mRNA_Ecto-sp13_S_contig96177.21241.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig96177.21241.1
Unique NamemRNA_Ecto-sp13_S_contig96177.21241.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: D7FYA4_ECTSI (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FYA4_ECTSI)

HSP 1 Score: 88.6 bits (218), Expect = 1.420e-19
Identity = 43/44 (97.73%), Postives = 43/44 (97.73%), Query Frame = 1 
Query:    4 QDALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 135
            QDALDRANE MGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA
Sbjct:  304 QDALDRANEAMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 347
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A6H5KRX9_9PHAE (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KRX9_9PHAE)

HSP 1 Score: 87.4 bits (215), Expect = 5.550e-19
Identity = 42/44 (95.45%), Postives = 44/44 (100.00%), Query Frame = 1 
Query:    4 QDALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 135
            QDALDRANEVMGS+LTQ+EVKIHVVRDVAPNKPMLCLSFLAPDA
Sbjct:  377 QDALDRANEVMGSNLTQQEVKIHVVRDVAPNKPMLCLSFLAPDA 420
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A835Z8A4_9STRA (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z8A4_9STRA)

HSP 1 Score: 55.8 bits (133), Expect = 7.450e-8
Identity = 25/41 (60.98%), Postives = 31/41 (75.61%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAP 129
            DA+ RA   +G+ L   +VK+HVVRDVAP KPMLCL+F AP
Sbjct:  373 DAIARAAAALGAPLAAADVKVHVVRDVAPKKPMLCLTFRAP 413
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A7S2RAB3_9STRA (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RAB3_9STRA)

HSP 1 Score: 52.8 bits (125), Expect = 9.120e-7
Identity = 23/40 (57.50%), Postives = 29/40 (72.50%), Query Frame = 1 
Query:   10 ALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAP 129
            A+ RA E +G  L + EV+ H+VRDVAP KPM+CLSF  P
Sbjct:  415 AIKRAEEALGGPLKREEVQAHMVRDVAPKKPMICLSFRLP 454
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A150G7T0_GONPE (tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase n=1 Tax=Gonium pectorale TaxID=33097 RepID=A0A150G7T0_GONPE)

HSP 1 Score: 47.8 bits (112), Expect = 8.940e-6
Identity = 22/41 (53.66%), Postives = 25/41 (60.98%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAP 129
            D L +A   +G  L    V +H VRDVAPNK MLCLSF  P
Sbjct:   45 DILAKAERYLGGPLEPESVSVHTVRDVAPNKLMLCLSFRVP 85
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A8J5J8D7_9STRA (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Phytophthora aleatoria TaxID=2496075 RepID=A0A8J5J8D7_9STRA)

HSP 1 Score: 48.1 bits (113), Expect = 3.930e-5
Identity = 19/43 (44.19%), Postives = 29/43 (67.44%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 135
            D  +RA ++MG  L  ++   H+VRDVAP K M+C+SF  P++
Sbjct:  618 DVKERAEKIMGGELDPKQTSFHLVRDVAPKKVMVCISFQLPES 660
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A150G7S3_GONPE (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Gonium pectorale TaxID=33097 RepID=A0A150G7S3_GONPE)

HSP 1 Score: 47.8 bits (112), Expect = 5.270e-5
Identity = 22/41 (53.66%), Postives = 25/41 (60.98%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAP 129
            D L +A   +G  L    V +H VRDVAPNK MLCLSF  P
Sbjct:  249 DILAKAERYLGGPLEPESVSVHTVRDVAPNKLMLCLSFRVP 289
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A2D4BIH5_PYTIN (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BIH5_PYTIN)

HSP 1 Score: 47.4 bits (111), Expect = 7.340e-5
Identity = 20/43 (46.51%), Postives = 26/43 (60.47%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 135
            D  +RA  +MG  L     K H VRDVAP K M+C+SF  P++
Sbjct:  316 DVRERAERIMGGKLDPERTKFHQVRDVAPKKVMMCISFQLPES 358
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: TRM5_PHYIT (tRNA (guanine(37)-N1)-methyltransferase n=16 Tax=Phytophthora TaxID=4783 RepID=TRM5_PHYIT)

HSP 1 Score: 47.4 bits (111), Expect = 7.340e-5
Identity = 20/44 (45.45%), Postives = 27/44 (61.36%), Query Frame = 1 
Query:    4 QDALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA 135
            QD   RA ++MG  L       H+VRDVAP K M+C+SF  P++
Sbjct:  342 QDVKQRAEKIMGGELDPERTSFHLVRDVAPKKVMVCISFQLPES 385
[Close]
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Match: A0A507DYU7_9FUNG (tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Powellomyces hirtus TaxID=109895 RepID=A0A507DYU7_9FUNG)

