mRNA_Ecto-sp13_S_contig6174.16872.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig6174.16872.1
Unique NamemRNA_Ecto-sp13_S_contig6174.16872.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: D8LG86_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LG86_ECTSI)

HSP 1 Score: 270 bits (691), Expect = 3.150e-88
Identity = 203/235 (86.38%), Postives = 207/235 (88.09%), Query Frame = 1 
Query:    1 QGMGPKRPSTGSMSARATANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQLALERENNARTQGRNSSNHERALAQRKQKQQHDLQSTKANLEKQRXXXXXXXXXXXXXLQMTHXXXXXXXXXXXXXXTEMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIEAEKKKTQEAERMIGKMEQDEDVLIERLKKVQDEQRKAYELLQESLES 705
            +GMGPKRPSTGSMSARATANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQLALERE+NARTQG+NSSNHERALAQRKQKQQHDLQSTKANLEKQR    XXXXXXXX LQMT+ XXXXXXX      TEMV XXXX          XXXXXXXXXXXXXRIEAE   TQEAERMI KMEQDEDVLIERLKKVQDEQRKAYELL ESLES
Sbjct:   27 KGMGPKRPSTGSMSARATANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQLALERESNARTQGKNSSNHERALAQRKQKQQHDLQSTKANLEKQRRTNAXXXXXXXXALQMTYQXXXXXXXAENRRRTEMVRXXXXEARLKKEAEQXXXXXXXXXXXXXRIEAEXXXTQEAERMIEKMEQDEDVLIERLKKVQDEQRKAYELLHESLES 261
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A6V1KT72_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1KT72_HETAK)

HSP 1 Score: 72.0 bits (175), Expect = 2.440e-11
Identity = 95/216 (43.98%), Postives = 128/216 (59.26%), Query Frame = 1 
Query:   55 ANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQLALERENNARTQGRNSSNHERALAQRKQKQQHDLQSTKANLEKQRXXXXXXXXXXXXXLQMTHXXXXXXXXXXXXXXTEMVXXXXXXXXXXXXXXXXXXXXXXXXXXXXRIEAEKKKTQEAERMIGKMEQDEDVLIERLKKVQDEQRKAYELLQESLE 702
            A+ ML+AKN+RKRAEADV LL NRL HLQ EEE+A +K  ETK RA EI +LK++N    + +++   +     +  ++ +   + +Q+  L  TK  L++ R             L             XXXXX    XXXXXXXXXXXXXXXXXXXXX       +I+AE  +  EAER+I ++EQ E  +I RLKK Q++QR AYE L+ SL+
Sbjct:   56 ASAMLDAKNSRKRAEADVKLLQNRLKHLQMEEERAFKKVYETKSRAKEILKLKKRNTDHMMNKQSYNHSMSALLNETQQNITSTRLQQKDRLNMTKQTLQEDRTWQGNQGKAQKEQLNAEARRQREGEYRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXLQEEYARKIKAEADRRTEAERLIEELEQQEARMINRLKKAQEDQRSAYEDLRTSLD 271
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A812UQ84_SYMMI (Hypothetical protein (Fragment) n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812UQ84_SYMMI)

HSP 1 Score: 60.1 bits (144), Expect = 2.370e-7
Identity = 36/59 (61.02%), Postives = 47/59 (79.66%), Query Frame = 1 
Query:   55 ANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQL 231
            A+ MLE KN+RKRAE D+ LLANR+A L+AEEE+A+ K  ETKQRA EI  LK++N+ +
Sbjct:   12 ASAMLELKNSRKRAEGDMQLLANRVALLKAEEERAKSKVRETKQRAKEILDLKKRNEDM 70
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A421GE68_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A421GE68_9STRA)

