mRNA_Ecto-sp13_S_contig17212.5107.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig17212.5107.1
Unique NamemRNA_Ecto-sp13_S_contig17212.5107.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: D8LTY7_ECTSI (Malate synthase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LTY7_ECTSI)

HSP 1 Score: 248 bits (632), Expect = 4.570e-76
Identity = 121/124 (97.58%), Postives = 122/124 (98.39%), Query Frame = 1 
Query:  271 QHGLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGG-GGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKISHGRL 639
            QHGLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLL+ASGG GGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIV ISHGRL
Sbjct:  411 QHGLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLSASGGDGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVNISHGRL 534
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A485LAT4_9STRA (Malate synthase n=2 Tax=Aphanomyces TaxID=100860 RepID=A0A485LAT4_9STRA)

HSP 1 Score: 130 bits (327), Expect = 1.470e-31
Identity = 66/114 (57.89%), Postives = 80/114 (70.18%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIV 618
            GLEEN++V + YVE+WLRGNGCIP++ KMEDAATAEISR QVWQW+ H  STS+G  IT  LV DI    T K L A   G  K+ LA  +TA+++T P   +FLT   YPHIV
Sbjct:  415 GLEENIDVTLVYVEAWLRGNGCIPLHNKMEDAATAEISRVQVWQWLKHSVSTSNGQRITKDLVLDILNTCTDKHL-AQVKGANKYALAKDVTADILTGPTFAEFLTLPCYPHIV 527
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A1V9ZCF1_9STRA (Malate synthase (Fragment) n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZCF1_9STRA)

HSP 1 Score: 129 bits (323), Expect = 4.190e-31
Identity = 60/117 (51.28%), Postives = 81/117 (69.23%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKIS 627
            GLEEN++V + Y E+WLRGNGCIP++ KMEDAATAEISR QVWQW+ HG ST D ++IT   +  I E  T + ++A+G    K+ LA ++  N +T    DDFLT   YPH+V ++
Sbjct:  394 GLEENIDVSLVYTEAWLRGNGCIPLHNKMEDAATAEISRVQVWQWLRHGRSTEDKVKITKERILQILENCTKQRISAAGTTH-KYELAQQVMINALTGDVFDDFLTLPCYPHVVSLA 509
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A024TKX8_9STRA (Malate synthase n=5 Tax=Aphanomyces TaxID=100860 RepID=A0A024TKX8_9STRA)

HSP 1 Score: 129 bits (323), Expect = 5.490e-31
Identity = 64/114 (56.14%), Postives = 83/114 (72.81%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIV 618
            GL+EN++V + YVE+WLRGNGCIP++ KMEDAATAEISR QVWQW+ H AST++G  IT  LV +I +  T+K L A   G  K+ LA  +TA+++T P   +FLT   YPHIV
Sbjct:  417 GLQENIDVTLVYVEAWLRGNGCIPLHNKMEDAATAEISRVQVWQWLKHAASTANGQRITKDLVLNILDDCTNKHL-ARAKGDHKYALAKDVTADILTGPTFPEFLTLPCYPHIV 529
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A1V9ZVL8_9STRA (Malate synthase n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZVL8_9STRA)

HSP 1 Score: 128 bits (322), Expect = 7.090e-31
Identity = 63/116 (54.31%), Postives = 80/116 (68.97%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKI 624
            GLEEN++V + Y E+WLRGNGCIP++ KMEDAATAEISR QVWQW+ H  STSDG  IT +LV DI    T K   A+ G   K++LA  +  N++T  + + FLT   YPHIV +
Sbjct:  411 GLEENIDVSLVYTEAWLRGNGCIPLHNKMEDAATAEISRVQVWQWLRHNRSTSDGKVITKQLVLDILASCTQKRAAATKGAN-KYQLAHDILVNVLTGDKFEAFLTLPCYPHIVTL 525
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A8K1C2B5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1C2B5_PYTOL)

