mRNA_Ecto-sp13_S_contig19698.6353.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig19698.6353.1
Unique NamemRNA_Ecto-sp13_S_contig19698.6353.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: D7FL84_ECTSI (Similar to aldehyde oxidase 3 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FL84_ECTSI)

HSP 1 Score: 192 bits (488), Expect = 7.220e-62
Identity = 94/105 (89.52%), Postives = 98/105 (93.33%), Query Frame = 1 
Query:    1 MPTGNDDRVDDSQKQGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPVGPFTTSGVAVYFRD 315
            MPTGNDD   D+Q  GTR+HL+FFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPVGP TTSGV V+FRD
Sbjct:    1 MPTGNDDAAGDTQ--GTRNHLVFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPVGPLTTSGVVVFFRD 103
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A6H5JRC2_9PHAE (Xanthine dehydrogenase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JRC2_9PHAE)

HSP 1 Score: 174 bits (441), Expect = 1.610e-48
Identity = 83/91 (91.21%), Postives = 87/91 (95.60%), Query Frame = 1 
Query:    1 MPTGNDDRVDDSQKQGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            MPTG+DD  DD+Q QGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRA GLTGTKLGCGEGGCGACTVMVSSFD++KKQIKHAAVNACLAPV
Sbjct:    1 MPTGHDDAADDTQNQGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAVGLTGTKLGCGEGGCGACTVMVSSFDAEKKQIKHAAVNACLAPV 91
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: D7G4V3_ECTSI (Xanthine dehydrogenase n=3 Tax=Ectocarpus TaxID=2879 RepID=D7G4V3_ECTSI)

HSP 1 Score: 161 bits (408), Expect = 6.380e-44
Identity = 79/91 (86.81%), Postives = 82/91 (90.11%), Query Frame = 1 
Query:    1 MPTGNDDRVDDSQKQGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            MPT ND   DDSQK   RDHL+FFVNGAKQVVKDAQPQTTLLQHLRA GLTGTKLGCGEGGCGACTVMVSSFDS+KKQI HAAVNACLAP+
Sbjct:    1 MPTENDYGADDSQKGTCRDHLVFFVNGAKQVVKDAQPQTTLLQHLRAVGLTGTKLGCGEGGCGACTVMVSSFDSEKKQITHAAVNACLAPM 91
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A836C8N1_9STRA (Xanthine dehydrogenase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C8N1_9STRA)

HSP 1 Score: 127 bits (319), Expect = 6.390e-32
Identity = 59/74 (79.73%), Postives = 66/74 (89.19%), Query Frame = 1 
Query:   52 RDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            RDHL+FFVNG +QVVKDAQP+ TLL  LRA+GLTGTKLGCGEGGCGACTVMVSS+D   K I+HAAVNACLAP+
Sbjct:    6 RDHLVFFVNGKRQVVKDAQPEVTLLSWLRASGLTGTKLGCGEGGCGACTVMVSSYDHGAKTIRHAAVNACLAPI 79
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: UPI00052268C1 (xanthine dehydrogenase/oxidase-like n=1 Tax=Leptosomus discolor TaxID=188344 RepID=UPI00052268C1)

HSP 1 Score: 100 bits (250), Expect = 1.270e-24
Identity = 49/75 (65.33%), Postives = 56/75 (74.67%), Query Frame = 1 
Query:   52 RDHLIFFVNGAKQVVKDAQPQTTLLQHLRAA-GLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            RD L+FFVNG K V KD  P+TTLL +LR   GL GTKLGCGEGGCGACTVM+S +D  +K+I H   NACL PV
Sbjct:    7 RDELVFFVNGKKVVEKDVDPETTLLTYLRRKLGLCGTKLGCGEGGCGACTVMISKYDPFRKKILHHTANACLFPV 81
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A8B7S8X3_HIPAR (xanthine dehydrogenase/oxidase-like n=1 Tax=Hipposideros armiger TaxID=186990 RepID=A0A8B7S8X3_HIPAR)

HSP 1 Score: 101 bits (251), Expect = 1.420e-24
Identity = 49/76 (64.47%), Postives = 60/76 (78.95%), Query Frame = 1 
Query:   49 TRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAA-GLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            T D L+FFVNG K V K+A P+TTLL +LR + GL+GTKLGCGEGGCGACTVMVS +D  + +I H +VNACL P+
Sbjct:    2 TADELVFFVNGRKVVEKNADPETTLLAYLRRSLGLSGTKLGCGEGGCGACTVMVSKYDRLQNKIIHFSVNACLTPI 77
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A8B7TCL7_HIPAR (xanthine dehydrogenase/oxidase-like n=1 Tax=Hipposideros armiger TaxID=186990 RepID=A0A8B7TCL7_HIPAR)

