mRNA_Ecto-sp13_S_contig8.19304.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig8.19304.1
Unique NamemRNA_Ecto-sp13_S_contig8.19304.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A6H5KYJ8_9PHAE (Uncharacterized protein (Fragment) n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KYJ8_9PHAE)

HSP 1 Score: 274 bits (701), Expect = 2.790e-87
Identity = 157/174 (90.23%), Postives = 160/174 (91.95%), Query Frame = 2 
Query:   74 MAVQQQQRRRHQPGCGQSPRQSDRSATAGAAAVVMAALALCGGLVEVSEAFLSPATGRLPLAAQTTSRATVAPVAASGSSSAAAGRRAGGICMSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 595
            MAVQQQQR RH+PGCGQSPRQS+RS  A      +A LALCGGLVEVSEAFLSP TGRLP AAQTTSRATVAPVAA GSSS AAGRRAGGICMSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE
Sbjct:    1 MAVQQQQRGRHRPGCGQSPRQSNRSGAAXXXXXXVA-LALCGGLVEVSEAFLSPVTGRLPFAAQTTSRATVAPVAARGSSSVAAGRRAGGICMSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 173
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: D7FXE2_ECTSI (Dihydrolipoamide acetyltransferase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FXE2_ECTSI)

HSP 1 Score: 153 bits (386), Expect = 3.760e-40
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 2 
Query:  350 MSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 595
            MSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE
Sbjct:    1 MSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 82
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A7S1FY38_9STRA (Hypothetical protein (Fragment) n=1 Tax=Corethron hystrix TaxID=216773 RepID=A0A7S1FY38_9STRA)

HSP 1 Score: 115 bits (287), Expect = 1.630e-27
Identity = 58/85 (68.24%), Postives = 71/85 (83.53%), Query Frame = 2 
Query:  371 EMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVERYVRLSREAA 625
            E++MPALSSTM EG IV WLK+VG+K++ GDP+MVVESDKADMDVE+FE+GYLAA+L EEGD A VGAAV +IVE    ++  AA
Sbjct:   65 ELFMPALSSTMTEGKIVTWLKDVGEKVQAGDPIMVVESDKADMDVEAFEDGYLAAILVEEGDMANVGAAVGVIVENEGDVAAVAA 149
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A7S3ZKI1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S3ZKI1_9STRA)

HSP 1 Score: 108 bits (270), Expect = 1.200e-25
Identity = 61/106 (57.55%), Postives = 75/106 (70.75%), Query Frame = 2 
Query:  308 SSSAAAGRRAGGICMSAGKTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVERYVRLSREAA 625
            S SAAA   A      A +++ + MPALSSTM  GTIV WLK VGDK+E GDPVMVVESDKADM+VESF+EGY+A++L +EG  A VGA VAL+      ++  AA
Sbjct:    1 SRSAAAPAAAAAPAGDAPESVSVTMPALSSTMTSGTIVSWLKNVGDKVEAGDPVMVVESDKADMEVESFDEGYVASILVDEGSEADVGAVVALLAPDEASIAEVAA 106
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A7S0N9Z1_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0N9Z1_9CRYP)

HSP 1 Score: 105 bits (263), Expect = 8.350e-24
Identity = 56/89 (62.92%), Postives = 68/89 (76.40%), Query Frame = 2 
Query:  371 EMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVERYVRLSREAAMNRG 637
            ++ MPALSSTM+EG IVQW K VGD++E GD VMVVESDKADMDVE+FE GYLA +L  EG +  VG+AVALIV+    L + AA+  G
Sbjct:   79 DVTMPALSSTMKEGKIVQWTKSVGDRVEAGDIVMVVESDKADMDVEAFESGYLAKILVPEGSAGAVGSAVALIVQDKADLDKVAAVAAG 167
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A836C7H2_9STRA (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C7H2_9STRA)

HSP 1 Score: 112 bits (279), Expect = 1.510e-23
Identity = 58/77 (75.32%), Postives = 68/77 (88.31%), Query Frame = 2 
Query:  365 TIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 595
            T E+ MPALSSTM EG IVQWLKEVGD+++VGD VMVVESDKADMDVESFE G+LAA+LT EG++A VGAAVA++ E
Sbjct:   10 TKEIIMPALSSTMTEGKIVQWLKEVGDEVQVGDAVMVVESDKADMDVESFEAGFLAAILTPEGEAAPVGAAVAVLAE 86
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A7J7IHU4_9RHOD (Lipoyl-binding domain-containing protein n=1 Tax=Cyanidiococcus yangmingshanensis TaxID=2690220 RepID=A0A7J7IHU4_9RHOD)

