mRNA_Ecto-sp13_S_contig561.15943.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig561.15943.1
Unique NamemRNA_Ecto-sp13_S_contig561.15943.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A6H5KTV8_9PHAE (ATPase ASNA1 homolog n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KTV8_9PHAE)

HSP 1 Score: 266 bits (681), Expect = 5.470e-87
Identity = 135/138 (97.83%), Postives = 135/138 (97.83%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGG   ATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV
Sbjct:  129 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGG---ATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 263
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A835ZI69_9STRA (ATPase ASNA1 homolog n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZI69_9STRA)

HSP 1 Score: 230 bits (586), Expect = 1.270e-72
Identity = 114/138 (82.61%), Postives = 126/138 (91.30%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAELMKMVQ MNFSCI+FDTAPTGHTLRLLSFPTIMDKAL+K+M LK+KF+G   QFG MFGAGG   A+ DD+ GKL Q+++IINKVNDQFKDP++TTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV
Sbjct:  135 MSFAELMKMVQVMNFSCIIFDTAPTGHTLRLLSFPTIMDKALTKIMALKDKFAGALGQFGAMFGAGG---ASPDDLMGKLEQMRDIINKVNDQFKDPERTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 269
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: I3T558_LOTJA (ArsA_ATPase domain-containing protein n=2 Tax=NPAAA clade TaxID=2231382 RepID=I3T558_LOTJA)

HSP 1 Score: 197 bits (502), Expect = 4.740e-61
Identity = 96/138 (69.57%), Postives = 115/138 (83.33%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FP++++K L+K+M LKNKF GLF+Q   MFG G      ED + GKL  +K++I +VN QFKDPD TTFVCVCIPEFLSLYETERLVQELTKFEIDTHNI++NQV
Sbjct:   36 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSVLEKGLAKMMSLKNKFGGLFNQMTRMFGMG--DDFGEDAILGKLEGMKDVIEQVNKQFKDPDMTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIIINQV 171
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A1V9Y4X4_9STRA (ATPase ASNA1 homolog n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9Y4X4_9STRA)

HSP 1 Score: 198 bits (504), Expect = 9.470e-61
Identity = 98/138 (71.01%), Postives = 115/138 (83.33%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAELMK VQNM++S IVFDTAPTGHTLRLLSFPT +DKA  K+M LKN+FSG+F Q   +FG    S  +E+ + GKL Q +E+I KVN+QFKD D+TTFVCVCIPEFLSLYETERLVQELTK++ID HN+VVNQV
Sbjct:  123 MSFAELMKQVQNMDYSVIVFDTAPTGHTLRLLSFPTALDKAFGKIMALKNQFSGMFGQVSALFGG---SLPSEEMLVGKLEQTREVIQKVNEQFKDADRTTFVCVCIPEFLSLYETERLVQELTKYDIDVHNVVVNQV 257
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A2G9G4G6_9LAMI (ATPase n=1 Tax=Handroanthus impetiginosus TaxID=429701 RepID=A0A2G9G4G6_9LAMI)

HSP 1 Score: 199 bits (506), Expect = 1.310e-60
Identity = 97/138 (70.29%), Postives = 114/138 (82.61%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FP  ++K L+K+M LKNKF GLFSQ   +FG G      EDD+ GKL  +K++I +VN QFKDPD TTFVCVCIPEFLSLYETERLVQELTKFEIDTHNI++NQV
Sbjct:  121 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPATLEKGLAKMMSLKNKFGGLFSQMTRLFGVG--DDFGEDDILGKLEGMKDVIQQVNKQFKDPDMTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIIINQV 256
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A8M8VB14_SESIN (ATPase GET3 n=1 Tax=Sesamum indicum TaxID=4182 RepID=A0A8M8VB14_SESIN)

