mRNA_Ecto-sp13_S_contig8330.19693.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: D8LE29_ECTSI (Thiaminase II n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LE29_ECTSI)

HSP 1 Score: 890 bits (2300), Expect = 0.000e+0
Identity = 484/608 (79.61%), Postives = 490/608 (80.59%), Query Frame = 2

Query:  170 MDGMRKDVGHGFTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFGGE 1993
            MDGMRKDVG G TLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFL+AFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN                                             ELR+AFPNW ESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGG+QG S QPGG+DAG RGRF GVHERLALLCVDFDDTLTEGDT SLLVETAKAQRETPEDRVDLGREW LLTRTFL KWSE IEDSLSTK STT CTTNGSGAVDREGLELMLRKL VVDLDSVSRVSDSK                                                   DVISRVLSETLL+SHSS SSTTGIDA G VWAGETASPRLEG CPASVSSNDLVFDEHGVSTGEI VKIPGSFGKHQRF+DLAA ARESVASSES PRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGT ELPRTGAPSGSGQCVYRASQWAEIDVFLFGGE
Sbjct:    1 MDGMRKDVGKGSTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN---------------------------------------------ELRHAFPNWTESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGRQGMSAQPGGQDAGGRGRFRGVHERLALLCVDFDDTLTEGDTISLLVETAKAQRETPEDRVDLGREWGLLTRTFLGKWSETIEDSLSTKLSTT-CTTNGSGAVDREGLELMLRKLGVVDLDSVSRVSDSK---------------------------------------------------DVISRVLSETLLDSHSSSSSTTGIDAGGCVWAGETASPRLEGDCPASVSSNDLVFDEHGVSTGEILVKIPGSFGKHQRFLDLAATARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTGELPRTGAPSGSGQCVYRASQWAEIDVFLFGGE 511          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A6H5J5C1_9PHAE (TENA_THI-4 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J5C1_9PHAE)

HSP 1 Score: 811 bits (2094), Expect = 5.260e-288
Identity = 465/668 (69.61%), Postives = 482/668 (72.16%), Query Frame = 2

Query:  179 MRKDVGHGFTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN---------------TP----------------PPVKATAEYVDFLMAV--ASDPTSSVAQVLAAMVPCMRLYA-------------------------------FLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFGG 1990
            MR DVG  FTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFL+AFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVAN               TP                  VK  +++   L  +   +      A+ L       R +A                               F+ QELR A PNW ES YAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFAL RKAMELEFAFFDAQLDG TEAAMLEQSVGAEAGG+QG SVQPGGKDAG RGRF GVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSE IEDSLSTK STTACTTNGSGAVDREGLELMLRKLEVVDLDS SRVSDSKV R L R+                   + R    A++   +                                          GETASPRLEG CPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRF+DLAAMARESVASSES PRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFE+VARAFGIDI PLASVYEAMDG  ELP TGAP GSGQ VYRASQW EIDVFLFGG
Sbjct:    1 MRMDVGERFTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLRAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANVRAKTTGVKLQIGSCTPLNFGLTGVWNVVHCRFASVKLHSDWAHLLYPINQGNKQMQVSAKELGERAMKRRSFAGLYLASLQIGLYRRQFHQCNVMTFADVLSSLFVAQELRDALPNWTESPYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALCRKAMELEFAFFDAQLDGVTEAAMLEQSVGAEAGGRQGMSVQPGGKDAGGRGRFRGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSETIEDSLSTKLSTTACTTNGSGAVDREGLELMLRKLEVVDLDSASRVSDSKV-RELARE-------------------IRRYFLAAIIVQREP-----------------------------------------GETASPRLEGDCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFLDLAAMARESVASSESSPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFERVARAFGIDILPLASVYEAMDGRGELPTTGAPLGSGQRVYRASQWTEIDVFLFGG 607          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A0G4GYE5_VITBC (TENA_THI-4 domain-containing protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4GYE5_VITBC)

