mRNA_Ecto-sp13_S_contig180.5513.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: D8LSW0_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSW0_ECTSI)

HSP 1 Score: 1924 bits (4984), Expect = 0.000e+0
Identity = 972/1017 (95.58%), Postives = 994/1017 (97.74%), Query Frame = 2

Query:   35 MLVASSSPVLGVDLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEAAIADGEIAELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 3085
            MLVASSSPVLGVDLGKIQAIT SSAFDDFARSQSAYSAAS WY +R+S+DAAEGS APYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDF+LLSAAPLLPSLKLATSLLDHGPDTSP LGAAD+ DLSDHSAPLRSTYGEGMT+DN+HGLSVRLSPGVLTA G +SFAEAFLRDWHADLTSE+VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPL DAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGF FDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYL+DDTSYSSSSTSPSFTGGFHKDT+GGHGTWTAGSAAGAISA+S  ++EACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGGVAPGAQI+VFDVSYVGTE+YA  AGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIF+AGNDGGYEDIPNREACT+GSPALGKNSLAVGATSSGPFPGTRTGDDGNL YEELGLTDYSPEGYPWICL P+LGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSCKLVA MGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCA+HSSS QCSEFAPSAATVKAMIINSAHLMGGSSEPNG+RGFGRVHLEAGMPMDGVGETGL VVEAAIADGEI ELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHV+APSGAKYTMWESG DDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK
Sbjct:    1 MLVASSSPVLGVDLGKIQAITASSAFDDFARSQSAYSAASSWYNDRLSYDAAEGSTAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFDLLSAAPLLPSLKLATSLLDHGPDTSPRLGAADDFDLSDHSAPLRSTYGEGMTLDNVHGLSVRLSPGVLTAGGRESFAEAFLRDWHADLTSESVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFLFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLFDDTSYSSSSTSPSFTGGFHKDTVGGHGTWTAGSAAGAISAQSILEEEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISVFDVSYVGTEIYASFAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFAAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLVYEELGLTDYSPEGYPWICLTPFLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCKLVASMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAEHSSSCQCSEFAPSAATVKAMIINSAHLMGGSSEPNGVRGFGRVHLEAGMPMDGVGETGLSVVEAAIADGEITELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGVDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 1017          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: D8LU20_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LU20_ECTSI)

HSP 1 Score: 1604 bits (4153), Expect = 0.000e+0
Identity = 842/1035 (81.35%), Postives = 888/1035 (85.80%), Query Frame = 2

Query:    2 GFHTDLVAAFLMLVASSSPVLGVDLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDY----LGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSF--TGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEAAI-ADGEIAELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAIYSSK 3085
            G   D VAA L LVAS S V+GVDLGKIQ IT SSAFDDFARSQSAYSAAS W+T R++H A EGSRAPY+ACADYKQGRHA+TSLETAFT+SAVHRVSNTE DG+CFIVTALPSSATA+I APEDF LLSAAP LPSLKLAT LLDHGPDTSP +GAAD+ +LSD SAPLRSTYGE                                                         DRS+YDVTRVREWSRAAAVVD LASK A+T GEICGFGKL LRHVADDL+LVEGMNHLLPGK+ PSE RTACYMGLLSQLAAKPEVLRVSPLQSA L+NAVANALVQSATTTETPL DAGLDGTGEVIQVVD+GLDETSCFFADDDGLQVEHGF F GVRVE DGNVSTVLGNYSFPFDMSRRKIVQYIQ+FKTPDQGSTSSGQ+SFTY SDY    LGD +D DDSEY SYDDYSGEY SDTWSE+SFCA VLDDLSYHFS L+DDTSYS SS S S   TGGF KD +GGHGTWTAG AAGAISA+SS ++EACYGDELPGCAGGCISASDVDAMLD+ +FDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGGVAPGAQI++ D SY G++ +A  AGN LWYSAMETGAKIH+NSWG   LCQLTE+EYLYD+FMYENPEHLLIF+AGN GG EDIPN E CTIGSPALGKN LAVGATSSGP  GTRTG+DGNL YEE G+TDYSPEGYPWICL P LGTPSAS EPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTD HSC+L+A MGTSASCPLVAGAAALVRQYFKDPSFFAKDV SRGGCA+HSSS QCSEFAPSAATVKAMIINSAHLMGGSSEPNG+RGFGRVHLEAGMPMDGVGETGLLVVEA I +D EI ELVTVDGDAG ELRATLAWLDPPTTALSAKQLQHDLDLHV+APSGAKYTMWESG DDTVNVVERVIVPVESMESGVWSVVVSAK LLTDEQ YSLVVTGAIYSSK
Sbjct:    2 GLRADHVAAIL-LVASPSAVIGVDLGKIQTITASSAFDDFARSQSAYSAASKWHTKRLAH-APEGSRAPYIACADYKQGRHAMTSLETAFTESAVHRVSNTETDGSCFIVTALPSSATAMISAPEDFALLSAAPFLPSLKLATRLLDHGPDTSPHVGAADDFNLSDLSAPLRSTYGE---------------------------------------------------------DRSQYDVTRVREWSRAAAVVDSLASKRAQTFGEICGFGKLSLRHVADDLLLVEGMNHLLPGKKAPSEARTACYMGLLSQLAAKPEVLRVSPLQSASLYNAVANALVQSATTTETPLLDAGLDGTGEVIQVVDTGLDETSCFFADDDGLQVEHGFLFGGVRVEGDGNVSTVLGNYSFPFDMSRRKIVQYIQMFKTPDQGSTSSGQNSFTYASDYIRDYLGDAFDDDDSEYNSYDDYSGEYSSDTWSEESFCAQVLDDLSYHFSSLFDDTSYSPSSASSSISSTGGFDKDIVGGHGTWTAGCAAGAISAQSSLQEEACYGDELPGCAGGCISASDVDAMLDNGSFDLDVFCPMYNCDGNTDIPSSECLSDDPVENLHQHGGVAPGAQISMLDASYTGSDSFALFAGNMLWYSAMETGAKIHSNSWGYDMLCQLTENEYLYDSFMYENPEHLLIFAAGNHGGNEDIPNHEGCTIGSPALGKNILAVGATSSGPSRGTRTGEDGNLVYEEFGMTDYSPEGYPWICLSPSLGTPSASMEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDGHSCRLLANMGTSASCPLVAGAAALVRQYFKDPSFFAKDVTSRGGCAEHSSSSQCSEFAPSAATVKAMIINSAHLMGGSSEPNGLRGFGRVHLEAGMPMDGVGETGLLVVEATIGSDDEITELVTVDGDAGAELRATLAWLDPPTTALSAKQLQHDLDLHVIAPSGAKYTMWESGADDTVNVVERVIVPVESMESGVWSVVVSAKGLLTDEQSYSLVVTGAIYSSK 977          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A6H5JQ52_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQ52_9PHAE)

