mRNA_Ecto-sp13_S_contig8689.20145.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8689.20145.1
Unique NamemRNA_Ecto-sp13_S_contig8689.20145.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A6H5KD36_9PHAE (eIF3g domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KD36_9PHAE)

HSP 1 Score: 271 bits (693), Expect = 1.070e-90
Identity = 139/150 (92.67%), Postives = 144/150 (96.00%), Query Frame = 1 
Query:   31 VNSVMPGRQAWGDDDXXDYLPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLESTNEVAPPEDEA 480
            VNS+MPGRQAWGD  XX YLPPRQESAVDS GVKSIVEYHFN+NGAKVKRTTKVKVFTEHTRVHKSCLERTQW KFGLAAQVDDNAS+++KSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLESTNE APPEDEA
Sbjct:   34 VNSIMPGRQAWGDXXXXXYLPPRQESAVDSNGVKSIVEYHFNANGAKVKRTTKVKVFTEHTRVHKSCLERTQWKKFGLAAQVDDNASHSMKSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLESTNEAAPPEDEA 183
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: D8LTT6_ECTSI (Putative initiation factor eIF3 g subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTT6_ECTSI)

HSP 1 Score: 194 bits (493), Expect = 1.880e-59
Identity = 103/145 (71.03%), Postives = 110/145 (75.86%), Query Frame = 1 
Query:   43 MPGRQAWGDDDXXDYLPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLESTNEVAPPEDE 477
            MPGRQAWGDDD  DYLPPRQESAVDSKGVKSIVEYHFN+NGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQ +DN S  IK  EE R E P++SR+ +E PNS  D LK         H MRT     S  E A PE+E
Sbjct:    1 MPGRQAWGDDDDDDYLPPRQESAVDSKGVKSIVEYHFNTNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQGEDNPSSPIKWREEFRREPPRDSRKPEENPNSRGDPLKGPRGPRGGNHPMRTGARRGSPKEAALPEEE 145
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A835YJN9_9STRA (Eukaryotic translation initiation factor 3 subunit G n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJN9_9STRA)

HSP 1 Score: 158 bits (400), Expect = 7.040e-45
Identity = 82/143 (57.34%), Postives = 106/143 (74.13%), Query Frame = 1 
Query:   52 RQAWGD-DDXXDYLPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLESTNEVAPPEDE 477
            R  WGD DD  DYLPPRQES VD KG+K++VE+ FN  G K K TTKVKVF E  R+ ++  ER +W  FG AA+V DN SYT+ S E++ METP+ES+EADE+P+++ D+LKEAMER+RR++AMR  G+L    E AP +DE
Sbjct:    2 RGNWGDEDDDPDYLPPRQESGVDGKGIKTVVEWAFNDAGQKTKTTTKVKVFAEQQRISRAVAERKKWKVFGAAAEVSDNHSYTLTSQEQVMMETPRESKEADEQPSNVSDSLKEAMERVRRRNAMRQMGML-GDGEAAPTDDE 143
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A0K9NXZ8_ZOSMR (Eukaryotic translation initiation factor 3 subunit G n=1 Tax=Zostera marina TaxID=29655 RepID=A0A0K9NXZ8_ZOSMR)

HSP 1 Score: 76.3 bits (186), Expect = 1.240e-13
Identity = 43/95 (45.26%), Postives = 54/95 (56.84%), Query Frame = 1 
Query:   61 WG---DDDXXDYLPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFT-EHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETP 333
            WG   D+D  D+LPP      D  GVK ++EY FN +GAKVK TT  +V      R+ K  +ER  W+KFG AA  D  A +T  S EEI +E P
Sbjct:   12 WGELEDEDDYDFLPPPLSIGPDENGVKKLIEYKFNDDGAKVKVTTTTRVRKLAKARLSKKAIERRAWSKFGDAANADPGARHTAVSTEEIILERP 106
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A6D2KV84_9BRAS (Eukaryotic translation initiation factor 3 subunit G n=1 Tax=Microthlaspi erraticum TaxID=1685480 RepID=A0A6D2KV84_9BRAS)