HSP 1 Score: 107 bits (266), Expect = 2.010e-23
Identity = 58/82 (70.73%), Postives = 68/82 (82.93%), Query Frame = 2 
Query:  371 EMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALI---VERYVR 607
            E+++PALSSTM EG IVQWLK+VGD+IE GD V+VVESDKADMDVE+FEEGYLA +L +EG+SA VGA V LI   VE Y R
Sbjct:  156 EVFLPALSSTMTEGKIVQWLKQVGDRIEKGDIVLVVESDKADMDVEAFEEGYLAQILVKEGESAPVGATVGLIAKTVEGYRR 237
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: B8C489_THAPS (Lipoyl-binding domain-containing protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C489_THAPS)

HSP 1 Score: 102 bits (255), Expect = 3.050e-23
Identity = 54/95 (56.84%), Postives = 72/95 (75.79%), Query Frame = 2 
Query:  365 TIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVERYVRLSREAAMNRGHGGD 649
            +I++ MPALSSTM+EG +V WLK+ GD+IE G+ +MVVESDKADMDVE+FE+GYLA +LT EG++A+VGA VAL+         + A+    GGD
Sbjct:    4 SIKITMPALSSTMKEGRVVSWLKQEGDEIEAGEAIMVVESDKADMDVEAFEDGYLAKILTGEGETAEVGAVVALVATS----EEDIAVVAAGGGD 94
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A3D1PBD9_9CYAN (Dihydrolipoyllysine-residue acetyltransferase (Fragment) n=1 Tax=Cyanobacteria bacterium UBA11049 TaxID=2055787 RepID=A0A3D1PBD9_9CYAN)

HSP 1 Score: 100 bits (250), Expect = 6.310e-23
Identity = 50/81 (61.73%), Postives = 63/81 (77.78%), Query Frame = 2 
Query:  371 EMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVERYVRLS 613
            E++MPALSSTM EG IV W+K  GDK+E G+ V+VVESDKADMDVESF EGY+A +L   G+SA VGAA+AL+ E    ++
Sbjct:    4 EVFMPALSSTMTEGKIVSWVKSPGDKVEKGETVVVVESDKADMDVESFYEGYIATILVNAGESASVGAAIALVAETEAEIA 84
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BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Match: A0A7S3E7J0_9RHOD (Hypothetical protein n=1 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3E7J0_9RHOD)

HSP 1 Score: 109 bits (273), Expect = 6.720e-23
Identity = 56/78 (71.79%), Postives = 68/78 (87.18%), Query Frame = 2 
Query:  362 KTIEMYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEGYLAAVLTEEGDSAKVGAAVALIVE 595
            KT +++MPALSSTM EG IVQWLK+ GD ++VG+ +MVVESDKADMDVESFE+GYLA +LTEEG+SA VGA VAL+ E
Sbjct:   58 KTKDVFMPALSSTMTEGRIVQWLKQPGDFVDVGEIIMVVESDKADMDVESFEKGYLAQILTEEGESAGVGATVALLAE 135
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig8.19304.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KYJ8_9PHAE2.790e-8790.23Uncharacterized protein (Fragment) n=1 Tax=Ectocar... [more]
D7FXE2_ECTSI3.760e-40100.00Dihydrolipoamide acetyltransferase n=1 Tax=Ectocar... [more]
A0A7S1FY38_9STRA1.630e-2768.24Hypothetical protein (Fragment) n=1 Tax=Corethron ... [more]
A0A7S3ZKI1_9STRA1.200e-2557.55Hypothetical protein (Fragment) n=1 Tax=Pelagomona... [more]
A0A7S0N9Z1_9CRYP8.350e-2462.92Hypothetical protein (Fragment) n=1 Tax=Cryptomona... [more]
A0A836C7H2_9STRA1.510e-2375.32Dihydrolipoamide acetyltransferase component of py... [more]
A0A7J7IHU4_9RHOD2.010e-2370.73Lipoyl-binding domain-containing protein n=1 Tax=C... [more]
B8C489_THAPS3.050e-2356.84Lipoyl-binding domain-containing protein n=1 Tax=T... [more]
A0A3D1PBD9_9CYAN6.310e-2361.73Dihydrolipoyllysine-residue acetyltransferase (Fra... [more]
A0A7S3E7J0_9RHOD6.720e-2371.79Hypothetical protein n=1 Tax=Rhodosorus marinus Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig8contigEcto-sp13_S_contig8:1632..3770 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score155.2
Seed ortholog evalue3.2e-35
Seed eggNOG ortholog2880.D7FXE2
Model size1043
KEGG rclassRC00004,RC02742,RC02857
KEGG koko:K00627
KEGG ReactionR00209,R02569
KEGG Pathwayko00010,ko00020,ko00620,ko01100,ko01110,ko01120,ko01130,ko01200,map00010,map00020,map00620,map01100,map01110,map01120,map01130,map01200
KEGG ModuleM00307
Hectar predicted targeting categoryother localisation
Exons3
EggNOG free text desc.dihydrolipoyllysine-residue acetyltransferase activity
EggNOG OGsCOG0508@1,KOG0557@2759
EC2.3.1.12
Cds size600
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464071.1025457-UTR-Ecto-sp13_S_contig8:1631..20011681464071.1025457-UTR-Ecto-sp13_S_contig8:1631..2001Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8 1632..2001 -
1681464071.154868-UTR-Ecto-sp13_S_contig8:3697..37701681464071.154868-UTR-Ecto-sp13_S_contig8:3697..3770Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig8 3698..3770 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681464071.1207988-CDS-Ecto-sp13_S_contig8:2001..22201681464071.1207988-CDS-Ecto-sp13_S_contig8:2001..2220Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8 2002..2220 -
1681464071.135969-CDS-Ecto-sp13_S_contig8:2955..30511681464071.135969-CDS-Ecto-sp13_S_contig8:2955..3051Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8 2956..3051 -
1681464071.145577-CDS-Ecto-sp13_S_contig8:3412..36971681464071.145577-CDS-Ecto-sp13_S_contig8:3412..3697Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig8 3413..3697 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig8.19304.1prot_Ecto-sp13_S_contig8.19304.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig8 2002..3697 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig8.19304.1

