mRNA_Ecto-sp13_S_contig66063.17451.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig66063.17451.1
Unique NamemRNA_Ecto-sp13_S_contig66063.17451.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: D7FUR8_ECTSI (Nitrate high affinity transporter n=4 Tax=Ectocarpus TaxID=2879 RepID=D7FUR8_ECTSI)

HSP 1 Score: 150 bits (379), Expect = 6.280e-42
Identity = 73/74 (98.65%), Postives = 73/74 (98.65%), Query Frame = 1 
Query:    1 AIVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            AIVFCVAVGQLFLADDSPKGNYKELE HGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF
Sbjct:  205 AIVFCVAVGQLFLADDSPKGNYKELEAHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 278
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: D7FUS1_ECTSI (Nitrate/nitrite transporter n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FUS1_ECTSI)

HSP 1 Score: 142 bits (357), Expect = 1.030e-38
Identity = 67/74 (90.54%), Postives = 70/74 (94.59%), Query Frame = 1 
Query:    1 AIVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            AIVFCVA+GQ+FLADD PKGNYKELE HGAMTRKSSAVSFKKGY NVNSW+MF QYAACFGVELTVNNVAANYF
Sbjct:  205 AIVFCVAIGQVFLADDCPKGNYKELEAHGAMTRKSSAVSFKKGYSNVNSWIMFAQYAACFGVELTVNNVAANYF 278
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: A0A1Z5K8V7_FISSO (Nitrate/nitrite transporter n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K8V7_FISSO)

HSP 1 Score: 100 bits (250), Expect = 2.410e-23
Identity = 42/73 (57.53%), Postives = 57/73 (78.08%), Query Frame = 1 
Query:    4 IVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            + FC  +G LF  +D+PKGNY E+++HG M   S+AVSF+ G +N N+W++F+QYAACFGVELT+NN AA YF
Sbjct:  215 VTFCTGLGMLFFTEDAPKGNYGEMKKHGTMPEVSAAVSFRAGAVNFNTWILFIQYAACFGVELTMNNAAALYF 287
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: E9KZI3_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured diatom TaxID=137771 RepID=E9KZI3_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 7.690e-23
Identity = 40/62 (64.52%), Postives = 52/62 (83.87%), Query Frame = 1 
Query:   37 LADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            ++DDSPKGNYKEL++HG M   S+A SF++G +N+N+W MF+QYA CFGVELT+NN AA YF
Sbjct:   55 ISDDSPKGNYKELQKHGMMAEVSAAASFRQGALNLNTWFMFIQYACCFGVELTMNNAAALYF 116
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: A4URD1_9STRA (NRT2 (Fragment) n=2 Tax=Ditylum brightwellii TaxID=49249 RepID=A4URD1_9STRA)

HSP 1 Score: 94.4 bits (233), Expect = 3.580e-22
Identity = 40/73 (54.79%), Postives = 54/73 (73.97%), Query Frame = 1 
Query:    4 IVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            + F   +   F++DDSPKGNY +L++HG M   S+A SF+ G MN N+W++F+QYA CFGVELT+NN AA YF
Sbjct:   69 VAFTTGITVYFISDDSPKGNYSDLKKHGNMPEVSAAASFRSGAMNFNTWILFIQYACCFGVELTMNNAAALYF 141
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: A0A7S2E7Y3_TRICV (Hypothetical protein (Fragment) n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2E7Y3_TRICV)

HSP 1 Score: 94.7 bits (234), Expect = 6.220e-22
Identity = 39/64 (60.94%), Postives = 55/64 (85.94%), Query Frame = 1 
Query:   31 LFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            LF++DD+PKGNYKE++++G M   S+A SF+ G++N+N+WL+F+QYA CFGVELT+NN AA YF
Sbjct:  194 LFISDDAPKGNYKEMKKNGTMPDVSAAASFRTGFLNINTWLLFIQYACCFGVELTMNNAAALYF 257
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: E9KZI1_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured diatom TaxID=137771 RepID=E9KZI1_9STRA)

HSP 1 Score: 91.7 bits (226), Expect = 8.330e-22
Identity = 41/73 (56.16%), Postives = 55/73 (75.34%), Query Frame = 1 
Query:    4 IVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            I F   V   F++DD+PKGNYKEL+++G M   S+A SF+ G +N+N+W++FLQY  CFGVELT+NN AA YF
Sbjct:   42 IGFMSGVAVYFISDDAPKGNYKELKKNGLMPEVSAAASFRTGALNLNTWVLFLQYGCCFGVELTMNNAAALYF 114
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: E9KZH6_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured diatom TaxID=137771 RepID=E9KZH6_9STRA)