HSP 1 Score: 73.6 bits (179), Expect = 1.350e-12
Identity = 42/96 (43.75%), Postives = 58/96 (60.42%), Query Frame = 1 
Query:   61 WGD---DDXXDYL-PPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFT-EHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETP 333
            WG+   DD  D+L PP Q    D  G+K+++EY FN+ G KVK+TT+ ++      R+ K  +ER  W KFG AA  DD ++ T+ S EEI ME P
Sbjct:   14 WGEMEEDDNFDFLLPPMQVIGPDHTGLKTVIEYDFNAEGHKVKKTTRTRIKKLAIARLSKHAIERRNWPKFGDAANGDDGSNLTMMSTEEIIMERP 109
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A2U1P8Q5_ARTAN (Eukaryotic translation initiation factor 3 subunit G n=1 Tax=Artemisia annua TaxID=35608 RepID=A0A2U1P8Q5_ARTAN)

HSP 1 Score: 68.2 bits (165), Expect = 1.190e-10
Identity = 41/98 (41.84%), Postives = 54/98 (55.10%), Query Frame = 1 
Query:   61 WGD---DDXXDY---LPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFT-EHTRVHKSCLERTQWNKFGLAAQVDDNASYTIKSMEEIRMETP 333
            WG+   ++  DY   LPP+Q    D+ G+K  +EY FN  G KVK+TT  +V    + R+ K  LER  W+KFG A   D  A  T+ S EEI  E P
Sbjct:   11 WGELDEEEDTDYNYLLPPKQTIGPDNNGIKKTIEYSFNDEGHKVKKTTTTRVRKLANARLSKKALERRGWSKFGDAVHEDVGARLTMVSTEEIVFERP 108
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BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Match: A0A084G143_PSEDA (Eukaryotic translation initiation factor 3 subunit G n=2 Tax=Microascaceae TaxID=5593 RepID=A0A084G143_PSEDA)

HSP 1 Score: 55.1 bits (131), Expect = 5.790e-6
Identity = 30/75 (40.00%), Postives = 41/75 (54.67%), Query Frame = 1 
Query:   61 WGDDDXXDY----LPPRQESAVDSKGVKSIVEYHFNSNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQ 273
            W DD+  D     LPP Q +  +  G K+I+ YH N NG KVK T +V+  T   +V+    ER  W KFG +A+
Sbjct:    9 WADDEDIDEISTELPPAQ-TISNKDGTKTIITYHINENGQKVKTTRRVRYTTHTEKVNPRVAERKGWTKFGASAK 82
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8689.20145.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
A0A6H5KD36_9PHAE1.070e-9092.67eIF3g domain-containing protein n=1 Tax=Ectocarpus... [more]
D8LTT6_ECTSI1.880e-5971.03Putative initiation factor eIF3 g subunit n=1 Tax=... [more]
A0A835YJN9_9STRA7.040e-4557.34Eukaryotic translation initiation factor 3 subunit... [more]
A0A0K9NXZ8_ZOSMR1.240e-1345.26Eukaryotic translation initiation factor 3 subunit... [more]
A0A6D2KV84_9BRAS1.350e-1243.75Eukaryotic translation initiation factor 3 subunit... [more]
A0A2U1P8Q5_ARTAN1.190e-1041.84Eukaryotic translation initiation factor 3 subunit... [more]
A0A084G143_PSEDA5.790e-640.00Eukaryotic translation initiation factor 3 subunit... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8689contigEcto-sp13_S_contig8689:2037..4355 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score195.7
Seed ortholog evalue1.7e-47
Seed eggNOG ortholog2880.D8LTT6
Preferred nameTIF35
Model size481
KEGG koko:K03248,ko:K05666,ko:K20304
KEGG TC3.A.1.208.2
KEGG Pathwayko01523,ko01524,ko02010,ko03013,ko04976,map01523,map01524,map02010,map03013,map04976
Hectar predicted targeting categoryother localisation
GOsGO:0001732,GO:0002181,GO:0002183,GO:0002188,GO:0003674,GO:0003676,GO:0003723,GO:0003743,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005852,GO:0006412,GO:0006413,GO:0006415,GO:0006518,GO:0006807,GO:0008135,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009987,GO:0010467,GO:0010494,GO:0016043,GO:0016282,GO:0019538,GO:0022411,GO:0022607,GO:0022613,GO:0022618,GO:0032984,GO:0032991,GO:0034622,GO:0034641,GO:0034645,GO:0035770,GO:0036464,GO:0043043,GO:0043170,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043603,GO:0043604,GO:0043614,GO:0043624,GO:0043933,GO:0044085,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044424,GO:0044444,GO:0044464,GO:0065003,GO:0070993,GO:0071540,GO:0071541,GO:0071704,GO:0071826,GO:0071840,GO:0097159,GO:1901363,GO:1901564,GO:1901566,GO:1901576,GO:1990904
Exons4
EggNOG free text desc.translation initiation factor activity
EggNOG OGsKOG0122@1,KOG0122@2759
Ec32 ortholog descriptionputative initiation factor eIF3 g subunit
Ec32 orthologEc-28_002840.1
Cds size480
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko02000,ko03012,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464141.3644097-CDS-Ecto-sp13_S_contig8689:2036..20651681464141.3644097-CDS-Ecto-sp13_S_contig8689:2036..2065Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8689 2037..2065 +
1681464141.3866036-CDS-Ecto-sp13_S_contig8689:2927..30691681464141.3866036-CDS-Ecto-sp13_S_contig8689:2927..3069Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8689 2928..3069 +
1681464141.3978124-CDS-Ecto-sp13_S_contig8689:3583..36851681464141.3978124-CDS-Ecto-sp13_S_contig8689:3583..3685Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8689 3584..3685 +
1681464141.408244-CDS-Ecto-sp13_S_contig8689:4147..43541681464141.408244-CDS-Ecto-sp13_S_contig8689:4147..4354Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8689 4148..4354 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8689.20145.1prot_Ecto-sp13_S_contig8689.20145.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8689 2037..4354 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8689.20145.1