HSP 1 Score: 98.6 bits (244), Expect = 2.410e-24
Identity = 48/76 (63.16%), Postives = 59/76 (77.63%), Query Frame = 1 
Query:   49 TRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAA-GLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            T D L+FFVNG K V K+A P+TTLL +LR + GL+GTKLGCG GGCGACTVMVS +D  + +I H +VNACL P+
Sbjct:    2 TADKLVFFVNGRKVVEKNADPETTLLAYLRRSLGLSGTKLGCGVGGCGACTVMVSKYDHLQNKIVHFSVNACLTPI 77
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A6P4V651_PANPR (xanthine dehydrogenase/oxidase-like isoform X3 n=2 Tax=Panthera pardus TaxID=9691 RepID=A0A6P4V651_PANPR)

HSP 1 Score: 100 bits (250), Expect = 2.770e-24
Identity = 49/76 (64.47%), Postives = 60/76 (78.95%), Query Frame = 1 
Query:   49 TRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAA-GLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            T D L+FFVNG K V K+A P+TTLL +LR   GL+GTKLGCGEGGCGACTVM+S +D  + +I H +VNACLAP+
Sbjct:    2 TVDELVFFVNGKKVVEKNADPETTLLSYLRRKLGLSGTKLGCGEGGCGACTVMLSKYDRFQNKIIHFSVNACLAPI 77
[Close]
BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: A0A1Y1YDJ4_9FUNG (Xanthine dehydrogenase n=2 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1YDJ4_9FUNG)

HSP 1 Score: 105 bits (262), Expect = 3.140e-24
Identity = 47/77 (61.04%), Postives = 61/77 (79.22%), Query Frame = 1 
Query:   43 QGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            Q   D L F+VNG +  +++  P+ TLLQ+LR AGLTGTKLGCGEGGCGACT++VSS+D D ++I H +VNACLAP+
Sbjct:    4 QSYSDTLTFYVNGTRVALRNPDPEMTLLQYLRHAGLTGTKLGCGEGGCGACTLLVSSYDPDTRKISHTSVNACLAPL 80
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BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Match: UPI000529F83E (xanthine dehydrogenase/oxidase-like n=1 Tax=Colius striatus TaxID=57412 RepID=UPI000529F83E)