HSP 1 Score: 197 bits (502), Expect = 4.810e-60
Identity = 96/138 (69.57%), Postives = 115/138 (83.33%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FPT ++K L+K+M L++KF GLFSQ   +FG G      EDD+ GKL  +K++I +VN QFKDPD TTFVCVCIPEFLSLYETERLVQELTKFEIDTHNI++NQV
Sbjct:  118 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPTTLEKGLAKVMSLRSKFGGLFSQMTRLFGVG--DDFGEDDLLGKLEGMKDVIEQVNKQFKDPDLTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIIINQV 253
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A072TNB3_MEDTR (ATPase 25500968 n=5 Tax=IRL clade TaxID=2233839 RepID=A0A072TNB3_MEDTR)

HSP 1 Score: 197 bits (502), Expect = 5.220e-60
Identity = 97/138 (70.29%), Postives = 115/138 (83.33%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FP++++K L+K+M LKNKF GLFSQ   MFG G      ED + GKL  +K++I +VN QFKDPD TTFVCVCIPEFLSLYETERLVQELTKFEIDTHNI++NQV
Sbjct:  121 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSVLEKGLAKVMSLKNKFGGLFSQMTRMFGMG--DDFGEDAILGKLEGMKDVIEQVNMQFKDPDMTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIIINQV 256
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A5A7QM57_STRAF (ATPase n=3 Tax=Orobanchaceae TaxID=91896 RepID=A0A5A7QM57_STRAF)

HSP 1 Score: 197 bits (502), Expect = 5.220e-60
Identity = 95/138 (68.84%), Postives = 116/138 (84.06%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FP+ ++K L+K+M LKNKF GLFSQ   +FG G      EDD+ G+L  +K++I +VN+QFKDPD TTFVCVCIPEFLSLYETERLVQELT+FEIDTHNI++NQV
Sbjct:  121 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSTLEKGLAKMMTLKNKFGGLFSQMTRLFGVG--DDFGEDDILGRLEGMKDVIEQVNNQFKDPDMTTFVCVCIPEFLSLYETERLVQELTRFEIDTHNIIINQV 256
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: A0A4D8XZB4_SALSN (ATPase arsA n=5 Tax=Salvia TaxID=21880 RepID=A0A4D8XZB4_SALSN)

HSP 1 Score: 197 bits (500), Expect = 1.070e-59
Identity = 96/138 (69.57%), Postives = 114/138 (82.61%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSFAE++K+VQ M++S IVFDTAPTGHTLRLL FP+ ++K LSK+M +KN+F GL SQ   +FGAG      ED + GKL  LKE+I +VN QFKDPD TTF+CVCIPEFLSLYETERLVQELTKFEIDTHNI++NQV
Sbjct:  122 MSFAEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSTLEKGLSKIMTIKNRFGGLLSQMTRLFGAG--EEFNEDAILGKLEGLKEVIEQVNTQFKDPDLTTFICVCIPEFLSLYETERLVQELTKFEIDTHNIIINQV 257
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BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Match: UPI0020097BAB (ATPase GET3A-like n=1 Tax=Salvia hispanica TaxID=49212 RepID=UPI0020097BAB)