HSP 1 Score: 245 bits (626), Expect = 1.550e-68
Identity = 197/605 (32.56%), Postives = 284/605 (46.94%), Query Frame = 2

Query:  221 LWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAF--PNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGG-KDAGDRGRFHGVHER--LALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPAS--VSSNDLVFDEHGV-STGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVV----GNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFGGE 1993
            LWQ C+  A++SL+HPW L L     +  S+  ++ QDA+FL+ FAKAYA+AL K    D E IRAF++LIG VL EL LH S S +W ID  +T  P +AT  Y  FL  V +D  ++ A VLA+M PCM LYAF+G+ LR        A   Y EW+ TYSS +F   A  VE LLD+ +       L +L  +  E      DA    G        ++  E G     ++ P     +    R  G+ +R  L+ + +DFD T T+ DTT LL E A      P+    + + W  +   F  K+   + + L            G       GLE  L  L   +  +V+ VS++++L+G TRD+V   +   LR  W+VR      +S  L       ++S++WSQD+I  +L +                                 G P S  ++ N   F E    +TGEI+  + G+  K + F  L    RE       L    +V++GDSVTDLLA+L+AD+GIV+      + + +KV   +GID++PL          +E       S     +Y A  W EI V LFG E
Sbjct:   53 LWQKCRRRALKSLYHPWVLRLGASVLARESYVEFMRQDAFFLEIFAKAYAHALCKCPPGDVEGIRAFNALIGGVLSELTLHQS-STEWAIDT-STFTPNEATVRYTSFLRDVGADQWTTTAMVLASMAPCMSLYAFIGKALREGVGEEGMAGHRYNEWLATYSSAEFAQDAARVEELLDRYT-----DPLLSLSGQVGE------DAVR--GNLLYYYGNAMDLEYGFFDAQALPPPSCAPSASPSRHPGLAQRGVLSFVAIDFDGTCTQRDTTPLLHEVAAEVHSDPDQ---VRQRWRAIEDAFFDKYRRKVGELL-----------EGKVRDGMNGLEAFLGGLGEFEKAAVADVSEARLLQGFTRDDVRRMVKTSLRQKWQVRDACGEVLSGLLSHDVPASIISVSWSQDMIHTILQDAC-------------------------------GAPLSLPITCNSFSFREDDQRATGEISSHMTGAVDKLRAFRRL----REEKGREGRL----SVFIGDSVTDLLALLEADIGIVLKDALNKNNTLDKVISLYGIDVQPLVRAAMIAQCGQEAATVTPVSMPPMTIYAADGWDEIGVMLFGNE 589          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A2V0PBE0_9CHLO (Thiamine phosphate synthase n=1 Tax=Raphidocelis subcapitata TaxID=307507 RepID=A0A2V0PBE0_9CHLO)

HSP 1 Score: 253 bits (647), Expect = 4.370e-68
Identity = 200/611 (32.73%), Postives = 283/611 (46.32%), Query Frame = 2

