mRNA_Ecto-sp13_S_contig9475.21089.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig9475.21089.1
Unique NamemRNA_Ecto-sp13_S_contig9475.21089.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: D7FIN5_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FIN5_ECTSI)

HSP 1 Score: 155 bits (393), Expect = 1.230e-43
Identity = 80/88 (90.91%), Postives = 85/88 (96.59%), Query Frame = 1 
Query:   19 QFKTHAYLFANSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            QFKTHAYLFAN+D+DGTNLKL TSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITA+TVA+K K+ L
Sbjct:  281 QFKTHAYLFANTDDDGTNLKLGTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAVTVAMKNKMEL 368
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A835ZBG0_9STRA (Sodium/calcium exchanger protein-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZBG0_9STRA)

HSP 1 Score: 110 bits (276), Expect = 3.430e-27
Identity = 56/90 (62.22%), Postives = 72/90 (80.00%), Query Frame = 1 
Query:   13 FVQFKTHAYLFANSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            F Q KTH++L+ + DE  + L +  + +GL  VT  VA+LSE LVD+IDGFTE+AHLSK+FVGLILLPIIGNAVEH+TA+TVA+K K+ L
Sbjct:  166 FFQLKTHSHLYGDEDEVRSELSMPGACIGLLTVTLAVAYLSELLVDSIDGFTEQAHLSKTFVGLILLPIIGNAVEHVTAVTVAMKDKMEL 255
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A7S4W0D3_9STRA (Hypothetical protein n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S4W0D3_9STRA)

HSP 1 Score: 106 bits (265), Expect = 3.470e-25
Identity = 51/88 (57.95%), Postives = 72/88 (81.82%), Query Frame = 1 
Query:   19 QFKTHAYLFANSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            Q KTHA+LFA+ DE+  +L    ++ GL +VTG++A +S+FLVD+IDGF E   +SK+F+GLI+LPI+GNAVEHITAI+VA+K K++L
Sbjct:  308 QIKTHAFLFADEDEEMASLPFWVAMAGLVIVTGVIAIMSDFLVDSIDGFCESTGVSKTFIGLIILPIVGNAVEHITAISVAMKDKMDL 395
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: B8C8B3_THAPS (Calcium/proton exchanger, calcium antiporter n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C8B3_THAPS)

HSP 1 Score: 97.4 bits (241), Expect = 4.720e-22
Identity = 51/93 (54.84%), Postives = 70/93 (75.27%), Query Frame = 1 
Query:   13 FVQFKTHAYLFANSDEDGTNLKLVT---SLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            F Q KTHAYLF    ++G  + L+    +L+GL V+T +V  LSEFLV +IDGF EE HL +SFVG+I+LP++GNAVEHI+A++VA K K++L
Sbjct:  173 FFQLKTHAYLFEG--DEGGEVALIPFSWALIGLVVITAIVTVLSEFLVGSIDGFCEEFHLGRSFVGVIILPVVGNAVEHISAVSVAYKNKMDL 263
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A7S2V7W0_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V7W0_9STRA)

HSP 1 Score: 97.1 bits (240), Expect = 9.000e-22
Identity = 51/91 (56.04%), Postives = 71/91 (78.02%), Query Frame = 1 
Query:   13 FVQFKTHAYLFANSDE-DGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            F Q +TH ++F +    +G  + L  +LVGLA++T  V+ LS++LVD+IDGFTEEA LSKSFVG+ILLPIIGN VEH+TA+++A+K K+ L
Sbjct:  204 FFQLRTHKHMFEDEXXXEGGGMSLSGALVGLALMTLAVSVLSDYLVDSIDGFTEEADLSKSFVGIILLPIIGNVVEHLTAVSMAMKDKMEL 294
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A7S2UU16_9STRA (Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UU16_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 1.080e-21
Identity = 52/91 (57.14%), Postives = 66/91 (72.53%), Query Frame = 1 
Query:   13 FVQFKTHAYLFANSDE-DGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            F Q KTH  LF + D+ D   +    +L GLA VT LV + S++LV +IDGFTEEA LSK+FVG+ILLPI+GN VEHI A+TVA+K K+ L
Sbjct:   69 FFQLKTHRSLFEDDDDNDEEPMSFWFALGGLAFVTVLVTFFSDYLVSSIDGFTEEAGLSKTFVGIILLPIVGNVVEHIAAVTVAMKDKMEL 159
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A1Z5KS18_FISSO (Ca2+:H+ antiporter n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5KS18_FISSO)

HSP 1 Score: 95.9 bits (237), Expect = 4.310e-21
Identity = 49/90 (54.44%), Postives = 69/90 (76.67%), Query Frame = 1 
Query:   19 QFKTHAYLFANSDEDG--TNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            Q  THA +F N  +D   T + +  +LVGL   T LVA+ S+FLV++IDGFTE + +S++FVG+ILLPI+GNAVEH+TA+TVA+K K++L
Sbjct:  291 QLHTHADIFENDGDDAPFTRITMPVALVGLLTTTLLVAFFSDFLVESIDGFTEASGISRTFVGIILLPIVGNAVEHVTAVTVAMKDKMDL 380
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A7S1USB3_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1USB3_9STRA)