HSP 1 Score: 57.4 bits (137), Expect = 4.560e-6
Identity = 32/56 (57.14%), Postives = 46/56 (82.14%), Query Frame = 1 
Query:   58 NLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQ 225
            N ML+AKN  K+AEAD  LLANRLAHL+AE+ +A+R+ EE K+R+ EI+ +K++N+
Sbjct:  119 NAMLDAKNQSKQAEADAQLLANRLAHLRAEDARAQRRIEEAKRRSREIEAIKKRNE 174
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A6A3X7B1_9STRA (Uncharacterized protein n=3 Tax=Phytophthora TaxID=4783 RepID=A0A6A3X7B1_9STRA)

HSP 1 Score: 55.1 bits (131), Expect = 2.750e-5
Identity = 30/54 (55.56%), Postives = 45/54 (83.33%), Query Frame = 1 
Query:   64 MLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQ 225
            ML+AKN R++AE D  LLANRLAHL+AE+ +A+++ EE K+RA EI+ +K++N+
Sbjct:  122 MLDAKNQRRQAEVDAQLLANRLAHLRAEDARAQKRIEEAKRRAREIESIKKRNE 175
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A024TRB7_9STRA (EF-hand domain-containing protein n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TRB7_9STRA)

HSP 1 Score: 55.1 bits (131), Expect = 3.010e-5
Identity = 35/66 (53.03%), Postives = 48/66 (72.73%), Query Frame = 1 
Query:   25 STGSMSARATANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQN 222
            ST    A +  + MLEAK  RKRAE DV LLANRLAHL+ EE+KA++K +E  +RA +I+ +K++N
Sbjct:  147 STFMADATSIQSAMLEAKMQRKRAEEDVQLLANRLAHLRTEEKKAQKKIDEANKRAEDIEAIKRRN 212
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A662WV05_9STRA (Pterin-binding domain-containing protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WV05_9STRA)

HSP 1 Score: 54.7 bits (130), Expect = 5.270e-5
Identity = 30/54 (55.56%), Postives = 46/54 (85.19%), Query Frame = 1 
Query:   64 MLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQ 225
            ML+AKN RK+AEAD  LLANRLAHL+AE+ +A+++ EE ++RA EI+ ++++N+
Sbjct:  522 MLDAKNQRKQAEADAQLLANRLAHLRAEDARAQKRIEEAQRRAKEIEAVRKRNE 575
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A225VTZ1_9STRA (Uncharacterized protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225VTZ1_9STRA)

HSP 1 Score: 53.9 bits (128), Expect = 6.710e-5
Identity = 30/54 (55.56%), Postives = 44/54 (81.48%), Query Frame = 1 
Query:   64 MLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQ 225
            ML+AK  RK+AE D  LLANRLAHL+AE+ +A+++ EE K+RA EI+ +K++N+
Sbjct:  120 MLDAKKQRKQAEVDAQLLANRLAHLRAEDARAQKRIEEAKRRAREIEAIKKRNE 173
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BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Match: A0A2P4WY89_9STRA (Dihydropteroate synthase n=1 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4WY89_9STRA)