Query:  200 GFTLPGS----LWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDE--EDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETA---KAQRETPED----RVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHS-SLSSTTGIDARGRVWAGETASP-RLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFG 1987
            G  LPG     LW +CQLEA R+L HP+ L L  G     +F++YVAQDA+FL  FA+AYA AL K  D   E       L+  V  EL LH SY+  WG+++        A   Y DFLMA A DP ++VA+VLA+MVPC RLY FLG  L  A P    + YA+WI  YSS ++       E+LLD+ +  A Y  L +LY++AM+LE AFF                           S  PG   A            + +L  DFDDT TE DT   L+  A     +R  PE+    R  L      L   + SK +  + + L    S  A      G  D  GL   + +L   D+   +   +S +L GL+RD+V ++          ++PG    ++ AL +   THVVS+NWS++ ++  L     ++     + + G +A   V AG    P RL      ++ +N+L     G+STG +   +  +  K + F  L A   +             V+VGDS TD+L +L AD GIVVG++    +     G  + PL +    +          A  G  + VY A+ W EI  FLFG
Sbjct:    9 GLPLPGGVSERLWGECQLEAFRALQHPFVLALGLGTLPRDTFRHYVAQDAHFLHFFARAYAAALDKCADANAEAHVVLQQLLMGVHMELRLHGSYARTWGVELDALDHVDPACQAYTDFLMATAEDPETTVAEVLASMVPCSRLYGFLGCALARAHPRQRGNPYADWISMYSSFEYLVHPMQKEALLDRLAGDAPYDRLRSLYKRAMQLEVAFF---------------------------SAAPGIPAA----------RHVGMLVCDFDDTCTEKDTIGTLLSAAIDAAVKRAGPEEXXXLRARLDAVVARLVENYSSKHAALLAEILPEGASAAAA----EGGFDEAGLRAFVERLSDFDVQMNAVAVESGILAGLSRDDVAAAARAVA-----LKPGCREALAAALGAELPTHVVSVNWSREFVAAALGLPAADAADWGAAGSDGDEAAAAVIAGAAPPPGRL------AIHANELARGADGLSTGALRRAVQCARDKGRLFAALQAARGKG-----GXXXXAAVFVGDSATDVLPLLAADYGIVVGSNKLLRRALARHGASLVPLCAA--PLTAPARGGGGAASDGVARVVYEAASWDEIHAFLFG 560          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A7J7QPC3_9CHLO (Thiamine phosphate synthase n=1 Tax=Scenedesmus sp. NREL 46B-D3 TaxID=2650976 RepID=A0A7J7QPC3_9CHLO)

HSP 1 Score: 250 bits (638), Expect = 1.050e-66
Identity = 201/630 (31.90%), Postives = 287/630 (45.56%), Query Frame = 2

Query:  188 DVGHGFTLPGSL----WQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVL----EELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPED------------RVDLGREWVLLTRTFLSKWSEAIEDSLST---KPSTTACTTNG--SGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSG-----QCVYRASQWAEIDVFLFG 1987
            D+  G  +PG L    W+D Q EAM +LHHP+   LA G     + K+YV QD +FL  FA+AYA A+ K      + A  +++  +L     EL LH SY+AKWG+ V     P  AT+ Y D+LM VA+DP +SVA+VLAAM+PC RLY FLG +L  A       AY+EW+ETYS  ++  +  + E L DK +    Y  L  LY +AM+LE  FF AQ +G                        P  +  G             LL  DFDDT+TE DT  +L+  A                     R  L  +   L   +++K  + +   L     KP+     TN   +   D++GL      L   D++    V D+  L GLT+ +V ++  G       +RPG  R +S A  +     V+S+NWS+ ++  VL  T               A                     + +N+L +   GVSTG++  ++     K  R +D  A+ ++  +SS S  R   VYVGDS +D+L +L AD GIV+G +    +   AFG+ ++PL S           P T A S          +Y AS W EI  FLFG
Sbjct:   56 DMFSGLPVPGGLSEQLWRDTQAEAMEALHHPFVQALAAGTLDRWA-KHYVLQDLFFLTRFARAYAAAIDKCAHSPHLGAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSAYTDYLMEVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATRGQPRHAYSEWVETYSGAEYLASPVIKEELFDKIAHAVPYGRLKQLYARAMQLEVNFFSAQPNG------------------------PAPRHVG-------------LLVCDFDDTITEKDTIGVLMAAAVEANVKSSXXXXXXXXXXXXLRQQLNAQVKTLADNYMTKQQQLLASLLPADDGKPAAAMSVTNSVNNKTYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKADVAAAAGGIA-----LRPGSMRTLSAAAAAGLPVAVLSVNWSRSLMEAVLQPT---------------AAAXXXXXXXXXXXXXXXXXLEIYANELAWGPEGVSTGDLRREVQCGRDKG-RVMD--ALLKQHQSSSSS--RGAVVYVGDSSSDILPLLKADYGIVIGGNRLLHRALEAFGVSLQPLCSA----------PMTPASSXXXXXXXXSVLYAASSWQEIAAFLFG 612          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A383WBI9_TETOB (Thiamine phosphate synthase n=2 Tax=Tetradesmus obliquus TaxID=3088 RepID=A0A383WBI9_TETOB)

