mRNA_Ecto-sp13_S_contig8051.19384.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8051.19384.1
Unique NamemRNA_Ecto-sp13_S_contig8051.19384.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A6H5K1C0_9PHAE (Protein kinase domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5K1C0_9PHAE)

HSP 1 Score: 248 bits (634), Expect = 1.530e-80
Identity = 115/119 (96.64%), Postives = 118/119 (99.16%), Query Frame = 1 
Query:   22 TQVRASPHKNAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERFY 378
            + VRASPH+NAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKG+RHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERFY
Sbjct:  100 SDVRASPHRNAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGIRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERFY 218
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A482RBG5_9ARCH (Protein kinase domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RBG5_9ARCH)

HSP 1 Score: 162 bits (409), Expect = 3.330e-47
Identity = 74/120 (61.67%), Postives = 91/120 (75.83%), Query Frame = 1 
Query:   16 PHTQVRASPHKNAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERF 375
            P+ +   SP   A   AYL RQHL  +L +R  TRPFLT +EK WL+Y LLRA AQCH +G+ HGDIKSENV+VT+  W++LTDFA FKPTF+PDDHPAD +Y+F SG + RCY+APERF
Sbjct:  121 PYQRWEGSPKMGA---AYLFRQHLAHSLYERCHTRPFLTRSEKMWLIYQLLRALAQCHARGIAHGDIKSENVMVTAWGWLMLTDFALFKPTFIPDDHPADFHYFFESGTRRRCYIAPERF 237
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A7S1CBY9_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CBY9_9STRA)

HSP 1 Score: 152 bits (385), Expect = 5.780e-44
Identity = 70/105 (66.67%), Postives = 87/105 (82.86%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGE-QGRCYLAPERF 375
            A+L+RQH+  +L +R+STRPFL+D EKRW+ + LLRA AQCHG GV HGD+KSENVLVTS NWVLLTDFAPFKPT LP+D  +D +Y+F +G  + RCY+APERF
Sbjct:  116 AFLVRQHVQWSLYERISTRPFLSDVEKRWITFQLLRALAQCHGAGVVHGDVKSENVLVTSWNWVLLTDFAPFKPTRLPEDDHSDFHYFFDAGSGRRRCYVAPERF 220
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A7S2ST98_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2ST98_9STRA)

HSP 1 Score: 155 bits (392), Expect = 3.410e-43
Identity = 71/106 (66.98%), Postives = 83/106 (78.30%), Query Frame = 1 
Query:   67 YLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQG--RCYLAPERFY 378
            YLLRQH   +L DRLSTRPFL+  EK+W+VY L +A  QCH  GV HGDIKSENV+VTS  W++LTDFA FKPT+LP+D P D N+YF+S E G  RCYLAPERFY
Sbjct:  114 YLLRQHFYTSLYDRLSTRPFLSTVEKKWIVYQLFKALIQCHDSGVCHGDIKSENVMVTSWTWIILTDFASFKPTYLPEDDPTDFNHYFASAEHGQHRCYLAPERFY 219
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A6I9NUT4_9TELE (phosphoinositide 3-kinase regulatory subunit 4 n=1 Tax=Notothenia coriiceps TaxID=8208 RepID=A0A6I9NUT4_9TELE)

HSP 1 Score: 147 bits (370), Expect = 2.520e-41
Identity = 66/104 (63.46%), Postives = 80/104 (76.92%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERF 375
            A L RQ++   L DR+STRPFL + EKRWL + +L A  Q H  GVRHGDIK+ENV+VTS NWVLLTDFA FKPTFLP+D+PAD NY+F +  +  CY+APERF
Sbjct:   97 AILFRQYVRDNLYDRISTRPFLNNVEKRWLAFQILNAVDQAHKAGVRHGDIKTENVMVTSWNWVLLTDFASFKPTFLPEDNPADFNYFFDTSRRRTCYIAPERF 200
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A3B3Z8Y1_9GOBI (Protein kinase domain-containing protein n=2 Tax=Oxudercinae TaxID=497678 RepID=A0A3B3Z8Y1_9GOBI)

