mRNA_Ecto-sp13_S_contig17200.5101.1 (mRNA) Ectocarpus species13 EcNAP12_S_4_19m

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_Ecto-sp13_S_contig17200.5101.1
Unique NamemRNA_Ecto-sp13_S_contig17200.5101.1
TypemRNA
OrganismEctocarpus species13 EcNAP12_S_4_19m (Ectocarpus species13 EcNAP12_S_4_19m)
Homology
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: D8LFY1_ECTSI (Diphthine--ammonia ligase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFY1_ECTSI)

HSP 1 Score: 178 bits (451), Expect = 2.050e-50
Identity = 93/95 (97.89%), Postives = 94/95 (98.95%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRL LTSLAYLWRRPQSPLLAEMVEAGL+AVLVKV
Sbjct:   24 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLGLTSLAYLWRRPQSPLLAEMVEAGLNAVLVKV 118
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: I1CIS5_RHIO9 (Diphthine--ammonia ligase n=1 Tax=Rhizopus delemar (strain RA 99-880 / ATCC MYA-4621 / FGSC 9543 / NRRL 43880) TaxID=246409 RepID=I1CIS5_RHIO9)

HSP 1 Score: 120 bits (302), Expect = 1.940e-32
Identity = 59/95 (62.11%), Postives = 75/95 (78.95%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            RE+ G A++   QY +T  DE EDL+ LLKE+KE+ PEV+GV SGAILS+YQ+ RVENVC RL LTSLAYLW R Q  LLAEM ++G++A+L+KV
Sbjct:   55 REITGTAISLGAQYEVTPKDETEDLYELLKEIKEKHPEVQGVSSGAILSSYQKVRVENVCDRLGLTSLAYLWERNQKELLAEMADSGVNAILIKV 149
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: L8GRE2_ACACA (Diphthine--ammonia ligase n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GRE2_ACACA)

HSP 1 Score: 121 bits (304), Expect = 1.390e-31
Identity = 61/93 (65.59%), Postives = 75/93 (80.65%), Query Frame = 1 
Query:    7 LAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            + G+A+NQ L Y+ T++DEVE L++LLKEVK+RFP V+GV SGAILS+YQR RVENVC RL L SLAYLW+R Q  LL EM E  +DAVL+KV
Sbjct:   59 IRGKALNQDLHYAKTQEDEVESLYVLLKEVKKRFPNVQGVASGAILSDYQRFRVENVCERLGLVSLAYLWQRDQKELLQEMTEVPIDAVLIKV 151
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: A0A397WC71_9GLOM (Diphthine--ammonia ligase n=1 Tax=Gigaspora rosea TaxID=44941 RepID=A0A397WC71_9GLOM)

HSP 1 Score: 117 bits (293), Expect = 4.420e-31
Identity = 60/95 (63.16%), Postives = 73/95 (76.84%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            R + GR++ Q   Y LT  DE EDLF LLKEVKE  P+++GV  GAILSNYQR RVENVC RL LT LAYLWRR Q  LL+EM++AG++A+L+KV
Sbjct:   55 RGIFGRSLIQDADYKLTIGDETEDLFELLKEVKESNPDIQGVSVGAILSNYQRVRVENVCSRLGLTPLAYLWRRNQKELLSEMIDAGINAILIKV 149
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: G3ALH6_SPAPN (Diphthine--ammonia ligase (Fragment) n=1 Tax=Spathaspora passalidarum (strain NRRL Y-27907 / 11-Y1) TaxID=619300 RepID=G3ALH6_SPAPN)

HSP 1 Score: 114 bits (286), Expect = 5.340e-31
Identity = 56/93 (60.22%), Postives = 72/93 (77.42%), Query Frame = 1 
Query:    7 LAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            + G + NQ L+YS T++DE+EDLFLLL  +K++ PE+EGV  GAILS+YQRTRVENVC RL LTSL YLW+R Q  L+ EM  A +DA ++KV
Sbjct:   26 ITGNSANQNLEYSPTDNDEIEDLFLLLSNIKQQHPEIEGVSCGAILSHYQRTRVENVCERLGLTSLTYLWQRGQDELMREMCVAQMDARIIKV 118
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: A0A0V1PY81_9ASCO (Diphthine--ammonia ligase n=1 Tax=Debaryomyces fabryi TaxID=58627 RepID=A0A0V1PY81_9ASCO)