HSP 1 Score: 1099 bits (2842), Expect = 0.000e+0
Identity = 579/753 (76.89%), Postives = 605/753 (80.35%), Query Frame = 2

Query:  329 VTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTE---TPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRK-------------------------------------------------IVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQV-ASASSDGTDEHSCKL 2428
            V ALPSSATAVIRAPEDF+LLS  PLL SLKLATSLLDHGPDTSP LGAADNLDLSDHSAPLRSTYGEG                                          VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGM +LLPGKRFPSETRTACYMGLLSQLAAKPEVL VSPLQSA LHNAVANA +QSAT  +    P    G           DSGLD+TSCFFADDDGLQVEHG+ FDGVRVEDDGNVST LGNYSFPFDMSRRK                                                 IVQ IQLFKTP QGSTSSGQDSFT+ SD++GDP+DYDDSEYISY DYSGEYYSDTWSEDSFCAG LDDLSYH SYLYDDTSY+S STSPSFTGGFH+DTIGGHGT T+GSAAGAIS++SS K+EACYGDELPGCAGGCISASDVDAMLDDSTF L VFCPMY CDGN DIPSSECLSDDPVE+LHQHGGVAPGAQI+VFD SYVGTEVYA  AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YDTFMYENPEHLLIFSAGNDGGY+DIPNREACT+GSPALGKN LAVGATSSGPFPGTRTGDDGNLF EE+G+TDYSPEGYPWICLMP LG P ASTEPAGVDTV +FSSQGPTLDG+IKP VVAPG+QV ASASSDGTDEHSCKL
Sbjct:    5 VAALPSSATAVIRAPEDFDLLSVVPLLSSLKLATSLLDHGPDTSPRLGAADNLDLSDHSAPLRSTYGEG------------------------------------------VNMRRASFWSDSDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMANLLPGKRFPSETRTACYMGLLSQLAAKPEVLCVSPLQSATLHNAVANASMQSATYHDRNAAPRCWIGRHWRSHP----DSGLDKTSCFFADDDGLQVEHGYLFDGVRVEDDGNVSTALGNYSFPFDMSRRKVGKVSAPEPLGILGTGDITTPCDITDPREILEINHFDFQTFSHGGVISQIVQCIQLFKTPHQGSTSSGQDSFTFFSDFIGDPFDYDDSEYISYGDYSGEYYSDTWSEDSFCAGGLDDLSYHLSYLYDDTSYASPSTSPSFTGGFHQDTIGGHGTGTSGSAAGAISSQSSLKEEACYGDELPGCAGGCISASDVDAMLDDSTFHLVVFCPMYTCDGNIDIPSSECLSDDPVEDLHQHGGVAPGAQISVFDFSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDTFMYENPEHLLIFSAGNDGGYDDIPNREACTVGSPALGKNILAVGATSSGPFPGTRTGDDGNLFCEEVGVTDYSPEGYPWICLMPVLGAPPASTEPAGVDTVGYFSSQGPTLDGQIKPGVVAPGNQVVASASSDGTDEHSCKL 711          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A6H5KDF8_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KDF8_9PHAE)