HSP 1 Score: 128 bits (322), Expect = 7.390e-31
Identity = 61/116 (52.59%), Postives = 82/116 (70.69%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKI 624
            GLEEN++V + Y E+WLRG+GCIP++ KMEDAATAEISRAQVWQWV HG+ T +G  +T  LV  I +    K  + +  GG K+ LAGR+   ++T  ++ DFLT   YPHIV++
Sbjct:  418 GLEENIDVSLVYSEAWLRGSGCIPLHNKMEDAATAEISRAQVWQWVRHGSKTVEGKVVTKSLVLSILDECV-KRRSVNAKGGNKWALAGRIVGKILTDDDMVDFLTLPCYPHIVQV 532
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A225X4Z8_9STRA (Malate synthase n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225X4Z8_9STRA)

HSP 1 Score: 119 bits (297), Expect = 3.070e-30
Identity = 58/117 (49.57%), Postives = 79/117 (67.52%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKL-LNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKI 624
            GL EN++V + Y E+WLRG+GCIP++ KMEDAATAEISRAQVWQW+ H   T +G  +T  LV DI +   +K  LNA+ G   K+ L G +   ++T  ++ DFLT   YP IV++
Sbjct:   40 GLVENIDVSLVYTEAWLRGSGCIPLHNKMEDAATAEISRAQVWQWIRHSCKTVEGKTVTKELVLDILKECVNKRSLNAATGN--KWALGGEVMGKVLTGDDMVDFLTLPCYPRIVQL 154
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A485KK51_9STRA (Malate synthase n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KK51_9STRA)

HSP 1 Score: 126 bits (316), Expect = 4.900e-30
Identity = 60/116 (51.72%), Postives = 78/116 (67.24%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKI 624
            GLEEN++V + Y E+WLRG GCIP++ KMEDAATAEISR+QVWQW HH   T+DG  IT  L+  I + AT K   A+   G KF+LA  + A  +T    +DFLT   YPH++ +
Sbjct:  416 GLEENIDVALVYTEAWLRGIGCIPLHNKMEDAATAEISRSQVWQWRHHNCVTADGKSITKELILAILDTATEKRA-AAAASGHKFQLAKEVIAQSLTGVHFEDFLTLPCYPHVISL 530
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A5D6XJD5_9STRA (Malate synthase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XJD5_9STRA)

HSP 1 Score: 125 bits (314), Expect = 9.690e-30
Identity = 60/119 (50.42%), Postives = 83/119 (69.75%), Query Frame = 1 
Query:  271 QHGLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDI-AERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKI 624
            + GLEEN++V + Y E+WLRG+GCIP++ KMEDAATAEISRAQVWQW+ HG +T DG  +T  LV++I A+    + +NA  G   K+ L G +   ++T  ++ DFLT   YPHIV+ 
Sbjct:  416 RRGLEENIDVSLVYTEAWLRGSGCIPLHNKMEDAATAEISRAQVWQWIRHGCTTVDGTRVTKALVREILAKCVATRSVNARAGN--KWALGGAVVGKILTDDDMVDFLTLPCYPHIVQF 532
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BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Match: A0A067CE19_SAPPC (Malate synthase n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067CE19_SAPPC)