HSP 1 Score: 250 bits (638), Expect = 1.140e-66
Identity = 195/629 (31.00%), Postives = 277/629 (44.04%), Query Frame = 2

Query:  188 DVGHGFTLPGSL----WQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVL----EELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETA------------KAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAV-----DREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARES---VASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASV-YEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFG 1987
            D+  G  +PG L    W D Q EAM +LHHP+   LA G    A F++YV QD +FL  FA+AYA A+ K      + A  +++  +L     EL LH SY+AKWG+ V     P  AT++Y DFLM VA+DP +SVA+VLAAM+PC RLY FLG +L  A       AY+EW+ TYS  ++  +  + E L DK +A   Y  L  LY +AM+LE  FF AQ +G                        P  +  G             LL  DFDDT+TE DT   L+  A                     R  L  +   L   +++K  + +   L      +A     S +V     D++GL      L   D++    V D+  L GLT+  V ++      S+  +RPG AR +S A  +     ++S+NWS+ ++  VL                                        + +N+LV+   GVSTG++  +I  +  K +    L    R S               VYVGDS +D+L +L AD GI++G++    K   AFG+ ++PL S                     G  +Y AS W EI  FLFG
Sbjct:   29 DIFSGLPVPGGLSERLWADSQAEAMEALHHPFVQALAAGTLDRACFQHYVLQDLFFLTRFARAYAAAIDKCGTTPALAAAKAVLAELLLGVDAELKLHGSYAAKWGVAVDEWVWPSAATSQYTDFLMQVATDPETSVAEVLAAMIPCARLYGFLGCQLAAATQGLPRHAYSEWVATYSGAEYLASPVMKEELFDKIAAAEPYDRLKQLYTRAMQLEVNFFSAQPNG------------------------PAPRHVG-------------LLVCDFDDTITEKDTIGTLLGAAVEXXXXXXXXXXXXXXXXXXXRQQLNTQVKNLADNYMTKQQQLLASLLPADDDASAAAAGVSNSVTNKSYDKDGLLAFCEALSDFDVEMNQVVVDAGCLAGLTKAGVIAAA-----SNITLRPGSARTLSAAAAAGLPVALLSVNWSKSLMQAVLQPAAXXXX--------XXXXXXXXXXXXXXXXXXXXXXMEIHANELVWGPDGVSTGDLQREIQCARDKGRVMDTLLQQHRSSSXXXXXXXXXXXXAVVYVGDSSSDILPLLKADYGIIIGSNKLLRKALAAFGVGLQPLCSAPMTPSSXXXXXXXXXXXXXEGPVLYEASSWQEIAAFLFG 607          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A2K3DGU0_CHLRE (Thiamine phosphate synthase n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A0A2K3DGU0_CHLRE)

HSP 1 Score: 239 bits (610), Expect = 3.450e-63
Identity = 213/625 (34.08%), Postives = 280/625 (44.80%), Query Frame = 2