HSP 1 Score: 688 bits (1776), Expect = 3.160e-234
Identity = 355/489 (72.60%), Postives = 375/489 (76.69%), Query Frame = 2

Query: 1223 YSFPFDMSRRKIVQYIQLF---KTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDT-------------------------------------------------W-----------------------------------SEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 2428
            +S P+  +   +V Y  +    KTPDQGSTSSGQDSFTY SDY GDPYDYDDSEYIS+DDYSGEY+SDT                                                 W                                   SE+    G LDDL+YH SYLYDDTSY+SSSTSPSFTGGFHKDTIGGHGT TAGSAAGAIS++SS ++EACYGDELP CAGGCISASDV+AMLDDSTFDLDVFCPMY CDGNTDIPSSECLSDDPVENLHQHGG APGAQI+VFDVSYVGTEVYA  AGNDLWYSAMETGAKIHTNSWGGATLCQLTE EY+YD FMYENPEHLLIFSAGNDGGYEDIPNREACT+GSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEE+GLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVA+FSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL
Sbjct:   15 FSTPYKCNGCNVVTYRAVHPAVKTPDQGSTSSGQDSFTYFSDYFGDPYDYDDSEYISHDDYSGEYFSDTVVPALKTLILSQLPAATDSRSTARVLTDNLFVEKASGLTDAEVRQAVKTWLSVEDDPEVLEEEIAMELQAVDEQLAGVTLDDDVPSEEEDDDGGLDDLAYHLSYLYDDTSYASSSTSPSFTGGFHKDTIGGHGTSTAGSAAGAISSQSSLEEEACYGDELPACAGGCISASDVEAMLDDSTFDLDVFCPMYTCDGNTDIPSSECLSDDPVENLHQHGGAAPGAQISVFDVSYVGTEVYASWAGNDLWYSAMETGAKIHTNSWGGATLCQLTEKEYMYDMFMYENPEHLLIFSAGNDGGYEDIPNREACTVGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEEVGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAYFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKL 503          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A6H5K229_9PHAE (Subtilisin n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5K229_9PHAE)

HSP 1 Score: 673 bits (1737), Expect = 1.790e-221
Identity = 432/1017 (42.48%), Postives = 577/1017 (56.74%), Query Frame = 2

Query:  107 AFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP--------SETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVST----VLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASD-----VDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLA--GNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEA---AIADGEIAE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 3073
            A DDF     A+ AA+ W   R+     +G+RAP++ CA+Y  GR   + L+   +  AV  VS++E  G CF+ TA  + A+A++   E F L S +P   +LKLA  L+DH  ++  + G       S     L + +G  M + N+ GL+V+L+P  L A      AE+F+ D   DL S +V++   +FWSD      E+        R R+WS+AA+VV  L+  +  + G+IC + ++ L    DD +LV G++HLL   R          +E   AC+MGL+S  A +PEVLRVSP  + R+ N  A  ++QSA+ T+TPL DAGLDGTGEVIQVVD+GLDETSCFF DD G +V+HG++F+ + V   G+       V     F +   RRK++QYI+L                              DS+Y+++    G  Y                      +L  D               F +D   GHGT TAGSAAGA   + +     C GDE  GC GGCI  S+     V   +  +  DLD  CPMY CDG+TD     CLSD+  E L ++GG+A GA++++FDV +   EV+  +   GN +W   +E G K+H+ S GG TLC   E E LYD FMYENPE+LLIF+AGNDG  E    R  CT+ SPA+GKN LAVGA++SG         D  +   E G                           AG+D VA FSS G T DGRIKP+VVAPGD V SA+SDGTD HSC+L AY GTS SCP+VAGAAA+VRQYF D +F+  DV +RG C   S  + C  F+PSAATVKA++INSA+LMG SS+P+  RGFGR+HLE GMP++G G+  L V +A   +IA+  + E L  VD DAG+ELRATL+W+D P T+ SA QL +DLDL VV+PSG  Y MW S   D  NV ERVIV  + +E+G W+V+V + +L+TD Q YSLVV GAI
Sbjct:   45 AKDDFNGHARAFEAAATWNGERLRRSQ-DGTRAPHLCCAEYGHGRETFSRLKQFLSPDAVRVVSHSEDHGACFLATASHAQASAIMEDQEQFGLESFSPFPSALKLAPGLVDHS-ESREVEGEP-----SSGQDRLGARHGGTMRMSNVEGLNVKLTPDTLPARSAG--AESFIIDLLGDLMSASVDLHSTNFWSDPVMAGGEHLSSHGGAVRARDWSKAASVVHELSEAAGTSPGDICSWDEVALIQAGDDSLLVTGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFFAGRPEVLRVSPRHTKRVLNEAARGIIQSASATDTPLTDAGLDGTGEVIQVVDTGLDETSCFFIDDGGEEVDHGYFFEELFVGSTGSSQLIYYPVFEAGDFTYTEERRKVIQYIELVNP---------------------------DSDYVAFSTTQGGVYP---------------------FLPPDE--------------FGQDDSAGHGTHTAGSAAGATLNDPAETLT-CSGDETLGCVGGCIDESETTDDLVSYAIQIALPDLDRLCPMYGCDGSTD----PCLSDEVDETLTKNGGMAQGAKLSIFDVFF---EVFGLIEVLGNGVWEPCLEAGCKVHSLSLGGDTLCTPGESEILYDDFMYENPENLLIFAAGNDGETE----RSVCTMASPAIGKNVLAVGASTSG---------DTRVISTEGG---------------------------AGIDMVADFSSYGFTTDGRIKPEVVAPGDMVYSAASDGTDSHSCRLWAYQGTSMSCPIVAGAAAMVRQYFVDETFYTADVTARGFC---SDGFICEGFSPSAATVKALLINSANLMGESSQPDAYRGFGRIHLEQGMPLEGEGDLALFVADALDTSIAEDTVQEYLFDVDADAGLELRATLSWIDYPATSTSAMQLVNDLDLVVVSPSGTVYRMWGSDVTDNRNVNERVIVDADDVETGTWTVLVYSNSLVTDSQSYSLVVNGAI 939          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: D8LNG6_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LNG6_ECTSI)