HSP 1 Score: 53.9 bits (128), Expect = 8.410e-5
Identity = 30/57 (52.63%), Postives = 45/57 (78.95%), Query Frame = 1 
Query:   58 NLMLEAKNTRKRAEADVHLLANRLAHLQAEEEKARRKTEETKQRAMEIKRLKQQNQQ 228
            N  L+AK  RK+AE D  LLANRLAHL+AE+ +A+++ EE K+RA EI+ +K++N++
Sbjct:  280 NATLDAKKQRKQAEVDAQLLANRLAHLRAEDARAQKRIEEAKRRAREIEAIKKRNEE 336
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig6174.16872.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 9
Match NameE-valueIdentityDescription
D8LG86_ECTSI3.150e-8886.38Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6V1KT72_HETAK2.440e-1143.98Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A812UQ84_SYMMI2.370e-761.02Hypothetical protein (Fragment) n=1 Tax=Symbiodini... [more]
A0A421GE68_9STRA4.560e-657.14Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
A0A6A3X7B1_9STRA2.750e-555.56Uncharacterized protein n=3 Tax=Phytophthora TaxID... [more]
A0A024TRB7_9STRA3.010e-553.03EF-hand domain-containing protein n=2 Tax=Aphanomy... [more]
A0A662WV05_9STRA5.270e-555.56Pterin-binding domain-containing protein (Fragment... [more]
A0A225VTZ1_9STRA6.710e-555.56Uncharacterized protein n=1 Tax=Phytophthora megak... [more]
A0A2P4WY89_9STRA8.410e-552.63Dihydropteroate synthase n=1 Tax=Phytophthora palm... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig6174contigEcto-sp13_S_contig6174:5517..8166 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score275.4
Seed ortholog evalue2.5e-71
Seed eggNOG ortholog2880.D8LG86
Model size716
Hectar predicted targeting categoryother localisation
Exons5
EggNOG OGs2AE5J@1,2RYV4@2759
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-17_004140.1
Cds size702
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig6174.16872.1prot_Ecto-sp13_S_contig6174.16872.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig6174 5525..8160 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463859.4681132-UTR-Ecto-sp13_S_contig6174:5516..55241681463859.4681132-UTR-Ecto-sp13_S_contig6174:5516..5524Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig6174 5517..5524 -
1681463859.5316856-UTR-Ecto-sp13_S_contig6174:8160..81661681463859.5316856-UTR-Ecto-sp13_S_contig6174:8160..8166Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig6174 8161..8166 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463859.4798586-CDS-Ecto-sp13_S_contig6174:5524..56531681463859.4798586-CDS-Ecto-sp13_S_contig6174:5524..5653Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6174 5525..5653 -
1681463859.4898317-CDS-Ecto-sp13_S_contig6174:6160..63191681463859.4898317-CDS-Ecto-sp13_S_contig6174:6160..6319Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6174 6161..6319 -
1681463859.5022078-CDS-Ecto-sp13_S_contig6174:6831..69181681463859.5022078-CDS-Ecto-sp13_S_contig6174:6831..6918Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6174 6832..6918 -
1681463859.5121934-CDS-Ecto-sp13_S_contig6174:7177..73241681463859.5121934-CDS-Ecto-sp13_S_contig6174:7177..7324Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6174 7178..7324 -
1681463859.5216422-CDS-Ecto-sp13_S_contig6174:7980..81601681463859.5216422-CDS-Ecto-sp13_S_contig6174:7980..8160Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig6174 7981..8160 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig6174.16872.1

