mRNA_Ecto-sp13_S_contig18333.5694.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig18333.5694.1
Unique NamemRNA_Ecto-sp13_S_contig18333.5694.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: D8LD49_ECTSI (DNA polymerase epsilon subunit n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LD49_ECTSI)

HSP 1 Score: 186 bits (471), Expect = 1.460e-54
Identity = 88/96 (91.67%), Postives = 88/96 (91.67%), Query Frame = 1 
Query:    1 PPPPRTSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 288
            P  P    T H CTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD
Sbjct:  424 PSDPDMDVTEH-CTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 518
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A7S3XUX9_HETAK (DNA polymerase II subunit 2 n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XUX9_HETAK)

HSP 1 Score: 129 bits (325), Expect = 6.530e-35
Identity = 57/90 (63.33%), Postives = 66/90 (73.33%), Query Frame = 1 
Query:   19 SQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 288
            S        TLL QGHLCPLPL ARPIHW+ D AL LYPLPH  VLAD+T+QY+W   DC + NPGSFP+D SF+VY PAT+ VEFS +D
Sbjct:  203 SDATETLVATLLAQGHLCPLPLPARPIHWKADPALQLYPLPHAVVLADQTDQYKWTYEDCHAFNPGSFPTDFSFIVYTPATQDVEFSKID 292
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A835YQA3_9STRA (DNA polymerase epsilon subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YQA3_9STRA)

HSP 1 Score: 115 bits (289), Expect = 2.920e-28
Identity = 56/94 (59.57%), Postives = 67/94 (71.28%), Query Frame = 1 
Query:    1 PPPPRTSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCC-SLNPGSFPSDSSFVVYRPATRTVEFS 279
            PP       +    KTLL QGHLCPLPLS+RPIH + D AL LYPLP V +LADR+EQ+ W  +D   ++NPGSFPSD SFVVYRP++  VEFS
Sbjct:  429 PPDAPNMDASEHLVKTLLDQGHLCPLPLSSRPIHCQFDAALRLYPLPQVVILADRSEQFSWQYHDSTWAINPGSFPSDFSFVVYRPSSGDVEFS 522
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A7S2WES5_9STRA (DNA polymerase II subunit 2 (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WES5_9STRA)

HSP 1 Score: 105 bits (263), Expect = 1.480e-27
Identity = 48/88 (54.55%), Postives = 64/88 (72.73%), Query Frame = 1 
Query:    1 PPPPRTSQTNHQC-TKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPAT 261
            PP P T  T  +C  ++LL QGHLCPLPL+ RPIHW  D AL L+P+P V +LAD ++QY     DC ++NPGSFP+D SF+VY+P++
Sbjct:   28 PPAPETDAT--ECLVQSLLDQGHLCPLPLTTRPIHWSYDHALRLHPMPDVVILADHSDQYSCTYQDCLAVNPGSFPTDFSFLVYQPSS 113
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A7S2C1W0_9STRA (DNA polymerase II subunit 2 n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2C1W0_9STRA)

HSP 1 Score: 107 bits (266), Expect = 2.730e-27
Identity = 50/93 (53.76%), Postives = 62/93 (66.67%), Query Frame = 1 
Query:    7 PPRTSQTNHQCTK----TLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVE 273
            PPR+       T+    T+L QGHLCPLPL++RPIHW+ D AL LYP P + VLAD TE Y W   DC ++NPGSF S+ +F+VYRPA    E
Sbjct:   73 PPRSGDREMDITEHLVATVLDQGHLCPLPLTSRPIHWKYDHALRLYPHPTMVVLADHTEPYNWKYQDCLAINPGSFSSEFAFMVYRPADCDAE 165
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A0V0GIJ1_SOLCH (DNA polymerase II subunit 2 (Fragment) n=1 Tax=Solanum chacoense TaxID=4108 RepID=A0A0V0GIJ1_SOLCH)