>prot_Ecto-sp13_S_contig8.19304.1 ID=prot_Ecto-sp13_S_contig8.19304.1|Name=mRNA_Ecto-sp13_S_contig8.19304.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=200bp
MAVQQQQRRRHQPGCGQSPRQSDRSATAGAAAVVMAALALCGGLVEVSEA
FLSPATGRLPLAAQTTSRATVAPVAASGSSSAAAGRRAGGICMSAGKTIE
MYMPALSSTMEEGTIVQWLKEVGDKIEVGDPVMVVESDKADMDVESFEEG
YLAAVLTEEGDSAKVGAAVALIVERYVRLSREAAMNRGHGGDISVELVV*
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mRNA from alignment at Ecto-sp13_S_contig8:1632..3770-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig8.19304.1 ID=mRNA_Ecto-sp13_S_contig8.19304.1|Name=mRNA_Ecto-sp13_S_contig8.19304.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=2139bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8:1632..3770- (Ectocarpus species13 EcNAP12_S_4_19m)
CAGCGCGGCAGCAGCAGCAAAACCGGCGCTGGAACGGTTGTGTTCGCACA
GCAGCAGCATTAGCGAGGCGACCATGGCTGTTCAGCAGCAGCAGCGGCGG
CGGCATCAGCCGGGGTGCGGTCAATCGCCCCGGCAATCAGACCGGTCAGC
GACGGCGGGCGCGGCGGCCGTAGTCATGGCGGCGCTCGCGCTGTGCGGGG
GGTTGGTGGAGGTTTCCGAGGCGTTTCTGTCGCCCGCGACGGGGCGGTTG
CCGTTAGCGGCGCAGACAACCTCGAGGGCGACCGTAGCGCCGGTGGCGGC
AAGTGGGTCGTCTTCTGCTGCCGCAGGCCGAAGGGCGGGAGGGATTTGCA
TGTCGGCGGTGAGGAGATTTTGGGGAATAGTGAGGCCGGGGAAGGGGGGG
GGGGCATGCAATCGACCGCTACAGAGCTGTCCATGTCTGCGTCGGCATCC
TCGTGGTTATCACGAGGAGCTTTGATGATCAGTCCTGAAAGTGTGCGGCT
GGCTACCCGCAGCAGTTTTATTCTTGGTTTGTTTTGTCGTTTGTTCTGTG
TGTGCTTTCGAGAATCGCACCGTTTGCGTGCCGTCGTCTCTTCACTGCTC
GCCTCGAAAAGTACCGTCGCAGCAGCAGTTGGTGAATGTTGTGCGTTGCC
CACGTCAACCCCACCCACAACAACCACCCGTGCCTATTCCTGGAACGCCT
TCCTCCTGGCATTGTCCAGGGCAAGACCATTGAGATGTACATGCCGGCAC
TCTCGTCGACGATGGAGGAAGGGACGATCGTGCAGTGGCTCAAGGAAGTC
GGCGACAAGATCGAGGTGACTGACTGACGGACTCACCGCGCGGTTGCGGC
GTCCACTCCGCAAGGACTGGATTTGGTGTTGTCCTCTTACGCGAGGAGCT
ACTGTGCTGCTGGTGCATCGGAACAAAGACTAAGGCTGTCGGTTGTGCCA
CTGGTGGTTGCGTTGGTCTACAGTAGGTATTTCCTCGGAGTCAACATCCT
CAGCGTTGCCACCCCTCCCCAGTGTCTACGCTGGGTTTGGTGGGAGAAAG
AACCACTACAGCTCGGCGTGGTATGCGGTAGCTTAGACGTATGCATAGAA
AACGCACCCCGGCGGGATGTATAGTCCTTCTCCCTCAAAAATGCACCACG
CCGGCGCGTCCTCCACGGTATTTACCATGGTAAAAACCACGGGAAGGCAT
GAACGTCCCTTGGTATTTCCCCGCAGCCGCCGCTAGTACCACCGTGACCC
GGAAGTTACGGTGCAGGTACGACGAGCAAACGCAACCAACAACGAACGGT
TCATTTGGCCTTCCCTTCCACCATAGCACTTAGAGATCGCAGATCCTATA
GCAGGTCGTCGGCGACTGTCACAGTGCGGTTAGCGCAGCCCCATGCCGAC
AAACACAACGTGACACGTGCAACAGTGCTACGACCCTCGTCCGCGGCAAA
GTGCCCCGAAGAATCGTCGCCCGCGTCATGTAGTTGGTTACGACGTGGAG
CGTGTTGTCTGCCCCCGCCTCCCCTTCGATAACCACGCGACCGCGCTTAG
GTGGGAGACCCCGTGATGGTGGTGGAGTCGGATAAGGCTGACATGGATGT
GGAGTCCTTCGAGGAGGGGTACCTGGCGGCCGTGCTGACGGAGGAAGGGG
ACAGCGCCAAGGTCGGGGCGGCCGTCGCTCTCATCGTTGAGAGGTATGTA
CGTCTCTCGAGGGAAGCAGCGATGAATCGGGGCCACGGCGGTGATATTTC
GGTTGAGCTGGTGGTGTAGCCGAAGCCCTCGAACGCTATTGCTGTTGGTA
TGTGGATGAGGGAGGTGTAGCGGAGTGTTGCGACGCTAGCCGGCGCAGTG
TTGAGCGCGAGCAGGAACATGACACCAACGGCCACGATCATGCCCGTCCC
CGTGAATGGACCTAGACAGATAGATAGATAGGCCAGCAGTAGGTAGGACC
CGCAGCCGCTTTTGACTGTTGCTGGACAGTTCGTCGACGGCCTCTCGATT
TGCTCGTTGGACATACATGTCATGTTGCGTTTTGGCCGTCCAGCTGAGAT
CCTTGCACCCAAACCTGACAGAATTGTGACTGTTTCGTTCTGCATCCTCC
CGGGCTTCGGATCACGGGTGGTTGGAAGGACCCCGCTGC
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig8:1632..3770-