HSP 1 Score: 193 bits (491), Expect = 1.170e-59
Identity = 94/138 (68.12%), Postives = 114/138 (82.61%), Query Frame = 1 
Query:    1 MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKNKFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKTTFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQV 414
            MSF E++K+VQ M++S IVFDTAPTGHTLRLL FP+ ++K LSKLM L+NKF G+F+Q   MFG    +   EDD+ GKL  +K++I +VN QFKDPD TTF+CVCIPEFLSLYETERLVQELT+FEIDTHNIV+NQV
Sbjct:   15 MSFGEMLKLVQTMDYSVIVFDTAPTGHTLRLLQFPSTLEKGLSKLMSLRNKFGGMFTQMTRMFGVE--NDFGEDDILGKLEGMKDVIEQVNRQFKDPDLTTFICVCIPEFLSLYETERLVQELTRFEIDTHNIVINQV 150
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig561.15943.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KTV8_9PHAE5.470e-8797.83ATPase ASNA1 homolog n=1 Tax=Ectocarpus sp. CCAP 1... [more]
A0A835ZI69_9STRA1.270e-7282.61ATPase ASNA1 homolog n=1 Tax=Tribonema minus TaxID... [more]
I3T558_LOTJA4.740e-6169.57ArsA_ATPase domain-containing protein n=2 Tax=NPAA... [more]
A0A1V9Y4X4_9STRA9.470e-6171.01ATPase ASNA1 homolog n=1 Tax=Achlya hypogyna TaxID... [more]
A0A2G9G4G6_9LAMI1.310e-6070.29ATPase n=1 Tax=Handroanthus impetiginosus TaxID=42... [more]
A0A8M8VB14_SESIN4.810e-6069.57ATPase GET3 n=1 Tax=Sesamum indicum TaxID=4182 Rep... [more]
A0A072TNB3_MEDTR5.220e-6070.29ATPase 25500968 n=5 Tax=IRL clade TaxID=2233839 Re... [more]
A0A5A7QM57_STRAF5.220e-6068.84ATPase n=3 Tax=Orobanchaceae TaxID=91896 RepID=A0A... [more]
A0A4D8XZB4_SALSN1.070e-5969.57ATPase arsA n=5 Tax=Salvia TaxID=21880 RepID=A0A4D... [more]
UPI0020097BAB1.170e-5968.12ATPase GET3A-like n=1 Tax=Salvia hispanica TaxID=4... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig561contigEcto-sp13_S_contig561:22571..23713 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeViridiplantae
Stop1
Start1
Seed ortholog score198.4
Seed ortholog evalue2.3e-48
Seed eggNOG ortholog3827.XP_004510885.1
Model size420
KEGG koko:K01551
KEGG TC3.A.19.1,3.A.21.1,3.A.4.1
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006996,GO:0007029,GO:0008104,GO:0008150,GO:0009987,GO:0010256,GO:0016043,GO:0033036,GO:0033365,GO:0034613,GO:0044424,GO:0044444,GO:0044464,GO:0045048,GO:0045184,GO:0051179,GO:0051205,GO:0051234,GO:0051641,GO:0061024,GO:0070727,GO:0071816,GO:0071840,GO:0072657,GO:0090150
Exons2
EggNOG free text desc.ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting
EggNOG OGs37MGZ@33090,3GGE0@35493,4JJET@91835,COG0003@1,KOG2825@2759
EC3.6.3.16
Cds size420
COG Functional cat.P
Best tax levelfabids
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko02000
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463778.414267-CDS-Ecto-sp13_S_contig561:22570..227801681463778.414267-CDS-Ecto-sp13_S_contig561:22570..22780Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig561 22571..22780 -
1681463778.4276261-CDS-Ecto-sp13_S_contig561:23503..237131681463778.4276261-CDS-Ecto-sp13_S_contig561:23503..23713Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig561 23504..23713 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig561.15943.1prot_Ecto-sp13_S_contig561.15943.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig561 22571..23713 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig561.15943.1