HSP 1 Score: 94.4 bits (233), Expect = 4.370e-23
Identity = 44/94 (46.81%), Postives = 54/94 (57.45%), Query Frame = 1 
Query:    7 PPRTSQTN---HQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFS 279
            PP T +TN        T++HQ HLCPLPL  +PI W  D  L LYP PH  VL D++EQ  +        NPGSF +D +FV YRP  + VE S
Sbjct:   14 PPSTEETNDPFEHLVATIIHQSHLCPLPLMVQPIIWNYDHGLHLYPTPHTIVLGDKSEQKAFRYTGVTCFNPGSFSNDGTFVAYRPCNQEVELS 107
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A1V9YX49_9STRA (DNA polymerase epsilon subunit n=1 Tax=Achlya hypogyna TaxID=1202772 RepID=A0A1V9YX49_9STRA)

HSP 1 Score: 101 bits (251), Expect = 5.520e-23
Identity = 46/92 (50.00%), Postives = 63/92 (68.48%), Query Frame = 1 
Query:   13 RTSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 288
            RT+  + Q  KT++ QGHLCPL LSA+PIHW  D +L L+PLP V VLAD+T+Q++       +++PG F  D SF++YRPAT   E S +D
Sbjct:  432 RTTTVSRQLVKTVIDQGHLCPLSLSAQPIHWAYDGSLQLFPLPDVLVLADKTDQFQVGYAGVAAIHPGPFYVDYSFMLYRPATAVTELSRID 523
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A1V9Z0I7_9STRA (DNA polymerase II subunit 2 (Fragment) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9Z0I7_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 7.060e-22
Identity = 43/92 (46.74%), Postives = 62/92 (67.39%), Query Frame = 1 
Query:   13 RTSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 288
            R +  + Q  KT++ QGHLCPLPLSA+P+HW  D++L L+PLP V  LAD+T+QY+       + +PG F  D SF++YRP++   E S +D
Sbjct:  312 RMTTVSRQLVKTIVDQGHLCPLPLSAQPVHWAFDNSLHLFPLPDVICLADKTDQYQVGYAGVTAFHPGPFYVDYSFMLYRPSSGVAEISSID 403
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A1R3HTF3_9ROSI (DNA polymerase II subunit 2 n=1 Tax=Corchorus olitorius TaxID=93759 RepID=A0A1R3HTF3_9ROSI)