HSP 1 Score: 121 bits (304), Expect = 3.720e-30
Identity = 61/95 (64.21%), Postives = 76/95 (80.00%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            +E+ G + NQ L+YS+TE+DE+EDL+LLLK VKE+ P+VEGV  GAILS+YQRTRVENVCGRL LT LAYLW+R Q  L+ EM    LDA ++KV
Sbjct:   70 QEINGSSKNQRLEYSITEEDEIEDLYLLLKTVKEKHPDVEGVSCGAILSHYQRTRVENVCGRLGLTCLAYLWQRNQLELMGEMCNNQLDARIIKV 164
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: Q6BX95_DEBHA (Diphthine--ammonia ligase n=1 Tax=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) TaxID=284592 RepID=Q6BX95_DEBHA)

HSP 1 Score: 121 bits (304), Expect = 3.800e-30
Identity = 60/95 (63.16%), Postives = 77/95 (81.05%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            +E+ G + NQ L+YS+TE+DE+EDL++LLK+VKE+ P+VEGV  GAILS+YQRTRVENVCGRL LT LAYLW+R Q  L+ EM    LDA ++KV
Sbjct:   70 QEINGSSKNQLLEYSITEEDEIEDLYVLLKKVKEKHPDVEGVSCGAILSHYQRTRVENVCGRLGLTCLAYLWQRDQLELMGEMCNNQLDARIIKV 164
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: A0A4P9YU66_9FUNG (Diphthine--ammonia ligase n=1 Tax=Syncephalis pseudoplumigaleata TaxID=1712513 RepID=A0A4P9YU66_9FUNG)

HSP 1 Score: 114 bits (286), Expect = 5.020e-30
Identity = 59/95 (62.11%), Postives = 71/95 (74.74%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            RE+ G    QAL Y  T DDE EDL+ LLKEVK+R+P VE V  GAILSNYQR RVE+VC RL LTSLAYLW++ Q+ LL  M++ GL A+L+KV
Sbjct:   55 REIHGTPKRQALDYETTIDDETEDLYALLKEVKQRYPAVEAVSVGAILSNYQRIRVEHVCQRLGLTSLAYLWQQDQAELLNSMIDNGLQAILIKV 149
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: A0A2G5VI07_9PELO (Diphthine--ammonia ligase n=2 Tax=Caenorhabditis TaxID=6237 RepID=A0A2G5VI07_9PELO)

HSP 1 Score: 115 bits (289), Expect = 5.330e-30
Identity = 60/95 (63.16%), Postives = 74/95 (77.89%), Query Frame = 1 
Query:    1 RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            RE++G+A NQ   Y  TE DEVEDLF LL EVK+  PEV+GV +GAILS+YQ+ RVE+VC RL LT L +LW R Q+ LL+EMVE G+DAVL+KV
Sbjct:   71 REISGQAKNQKANYEKTEGDEVEDLFELLTEVKKNHPEVKGVSAGAILSSYQKVRVEDVCRRLDLTPLCFLWEREQNSLLSEMVENGVDAVLIKV 165
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BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Match: A0A3L6V4S0_9STRA (Diphthine--ammonia ligase n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A3L6V4S0_9STRA)

