mRNA_Ecto-sp13_S_contig2022.6642.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig2022.6642.1
Unique NamemRNA_Ecto-sp13_S_contig2022.6642.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A6H5L214_9PHAE (Plug_translocon domain-containing protein n=3 Tax=PX clade TaxID=569578 RepID=A0A6H5L214_9PHAE)

HSP 1 Score: 165 bits (417), Expect = 4.370e-47
Identity = 90/90 (100.00%), Postives = 90/90 (100.00%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG
Sbjct:   82 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 171
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A7S2XXJ1_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XXJ1_9STRA)

HSP 1 Score: 156 bits (395), Expect = 7.430e-44
Identity = 85/90 (94.44%), Postives = 87/90 (96.67%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEVNQ++KEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDL TLGAGNAILII QLFFAGLIVIILDELLQKG
Sbjct:   82 IVTSGLVMQLLAGSRIIEVNQALKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLGTLGAGNAILIIAQLFFAGLIVIILDELLQKG 171
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A482RU87_9ARCH (Preprotein translocase subunit SecY n=2 Tax=cellular organisms TaxID=131567 RepID=A0A482RU87_9ARCH)

HSP 1 Score: 151 bits (382), Expect = 1.570e-42
Identity = 80/90 (88.89%), Postives = 87/90 (96.67%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            I+TS LVMQLLAGSRIIEVNQS+KEDRALF+GAQKLFGILITIGEA+AYVVSGMYGDL+TLGAGNAILII QLF AG++VIILDELLQKG
Sbjct:    9 IITSGLVMQLLAGSRIIEVNQSLKEDRALFNGAQKLFGILITIGEAIAYVVSGMYGDLATLGAGNAILIILQLFAAGMVVIILDELLQKG 98
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A6V2TM26_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V2TM26_HETAK)

HSP 1 Score: 151 bits (382), Expect = 5.940e-42
Identity = 78/90 (86.67%), Postives = 87/90 (96.67%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEVNQ +KEDRALF+GAQKLFGILIT+GEAVAYV+SGMYGDL+TLGAGNA+LII QLFFAGL+V++LDELLQKG
Sbjct:   82 IVTSGLVMQLLAGSRIIEVNQGVKEDRALFNGAQKLFGILITLGEAVAYVISGMYGDLATLGAGNALLIITQLFFAGLVVLLLDELLQKG 171
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A812V906_SYMMI (Sec61a protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812V906_SYMMI)

HSP 1 Score: 148 bits (374), Expect = 9.300e-42
Identity = 78/90 (86.67%), Postives = 86/90 (95.56%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEVNQS+KEDRALFSGAQKLFGILIT+GEA+AYVVSGMYGDL++LGAGNA+LII QL  AGL++IILDELLQKG
Sbjct:   82 IVTSGLVMQLLAGSRIIEVNQSLKEDRALFSGAQKLFGILITVGEAIAYVVSGMYGDLNSLGAGNALLIILQLIAAGLVIIILDELLQKG 171
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A7S1MN71_ALECA (Hypothetical protein (Fragment) n=1 Tax=Alexandrium catenella TaxID=2925 RepID=A0A7S1MN71_ALECA)

HSP 1 Score: 141 bits (355), Expect = 1.950e-41
Identity = 72/90 (80.00%), Postives = 85/90 (94.44%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            I+TS +VMQLLAGSRII+V+QS+KEDRALF GAQKLFG+LIT+GEAVAYV+SGMYGDL  LGAGN++LII QLFFAG+IVI+LDEL+QKG
Sbjct:   24 IITSGMVMQLLAGSRIIDVDQSLKEDRALFGGAQKLFGMLITMGEAVAYVMSGMYGDLRELGAGNSLLIIMQLFFAGIIVILLDELMQKG 113
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A0G4G804_VITBC (Plug_translocon domain-containing protein n=3 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4G804_VITBC)

HSP 1 Score: 148 bits (374), Expect = 8.880e-41
Identity = 79/90 (87.78%), Postives = 86/90 (95.56%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS +VMQLLAGS+II+V+QS+KEDRALFSGAQKLFG+LITIGEA+AYVVSGMYGDL  LGAGNAILII QLFFAGLIVIILDELLQKG
Sbjct:   86 IVTSGMVMQLLAGSKIIDVDQSLKEDRALFSGAQKLFGMLITIGEAIAYVVSGMYGDLKELGAGNAILIILQLFFAGLIVIILDELLQKG 175
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A7S4MCE0_9STRA (Hypothetical protein n=2 Tax=Bacillariophyta TaxID=2836 RepID=A0A7S4MCE0_9STRA)