HSP 1 Score: 97.4 bits (241), Expect = 9.080e-22
Identity = 45/96 (46.88%), Postives = 56/96 (58.33%), Query Frame = 1 
Query:    7 PPRTSQTN---HQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHV 285
            PP T +T+        T++HQ HLCPLPL  +PI W  D  L LYP PH  +L DR+EQ  +        NPGSF  D +FV YRP T+ +EFS V
Sbjct:  342 PPSTEETDDPFEHLVATIIHQSHLCPLPLFVQPIIWNYDHGLQLYPTPHTIILGDRSEQKAFKYTGITCFNPGSFSDDGTFVAYRPCTQEIEFSAV 437
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BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Match: A0A024TMJ7_9STRA (DNA polymerase epsilon subunit n=2 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024TMJ7_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 1.310e-21
Identity = 44/91 (48.35%), Postives = 59/91 (64.84%), Query Frame = 1 
Query:   16 TSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVAVLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD 288
            ++  +    KTL+ QGHLCPLP+ ARPIHW  D +L L+PLP V +LAD+ +Q++         +PG F  D SFV+YRP+T T E S VD
Sbjct:  430 SNDVSRHLVKTLVDQGHLCPLPIPARPIHWAYDSSLQLFPLPAVLILADKCDQFQVNYAGVSVFHPGPFHVDYSFVLYRPSTNTAEISRVD 520
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig18333.5694.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LD49_ECTSI1.460e-5491.67DNA polymerase epsilon subunit n=1 Tax=Ectocarpus ... [more]
A0A7S3XUX9_HETAK6.530e-3563.33DNA polymerase II subunit 2 n=1 Tax=Heterosigma ak... [more]
A0A835YQA3_9STRA2.920e-2859.57DNA polymerase epsilon subunit n=1 Tax=Tribonema m... [more]
A0A7S2WES5_9STRA1.480e-2754.55DNA polymerase II subunit 2 (Fragment) n=1 Tax=Rhi... [more]
A0A7S2C1W0_9STRA2.730e-2753.76DNA polymerase II subunit 2 n=1 Tax=Florenciella p... [more]
A0A0V0GIJ1_SOLCH4.370e-2346.81DNA polymerase II subunit 2 (Fragment) n=1 Tax=Sol... [more]
A0A1V9YX49_9STRA5.520e-2350.00DNA polymerase epsilon subunit n=1 Tax=Achlya hypo... [more]
A0A1V9Z0I7_9STRA7.060e-2246.74DNA polymerase II subunit 2 (Fragment) n=1 Tax=Thr... [more]
A0A1R3HTF3_9ROSI9.080e-2246.88DNA polymerase II subunit 2 n=1 Tax=Corchorus olit... [more]
A0A024TMJ7_9STRA1.310e-2148.35DNA polymerase epsilon subunit n=2 Tax=Aphanomyces... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig18333contigEcto-sp13_S_contig18333:3140..3430 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start0
Seed ortholog score187.6
Seed ortholog evalue2.8e-45
Seed eggNOG ortholog2880.D8LD49
Preferred namePOLE2
Model size291
KEGG rclassRC00005,RC02795
KEGG koko:K02325,ko:K10777
KEGG ReactionR00375,R00376,R00377,R00378,R00381
KEGG Pathwayko00230,ko00240,ko01100,ko03030,ko03410,ko03420,ko03450,ko05166,map00230,map00240,map01100,map03030,map03410,map03420,map03450,map05166
KEGG ModuleM00263
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000082,GO:0000228,GO:0000278,GO:0000731,GO:0000785,GO:0000790,GO:0003006,GO:0003674,GO:0003824,GO:0003887,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005654,GO:0005657,GO:0005694,GO:0005737,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006270,GO:0006275,GO:0006281,GO:0006301,GO:0006325,GO:0006342,GO:0006355,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007049,GO:0007275,GO:0008150,GO:0008152,GO:0008622,GO:0009058,GO:0009059,GO:0009790,GO:0009791,GO:0009793,GO:0009889,GO:0009890,GO:0009891,GO:0009892,GO:0009893,GO:0009987,GO:0010154,GO:0010468,GO:0010556,GO:0010557,GO:0010558,GO:0010564,GO:0010604,GO:0010605,GO:0010629,GO:0016043,GO:0016458,GO:0016604,GO:0016740,GO:0016772,GO:0016779,GO:0018130,GO:0019219,GO:0019222,GO:0019438,GO:0019899,GO:0019985,GO:0022402,GO:0022414,GO:0031261,GO:0031323,GO:0031324,GO:0031325,GO:0031326,GO:0031327,GO:0031328,GO:0031974,GO:0031981,GO:0032501,GO:0032502,GO:0032875,GO:0032877,GO:0032991,GO:0032993,GO:0033260,GO:0033554,GO:0034061,GO:0034401,GO:0034641,GO:0034645,GO:0034654,GO:0040029,GO:0042276,GO:0042575,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043596,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044271,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044446,GO:0044451,GO:0044454,GO:0044464,GO:0044770,GO:0044772,GO:0044786,GO:0044843,GO:0045005,GO:0045740,GO:0045787,GO:0045814,GO:0045892,GO:0045934,GO:0045935,GO:0046483,GO:0048316,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048608,GO:0048731,GO:0048856,GO:0050789,GO:0050794,GO:0050896,GO:0051052,GO:0051054,GO:0051171,GO:0051172,GO:0051173,GO:0051252,GO:0051253,GO:0051276,GO:0051302,GO:0051716,GO:0051726,GO:0051781,GO:0060255,GO:0061458,GO:0061695,GO:0065007,GO:0070013,GO:0070182,GO:0070828,GO:0070868,GO:0071704,GO:0071840,GO:0071897,GO:0080090,GO:0090068,GO:0090304,GO:0090329,GO:0097549,GO:0140097,GO:1901360,GO:1901362,GO:1901576,GO:1902292,GO:1902315,GO:1902494,GO:1902679,GO:1902969,GO:1902975,GO:1903047,GO:1903506,GO:1903507,GO:1990234,GO:2000105,GO:2000112,GO:2000113,GO:2001141
Exons1
EggNOG free text desc.error-prone translesion synthesis
EggNOG OGsKOG3818@1,KOG3818@2759
Ec32 ortholog descriptionDNA polymerase epsilon subunit B, N-terminal
Ec32 orthologEc-11_000450.3
EC2.7.7.7,6.5.1.1
Cds size291
COG Functional cat.K
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462944.271165-CDS-Ecto-sp13_S_contig18333:3139..34301681462944.271165-CDS-Ecto-sp13_S_contig18333:3139..3430Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig18333 3140..3430 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig18333.5694.1prot_Ecto-sp13_S_contig18333.5694.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig18333 3140..3430 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig18333.5694.1

