECS+   D+L D +M  N E+LLIFAAGN+G   RSTCT+ +P I KN LA+GASTSGDTR+ +T G  GI+TVA FSS+G T+D RIKPEV+APGD++YSAASDG+ D+HSCRLWAY GTSMS PIVAGA+AMIRQYFM+ETF+  D+TARGFC +   CE FSPS+ T+KAL+INSANLMGGSSEPDG RGFGR+HLEAGMPL GEG+LVLFV+D+  TSI E T  EY FDV+A AGLDLR TLSW+DPP+T  SAVQLVH+LDL+V+SP+GT Y MW S  TD+ NVNERVIVDA DV TGTW+VW+ A++L TD QSYSLVVNGAISPGTG GA   SS      +TS++S DG A
Sbjct:    1 MHATGLDHLLFSGRGAGGSHE-EESTELHVACFMGLVSVLAGRPEVLRVSPRPTKRVLNAAARGIVQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFI-DDSGEEVDHGYYFDELAQAAVYSSLYSSD-----------------------ETATIFNGGDFSYYPERRKIIQYIDLVNPDSPYSS----FYTSQGSYIGYFLPPDQFGEDDEARVALRKKDPSQARVGRSAAGATLNNPAETITCNVTDTLSCVGACID--LLDVTDDLVSISHQTGGTDLDRLCPM---YGCDNSTDTTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGDYDASSSIGNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAEHLLIFAAGNEGGEERSTCTINTPGIGKNSLAIGASTSGDTRVISTVGATGINTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGT-DTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFMDETFYMADVTARGFCSDVFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGNLVLFVADALDTSIAEGTLDEYLFDVDADAGLDLRATLSWIDPPSTATSAVQLVHNLDLVVVSPDGTTYRMWGSETTDNRNVNERVIVDATDVVTGTWTVWVWANSLITDSQSYSLVVNGAISPGTGEGASGSSSFEVSSLATSASSEDGTA 694          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A6H5JKA8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JKA8_9PHAE)

HSP 1 Score: 747 bits (1929), Expect = 2.960e-244
Identity = 476/1092 (43.59%), Postives = 613/1092 (56.14%), Query Frame = 2

Query:  197 SAGWSSPGKSLPYPSLELVENALSTTAKGDFNGHARAFEAAATWNGERLRHSQDETR-APHLCCTQYGHGRETFSRLKQFLSPEAVRVVSHSEHHGACFFASASHAQASTIMEDQEQFGLESFSPFPSPLKLAPGLVDHSQSHDKDEEGRDRLSARHGVRMRM--------------------------------------------SNVEGLSIELTPGTLASRS----------AEAEAFISDTLGDLMSASVDLHSTNFWSY---------QKVEG-GDQLSFPGGAVRARDWSKAASVVHELSES-AGTSPGEICSWNRVSVHHAEGDVLLVSGLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEG--------------------------SHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAELIS------CNDTETMSCVGACVDDDEANDVDDLATPY--------VEIADLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLS-PFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELRRSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLG-----------IDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAGCEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWVSGVTDSSNVNERVIVDAGDV---------------ETGTWSVWILADNLT-TDVQSYSLVVNGAISPGT 3070
            + G +S   +LP+P L ++  AL TTA   F  H   F+AA  W+  RL  ++     A     +  G    T S +         RVV  S  HG C  A+A+  QA +I + Q+ F   +  P    L        H        +GR R++    +R                                               S V GL++EL PG LA+ +          A +     +   +LMS S+D+H  +FWS             EG GD      G V AR+W++AA V+  L  +  G + G++CSW+ + V H + D+L + G+DHLLP GRGAGG  +GPEA EL +ACFM L+SFLAS+PEVLRV+P ++  LLNAAA  N+QSA++T +P+TDAGLDGTG+V+Q++D+G+D +SCFF  ++ G+ V HG+YFEE G+     SSF         SW   W   D +     QIA  F GGDF     RRK +       S D+                            ++G+ + TS G+QYPW+ A  F  D   GHGTH AGS AGA +++P+   +      C +   +SCVG C+D D+    DDL T +         E  DLDRLCP     DCD L  E CL DD AETL  +GG+A+GA+LAIFD   D  G+     GNG+WEP  EA  K+ SNS+G    C     DVL+D +M  N + LL+FAAGNDG+   S CTMG+PA+AKNVLAVGASTSG  RLS T   G           ID +A FSS+GPT D+RIKPEV+APGD IYSAA  G   +++CRLW Y GTSMSTPIVAGA+A++RQYF NETF+A D+  RGFCD + GCE F+PS+ T+KA++INSANLMGGS+EPDG RGFGR+HLEAGMPL GEG   LFV+DS  TS  E T  EY+F V+A AG+DLR TLSW+DPPATTFS+VQLVHDLDL V SP G RY+MW  G  DS N NERVIV A ++                 GTW+V + +  L   + QSYSLVV GAI P T
Sbjct:   17 AGGGASAAPTLPHPPLSVIRGALLTTAGVTFR-HQGEFKAAVEWHEARLDDARPAPYLACASPDSDRGGSGATLSHVLG-AGAIGGRVVVSSAEHGVCVVATATPGQAESIFQGQQVFSSWAAFPSALKLAPGLLDHPHDDRDGGGGDGRLRMTHGERLRRPKDRKGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDNIGGGPSGVTGLTVELAPGVLAATNRRGSSGGGWYASSGELTDEWREELMSQSLDVHDKSFWSSGWSAKLPDEAAAEGAGDSSGGGPGEVLAREWTRAADVLRGLGGTEGGPTIGDVCSWDGLVVVHTDDDLLTMKGMDHLLPEGRGAGGN-EGPEADELKVACFMALLSFLASRPEVLRVSPARAIKLLNAAAYHNVQSASLTESPMTDAGLDGTGEVIQIVDTGLDVNSCFFVDEDGGEGVAHGYYFEEIGVE---ASSFG--------SWFTSW---DFNV----QIARVFTGGDFNFDLSRRKGLVVDAAHPSCDSPNISRRFALGDVRAWSGENPACTANNSTNYGESWATSAGEQYPWISAAEFAEDDAGGHGTHTAGSAAGAVVSSPSYSQTGDFTEVCEEGRELSCVGGCIDVDDPFGEDDLITTWDYQYFWAGYEAIDLDRLCPRL---DCDDLPEEYCLGDDEAETLQQHGGVARGAQLAIFDIINDYTGVGIDMAGNGMWEPAMEAGSKLSSNSWGIYAYCDYLSLDVLFDDFMYRNQDQLLLFAAGNDGD-DWSGCTMGTPALAKNVLAVGASTSGPGRLSFTDEDGDPTDGDSRVQDIDAMAYFSSFGPTLDNRIKPEVVAPGDMIYSAAGSGD-GNYTCRLWQYAGTSMSTPIVAGAAALVRQYFKNETFYAHDVEERGFCDSF-GCEPFAPSAATVKAMLINSANLMGGSAEPDGLRGFGRVHLEAGMPLAGEGDNALFVADSAVTSTAELTLTEYSFAVDAAAGIDLRATLSWIDPPATTFSSVQLVHDLDLSVYSPTGLRYSMWRDGTVDSLNNNERVIVPASNILDQTSSDATTAEDGESDGTWTVRVWSKRLAGNETQSYSLVVTGAIGPPT 1081          