HSP 1 Score: 146 bits (369), Expect = 3.570e-41
Identity = 69/115 (60.00%), Postives = 85/115 (73.91%), Query Frame = 1 
Query:   31 RASPHKNAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERF 375
            +AS  + AG    L RQ++   L DR+STRPFL + EKRWL + +L A  Q H  GVRHGDIKSEN++VTS NWVLLTDFA FKPTFLP+D+PAD NY+F +  +  CY+APERF
Sbjct:   89 KASLTEKAGI---LFRQYVRDNLYDRISTRPFLNNIEKRWLAFQILSAVEQSHRSGVRHGDIKSENIMVTSWNWVLLTDFASFKPTFLPEDNPADFNYFFDTSRRRTCYIAPERF 200
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A699HXK4_TANCI (Armadillo-like helical (Fragment) n=1 Tax=Tanacetum cinerariifolium TaxID=118510 RepID=A0A699HXK4_TANCI)

HSP 1 Score: 145 bits (366), Expect = 3.810e-41
Identity = 65/105 (61.90%), Postives = 79/105 (75.24%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERFY 378
            AYLLRQ+    L DRLSTRPFL+  EK+WL + LL A  QCH  G+ HGDIK ENVLVTS NW+ L DFA FKPT++P D P+D +++F +G + RCYLAPERFY
Sbjct:   72 AYLLRQYFFNNLHDRLSTRPFLSLVEKKWLAFQLLYAVKQCHDNGICHGDIKCENVLVTSWNWLYLADFASFKPTYIPHDDPSDFSFFFDTGGRRRCYLAPERFY 176
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A672L4V0_SINGR (Phosphoinositide 3-kinase regulatory subunit 4-like n=3 Tax=Clupeocephala TaxID=186625 RepID=A0A672L4V0_SINGR)

HSP 1 Score: 145 bits (365), Expect = 1.780e-40
Identity = 64/104 (61.54%), Postives = 80/104 (76.92%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERF 375
            A L RQ++   L DR+STRPFL + EK+W+ + LL A  Q H  GVRHGDIK+ENV+VTS NWVLLTDFA FKPT+LP+D+PAD NY+F +  +  CY+APERF
Sbjct:   97 AILFRQYVRDNLYDRISTRPFLNNVEKKWIAFQLLNAVDQAHKSGVRHGDIKTENVMVTSWNWVLLTDFASFKPTYLPEDNPADFNYFFDTSRRRTCYIAPERF 200
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: UPI001ABC4D1D (serine/threonine-protein kinase VPS15-like n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=UPI001ABC4D1D)

HSP 1 Score: 142 bits (358), Expect = 2.460e-40
Identity = 64/102 (62.75%), Postives = 78/102 (76.47%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPE 369
            AYLLRQ+    L DRLSTRPFL+  EK+WL + L+ A  Q H KGV HGDIK ENVLVTS NW+ LTDFA FKPT++PDD P+D +++F +G + RCYLAPE
Sbjct:  101 AYLLRQYSYSNLHDRLSTRPFLSQIEKKWLAFQLIHAVEQSHSKGVCHGDIKCENVLVTSWNWLYLTDFASFKPTYIPDDDPSDFSFFFDTGGRRRCYLAPE 202
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BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Match: A0A6P8THR0_GYMAC (phosphoinositide 3-kinase regulatory subunit 4-like n=1 Tax=Gymnodraco acuticeps TaxID=8218 RepID=A0A6P8THR0_GYMAC)