HSP 1 Score: 99.8 bits (247), Expect = 4.050e-24
Identity = 48/76 (63.16%), Postives = 56/76 (73.68%), Query Frame = 1 
Query:   49 TRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAA-GLTGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPV 273
            T D L+FFVNG K V KD  P+TTLL +LR   GL GTKLGCGEGGCGACTVM+S +D  +K+I H   NACL P+
Sbjct:    6 TGDELVFFVNGKKVVEKDVDPETTLLTYLRRKLGLCGTKLGCGEGGCGACTVMISKYDPFRKKILHHTANACLFPI 81
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig19698.6353.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FL84_ECTSI7.220e-6289.52Similar to aldehyde oxidase 3 n=1 Tax=Ectocarpus s... [more]
A0A6H5JRC2_9PHAE1.610e-4891.21Xanthine dehydrogenase n=1 Tax=Ectocarpus sp. CCAP... [more]
D7G4V3_ECTSI6.380e-4486.81Xanthine dehydrogenase n=3 Tax=Ectocarpus TaxID=28... [more]
A0A836C8N1_9STRA6.390e-3279.73Xanthine dehydrogenase n=1 Tax=Tribonema minus Tax... [more]
UPI00052268C11.270e-2465.33xanthine dehydrogenase/oxidase-like n=1 Tax=Leptos... [more]
A0A8B7S8X3_HIPAR1.420e-2464.47xanthine dehydrogenase/oxidase-like n=1 Tax=Hippos... [more]
A0A8B7TCL7_HIPAR2.410e-2463.16xanthine dehydrogenase/oxidase-like n=1 Tax=Hippos... [more]
A0A6P4V651_PANPR2.770e-2464.47xanthine dehydrogenase/oxidase-like isoform X3 n=2... [more]
A0A1Y1YDJ4_9FUNG3.140e-2461.04Xanthine dehydrogenase n=2 Tax=Basidiobolus merist... [more]
UPI000529F83E4.050e-2463.16xanthine dehydrogenase/oxidase-like n=1 Tax=Colius... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig19698contigEcto-sp13_S_contig19698:1591..2318 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start1
Seed ortholog score194.9
Seed ortholog evalue1.9e-47
Seed eggNOG ortholog2880.D7FL84
Preferred nameXDH
Model size315
KEGG rclassRC00143,RC02017,RC02199
KEGG koko:K00106
KEGG ReactionR01768,R01769,R02103,R02107,R07942,R07977,R07978,R07979,R08235
KEGG Pathwayko00230,ko00232,ko00983,ko01100,ko01110,ko01120,ko04146,map00230,map00232,map00983,map01100,map01110,map01120,map04146
KEGG ModuleM00546
Hectar predicted targeting categoryother localisation
GOsGO:0000166,GO:0000302,GO:0001932,GO:0001933,GO:0001934,GO:0001936,GO:0001937,GO:0002197,GO:0002237,GO:0003674,GO:0003824,GO:0004854,GO:0004855,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005615,GO:0005622,GO:0005623,GO:0005737,GO:0005777,GO:0005783,GO:0005829,GO:0006082,GO:0006139,GO:0006144,GO:0006145,GO:0006150,GO:0006163,GO:0006195,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006919,GO:0006950,GO:0006979,GO:0007275,GO:0007568,GO:0007589,GO:0007595,GO:0008150,GO:0008152,GO:0008285,GO:0009056,GO:0009058,GO:0009112,GO:0009114,GO:0009115,GO:0009117,GO:0009166,GO:0009266,GO:0009408,GO:0009605,GO:0009607,GO:0009617,GO:0009628,GO:0009636,GO:0009892,GO:0009893,GO:0009966,GO:0009967,GO:0009968,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0010044,GO:0010468,GO:0010562,GO:0010563,GO:0010604,GO:0010605,GO:0010629,GO:0010646,GO:0010647,GO:0010648,GO:0010941,GO:0010942,GO:0010950,GO:0010952,GO:0012505,GO:0016491,GO:0016528,GO:0016529,GO:0016661,GO:0016662,GO:0016725,GO:0016726,GO:0016727,GO:0016903,GO:0018130,GO:0019220,GO:0019222,GO:0019438,GO:0019439,GO:0019637,GO:0022603,GO:0023051,GO:0023056,GO:0023057,GO:0030151,GO:0030162,GO:0030856,GO:0030857,GO:0030879,GO:0031323,GO:0031324,GO:0031325,GO:0031399,GO:0031400,GO:0031401,GO:0032268,GO:0032269,GO:0032270,GO:0032496,GO:0032501,GO:0032502,GO:0032872,GO:0032874,GO:0032991,GO:0033993,GO:0034097,GO:0034404,GO:0034418,GO:0034465,GO:0034612,GO:0034641,GO:0034654,GO:0034655,GO:0036094,GO:0042127,GO:0042221,GO:0042325,GO:0042326,GO:0042327,GO:0042493,GO:0042542,GO:0042579,GO:0042802,GO:0042803,GO:0042981,GO:0043065,GO:0043067,GO:0043068,GO:0043085,GO:0043167,GO:0043168,GO:0043169,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043280,GO:0043281,GO:0043408,GO:0043410,GO:0043546,GO:0044093,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044270,GO:0044271,GO:0044281,GO:0044283,GO:0044421,GO:0044424,GO:0044444,GO:0044464,GO:0045595,GO:0045596,GO:0045601,GO:0045602,GO:0045862,GO:0045936,GO:0045937,GO:0046100,GO:0046110,GO:0046113,GO:0046415,GO:0046434,GO:0046483,GO:0046677,GO:0046700,GO:0046872,GO:0046903,GO:0046914,GO:0046983,GO:0048037,GO:0048513,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048583,GO:0048584,GO:0048585,GO:0048731,GO:0048732,GO:0048856,GO:0050421,GO:0050660,GO:0050662,GO:0050678,GO:0050680,GO:0050789,GO:0050790,GO:0050793,GO:0050794,GO:0050878,GO:0050896,GO:0051093,GO:0051171,GO:0051172,GO:0051173,GO:0051174,GO:0051179,GO:0051234,GO:0051239,GO:0051241,GO:0051246,GO:0051247,GO:0051248,GO:0051336,GO:0051345,GO:0051536,GO:0051537,GO:0051540,GO:0051704,GO:0051707,GO:0051716,GO:0051896,GO:0051898,GO:0052547,GO:0052548,GO:0055086,GO:0055114,GO:0060255,GO:0065007,GO:0065008,GO:0065009,GO:0070302,GO:0070304,GO:0070555,GO:0070674,GO:0070887,GO:0071310,GO:0071345,GO:0071347,GO:0071356,GO:0071704,GO:0071949,GO:0072521,GO:0072522,GO:0072523,GO:0080090,GO:0080134,GO:0080135,GO:0090287,GO:0090288,GO:0097159,GO:0097184,GO:0098809,GO:1900744,GO:1900745,GO:1900746,GO:1900747,GO:1901265,GO:1901292,GO:1901342,GO:1901343,GO:1901360,GO:1901361,GO:1901362,GO:1901363,GO:1901564,GO:1901565,GO:1901566,GO:1901575,GO:1901576,GO:1901698,GO:1901700,GO:1902494,GO:1902531,GO:1902532,GO:1902533,GO:1902547,GO:1902548,GO:2000026,GO:2000116,GO:2000181,GO:2000377,GO:2000379,GO:2001056,GO:2001212,GO:2001213
Exons3
EggNOG free text desc.Xanthine dehydrogenase
EggNOG OGsCOG4631@1,KOG0430@2759
Ec32 ortholog description2Fe-2S ferredoxin-type domain
Ec32 orthologEc-00_002570.1
EC1.17.1.4,1.17.3.2
Cds size315
COG Functional cat.F
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462994.6291544-CDS-Ecto-sp13_S_contig19698:1590..17451681462994.6291544-CDS-Ecto-sp13_S_contig19698:1590..1745Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19698 1591..1745 +
1681462994.6427143-CDS-Ecto-sp13_S_contig19698:1951..20691681462994.6427143-CDS-Ecto-sp13_S_contig19698:1951..2069Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19698 1952..2069 +
1681462994.6523738-CDS-Ecto-sp13_S_contig19698:2276..23181681462994.6523738-CDS-Ecto-sp13_S_contig19698:2276..2318Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig19698 2277..2318 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig19698.6353.1prot_Ecto-sp13_S_contig19698.6353.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig19698 1591..2318 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig19698.6353.1