HSP 1 Score: 114 bits (284), Expect = 5.640e-30
Identity = 58/93 (62.37%), Postives = 72/93 (77.42%), Query Frame = 1 
Query:    7 LAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSNYQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV 285
            L G +V+  + Y  TE DEVEDL  LL+EV+ +FP+V+ V SGAI SNYQRTRVE+VC RL LTSLAYLWRRPQ  L+ EM++ G+ A+LVKV
Sbjct:   71 LQGTSVSTDMGYDTTEGDEVEDLLRLLEEVQRQFPDVQAVSSGAIFSNYQRTRVEHVCARLNLTSLAYLWRRPQHELMDEMLDRGVHAILVKV 163
The following BLAST results are available for this feature:
BLAST of mRNA_Ecto-sp13_S_contig17200.5101.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LFY1_ECTSI2.050e-5097.89Diphthine--ammonia ligase n=1 Tax=Ectocarpus silic... [more]
I1CIS5_RHIO91.940e-3262.11Diphthine--ammonia ligase n=1 Tax=Rhizopus delemar... [more]
L8GRE2_ACACA1.390e-3165.59Diphthine--ammonia ligase n=1 Tax=Acanthamoeba cas... [more]
A0A397WC71_9GLOM4.420e-3163.16Diphthine--ammonia ligase n=1 Tax=Gigaspora rosea ... [more]
G3ALH6_SPAPN5.340e-3160.22Diphthine--ammonia ligase (Fragment) n=1 Tax=Spath... [more]
A0A0V1PY81_9ASCO3.720e-3064.21Diphthine--ammonia ligase n=1 Tax=Debaryomyces fab... [more]
Q6BX95_DEBHA3.800e-3063.16Diphthine--ammonia ligase n=1 Tax=Debaryomyces han... [more]
A0A4P9YU66_9FUNG5.020e-3062.11Diphthine--ammonia ligase n=1 Tax=Syncephalis pseu... [more]
A0A2G5VI07_9PELO5.330e-3063.16Diphthine--ammonia ligase n=2 Tax=Caenorhabditis T... [more]
A0A3L6V4S0_9STRA5.640e-3062.37Diphthine--ammonia ligase n=1 Tax=Aphanomyces asta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
Ecto-sp13_S_contig17200contigEcto-sp13_S_contig17200:2503..3194 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Ectocarpus species13 EcNAP12_S_4_19m OGS1.02022-07-08
Properties
Property NameValue
Taxonomic scopeEukaryota
Stop0
Start0
Seed ortholog score180.3
Seed ortholog evalue4.4e-43
Seed eggNOG ortholog2880.D8LFY1
Preferred nameHRSP12
Model size285
KEGG rclassRC00296,RC00358,RC01015,RC03275,RC03354
KEGG koko:K06927,ko:K06965,ko:K09022,ko:K13761,ko:K15067,ko:K19783
KEGG ReactionR01234,R03613,R03887,R11098,R11099
KEGG Pathwayko00230,ko00380,ko03015,map00230,map00380,map03015
Hectar predicted targeting categoryother localisation
GOsGO:0001505,GO:0001655,GO:0001822,GO:0003674,GO:0003824,GO:0004518,GO:0004519,GO:0004521,GO:0004540,GO:0005488,GO:0005504,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005730,GO:0005737,GO:0005739,GO:0005740,GO:0005758,GO:0005759,GO:0005773,GO:0005777,GO:0005829,GO:0006082,GO:0006139,GO:0006412,GO:0006417,GO:0006448,GO:0006457,GO:0006464,GO:0006518,GO:0006520,GO:0006544,GO:0006549,GO:0006566,GO:0006567,GO:0006725,GO:0006807,GO:0007049,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0008150,GO:0008152,GO:0008285,GO:0008289,GO:0008652,GO:0009056,GO:0009058,GO:0009059,GO:0009063,GO:0009066,GO:0009068,GO:0009069,GO:0009081,GO:0009082,GO:0009097,GO:0009507,GO:0009526,GO:0009532,GO:0009536,GO:0009570,GO:0009579,GO:0009889,GO:0009890,GO:0009892,GO:0009941,GO:0009987,GO:0010033,GO:0010035,GO:0010038,GO:0010467,GO:0010468,GO:0010556,GO:0010558,GO:0010605,GO:0010608,GO:0010629,GO:0016053,GO:0016054,GO:0016070,GO:0016787,GO:0016788,GO:0016874,GO:0016879,GO:0016880,GO:0016892,GO:0016894,GO:0017144,GO:0017148,GO:0017178,GO:0017182,GO:0017183,GO:0018193,GO:0018202,GO:0019222,GO:0019239,GO:0019518,GO:0019538,GO:0019752,GO:0022402,GO:0030323,GO:0030324,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031406,GO:0031967,GO:0031970,GO:0031974,GO:0031975,GO:0031981,GO:0032268,GO:0032269,GO:0032501,GO:0032502,GO:0032543,GO:0033293,GO:0033993,GO:0034248,GO:0034249,GO:0034641,GO:0034645,GO:0035295,GO:0036041,GO:0036094,GO:0036211,GO:0042127,GO:0042133,GO:0042221,GO:0042579,GO:0042737,GO:0042802,GO:0042803,GO:0043043,GO:0043167,GO:0043168,GO:0043169,GO:0043170,GO:0043177,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043412,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044267,GO:0044271,GO:0044281,GO:0044282,GO:0044283,GO:0044422,GO:0044424,GO:0044428,GO:0044429,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046394,GO:0046395,GO:0046483,GO:0046686,GO:0046872,GO:0046914,GO:0046983,GO:0048513,GO:0048519,GO:0048523,GO:0048731,GO:0048856,GO:0050678,GO:0050680,GO:0050789,GO:0050794,GO:0050896,GO:0051171,GO:0051172,GO:0051246,GO:0051248,GO:0060255,GO:0060322,GO:0060541,GO:0065007,GO:0065008,GO:0070013,GO:0070314,GO:0071704,GO:0072001,GO:0080090,GO:0090304,GO:0090305,GO:0090501,GO:0090502,GO:0140053,GO:0140098,GO:1900247,GO:1901360,GO:1901564,GO:1901565,GO:1901566,GO:1901575,GO:1901576,GO:1901605,GO:1901606,GO:1901607,GO:1902074,GO:1904012,GO:1904013,GO:2000112,GO:2000113,GO:2000765
Exons2
EggNOG free text desc.diphthine-ammonia ligase activity
EggNOG OGsCOG0251@1,COG2102@1,KOG2316@2759,KOG2317@2759
EC3.1.4.35,3.5.99.10,3.5.99.5,3.6.4.12,6.3.1.14
Cds size285
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03012,ko03032
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1681462899.5755446-CDS-Ecto-sp13_S_contig17200:2502..26011681462899.5755446-CDS-Ecto-sp13_S_contig17200:2502..2601Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17200 2503..2601 +
1681462899.587991-CDS-Ecto-sp13_S_contig17200:3008..31941681462899.587991-CDS-Ecto-sp13_S_contig17200:3008..3194Ectocarpus species13 EcNAP12_S_4_19mCDSEcto-sp13_S_contig17200 3009..3194 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_Ecto-sp13_S_contig17200.5101.1prot_Ecto-sp13_S_contig17200.5101.1Ectocarpus species13 EcNAP12_S_4_19mpolypeptideEcto-sp13_S_contig17200 2503..3194 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_Ecto-sp13_S_contig17200.5101.1