HSP 1 Score: 147 bits (370), Expect = 2.510e-40
Identity = 66/104 (63.46%), Postives = 80/104 (76.92%), Query Frame = 1 
Query:   64 AYLLRQHLLGTLSDRLSTRPFLTDTEKRWLVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPDDHPADANYYFSSGEQGRCYLAPERF 375
            A L RQ++   L DR+STRPFL + EKRWL + +L A  Q H  GVRHGDIK+ENV+VTS NWVLLTDFA FKPTFLP+D+PAD NY+F +  +  CY+APERF
Sbjct:   97 AILFRQYVRDNLYDRISTRPFLNNVEKRWLAFQILNAVDQAHKAGVRHGDIKTENVMVTSWNWVLLTDFASFKPTFLPEDNPADFNYFFDTSRRRTCYIAPERF 200
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8051.19384.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5K1C0_9PHAE1.530e-8096.64Protein kinase domain-containing protein n=1 Tax=E... [more]
A0A482RBG5_9ARCH3.330e-4761.67Protein kinase domain-containing protein (Fragment... [more]
A0A7S1CBY9_9STRA5.780e-4466.67Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A7S2ST98_9STRA3.410e-4366.98Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A6I9NUT4_9TELE2.520e-4163.46phosphoinositide 3-kinase regulatory subunit 4 n=1... [more]
A0A3B3Z8Y1_9GOBI3.570e-4160.00Protein kinase domain-containing protein n=2 Tax=O... [more]
A0A699HXK4_TANCI3.810e-4161.90Armadillo-like helical (Fragment) n=1 Tax=Tanacetu... [more]
A0A672L4V0_SINGR1.780e-4061.54Phosphoinositide 3-kinase regulatory subunit 4-lik... [more]
UPI001ABC4D1D2.460e-4062.75serine/threonine-protein kinase VPS15-like n=1 Tax... [more]
A0A6P8THR0_GYMAC2.510e-4063.46phosphoinositide 3-kinase regulatory subunit 4-lik... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8051contigEcto-sp13_S_contig8051:1386..2359 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeFungi
Stop0
Start0
Seed ortholog score147.9
Seed ortholog evalue3.2e-33
Seed eggNOG ortholog13349.G1XIM0
Preferred nameVPS15
Model size378
KEGG koko:K08333
KEGG Pathwayko04136,ko04138,ko04140,ko04371,map04136,map04138,map04140,map04371
Hectar predicted targeting categoryother localisation
GOsGO:0000011,GO:0000166,GO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005488,GO:0005515,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005643,GO:0005737,GO:0005739,GO:0005768,GO:0005770,GO:0005773,GO:0005774,GO:0005942,GO:0006355,GO:0006357,GO:0006464,GO:0006468,GO:0006605,GO:0006623,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006886,GO:0006914,GO:0006996,GO:0007033,GO:0007034,GO:0007154,GO:0007165,GO:0008104,GO:0008144,GO:0008150,GO:0008152,GO:0009056,GO:0009889,GO:0009891,GO:0009893,GO:0009987,GO:0010468,GO:0010556,GO:0010557,GO:0010604,GO:0010628,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016043,GO:0016192,GO:0016197,GO:0016236,GO:0016301,GO:0016310,GO:0016482,GO:0016740,GO:0016772,GO:0016773,GO:0017076,GO:0019219,GO:0019222,GO:0019538,GO:0019898,GO:0023052,GO:0030242,GO:0030554,GO:0031090,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0031410,GO:0031967,GO:0031975,GO:0031982,GO:0032182,GO:0032507,GO:0032553,GO:0032555,GO:0032559,GO:0032784,GO:0032786,GO:0032968,GO:0032991,GO:0033036,GO:0033365,GO:0034067,GO:0034243,GO:0034271,GO:0034272,GO:0034613,GO:0035032,GO:0035556,GO:0035639,GO:0036094,GO:0036211,GO:0042147,GO:0042886,GO:0043130,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044232,GO:0044237,GO:0044238,GO:0044248,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0045053,GO:0045184,GO:0045185,GO:0045324,GO:0045893,GO:0045935,GO:0045944,GO:0046907,GO:0048017,GO:0048308,GO:0048518,GO:0048522,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051173,GO:0051179,GO:0051234,GO:0051235,GO:0051252,GO:0051254,GO:0051641,GO:0051649,GO:0051651,GO:0051716,GO:0060255,GO:0061695,GO:0061919,GO:0065007,GO:0065008,GO:0070727,GO:0071561,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0072594,GO:0072665,GO:0072666,GO:0080090,GO:0097159,GO:0097367,GO:0097708,GO:0098588,GO:0098796,GO:0098805,GO:0098852,GO:0140096,GO:1901265,GO:1901363,GO:1901564,GO:1902494,GO:1902680,GO:1903506,GO:1903508,GO:1990234,GO:2000112,GO:2001141
Exons2
EggNOG free text desc.VPS15 protein kinase
EggNOG OGs38C2I@33154,3NXFW@4751,3QPNA@4890,KOG1240@1,KOG1240@2759
Ec32 ortholog descriptionSerine/threonine/dual specificity protein kinase, catalytic domain
Ec32 orthologEc-08_002020.1
EC2.7.11.1
Cds size378
COG Functional cat.T
Best tax levelAscomycota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01001,ko04131
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464078.365414-CDS-Ecto-sp13_S_contig8051:1385..14661681464078.365414-CDS-Ecto-sp13_S_contig8051:1385..1466Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8051 1386..1466 -
1681464078.3836865-CDS-Ecto-sp13_S_contig8051:2062..23591681464078.3836865-CDS-Ecto-sp13_S_contig8051:2062..2359Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8051 2063..2359 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8051.19384.1prot_Ecto-sp13_S_contig8051.19384.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8051 1386..2359 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8051.19384.1