HSP 1 Score: 125 bits (313), Expect = 1.320e-29
Identity = 60/117 (51.28%), Postives = 81/117 (69.23%), Query Frame = 1 
Query:  277 GLEENVEVCIFYVESWLRGNGCIPVNFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLLNASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKIS 627
            GLEEN++V + Y E+WLRGNGCIP++ KMEDAATAEISR QVWQW+ HG ST+DG  IT  LV  I    T K +  +  G  K++LA  +  +++T+ + + FLT   YPHIV ++
Sbjct:  414 GLEENIDVSLEYTEAWLRGNGCIPLHNKMEDAATAEISRVQVWQWLRHGKSTTDGKRITKELVLSILAACTQKRVTQAKAGN-KYQLAHDVLVDVLTSDKFEAFLTLPCYPHIVTLA 529
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig17212.5107.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LTY7_ECTSI4.570e-7697.58Malate synthase n=2 Tax=Ectocarpus TaxID=2879 RepI... [more]
A0A485LAT4_9STRA1.470e-3157.89Malate synthase n=2 Tax=Aphanomyces TaxID=100860 R... [more]
A0A1V9ZCF1_9STRA4.190e-3151.28Malate synthase (Fragment) n=1 Tax=Thraustotheca c... [more]
A0A024TKX8_9STRA5.490e-3156.14Malate synthase n=5 Tax=Aphanomyces TaxID=100860 R... [more]
A0A1V9ZVL8_9STRA7.090e-3154.31Malate synthase n=1 Tax=Thraustotheca clavata TaxI... [more]
A0A8K1C2B5_PYTOL7.390e-3152.59Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A225X4Z8_9STRA3.070e-3049.57Malate synthase n=1 Tax=Phytophthora megakarya Tax... [more]
A0A485KK51_9STRA4.900e-3051.72Malate synthase n=1 Tax=Aphanomyces stellatus TaxI... [more]
A0A5D6XJD5_9STRA9.690e-3050.42Malate synthase n=1 Tax=Pythium brassicum TaxID=14... [more]
A0A067CE19_SAPPC1.320e-2951.28Malate synthase n=3 Tax=Saprolegniaceae TaxID=4764... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig17212contigEcto-sp13_S_contig17212:763..1733 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop1
Start1
Seed ortholog score248.4
Seed ortholog evalue2e-63
Seed eggNOG ortholog2880.D8LTY7
Preferred nameMLS1
Model size686
KEGG rclassRC00004,RC00308,RC00311,RC00313,RC02747
KEGG koko:K01637,ko:K01638
KEGG ReactionR00472,R00479
KEGG Pathwayko00620,ko00630,ko01100,ko01110,ko01120,ko01200,map00620,map00630,map01100,map01110,map01120,map01200
KEGG ModuleM00012
Hectar predicted targeting categoryother localisation
GOsGO:0000255,GO:0000256,GO:0003674,GO:0003824,GO:0004451,GO:0004474,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005777,GO:0005782,GO:0005829,GO:0005975,GO:0006081,GO:0006082,GO:0006083,GO:0006097,GO:0006629,GO:0006631,GO:0006807,GO:0007275,GO:0007568,GO:0008150,GO:0008152,GO:0008340,GO:0009056,GO:0009062,GO:0009514,GO:0009987,GO:0010259,GO:0015976,GO:0016042,GO:0016054,GO:0016740,GO:0016746,GO:0016829,GO:0016830,GO:0016833,GO:0016999,GO:0017001,GO:0017144,GO:0019752,GO:0031907,GO:0031974,GO:0032501,GO:0032502,GO:0032787,GO:0034641,GO:0042579,GO:0042737,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0043603,GO:0043605,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044262,GO:0044270,GO:0044281,GO:0044282,GO:0044422,GO:0044424,GO:0044438,GO:0044439,GO:0044444,GO:0044446,GO:0044464,GO:0045733,GO:0046395,GO:0046483,GO:0046487,GO:0046700,GO:0046912,GO:0048856,GO:0070013,GO:0071704,GO:0072329,GO:1901360,GO:1901361,GO:1901564,GO:1901565,GO:1901575
Exons2
EggNOG free text desc.malate synthase activity
EggNOG OGsCOG2225@1,KOG1261@2759
Ec32 ortholog descriptionMalate synthase
Ec32 orthologEc-12_003860.1
EC2.3.3.9,4.1.3.1
Cds size441
COG Functional cat.C
BiGG ReactioniMM904.YIR031C,iND750.YIR031C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462899.977427-UTR-Ecto-sp13_S_contig17212:762..9631681462899.977427-UTR-Ecto-sp13_S_contig17212:762..963Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig17212 763..963 +
1681462900.0184386-UTR-Ecto-sp13_S_contig17212:1689..17331681462900.0184386-UTR-Ecto-sp13_S_contig17212:1689..1733Ectocarpus species13 EcNAP12_S_4_19mUTREcto-sp13_S_contig17212 1690..1733 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462899.9904196-CDS-Ecto-sp13_S_contig17212:963..11611681462899.9904196-CDS-Ecto-sp13_S_contig17212:963..1161Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17212 964..1161 +
1681462900.0018878-CDS-Ecto-sp13_S_contig17212:1446..16891681462900.0018878-CDS-Ecto-sp13_S_contig17212:1446..1689Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17212 1447..1689 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig17212.5107.1prot_Ecto-sp13_S_contig17212.5107.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig17212 964..1689 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig17212.5107.1