>prot_Ecto-sp13_S_contig561.15943.1 ID=prot_Ecto-sp13_S_contig561.15943.1|Name=mRNA_Ecto-sp13_S_contig561.15943.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=140bp
MSFAELMKMVQNMNFSCIVFDTAPTGHTLRLLSFPTIMDKALSKLMDLKN
KFSGLFSQFGNMFGAGGVSYATEDDMFGKLLQLKEIINKVNDQFKDPDKT
TFVCVCIPEFLSLYETERLVQELTKFEIDTHNIVVNQVC*
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mRNA from alignment at Ecto-sp13_S_contig561:22571..23713-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig561.15943.1 ID=mRNA_Ecto-sp13_S_contig561.15943.1|Name=mRNA_Ecto-sp13_S_contig561.15943.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1143bp|location=Sequence derived from alignment at Ecto-sp13_S_contig561:22571..23713- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCGTTCGCCGAGCTCATGAAGATGGTGCAGAACATGAACTTCAGCTG
CATCGTCTTCGACACGGCGCCTACGGGACACACCCTCCGGCTGCTCTCCT
TCCCGACCATCATGGACAAGGCGCTGTCGAAGCTGATGGACCTGAAGAAC
AAGTTCTCCGGCCTCTTCTCGCAGTTCGGGAACATGTTCGGAGCGGGCGG
GGTGAGTTATGTTGTCGGCAAACGGGGGTTGTATCGTGTTTTGCATGCCT
TTTTGTTTGTGGGACCGTCGACCTCGCGCCCTCGAGGGGACGGAGCGTTG
TCCTCAGAGGACCGATTATCGATAACTAGTATTCCGTCCCTGTTATGCAA
AAGGGTTCGGGGGCGGGGGGCGGGGAGGCATTGTCGTTGTCGTGCATGAC
CCTGCGTTGCCGGCCCTCAGGCGAGACCCCAGAAATGAGGTTTGGAGCAC
CAGCGGGCTGGAGGTACGAGCCGATCGGTTCTTTTGTTTTTGCTGAAGGT
GTTGTAAATATTTATGACTGAACGGAGACGACACAAGCCAGCATATTTAC
TGTCCGGCTACCCACGGGTTAAAGAAAACCGCCGTCGGCCAAGTAGAGAG
GCAGGTCGTTGCTGTGTCCCTCTTGAGTCAGGCTGTGGCAATTCAGAAGT
GGGGTGTGCATCAGGCTTGCTGGGAATCAGCGTTTGTCTCCTGATTTGGA
CCCCCAGGATTACCATTTTCGCGTTCATCGTCCATATACTTGCCGGGTTT
AACACAACGGTCCAAATTGGTTGTGTTCGCTTCTGGTACTCTACACGGAC
GCCGGCTTTTTTGTTGCAGTGCTTGTGTTCTGTAGGTCGGTGTTCGTCCT
TCCGGTCCCGTCCAATAAATGTTGTTTTGTGTTGTTCCCTGTATTGCCGC
ACCCTGTTCGTTCTATTCTTCCCCCACGCCCAGGCGACGGAGGACGACAT
GTTCGGGAAGCTGCTGCAGCTGAAGGAGATCATCAACAAGGTGAACGACC
AGTTCAAGGACCCGGACAAGACGACCTTCGTGTGTGTCTGCATCCCCGAG
TTCTTGTCCCTCTACGAGACCGAGAGGCTGGTGCAGGAGCTCACCAAGTT
CGAAATCGACACCCACAACATCGTCGTGAACCAGGTATGTTAG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig561:22571..23713-

>mRNA_Ecto-sp13_S_contig561.15943.1 ID=mRNA_Ecto-sp13_S_contig561.15943.1|Name=mRNA_Ecto-sp13_S_contig561.15943.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=420bp|location=Sequence derived from alignment at Ecto-sp13_S_contig561:22571..23713- (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTCGTTCGCCGAGCTCATGAAGATGGTGCAGAACATGAACTTCAGCTG
CATCGTCTTCGACACGGCGCCTACGGGACACACCCTCCGGCTGCTCTCCT
TCCCGACCATCATGGACAAGGCGCTGTCGAAGCTGATGGACCTGAAGAAC
AAGTTCTCCGGCCTCTTCTCGCAGTTCGGGAACATGTTCGGAGCGGGCGG
GGTGAGTTATGCGACGGAGGACGACATGTTCGGGAAGCTGCTGCAGCTGA
AGGAGATCATCAACAAGGTGAACGACCAGTTCAAGGACCCGGACAAGACG
ACCTTCGTGTGTGTCTGCATCCCCGAGTTCTTGTCCCTCTACGAGACCGA
GAGGCTGGTGCAGGAGCTCACCAAGTTCGAAATCGACACCCACAACATCG
TCGTGAACCAGGTATGTTAG
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