HSP 1 Score: 91.7 bits (226), Expect = 8.550e-22
Identity = 39/64 (60.94%), Postives = 53/64 (82.81%), Query Frame = 1 
Query:   31 LFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            L ++DD+PKGNYKE++++G M   S+A SF+ G MN N+WL+F+QYA CFGVELT+NN AA+YF
Sbjct:   52 LKISDDAPKGNYKEMKKNGTMPEVSAAASFRSGAMNFNTWLLFIQYACCFGVELTMNNAAASYF 115
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: E9KZG6_9STRA (High affinity nitrate transporter (Fragment) n=1 Tax=uncultured Rhizosolenia TaxID=941242 RepID=E9KZG6_9STRA)

HSP 1 Score: 91.3 bits (225), Expect = 1.210e-21
Identity = 41/73 (56.16%), Postives = 55/73 (75.34%), Query Frame = 1 
Query:    4 IVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            + F   +  L ++DDSPKGNY EL++HG+M   S+A SF++G  N N+WL+F+QYA CFGVELT+NN AA YF
Sbjct:   43 VAFSWGLTILRISDDSPKGNYAELKKHGSMADVSAAASFRQGAFNFNTWLLFIQYACCFGVELTMNNAAALYF 115
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BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Match: A0A6U6IY47_9STRA (Nitrate/nitrite transporter n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A6U6IY47_9STRA)

HSP 1 Score: 96.3 bits (238), Expect = 1.230e-21
Identity = 42/73 (57.53%), Postives = 56/73 (76.71%), Query Frame = 1 
Query:    4 IVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSWLMFLQYAACFGVELTVNNVAANYF 222
            + F   V  LF++DD+PKGNYK+L+ +G M   S+A SF+ G +NVN+WL+F+QYA CFGVELT+NN AA YF
Sbjct:  220 VAFASGVAILFISDDAPKGNYKDLKRNGLMPEVSAAASFRSGALNVNTWLLFIQYACCFGVELTMNNAAALYF 292
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig66063.17451.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FUR8_ECTSI6.280e-4298.65Nitrate high affinity transporter n=4 Tax=Ectocarp... [more]
D7FUS1_ECTSI1.030e-3890.54Nitrate/nitrite transporter n=2 Tax=Ectocarpus Tax... [more]
A0A1Z5K8V7_FISSO2.410e-2357.53Nitrate/nitrite transporter n=2 Tax=Fistulifera so... [more]
E9KZI3_9STRA7.690e-2364.52High affinity nitrate transporter (Fragment) n=1 T... [more]
A4URD1_9STRA3.580e-2254.79NRT2 (Fragment) n=2 Tax=Ditylum brightwellii TaxID... [more]
A0A7S2E7Y3_TRICV6.220e-2260.94Hypothetical protein (Fragment) n=1 Tax=Trieres ch... [more]
E9KZI1_9STRA8.330e-2256.16High affinity nitrate transporter (Fragment) n=1 T... [more]
E9KZH6_9STRA8.550e-2260.94High affinity nitrate transporter (Fragment) n=1 T... [more]
E9KZG6_9STRA1.210e-2156.16High affinity nitrate transporter (Fragment) n=1 T... [more]
A0A6U6IY47_9STRA1.230e-2157.53Nitrate/nitrite transporter n=1 Tax=Odontella auri... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig66063contigEcto-sp13_S_contig66063:2..468 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score150.6
Seed ortholog evalue2.9e-34
Seed eggNOG ortholog2880.D7FUR8
Preferred nameNRT2.2
Model size224
KEGG koko:K02575
KEGG TC2.A.1.8
KEGG Pathwayko00910,map00910
KEGG ModuleM00615
Hectar predicted targeting categorysignal peptide
GOsGO:0000325,GO:0001101,GO:0003674,GO:0005215,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0005886,GO:0006810,GO:0006811,GO:0006820,GO:0007275,GO:0008150,GO:0008509,GO:0009507,GO:0009536,GO:0009628,GO:0009705,GO:0009791,GO:0009987,GO:0010035,GO:0010167,GO:0015075,GO:0015103,GO:0015112,GO:0015267,GO:0015291,GO:0015318,GO:0015513,GO:0015698,GO:0015706,GO:0015707,GO:0016020,GO:0022622,GO:0022803,GO:0022804,GO:0022832,GO:0022836,GO:0022857,GO:0031090,GO:0032501,GO:0032502,GO:0032991,GO:0034220,GO:0042221,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0048364,GO:0048527,GO:0048528,GO:0048731,GO:0048856,GO:0050896,GO:0051179,GO:0051234,GO:0051716,GO:0055085,GO:0070887,GO:0071229,GO:0071241,GO:0071249,GO:0071705,GO:0071944,GO:0080167,GO:0090696,GO:0098588,GO:0098656,GO:0098805,GO:0099402,GO:1901698,GO:1901699,GO:1901700,GO:1901701,GO:1902170,GO:1990351
Exons2
EggNOG free text desc.cellular response to nitrate
EggNOG OGs2QPIC@2759,COG2223@1
Ec32 ortholog descriptionNitrate high affinity transporter; pseudogene
Ec32 orthologEc-19_004160.1
Cds size222
COG Functional cat.P
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463910.0515711-CDS-Ecto-sp13_S_contig66063:2..981681463910.0515711-CDS-Ecto-sp13_S_contig66063:2..98Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig66063 3..98 -
1681463910.0734777-CDS-Ecto-sp13_S_contig66063:342..4681681463910.0734777-CDS-Ecto-sp13_S_contig66063:342..468Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig66063 343..468 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig66063.17451.1prot_Ecto-sp13_S_contig66063.17451.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig66063 3..468 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig66063.17451.1