>prot_Ecto-sp13_S_contig17212.5107.1 ID=prot_Ecto-sp13_S_contig17212.5107.1|Name=mRNA_Ecto-sp13_S_contig17212.5107.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=147bp
MCSVLSAIDRRRSCPVRLLRDHRQHGLEENVEVCIFYVESWLRGNGCIPV
NFKMEDAATAEISRAQVWQWVHHGASTSDGIEITPRLVQDIAERATHKLL
NASGGGGGKFRLAGRLTANMMTAPELDDFLTSVAYPHIVKISHGRL*
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mRNA from alignment at Ecto-sp13_S_contig17212:763..1733+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig17212.5107.1 ID=mRNA_Ecto-sp13_S_contig17212.5107.1|Name=mRNA_Ecto-sp13_S_contig17212.5107.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=971bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17212:763..1733+ (Ectocarpus species13 EcNAP12_S_4_19m)
TGTACGTGTATCAAGCGCCTCGGCGGGTTTTTCCGGTACTCTCCGTGTGT
TCGATTTTACTGTGGGGCATTGGAATTTTGGAACGAATGTGCGAAACGTC
GCCGTCGTCATGAACATCTTGGTGCCGAAGTCGTGGCATTCACGCAGGAA
TGCCGAAGCCACATAAAAATACACCGTAACTTCGTCTTTTGGTGACGCAA
GATGTGTAGTGTCTTGTCTGCGATCGATCGTCGTCGTTCATGTCCCGTTC
GGCTGCTACGGGATCACAGGCAACACGGGTTGGAGGAAAACGTGGAGGTG
TGCATCTTCTACGTCGAGAGCTGGCTGCGCGGCAACGGCTGCATCCCGGT
CAACTTCAAGATGGAAGATGCCGCAACCGCTGAGATCTCTCGGGCGCAGG
TATCTACATAGGACGCGGCGTCCCCCCCCCCCCCTCCCCCGTGTCCCCCT
TCTGACCCACTCCTCCCATTGGTCGACACGCTAGCGACCTAGCCTCACGC
ACCCAATCAGATTGATAGCAGTTGCTTGCTATGGTGCATATATACCCCGG
ATTTTTATATGCATTAAAAGAAAAAACAATTTGAACGGTGGCCTTATCGC
TCGATGGTACTGATTGCCGGCGTGTGCGCACTCGTTGTTTTTCTTTGCCT
TCGCATGCGCGTGTATCGTCATTCCGAACCAAAGGTCTGGCAATGGGTCC
ACCACGGCGCTTCCACGTCCGACGGCATCGAGATTACCCCTCGCCTCGTG
CAAGATATCGCGGAGAGGGCCACCCACAAACTCCTAAACGCCTCCGGGGG
CGGCGGCGGCAAGTTTCGCCTGGCTGGGCGGCTGACAGCGAACATGATGA
CCGCGCCCGAGCTGGACGACTTCCTCACGTCCGTGGCCTACCCCCATATC
GTCAAAATCTCGCACGGCCGTCTGTAGGAGAGAACGAGAAAGAGAGAGAG
AGAGAGAGATCCTTCCACCGT
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig17212:763..1733+

>mRNA_Ecto-sp13_S_contig17212.5107.1 ID=mRNA_Ecto-sp13_S_contig17212.5107.1|Name=mRNA_Ecto-sp13_S_contig17212.5107.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=441bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17212:763..1733+ (Ectocarpus species13 EcNAP12_S_4_19m)
ATGTGTAGTGTCTTGTCTGCGATCGATCGTCGTCGTTCATGTCCCGTTCG
GCTGCTACGGGATCACAGGCAACACGGGTTGGAGGAAAACGTGGAGGTGT
GCATCTTCTACGTCGAGAGCTGGCTGCGCGGCAACGGCTGCATCCCGGTC
AACTTCAAGATGGAAGATGCCGCAACCGCTGAGATCTCTCGGGCGCAGGT
CTGGCAATGGGTCCACCACGGCGCTTCCACGTCCGACGGCATCGAGATTA
CCCCTCGCCTCGTGCAAGATATCGCGGAGAGGGCCACCCACAAACTCCTA
AACGCCTCCGGGGGCGGCGGCGGCAAGTTTCGCCTGGCTGGGCGGCTGAC
AGCGAACATGATGACCGCGCCCGAGCTGGACGACTTCCTCACGTCCGTGG
CCTACCCCCATATCGTCAAAATCTCGCACGGCCGTCTGTAG
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