>prot_Ecto-sp13_S_contig19698.6353.1 ID=prot_Ecto-sp13_S_contig19698.6353.1|Name=mRNA_Ecto-sp13_S_contig19698.6353.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=105bp
MPTGNDDRVDDSQKQGTRDHLIFFVNGAKQVVKDAQPQTTLLQHLRAAGL
TGTKLGCGEGGCGACTVMVSSFDSDKKQIKHAAVNACLAPVGPFTTSGVA
VYFRD
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mRNA from alignment at Ecto-sp13_S_contig19698:1591..2318+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig19698.6353.1 ID=mRNA_Ecto-sp13_S_contig19698.6353.1|Name=mRNA_Ecto-sp13_S_contig19698.6353.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=728bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19698:1591..2318+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCCGACGGGGAATGATGATAGGGTCGATGACAGCCAGAAGCAGGGGAC
CAGAGACCACCTAATCTTCTTCGTGAATGGAGCTAAGCAGGTGGTCAAAG
ACGCCCAGCCTCAGACGACACTGCTGCAACATCTCCGGGCGGCCGGCCTG
ACCGGGTGAGGCTGCGGCGCAAAAGAGTCGGTGCGCTTGCGGCGTGTAGA
GTCTGGACGCGACACGTAGATAGCGGTAAGTTGCAGTTTTGAGGGAGCCA
GATGAGGAGTTCAATTAGTGCGCAATTCATATGCAACACGCTCACTGATG
TCCCTTTCGACGTGCCCCGCTGCTTTGTCGGACGTACTTACGATCGCGCT
GCCGTAAGCAGGACCAAGCTTGGATGTGGGGAGGGTGGATGTGGGGCATG
CACGGTGATGGTGTCTTCCTTCGACTCGGACAAGAAGCAGATCAAGCACG
CCGCAGTCAATGCCTGCCTGGCGCCCGTGGTAGGTGCCTCTTCCTTTGCC
TGCCGGAGACGTTCACGCGACAGCAGAAGTGTAGTGCTCTCTCTCTCTCT
CTGTGTGTGTGTGTGTGTCTCTGTCTCTGGTCTCTTGTCTCTTGTCTCTT
TCGAATTCCACACGTGCATATAATCTCTAGTGCTGAATGCATGTCTGAGC
CATATCATGATCCTGTTTTGTGTTCCCGAACATGAGGGACCATTTACGAC
AAGCGGGGTGGCGGTATATTTTAGAGAT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig19698:1591..2318+

>mRNA_Ecto-sp13_S_contig19698.6353.1 ID=mRNA_Ecto-sp13_S_contig19698.6353.1|Name=mRNA_Ecto-sp13_S_contig19698.6353.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=315bp|location=Sequence derived from alignment at Ecto-sp13_S_contig19698:1591..2318+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGCCGACGGGGAATGATGATAGGGTCGATGACAGCCAGAAGCAGGGGAC
CAGAGACCACCTAATCTTCTTCGTGAATGGAGCTAAGCAGGTGGTCAAAG
ACGCCCAGCCTCAGACGACACTGCTGCAACATCTCCGGGCGGCCGGCCTG
ACCGGGACCAAGCTTGGATGTGGGGAGGGTGGATGTGGGGCATGCACGGT
GATGGTGTCTTCCTTCGACTCGGACAAGAAGCAGATCAAGCACGCCGCAG
TCAATGCCTGCCTGGCGCCCGTGGGACCATTTACGACAAGCGGGGTGGCG
GTATATTTTAGAGAT
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