>prot_Ecto-sp13_S_contig66063.17451.1 ID=prot_Ecto-sp13_S_contig66063.17451.1|Name=mRNA_Ecto-sp13_S_contig66063.17451.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=74bp
AIVFCVAVGQLFLADDSPKGNYKELEEHGAMTRKSSAVSFKKGYMNVNSW
LMFLQYAACFGVELTVNNVAANYF
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mRNA from alignment at Ecto-sp13_S_contig66063:2..468-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig66063.17451.1 ID=mRNA_Ecto-sp13_S_contig66063.17451.1|Name=mRNA_Ecto-sp13_S_contig66063.17451.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=467bp|location=Sequence derived from alignment at Ecto-sp13_S_contig66063:2..468- (Ectocarpus species13 EcNAP12_S_4_19m)
GCCATCGTGTTCTGCGTTGCCGTGGGCCAGCTGTTCCTCGCCGATGACAG
CCCGAAGGGTAACTACAAGGAGCTCGAGGAGCACGGCGCCATGACTCGCA
AGTCCTCCGCCGTTTCCTTCAAGAAGGTGAATGAGACGGGATTGGTCCTG
TGTCTATACCAGAGTTCAGTATGGTCGAGTTAAGCTGATGCATGCTGTAG
TGTGCCCCATCTTGGCGCGATGGTTGGTGGTGCGGTGACTATTCAGCATC
ATGCGGGACGGTCTCTCTTCCATTCTACGCCAGTTGCTGCTGCCATCTAC
CACTCTCACGTTTGCCCGTTCGCTTGCCCTCTTGCCTCCCCTTGCAATCG
CCTCCCCATTCGCTGAGCAGGGGTACATGAACGTGAACTCATGGCTCATG
TTTCTCCAGTACGCCGCCTGCTTCGGCGTCGAGCTGACTGTCAACAACGT
GGCGGCCAACTACTTCT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig66063:2..468-

>mRNA_Ecto-sp13_S_contig66063.17451.1 ID=mRNA_Ecto-sp13_S_contig66063.17451.1|Name=mRNA_Ecto-sp13_S_contig66063.17451.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=222bp|location=Sequence derived from alignment at Ecto-sp13_S_contig66063:2..468- (Ectocarpus species13 EcNAP12_S_4_19m)
GCCATCGTGTTCTGCGTTGCCGTGGGCCAGCTGTTCCTCGCCGATGACAG
CCCGAAGGGTAACTACAAGGAGCTCGAGGAGCACGGCGCCATGACTCGCA
AGTCCTCCGCCGTTTCCTTCAAGAAGGGGTACATGAACGTGAACTCATGG
CTCATGTTTCTCCAGTACGCCGCCTGCTTCGGCGTCGAGCTGACTGTCAA
CAACGTGGCGGCCAACTACTTC
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