BLAST of mRNA_Ecto-sp13_S_contig1258.2133.1 vs. uniprot
Match: A0A2I6U1Q9_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1Q9_SACJA)

HSP 1 Score: 624 bits (1610), Expect = 8.830e-204
Identity = 359/689 (52.10%), Postives = 425/689 (61.68%), Query Frame = 2

Query: 1010 GLDHLLPSGRGAGGRSDGPEAAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNAAARANIQSATVTNTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSFSAPTSDDDDSWGWPWATADHHTGSYYQIAEEFDGGDFTIYPDRRKIIQYINLIKSDDTEGSHGDYFTTSLGQQYPWLPADHFEYDYEAGHGTHVAGSVAGATLNTPAEL-ISCNDTETMSCVGACVDDDEANDVDDLATPYVEIA-DLDRLCPLFSDYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDSFGLSPFVGNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGELR--RSTCTMGSPAIAKNVLAVGASTSGDTRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYVGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG-CEAFSPSSVTLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLGGEGSLVLFVSDSNYTSIPERTRQEYNFDVNATAGLDLRVTLSWLDPPATTFSAVQLVHDLDLIVISPNGTRYTMWV-SGVTDSSNVNERVIVDAGDVETGTWSVWILADNLTTDVQSYSLVVNGAI 3058
            GLDHLL    GA G  +  EA EL +ACFMGLVSFL+S+ EVLRV+PR +  LLNA AR  IQSAT+T  PLTDAGLDGTGQV+QVI                                                                                      QYINLIK+D   G      TTS G +  W   DHF+ D   GHGTH AGS  GAT+ TPAE   +C+ TE   CVG C+D             Y +I  +LDR CP+     CD     +CLSDDV +TL N+GGMAQGAKLA FD F D   L+P+VGNGLWEPC EA CKIHS S GGD EC++   DVLYD +M +NPENL++ AAGN G+++  R+ CTM SPAI         S  GD          +DTVA FSSYGPT D RIKPEV+APGD  YSA SDG  D HSCRL A  GTSM+ PIVAGASAM+RQYFM+E+F+  D  ARG+CD+    CE+FSPSS T+KAL+INSANLMGGSSEPDG RGFGRIHLE GMPL G GSL LFV+DS+YTS+PE +  ++ FDV+A A LD R TLSW+DPPA++ S VQLVHDLDL V+SP+G  + MW  SG  DS NVNERVI+ A DVE+GT++V +  ++L TD Q YSLVVNGA+
Sbjct:   82 GLDHLLHKESGAIGSDE--EATELRVACFMGLVSFLSSRLEVLRVSPRHAGRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVI--------------------------------------------------------------------------------------QYINLIKADSVPGPVSS--TTSGGDKVRWFSPDHFQGDDIEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCID-----------AEYSQIGVNLDRFCPMIQ---CDDATDPRCLSDDVGQTLANHGGMAQGAKLAFFDIFADGGALTPYVGNGLWEPCLEAGCKIHSMSIGGDLECAVHGLDVLYDDFMYNNPENLVVVAAGNAGDIQDGRTVCTMYSPAIGTT------SADGDLADGTNGYADVDTVAQFSSYGPTQDGRIKPEVVAPGDTTYSARSDGG-DDHSCRLSAQFGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDDSGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGNGSLSLFVADSSYTSLPELSSHDFVFDVDADANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAADVESGTYTVRVSTNHLLTDSQGYSLVVNGAM 659