>mRNA_Ecto-sp13_S_contig8.19304.1 ID=mRNA_Ecto-sp13_S_contig8.19304.1|Name=mRNA_Ecto-sp13_S_contig8.19304.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=600bp|location=Sequence derived from alignment at Ecto-sp13_S_contig8:1632..3770- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGGCTGTTCAGCAGCAGCAGCGGCGGCGGCATCAGCCGGGGTGCGGTCA
ATCGCCCCGGCAATCAGACCGGTCAGCGACGGCGGGCGCGGCGGCCGTAG
TCATGGCGGCGCTCGCGCTGTGCGGGGGGTTGGTGGAGGTTTCCGAGGCG
TTTCTGTCGCCCGCGACGGGGCGGTTGCCGTTAGCGGCGCAGACAACCTC
GAGGGCGACCGTAGCGCCGGTGGCGGCAAGTGGGTCGTCTTCTGCTGCCG
CAGGCCGAAGGGCGGGAGGGATTTGCATGTCGGCGGGCAAGACCATTGAG
ATGTACATGCCGGCACTCTCGTCGACGATGGAGGAAGGGACGATCGTGCA
GTGGCTCAAGGAAGTCGGCGACAAGATCGAGGTGGGAGACCCCGTGATGG
TGGTGGAGTCGGATAAGGCTGACATGGATGTGGAGTCCTTCGAGGAGGGG
TACCTGGCGGCCGTGCTGACGGAGGAAGGGGACAGCGCCAAGGTCGGGGC
GGCCGTCGCTCTCATCGTTGAGAGGTATGTACGTCTCTCGAGGGAAGCAG
CGATGAATCGGGGCCACGGCGGTGATATTTCGGTTGAGCTGGTGGTGTAG
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