HSP 1 Score: 148 bits (373), Expect = 1.220e-40
Identity = 77/90 (85.56%), Postives = 85/90 (94.44%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEVN ++KEDRALF GAQKLFGILIT+GEA+AYVVSGMYGDL+T+GAGNA+LII QLFFAGLIV+ LDELLQKG
Sbjct:   82 IVTSGLVMQLLAGSRIIEVNYNVKEDRALFQGAQKLFGILITVGEAIAYVVSGMYGDLATIGAGNALLIISQLFFAGLIVLTLDELLQKG 171
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A4D9D8Z0_9STRA (Plug_translocon domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D8Z0_9STRA)

HSP 1 Score: 147 bits (370), Expect = 3.350e-40
Identity = 80/90 (88.89%), Postives = 84/90 (93.33%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEVNQS+KEDRALFSGAQKLFGILITIGEAVAYV SGMYGDL++LG GNAILII QLF AG+IVI LDELLQKG
Sbjct:   84 IVTSGLVMQLLAGSRIIEVNQSLKEDRALFSGAQKLFGILITIGEAVAYVFSGMYGDLNSLGWGNAILIIAQLFIAGMIVITLDELLQKG 173
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BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Match: A0A7S1D8Y6_CYCTE (Hypothetical protein (Fragment) n=1 Tax=Cyclophora tenuis TaxID=216820 RepID=A0A7S1D8Y6_CYCTE)