>prot_Ecto-sp13_S_contig17200.5101.1 ID=prot_Ecto-sp13_S_contig17200.5101.1|Name=mRNA_Ecto-sp13_S_contig17200.5101.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=polypeptide|length=95bp
RELAGRAVNQALQYSLTEDDEVEDLFLLLKEVKERFPEVEGVGSGAILSN
YQRTRVENVCGRLALTSLAYLWRRPQSPLLAEMVEAGLDAVLVKV
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mRNA from alignment at Ecto-sp13_S_contig17200:2503..3194+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_Ecto-sp13_S_contig17200.5101.1 ID=mRNA_Ecto-sp13_S_contig17200.5101.1|Name=mRNA_Ecto-sp13_S_contig17200.5101.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=mRNA|length=692bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17200:2503..3194+ (Ectocarpus species13 EcNAP12_S_4_19m)
AGGGAGCTCGCGGGGAGGGCCGTGAACCAAGCCCTCCAGTACAGCCTCAC
CGAAGATGATGAGGTGGAAGACCTATTTCTACTCCTGAAGGAAGTCAAGG
TACCCGTTCTATGCCGTACGCACCTCTTGGCGCGTTCGAGTCGTTTTATT
GGCTTCGAGTTGCCGAGCGGATATAATGGTGAAGTGTTCGTTTACCGCCC
ACTGTTGGTGGCGGTGATAATATTGTTGTTGGCCGTAGGTGCGGGAGGAA
TCGGTGGTGGAGATGGGGATGGTGTTGGTACTACTACAAATATCGGTGTT
TGATTATGAGGGTGTCGCCATTAGGGTGGTTTCGTGTCCGCGTTGACGAC
GCGGTGGTTTACCAAGAATATTTGGTGGCCAGTGATAGGTTTCTCCGGCA
TCATGTTGCCTGGTTGGCGTACGGCTGTGTTGCCCTCGCCGTCGACACCC
GCACCGCTGCCATCAAAAGTTGCTATGCCACACAACGCGCGCGACCATCT
CAACAGGAACGCTTCCCGGAGGTGGAGGGGGTCGGTTCCGGCGCTATCTT
GTCCAACTACCAGCGCACTCGCGTGGAGAACGTCTGCGGGAGGCTGGCCC
TGACGTCCCTCGCTTACCTGTGGAGACGACCGCAGTCTCCTCTGCTGGCG
GAGATGGTGGAAGCCGGCCTGGATGCTGTGTTGGTGAAGGTG
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Coding sequence (CDS) from alignment at Ecto-sp13_S_contig17200:2503..3194+

>mRNA_Ecto-sp13_S_contig17200.5101.1 ID=mRNA_Ecto-sp13_S_contig17200.5101.1|Name=mRNA_Ecto-sp13_S_contig17200.5101.1|organism=Ectocarpus species13 EcNAP12_S_4_19m|type=CDS|length=285bp|location=Sequence derived from alignment at Ecto-sp13_S_contig17200:2503..3194+ (Ectocarpus species13 EcNAP12_S_4_19m)
AGGGAGCTCGCGGGGAGGGCCGTGAACCAAGCCCTCCAGTACAGCCTCAC
CGAAGATGATGAGGTGGAAGACCTATTTCTACTCCTGAAGGAAGTCAAGG
AACGCTTCCCGGAGGTGGAGGGGGTCGGTTCCGGCGCTATCTTGTCCAAC
TACCAGCGCACTCGCGTGGAGAACGTCTGCGGGAGGCTGGCCCTGACGTC
CCTCGCTTACCTGTGGAGACGACCGCAGTCTCCTCTGCTGGCGGAGATGG
TGGAAGCCGGCCTGGATGCTGTGTTGGTGAAGGTG
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