Query:  209 LPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGID--VANTPPPVKATAEYVDFLMAVAS--DPTSSVAQVLAAMVPCMRLYAFLGQELRYAF-PNWAESA-----YAEWIETYSSEDFEDAAGLVESLLDKNSA-GADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTK--PSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGC--PASVS--SNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDL----------AAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRA-SQWAEIDVFLFGGE 1993
            L   LW + Q E   SLH P+  G+A G     +F++YVAQDAYFL  FA+AY  AL+KA   DE+       L+  V EEL LH SY+AKWG+D  VA T P   AT  Y DFLM VA       SVA++LAAM+PC RLY FLG  L  A  P   + A     Y EW++TYSS+++    GL E + ++ +  GAD   L ALYR+AM+LE  FF AQ                                      +     R + L VDFD+T T  DT   L+  A A    P         WV  T   L+    A +  L  +  P      T      D  GLE +L  L   D      V +  +LRG T  EV ++ AG      ++RP     ++ AL      HV+S+NWS   +   LS                    R+ AGE            PA+V    N L  D  G++TG +  ++  +  K     +L          AA A  S  S  S      VYVGDS +DL AMLD+D G+V+G++    +V R FG+ I PLA+          L     P+G+   +Y A   WAEI  FLFG E
Sbjct:   15 LAKELWDNVQHEVFMSLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDEQTFEDLTGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSA-ATKAYTDFLMDVADAGGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPCHDYWEWVKTYSSQEYLSVPGLKERMFERLAGKGADKAKLLALYRRAMQLEAEFFAAQ-------------------------------------PYSPPPRRFSALVVDFDETCTARDTIGGLMRLAAAAAGQPGRATAGDAAWVSATSARLADAYVAAQGELLGRLLPEGAPDAT----CYDAAGLEALLAALSDFDAAMNGPVEECGILRGSTAAEVAAAGAG-----LQLRPQCREVLAGALGRGIPVHVLSVNWSDVYVGAALSAAAXXXXXXXXXXXXXXLPVRL-AGEXXXXXXXXXXXGPAAVELLCNSLQLDGSGLTTGALTKRVQSARDKRAALAELRARLPAASTAAAAADGSTDSGSSSNPAALVYVGDSTSDLGAMLDSDFGVVIGSNALLRRVCRRFGVRILPLAAA--------PLDPRRVPAGT---LYEAVGGWAEIRAFLFGVE 580          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: U5D054_AMBTC (Uncharacterized protein n=2 Tax=Amborella trichopoda TaxID=13333 RepID=U5D054_AMBTC)

HSP 1 Score: 229 bits (585), Expect = 5.200e-63
Identity = 193/632 (30.54%), Postives = 285/632 (45.09%), Query Frame = 2

Query:  224 WQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVAS----------------DPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNS---AGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETA-----KAQRETPEDRV------DLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQ------------THVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLAS--VYEAMDGTEELPRTGAPSGSGQCVYRASQWAEIDVFLFG 1987
            W     E++ + + P+ + LA G   L +F++YVAQDA+FLK FA+AY  A   A+D++   +   L   VLEEL +H SY+ +WG+D+     P  AT +Y+DFLMA A+                + T   A  + AM PCMRLYAFLG+EL+  F N A+  Y +WI+ YSS+ FE AA  +E LLDK S    G +   +  LY +AM+LE  FF AQ         L                             H   E+  ++  DFD T T  D++++L E A     K ++   E  +      DL   W  L++ +  ++ + +ED +   PS  A         D +GL   L  L   +  + SRV++S VL+GL  +++              R G    +     S FQ             HV+S  W  D+I    S  LL+                                 S+ SN+ ++ E  VSTG I  ++     K + F D+     ES+   +   + ++VY+GDSV DLL +L+ADVGIV+G+S S  +V   FG+   PL S  V +  D  E+   +    G    +Y  S WAEI  F+ G
Sbjct:   14 WVKFLRESVFASYSPFIVCLAAGNLELDTFRHYVAQDAHFLKCFARAYEMAEDCADDDDAKASICELRKGVLEELKMHESYAEEWGVDLVKESTPSSATTKYIDFLMATAAGKVEGGKGPSRIATPFEKTKIAAYTVGAMTPCMRLYAFLGKELQ-RFVNSADHQYKKWIDIYSSDSFEVAALQIEELLDKLSISLTGEELEVIERLYHQAMKLEIEFFSAQPISQKTLVPLSN--------------------------VHKPSEQRLIIFSDFDLTCTVVDSSAILAEIAIRTAPKVEQNGSEQPIARQSSADLRNSWGALSQQYTEEYEQCVEDIV---PSDEA------EVFDYQGLCKALEHLSEFEKRANSRVNESGVLKGLNLEDIK-------------RAGERLILQDGCTSFFQQIVKRKDELNADVHVLSYCWCGDLIRSAFSSGLLD-------------------------------VLSIHSNEFIY-EGSVSTGGIVREVESPMDKSRVFKDVL----ESIGEPD---KRVSVYIGDSVGDLLCLLEADVGIVIGSSSSLRRVGSKFGVSFVPLLSGVVKKQRDFLEDSCSSSNWKGLSGTLYTVSSWAEIHAFILG 557          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A836B2K3_CHLIN (Thiamine phosphate synthase n=1 Tax=Chlamydomonas incerta TaxID=51695 RepID=A0A836B2K3_CHLIN)