HSP 1 Score: 637 bits (1642), Expect = 1.100e-207
Identity = 424/1045 (40.57%), Postives = 564/1045 (53.97%), Query Frame = 2

Query:   26 AFLMLVASSSPVLG----VDLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDH--GPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDAD------RSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP--------SETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDV-DAMLDDST----FDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEA---AIADGEIAE-LVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 3073
            A L+ + SS+   G      L  ++   +++A DDF     A+ AA+ W   R+ H   E +RAP++ACA Y  G  A + L+   +  AV  VS++E  G CF  +A  + A A++   E F L + +P    LKLA  L+DH    D +     +  LD       L + +G  M   N+ GLSV L+PG L A   +  A++F+     DL S +V++   +FWSD   D       S     R R+W +AA+VV  L+  ++   G+IC + ++ + H  DD +LV G++HLL   R          +E   AC+MGL+S LA +PEVLRVSP  + R+ NA A  ++QSA+ T+TPL DAGLDGTGE+IQV+DSGLDETSC+F DD G +V+HG++FD +     G  +    +YS                    D+ +T      F+Y                                                                     + +    GHGT TAGSAAGA   + +        D L  C G CI   D  D ++  S+     DLD  CPMY CD +TD   + CLSDD  E L  +GG+A GA++A+FDV Y G +  + + GN+LW    E G K+H+NS G    C +   + L D FMYEN E+LLIF+AGN+GG E    R  CTI +P +GKNSLA+GA++SG    TR           +G T                          G+DTVA FSS G T D RIKP+VVAPGD V SA+SDGTD HSC+L AY GTS SCP+VAGAAA++RQYF D +F+  DV +RG C   S  + C  F+PSAATVKA++INSA+LMGGSSEP+G RGFGRVHLEAGMP++G G+  L V +A   +IA G + E L  VD DAG++LRATL+W+DPP TA SA QL H+LDL VV+P G  Y MW S   D  NV ERVIV    + +G W+V V A  L+TD Q YSLVV GAI
Sbjct:    9 ALLVFLCSSAVAAGRLNSPALELVENALLTTAKDDFKGHVGAFEAAATWNGERLRHSQEE-TRAPHLACARYGDGHEASSRLKQFLSPEAVRVVSHSEDHGACFFASASHAQAAAIVEDQEQFGLENFSPFPSPLKLAPGLVDHTESHDEAEEEARSSGLDR------LSARHGARMRKPNVEGLSVELTPGTLAARSSE--AKSFINHMLGDLMSASVDLHSTNFWSDPGMDGAGEHLSSPAGAVRARDWRKAASVVHELSEAASTGPGDICSWNRIFVHHAGDDSLLVSGLDHLLFSGRGAGGSHEEESTELHVACFMGLVSFLAGRPEVLRVSPRPTKRVLNAAARGVIQSASATDTPLTDAGLDGTGEIIQVIDSGLDETSCYFIDDSGEEVDHGYYFDEL-----GRAAVYSSSYS-------------------SDETATIFNGGDFSY---------------------------------------------------------------------YPERRKAGHGTHTAGSAAGATLNDPAETITCNVTDTLS-CVGACIDLLDATDDLVSISSQTDGTDLDRLCPMYGCDNSTD---TTCLSDDVPETLSNNGGMAQGAKLAIFDVFYGGYDASSSI-GNNLWEVCTEAGCKLHSNSLGADFECSIASWDILNDQFMYENAENLLIFAAGNEGGIE----RSTCTINTPGIGKNSLAIGASTSGD---TRV-------ISTVGAT--------------------------GIDTVAEFSSWGFTTDDRIKPEVVAPGDSVYSAASDGTDTHSCRLWAYEGTSMSCPIVAGAAAMIRQYFVDETFYTADVTARGFC---SDGFICEGFSPSAATVKALLINSANLMGGSSEPDGFRGFGRVHLEAGMPLEGEGDLVLFVADALDTSIAGGTLEEYLFDVDADAGLDLRATLSWIDPPATATSAVQLVHNLDLVVVSPDGTTYRMWGSDTTDNRNVNERVIVDATDVVTGTWTVWVWANNLVTDSQSYSLVVNGAI 903          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A6H5JQT4_9PHAE (Subtilisin n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JQT4_9PHAE)