>prot_Ecto-sp13_S_contig6174.16872.1 ID=prot_Ecto-sp13_S_contig6174.16872.1|Name=mRNA_Ecto-sp13_S_contig6174.16872.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=234bp
MGPKRPSTGSMSARATANLMLEAKNTRKRAEADVHLLANRLAHLQAEEEK
ARRKTEETKQRAMEIKRLKQQNQQLALERENNARTQGRNSSNHERALAQR
KQKQQHDLQSTKANLEKQRRTNAKSAREQRQALQMTHQELRAQKAAENRR
RTEMVRKQREEARLKKEAEQREREAKQQEEHRRRIEAEKKKTQEAERMIG
KMEQDEDVLIERLKKVQDEQRKAYELLQESLES*
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mRNA from alignment at Ecto-sp13_S_contig6174:5517..8166-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig6174.16872.1 ID=mRNA_Ecto-sp13_S_contig6174.16872.1|Name=mRNA_Ecto-sp13_S_contig6174.16872.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2650bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6174:5517..8166- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGGGCATGGGGCCAAAGAGGCCGTCAACAGGCTCGATGTCTGCTCGCGC
TACGGCCAACCTCATGCTGGAAGCCAAGAACACGAGAAAGAGAGCAGAAG
CTGACGTCCACCTCCTAGCAAATCGGTTAGCTCATCTACAAGCGGAGGAA
GAGAAAGCCAGACGAAAAACGGAAGAGACGAAGCAGGTTTACAAACATGC
AGTTTGCCCACCCTTGGAAGGCCTGTGATTTGGTGAAGACGTGCGACTGC
TTTTCAGAGAGCCATATGACATTTAGAGCTCATTATTCTGTGTAGCGATG
TCTCTACTGTCATTGTTGTCAAGGAGTAATCGGGCACAAAGCTCTGCATA
AAACTGTCACCCAGGCCTCTCTTCCGCGTAAGGCGTCCTCGAAAACGGAC
GATTAAGTTCAGTTGTCCGAACCAAGCAAAAATTAGCAAATGCCATGCAA
GGTACACATGAACAGCGAGCTTCGGAAAAAACATTGAAACTCGCGAAACC
AGTAAATAAAGTCAGCTGTTTTCGGCGGAGCCACTCAGCCCCAAACAATG
AGCAGCAAAGAGGCAGAAGAGCTTTATTTAGCTTGGAGTCAAAAAAGTGC
GTCACTCCAACTCGGAAGAGAAGTGGCACAACCAGAATTGGCTTCCCCTC
TGCACCTACTGTACATTCATCCGCAGGTCACATGCCATGGGTTCTCCCTT
CAAGTTGGTCAGTTTTAAGTGATGCCCCCGGTGTCCTTCTTAACCAATGA
CAATCCTCCTTGCCAATCCTAGCCCAAAGGTACCGCCCTAAGGTGTTGCC
TCTTGTGTGTTGCGACGCGCGGCTCGCCACTTGTTCTCGCAGCGCGCAAT
GGAGATCAAGAGACTGAAACAGCAGAACCAGCAACTTGCGCTTGAACGGG
AGAACAACGCGCGGACGCAGGGCAGGAATAGCAGCAACCACGAGCGAGCT
CTGGCGCAAAGAAAGCAGAAGCAACAACACGACTTGCAGGTGCAGTCCCG
TTGGAGAGAGAAAGAGTGCTCGAATGTCAGGAATACGATCCCCGGGAAGC
TGCCCGAGACACATCTTACGTATTTCCATGTACCACAACGATGTCAAAAT
GTGACGCATTAGATGAGGGATTTGACCCAACAGGCATTTTACGGGGTCTC
ATGACACGTAGTTCATTGGAAAGTCTCTCTTAACAATAAACTATTTTGAA
TTGCCGGTCCTCCTCCTGGACCCCCCCCCCACACGATGGCGTCTGCAGTC
CACCAAGGCCAACCTGGAGAAGCAAAGGAGGACCAACGCGAAGTCTGCTA
GAGAACAGCGACAGGCGTTGCAAATGACCCACCAGGTGCCTATCTCGCAT
GCCGTTTCGTTGCTGAGGCTGAAGTATCTGCTTCTTCTTCAAAGACGTCA
GCACCATCAGCCTTCGGAAGACACTCGTTGCGAAATAAAACGAATTTCCC
ATATGGAAGCAACACTTCCAATACTTACCAACCTTGTCAAAAGGGACTGC
GTTGCGTCAAAGTAAATCTTGGGCGTGATCAATCAAAGCACCTTTGTCAA
AGGCACGTACAAAGTCGAAGAGACAATCGACAGAAAAACCACCGTTTTCC
TTTCCAGCGGAAATCTCCGGGAAGGGTAGCCGAAACTGGACATCAGCCAT
ATCGAAGCTTTAGCAGTTAGACGTCTCCAGGTCGAATCAGATACGCGATC
AATGATGGCATTCGCCACTCGAAAGAACGCGGGGTAACCATGTACTCTGG
GACATCAGCCATCAGAGTATGACAACAGTGCCCCAAGCCTGTCGGTTGGT
TTTTCATCGAACACTATCCCGGGTTGGATTCACTTGCTGCTTGACAGGAG
CTGCGAGCACAGAAGGCCGCGGAGAATAGACGGAGGACCGAGATGGTCCG
GAAGCAGCGCGAGGAAGCTCGCCTAAAGAAGGAGGCCGAACAGAGGGAGA
GGGAAGCGAAGCAGCAAGAAGAGCACCGCCGGCGAATCGAAGCCGAGAAA
AAGAAGGTACTCCTGCACACCACGGGCGGGAGCATGATCTGGCATAGCCT
TGACGGCGATGCGGGGAATGGGGGTAGATGGTGGCGATTGTTGTTCTGTT
GTGTTTTGGCACAATGTGGATTGACTTGATCAGGGTGGTTGTTTCCTCCT
AGGGCGAGCAGCATACAGACCGCGTTCATGTAGACTTACCCCGGCGAAGT
CGTAGCAACGCAGCTGTGCTTTGCGCTCGGAAGATGCCTTGTCTCTTTTG
AGTTGCGCTGGGATCTGATCAGAAGAGTTGAGACAAGAACCGATCCCCAC
AATCCATCCGCATTGCGATCCACCTTCCTCACAAGCAGGCGGTTCCCTTC
TTGCAAGGCTTACTTCTGATATTTCCTTTGGGGCGTACATATGCTGAACA
AAAAATCTTAGGGTTAATGACACCCAAATGTTGCCCAATGCCACCGCGGC
CAGATCCTATGCAACAGTGTACGGCCTAAACAAGCCCCCCCCGCCGGCCA
CGCGTTTTGGCAGACACAAGAAGCAGAGCGCATGATTGGAAAGATGGAGC
AGGATGAGGACGTTTTGATCGAACGCCTCAAGAAGGTCCAAGACGAGCAG
CGTAAAGCGTACGAGTTGCTTCAGGAATCTCTTGAAAGCTGACATTTGGC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig6174:5517..8166-