HSP 1 Score: 238 bits (607), Expect = 7.700e-63
Identity = 218/642 (33.96%), Postives = 290/642 (45.17%), Query Frame = 2

Query:  140 PAPGRKSPRVMDGMRKDVGHGFTLPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAE--DEEDIRAFHSLIGSVLEELGLHASYSAKWGID--VANTPPPVKATAEYVDFLMAVAS--DPTSSVAQVLAAMVPCMRLYAFLGQELRYAF----PNWAESA--YAEWIETYSSEDFEDAAGLVESLLDKNSAG-ADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLG-REWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMSRALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARG-RVWAGETASPRLEGGCPASVSSNDLVFDEHGVSTGEIAVKIPGSFGKHQRFVDL--------AAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAMDGTEELPRTGAPSGSGQCVYRA-SQWAEIDVFLFGGE 1993
            PAPG  +    DG+ K+          LW + Q E   SLH P+  G+A G     +F++YVAQDAYFL  FA+AY  AL+KA   D+        L+  V EEL LH SY+AKWG+D  VA T P   AT  Y DFLM VA       SVA++LAAM+PC RLY FLG  L  A     P+ A  A  Y EW+ TYSS D+    GL E + ++ +   AD   L  LYR+AM+LE  FF AQ                                      +     R + L VDFD+T T  DT   L+  A A     + R   G   WV  T + L+    A +  L  +          + A D  GL+  L  L   D    + V    +LRG T  EV ++ AG      ++RP     +S AL      HV+S+NWS   +   L+     +           A   R+   E A P +E  C      N L  D  G++TG +A ++  +  K     +L        AA A +  + S S      VYVGDS +DL AMLD+D G+V+G++    +V R FGI I PLA+          L     P+G+   +Y A   WAEI  FLFG E
Sbjct:    5 PAPGLPA---TDGLAKE----------LWDNVQHEVFMSLHDPFVQGMAQGTLDKRAFQHYVAQDAYFLHYFARAYGIALSKALALDDSTFEDLSGLLRGVHEELQLHGSYAAKWGVDMTVAGTRPSA-ATKAYTDFLMNVADAEGQDGSVAEILAAMLPCARLYGFLGAALAGAHGPQAPDQAPPAHHYWEWVRTYSSADYLSVPGLKERMFERLAGKEADKAKLLRLYRRAMQLEAEFFAAQ-------------------------------------PYSPPPRRFSALVVDFDETCTAKDTIGGLMRLAAAAAG-QQGRATAGDAAWVAATSSRLAADYVAAQGELLGRLLPEGAPD--AAAYDAAGLDAFLAALSDFDAAMNAPVEACGILRGSTSAEVAAAGAG-----LQLRPQCREVLSGALGRGIPVHVLSVNWSDVYVGAALAAPATAAVXXXXXXXXXSALPVRLAGAEGAGPAVELRC------NSLELDGAGLTTGALAKRVQSARDKRAALAELRSRLPAAPAAAAVDGASDSGSDNPAALVYVGDSTSDLGAMLDSDFGVVIGSNALLRRVCRRFGIRILPLAAA--------PLDPRRVPAGT---LYEAVGGWAEIRAFLFGVE 570          