HSP 1 Score: 94.0 bits (232), Expect = 2.680e-20
Identity = 47/89 (52.81%), Postives = 67/89 (75.28%), Query Frame = 1 
Query:   19 QFKTHAYLFA-NSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            Q KTH +LF  + DE G  L +V S+ GL + T L+   S++LV++IDGF   + +S++FVGLI+LPI+GNAVEHITA+TVA+K K++L
Sbjct:  397 QLKTHTFLFEEDGDEGGARLSMVISMSGLCITTLLITIFSDYLVESIDGFCSSSGVSRTFVGLIILPIVGNAVEHITAVTVAMKNKMDL 485
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A024UQH5_9STRA (Calcium/proton exchanger n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UQH5_9STRA)

HSP 1 Score: 93.2 bits (230), Expect = 3.040e-20
Identity = 51/104 (49.04%), Postives = 71/104 (68.27%), Query Frame = 1 
Query:   13 FVQFKTHAYLFANSDED--------------GTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            + Q KTHA+LF +S+ D               TN+ L +S++ L + T LV+ LSE+LV +IDGF   AH+SKSFVG+I+LPI+GNAVEH+TA+ VA+K K+ L
Sbjct:  226 YFQMKTHAHLFDSSENDLTADDEGIDDDTPDHTNMTLSSSVIVLGLATVLVSILSEYLVGSIDGFVASAHVSKSFVGIIVLPIVGNAVEHLTAVRVALKHKMEL 329
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BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Match: A0A7S1ZP93_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S1ZP93_TRICV)

HSP 1 Score: 92.4 bits (228), Expect = 3.120e-20
Identity = 47/88 (53.41%), Postives = 67/88 (76.14%), Query Frame = 1 
Query:   19 QFKTHAYLFANSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVDAIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNL 282
            Q  THA LF + D+  T +    +L GL VVT ++A LS++LV +IDGF  E+ +S++FVGLI+LPI+GNAVEH+TAI+VA+K K++L
Sbjct:  168 QLHTHADLFDDDDDTPTEMSSTAALTGLVVVTLVIAQLSDYLVGSIDGFCTESGVSRTFVGLIVLPIVGNAVEHVTAISVAMKDKMDL 255
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig9475.21089.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FIN5_ECTSI1.230e-4390.91Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A835ZBG0_9STRA3.430e-2762.22Sodium/calcium exchanger protein-domain-containing... [more]
A0A7S4W0D3_9STRA3.470e-2557.95Hypothetical protein n=2 Tax=Ditylum brightwellii ... [more]
B8C8B3_THAPS4.720e-2254.84Calcium/proton exchanger, calcium antiporter n=1 T... [more]
A0A7S2V7W0_9STRA9.000e-2256.04Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S2UU16_9STRA1.080e-2157.14Hypothetical protein (Fragment) n=1 Tax=Fibrocapsa... [more]
A0A1Z5KS18_FISSO4.310e-2154.44Ca2+:H+ antiporter n=2 Tax=Fistulifera solaris Tax... [more]
A0A7S1USB3_9STRA2.680e-2052.81Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]
A0A024UQH5_9STRA3.040e-2049.04Calcium/proton exchanger n=1 Tax=Aphanomyces invad... [more]
A0A7S1ZP93_TRICV3.120e-2053.41Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig9475contigEcto-sp13_S_contig9475:447..1213 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score158.7
Seed ortholog evalue1.4e-36
Seed eggNOG ortholog2880.D7FIN5
Preferred nameVCX1
Model size300
KEGG koko:K07300,ko:K08286,ko:K11699,ko:K12619,ko:K20404
KEGG TC2.A.19
KEGG Pathwayko03008,ko03018,ko04150,map03008,map03018,map04150
Hectar predicted targeting categorysignal anchor
GOsGO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0003674,GO:0005215,GO:0005451,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0006810,GO:0006811,GO:0006812,GO:0006813,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0007034,GO:0008150,GO:0008324,GO:0009987,GO:0015075,GO:0015077,GO:0015078,GO:0015079,GO:0015085,GO:0015291,GO:0015297,GO:0015298,GO:0015299,GO:0015318,GO:0015368,GO:0015369,GO:0015386,GO:0015491,GO:0015672,GO:0016020,GO:0016021,GO:0019725,GO:0022804,GO:0022821,GO:0022857,GO:0022890,GO:0030001,GO:0030003,GO:0031090,GO:0031224,GO:0034220,GO:0034486,GO:0042592,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044425,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0046873,GO:0048878,GO:0050801,GO:0051139,GO:0051179,GO:0051234,GO:0055065,GO:0055074,GO:0055080,GO:0055082,GO:0055085,GO:0060401,GO:0065007,GO:0065008,GO:0070588,GO:0070838,GO:0071804,GO:0071805,GO:0072503,GO:0072507,GO:0072511,GO:0098588,GO:0098655,GO:0098660,GO:0098662,GO:0098771,GO:0098805,GO:0098852,GO:0099516,GO:0140146,GO:1901660,GO:1902600
Exons2
EggNOG free text desc.calcium:proton antiporter activity
EggNOG OGsCOG0387@1,KOG1397@2759
EC2.7.7.48
Cds size300
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02000,ko03009,ko03016,ko03019,ko03021,ko03036
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464215.270823-CDS-Ecto-sp13_S_contig9475:446..5721681464215.270823-CDS-Ecto-sp13_S_contig9475:446..572Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig9475 447..572 -
1681464215.2873116-CDS-Ecto-sp13_S_contig9475:1039..12131681464215.2873116-CDS-Ecto-sp13_S_contig9475:1039..1213Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig9475 1040..1213 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig9475.21089.1prot_Ecto-sp13_S_contig9475.21089.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig9475 447..1213 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig9475.21089.1