HSP 1 Score: 611 bits (1576), Expect = 1.250e-196
Identity = 416/1046 (39.77%), Postives = 566/1046 (54.11%), Query Frame = 2

Query:  107 AFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEY-----DVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP---------SETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFA-DDDGLQVEHGFWFD--GV-----------------------RVEDD---GNVSTVLGNYS---FPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCIS---ASDVDAMLDDST--FDLDVFCPMYACDGNTDIPSSE-CLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGT-DEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEA---AIADGEIAEL-VTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGAI 3073
            A DDF     A+ AA+ W   R+ H   E +RAP++ CA+Y  GR   + L+   +  AV  VS++E  G CF  +A  + A+A+I   E F L S +P   +LKLA  L+DH             LD       L + +G  M + N+ GLS+ L+PG L +   +  AEAF+ D   DL   +V++   +FWSD +    E         R R+WS+AA+VV  L+  +  + G+IC + ++ + H   D++LV G++HLL   R           +E   AC+MGL+S LA+KPEVLRV+P QS  L NA A A +QSAT T+TPL DAGLDGTG+V+QV+DSG+DE+SCFFA D+ G  V HG +F+  G+                       R  DD   G+   +   +    F     RRKI+QYI L K+ D  +  S  DSFT     LG  Y +  ++   YD                               Y+                       GHGT  AGS AGA    +  +  +C   E   C G C+    A+DVD +   +    DLD  CP+++ D + D+  +E CLSDD  E L  +GG+A GA++A+FD  Y    +  ++ GN LW    E   KIH+NS+GG   C L  ++ LYD +M  NPE+LLIF+AGNDG       R  CT+ SPA+ KN LAVGA++SG                                      T  ++T   G+DTVA FSS GPT D RIKP+V+APGD + SA+SDG+ D HSC+L AY GTS S P+VAGA+A++RQYF + +FFA D+ +RG C + +    C  F+PS+AT+KA++INSA+LMGGSSEP+G RGFGR+HLEAGMP+ G G   L V ++   +I +    E    V+  A ++LR TL+WLDPP T  SA QL HDLDL V++P+G +YTMW SG  D+ NV ERVIV    +E+G WSV + A  L TD Q YSLVV GAI
Sbjct:   35 AKDDFNGHARAFEAAATWNGERLRHRQDE-TRAPHLCCAEYGHGRETFSRLKQFLSPEAVRVVSHSEDLGACFFASASHAQASAIIEDQEQFGLESFSPFPSALKLAPGLVDHSQRHDKDKETTTGLDR------LSARHGARMRMSNVEGLSIELTPGTLASRSVE--AEAFISDTLGDLMLASVDLHSTNFWSDQNMGGGEQLSFPGGAVRARDWSKAASVVHELSEAAGTSPGDICSWNRVSVHHAEGDVLLVSGLDHLLHSGRGAGGRSDGGESAELHIACFMGLVSFLASKPEVLRVAPRQSQALLNASARANIQSATVTDTPLTDAGLDGTGQVVQVIDSGVDESSCFFAHDESGDHVPHGHYFEEWGINPPLTSSSFSSPTSDDDDSLGWPRATDDHRTGSYYQIAEEFDGGDFTIFPDRRKIIQYINLIKSDD--TEGSHGDSFTTS---LGQQYPWLPADRFEYD-------------------------------YE----------------------AGHGTHVAGSVAGAT-LNTPAELISCKDTETMSCVGACVDDDEANDVDDLATPNVEIVDLDRLCPLFS-DYDCDVLETEKCLSDDVAETLTNNGGMAQGAKLAIFDAFYDNFGLSPFV-GNGLWEPCAEADCKIHSNSYGGDFECSLGPNDVLYDGWMVSNPENLLIFAAGNDGERR----RSTCTMSSPAIAKNVLAVGASTSGD-------------------------------------TRLSATPGLGIDTVAVFSSYGPTSDSRIKPEVLAPGDAIYSAASDGSMDSHSCRLWAYAGTSMSTPIVAGASAMIRQYFMNETFFATDMTARGFCDEWAG---CEAFSPSSATLKALMINSANLMGGSSEPDGSRGFGRIHLEAGMPLRGEGSLALFVSDSNYTSIPERTRQEYNFDVNATARLDLRVTLSWLDPPATTFSAVQLVHDLDLVVISPNGTRYTMWASGVTDSANVNERVIVDAGDVETGTWSVWIWADNLTTDVQSYSLVVNGAI 966          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: D8LQI1_ECTSI (Subtilisin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LQI1_ECTSI)