HSP 1 Score: 144 bits (362), Expect = 6.730e-40
Identity = 76/90 (84.44%), Postives = 84/90 (93.33%), Query Frame = 1 
Query:    1 IVTSSLVMQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGDLSTLGAGNAILIICQLFFAGLIVIILDELLQKG 270
            IVTS LVMQLLAGSRIIEV+ ++KEDRALFSGAQKLFGILIT+GEA+AYVVSGMYGDL ++GAGNAILII QLF AGLIV+ LDELLQKG
Sbjct:   88 IVTSGLVMQLLAGSRIIEVDYNVKEDRALFSGAQKLFGILITVGEAIAYVVSGMYGDLGSIGAGNAILIIAQLFCAGLIVLTLDELLQKG 177
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig2022.6642.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5L214_9PHAE4.370e-47100.00Plug_translocon domain-containing protein n=3 Tax=... [more]
A0A7S2XXJ1_9STRA7.430e-4494.44Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A482RU87_9ARCH1.570e-4288.89Preprotein translocase subunit SecY n=2 Tax=cellul... [more]
A0A6V2TM26_HETAK5.940e-4286.67Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
A0A812V906_SYMMI9.300e-4286.67Sec61a protein n=1 Tax=Symbiodinium microadriaticu... [more]
A0A7S1MN71_ALECA1.950e-4180.00Hypothetical protein (Fragment) n=1 Tax=Alexandriu... [more]
A0A0G4G804_VITBC8.880e-4187.78Plug_translocon domain-containing protein n=3 Tax=... [more]
A0A7S4MCE0_9STRA1.220e-4085.56Hypothetical protein n=2 Tax=Bacillariophyta TaxID... [more]
A0A4D9D8Z0_9STRA3.350e-4088.89Plug_translocon domain-containing protein n=2 Tax=... [more]
A0A7S1D8Y6_CYCTE6.730e-4084.44Hypothetical protein (Fragment) n=1 Tax=Cyclophora... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig2022contigEcto-sp13_S_contig2022:13763..14933 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score154.5
Seed ortholog evalue2.3e-35
Seed eggNOG ortholog2880.D7FWL7
Preferred nameSEC61A1
Model size273
KEGG koko:K10956
KEGG TC3.A.5.4,3.A.5.8,3.A.5.9
KEGG Pathwayko03060,ko04141,ko04145,ko05110,map03060,map04141,map04145,map05110
KEGG ModuleM00401
Hectar predicted targeting categoryother localisation
GOsGO:0000322,GO:0000323,GO:0000324,GO:0001655,GO:0001700,GO:0001822,GO:0002009,GO:0002376,GO:0003674,GO:0005048,GO:0005215,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005783,GO:0005784,GO:0005789,GO:0005791,GO:0005829,GO:0005886,GO:0006508,GO:0006511,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006616,GO:0006620,GO:0006807,GO:0006810,GO:0006886,GO:0006950,GO:0006952,GO:0006955,GO:0006996,GO:0007029,GO:0007275,GO:0007391,GO:0007399,GO:0007417,GO:0007420,GO:0008104,GO:0008150,GO:0008152,GO:0008258,GO:0008320,GO:0008565,GO:0009056,GO:0009057,GO:0009605,GO:0009607,GO:0009615,GO:0009653,GO:0009790,GO:0009792,GO:0009888,GO:0009892,GO:0009894,GO:0009895,GO:0009987,GO:0010033,GO:0010243,GO:0010256,GO:0010498,GO:0010506,GO:0010507,GO:0010941,GO:0010942,GO:0010959,GO:0012505,GO:0012506,GO:0015031,GO:0015399,GO:0015405,GO:0015450,GO:0015833,GO:0016020,GO:0016021,GO:0016043,GO:0016331,GO:0017038,GO:0019222,GO:0019538,GO:0019941,GO:0021536,GO:0021538,GO:0021986,GO:0022804,GO:0022857,GO:0022884,GO:0030139,GO:0030163,GO:0030176,GO:0030433,GO:0030659,GO:0030666,GO:0030670,GO:0030867,GO:0030900,GO:0030970,GO:0031090,GO:0031204,GO:0031224,GO:0031227,GO:0031323,GO:0031324,GO:0031329,GO:0031330,GO:0031410,GO:0031982,GO:0031984,GO:0032501,GO:0032502,GO:0032527,GO:0032879,GO:0032991,GO:0033036,GO:0033218,GO:0033365,GO:0033554,GO:0034097,GO:0034341,GO:0034613,GO:0034756,GO:0034758,GO:0034759,GO:0034761,GO:0034762,GO:0034764,GO:0034765,GO:0034767,GO:0034976,GO:0036503,GO:0039019,GO:0042175,GO:0042221,GO:0042277,GO:0042335,GO:0042886,GO:0042887,GO:0043021,GO:0043022,GO:0043161,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043269,GO:0043270,GO:0043632,GO:0043900,GO:0043902,GO:0043903,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044433,GO:0044444,GO:0044446,GO:0044464,GO:0044743,GO:0044877,GO:0045047,GO:0045087,GO:0045169,GO:0045184,GO:0045335,GO:0046596,GO:0046598,GO:0046907,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048524,GO:0048598,GO:0048729,GO:0048731,GO:0048793,GO:0048856,GO:0050789,GO:0050792,GO:0050794,GO:0050896,GO:0051049,GO:0051050,GO:0051179,GO:0051234,GO:0051603,GO:0051641,GO:0051649,GO:0051704,GO:0051707,GO:0051716,GO:0055085,GO:0060322,GO:0060429,GO:0065002,GO:0065007,GO:0070727,GO:0070843,GO:0070972,GO:0071256,GO:0071261,GO:0071702,GO:0071704,GO:0071705,GO:0071806,GO:0071840,GO:0071944,GO:0072001,GO:0072006,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:0097708,GO:0098586,GO:0098588,GO:0098796,GO:0098805,GO:0098827,GO:1901564,GO:1901565,GO:1901575,GO:1901698,GO:1903513,GO:1903900,GO:1903902,GO:1904062,GO:1904064,GO:1904680
Exons2
EggNOG free text desc.protein transport
EggNOG OGsCOG0201@1,KOG1373@2759
Cds size252
COG Functional cat.U
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02044
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463016.5651124-UTR-Ecto-sp13_S_contig2022:13762..137831681463016.5651124-UTR-Ecto-sp13_S_contig2022:13762..13783Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig2022 13763..13783 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681463016.577872-CDS-Ecto-sp13_S_contig2022:13783..138671681463016.577872-CDS-Ecto-sp13_S_contig2022:13783..13867Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig2022 13784..13867 +
1681463016.588365-CDS-Ecto-sp13_S_contig2022:14765..149331681463016.588365-CDS-Ecto-sp13_S_contig2022:14765..14933Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig2022 14766..14933 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig2022.6642.1prot_Ecto-sp13_S_contig2022.6642.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig2022 13784..14933 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig2022.6642.1