>prot_Ecto-sp13_S_contig8051.19384.1 ID=prot_Ecto-sp13_S_contig8051.19384.1|Name=mRNA_Ecto-sp13_S_contig8051.19384.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=126bp
TENGSPHTQVRASPHKNAGTPAYLLRQHLLGTLSDRLSTRPFLTDTEKRW
LVYLLLRAAAQCHGKGVRHGDIKSENVLVTSGNWVLLTDFAPFKPTFLPD
DHPADANYYFSSGEQGRCYLAPERFY
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mRNA from alignment at Ecto-sp13_S_contig8051:1386..2359-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8051.19384.1 ID=mRNA_Ecto-sp13_S_contig8051.19384.1|Name=mRNA_Ecto-sp13_S_contig8051.19384.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=974bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8051:1386..2359- (Ectocarpus species13 EcNAP12_S_4_19m)
ACCGAAAACGGCTCTCCACACACGCAGGTCCGCGCGAGCCCGCACAAGAA
CGCGGGCACCCCGGCGTACCTGCTACGGCAGCACCTCCTGGGGACTCTGA
GCGATCGGCTTTCCACCCGGCCTTTCCTCACCGACACCGAGAAGCGCTGG
CTCGTGTACCTCCTGCTGAGGGCCGCGGCGCAGTGCCACGGGAAGGGGGT
ACGTCACGGCGACATCAAGAGCGAGAACGTGCTGGTGACGAGCGGGAACT
GGGTGCTGCTGACGGACTTCGCTCCGTTCAAGCCCACCTTCTTGCCAGTG
AGGAGGAGGAGGAGGAGGAGGAGGGTGTCGGGACGGGTCTGTCGTCTCTC
GCCTCGGTGCCGGCAACACCATCGTTGCTCGAGTACCGTATAGTCCCTGT
GCCACAAGTGTTGCTTGCTGAGAGTGTGTGCAGCAGCATGGTCGTGCTGA
AAGGGCCGGTGTGGTTGCTCTTCCTTGAAAGTTTTAAAGCGCACGATTTG
GGTGAGGTGCCACCTGGTATGATACACCCCGTGCCCTTGTTTCAGCGGTG
TTGTTACTTCTACAGGCTGGGGCGTTGTACGCGCGACGTGTTGACTCTGC
CTGCAACGGCGAAGTGGGATTCGTGCGTACTCTGTCTCGAACGCGATGCC
TGACCAGGCACAGCAGCACAACAATCTCCAGGCTTACACACCAAGTATAT
ATGCACCCCACACACAAAACACATCCGCGCCTGGCAAAGTAGGAAGGAAC
GATTGGCCATCGGTGATCGGCCCTGTGGGAACAATGCTCCGAGGACGCGT
GGCAAATAAATGGTCGAACCGCTATGGCATACCAAACATAAGAGTACGTC
CCCGGCCCCCCCCCCACGCGTCCCCCGCCCGCCCCCTACTCAGGATGACC
ACCCGGCGGACGCGAACTACTACTTCAGCTCGGGAGAGCAGGGCCGCTGC
TACCTTGCTCCCGAACGCTTTTAC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8051:1386..2359-

>mRNA_Ecto-sp13_S_contig8051.19384.1 ID=mRNA_Ecto-sp13_S_contig8051.19384.1|Name=mRNA_Ecto-sp13_S_contig8051.19384.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=378bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8051:1386..2359- (Ectocarpus species13 EcNAP12_S_4_19m)
ACCGAAAACGGCTCTCCACACACGCAGGTCCGCGCGAGCCCGCACAAGAA
CGCGGGCACCCCGGCGTACCTGCTACGGCAGCACCTCCTGGGGACTCTGA
GCGATCGGCTTTCCACCCGGCCTTTCCTCACCGACACCGAGAAGCGCTGG
CTCGTGTACCTCCTGCTGAGGGCCGCGGCGCAGTGCCACGGGAAGGGGGT
ACGTCACGGCGACATCAAGAGCGAGAACGTGCTGGTGACGAGCGGGAACT
GGGTGCTGCTGACGGACTTCGCTCCGTTCAAGCCCACCTTCTTGCCAGAT
GACCACCCGGCGGACGCGAACTACTACTTCAGCTCGGGAGAGCAGGGCCG
CTGCTACCTTGCTCCCGAACGCTTTTAC
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