mRNA_Ecto-sp13_S_contig8122.19465.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8122.19465.1
Unique NamemRNA_Ecto-sp13_S_contig8122.19465.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Match: A0A6H5L6S1_9PHAE (SGF29 C-terminal domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5L6S1_9PHAE)

HSP 1 Score: 250 bits (639), Expect = 5.230e-73
Identity = 170/174 (97.70%), Postives = 171/174 (98.28%), Query Frame = 2 
Query:    2 VPKPAPGAERRIEVKFNDDADDTGRTPGRPLPARYVVPLPDGFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE 523
            VPKPAPGAERRIEVKFNDDADDTGRTPGR LPARYVVPLP+GFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX  PPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE
Sbjct:  186 VPKPAPGAERRIEVKFNDDADDTGRTPGRSLPARYVVPLPEGFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX--PPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE 357
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BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Match: D7FGV9_ECTSI (SGF29 C-terminal domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FGV9_ECTSI)

HSP 1 Score: 247 bits (631), Expect = 8.870e-69
Identity = 170/178 (95.51%), Postives = 172/178 (96.63%), Query Frame = 2 
Query:    2 VPKPAPGAERRIEVKFNDDADDTGRTPGRPLPARYVVPLPDGFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX----PPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE 523
            VPKPAPGAERRIEVKFNDDADDTGRTPGRPLPARYVVPLP+GFEEDDSK XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX    PPYA+MIRTALMKLPGRQGNL TVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE
Sbjct:  458 VPKPAPGAERRIEVKFNDDADDTGRTPGRPLPARYVVPLPEGFEEDDSKPXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXEPPYAEMIRTALMKLPGRQGNLATVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE 635
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BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Match: A0A7S2D9R3_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2D9R3_9STRA)

HSP 1 Score: 86.7 bits (213), Expect = 1.830e-14
Identity = 55/164 (33.54%), Postives = 75/164 (45.73%), Query Frame = 2 
Query:   35 IEVKFNDDADDTGRTPGRPLPARYVVPLPDGFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHP-----MFCHAGTPERNVYTL 511
            I V+F DD D+TGRTP R + ARYV+   D    DDS                                               Y+DMIR AL KLP +QGN+  +C+ IEE + + LNW+ ES +R+TP WK+S+R+ L          FC      +N++TL
Sbjct:  235 IYVQFEDDEDETGRTPHRRVIARYVILDQDQTGGDDSSVNDLEGNEWGEGDTKTVT----------------------------YSDMIRQALTKLPSQQGNIKDICSMIEEDYSDVLNWKIESDMRKTPVWKSSIRKILVQSQNTNTSRFCTLDE-NKNIFTL 369
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BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Match: A0A8J2SEZ6_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A8J2SEZ6_9STRA)

HSP 1 Score: 80.9 bits (198), Expect = 4.310e-12
Identity = 87/185 (47.03%), Postives = 107/185 (57.84%), Query Frame = 2 
Query:    2 VPKPAPGAE--RRIEVKFNDDADDTGRTPGRPLPARYVVPLPDGFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPP-------------YADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHPMFCH-AGTPERNVYT 508
             PK  PG    R + +KF DD DD+GRTP R +  RYV+      E   +K XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX             P             Y+DMI  AL KLP   G+   +C  IE+ F E+LNW+ ES LR+TP WK+SVR+ L  +  F H     ++N++T
Sbjct:  458 APKRGPGGSPPREVVLKFEDDEDDSGRTPHRRVSLRYVL-----RERPQAKAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQGQAWADKASGGAPADADVAARPGGATYSDMIAHALKKLPDEHGDFKDICKVIEQDFFEQLNWKLESDLRKTPVWKSSVRKILYSNARFRHNLPAADKNIFT 637
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BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Match: A0A4D9CLS8_9STRA (SGF29 C-terminal domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CLS8_9STRA)