>prot_Ecto-sp13_S_contig8689.20145.1 ID=prot_Ecto-sp13_S_contig8689.20145.1|Name=mRNA_Ecto-sp13_S_contig8689.20145.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=160bp
GHTTFVRQFIVNSVMPGRQAWGDDDDDDYLPPRQESAVDSKGVKSIVEYH
FNSNGAKVKRTTKVKVFTEHTRVHKSCLERTQWNKFGLAAQVDDNASYTI
KSMEEIRMETPQESREADEKPNSMEDTLKEAMERMRRKHAMRTAGLLEST
NEVAPPEDEA
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mRNA from alignment at Ecto-sp13_S_contig8689:2037..4355+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8689.20145.1 ID=mRNA_Ecto-sp13_S_contig8689.20145.1|Name=mRNA_Ecto-sp13_S_contig8689.20145.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2319bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8689:2037..4355+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGTCATACTACCTTCGTTCGTCAGTTTATGTGAGTTATTTGCTTGTTTTG
CCGTGCGTCCTTTGTTTTGCCTGTGCGACGGCTCTGTATGGCTCTAACCC
TCATGGTGGATGGTTGTCGCGTTGTTAAGTCGTCGTGATTCTCGAGTGTG
CGTTAGGCCTTAGCGAGCCATGCAGGTTCTGGACCCATAGTGTTGATTCC
CACAACACGTCCTTCCTTGCAACGCAATGGTTACCATTCCCTCTTGGATG
TATGGTACGTGCTACGTGCACCATCTTTTTTCACACACCCATGCACGCCC
TCAATTCTGAACTGGAAGCAATGGACGTACGTGAAAACGCGTCTCGCCCG
CGACTACACTCCTTGGTAGCGAAAAACACCCCATGATTATCGATTGCACG
ACCATTCGAAGAAAAGGAAAGCGTGGGTGGACCATATTTTTCTGTTTGGT
GTTGTACTCTGTCGTCGTGTTTACGCACAGAGCATTCCAATAAAACGAGC
AATTTTTTGCACACCTGTCGACGTTGTAGCCAGCGTGCAGTACCCGTCGA
CGTAAACGAGCGGCAGCGGCCGCTCCAGTATTGCGCTCTGCATGTGCTGG
ACGTCGCAGCTTGTCTTCCACACTAACGCACTACTAAAATCGTTATTGCC
TGTTAGACCAAGTGCTGCTTTTCGTTTGGTCGAACGCGTCGCCATTAAAG
GTGCGCTGAGAGGTACACACGTCCTCTCACATCGGCCGAAAGTACGGACT
ACAAACACAACCACGGGGAATACAAGTGTGCTACCCTCCGACCTCCCACG
ATCGACCCTTTTCCCGCGTTCGCTCATGTGACAAAATCGATTCATTTTTT
GCGCTGCTGTCTTCTCTTTGCCACCTCTTTGTGGTCCCAAGTGTTAATTC
CGTCATGCCTGGACGACAAGCGTGGGGAGATGATGACGATGATGACTACT
TGCCCCCTCGCCAAGAGTCGGCGGTGGACTCTAAGGGCGTCAAGAGCATC
GTCGAGTACCACTTCAATTCCAACGGTGCTAAGGTGAGGGCGTGGCTGGC
TCGCTCGTTCGCTCGCTTCCGCGAAGCTTAGCACCAGAATACTGCCGCCC
GTAACTCTCGCTGCTTCTGCTTGTGAAACCTGTAATCGTGCTGCAGCTTG
GTCCACTCAGCTTCTTGCCGTGCCAAATTGAGTCGCATCAACACTTAACG
TAAGAACTGGCTTCGTGACAGGGCCCGCGACGAGGCTACCTGTCCATGGA
CCTCTATTACAGTAGACCGTGAGTCACAACTTGCATTTTTCTGACGGGAA
GATGGACAAAATGGAGCATATCACCAATGCCAGCCGCATTATGTCTTCGA
GACGCAAAACGTGGATTGCCCACGGTATGTAGCGTTCGTTCCCAATCGCG
CACAAGCTACAGGGGCACGATGTCAGCGAGCACTACCAAAATTGTTTTTG
GTGCCCCATTAAAAGTGATGCTGAAATAAAAAAAAACACTGCGACGTCAT
CAGTGATAATCAATCAATAACGATCAATGATGATTTAACAACAACAGGTG
AAGCGGACCACCAAGGTCAAGGTCTTCACGGAGCACACGCGAGTGCACAA
GTCTTGCCTGGAGCGTACGCAGTGGAACAAGTTCGGTCTCGCGGCGCAGG
TGTGCTACATGATGTTTTGATTCTCTTCTTTCCCCCCACGGGGAGAGGAC
TCACTGTTCCGCACCGGCTAGAAACTTTGGGATTATTTCACCCGCAACCT
GCATTCCCTCCCTTGCGCCGTGTCGCAAGAGATGCGTTGGCAAATGTGCA
CCGGTTGCCACAGGCTGACGCTATTAGAGTCGATTGACGGATGATTCCCG
GCCTAACAAGAATTCACGGTGTGTGGGGAGATCTTCCACCCCCCCTTGCT
CTCTGTAAAATCAATCGGAAGACGACGATGTCCTGGCAACTCCACCGTCC
CCGCGCTTCGTAGTACCCACCGAGCTCACTCCGACGCGATCGTTGTGTAT
TCCCACCCGCCCCATGTAGGTGGATCCGTCCTCTCGCGAGGACGCGCGGT
CAAATAGTCTAACCGCTAAAGCGTATTAAAAACATGCTCCCCCCACACCC
GCATGTAAAAGGTGGACGACAACGCCAGCTACACCATCAAGTCGATGGAG
GAGATCCGGATGGAGACGCCGCAGGAGTCGCGAGAGGCCGACGAGAAGCC
CAACAGCATGGAGGACACCTTGAAGGAGGCCATGGAACGTATGAGGAGGA
AGCATGCCATGAGGACGGCGGGACTGCTTGAATCCACCAATGAGGTCGCG
CCTCCGGAAGACGAAGCGG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8689:2037..4355+