>prot_Ecto-sp13_S_contig9475.21089.1 ID=prot_Ecto-sp13_S_contig9475.21089.1|Name=mRNA_Ecto-sp13_S_contig9475.21089.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=100bp
LFFFFVQFKTHAYLFANSDEDGTNLKLVTSLVGLAVVTGLVAWLSEFLVD
AIDGFTEEAHLSKSFVGLILLPIIGNAVEHITAITVAVKVKVNLRRVGG*
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mRNA from alignment at Ecto-sp13_S_contig9475:447..1213-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig9475.21089.1 ID=mRNA_Ecto-sp13_S_contig9475.21089.1|Name=mRNA_Ecto-sp13_S_contig9475.21089.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=767bp|location=Sequence derived from alignment at Ecto-sp13_S_contig9475:447..1213- (Ectocarpus species13 EcNAP12_S_4_19m)
CTTTTTTTTTTTTTCGTACAGTTCAAGACCCACGCCTATCTGTTCGCCAA
CAGCGACGAGGACGGCACGAACCTCAAGCTGGTCACGTCGCTCGTGGGCC
TGGCGGTGGTCACGGGCCTCGTGGCTTGGCTGTCGGAGTTTCTGGTGGAC
GCCATCGACGGCTTCACGGAGGAGGTATGCGCGTTTTTTGTCGCGAGGGT
ACCCGCACGTAGGTGCGTCGAACAAACAACATGTGTGGTATCCTCCGGCA
ACTTAATGGTTACCTCGGTCCCAGATGATGAGAGATGGTATTTGCGTTGA
TAGAAAAAAGTGATGGCGAGAAACGAGCGCGTCAATAGGTTGCACCGCAC
GTTTTGTCAACGAGACCGAAGCGAAGAAGACATCCATTCGTGGAGAACCT
GGACAAAGCCGCACCTCGAGCGTCAGCCTTGGGTGTGTTGGCAATTCGTT
CGGACTGGTCCTTGTACGATGTTCAGGTCGGTAGATTGCTTCCCTGTAGC
CGGTAGATTCGCCGCTGGTGGCATGCTTCTGCGATGCTTTATCCCCTCTC
TTGGAGATCCCCCCCACCCCCCTGCTGTGCTGCACGGCATTTACTGACCC
TGCTCTTTTATTCCTACGGCCGCCACGCTTGGTGCGAAAAGGCACACCTG
TCCAAGTCGTTCGTGGGCCTGATCCTGCTGCCAATCATCGGGAACGCTGT
GGAGCACATCACGGCCATCACGGTGGCCGTGAAGGTAAAAGTGAATCTTC
GGCGTGTTGGGGGTTAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig9475:447..1213-

>mRNA_Ecto-sp13_S_contig9475.21089.1 ID=mRNA_Ecto-sp13_S_contig9475.21089.1|Name=mRNA_Ecto-sp13_S_contig9475.21089.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=300bp|location=Sequence derived from alignment at Ecto-sp13_S_contig9475:447..1213- (Ectocarpus species13 EcNAP12_S_4_19m)
CTTTTTTTTTTTTTCGTACAGTTCAAGACCCACGCCTATCTGTTCGCCAA
CAGCGACGAGGACGGCACGAACCTCAAGCTGGTCACGTCGCTCGTGGGCC
TGGCGGTGGTCACGGGCCTCGTGGCTTGGCTGTCGGAGTTTCTGGTGGAC
GCCATCGACGGCTTCACGGAGGAGGCACACCTGTCCAAGTCGTTCGTGGG
CCTGATCCTGCTGCCAATCATCGGGAACGCTGTGGAGCACATCACGGCCA
TCACGGTGGCCGTGAAGGTAAAAGTGAATCTTCGGCGTGTTGGGGGTTAA
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