HSP 1 Score: 63.9 bits (154), Expect = 6.450e-7
Identity = 53/175 (30.29%), Postives = 69/175 (39.43%), Query Frame = 2 
Query:    2 VPKPAPGAERRIEV--KFNDDADDTGRTPGRPLPARYVVPLPD--GFEEDDSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNWRSESSLRRTPAWKASVRRTLAHHP---MFCHAGTPERNVY 505
            VPK A G    +EV  KF DD D+TGRTP R +PAR+V+ L     FE  +                                                Y  MI  AL KLP  +G L  +   IE  F + L W+ E   +R P WK+ VR+ L +     MF +AG    + Y
Sbjct:  325 VPKRAQGGLGPLEVMVKFEDDEDETGRTPHRRVPARHVLKLSQVGRFEAANGMGRDGEDIGDGGGRKGLRD----------------------------YKIMISHALQKLPNSKGTLKEIFKIIENDFRDELQWKMEGDGKRGPIWKSQVRKLLVNPRSGFMFINAGGERGSQY 471
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8122.19465.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 5
Match NameE-valueIdentityDescription
A0A6H5L6S1_9PHAE5.230e-7397.70SGF29 C-terminal domain-containing protein n=1 Tax... [more]
D7FGV9_ECTSI8.870e-6995.51SGF29 C-terminal domain-containing protein n=1 Tax... [more]
A0A7S2D9R3_9STRA1.830e-1433.54Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A8J2SEZ6_9STRA4.310e-1247.03Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A4D9CLS8_9STRA6.450e-730.29SGF29 C-terminal domain-containing protein n=2 Tax... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8122contigEcto-sp13_S_contig8122:23..4035 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score173.7
Seed ortholog evalue4e-41
Seed eggNOG ortholog2880.D7FGV9
Model size2200
KEGG koko:K03246,ko:K11364
KEGG Pathwayko03013,map03013
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0006325,GO:0006464,GO:0006473,GO:0006475,GO:0006807,GO:0006950,GO:0006970,GO:0006996,GO:0008150,GO:0008152,GO:0009628,GO:0009651,GO:0009987,GO:0016043,GO:0016569,GO:0016570,GO:0016573,GO:0018193,GO:0018205,GO:0018393,GO:0018394,GO:0019538,GO:0036211,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043543,GO:0043966,GO:0043967,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044464,GO:0050896,GO:0051276,GO:0071704,GO:0071840,GO:1901564
Exons4
EggNOG free text desc.methylated histone binding
EggNOG OGsKOG3038@1,KOG3038@2759
Cds size282
COG Functional cat.ADK
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03012,ko03021,ko03036
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8122.19465.1prot_Ecto-sp13_S_contig8122.19465.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8122 267..2334 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464085.0232515-UTR-Ecto-sp13_S_contig8122:22..2661681464085.0232515-UTR-Ecto-sp13_S_contig8122:22..266Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8122 23..266 +
1681464085.0694833-UTR-Ecto-sp13_S_contig8122:2334..30581681464085.0694833-UTR-Ecto-sp13_S_contig8122:2334..3058Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8122 2335..3058 +
1681464085.0823674-UTR-Ecto-sp13_S_contig8122:3085..40351681464085.0823674-UTR-Ecto-sp13_S_contig8122:3085..4035Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8122 3086..4035 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464085.0355227-CDS-Ecto-sp13_S_contig8122:266..2701681464085.0355227-CDS-Ecto-sp13_S_contig8122:266..270Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8122 267..270 +
1681464085.0450504-CDS-Ecto-sp13_S_contig8122:1452..15711681464085.0450504-CDS-Ecto-sp13_S_contig8122:1452..1571Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8122 1453..1571 +
1681464085.0580728-CDS-Ecto-sp13_S_contig8122:2175..23341681464085.0580728-CDS-Ecto-sp13_S_contig8122:2175..2334Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8122 2176..2334 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8122.19465.1