>mRNA_Ecto-sp13_S_contig6174.16872.1 ID=mRNA_Ecto-sp13_S_contig6174.16872.1|Name=mRNA_Ecto-sp13_S_contig6174.16872.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=702bp|location=Sequence derived from alignment at Ecto-sp13_S_contig6174:5517..8166- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGGGCCAAAGAGGCCGTCAACAGGCTCGATGTCTGCTCGCGCTACGGC
CAACCTCATGCTGGAAGCCAAGAACACGAGAAAGAGAGCAGAAGCTGACG
TCCACCTCCTAGCAAATCGGTTAGCTCATCTACAAGCGGAGGAAGAGAAA
GCCAGACGAAAAACGGAAGAGACGAAGCAGCGCGCAATGGAGATCAAGAG
ACTGAAACAGCAGAACCAGCAACTTGCGCTTGAACGGGAGAACAACGCGC
GGACGCAGGGCAGGAATAGCAGCAACCACGAGCGAGCTCTGGCGCAAAGA
AAGCAGAAGCAACAACACGACTTGCAGTCCACCAAGGCCAACCTGGAGAA
GCAAAGGAGGACCAACGCGAAGTCTGCTAGAGAACAGCGACAGGCGTTGC
AAATGACCCACCAGGAGCTGCGAGCACAGAAGGCCGCGGAGAATAGACGG
AGGACCGAGATGGTCCGGAAGCAGCGCGAGGAAGCTCGCCTAAAGAAGGA
GGCCGAACAGAGGGAGAGGGAAGCGAAGCAGCAAGAAGAGCACCGCCGGC
GAATCGAAGCCGAGAAAAAGAAGACACAAGAAGCAGAGCGCATGATTGGA
AAGATGGAGCAGGATGAGGACGTTTTGATCGAACGCCTCAAGAAGGTCCA
AGACGAGCAGCGTAAAGCGTACGAGTTGCTTCAGGAATCTCTTGAAAGCT
GA
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