HSP 1 Score: 47.4 bits (111), Expect = 7.350e-5
Identity = 20/41 (48.78%), Postives = 28/41 (68.29%), Query Frame = 1 
Query:    7 DALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAP 129
            D ++R   VMG+ +    +K+H VR+VAPNK MLC+SF  P
Sbjct:  421 DVVERVEAVMGARIGDNLIKVHNVRNVAPNKDMLCISFRLP 461
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig96177.21241.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 10
Match NameE-valueIdentityDescription
D7FYA4_ECTSI1.420e-1997.73tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarbox... [more]
A0A6H5KRX9_9PHAE5.550e-1995.45tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Ec... [more]
A0A835Z8A4_9STRA7.450e-860.98tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Tr... [more]
A0A7S2RAB3_9STRA9.120e-757.50tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Rh... [more]
A0A150G7T0_GONPE8.940e-653.66tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarbox... [more]
A0A8J5J8D7_9STRA3.930e-544.19tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Ph... [more]
A0A150G7S3_GONPE5.270e-553.66tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Go... [more]
A0A2D4BIH5_PYTIN7.340e-546.51tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Py... [more]
TRM5_PHYIT7.340e-545.45tRNA (guanine(37)-N1)-methyltransferase n=16 Tax=P... [more]
A0A507DYU7_9FUNG7.350e-548.78tRNA (guanine(37)-N1)-methyltransferase n=1 Tax=Po... [more]
back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig96177contigEcto-sp13_S_contig96177:298..432 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score89.4
Seed ortholog evalue4.8e-16
Seed eggNOG ortholog2880.D7FYA4
Preferred nameTRM5
Model size135
KEGG rclassRC00003,RC00334
KEGG koko:K15429
KEGG ReactionR00597
Hectar predicted targeting categoryno signal peptide or anchor
GOsGO:0000959,GO:0000963,GO:0001510,GO:0002939,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005759,GO:0005829,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0008168,GO:0008173,GO:0008175,GO:0008757,GO:0009019,GO:0009451,GO:0009987,GO:0010467,GO:0016070,GO:0016423,GO:0016740,GO:0016741,GO:0030431,GO:0030488,GO:0031974,GO:0032259,GO:0032501,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0046483,GO:0052906,GO:0070013,GO:0070525,GO:0070900,GO:0070901,GO:0071704,GO:0090304,GO:0090646,GO:0140053,GO:0140098,GO:0140101,GO:1900864,GO:1901360
Exons1
EggNOG free text desc.tRNA (guanine(37)-N(1))-methyltransferase activity
EggNOG OGsCOG2520@1,KOG2078@2759
EC2.1.1.228
Cds size135
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464226.865351-CDS-Ecto-sp13_S_contig96177:297..4321681464226.865351-CDS-Ecto-sp13_S_contig96177:297..432Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig96177 298..432 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig96177.21241.1prot_Ecto-sp13_S_contig96177.21241.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig96177 298..432 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig96177.21241.1

>prot_Ecto-sp13_S_contig96177.21241.1 ID=prot_Ecto-sp13_S_contig96177.21241.1|Name=mRNA_Ecto-sp13_S_contig96177.21241.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=45bp
YQDALDRANEVMGSSLTQREVKIHVVRDVAPNKPMLCLSFLAPDA
back to top

mRNA from alignment at Ecto-sp13_S_contig96177:298..432-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig96177.21241.1 ID=mRNA_Ecto-sp13_S_contig96177.21241.1|Name=mRNA_Ecto-sp13_S_contig96177.21241.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=135bp|location=Sequence derived from alignment at Ecto-sp13_S_contig96177:298..432- (Ectocarpus species13 EcNAP12_S_4_19m)
TATCAGGACGCCCTCGATCGAGCGAACGAAGTCATGGGATCCAGCCTCAC
CCAGCGGGAGGTTAAGATCCACGTTGTGCGGGACGTCGCTCCCAACAAAC
CAATGCTTTGCCTCTCCTTCCTAGCTCCCGACGCC
back to top

Coding sequence (CDS) from alignment at Ecto-sp13_S_contig96177:298..432-

>mRNA_Ecto-sp13_S_contig96177.21241.1 ID=mRNA_Ecto-sp13_S_contig96177.21241.1|Name=mRNA_Ecto-sp13_S_contig96177.21241.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=135bp|location=Sequence derived from alignment at Ecto-sp13_S_contig96177:298..432- (Ectocarpus species13 EcNAP12_S_4_19m)
TATCAGGACGCCCTCGATCGAGCGAACGAAGTCATGGGATCCAGCCTCAC
CCAGCGGGAGGTTAAGATCCACGTTGTGCGGGACGTCGCTCCCAACAAAC
CAATGCTTTGCCTCTCCTTCCTAGCTCCCGACGCC
back to top