HSP 1 Score: 606 bits (1562), Expect = 2.140e-195
Identity = 411/1051 (39.11%), Postives = 559/1051 (53.19%), Query Frame = 2

Query:   23 AAFLMLVASSSPVLGV-DLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAP-LRSTYGEGMTVDNIHGLSVRLSPGVLTA-AGGDSFAEAFLRDWHADLTSETVNMRRASFWSD-----SDADRSEYDVTRVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFPS--------ETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRV---------EDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVDAMLDDSTFDLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEAA----IADGEIAELVTVDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWESGEDDTVNVVERVIVPVESME----SGVWSVVVSAKALLTDEQL-YSLVVTGAI 3073
            A  ++L+++ S    V  LG  +   +++A  DF  S++A++ AS W          +G  +PY++C ++ +GRHA   LE      AV  VS+T   G CF+VTA P+ A A + + EDF    A P   +LK+A  LLDHG    P          SD SA  L +T+G  M VD+ HGL V LSPG L + +GGD+ A  F+++    L+S ++++   + WSD      D   S     R REWSRAA +V+  +     T  ++CG+ ++ + H A+D++LV G++HLL   R  S        E R ACYMGL+S LA++ EVL+V+        NA A A +QSA+   TPL DAGLDG+GEVIQ++D+G++ET+CF    DG +VEHG++FD V           E             F +D  RRK++QYI L   PD G  SS    F     ++ D                                  DDL                                GHGT  A + AG+  +  +       G E+ GCAGGCI+ S   +   D   D++  CP + C G+      +CL DD  + L +HGG+A GA+IA  DV Y G   Y  LAGN LW +  + G KIH+NS+G    CQLT  + LYD +MYEN EHLL+FSAGN G   D   R+ CTI SP + KN L VG+TSSG                 L  TD    GY            +   E + +D V+ FS  G T DGRIKP+VVAPGD + SAS +G D  SC+L+AY GTS S  + +GAAA+VRQYF+D SF++ DV SRG C Q    + C  F+P A TVKAM+INSA++MGG +EP+  RGFGR+HLEAGMP+ G G+  L V +++     +D ++     VD DAG+ELRATL+W DP  ++LS  QL +DLDL V AP G +Y MW+    DT NV ERVIVP E +     +  W V V  +     + + +SLVVTGAI
Sbjct:    9 ATSVLLISTVSGASQVPSLGFFEQTLLTTAGADFD-SEAAFAFASAWQAGDR-----QGRSSPYLSCVEHGEGRHAYRKLEGFLGARAVKPVSSTATHGACFMVTASPAEA-AQLSSWEDFTTFGAFPA--ALKIAPGLLDHGSCDGPAAARDAATTTSDCSAGRLSTTHGASMQVDSAHGLMVELSPGTLPSHSGGDAKAHEFIQELLKSLSSPSLDLHAITCWSDLVMKGGDHLFSAAGALRGREWSRAATLVNERSESEQETPSQVCGWEQVVVHHAANDVLLVAGLDHLLYDGREVSGASEEEAAELRMACYMGLVSFLASRHEVLKVAGWNRKVNLNAAARATIQSASVDSTPLTDAGLDGSGEVIQIIDAGVNETTCFLEHGDGTEVEHGYYFDFVASSNPLFTSSSESRSMFKAEFTGGDFSYDSDRRKVIQYIDLIN-PDTGDGSSSGMPFVSADSFVSD----------------------------------DDL--------------------------------GHGTHAATTVAGSTISSPAELVSCADGSEVIGCAGGCITESSTGSSEADYGEDINRLCPAFGCSGD------QCLPDDTADTLSEHGGMAQGAKIAAIDVFY-GDYSYGDLAGNGLWETCADAGCKIHSNSYGVDLRCQLTALDVLYDEYMYENQEHLLLFSAGNTGWTTD---RDTCTIDSPGIAKNVLTVGSTSSG--------------VTRLSTTDLDGRGY------------ADGDELSTIDVVSEFSGYGFTEDGRIKPEVVAPGDMIYSASGNGGDS-SCELIAYAGTSFSTAVASGAAAMVRQYFRDSSFYSADVESRGFCDQ---GFTCDSFSPMATTVKAMLINSANVMGGGNEPDTERGFGRIHLEAGMPLGGAGDMALFVADSSDHEVYSDEKLKFYFNVDPDAGMELRATLSWNDPAASSLSGDQLVNDLDLRVYAPDGTRYCMWDDNCWDTSNVNERVIVPAEVVAQFSATDEWEVRVRGRDFTVGDTVSFSLVVTGAI 943          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A2I6U1R2_SACJA (Subtilisin (Fragment) n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1R2_SACJA)

HSP 1 Score: 591 bits (1523), Expect = 3.170e-191
Identity = 396/1035 (38.26%), Postives = 543/1035 (52.46%), Query Frame = 2