>prot_Ecto-sp13_S_contig2022.6642.1 ID=prot_Ecto-sp13_S_contig2022.6642.1|Name=mRNA_Ecto-sp13_S_contig2022.6642.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=84bp
MQLLAGSRIIEVNQSIKEDRALFSGAQKLFGILITIGEAVAYVVSGMYGD
LSTLGAGNAILIICQLFFAGLIVIILDELLQKG*
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mRNA from alignment at Ecto-sp13_S_contig2022:13763..14933+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig2022.6642.1 ID=mRNA_Ecto-sp13_S_contig2022.6642.1|Name=mRNA_Ecto-sp13_S_contig2022.6642.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=1171bp|location=Sequence derived from alignment at Ecto-sp13_S_contig2022:13763..14933+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATCGTGACGTCATCACTCGTTATGCAGCTGCTCGCCGGGTCCCGAATCAT
CGAGGTCAACCAGTCCATCAAGGAAGATCGGGCTCTGTTCAGCGGAGCGC
AGAAGGTGAGCCAGCAGCACTGCAAGGAGAGAGGAGGGGGTCTGTGGTTT
GATGTCGTGGATGGGCGGTATTTACGACCTTATCCCCCCCCCCCCCCCCC
CCCCCCCCCCCCCCCCCCCGCGCGCGCGGCGCGGGGGCCCTGCGCGGGGC
GGGTGGTTTTTTTTGTCGGTGGGTGAGTTTTTTTGTGGGATTNNNNNNNN
NNGGAGGGGTGGTGTGGTTGGTTGTTGTGGGTGGGCGGTTTTTACCACCT
TATCCCCCCCCCCCCCCCGTCGTGCCTCCCCTGTCGGGGGTTCGCGGCTA
AGGCTGCCTGTCGGACGGTGTTCGTTGTTCCTAAACGGGCATGGTTTTTC
CTGAGAGCAATATATTATTTTCAGGTGGTTGGTTGATTGGTTGGTGGACG
TGCGGGGGTGTTTACTCCAGGACCACCCGTGAGAGCATTCGACCGAGAGG
TGGCGGGCAGCAAAACCAAAGGAGGGTGTGTTGATGAAACAGCTGGACAG
TTGGGAGATCGGGAGGGAGGACGACCCGGTAGCGAAGTTGTACGAACTCG
AGCATGCTCTCCTTGAAGCTAGATGCCGCTGGTACCAGAGATATCTTGTA
CCAGCGGCATCGAGCTTCAAGGAAAGCATTCCTGAGTTCGTAAACCTTCG
ACTCCCAGCCGATCTTCAGAATTGTCTAGCTGCTTCATCCGCACACCCTT
CTGGCTGTTGCTCTCCTCCTACGTGTATAATCCTGGAGCGCCAACCCTAG
CTAGATAGCCGTTCGAGTGCAAACTCTGATGGTGGCCGGCGCCCGTCCAA
TGACCGAAATTCTGAAGCGGGTATCTACCAGCGAAAACGCCCAACAAAAT
CTGCTGTGAAACCCCCCCCCCTGAAATATAATGATTCCGTCGCCCTTTCC
CAGCTCTTCGGTATCTTGATCACGATCGGAGAGGCCGTGGCGTACGTGGT
GTCTGGCATGTACGGTGACCTGTCTACCCTCGGTGCCGGTAACGCCATCC
TCATCATCTGCCAGCTCTTCTTCGCTGGGCTTATCGTTATCATCCTCGAC
GAGCTGCTGCAAAAGGGGTGA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig2022:13763..14933+

>mRNA_Ecto-sp13_S_contig2022.6642.1 ID=mRNA_Ecto-sp13_S_contig2022.6642.1|Name=mRNA_Ecto-sp13_S_contig2022.6642.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=252bp|location=Sequence derived from alignment at Ecto-sp13_S_contig2022:13763..14933+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCAGCTGCTCGCCGGGTCCCGAATCATCGAGGTCAACCAGTCCATCAA
GGAAGATCGGGCTCTGTTCAGCGGAGCGCAGAAGCTCTTCGGTATCTTGA
TCACGATCGGAGAGGCCGTGGCGTACGTGGTGTCTGGCATGTACGGTGAC
CTGTCTACCCTCGGTGCCGGTAACGCCATCCTCATCATCTGCCAGCTCTT
CTTCGCTGGGCTTATCGTTATCATCCTCGACGAGCTGCTGCAAAAGGGGT
GA
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