>prot_Ecto-sp13_S_contig8122.19465.1 ID=prot_Ecto-sp13_S_contig8122.19465.1|Name=mRNA_Ecto-sp13_S_contig8122.19465.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=94bp
MGGDEDEDDDDEPPYADMIRTALMKLPGRQGNLPTVCAYIEEHFHERLNW
RSESSLRRTPAWKASVRRTLAHHPMFCHAGTPERNVYTLVQVE*
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mRNA from alignment at Ecto-sp13_S_contig8122:23..4035+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8122.19465.1 ID=mRNA_Ecto-sp13_S_contig8122.19465.1|Name=mRNA_Ecto-sp13_S_contig8122.19465.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=4013bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8122:23..4035+ (Ectocarpus species13 EcNAP12_S_4_19m)
GGTCCCGAAGCCTGCCCCCGGTGCGGAGAGGCGCATCGAGGTCAAGTTCA
ACGACGATGCTGACGACACCGGGCGCACGCCCGGACGGCCTCTGCCAGCT
CGATACGTGGTGCCCCTCCCAGACGGCTTTGAAGAGGACGACAGCAAGCC
TGCCCAAGACGATGAGCCGCCACAAGCGGGGGGCGGCTGCGCTGGTGGCC
GTGGCGGCGTGGATGGTGCCGTAGAGGATGACGGCGACGATCCAATGGGT
GGGTAGTGGTGGGGTGTACACTAAATACCTCTACGTTTGCTGTAGCTGAA
CGTGTAGCAGCAGACGATGCTCGGTTGTGACGAAACCATTGTTGGACAGA
CAGCGCTTAGCTGTAATAACGTATACCCCAGGAGGATAAGACGCAACGAT
GGCCCAGGCAGAAAACACGAAGGCCGCCCGCACAAGATCCAGTCGAGCCA
CCCGATCGATACTGGCGGCTGTGCGGCCTGACACTTTGACTCCGTCAGCC
ATGTTGCCGCCCTCCGATGCACCTGGCGCAAGGCATGGGACCCCCTTTGC
CTGCTCAGCGTGGTGGGTTCGAGTCTGCACGTTTAGTCTGTCTTTTGGTC
CGGAACCCTGACGCGTGCTTCGGATCTTTTTCAGTAGAGGTCCCTGTTGA
GTGCCGGTGGAATTTTTGCGCACGCCCCGGCACTAAACAGGGACCTCTAC
TGTATCTCTCGCTCACGCCTTGAGCCTCGAGGCTGACGTGTTGAGTATTT
CTTGGCTTGGATGATTCTATGGGAAGGAACGTATCCGCCGAAAAAAATGA
TATGGTTTCGGTGTTTGCTATGCTGATGGGTCGGGGTTTGAAAGTGTTGC
CTCGATCGCATTCACCTGAGGCTCTATCGAGCACCCAATCAAAATGAGTA
GCTTGTCCCAACAGGAATCGTGCCCACTGTGTCATGTCGCGACCTCGGGA
TACAGACACCACGAACACACCGAACACACCGTTAAACCAATGTGATGCCG
CTTACAAGCCCCCCGTGACATATCTGGTTTGTCACAGCTAACGCAGGATG
GTGACCAGCCTCTGGCTTATCCTCGTAAAACATAGTCGGCGCCAGAGAAA
ACCCGGCACATCGCTCCGTCCCGGCATTTTGCAGAGACGCGAGGTCAATG
GTTTAACGACTGTGGCAAGCGTTTGCATGAGCACCCGCTGACAGGATAGA
TGGGCTGGGGAAAACATTTGAGCCACCTTTGGCAAAAGCAGGAAGGAACG
ATTGTTGTCCATCGGTGATCGTTCCTGTTGAACAATGCTCCGTCCTCTCG
AGGACGCGTGTCCGATTGTCTAACCGCTAAGGCACGCAAAACGTGAGAGT
ACCCCGACCCCCCCCCCCCATGCACCGCCATTGCGTGCTCCGCCTAGGAC
TGAACCGCGCCGTGTTTTTGTATGTTTCAGGGGGCGACGAAGACGAAGAC
GACGATGACGAGCCGCCGTACGCCGACATGATCCGCACCGCCCTCATGAA
GCTCCCCGGCCGTCAGGGCAACCTGCCAACAGTGTGCGCCTACATCGAGG
TAAGTGGTAAAGAACCTCACCAGATCTCACCCAGAGCAACTGAACCTGGA
CACTGCTGAAGCGTGTCCCAAGCCCCAGATGGTGACGGTAAGGTATGTTC
CCCCCGCCGATATTCTCGTGACACTGCGTGCGTGCGTCTTACATGGGAGG
GTTCCGTCTTTTTTGTTTTTTCGGAGGGTTTCTTGGGTAGGAGGGCGACG
GGAGCCGCTGGCGGGAAACCCGTGCCGTATGTTGACAAGCGTGGTGTGAA
ATGCGGCCATGCTTCCCCTCGATAAGACCCAACCGAAAGCATGCATATGC
ATGGGTATGAGGACGCAGCAGTGTCCGTCGACCGATGTGCATTTGTGTCA
TGTCTGGTTGTTTCTGTTGGTAACGTCCATGTCCAATCACGAAGATGTGA
ACGATGGGGAGGGCAACAATCGTGGGGATTCATGTAGCCCGGCCATCTTC