Query:    8 HTDLVAAFLMLVASSSPVLGV-------DLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHS-APLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGKRFP-------SETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPDQGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISAS------DVDAMLDD-STFDLDV--FCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEAAIADGEIAELVT------VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWE-SGEDDTVNVVERVIVPVESMESGVWS 3004
            H    A F  L A+S+P  G         L  ++   +++A D F     A+  A+ W    +     + +RAP++ C +Y +G   L  L+   +  AV  V+++   G CF VTA  + A A+  +P  + L S +P   +LK+A  +L+HG           +LD +  S   L +T+G  M   N+ GL++ LSPG L A   +  A  F+ +   DL S+++++   + WSD  A   E+  T     R REWS AA +V  LA     + G+IC +  + + +  +DL+LV G++HLL  +          +E R AC+MGL+S L+ + EVLRVSP  + RL NAVA  ++QSAT TE PL DAGLDGTG+VIQV+D+GLDETSCFF DDDG +V HG +++ +                        +I+            S+SS +D                                                                              + GHGT  AGSA GA     +     C G E+PGC GGCI A       D+  ++ + S +D+D+   CPM  CD  TD     CLSDD  + L  HGG+A GA++A FDV   G+    ++ GN LW   +E G KIH+ S G    C + E + LYD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG    T+T  DG+L     G  D                          VDTVA FSS GPT DGRIKP+VVAPGD + SA SDG+D HSC+L A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG      S  +C  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   L V +++     + EL +      VD +A ++ RATL+W+DPP ++ S  QL HDLDL VV+PSGA + MW  SGE D+ NV ERVI+  E +ESG ++
Sbjct:    5 HRTATALFFSLAAASTPWGGSANTLPYPSLSVVEGSLLTTAQDSFDGHAKAFETAAQWTGEILRSKEEDNARAPHLVCTEYNRGGKTLPRLQELLSPGAVRPVAHSSTHGACFFVTASHAEARAMSDSPY-YGLTSISPFPSALKVAPGVLEHGV----------SLDQASQSPGRLTTTHGHLMRKGNVEGLNMELSPGTLPAHSPE--AGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISMHYAGNDLLLVSGLDHLLHKENGAIGSDEEATELRVACFMGLVSFLSNRLEVLRVSPRHADRLLNAVARTIIQSATITEAPLTDAGLDGTGQVIQVIDTGLDETSCFFIDDDGEEVPHGHYYEALG-----------------------EIIP-----------SSSSARDD-----------------------------------------------------------------------------VEGHGTHCAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLFTLMPEYSPYDIDIDRLCPMIDCDDATD---PRCLSDDVGQTLADHGGMAQGAKLAFFDVFADGSSGTHFV-GNGLWEPCLEAGCKIHSMSIGADFECSVCEKDVLYDDFMYNNPENLVVVAAGNAGDTED--GRTVCTMYSPAIGKNALAVGATSSGETRITKTSADGDLIDGTNGYAD--------------------------VDTVAQFSSYGPTQDGRIKPEVVAPGDAIYSARSDGSDNHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYVADQAARGW--SDDSGRRCESFSPSSATVKALLINSANLMGGSSEPDGYRGFGRIHLEMGMPLDGEGSLALFVADSSYTS--LPELCSQDFIFDVDANANLDFRATLSWIDPPASSASGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAEDVESGTYT 879          

BLAST of mRNA_Ecto-sp13_S_contig180.5513.1 vs. uniprot
Match: A0A2I6U1S5_SACJA (Subtilisin n=1 Tax=Saccharina japonica TaxID=88149 RepID=A0A2I6U1S5_SACJA)

HSP 1 Score: 580 bits (1494), Expect = 3.810e-186
Identity = 399/1065 (37.46%), Postives = 541/1065 (50.80%), Query Frame = 2