>prot_Ecto-sp13_S_contig18333.5694.1 ID=prot_Ecto-sp13_S_contig18333.5694.1|Name=mRNA_Ecto-sp13_S_contig18333.5694.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=97bp
PPPPRTSQTNHQCTKTLLHQGHLCPLPLSARPIHWELDDALSLYPLPHVA
VLADRTEQYRWLLNDCCSLNPGSFPSDSSFVVYRPATRTVEFSHVD*
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mRNA from alignment at Ecto-sp13_S_contig18333:3140..3430-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig18333.5694.1 ID=mRNA_Ecto-sp13_S_contig18333.5694.1|Name=mRNA_Ecto-sp13_S_contig18333.5694.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=291bp|location=Sequence derived from alignment at Ecto-sp13_S_contig18333:3140..3430- (Ectocarpus species13 EcNAP12_S_4_19m)
CCCCCCCCCCCCCGTACCTCCCAAACCAACCATCAGTGCACGAAGACGCT
CCTTCACCAAGGGCACCTGTGTCCGCTCCCGCTATCGGCGCGGCCGATCC
ACTGGGAGCTGGACGACGCTCTGTCTCTGTACCCGCTCCCGCACGTGGCC
GTGCTCGCGGACAGGACCGAGCAGTACCGATGGTTGCTCAACGACTGCTG
CTCGCTCAACCCCGGCTCCTTCCCCAGCGACTCCTCGTTTGTGGTGTACC
GGCCCGCGACACGGACCGTCGAGTTCAGCCACGTGGACTAA
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig18333:3140..3430-

>mRNA_Ecto-sp13_S_contig18333.5694.1 ID=mRNA_Ecto-sp13_S_contig18333.5694.1|Name=mRNA_Ecto-sp13_S_contig18333.5694.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=291bp|location=Sequence derived from alignment at Ecto-sp13_S_contig18333:3140..3430- (Ectocarpus species13 EcNAP12_S_4_19m)
CCCCCCCCCCCCCGTACCTCCCAAACCAACCATCAGTGCACGAAGACGCT
CCTTCACCAAGGGCACCTGTGTCCGCTCCCGCTATCGGCGCGGCCGATCC
ACTGGGAGCTGGACGACGCTCTGTCTCTGTACCCGCTCCCGCACGTGGCC
GTGCTCGCGGACAGGACCGAGCAGTACCGATGGTTGCTCAACGACTGCTG
CTCGCTCAACCCCGGCTCCTTCCCCAGCGACTCCTCGTTTGTGGTGTACC
GGCCCGCGACACGGACCGTCGAGTTCAGCCACGTGGACTAA
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