AGGGCGATAGCAGTGACACCAACCTAGTGCCGCCGGTTGTTCCTTGTTCT
GATGATCCGTCAGCGCTTGGTGATGATTTCCCCAACCCTGCCCCTGATGG
CGTTATGATGGCACGACCACAACCCTATTCCCCGCCGTCGCGTTTGTCGT
CAGGAACACTTCCACGAGCGGTTGAATTGGAGGAGCGAGTCGAGCCTCCG
TCGTACTCCCGCATGGAAGGCGTCCGTCCGCCGCACGCTGGCGCACCACC
CCATGTTCTGCCACGCGGGGACACCAGAGCGGAACGTGTACACGCTCGTA
CAAGTAGAGTGAATGGGCTATACATGTATACCGTAGGTCGCACAAGATAA
GCGCACCCCATGGAAAGAATTTCAGGCTCGATCAACTGCACGAAAGGAGA
GGATGTACACGTGGGATGATTTACGTTTTCGGAAAGCTCTTGACGAGACG
GTTTCCACTGCCAACGGCACCGTGTAACTGTTTTTGCGGCAGAGAAATCC
ATCGGGCTTTGCGTTGTGTCTACCGTGGCTTACGGTATGGAGGGAGGGAA
GGCGGGTGCATCTTCATTTAGTCATTTAGTCTGTTGTTCGAAATCTCATC
CCGGGATCGTGGCGTGGTTTGTTTTGAATGGATGTTCGATGTTTCTTGTT
AGTGGCTAGGGAGCTAGCTGACTGGGCTGCAAAATGAGCCCGGTTCAATT
TCGCGACCCCTAATATTCATTTTTTCAGGAGGGCATTCGGTCGCTATTCA
GGGCAGCTCGCTGTTGCCGATATGCAGTAGCGCCACGCAATTGCTGGTCG
CTATATGTAAATGGTAGGGGTTGTCGCTGAGCAGGGGCGCGATATACCGG
GACGGCGGCCGTTGTGTGTTTTTACGACGAAGGATTTGGCGGTATGGCGT
GACCGATCCCATTATGTATCAGGATCGGAATGCGTCGTGTCATTCGGGTT
GGGTGTGCCTGGCTTTCACGCAGTTGGGTGATGCAGTCGGGGTTGGTTTG
GTTCGCCTGTGTTGGTCTTTTGTTCGATGTTGTAATGCCACGATCGATCA
GGTTTCAGTCGAGGCTACGCATCGATCGCTGCTGTTTTCGCCTTCTTTTT
TTTTTTGCCACTTTTTTTTCTTGTCTGCTCGAGGCATGTAGTGTTGTCCC
TCTATCCGGTTGTTGCGGCAAGAGGAGTTCGCATAGACATCCAAGTATAT
TTCTGTGTATCGCAGCGCCCACTGGTGACACGAAAATTACTGGAGGTTAG
AATTGGGAGGCTACCGTATCGTTAGTGAGCGCTGTACATCTGTTGCCAGA
GGCGAGGGAGAGATGGGCTGTTGTTGGATGGATGAACCGCTTCTTAACAT
TCGTAACTGCCGAGGCGTGTTGGTTCTTTTTGGGGGAGGGGTTCACAGAC
AGTGTTGTGAGGTTCGAAGGGGTATCAGGCTACACCTTCCCGCGATGGAA
TGGAACCATGATTTCGGTTCGGGAAATTGCAGCCTGGTACTCCTATAACG
TCGTGTTTGTAACTTCGGATCGTATCGCCGATTGAGTCACGACAGAAGCT
ATCCTGAGTACAAGCATCTCTCGGGGGCTTGATAACCCCAGTAATTTAAT
AGGCATGGCGATGTCTACATAGACGGTGTGTACGTGTGAAGTCGGTCGGC
TTGACTGCGAAGTGTTGGGTCGGTTGGGGCAGGGCAACGACTGTAGCAGC
AGCAGCAGCATGTACAGGGTCGGGTTGTATGTGTGCGTCTCGTGGACAAA
TCGCCTGCCTGCCATCTGCGTGCGGACGTACACCCGCGACTGCGCATTTT
GGCTGCTAGCTTAAACACGGGGGATGATGGTGTGTTTTTTGTTTTTTCTG
CTGAGCACTCGTTCCTGGTCGCATTTATGGGTCTGCGACACAACGGACGG
TCCCTGTTCTTTTTGCGGTACACAAGGGGACAAGATGAAGTCGTGCGGTG
GTAGGTGTCATGCTTGGTAGGTGGAGGTAGAGGTGGCAGAGCCACATGTC
CGTATTGTCCAAC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8122:23..4035+

>mRNA_Ecto-sp13_S_contig8122.19465.1 ID=mRNA_Ecto-sp13_S_contig8122.19465.1|Name=mRNA_Ecto-sp13_S_contig8122.19465.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=282bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8122:23..4035+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGGGGGCGACGAAGACGAAGACGACGATGACGAGCCGCCGTACGCCGA
CATGATCCGCACCGCCCTCATGAAGCTCCCCGGCCGTCAGGGCAACCTGC
CAACAGTGTGCGCCTACATCGAGGAACACTTCCACGAGCGGTTGAATTGG
AGGAGCGAGTCGAGCCTCCGTCGTACTCCCGCATGGAAGGCGTCCGTCCG
CCGCACGCTGGCGCACCACCCCATGTTCTGCCACGCGGGGACACCAGAGC
GGAACGTGTACACGCTCGTACAAGTAGAGTGA
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