Query:    8 HTDLVAAFLMLVASSSPVLGV-------DLGKIQAITVSSAFDDFARSQSAYSAASDWYTNRVSHDAAEGSRAPYVACADYKQGRHALTSLETAFTKSAVHRVSNTEADGTCFIVTALPSSATAVIRAPEDFNLLSAAPLLPSLKLATSLLDHGPDTSPLLGAADNLDLSDHSAPLRSTYGEGMTVDNIHGLSVRLSPGVLTAAGGDSFAEAFLRDWHADLTSETVNMRRASFWSDSDADRSEYDVT-----RVREWSRAAAVVDGLASKSARTVGEICGFGKLKLRHVADDLVLVEGMNHLLPGK-------RFPSETRTACYMGLLSQLAAKPEVLRVSPLQSARLHNAVANALVQSATTTETPLFDAGLDGTGEVIQVVDSGLDETSCFFADDDGLQVEHGFWFDGVRVEDDGNVSTVLGNYSFPFDMSRRKIVQYIQLFKTPD----QGSTSSGQDSFTYVSDYLGDPYDYDDSEYISYDDYSGEYYSDTWSEDSFCAGVLDDLSYHFSYLYDDTSYSSSSTSPSFTGGFHKDTIGGHGTWTAGSAAGAISAESSRKQEACYGDELPGCAGGCISASDVD-------AMLDDSTF--DLDVFCPMYACDGNTDIPSSECLSDDPVENLHQHGGVAPGAQIAVFDVSYVGTEVYAWLAGNDLWYSAMETGAKIHTNSWGGATLCQLTEDEYLYDTFMYENPEHLLIFSAGNDGGYEDIPNREACTIGSPALGKNSLAVGATSSGPFPGTRTGDDGNLFYEELGLTDYSPEGYPWICLMPYLGTPSASTEPAGVDTVAFFSSQGPTLDGRIKPDVVAPGDQVASASSDGTDEHSCKLVAYMGTSASCPLVAGAAALVRQYFKDPSFFAKDVVSRGGCAQHSSSYQCSEFAPSAATVKAMIINSAHLMGGSSEPNGIRGFGRVHLEAGMPMDGVGETGLLVVEAAIADGEIAELVT------VDGDAGVELRATLAWLDPPTTALSAKQLQHDLDLHVVAPSGAKYTMWE-SGEDDTVNVVERVIVPVESMESGVWSVVVSAKALLTDEQLYSLVVTGA-IYSS 3082
            H    A FL LVA+S+P  G         LG ++   +++A + F      +  A+ W  + +     + +RAP++ C +Y +G   L+ L+   +  AV  V+++   G CF VTA  + A A+   P  + L S +P   +LK+A  +L+HG               S     L +T+G  M   N+ GL++ LSPG L A      A  F+ +   DL S+++++   + WSD  A   E+  T     R REWS AA +V  LA     + G+IC +  + + H  +DL+LV G++HLL  +          +E R AC+MGL+S L+++ EV RVSP  + RL NAVA  ++QSAT T+ PL  AGLDGTG+VIQV+                                                       QYI L K         ST+SG +   +VS                                                                  GF  D +  HGT TAGSA GA     +     C G E+PGC GGCI A            M ++S++  D+D  CPM  CD   +     CLSD   + L  HGG+A GA+++ FD+ Y G  ++    GN LW   +E G KIH+ S+G    C + + + LYD FMY NPE+L++ +AGN G  ED   R  CT+ SPA+GKN+LAVGATSSG      T  DG+      G +D                          VDTVA FSS GPT DGRIKP+VVAPGD V SA SDG+D HSC+L A  GTS +CP+VAGA+A+VRQYF D SF+  D  +RG C    S  QC  F+PS+ATVKA++INSA+LMGGSSEP+G RGFGR+HLE GMP+DG G   L V +++     + EL +      VD +A ++ RATL+W+DPP ++ S  QL HDLDL VV+PSGA + MW  SGE D+ NV ERVI+  E++ESG ++V VS   LLTD Q YSLVV GA +YSS
Sbjct:    5 HRTATALFLSLVAASTPWGGSANSLPYPSLGVVEGSLLTTAHESFDGHAKGFETAAQWTGDIIRSKEEDNARAPHLVCTEYNRGGETLSRLQELLSPEAVRSVAHSSTHGACFFVTASHAEARAMSDNPY-YGLTSISPFPSALKVAPGVLEHGVSRD---------QASQSPGRLATTHGHLMRKGNVEGLNLELSPGTLPAHSPQ--AGMFIAELLDDLMSDSIDLHGGNVWSDPVAANGEHLTTPAGAVRKREWSMAATLVHQLAVAGRTSPGDICSWDSISIHHAGNDLLLVSGLDHLLHKESGAIGSDEEATELRVACFMGLVSFLSSRLEVSRVSPRHADRLLNAVARTIIQSATITKAPLTAAGLDGTGQVIQVI-------------------------------------------------------QYINLIKADSVSGPASSTTSGGNRVWWVS----------------------------------------------------------------PDGFQGDDVDDHGTHTAGSALGATIRTPAETPTTCSGTEVPGCVGGCIDADRTSWGDDLLTLMPENSSYVADIDRICPMLGCDEENE---PWCLSDGVEQTLADHGGMAQGAKLSFFDI-YDGNMLFEQFIGNGLWEPCLEAGCKIHSLSFGADLECAVRDTDLLYDDFMYNNPENLVVVAAGNAGEIED--GRTVCTMYSPAIGKNALAVGATSSGETRVGVTSADGDRADGTNGYSD--------------------------VDTVAVFSSYGPTQDGRIKPEVVAPGDAVYSARSDGSDVHSCRLSAKSGTSMACPIVAGASAMVRQYFMDESFYLADQAARGWCDD--SGRQCESFSPSSATVKALLINSANLMGGSSEPDGFRGFGRIHLEMGMPLDGEGSLALFVADSSYTS--LPELSSQDFVFDVDANANLDFRATLSWIDPPASSTSGVQLVHDLDLEVVSPSGATHIMWATSGEVDSRNVNERVIIAAENVESGTYTVRVSTNHLLTDSQNYSLVVNGAMVYSS 902