>mRNA_Ecto-sp13_S_contig8689.20145.1 ID=mRNA_Ecto-sp13_S_contig8689.20145.1|Name=mRNA_Ecto-sp13_S_contig8689.20145.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=480bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8689:2037..4355+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGTCATACTACCTTCGTTCGTCAGTTTATTGTTAATTCCGTCATGCCTGG
ACGACAAGCGTGGGGAGATGATGACGATGATGACTACTTGCCCCCTCGCC
AAGAGTCGGCGGTGGACTCTAAGGGCGTCAAGAGCATCGTCGAGTACCAC
TTCAATTCCAACGGTGCTAAGGTGAAGCGGACCACCAAGGTCAAGGTCTT
CACGGAGCACACGCGAGTGCACAAGTCTTGCCTGGAGCGTACGCAGTGGA
ACAAGTTCGGTCTCGCGGCGCAGGTGGACGACAACGCCAGCTACACCATC
AAGTCGATGGAGGAGATCCGGATGGAGACGCCGCAGGAGTCGCGAGAGGC
CGACGAGAAGCCCAACAGCATGGAGGACACCTTGAAGGAGGCCATGGAAC
GTATGAGGAGGAAGCATGCCATGAGGACGGCGGGACTGCTTGAATCCACC
AATGAGGTCGCGCCTCCGGAAGACGAAGCG
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