BLAST of mRNA_Ecto-sp13_S_contig8330.19693.1 vs. uniprot
Match: A0A8J5XNG5_DIALT (Thiamine phosphate synthase n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XNG5_DIALT)

HSP 1 Score: 237 bits (604), Expect = 1.930e-62
Identity = 191/566 (33.75%), Postives = 271/566 (47.88%), Query Frame = 2

Query:  209 LPGSLWQDCQLEAMRSLHHPWTLGLATGKTSLASFKNYVAQDAYFLKAFAKAYAYALAKAEDEEDIRAFHSLIGSVLEELGLHASYSAKWGIDVANTPPPVKATAEYVDFLMAVASDPTSSVAQVLAAMVPCMRLYAFLGQELRYAFPNWAESAYAEWIETYSSEDFEDAAGLVESLLDKNSAGADYPSLFALYRKAMELEFAFFDAQLDGGTEAAMLEQSVGAEAGGQQGTSVQPGGKDAGDRGRFHGVHERLALLCVDFDDTLTEGDTTSLLVETAKAQRETPEDRVDLGREWVLLTRTFLSKWSEAIEDSLSTKPSTTACTTNGSGAVDREGLELMLRKLEVVDLDSVSRVSDSKVLRGLTRDEVHSSLAGPLRSSWKVRPGMARCMS--RALVSPFQTHVVSINWSQDVISRVLSETLLESHSSLSSTTGIDARGRVWAGETASPRLEGGCPASVSSNDLVFDEHGVS---TGEI--AVKIPGSFGKHQRFVDLAAMARESVASSESLPRLMTVYVGDSVTDLLAMLDADVGIVVGNSGSFEKVARAFGIDIRPLASVYEAM 1885
            L   LW      A RSLH P+ L LA G    ASF+ YVAQDA+FL+AFA+AYA AL++ E +  I    SLI  V++EL LH  Y+AKW +D+A   P   AT+ Y +FL+A+A    + +A VLAAM PCMRLYAFLGQ L  AFP+ A  AYA+W+ TYSS++F   AG +E+LLD+ + G    +  A Y +AM+LE  FF +Q        +L  +                              +RL L  VDFD+T T+  T  LL + A       ++R     EW  L+ TF  +++ AIE  +       A     +  V+      M+ +L   +  +V      + LRG+   E   + AG  R   K+R G AR     RA  +  +  V+S+NW+  +    L   L             DA   V AG T     E       +   +  D H  +   TGE+  A  +  S  K   F  L A    + A       L+T+Y GDS+TDL  +L A +GIV+G+S S      + G+ ++PL +   A+
Sbjct:    5 LSSKLWASALPLATRSLHSPFVLRLAAGTLPRASFQGYVAQDAFFLRAFARAYALALSRCESDGAIGELSSLIAGVVDELALHHGYAAKWDLDLAGVRP-TAATSAYTEFLLALARS-DAPIAVVLAAMTPCMRLYAFLGQRLCAAFPD-ASHAYADWLRTYSSDEFGALAGRIEALLDECADGVPLDAARAAYTRAMQLELDFFASQPGVAPVDGLLAPA------------------------------DRL-LFAVDFDETATDAQTLPLLAQLAAEHAGAADERAARVAEWRTLSETFAREYTMAIERIVPPXXXXXAPPAGEAAVVE------MVERLSAFERRAVEAAERGRALRGVP--EAALAKAGAERV--KLRDGFARLAYALRAAGACERAEVISVNWAAQLARAALRARL-------------DAARPVGAGATVG--AEELVRLHCNEMPVAIDAHDGAPRTTGELRAAAGLHTSADKGAAFGQLCAEYARAHAGGAG--ELLTLYAGDSITDLQPLLAARIGIVLGHSSSLRAALSSLGVRVQPLVAARRAL 509